Clk2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clk2
Entrez Gene ID	12748
Full Name	CDC-like kinase 2
Synonyms	AU041688
General protein information	Preferred Names CDC-like kinase 2 Names dual specificity protein kinase CLK2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001163432.2	NP_001156904.1	dual specificity protein kinase CLK2 isoform 2
NM_007712.4	NP_031738.2	dual specificity protein kinase CLK2 isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CLK2	XP_547549.4
Gallus gallus (chicken)	CLK2	NP_001026497.1
Pan troglodytes (chimpanzee)	CLK2	XP_513855.3
Mus musculus (house mouse)	Clk2	NP_031738.2
Danio rerio (zebrafish)	clk2a	NP_001038344.1
Drosophila melanogaster (fruit fly)	Doa	NP_788758.3
Bos taurus (cattle)	CLK2	NP_001193193.1
Xenopus tropicalis (tropical clawed frog)	clk2	NP_001011361.1
Homo sapiens (human)	CLK2	NP_003984.2
Rattus norvegicus (Norway rat)	Clk2	NP_001014276.1
Arabidopsis thaliana (thale cress)	FC2	NP_194205.1
Caenorhabditis elegans (roundworm)	tag-172	NP_741927.1

Gene Ontology
Bibliography

Related articles in PubMed

Adipose Tissue CLK2 Promotes Energy Expenditure during High-Fat Diet Intermittent Fasting.
Hatting M, Rines AK, Luo C, Tabata M, Sharabi K, Hall JA, Verdeguer F, Trautwein C, Puigserver P
Cell metabolism25(2)428-437(2017 Feb)

CLK2 inhibition ameliorates autistic features associated with SHANK3 deficiency.
Bidinosti M, Botta P, Kr?ttner S, Proenca CC, Stoehr N, Bernhard M, Fruh I, Mueller M, Bonenfant D, Voshol H, Carbone W, Neal SJ, McTighe SM, Roma G, Dolmetsch RE, Porter JA, Caroni P, Bouwmeester T, L?thi A, Galimberti I
Science (New York, N.Y.)351(6278)1199-203(2016 Mar)

Cdc2-like kinase 2 suppresses hepatic fatty acid oxidation and ketogenesis through disruption of the PGC-1? and MED1 complex.
Tabata M, Rodgers JT, Hall JA, Lee Y, Jedrychowski MP, Gygi SP, Puigserver P
Diabetes63(5)1519-32(2014 May)

The highly conserved LAMMER/CLK2 protein kinases prevent germ cell overproliferation in Drosophila.
Zhao S, Chen D, Geng Q, Wang Z
Developmental biology376(2)163-70(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CLK2 is a regulatory component of diet-induced thermogenesis in brown adipose tissue.
Title: Adipose Tissue CLK2 Promotes Energy Expenditure during High-Fat Diet Intermittent Fasting.

Liver-specific Clk2 knockout mice fed a high-fat diet exhibit increased fasting levels of blood ketone bodies, reduced respiratory exchange ratio, and increased gene expression of fatty acid oxidation and ketogenic pathways.
Title: Cdc2-like kinase 2 suppresses hepatic fatty acid oxidation and ketogenesis through disruption of the PGC-1? and MED1 complex.

LAMMER/Cdc2-like kinase, represented by Doa and its mammalian homolog CLK2, is a critical and conserved component in the regulatory program of the mitosis-to-meiosis switch
Title: The highly conserved LAMMER/CLK2 protein kinases prevent germ cell overproliferation in Drosophila.

Cdc2-like kinase 2 (Clk2) directly phosphorylates the SR domain on PGC-1alpha, resulting in repression of gluconeogenic gene expression and hepatic glucose output.
Title: Cdc2-like kinase 2 is an insulin-regulated suppressor of hepatic gluconeogenesis.

