Coch cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Coch
Entrez Gene ID	12810
Full Name	cochlin
Synonyms	AW122937,Coch-5B2,D12H14S564E
General protein information	Preferred Names cochlin Names cochlin coagulation factor C homolog (Limulus polyphemus)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 B3\|12 22.11 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017314943.1	XP_017170432.1	cochlin isoform X1
NM_007728.5	NP_031754.1	cochlin precursor
NM_001198835.1	NP_001185764.1	cochlin precursor
XM_017314943.2	XP_017170432.1	cochlin isoform X1
XM_017314944.1	XP_017170433.1	cochlin isoform X1

Pathways from BioSystems

Homologies

	COCH	XP_001114797.1
Bos taurus (cattle)	COCH	NP_001071310.1
Gallus gallus (chicken)	COCH	NP_990268.1
Danio rerio (zebrafish)	coch	NP_001003823.1
Xenopus tropicalis (tropical clawed frog)	coch	XP_004917329.1
Pan troglodytes (chimpanzee)	COCH	XP_001171057.1
Canis lupus familiaris (dog)	COCH	XP_547762.4
Homo sapiens (human)	COCH	NP_001128530.1
Mus musculus (house mouse)	Coch	NP_031754.1

Gene Ontology
Bibliography

Related articles in PubMed

Cochlin in normal middle ear and abnormal middle ear deposits in DFNA9 and Coch (G88E/G88E) mice.
Robertson NG, O'Malley JT, Ong CA, Giersch AB, Shen J, Stankovic KM, Morton CC
Journal of the Association for Research in Otolaryngology : JARO15(6)961-74(2014 Dec)

Resistance to noise-induced hearing loss in 129S6 and MOLF mice: identification of independent, overlapping, and interacting chromosomal regions.
Street VA, Kujawa SG, Manichaikul A, Broman KW, Kallman JC, Shilling DJ, Iwata AJ, Robinson LC, Robbins CA, Li J, Liberman MC, Tempel BL
Journal of the Association for Research in Otolaryngology : JARO15(5)721-38(2014 Oct)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Canonical Wnt signaling regulates the proliferative expansion and differentiation of fibrocytes in the murine inner ear.
Bohnenpoll T, Trowe MO, Wojahn I, Taketo MM, Petry M, Kispert A
Developmental biology391(1)54-65(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

accumulation of acellular deposits in the incudomalleal and incudostapedial joints in Coch (G88E/G88E) mice similar to those found in human DFNA9-affected temporal bones
Title: Cochlin in normal middle ear and abnormal middle ear deposits in DFNA9 and Coch (G88E/G88E) mice.

study reports that cochlin is expressed by follicular dendritic cells (FDCs) and selectively localized in the fine extracellular network of conduits in the spleen and lymph nodes; by producing cochlin, FDCs contribute to the innate immune response in defense against bacteria
Title: Cochlin produced by follicular dendritic cells promotes antibacterial innate immunity.

COCH is a part of the downstream target network of BMP4 signaling and serves as another important effector to fine-tune mouse embryonic stem cell fates.
Title: BMP induces cochlin expression to facilitate self-renewal and suppress neural differentiation of mouse embryonic stem cells.

Loss of Cochlin is associated with reduced intraocular pressure and mechanosensing.
Title: Cochlin, intraocular pressure regulation and mechanosensing.

In Coch knock-in/knockout mouse models of sensorineural hearing loss and vestibular dysfunction vestibular function is compromised before cochlear function.
Title: Hearing and vestibular deficits in the Coch(-/-) null mouse model: comparison to the Coch(G88E/G88E) mouse and to DFNA9 hearing and balance disorder.

