Cd2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cd2
Entrez Gene ID	12481
Full Name	CD2 antigen
Synonyms	LFA-2,Ly-37,Ly37
General protein information	Preferred Names CD2 antigen Names T-cell surface antigen CD2 LFA-3 receptor T-cell surface antigen T11/Leu-5 lymphocyte antigen 37
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 F2.2\|3 44.17 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013486.2	NP_038514.1	T-cell surface antigen CD2 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cd2	NP_036962.1
Mus musculus (house mouse)	Cd2	NP_038514.1
Homo sapiens (human)	CD2	NP_001758.2
Pan troglodytes (chimpanzee)	CD2	NP_001180592.1
	CD2	XP_001112881.1
Bos taurus (cattle)	CD2	NP_001011676.2
Gallus gallus (chicken)	CD2	XP_001232013.2
Canis lupus familiaris (dog)	CD2	XP_849219.1
Xenopus tropicalis (tropical clawed frog)	LOC100487379	XP_002943736.1

Gene Ontology
Bibliography

Related articles in PubMed

Metabolically inactive insulin analogue does not prevent autoimmune diabetes in NOD mice.
Gr?nholm J, Pagni PP, Pham MN, Gibson CB, Macomber PF, Vela JL, von Herrath M, Lenardo MJ
Diabetologia60(8)1475-1482(2017 Aug)

Ptpn22 and Cd2 Variations Are Associated with Altered Protein Expression and Susceptibility to Type 1 Diabetes in Nonobese Diabetic Mice.
Fraser HI, Howlett S, Clark J, Rainbow DB, Stanford SM, Wu DJ, Hsieh YW, Maine CJ, Christensen M, Kuchroo V, Sherman LA, Podolin PL, Todd JA, Steward CA, Peterson LB, Bottini N, Wicker LS
Journal of immunology (Baltimore, Md. : 1950)195(10)4841-52(2015 Nov)

The methylcytosine dioxygenase Tet2 promotes DNA demethylation and activation of cytokine gene expression in T cells.
Ichiyama K, Chen T, Wang X, Yan X, Kim BS, Tanaka S, Ndiaye-Lobry D, Deng Y, Zou Y, Zheng P, Tian Q, Aifantis I, Wei L, Dong C
Immunity42(4)613-26(2015 Apr)

Overexpression of RhoH Permits to Bypass the Pre-TCR Checkpoint.
Tamehiro N, Oda H, Shirai M, Suzuki H
PloS one10(6)e0131047(2015)

The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells.
Liang Y, Cucchetti M, Roncagalli R, Yokosuka T, Malzac A, Bertosio E, Imbert J, Nijman IJ, Suchanek M, Saito T, W?lfing C, Malissen B, Malissen M
Nature immunology14(8)858-66(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ptpn22 and Cd2 Variations Are Associated with Altered Protein Expression and Susceptibility to Type 1 Diabetes
Title: Ptpn22 and Cd2 Variations Are Associated with Altered Protein Expression and Susceptibility to Type 1 Diabetes in Nonobese Diabetic Mice.

CD2 stimuli were mandatory for Treg cell survival with reduced Bim expression, but CD2 may not function as a direct receptor for molecules on stromal cells.
Title: CD2-mediated regulation of peripheral CD4(+) ?CD25(+) regulatory T-cell apoptosis accompanied by down-regulation of Bim.

CD244 inhibition and activation depends on CD2 and phospholipase C-gamma1
Title: Inhibition and activation by CD244 depends on CD2 and phospholipase C-gamma1.

nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse
Title: Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse.

CD2 deficient mice are more resistant to T. gondii infection than wild type mice
Title: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection.

The following Cd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu23323	NM_013486.2 Latest version!	Mus musculus CD2 antigen (Cd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cd2 ( NM_013486.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038514.1 Mus musculus CD2 antigen (Cd2), mRNA.

