Cacna1g cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cacna1g
Entrez Gene ID	12291
Full Name	calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms	Cav3.1d,[a]1G,a1G,alpha-1G,mKIAA1123
General protein information	Preferred Names calcium channel, voltage-dependent, T type, alpha 1G subunit Names voltage-dependent T-type calcium channel subunit alpha-1G
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 D

mRNA and Protein(s)

mRNA	Protein	Name
XM_006532069.3	XP_006532132.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
XM_011248690.2	XP_011246992.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
XM_006532076.3	XP_006532139.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X13
XM_011248693.2	XP_011246995.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X15
XM_006532083.3	XP_006532146.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X21
XM_006532085.3	XP_006532148.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X26
XM_006532086.3	XP_006532149.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X28
XM_011248700.2	XP_011247002.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X34
XM_011248687.3	XP_011246989.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
XM_006532072.4	XP_006532135.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
XM_011248692.3	XP_011246994.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X14
XM_006532084.4	XP_006532147.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X25
XM_006532086.4	XP_006532149.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X29
XM_017314234.2	XP_017169723.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X30
XM_030245497.1	XP_030101357.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X33
XM_011248688.2	XP_011246990.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
XM_011248689.2	XP_011246991.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
XM_006532073.3	XP_006532136.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X10
XM_006532074.3	XP_006532137.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X11
XM_011248692.2	XP_011246994.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X14
XM_006532082.3	XP_006532145.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X20
XM_011248695.2	XP_011246997.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X22
XM_011248696.2	XP_011246998.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X23
XM_011248689.3	XP_011246991.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
XM_011248691.3	XP_011246993.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
XM_006532073.4	XP_006532136.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X10
XM_006532083.4	XP_006532146.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X22
XM_011248697.3	XP_011246999.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X26
XM_011248698.3	XP_011247000.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X28
XM_017314235.2	XP_017169724.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X34
XM_011248700.3	XP_011247002.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X36
NM_009783.3	NP_033913.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform a
XM_006532070.3	XP_006532133.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
XM_011248691.2	XP_011246993.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
XM_006532081.3	XP_006532144.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X19
XM_017314235.1	XP_017169724.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X32
XM_011248688.3	XP_011246990.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
XM_006532081.4	XP_006532144.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X20
NM_001177890.1	NP_001171361.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform d
XM_006532072.3	XP_006532135.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
XM_006532075.3	XP_006532138.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X12
XM_006532079.3	XP_006532142.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X16
XM_011248697.2	XP_011246999.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X25
XM_011248698.2	XP_011247000.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X27
XM_017314234.1	XP_017169723.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X29
XM_006532087.3	XP_006532150.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X30
XM_011248686.3	XP_011246988.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
XM_006532069.4	XP_006532132.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
XM_006532075.4	XP_006532138.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X12
XM_011248693.3	XP_011246995.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X15
XM_006532080.4	XP_006532143.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X19
XM_006532087.4	XP_006532150.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X31
XM_006532088.4	XP_006532151.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X32
XM_011248699.3	XP_011247001.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X35
NM_001112813.2	NP_001106284.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform b
XM_011248686.2	XP_011246988.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
XM_011248687.2	XP_011246989.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
XM_011248694.2	XP_011246996.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X17
XM_006532080.3	XP_006532143.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X18
XM_006532084.3	XP_006532147.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X24
XM_006532088.3	XP_006532151.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X31
XM_011248699.2	XP_011247001.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X33
XM_006532070.4	XP_006532133.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
XM_011248690.3	XP_011246992.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
XM_006532074.4	XP_006532137.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X11
XM_006532076.4	XP_006532139.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X13
XM_006532079.4	XP_006532142.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X16
XM_011248694.3	XP_011246996.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X17
XM_030245496.1	XP_030101356.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X18
XM_006532082.4	XP_006532145.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X21
XM_011248695.3	XP_011246997.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X23
XM_011248696.3	XP_011246998.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X24
XM_006532085.4	XP_006532148.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X27
NM_001177888.1	NP_001171359.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform c