The following Coch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Coch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22799	NM_001198835.1 Latest version!	Mus musculus cochlin (Coch), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu22799	NM_007728.5 Latest version!	Mus musculus cochlin (Coch), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu78133	XM_017314944.1 Latest version!	Mus musculus cochlin (Coch), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu78133	XM_017314943.2 Latest version!	Mus musculus cochlin (Coch), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu78133	XM_017314943.1	Mus musculus cochlin (Coch), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Coch ( NM_007728.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031754.1 Mus musculus cochlin (Coch), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22799
Clone ID Related Accession (Same CDS sequence)	NM_007728.5 , NM_001198835.1
Accession Version	NM_007728.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Mus musculus(house mouse)
Product	cochlin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK137336.1 and AK028690.1. On Nov 10, 2010 this sequence version replaced NM_007728.4. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK028690.1, AK137336.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGCCCTCGT CCAGGATCCC TGCTCTCTGC CTCGGTGCGT GGCTGCTGCT GCTGCTGCTG 
CCCCGGTTCG CGCGCGCCGA GGGAGCGGTT CCCATTCCTG TCACCTGCTT TACCAGAGGC 
CTGGATATCC GAAAAGAGAA AGCAGATGTT CTCTGCCCAG GAGGCTGCTC TCTTGAGGAA 
TTCTCTGTGT TTGGGAACAT AGTGTATGCG TCAGTGTCCA GCATCTGCGG CGCTGCTGTC 
CATAGGGGAG TGATTGGCAC CTCAGGGGGA CCTGTGCGTG TCTACAGCCT TCCTGGTCGA 
GAGAACTACT CCTCGGTAGA TGCCAACGGC ATCCAGTCTC AGATGCTTTC CCGATGGTCC 
GCGTCCTTCG CTGTGACCAA AGGCAAAAGC AGTACCCAGG AAGCCACAGG ACGGGCAGTG 
TCCACAGCCC ACCCACCTTC AGGTAAAAGA CTAAAGAAGA CACCAGAGAA GAAGACTGGC 
AACAAAGACT GTAAGGCAGA CATTGCATTT CTCATTGATG GAAGCTTCAA TATTGGGCAG 
CGCCGATTTA ATTTGCAGAA GAATTTTGTT GGGAAAGTGG CACTAATGTT GGGAATTGGA 
ACAGAAGGAC CACACGTGGG TCTCGTTCAA GCCAGTGAAC ACCCCAAAAT AGAATTTTAC 
TTGAAAAACT TTACTTCAGC CAAAGATGTC TTGTTTGCCA TAAAAGAAGT AGGTTTCCGA 
GGGGGTAACT CCAACACAGG AAAAGCCTTG AAGCACACTG CTCAGAAATT CTTTACAGCA 
GACACTGGTG TGAGAAAAGG AATACCAAAA GTGGTGGTAG TGTTTATTGA TGGTTGGCCC 
TCTGATGACA TTGAGGAAGC AGGCATTGTG GCCAGAGAGT TTGGTGTCAA TGTATTTATA 
GTTTCTGTGG CCAAGCCCAT TCCTGAAGAA CTGGGGATGG TTCAAGATGT TGCATTTGTT 
GACAAGGCTG TGTGTCGGAA TAATGGCTTC TTCTCTTATC ACATGCCCAA CTGGTTTGGC 
ACTACAAAAT ATGTGAAGCC TCTGGTGCAG AAGCTCTGTA CGCACGAACA GATGATGTGC 
AGCAAAACCT GCTACAACTC AGTGAACATT GCCTTTCTGA TTGACGGCTC CAGCAGTGTT 
GGAGATAGCA ATTTCCGCCT CATGCTAGAA TTTGTTTCTA ACATAGCGAA GACATTTGAA 
ATCTCAGACA TTGGAGCCAA GATAGCTGCT GTACAGTTCA CTTATGACCA GCGCACCGAG 
TTCAGTTTCA CTGACTATAA TACCAAAGAG AACGTCCTAG CTGTCCTAGC GAACATCCGC 
TACATGAGTG GTGGCACAGC TACTGGTGAT GCCATCGCCT TTACTGTTAG AAATGTATTT 
GGTCCCATAA GGGACAGCCC CAACAAAAAC TTCCTGGTTA TTGTCACAGA TGGGCAGTCC 
TATGATGATG TCCGAGGCCC TGCTGCAGCT GCCCATGATG CAGGTATCAC CATCTTCTCT 
GTTGGTGTGG CTTGGGCACC GCTGGATGAC CTGAGAGATA TGGCCTCTAA ACCCAAAGAG 
TCACACGCTT TCTTTACCAG AGAGTTCACA GGGTTAGAAC CAATTGTCTC TGACGTCATC 
AGAGGCATTT GTAGAGACTT CTTAGAATCC CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031754.1
CDS	284..1942
Translation	MPSSRIPALCLGAWLLLLLLPRFARAEGAVPIPVTCFTRGLDIRKEKADVLCPGGCSLEEFSVFGNIVYASVSSICGAAVHRGVIGTSGGPVRVYSLPGRENYSSVDANGIQSQMLSRWSASFAVTKGKSSTQEATGRAVSTAHPPSGKRLKKTPEKKTGNKDCKADIAFLIDGSFNIGQRRFNLQKNFVGKVALMLGIGTEGPHVGLVQASEHPKIEFYLKNFTSAKDVLFAIKEVGFRGGNSNTGKALKHTAQKFFTADTGVRKGIPKVVVVFIDGWPSDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVAFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQKLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYNTKENVLAVLANIRYMSGGTATGDAIAFTVRNVFGPIRDSPNKNFLVIVTDGQSYDDVRGPAAAAHDAGITIFSVGVAWAPLDDLRDMASKPKESHAFFTREFTGLEPIVSDVIRGICRDFLESQQ

Target ORF information:

RefSeq Version	NM_007728.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus cochlin (Coch), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007728.5

ORF Insert Sequence:

ATGCCCTCGT CCAGGATCCC TGCTCTCTGC CTCGGTGCGT GGCTGCTGCT GCTGCTGCTG 
CCCCGGTTCG CGCGCGCCGA GGGAGCGGTT CCCATTCCTG TCACCTGCTT TACCAGAGGC 
CTGGATATCC GAAAAGAGAA AGCAGATGTT CTCTGCCCAG GAGGCTGCTC TCTTGAGGAA 
TTCTCTGTGT TTGGGAACAT AGTGTATGCG TCAGTGTCCA GCATCTGCGG CGCTGCTGTC 
CATAGGGGAG TGATTGGCAC CTCAGGGGGA CCTGTGCGTG TCTACAGCCT TCCTGGTCGA 
GAGAACTACT CCTCGGTAGA TGCCAACGGC ATCCAGTCTC AGATGCTTTC CCGATGGTCC 
GCGTCCTTCG CTGTGACCAA AGGCAAAAGC AGTACCCAGG AAGCCACAGG ACGGGCAGTG 
TCCACAGCCC ACCCACCTTC AGGTAAAAGA CTAAAGAAGA CACCAGAGAA GAAGACTGGC 
AACAAAGACT GTAAGGCAGA CATTGCATTT CTCATTGATG GAAGCTTCAA TATTGGGCAG 
CGCCGATTTA ATTTGCAGAA GAATTTTGTT GGGAAAGTGG CACTAATGTT GGGAATTGGA 
ACAGAAGGAC CACACGTGGG TCTCGTTCAA GCCAGTGAAC ACCCCAAAAT AGAATTTTAC 
TTGAAAAACT TTACTTCAGC CAAAGATGTC TTGTTTGCCA TAAAAGAAGT AGGTTTCCGA 
GGGGGTAACT CCAACACAGG AAAAGCCTTG AAGCACACTG CTCAGAAATT CTTTACAGCA 
GACACTGGTG TGAGAAAAGG AATACCAAAA GTGGTGGTAG TGTTTATTGA TGGTTGGCCC 
TCTGATGACA TTGAGGAAGC AGGCATTGTG GCCAGAGAGT TTGGTGTCAA TGTATTTATA 
GTTTCTGTGG CCAAGCCCAT TCCTGAAGAA CTGGGGATGG TTCAAGATGT TGCATTTGTT 
GACAAGGCTG TGTGTCGGAA TAATGGCTTC TTCTCTTATC ACATGCCCAA CTGGTTTGGC 
ACTACAAAAT ATGTGAAGCC TCTGGTGCAG AAGCTCTGTA CGCACGAACA GATGATGTGC 
AGCAAAACCT GCTACAACTC AGTGAACATT GCCTTTCTGA TTGACGGCTC CAGCAGTGTT 
GGAGATAGCA ATTTCCGCCT CATGCTAGAA TTTGTTTCTA ACATAGCGAA GACATTTGAA 
ATCTCAGACA TTGGAGCCAA GATAGCTGCT GTACAGTTCA CTTATGACCA GCGCACCGAG 
TTCAGTTTCA CTGACTATAA TACCAAAGAG AACGTCCTAG CTGTCCTAGC GAACATCCGC 
TACATGAGTG GTGGCACAGC TACTGGTGAT GCCATCGCCT TTACTGTTAG AAATGTATTT 
GGTCCCATAA GGGACAGCCC CAACAAAAAC TTCCTGGTTA TTGTCACAGA TGGGCAGTCC 
TATGATGATG TCCGAGGCCC TGCTGCAGCT GCCCATGATG CAGGTATCAC CATCTTCTCT 
GTTGGTGTGG CTTGGGCACC GCTGGATGAC CTGAGAGATA TGGCCTCTAA ACCCAAAGAG 
TCACACGCTT TCTTTACCAG AGAGTTCACA GGGTTAGAAC CAATTGTCTC TGACGTCATC 
AGAGGCATTT GTAGAGACTT CTTAGAATCC CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Coch ( NM_001198835.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001185764.1 Mus musculus cochlin (Coch), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22799
Clone ID Related Accession (Same CDS sequence)	NM_007728.5 , NM_001198835.1
Accession Version	NM_001198835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Mus musculus(house mouse)
Product	cochlin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK137336.1, AF006741.1 and AK028690.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF006741.1, AK171875.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGCCCTCGT CCAGGATCCC TGCTCTCTGC CTCGGTGCGT GGCTGCTGCT GCTGCTGCTG 
CCCCGGTTCG CGCGCGCCGA GGGAGCGGTT CCCATTCCTG TCACCTGCTT TACCAGAGGC 
CTGGATATCC GAAAAGAGAA AGCAGATGTT CTCTGCCCAG GAGGCTGCTC TCTTGAGGAA 
TTCTCTGTGT TTGGGAACAT AGTGTATGCG TCAGTGTCCA GCATCTGCGG CGCTGCTGTC 
CATAGGGGAG TGATTGGCAC CTCAGGGGGA CCTGTGCGTG TCTACAGCCT TCCTGGTCGA 
GAGAACTACT CCTCGGTAGA TGCCAACGGC ATCCAGTCTC AGATGCTTTC CCGATGGTCC 
GCGTCCTTCG CTGTGACCAA AGGCAAAAGC AGTACCCAGG AAGCCACAGG ACGGGCAGTG 
TCCACAGCCC ACCCACCTTC AGGTAAAAGA CTAAAGAAGA CACCAGAGAA GAAGACTGGC 
AACAAAGACT GTAAGGCAGA CATTGCATTT CTCATTGATG GAAGCTTCAA TATTGGGCAG 
CGCCGATTTA ATTTGCAGAA GAATTTTGTT GGGAAAGTGG CACTAATGTT GGGAATTGGA 
ACAGAAGGAC CACACGTGGG TCTCGTTCAA GCCAGTGAAC ACCCCAAAAT AGAATTTTAC 
TTGAAAAACT TTACTTCAGC CAAAGATGTC TTGTTTGCCA TAAAAGAAGT AGGTTTCCGA 
GGGGGTAACT CCAACACAGG AAAAGCCTTG AAGCACACTG CTCAGAAATT CTTTACAGCA 
GACACTGGTG TGAGAAAAGG AATACCAAAA GTGGTGGTAG TGTTTATTGA TGGTTGGCCC 
TCTGATGACA TTGAGGAAGC AGGCATTGTG GCCAGAGAGT TTGGTGTCAA TGTATTTATA 
GTTTCTGTGG CCAAGCCCAT TCCTGAAGAA CTGGGGATGG TTCAAGATGT TGCATTTGTT 
GACAAGGCTG TGTGTCGGAA TAATGGCTTC TTCTCTTATC ACATGCCCAA CTGGTTTGGC 
ACTACAAAAT ATGTGAAGCC TCTGGTGCAG AAGCTCTGTA CGCACGAACA GATGATGTGC 
AGCAAAACCT GCTACAACTC AGTGAACATT GCCTTTCTGA TTGACGGCTC CAGCAGTGTT 
GGAGATAGCA ATTTCCGCCT CATGCTAGAA TTTGTTTCTA ACATAGCGAA GACATTTGAA 
ATCTCAGACA TTGGAGCCAA GATAGCTGCT GTACAGTTCA CTTATGACCA GCGCACCGAG 
TTCAGTTTCA CTGACTATAA TACCAAAGAG AACGTCCTAG CTGTCCTAGC GAACATCCGC 
TACATGAGTG GTGGCACAGC TACTGGTGAT GCCATCGCCT TTACTGTTAG AAATGTATTT 
GGTCCCATAA GGGACAGCCC CAACAAAAAC TTCCTGGTTA TTGTCACAGA TGGGCAGTCC 
TATGATGATG TCCGAGGCCC TGCTGCAGCT GCCCATGATG CAGGTATCAC CATCTTCTCT 
GTTGGTGTGG CTTGGGCACC GCTGGATGAC CTGAGAGATA TGGCCTCTAA ACCCAAAGAG 
TCACACGCTT TCTTTACCAG AGAGTTCACA GGGTTAGAAC CAATTGTCTC TGACGTCATC 
AGAGGCATTT GTAGAGACTT CTTAGAATCC CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185764.1
CDS	85..1743
Translation	MPSSRIPALCLGAWLLLLLLPRFARAEGAVPIPVTCFTRGLDIRKEKADVLCPGGCSLEEFSVFGNIVYASVSSICGAAVHRGVIGTSGGPVRVYSLPGRENYSSVDANGIQSQMLSRWSASFAVTKGKSSTQEATGRAVSTAHPPSGKRLKKTPEKKTGNKDCKADIAFLIDGSFNIGQRRFNLQKNFVGKVALMLGIGTEGPHVGLVQASEHPKIEFYLKNFTSAKDVLFAIKEVGFRGGNSNTGKALKHTAQKFFTADTGVRKGIPKVVVVFIDGWPSDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVAFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQKLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYNTKENVLAVLANIRYMSGGTATGDAIAFTVRNVFGPIRDSPNKNFLVIVTDGQSYDDVRGPAAAAHDAGITIFSVGVAWAPLDDLRDMASKPKESHAFFTREFTGLEPIVSDVIRGICRDFLESQQ

Target ORF information:

RefSeq Version	NM_001198835.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cochlin (Coch), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198835.1

ORF Insert Sequence:

