Cyct cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyct
Entrez Gene ID	13067
Full Name	cytochrome c, testis
Synonyms	T-Cc
General protein information	Preferred Names cytochrome c, testis Names cytochrome c, testis-specific
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 C3\|2 44.94 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009989.3	NP_034119.1	cytochrome c, testis-specific
XM_011239273.1	XP_011237575.1	cytochrome c, testis-specific isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC100613341	XP_003309478.1
Rattus norvegicus (Norway rat)	Cyct	NP_036972.1
Oryza sativa (rice)	Os05g0420600	NP_001055578.1
Mus musculus (house mouse)	Cyct	NP_034119.1
Arabidopsis thaliana (thale cress)	CYTC-1	NP_173697.1
Bos taurus (cattle)	CYCT	NP_001071431.1
Arabidopsis thaliana (thale cress)	CYTC-2	NP_192742.1

Gene Ontology
Bibliography

Related articles in PubMed

EHMT2 directs DNA methylation for efficient gene silencing in mouse embryos.
Auclair G, Borgel J, Sanz LA, Vallet J, Guibert S, Dumas M, Cavelier P, Girardot M, Forn? T, Feil R, Weber M
Genome research26(2)192-202(2016 Feb)

Lack of cytochrome c in mouse fibroblasts disrupts assembly/stability of respiratory complexes I and IV.
Vempati UD, Han X, Moraes CT
The Journal of biological chemistry284(7)4383-91(2009 Feb)

Role of cytochrome C in apoptosis: increased sensitivity to tumor necrosis factor alpha is associated with respiratory defects but not with lack of cytochrome C release.
Vempati UD, Diaz F, Barrientos A, Narisawa S, Mian AM, Mill?n JL, Boise LH, Moraes CT
Molecular and cellular biology27(5)1771-83(2007 Mar)

Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis.
Liu Z, Lin H, Ye S, Liu QY, Meng Z, Zhang CM, Xia Y, Margoliash E, Rao Z, Liu XJ
Proceedings of the National Academy of Sciences of the United States of America103(24)8965-70(2006 Jun)

The murine immune response to the male-specific antigen mouse testicular cytochrome c.
Kelner GS, Minnerath JM, Jenkins MK, Jemmerson R
European journal of immunology23(8)1992-8(1993 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

T-Cc can catalyze reduction of H(2)O(2) 3 times faster than somatic cell Cc(S-Cc); T-Cc heme has greater resistance to degradation by H(2)O(2); implies T-Cc can protect sperm from H(2)O(2)damage; T-Cc apoptotic activity is 3 to 5 times greater than S-Cc
Title: Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis.

These findings suggest that heat stress of testes triggers the release of cytochrome c from mitochondria in spermatogenic cells, leading to the activation of an apoptotic pathway.
Title: Suppression of cytochrome c release and apoptosis in testes with heat stress by minocycline.

The following Cyct gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyct cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15107	NM_009989.3 Latest version!	Mus musculus cytochrome c, testis (Cyct), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu15107	XM_011239273.1 Latest version!	Mus musculus cytochrome c, testis (Cyct), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyct ( NM_009989.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034119.1 Mus musculus cytochrome c, testis (Cyct), mRNA.

Price & Availability↑

CloneID	OMu15107
Clone ID Related Accession (Same CDS sequence)	NM_009989.3 , XM_011239273.1
Accession Version	NM_009989.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	cytochrome c, testis-specific
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL845373.12 and BC049608.1. On Jul 31, 2014 this sequence version replaced NM_009989.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC049608.1, BC059728.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164133 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta regulatory uORF :: PMID: 7984407 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGAGATG CTGAAGCAGG CAAGAAGATC TTTGTTCAGA AATGTGCTCA GTGCCACACG 
GTGGAAAAAG GCGGGAAACA CAAGACAGGT CCAAATCTCT GGGGCCTCTT TGGTCGAAAG 
ACAGGACAAG CACCAGGATT TTCTTACACG GATGCAAACA AGAACAAAGG TGTTATCTGG 
TCAGAAGAAA CTTTGATGGA ATATTTGGAG AACCCGAAGA AATATATCCC TGGCACTAAA 
ATGATCTTTG CTGGTATCAA AAAGAAGAGT GAGAGAGAAG ATCTCATTAA GTATTTAAAA 
CAGGCCACAT CCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034119.1
CDS	195..512
Translation	MGDAEAGKKIFVQKCAQCHTVEKGGKHKTGPNLWGLFGRKTGQAPGFSYTDANKNKGVIWSEETLMEYLENPKKYIPGTKMIFAGIKKKSEREDLIKYLKQATSS