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACNA1G	NP_938191.2
Danio rerio (zebrafish)	cacna1g	XP_005156372.1
Bos taurus (cattle)	CACNA1G	NP_001180069.1
Xenopus tropicalis (tropical clawed frog)	cacna1g	XP_004918544.1
Mus musculus (house mouse)	Cacna1g	NP_001106284.1
Rattus norvegicus (Norway rat)	Cacna1g	NP_113789.2
Pan troglodytes (chimpanzee)	CACNA1G	XP_511884.2
Gallus gallus (chicken)	CACNA1G	XP_004946372.1
Canis lupus familiaris (dog)	CACNA1G	XP_005624708.1

Gene Ontology
Bibliography

Related articles in PubMed

Increasing T-Type calcium channel activity by ?-adrenergic stimulation contributes to ?-adrenergic regulation of heart rates.
Li Y, Zhang X, Zhang C, Zhang X, Li Y, Qi Z, Szeto C, Tang M, Peng Y, Molkentin JD, Houser SR, Xie M, Chen X
The Journal of physiology(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data, including data from studies using knockout mice, suggest that acetylcholine- (Ach-)induced vasorelaxation/vasodilatation of intrapulmonary arteries is reduced in pulmonary hypertension, but is still dependent on Cav3.1 activity; thus, Cav3.1 contributes to intrapulmonary vascular reactivity by controlling calcium signaling in arterial endothelium and Ach-induced vasorelaxation/vasodilatation.
Title: T-type voltage gated calcium channels are involved in endothelium-dependent relaxation of mice pulmonary artery.

Cacna1g exclusively expressed in serosal PDGFRalpha+ cells is a new pathological marker for gastrointestinal diseases.
Title: Transcriptome analysis of PDGFR?+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFR?+ cell hyperplasia.

These results provide support for Cacna1g as an epilepsy modifier gene and suggest that modulation of Cav3.1 may be an effective therapeutic strategy.
Title: Cacna1g is a genetic modifier of epilepsy caused by mutation of voltage-gated sodium channel Scn2a.

Localized Cav3.1 knockdown in the medial septum selectively enhanced object exploration, whereas the null mutant (KO) mice showed enhanced-object exploration as well as open-field exploration.
Title: Medial septal GABAergic projection neurons promote object exploration behavior and type 2 theta rhythm.

Mice deficient for CaV3.1 were resistant to the induction of experimental autoimmune encephalomyelitis and had reduced productions of the granulocyte-macrophage colony-stimulating factor by central nervous system-infiltrating Th1 and Th17 cells.
Title: Low-Voltage-Activated CaV3.1 Calcium Channels Shape T Helper Cell Cytokine Profiles.