ATGCCCTCGT CCAGGATCCC TGCTCTCTGC CTCGGTGCGT GGCTGCTGCT GCTGCTGCTG 
CCCCGGTTCG CGCGCGCCGA GGGAGCGGTT CCCATTCCTG TCACCTGCTT TACCAGAGGC 
CTGGATATCC GAAAAGAGAA AGCAGATGTT CTCTGCCCAG GAGGCTGCTC TCTTGAGGAA 
TTCTCTGTGT TTGGGAACAT AGTGTATGCG TCAGTGTCCA GCATCTGCGG CGCTGCTGTC 
CATAGGGGAG TGATTGGCAC CTCAGGGGGA CCTGTGCGTG TCTACAGCCT TCCTGGTCGA 
GAGAACTACT CCTCGGTAGA TGCCAACGGC ATCCAGTCTC AGATGCTTTC CCGATGGTCC 
GCGTCCTTCG CTGTGACCAA AGGCAAAAGC AGTACCCAGG AAGCCACAGG ACGGGCAGTG 
TCCACAGCCC ACCCACCTTC AGGTAAAAGA CTAAAGAAGA CACCAGAGAA GAAGACTGGC 
AACAAAGACT GTAAGGCAGA CATTGCATTT CTCATTGATG GAAGCTTCAA TATTGGGCAG 
CGCCGATTTA ATTTGCAGAA GAATTTTGTT GGGAAAGTGG CACTAATGTT GGGAATTGGA 
ACAGAAGGAC CACACGTGGG TCTCGTTCAA GCCAGTGAAC ACCCCAAAAT AGAATTTTAC 
TTGAAAAACT TTACTTCAGC CAAAGATGTC TTGTTTGCCA TAAAAGAAGT AGGTTTCCGA 
GGGGGTAACT CCAACACAGG AAAAGCCTTG AAGCACACTG CTCAGAAATT CTTTACAGCA 
GACACTGGTG TGAGAAAAGG AATACCAAAA GTGGTGGTAG TGTTTATTGA TGGTTGGCCC 
TCTGATGACA TTGAGGAAGC AGGCATTGTG GCCAGAGAGT TTGGTGTCAA TGTATTTATA 
GTTTCTGTGG CCAAGCCCAT TCCTGAAGAA CTGGGGATGG TTCAAGATGT TGCATTTGTT 
GACAAGGCTG TGTGTCGGAA TAATGGCTTC TTCTCTTATC ACATGCCCAA CTGGTTTGGC 
ACTACAAAAT ATGTGAAGCC TCTGGTGCAG AAGCTCTGTA CGCACGAACA GATGATGTGC 
AGCAAAACCT GCTACAACTC AGTGAACATT GCCTTTCTGA TTGACGGCTC CAGCAGTGTT 
GGAGATAGCA ATTTCCGCCT CATGCTAGAA TTTGTTTCTA ACATAGCGAA GACATTTGAA 
ATCTCAGACA TTGGAGCCAA GATAGCTGCT GTACAGTTCA CTTATGACCA GCGCACCGAG 
TTCAGTTTCA CTGACTATAA TACCAAAGAG AACGTCCTAG CTGTCCTAGC GAACATCCGC 
TACATGAGTG GTGGCACAGC TACTGGTGAT GCCATCGCCT TTACTGTTAG AAATGTATTT 
GGTCCCATAA GGGACAGCCC CAACAAAAAC TTCCTGGTTA TTGTCACAGA TGGGCAGTCC 
TATGATGATG TCCGAGGCCC TGCTGCAGCT GCCCATGATG CAGGTATCAC CATCTTCTCT 
GTTGGTGTGG CTTGGGCACC GCTGGATGAC CTGAGAGATA TGGCCTCTAA ACCCAAAGAG 
TCACACGCTT TCTTTACCAG AGAGTTCACA GGGTTAGAAC CAATTGTCTC TGACGTCATC 
AGAGGCATTT GTAGAGACTT CTTAGAATCC CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Coch ( XM_017314943.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170432.1 Mus musculus cochlin (Coch), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu78133
Clone ID Related Accession (Same CDS sequence)	XM_017314943.1 , XM_017314943.2 , XM_017314944.1
Accession Version	XM_017314943.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cochlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGT CCAGGATCCC TGCTCTCTGC CTCGGTGCGT GGCTGCTGCT GCTGCTGCTG 
CCCCGGTTCG CGCGCGCCGA GGGAGCGGGT GAGTTAGTTC CCATTCCTGT CACCTGCTTT 
ACCAGAGGCC TGGATATCCG AAAAGAGAAA GCAGATGTTC TCTGCCCAGG AGGCTGCTCT 
CTTGAGGAAT TCTCTGTGTT TGGGAACATA GTGTATGCGT CAGTGTCCAG CATCTGCGGC 
GCTGCTGTCC ATAGGGGAGT GATTGGCACC TCAGGGGGAC CTGTGCGTGT CTACAGCCTT 
CCTGGTCGAG AGAACTACTC CTCGGTAGAT GCCAACGGCA TCCAGTCTCA GATGCTTTCC 
CGATGGTCCG CGTCCTTCGC TGTGACCAAA GGCAAAAGCA GTACCCAGGA AGCCACAGGA 
CGGGCAGTGT CCACAGCCCA CCCACCTTCA GGTAAAAGAC TAAAGAAGAC ACCAGAGAAG 
AAGACTGGCA ACAAAGACTG TAAGGCAGAC ATTGCATTTC TCATTGATGG AAGCTTCAAT 
ATTGGGCAGC GCCGATTTAA TTTGCAGAAG AATTTTGTTG GGAAAGTGGC ACTAATGTTG 
GGAATTGGAA CAGAAGGACC ACACGTGGGT CTCGTTCAAG CCAGTGAACA CCCCAAAATA 
GAATTTTACT TGAAAAACTT TACTTCAGCC AAAGATGTCT TGTTTGCCAT AAAAGAAGTA 
GGTTTCCGAG GGGGTAACTC CAACACAGGA AAAGCCTTGA AGCACACTGC TCAGAAATTC 
TTTACAGCAG ACACTGGTGT GAGAAAAGGA ATACCAAAAG TGGTGGTAGT GTTTATTGAT 
GGTTGGCCCT CTGATGACAT TGAGGAAGCA GGCATTGTGG CCAGAGAGTT TGGTGTCAAT 
GTATTTATAG TTTCTGTGGC CAAGCCCATT CCTGAAGAAC TGGGGATGGT TCAAGATGTT 
GCATTTGTTG ACAAGGCTGT GTGTCGGAAT AATGGCTTCT TCTCTTATCA CATGCCCAAC 
TGGTTTGGCA CTACAAAATA TGTGAAGCCT CTGGTGCAGA AGCTCTGTAC GCACGAACAG 
ATGATGTGCA GCAAAACCTG CTACAACTCA GTGAACATTG CCTTTCTGAT TGACGGCTCC 
AGCAGTGTTG GAGATAGCAA TTTCCGCCTC ATGCTAGAAT TTGTTTCTAA CATAGCGAAG 
ACATTTGAAA TCTCAGACAT TGGAGCCAAG ATAGCTGCTG TACAGTTCAC TTATGACCAG 
CGCACCGAGT TCAGTTTCAC TGACTATAAT ACCAAAGAGA ACGTCCTAGC TGTCCTAGCG 
AACATCCGCT ACATGAGTGG TGGCACAGCT ACTGGTGATG CCATCGCCTT TACTGTTAGA 
AATGTATTTG GTCCCATAAG GGACAGCCCC AACAAAAACT TCCTGGTTAT TGTCACAGAT 
GGGCAGTCCT ATGATGATGT CCGAGGCCCT GCTGCAGCTG CCCATGATGC AGGTATCACC 
ATCTTCTCTG TTGGTGTGGC TTGGGCACCG CTGGATGACC TGAGAGATAT GGCCTCTAAA 
CCCAAAGAGT CACACGCTTT CTTTACCAGA GAGTTCACAG GGTTAGAACC AATTGTCTCT 
GACGTCATCA GAGGCATTTG TAGAGACTTC TTAGAATCCC AGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170432.1
CDS	153..1820
Translation	MPSSRIPALCLGAWLLLLLLPRFARAEGAGELVPIPVTCFTRGLDIRKEKADVLCPGGCSLEEFSVFGNIVYASVSSICGAAVHRGVIGTSGGPVRVYSLPGRENYSSVDANGIQSQMLSRWSASFAVTKGKSSTQEATGRAVSTAHPPSGKRLKKTPEKKTGNKDCKADIAFLIDGSFNIGQRRFNLQKNFVGKVALMLGIGTEGPHVGLVQASEHPKIEFYLKNFTSAKDVLFAIKEVGFRGGNSNTGKALKHTAQKFFTADTGVRKGIPKVVVVFIDGWPSDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVAFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQKLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYNTKENVLAVLANIRYMSGGTATGDAIAFTVRNVFGPIRDSPNKNFLVIVTDGQSYDDVRGPAAAAHDAGITIFSVGVAWAPLDDLRDMASKPKESHAFFTREFTGLEPIVSDVIRGICRDFLESQQ