The following Clk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00659	NM_007712.4 Latest version!	Mus musculus CDC-like kinase 2 (Clk2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$293.30 $419.00
OMu09009	NM_001163432.2 Latest version!	Mus musculus CDC-like kinase 2 (Clk2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$321.30 $459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clk2 ( NM_007712.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031738.2 Mus musculus CDC-like kinase 2 (Clk2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00659
Clone ID Related Accession (Same CDS sequence)	NM_007712.4
Accession Version	NM_007712.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-13
Organism	Mus musculus(house mouse)
Product	dual specificity protein kinase CLK2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY247622.1, BY021174.1, BY738871.1, DT924403.1, CN538691.1, CD740936.1 and AC161600.6. On Jun 20, 2015 this sequence version replaced NM_007712.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF033564.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCATC CCCGAAGGTA CCATTCCTCA GAGCGAGGTA GCCGGGGGAG TTACCACGAA 
CACTATCAGA GCCGAAAGCA TAAGCGAAGA AGAAGTCGCT CCTGGTCAAG TAGCAGTGAC 
CGGACAAGGC GGCGGCGGAG GGAGGACAGC TACCACGTTC GGTCCCGAAG CAGCTATGAT 
GACCATTCGT CCGATCGGCG GCTGTACGAT CGGCGGTACT GTGGCAGCTA CAGGCGCAAT 
GACTACAGCC GGGACAGAGG GGAGGCTTAC TACGACACAG ACTTTCGGCA GTCCTATGAA 
TACCATCGAG AGAACAGCAG TTACCGAAGC CAGCGCAGCA GCCGAAGGAA ACACAGAAGG 
CGGAGGAGAC GGAGCCGGAC ATTCAGCCGC TCATCTTCAC ACAGCAGCCG GAGAGCCAAG 
AGTGTAGAGG ACGACGCTGA GGGCCACCTC ATCTACCACG TCGGGGACTG GCTACAAGAG 
CGATATGAAA TTGTAAGCAC CTTAGGAGAA GGGACCTTCG GCCGAGTTGT GCAGTGTGTG 
GACCATCGCA GGGGCGGAAC ACAAGTTGCC CTGAAGATCA TTAAGAATGT GGAGAAGTAC 
AAGGAAGCAG CCCGACTAGA AATCAACGTG CTGGAGAAAA TCAATGAGAA AGATCCTGAC 
AACAAGAACC TCTGTGTCCA GATGTTTGAC TGGTTTGACT ACCATGGCCA CATGTGTATC 
TCCTTTGAGC TTCTGGGCCT TAGCACCTTC GATTTCCTCA AAGACAACAA CTACCTGCCC 
TACCCCATCC ACCAAGTGCG CCACATGGCC TTCCAGCTCT GCCAGGCCGT CAAGTTCCTC 
CATGATAACA AGTTGACACA TACGGACCTC AAACCTGAAA ATATTCTGTT TGTGAATTCA 
GACTACGAAC TCACCTACAA CCTAGAGAAG AAGCGAGATG AGCGCAGTGT AAAGAGCACA 
GCCGTGCGGG TGGTGGACTT CGGCAGTGCC ACCTTTGACC ACGAACACCA TAGCACCATT 
GTCTCCACTC GCCATTACCG AGCCCCCGAG GTCATCCTGG AGTTGGGCTG GTCACAGCCA 
TGCGATGTAT GGAGCATAGG CTGCATCATC TTTGAGTACT ACGTTGGCTT CACCCTCTTC 
CAGACCCATG ACAACAGAGA GCATCTAGCC ATGATGGAAA GGATCCTGGG TCCTGTCCCT 
TCTCGGATGA TCAGAAAGAC AAGAAAACAG AAATATTTTT ATCGGGGTCG CCTGGATTGG 
GATGAGAACA CCTCAGCCGG GCGCTACGTT CGTGAGAACT GCAAACCTCT GCGGCGGTAT 
CTGACCTCAG AGGCAGAGGA CCACCACCAG CTCTTCGATC TGATTGAAAA TATGCTAGAG 
TATGAGCCTG CTAAGCGGCT GACCTTAGGT GAAGCCCTTC AGCATCCTTT CTTCGCCTGC 
CTTCGGACTG AGCCACCCAA CACCAAGTTG TGGGACTCCA GTCGGGATAT CAGTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031738.2
CDS	313..1812
Translation	MPHPRRYHSSERGSRGSYHEHYQSRKHKRRRSRSWSSSSDRTRRRRREDSYHVRSRSSYDDHSSDRRLYDRRYCGSYRRNDYSRDRGEAYYDTDFRQSYEYHRENSSYRSQRSSRRKHRRRRRRSRTFSRSSSHSSRRAKSVEDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGTQVALKIIKNVEKYKEAARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYPIHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTYNLEKKRDERSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAMMERILGPVPSRMIRKTRKQKYFYRGRLDWDENTSAGRYVRENCKPLRRYLTSEAEDHHQLFDLIENMLEYEPAKRLTLGEALQHPFFACLRTEPPNTKLWDSSRDISR

Target ORF information:

RefSeq Version	NM_007712.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC-like kinase 2 (Clk2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007712.4