The following Cacna1g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna1g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu67281	XM_011248699.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu67282	XM_011248700.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu77716	XM_017314235.2 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu45464	XM_006532069.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu45465	XM_006532070.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu45467	XM_006532072.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu45468	XM_006532073.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu45469	XM_006532074.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu45470	XM_006532075.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu45471	XM_006532076.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu45474	XM_006532079.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu45475	XM_006532080.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu45476	XM_006532081.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu45477	XM_006532082.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu45478	XM_006532083.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu45479	XM_006532084.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu45480	XM_006532085.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu45481	XM_006532086.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu45482	XM_006532087.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu45483	XM_006532088.4 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67268	XM_011248686.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu67269	XM_011248687.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu67270	XM_011248688.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu67271	XM_011248689.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu67272	XM_011248690.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu67273	XM_011248691.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu67274	XM_011248692.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu67275	XM_011248693.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu67276	XM_011248694.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67277	XM_011248695.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67278	XM_011248696.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67279	XM_011248697.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67280	XM_011248698.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu77715	XM_017314234.2 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85603	XM_030245496.1 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu85604	XM_030245497.1 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu16041	NM_001177890.1 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu16069	NM_001112813.2 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1672.30 $2389.00
OMu16165	NM_009783.3 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu16231	NM_001177888.1 Latest version!	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1665.30 $2379.00
View Old Accession Versions >>
OMu45481	XM_006532086.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67274	XM_011248692.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67271	XM_011248689.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu45468	XM_006532073.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu45478	XM_006532083.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67282	XM_011248700.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu67273	XM_011248691.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu77716	XM_017314235.1	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu67270	XM_011248688.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu45476	XM_006532081.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu45467	XM_006532072.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67279	XM_011248697.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67280	XM_011248698.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu77715	XM_017314234.1	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu45464	XM_006532069.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu45470	XM_006532075.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67275	XM_011248693.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu45482	XM_006532087.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67268	XM_011248686.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67269	XM_011248687.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu45475	XM_006532080.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu45479	XM_006532084.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu45483	XM_006532088.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67281	XM_011248699.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu45465	XM_006532070.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67272	XM_011248690.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu45469	XM_006532074.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu45471	XM_006532076.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu45474	XM_006532079.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67276	XM_011248694.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu45477	XM_006532082.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67277	XM_011248695.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67278	XM_011248696.2	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu45480	XM_006532085.3	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cacna1g ( XM_011248699.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247001.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X40, mRNA.

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CloneID	OMu67281
Clone ID Related Accession (Same CDS sequence)	XM_011248699.3 , XM_011248699.2
Accession Version	XM_011248699.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248699.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GGGACAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247001.1
CDS	784..5535
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAGTG

Target ORF information:

RefSeq Version	XM_011248699.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248699.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GGGACAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248700.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247002.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X41, mRNA.

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CloneID	OMu67282
Clone ID Related Accession (Same CDS sequence)	XM_011248700.2 , XM_011248700.3
Accession Version	XM_011248700.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248700.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GGACAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247002.1
CDS	784..5514
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRRTG

Target ORF information:

RefSeq Version	XM_011248700.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248700.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GGACAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_017314235.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169724.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X39, mRNA.