Target ORF information:

RefSeq Version	XM_017314943.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cochlin (Coch), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314943.1

ORF Insert Sequence:

ATGCCCTCGT CCAGGATCCC TGCTCTCTGC CTCGGTGCGT GGCTGCTGCT GCTGCTGCTG 
CCCCGGTTCG CGCGCGCCGA GGGAGCGGGT GAGTTAGTTC CCATTCCTGT CACCTGCTTT 
ACCAGAGGCC TGGATATCCG AAAAGAGAAA GCAGATGTTC TCTGCCCAGG AGGCTGCTCT 
CTTGAGGAAT TCTCTGTGTT TGGGAACATA GTGTATGCGT CAGTGTCCAG CATCTGCGGC 
GCTGCTGTCC ATAGGGGAGT GATTGGCACC TCAGGGGGAC CTGTGCGTGT CTACAGCCTT 
CCTGGTCGAG AGAACTACTC CTCGGTAGAT GCCAACGGCA TCCAGTCTCA GATGCTTTCC 
CGATGGTCCG CGTCCTTCGC TGTGACCAAA GGCAAAAGCA GTACCCAGGA AGCCACAGGA 
CGGGCAGTGT CCACAGCCCA CCCACCTTCA GGTAAAAGAC TAAAGAAGAC ACCAGAGAAG 
AAGACTGGCA ACAAAGACTG TAAGGCAGAC ATTGCATTTC TCATTGATGG AAGCTTCAAT 
ATTGGGCAGC GCCGATTTAA TTTGCAGAAG AATTTTGTTG GGAAAGTGGC ACTAATGTTG 
GGAATTGGAA CAGAAGGACC ACACGTGGGT CTCGTTCAAG CCAGTGAACA CCCCAAAATA 
GAATTTTACT TGAAAAACTT TACTTCAGCC AAAGATGTCT TGTTTGCCAT AAAAGAAGTA 
GGTTTCCGAG GGGGTAACTC CAACACAGGA AAAGCCTTGA AGCACACTGC TCAGAAATTC 
TTTACAGCAG ACACTGGTGT GAGAAAAGGA ATACCAAAAG TGGTGGTAGT GTTTATTGAT 
GGTTGGCCCT CTGATGACAT TGAGGAAGCA GGCATTGTGG CCAGAGAGTT TGGTGTCAAT 
GTATTTATAG TTTCTGTGGC CAAGCCCATT CCTGAAGAAC TGGGGATGGT TCAAGATGTT 
GCATTTGTTG ACAAGGCTGT GTGTCGGAAT AATGGCTTCT TCTCTTATCA CATGCCCAAC 
TGGTTTGGCA CTACAAAATA TGTGAAGCCT CTGGTGCAGA AGCTCTGTAC GCACGAACAG 
ATGATGTGCA GCAAAACCTG CTACAACTCA GTGAACATTG CCTTTCTGAT TGACGGCTCC 
AGCAGTGTTG GAGATAGCAA TTTCCGCCTC ATGCTAGAAT TTGTTTCTAA CATAGCGAAG 
ACATTTGAAA TCTCAGACAT TGGAGCCAAG ATAGCTGCTG TACAGTTCAC TTATGACCAG 
CGCACCGAGT TCAGTTTCAC TGACTATAAT ACCAAAGAGA ACGTCCTAGC TGTCCTAGCG 
AACATCCGCT ACATGAGTGG TGGCACAGCT ACTGGTGATG CCATCGCCTT TACTGTTAGA 
AATGTATTTG GTCCCATAAG GGACAGCCCC AACAAAAACT TCCTGGTTAT TGTCACAGAT 
GGGCAGTCCT ATGATGATGT CCGAGGCCCT GCTGCAGCTG CCCATGATGC AGGTATCACC 
ATCTTCTCTG TTGGTGTGGC TTGGGCACCG CTGGATGACC TGAGAGATAT GGCCTCTAAA 
CCCAAAGAGT CACACGCTTT CTTTACCAGA GAGTTCACAG GGTTAGAACC AATTGTCTCT 
GACGTCATCA GAGGCATTTG TAGAGACTTC TTAGAATCCC AGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Coch ( XM_017314943.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170432.1 Mus musculus cochlin (Coch), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu78133
Clone ID Related Accession (Same CDS sequence)	XM_017314943.1 , XM_017314943.2 , XM_017314944.1
Accession Version	XM_017314943.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cochlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314943.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGT CCAGGATCCC TGCTCTCTGC CTCGGTGCGT GGCTGCTGCT GCTGCTGCTG 