ORF Insert Sequence:

ATGCCCCATC CCCGAAGGTA CCATTCCTCA GAGCGAGGTA GCCGGGGGAG TTACCACGAA 
CACTATCAGA GCCGAAAGCA TAAGCGAAGA AGAAGTCGCT CCTGGTCAAG TAGCAGTGAC 
CGGACAAGGC GGCGGCGGAG GGAGGACAGC TACCACGTTC GGTCCCGAAG CAGCTATGAT 
GACCATTCGT CCGATCGGCG GCTGTACGAT CGGCGGTACT GTGGCAGCTA CAGGCGCAAT 
GACTACAGCC GGGACAGAGG GGAGGCTTAC TACGACACAG ACTTTCGGCA GTCCTATGAA 
TACCATCGAG AGAACAGCAG TTACCGAAGC CAGCGCAGCA GCCGAAGGAA ACACAGAAGG 
CGGAGGAGAC GGAGCCGGAC ATTCAGCCGC TCATCTTCAC ACAGCAGCCG GAGAGCCAAG 
AGTGTAGAGG ACGACGCTGA GGGCCACCTC ATCTACCACG TCGGGGACTG GCTACAAGAG 
CGATATGAAA TTGTAAGCAC CTTAGGAGAA GGGACCTTCG GCCGAGTTGT GCAGTGTGTG 
GACCATCGCA GGGGCGGAAC ACAAGTTGCC CTGAAGATCA TTAAGAATGT GGAGAAGTAC 
AAGGAAGCAG CCCGACTAGA AATCAACGTG CTGGAGAAAA TCAATGAGAA AGATCCTGAC 
AACAAGAACC TCTGTGTCCA GATGTTTGAC TGGTTTGACT ACCATGGCCA CATGTGTATC 
TCCTTTGAGC TTCTGGGCCT TAGCACCTTC GATTTCCTCA AAGACAACAA CTACCTGCCC 
TACCCCATCC ACCAAGTGCG CCACATGGCC TTCCAGCTCT GCCAGGCCGT CAAGTTCCTC 
CATGATAACA AGTTGACACA TACGGACCTC AAACCTGAAA ATATTCTGTT TGTGAATTCA 
GACTACGAAC TCACCTACAA CCTAGAGAAG AAGCGAGATG AGCGCAGTGT AAAGAGCACA 
GCCGTGCGGG TGGTGGACTT CGGCAGTGCC ACCTTTGACC ACGAACACCA TAGCACCATT 
GTCTCCACTC GCCATTACCG AGCCCCCGAG GTCATCCTGG AGTTGGGCTG GTCACAGCCA 
TGCGATGTAT GGAGCATAGG CTGCATCATC TTTGAGTACT ACGTTGGCTT CACCCTCTTC 
CAGACCCATG ACAACAGAGA GCATCTAGCC ATGATGGAAA GGATCCTGGG TCCTGTCCCT 
TCTCGGATGA TCAGAAAGAC AAGAAAACAG AAATATTTTT ATCGGGGTCG CCTGGATTGG 
GATGAGAACA CCTCAGCCGG GCGCTACGTT CGTGAGAACT GCAAACCTCT GCGGCGGTAT 
CTGACCTCAG AGGCAGAGGA CCACCACCAG CTCTTCGATC TGATTGAAAA TATGCTAGAG 
TATGAGCCTG CTAAGCGGCT GACCTTAGGT GAAGCCCTTC AGCATCCTTT CTTCGCCTGC 
CTTCGGACTG AGCCACCCAA CACCAAGTTG TGGGACTCCA GTCGGGATAT CAGTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clk2 ( NM_001163432.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156904.1 Mus musculus CDC-like kinase 2 (Clk2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu09009
Clone ID Related Accession (Same CDS sequence)	NM_001163432.2
Accession Version	NM_001163432.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-13
Organism	Mus musculus(house mouse)
Product	dual specificity protein kinase CLK2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY247622.1, BY021174.1, BY738871.1, DT924403.1, CN538691.1, CD740936.1 and AC161600.6. On Jun 20, 2015 this sequence version replaced NM_001163432.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015080.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCATC CCCGAAGGTA CCATTCCTCA GAGCGAGGTA GCCGGGGGAG TTACCACGAA 