Price & Availability↑

CloneID	OMu23323
Clone ID Related Accession (Same CDS sequence)	NM_013486.2
Accession Version	NM_013486.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	T-cell surface antigen CD2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC131184.4 and AL645930.15. On Nov 17, 2006 this sequence version replaced NM_013486.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y00023.1, M18934.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849388 [ECO:0000348] ##Evidence-Data-END##

ATGAAATGTA AATTCCTGGG TAGCTTCTTT CTGCTCTTCA GCCTTTCCGG CAAAGGGGCG 
GACTGCAGAG ACAATGAGAC CATCTGGGGT GTCTTGGGTC ATGGCATCAC CCTGAACATC 
CCCAACTTTC AAATGACTGA TGATATTGAT GAGGTGCGAT GGGTAAGGAG GGGCACCCTG 
GTCGCAGAGT TTAAAAGGAA GAAGCCACCT TTTTTGATAT CAGAAACGTA TGAGGTCTTA 
GCAAACGGAT CCCTGAAGAT AAAGAAGCCG ATGATGAGAA ACGACAGTGG CACCTATAAT 
GTAATGGTGT ATGGCACAAA TGGGATGACT AGGCTGGAGA AGGACCTGGA CGTGAGGATT 
CTGGAGAGGG TCTCAAAGCC CATGATCCAC TGGGAATGCC CCAACACAAC CCTGACCTGT 
GCGGTCTTGC AAGGGACAGA TTTTGAACTG AAGCTGTATC AAGGGGAAAC ACTACTCAAT 
AGTCTCCCCC AGAAGAACAT GAGTTACCAG TGGACCAACC TGAACGCACC ATTCAAGTGT 
GAGGCGATAA ACCCGGTCAG CAAGGAGTCT AAGATGGAAG TTGTTAACTG TCCAGAGAAA 
GGTCTGTCCT TCTATGTCAC AGTGGGGGTC GGTGCAGGAG GACTCCTCTT GGTGCTCTTG 
GTGGCGCTTT TTATTTTCTG TATCTGCAAG AGGAGAAAAC GGAACAGGAG GAGAAAAGAT 
GAAGAGCTGG AAATAAAAGC TTCCAGAACA AGCACTGTGG AAAGGGGCCC CAAGCCGCAC 
TCAACCCCAG CCGCAGCAGC GCAGAATTCA GTGGCGCTCC AAGCTCCTCC TCCACCTGGC 
CATCACCTCC AGACACCTGG CCATCGTCCC TTGCCTCCAG GCCACCGTAC CCGTGAGCAC 
CAGCAGAAGA AGAGACCTCC TCCATCAGGC ACACAGATTC ACCAGCAGAA AGGCCCTCCT 
TTACCCAGAC CCCGAGTTCA GCCAAAACCT CCCTGTGGGA GTGGAGATGG TGTTTCACTG 
CCGCCCCCTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038514.1
CDS	61..1095
Translation	MKCKFLGSFFLLFSLSGKGADCRDNETIWGVLGHGITLNIPNFQMTDDIDEVRWVRRGTLVAEFKRKKPPFLISETYEVLANGSLKIKKPMMRNDSGTYNVMVYGTNGMTRLEKDLDVRILERVSKPMIHWECPNTTLTCAVLQGTDFELKLYQGETLLNSLPQKNMSYQWTNLNAPFKCEAINPVSKESKMEVVNCPEKGLSFYVTVGVGAGGLLLVLLVALFIFCICKRRKRNRRRKDEELEIKASRTSTVERGPKPHSTPAAAAQNSVALQAPPPPGHHLQTPGHRPLPPGHRTREHQQKKRPPPSGTQIHQQKGPPLPRPRVQPKPPCGSGDGVSLPPPN

Target ORF information:

RefSeq Version	NM_013486.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CD2 antigen (Cd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013486.2

ORF Insert Sequence:

ATGAAATGTA AATTCCTGGG TAGCTTCTTT CTGCTCTTCA GCCTTTCCGG CAAAGGGGCG 
GACTGCAGAG ACAATGAGAC CATCTGGGGT GTCTTGGGTC ATGGCATCAC CCTGAACATC 
CCCAACTTTC AAATGACTGA TGATATTGAT GAGGTGCGAT GGGTAAGGAG GGGCACCCTG 
GTCGCAGAGT TTAAAAGGAA GAAGCCACCT TTTTTGATAT CAGAAACGTA TGAGGTCTTA 
GCAAACGGAT CCCTGAAGAT AAAGAAGCCG ATGATGAGAA ACGACAGTGG CACCTATAAT 
GTAATGGTGT ATGGCACAAA TGGGATGACT AGGCTGGAGA AGGACCTGGA CGTGAGGATT 
CTGGAGAGGG TCTCAAAGCC CATGATCCAC TGGGAATGCC CCAACACAAC CCTGACCTGT 
GCGGTCTTGC AAGGGACAGA TTTTGAACTG AAGCTGTATC AAGGGGAAAC ACTACTCAAT 
AGTCTCCCCC AGAAGAACAT GAGTTACCAG TGGACCAACC TGAACGCACC ATTCAAGTGT 
GAGGCGATAA ACCCGGTCAG CAAGGAGTCT AAGATGGAAG TTGTTAACTG TCCAGAGAAA 
GGTCTGTCCT TCTATGTCAC AGTGGGGGTC GGTGCAGGAG GACTCCTCTT GGTGCTCTTG 
GTGGCGCTTT TTATTTTCTG TATCTGCAAG AGGAGAAAAC GGAACAGGAG GAGAAAAGAT 
GAAGAGCTGG AAATAAAAGC TTCCAGAACA AGCACTGTGG AAAGGGGCCC CAAGCCGCAC 
TCAACCCCAG CCGCAGCAGC GCAGAATTCA GTGGCGCTCC AAGCTCCTCC TCCACCTGGC 
CATCACCTCC AGACACCTGG CCATCGTCCC TTGCCTCCAG GCCACCGTAC CCGTGAGCAC 
CAGCAGAAGA AGAGACCTCC TCCATCAGGC ACACAGATTC ACCAGCAGAA AGGCCCTCCT 
TTACCCAGAC CCCGAGTTCA GCCAAAACCT CCCTGTGGGA GTGGAGATGG TGTTTCACTG 
CCGCCCCCTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Metabolically inactive insulin analogue does not prevent autoimmune diabetes in NOD mice.
Diabetologia60(8)1475-1482(2017 Aug)
Gr?nholm J,Pagni PP,Pham MN,Gibson CB,Macomber PF,Vela JL,von Herrath M,Lenardo MJ

Ptpn22 and Cd2 Variations Are Associated with Altered Protein Expression and Susceptibility to Type 1 Diabetes in Nonobese Diabetic Mice.
Journal of immunology (Baltimore, Md. : 1950)195(10)4841-52(2015 Nov)
Fraser HI,Howlett S,Clark J,Rainbow DB,Stanford SM,Wu DJ,Hsieh YW,Maine CJ,Christensen M,Kuchroo V,Sherman LA,Podolin PL,Todd JA,Steward CA,Peterson LB,Bottini N,Wicker LS

The methylcytosine dioxygenase Tet2 promotes DNA demethylation and activation of cytokine gene expression in T cells.
Immunity42(4)613-26(2015 Apr)
Ichiyama K,Chen T,Wang X,Yan X,Kim BS,Tanaka S,Ndiaye-Lobry D,Deng Y,Zou Y,Zheng P,Tian Q,Aifantis I,Wei L,Dong C

Overexpression of RhoH Permits to Bypass the Pre-TCR Checkpoint.
PloS one10(6)e0131047(2015)
Tamehiro N,Oda H,Shirai M,Suzuki H

The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells.
Nature immunology14(8)858-66(2013 Aug)
Liang Y,Cucchetti M,Roncagalli R,Yokosuka T,Malzac A,Bertosio E,Imbert J,Nijman IJ,Suchanek M,Saito T,W?lfing C,Malissen B,Malissen M