CCCCGGTTCG CGCGCGCCGA GGGAGCGGGT GAGTTAGTTC CCATTCCTGT CACCTGCTTT 
ACCAGAGGCC TGGATATCCG AAAAGAGAAA GCAGATGTTC TCTGCCCAGG AGGCTGCTCT 
CTTGAGGAAT TCTCTGTGTT TGGGAACATA GTGTATGCGT CAGTGTCCAG CATCTGCGGC 
GCTGCTGTCC ATAGGGGAGT GATTGGCACC TCAGGGGGAC CTGTGCGTGT CTACAGCCTT 
CCTGGTCGAG AGAACTACTC CTCGGTAGAT GCCAACGGCA TCCAGTCTCA GATGCTTTCC 
CGATGGTCCG CGTCCTTCGC TGTGACCAAA GGCAAAAGCA GTACCCAGGA AGCCACAGGA 
CGGGCAGTGT CCACAGCCCA CCCACCTTCA GGTAAAAGAC TAAAGAAGAC ACCAGAGAAG 
AAGACTGGCA ACAAAGACTG TAAGGCAGAC ATTGCATTTC TCATTGATGG AAGCTTCAAT 
ATTGGGCAGC GCCGATTTAA TTTGCAGAAG AATTTTGTTG GGAAAGTGGC ACTAATGTTG 
GGAATTGGAA CAGAAGGACC ACACGTGGGT CTCGTTCAAG CCAGTGAACA CCCCAAAATA 
GAATTTTACT TGAAAAACTT TACTTCAGCC AAAGATGTCT TGTTTGCCAT AAAAGAAGTA 
GGTTTCCGAG GGGGTAACTC CAACACAGGA AAAGCCTTGA AGCACACTGC TCAGAAATTC 
TTTACAGCAG ACACTGGTGT GAGAAAAGGA ATACCAAAAG TGGTGGTAGT GTTTATTGAT 
GGTTGGCCCT CTGATGACAT TGAGGAAGCA GGCATTGTGG CCAGAGAGTT TGGTGTCAAT 
GTATTTATAG TTTCTGTGGC CAAGCCCATT CCTGAAGAAC TGGGGATGGT TCAAGATGTT 
GCATTTGTTG ACAAGGCTGT GTGTCGGAAT AATGGCTTCT TCTCTTATCA CATGCCCAAC 
TGGTTTGGCA CTACAAAATA TGTGAAGCCT CTGGTGCAGA AGCTCTGTAC GCACGAACAG 
ATGATGTGCA GCAAAACCTG CTACAACTCA GTGAACATTG CCTTTCTGAT TGACGGCTCC 
AGCAGTGTTG GAGATAGCAA TTTCCGCCTC ATGCTAGAAT TTGTTTCTAA CATAGCGAAG 
ACATTTGAAA TCTCAGACAT TGGAGCCAAG ATAGCTGCTG TACAGTTCAC TTATGACCAG 
CGCACCGAGT TCAGTTTCAC TGACTATAAT ACCAAAGAGA ACGTCCTAGC TGTCCTAGCG 
AACATCCGCT ACATGAGTGG TGGCACAGCT ACTGGTGATG CCATCGCCTT TACTGTTAGA 
AATGTATTTG GTCCCATAAG GGACAGCCCC AACAAAAACT TCCTGGTTAT TGTCACAGAT 
GGGCAGTCCT ATGATGATGT CCGAGGCCCT GCTGCAGCTG CCCATGATGC AGGTATCACC 
ATCTTCTCTG TTGGTGTGGC TTGGGCACCG CTGGATGACC TGAGAGATAT GGCCTCTAAA 
CCCAAAGAGT CACACGCTTT CTTTACCAGA GAGTTCACAG GGTTAGAACC AATTGTCTCT 
GACGTCATCA GAGGCATTTG TAGAGACTTC TTAGAATCCC AGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170432.1
CDS	143..1810
Translation	MPSSRIPALCLGAWLLLLLLPRFARAEGAGELVPIPVTCFTRGLDIRKEKADVLCPGGCSLEEFSVFGNIVYASVSSICGAAVHRGVIGTSGGPVRVYSLPGRENYSSVDANGIQSQMLSRWSASFAVTKGKSSTQEATGRAVSTAHPPSGKRLKKTPEKKTGNKDCKADIAFLIDGSFNIGQRRFNLQKNFVGKVALMLGIGTEGPHVGLVQASEHPKIEFYLKNFTSAKDVLFAIKEVGFRGGNSNTGKALKHTAQKFFTADTGVRKGIPKVVVVFIDGWPSDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVAFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQKLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYNTKENVLAVLANIRYMSGGTATGDAIAFTVRNVFGPIRDSPNKNFLVIVTDGQSYDDVRGPAAAAHDAGITIFSVGVAWAPLDDLRDMASKPKESHAFFTREFTGLEPIVSDVIRGICRDFLESQQ

Target ORF information:

RefSeq Version	XM_017314943.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cochlin (Coch), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314943.2

ORF Insert Sequence:

ATGCCCTCGT CCAGGATCCC TGCTCTCTGC CTCGGTGCGT GGCTGCTGCT GCTGCTGCTG 
CCCCGGTTCG CGCGCGCCGA GGGAGCGGGT GAGTTAGTTC CCATTCCTGT CACCTGCTTT 
ACCAGAGGCC TGGATATCCG AAAAGAGAAA GCAGATGTTC TCTGCCCAGG AGGCTGCTCT 
CTTGAGGAAT TCTCTGTGTT TGGGAACATA GTGTATGCGT CAGTGTCCAG CATCTGCGGC 
GCTGCTGTCC ATAGGGGAGT GATTGGCACC TCAGGGGGAC CTGTGCGTGT CTACAGCCTT 
CCTGGTCGAG AGAACTACTC CTCGGTAGAT GCCAACGGCA TCCAGTCTCA GATGCTTTCC 
CGATGGTCCG CGTCCTTCGC TGTGACCAAA GGCAAAAGCA GTACCCAGGA AGCCACAGGA 
CGGGCAGTGT CCACAGCCCA CCCACCTTCA GGTAAAAGAC TAAAGAAGAC ACCAGAGAAG 
AAGACTGGCA ACAAAGACTG TAAGGCAGAC ATTGCATTTC TCATTGATGG AAGCTTCAAT 
ATTGGGCAGC GCCGATTTAA TTTGCAGAAG AATTTTGTTG GGAAAGTGGC ACTAATGTTG 
GGAATTGGAA CAGAAGGACC ACACGTGGGT CTCGTTCAAG CCAGTGAACA CCCCAAAATA 
GAATTTTACT TGAAAAACTT TACTTCAGCC AAAGATGTCT TGTTTGCCAT AAAAGAAGTA 
GGTTTCCGAG GGGGTAACTC CAACACAGGA AAAGCCTTGA AGCACACTGC TCAGAAATTC 
TTTACAGCAG ACACTGGTGT GAGAAAAGGA ATACCAAAAG TGGTGGTAGT GTTTATTGAT 
GGTTGGCCCT CTGATGACAT TGAGGAAGCA GGCATTGTGG CCAGAGAGTT TGGTGTCAAT 
GTATTTATAG TTTCTGTGGC CAAGCCCATT CCTGAAGAAC TGGGGATGGT TCAAGATGTT 
GCATTTGTTG ACAAGGCTGT GTGTCGGAAT AATGGCTTCT TCTCTTATCA CATGCCCAAC 
TGGTTTGGCA CTACAAAATA TGTGAAGCCT CTGGTGCAGA AGCTCTGTAC GCACGAACAG 
ATGATGTGCA GCAAAACCTG CTACAACTCA GTGAACATTG CCTTTCTGAT TGACGGCTCC 
AGCAGTGTTG GAGATAGCAA TTTCCGCCTC ATGCTAGAAT TTGTTTCTAA CATAGCGAAG 
ACATTTGAAA TCTCAGACAT TGGAGCCAAG ATAGCTGCTG TACAGTTCAC TTATGACCAG 
CGCACCGAGT TCAGTTTCAC TGACTATAAT ACCAAAGAGA ACGTCCTAGC TGTCCTAGCG 
AACATCCGCT ACATGAGTGG TGGCACAGCT ACTGGTGATG CCATCGCCTT TACTGTTAGA 
AATGTATTTG GTCCCATAAG GGACAGCCCC AACAAAAACT TCCTGGTTAT TGTCACAGAT 
GGGCAGTCCT ATGATGATGT CCGAGGCCCT GCTGCAGCTG CCCATGATGC AGGTATCACC 
ATCTTCTCTG TTGGTGTGGC TTGGGCACCG CTGGATGACC TGAGAGATAT GGCCTCTAAA 
CCCAAAGAGT CACACGCTTT CTTTACCAGA GAGTTCACAG GGTTAGAACC AATTGTCTCT 
GACGTCATCA GAGGCATTTG TAGAGACTTC TTAGAATCCC AGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Coch ( XM_017314944.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170433.1 Mus musculus cochlin (Coch), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu78133
Clone ID Related Accession (Same CDS sequence)	XM_017314943.1 , XM_017314943.2 , XM_017314944.1
Accession Version	XM_017314944.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cochlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGT CCAGGATCCC TGCTCTCTGC CTCGGTGCGT GGCTGCTGCT GCTGCTGCTG 
CCCCGGTTCG CGCGCGCCGA GGGAGCGGGT GAGTTAGTTC CCATTCCTGT CACCTGCTTT 
ACCAGAGGCC TGGATATCCG AAAAGAGAAA GCAGATGTTC TCTGCCCAGG AGGCTGCTCT 
CTTGAGGAAT TCTCTGTGTT TGGGAACATA GTGTATGCGT CAGTGTCCAG CATCTGCGGC 
GCTGCTGTCC ATAGGGGAGT GATTGGCACC TCAGGGGGAC CTGTGCGTGT CTACAGCCTT 
CCTGGTCGAG AGAACTACTC CTCGGTAGAT GCCAACGGCA TCCAGTCTCA GATGCTTTCC 
CGATGGTCCG CGTCCTTCGC TGTGACCAAA GGCAAAAGCA GTACCCAGGA AGCCACAGGA 
CGGGCAGTGT CCACAGCCCA CCCACCTTCA GGTAAAAGAC TAAAGAAGAC ACCAGAGAAG 
AAGACTGGCA ACAAAGACTG TAAGGCAGAC ATTGCATTTC TCATTGATGG AAGCTTCAAT 
ATTGGGCAGC GCCGATTTAA TTTGCAGAAG AATTTTGTTG GGAAAGTGGC ACTAATGTTG 
GGAATTGGAA CAGAAGGACC ACACGTGGGT CTCGTTCAAG CCAGTGAACA CCCCAAAATA 
GAATTTTACT TGAAAAACTT TACTTCAGCC AAAGATGTCT TGTTTGCCAT AAAAGAAGTA 
GGTTTCCGAG GGGGTAACTC CAACACAGGA AAAGCCTTGA AGCACACTGC TCAGAAATTC 
TTTACAGCAG ACACTGGTGT GAGAAAAGGA ATACCAAAAG TGGTGGTAGT GTTTATTGAT 
GGTTGGCCCT CTGATGACAT TGAGGAAGCA GGCATTGTGG CCAGAGAGTT TGGTGTCAAT 
GTATTTATAG TTTCTGTGGC CAAGCCCATT CCTGAAGAAC TGGGGATGGT TCAAGATGTT 
GCATTTGTTG ACAAGGCTGT GTGTCGGAAT AATGGCTTCT TCTCTTATCA CATGCCCAAC 
TGGTTTGGCA CTACAAAATA TGTGAAGCCT CTGGTGCAGA AGCTCTGTAC GCACGAACAG 
ATGATGTGCA GCAAAACCTG CTACAACTCA GTGAACATTG CCTTTCTGAT TGACGGCTCC 
AGCAGTGTTG GAGATAGCAA TTTCCGCCTC ATGCTAGAAT TTGTTTCTAA CATAGCGAAG 
ACATTTGAAA TCTCAGACAT TGGAGCCAAG ATAGCTGCTG TACAGTTCAC TTATGACCAG 
CGCACCGAGT TCAGTTTCAC TGACTATAAT ACCAAAGAGA ACGTCCTAGC TGTCCTAGCG 
AACATCCGCT ACATGAGTGG TGGCACAGCT ACTGGTGATG CCATCGCCTT TACTGTTAGA 
AATGTATTTG GTCCCATAAG GGACAGCCCC AACAAAAACT TCCTGGTTAT TGTCACAGAT 
GGGCAGTCCT ATGATGATGT CCGAGGCCCT GCTGCAGCTG CCCATGATGC AGGTATCACC 
ATCTTCTCTG TTGGTGTGGC TTGGGCACCG CTGGATGACC TGAGAGATAT GGCCTCTAAA 
CCCAAAGAGT CACACGCTTT CTTTACCAGA GAGTTCACAG GGTTAGAACC AATTGTCTCT 
GACGTCATCA GAGGCATTTG TAGAGACTTC TTAGAATCCC AGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170433.1
CDS	352..2019
Translation	MPSSRIPALCLGAWLLLLLLPRFARAEGAGELVPIPVTCFTRGLDIRKEKADVLCPGGCSLEEFSVFGNIVYASVSSICGAAVHRGVIGTSGGPVRVYSLPGRENYSSVDANGIQSQMLSRWSASFAVTKGKSSTQEATGRAVSTAHPPSGKRLKKTPEKKTGNKDCKADIAFLIDGSFNIGQRRFNLQKNFVGKVALMLGIGTEGPHVGLVQASEHPKIEFYLKNFTSAKDVLFAIKEVGFRGGNSNTGKALKHTAQKFFTADTGVRKGIPKVVVVFIDGWPSDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVAFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQKLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYNTKENVLAVLANIRYMSGGTATGDAIAFTVRNVFGPIRDSPNKNFLVIVTDGQSYDDVRGPAAAAHDAGITIFSVGVAWAPLDDLRDMASKPKESHAFFTREFTGLEPIVSDVIRGICRDFLESQQ