Target ORF information:

RefSeq Version	NM_009989.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome c, testis (Cyct), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009989.3

ORF Insert Sequence:

ATGGGAGATG CTGAAGCAGG CAAGAAGATC TTTGTTCAGA AATGTGCTCA GTGCCACACG 
GTGGAAAAAG GCGGGAAACA CAAGACAGGT CCAAATCTCT GGGGCCTCTT TGGTCGAAAG 
ACAGGACAAG CACCAGGATT TTCTTACACG GATGCAAACA AGAACAAAGG TGTTATCTGG 
TCAGAAGAAA CTTTGATGGA ATATTTGGAG AACCCGAAGA AATATATCCC TGGCACTAAA 
ATGATCTTTG CTGGTATCAA AAAGAAGAGT GAGAGAGAAG ATCTCATTAA GTATTTAAAA 
CAGGCCACAT CCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyct ( XM_011239273.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237575.1 Mus musculus cytochrome c, testis (Cyct), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15107
Clone ID Related Accession (Same CDS sequence)	NM_009989.3 , XM_011239273.1
Accession Version	XM_011239273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cytochrome c, testis-specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG CTGAAGCAGG CAAGAAGATC TTTGTTCAGA AATGTGCTCA GTGCCACACG 
GTGGAAAAAG GCGGGAAACA CAAGACAGGT CCAAATCTCT GGGGCCTCTT TGGTCGAAAG 
ACAGGACAAG CACCAGGATT TTCTTACACG GATGCAAACA AGAACAAAGG TGTTATCTGG 
TCAGAAGAAA CTTTGATGGA ATATTTGGAG AACCCGAAGA AATATATCCC TGGCACTAAA 
ATGATCTTTG CTGGTATCAA AAAGAAGAGT GAGAGAGAAG ATCTCATTAA GTATTTAAAA 
CAGGCCACAT CCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237575.1
CDS	348..665
Translation	MGDAEAGKKIFVQKCAQCHTVEKGGKHKTGPNLWGLFGRKTGQAPGFSYTDANKNKGVIWSEETLMEYLENPKKYIPGTKMIFAGIKKKSEREDLIKYLKQATSS

Target ORF information:

RefSeq Version	XM_011239273.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome c, testis (Cyct), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239273.1

ORF Insert Sequence:

ATGGGAGATG CTGAAGCAGG CAAGAAGATC TTTGTTCAGA AATGTGCTCA GTGCCACACG 
GTGGAAAAAG GCGGGAAACA CAAGACAGGT CCAAATCTCT GGGGCCTCTT TGGTCGAAAG 
ACAGGACAAG CACCAGGATT TTCTTACACG GATGCAAACA AGAACAAAGG TGTTATCTGG 
TCAGAAGAAA CTTTGATGGA ATATTTGGAG AACCCGAAGA AATATATCCC TGGCACTAAA 
ATGATCTTTG CTGGTATCAA AAAGAAGAGT GAGAGAGAAG ATCTCATTAA GTATTTAAAA 
CAGGCCACAT CCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EHMT2 directs DNA methylation for efficient gene silencing in mouse embryos.
Genome research26(2)192-202(2016 Feb)
Auclair G,Borgel J,Sanz LA,Vallet J,Guibert S,Dumas M,Cavelier P,Girardot M,Forn? T,Feil R,Weber M

Lack of cytochrome c in mouse fibroblasts disrupts assembly/stability of respiratory complexes I and IV.
The Journal of biological chemistry284(7)4383-91(2009 Feb)
Vempati UD,Han X,Moraes CT

Role of cytochrome C in apoptosis: increased sensitivity to tumor necrosis factor alpha is associated with respiratory defects but not with lack of cytochrome C release.
Molecular and cellular biology27(5)1771-83(2007 Mar)
Vempati UD,Diaz F,Barrientos A,Narisawa S,Mian AM,Mill?n JL,Boise LH,Moraes CT

Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis.
Proceedings of the National Academy of Sciences of the United States of America103(24)8965-70(2006 Jun)
Liu Z,Lin H,Ye S,Liu QY,Meng Z,Zhang CM,Xia Y,Margoliash E,Rao Z,Liu XJ

The murine immune response to the male-specific antigen mouse testicular cytochrome c.
European journal of immunology23(8)1992-8(1993 Aug)
Kelner GS,Minnerath JM,Jenkins MK,Jemmerson R