CACTATCAGA GCCGAAAGCA TAAGCGAAGA AGAAGTCGCT CCTGGTCAAG TAGCAGTGAC 
CGGACAAGGC GGCGGCGGAG GGAGGACAGC TACCACGTTC GGTCCCGAAG CTATGATGAC 
CATTCGTCCG ATCGGCGGCT GTACGATCGG CGGTACTGTG GCAGCTACAG GCGCAATGAC 
TACAGCCGGG ACAGAGGGGA GGCTTACTAC GACACAGACT TTCGGCAGTC CTATGAATAC 
CATCGAGAGA ACAGCAGTTA CCGAAGCCAG CGCAGCAGCC GAAGGAAACA CAGAAGGCGG 
AGGAGACGGA GCCGGACATT CAGCCGCTCA TCTTCACACA GCAGCCGGAG AGCCAAGAGT 
GTAGAGGACG ACGCTGAGGG CCACCTCATC TACCACGTCG GGGACTGGCT ACAAGAGCGA 
TATGAAATTG TAAGCACCTT AGGAGAAGGG ACCTTCGGCC GAGTTGTGCA GTGTGTGGAC 
CATCGCAGGG GCGGAACACA AGTTGCCCTG AAGATCATTA AGAATGTGGA GAAGTACAAG 
GAAGCAGCCC GACTAGAAAT CAACGTGCTG GAGAAAATCA ATGAGAAAGA TCCTGACAAC 
AAGAACCTCT GTGTCCAGAT GTTTGACTGG TTTGACTACC ATGGCCACAT GTGTATCTCC 
TTTGAGCTTC TGGGCCTTAG CACCTTCGAT TTCCTCAAAG ACAACAACTA CCTGCCCTAC 
CCCATCCACC AAGTGCGCCA CATGGCCTTC CAGCTCTGCC AGGCCGTCAA GTTCCTCCAT 
GATAACAAGT TGACACATAC GGACCTCAAA CCTGAAAATA TTCTGTTTGT GAATTCAGAC 
TACGAACTCA CCTACAACCT AGAGAAGAAG CGAGATGAGC GCAGTGTAAA GAGCACAGCC 
GTGCGGGTGG TGGACTTCGG CAGTGCCACC TTTGACCACG AACACCATAG CACCATTGTC 
TCCACTCGCC ATTACCGAGC CCCCGAGGTC ATCCTGGAGT TGGGCTGGTC ACAGCCATGC 
GATGTATGGA GCATAGGCTG CATCATCTTT GAGTACTACG TTGGCTTCAC CCTCTTCCAG 
ACCCATGACA ACAGAGAGCA TCTAGCCATG ATGGAAAGGA TCCTGGGTCC TGTCCCTTCT 
CGGATGATCA GAAAGACAAG AAAACAGAAA TATTTTTATC GGGGTCGCCT GGATTGGGAT 
GAGAACACCT CAGCCGGGCG CTACGTTCGT GAGAACTGCA AACCTCTGCG GCGGTATCTG 
ACCTCAGAGG CAGAGGACCA CCACCAGCTC TTCGATCTGA TTGAAAATAT GCTAGAGTAT 
GAGCCTGCTA AGCGGCTGAC CTTAGGTGAA GCCCTTCAGC ATCCTTTCTT CGCCTGCCTT 
CGGACTGAGC CACCCAACAC CAAGTTGTGG GACTCCAGTC GGGATATCAG TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156904.1
CDS	313..1809
Translation	MPHPRRYHSSERGSRGSYHEHYQSRKHKRRRSRSWSSSSDRTRRRRREDSYHVRSRSYDDHSSDRRLYDRRYCGSYRRNDYSRDRGEAYYDTDFRQSYEYHRENSSYRSQRSSRRKHRRRRRRSRTFSRSSSHSSRRAKSVEDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGTQVALKIIKNVEKYKEAARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYPIHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTYNLEKKRDERSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAMMERILGPVPSRMIRKTRKQKYFYRGRLDWDENTSAGRYVRENCKPLRRYLTSEAEDHHQLFDLIENMLEYEPAKRLTLGEALQHPFFACLRTEPPNTKLWDSSRDISR