Target ORF information:

RefSeq Version	XM_017314944.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cochlin (Coch), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314944.1

ORF Insert Sequence:

ATGCCCTCGT CCAGGATCCC TGCTCTCTGC CTCGGTGCGT GGCTGCTGCT GCTGCTGCTG 
CCCCGGTTCG CGCGCGCCGA GGGAGCGGGT GAGTTAGTTC CCATTCCTGT CACCTGCTTT 
ACCAGAGGCC TGGATATCCG AAAAGAGAAA GCAGATGTTC TCTGCCCAGG AGGCTGCTCT 
CTTGAGGAAT TCTCTGTGTT TGGGAACATA GTGTATGCGT CAGTGTCCAG CATCTGCGGC 
GCTGCTGTCC ATAGGGGAGT GATTGGCACC TCAGGGGGAC CTGTGCGTGT CTACAGCCTT 
CCTGGTCGAG AGAACTACTC CTCGGTAGAT GCCAACGGCA TCCAGTCTCA GATGCTTTCC 
CGATGGTCCG CGTCCTTCGC TGTGACCAAA GGCAAAAGCA GTACCCAGGA AGCCACAGGA 
CGGGCAGTGT CCACAGCCCA CCCACCTTCA GGTAAAAGAC TAAAGAAGAC ACCAGAGAAG 
AAGACTGGCA ACAAAGACTG TAAGGCAGAC ATTGCATTTC TCATTGATGG AAGCTTCAAT 
ATTGGGCAGC GCCGATTTAA TTTGCAGAAG AATTTTGTTG GGAAAGTGGC ACTAATGTTG 
GGAATTGGAA CAGAAGGACC ACACGTGGGT CTCGTTCAAG CCAGTGAACA CCCCAAAATA 
GAATTTTACT TGAAAAACTT TACTTCAGCC AAAGATGTCT TGTTTGCCAT AAAAGAAGTA 
GGTTTCCGAG GGGGTAACTC CAACACAGGA AAAGCCTTGA AGCACACTGC TCAGAAATTC 
TTTACAGCAG ACACTGGTGT GAGAAAAGGA ATACCAAAAG TGGTGGTAGT GTTTATTGAT 
GGTTGGCCCT CTGATGACAT TGAGGAAGCA GGCATTGTGG CCAGAGAGTT TGGTGTCAAT 
GTATTTATAG TTTCTGTGGC CAAGCCCATT CCTGAAGAAC TGGGGATGGT TCAAGATGTT 
GCATTTGTTG ACAAGGCTGT GTGTCGGAAT AATGGCTTCT TCTCTTATCA CATGCCCAAC 
TGGTTTGGCA CTACAAAATA TGTGAAGCCT CTGGTGCAGA AGCTCTGTAC GCACGAACAG 
ATGATGTGCA GCAAAACCTG CTACAACTCA GTGAACATTG CCTTTCTGAT TGACGGCTCC 
AGCAGTGTTG GAGATAGCAA TTTCCGCCTC ATGCTAGAAT TTGTTTCTAA CATAGCGAAG 
ACATTTGAAA TCTCAGACAT TGGAGCCAAG ATAGCTGCTG TACAGTTCAC TTATGACCAG 
CGCACCGAGT TCAGTTTCAC TGACTATAAT ACCAAAGAGA ACGTCCTAGC TGTCCTAGCG 
AACATCCGCT ACATGAGTGG TGGCACAGCT ACTGGTGATG CCATCGCCTT TACTGTTAGA 
AATGTATTTG GTCCCATAAG GGACAGCCCC AACAAAAACT TCCTGGTTAT TGTCACAGAT 
GGGCAGTCCT ATGATGATGT CCGAGGCCCT GCTGCAGCTG CCCATGATGC AGGTATCACC 
ATCTTCTCTG TTGGTGTGGC TTGGGCACCG CTGGATGACC TGAGAGATAT GGCCTCTAAA 
CCCAAAGAGT CACACGCTTT CTTTACCAGA GAGTTCACAG GGTTAGAACC AATTGTCTCT 
GACGTCATCA GAGGCATTTG TAGAGACTTC TTAGAATCCC AGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cochlin in normal middle ear and abnormal middle ear deposits in DFNA9 and Coch (G88E/G88E) mice.
Journal of the Association for Research in Otolaryngology : JARO15(6)961-74(2014 Dec)
Robertson NG,O'Malley JT,Ong CA,Giersch AB,Shen J,Stankovic KM,Morton CC

Resistance to noise-induced hearing loss in 129S6 and MOLF mice: identification of independent, overlapping, and interacting chromosomal regions.
Journal of the Association for Research in Otolaryngology : JARO15(5)721-38(2014 Oct)
Street VA,Kujawa SG,Manichaikul A,Broman KW,Kallman JC,Shilling DJ,Iwata AJ,Robinson LC,Robbins CA,Li J,Liberman MC,Tempel BL

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Canonical Wnt signaling regulates the proliferative expansion and differentiation of fibrocytes in the murine inner ear.
Developmental biology391(1)54-65(2014 Jul)
Bohnenpoll T,Trowe MO,Wojahn I,Taketo MM,Petry M,Kispert A

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Coch cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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オンラインフォーム