Target ORF information:

RefSeq Version	NM_001163432.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDC-like kinase 2 (Clk2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163432.2

ORF Insert Sequence:

ATGCCCCATC CCCGAAGGTA CCATTCCTCA GAGCGAGGTA GCCGGGGGAG TTACCACGAA 
CACTATCAGA GCCGAAAGCA TAAGCGAAGA AGAAGTCGCT CCTGGTCAAG TAGCAGTGAC 
CGGACAAGGC GGCGGCGGAG GGAGGACAGC TACCACGTTC GGTCCCGAAG CTATGATGAC 
CATTCGTCCG ATCGGCGGCT GTACGATCGG CGGTACTGTG GCAGCTACAG GCGCAATGAC 
TACAGCCGGG ACAGAGGGGA GGCTTACTAC GACACAGACT TTCGGCAGTC CTATGAATAC 
CATCGAGAGA ACAGCAGTTA CCGAAGCCAG CGCAGCAGCC GAAGGAAACA CAGAAGGCGG 
AGGAGACGGA GCCGGACATT CAGCCGCTCA TCTTCACACA GCAGCCGGAG AGCCAAGAGT 
GTAGAGGACG ACGCTGAGGG CCACCTCATC TACCACGTCG GGGACTGGCT ACAAGAGCGA 
TATGAAATTG TAAGCACCTT AGGAGAAGGG ACCTTCGGCC GAGTTGTGCA GTGTGTGGAC 
CATCGCAGGG GCGGAACACA AGTTGCCCTG AAGATCATTA AGAATGTGGA GAAGTACAAG 
GAAGCAGCCC GACTAGAAAT CAACGTGCTG GAGAAAATCA ATGAGAAAGA TCCTGACAAC 
AAGAACCTCT GTGTCCAGAT GTTTGACTGG TTTGACTACC ATGGCCACAT GTGTATCTCC 
TTTGAGCTTC TGGGCCTTAG CACCTTCGAT TTCCTCAAAG ACAACAACTA CCTGCCCTAC 
CCCATCCACC AAGTGCGCCA CATGGCCTTC CAGCTCTGCC AGGCCGTCAA GTTCCTCCAT 
GATAACAAGT TGACACATAC GGACCTCAAA CCTGAAAATA TTCTGTTTGT GAATTCAGAC 
TACGAACTCA CCTACAACCT AGAGAAGAAG CGAGATGAGC GCAGTGTAAA GAGCACAGCC 
GTGCGGGTGG TGGACTTCGG CAGTGCCACC TTTGACCACG AACACCATAG CACCATTGTC 
TCCACTCGCC ATTACCGAGC CCCCGAGGTC ATCCTGGAGT TGGGCTGGTC ACAGCCATGC 
GATGTATGGA GCATAGGCTG CATCATCTTT GAGTACTACG TTGGCTTCAC CCTCTTCCAG 
ACCCATGACA ACAGAGAGCA TCTAGCCATG ATGGAAAGGA TCCTGGGTCC TGTCCCTTCT 
CGGATGATCA GAAAGACAAG AAAACAGAAA TATTTTTATC GGGGTCGCCT GGATTGGGAT 
GAGAACACCT CAGCCGGGCG CTACGTTCGT GAGAACTGCA AACCTCTGCG GCGGTATCTG 
ACCTCAGAGG CAGAGGACCA CCACCAGCTC TTCGATCTGA TTGAAAATAT GCTAGAGTAT 
GAGCCTGCTA AGCGGCTGAC CTTAGGTGAA GCCCTTCAGC ATCCTTTCTT CGCCTGCCTT 
CGGACTGAGC CACCCAACAC CAAGTTGTGG GACTCCAGTC GGGATATCAG TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Adipose Tissue CLK2 Promotes Energy Expenditure during High-Fat Diet Intermittent Fasting.
Cell metabolism25(2)428-437(2017 Feb)
Hatting M,Rines AK,Luo C,Tabata M,Sharabi K,Hall JA,Verdeguer F,Trautwein C,Puigserver P

CLK2 inhibition ameliorates autistic features associated with SHANK3 deficiency.
Science (New York, N.Y.)351(6278)1199-203(2016 Mar)
Bidinosti M,Botta P,Kr?ttner S,Proenca CC,Stoehr N,Bernhard M,Fruh I,Mueller M,Bonenfant D,Voshol H,Carbone W,Neal SJ,McTighe SM,Roma G,Dolmetsch RE,Porter JA,Caroni P,Bouwmeester T,L?thi A,Galimberti I

Cdc2-like kinase 2 suppresses hepatic fatty acid oxidation and ketogenesis through disruption of the PGC-1? and MED1 complex.
Diabetes63(5)1519-32(2014 May)
Tabata M,Rodgers JT,Hall JA,Lee Y,Jedrychowski MP,Gygi SP,Puigserver P

The highly conserved LAMMER/CLK2 protein kinases prevent germ cell overproliferation in Drosophila.
Developmental biology376(2)163-70(2013 Apr)
Zhao S,Chen D,Geng Q,Wang Z