Price & Availability↑

CloneID	OMu77716
Clone ID Related Accession (Same CDS sequence)	XM_017314235.2 , XM_017314235.1
Accession Version	XM_017314235.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314235.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA AGCTGCTCGT CTACGGTCCC TTCGGCTACA TTAAGAATCC CTACAACATC 
TTTGATGGCG TCATTGTGGT CATCAGCGTG TGGGAGATTG TGGGCCAGCA GGGAGGTGGC 
CTGTCGGTGC TGCGGACCTT CCGCCTGATG CGGGTGCTGA AGCTAGTTCG CTTCCTGCCG 
GCACTGCAGC GGCAGCTCGT GGTGCTCATG AAGACCATGG ACAACGTGGC CACCTTCTGC 
ATGCTGCTCA TGCTCTTCAT CTTCATCTTC AGCATCCTGG GCATGCACCT TTTTGGTTGC 
AAGTTCGCAT CTGAACGGGA TGGGGACACG TTGCCAGACC GGAAGAATTT TGACTCCCTG 
CTCTGGGCCA TTGTCACTGT CTTTCAGATT CTGACTCAGG AAGACTGGAA TAAAGTCCTT 
TACAACGGCA TGGCCTCCAC GTCATCTTGG GCTGCTCTTT ACTTCATCGC CCTCATGACT 
TTTGGCAACT ACGTGCTCTT TAACCTGCTT GTCGCCATTC TCGTGGAGGG TTTCCAGGCA 
GAGGAAATCG GCAAACGGGA AGATACGAGT GGACAGTTAA GCTGTATTCA GCTGCCTGTC 
AACTCCCAGG GGGGAGATGC CACCAAGTCT GAGTCAGAGC CTGATTTCTT TTCGCCCAGT 
GTGGATGGTG ATGGGGACAG GAAGAAGCGC TTGGCCCTGG TGGCCTTGGG AGAACACTCG 
GAACTACGAA AGAGCCTTTT GCCACCTCTC ATCATCCACA CAGCTGCTAC ACCGATGTCA 
CTGCCCAAGA GCTCCAGCAC AGGTGTGGGG GAAGCACTGG GCTCTGGCTC TCGCCGCACC 
AGTAGCAGTG GGTCCGCTGA GCCTGGAACT GCCCATCATG AGATGAAATC ACCGCCAAGT 
GCCCGAAGCT CCCCGCACAG TCCCTGGAGC GCAGCAAGCA GCTGGACCAG CAGGCGCTCC 
AGCCGGAACA GCCTGGGCCG GGCCCCCAGC CTGAAGCGTA GGAGCCCAAG CGGGGAGCGG 
AGGTCCCTGC TGTCTGGAGA GGGTCAGGAG AGCCAGGATG AGGAGGAGAG TTCAGAAGAG 
GACCGGGCCA GCCCAGCAGG CAGTGATCAT CGCCACAGGG GTTCCTTGGA ACGTGAGGCC 
AAGAGTTCCT TTGACCTGCC TGACACCCTG CAGGTGCCCG GGCTTCATCG AACAGCCAGC 
GGTCGGAGCT CTGCCTCTGA ACACCAAGAC TGTAATGGCA AGTCGGCTTC AGGGCGTTTG 
GCCCGCACCC TGCGGGCTGA TGACCCCCCA CTGGATGGGG ATGATGGCGA TGATGAGGGC 
AACCTGAGCA AAGGGGAACG CTTACGAGCC TGGGTCCGAG CCCGGCTCCC TGCCTGTTGC 
CGAGAGCGAG ATTCCTGGTC TGCCTATATC TTCCCTCCCC AGTCAAGGTT TCGTCTCCTG 
TGTCACCGGA TCATCACCCA CAAGATGTTT GACCATGTGG TCCTCGTCAT CATCTTCCTC 
AACTGTATCA CCATCGCTAT GGAGCGCCCC AAAATTGACC CCCACAGCGC TGAACGCATC 
TTCCTGACCC TCTCCAACTA CATCTTCACG GCAGTCTTCC TGGCTGAAAT GACAGTGAAG 
GTGGTGGCAC TGGGCTGGTG CTTTGGGGAG CAGGCCTACC TGCGCAGCAG CTGGAACGTG 
CTGGACGGCT TGCTGGTGCT CATCTCTGTC ATCGACATCC TGGTGTCCAT GGTCTCTGAC 
AGCGGCACCA AGATTCTCGG CATGCTGAGG GTGCTGCGGC TGCTGCGGAC CCTACGTCCA 
CTCAGGGTCA TCAGCCGGGC CCAGGGGCTG AAGCTGGTGG TAGAGACTCT GATGTCATCC 
CTCAAACCCA TTGGCAACAT TGTGGTCATC TGCTGTGCCT TCTTCATCAT TTTTGGAATT 
CTTGGGGTGC AGCTCTTCAA AGGGAAGTTC TTCGTGTGTC AGGGTGAGGA CACCAGGAAC 
ATCACTAACA AGTCCGACTG TGCTGAGGCC AGTTACCGGT GGGTCCGGCA CAAGTACAAC 
TTTGACAACC TGGGCCAGGC TCTGATGTCC CTGTTTGTGC TGGCCTCCAA GGATGGCTGG 
GTTGACATCA TGTATGATGG ACTGGATGCT GTGGGAGTGG ACCAGCAGCC CATCATGAAC 
CACAACCCTT GGATGCTGCT CTACTTCATC TCCTTCCTCC TCATCGTGGC CTTCTTCGTC 
CTGAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCAGGCA GCACCAGGAG 
GAGGAGGAGG CGCGGCGGCG GGAGGAGAAG CGACTAAAGA GGCTGGAGAA AAAGAGAAGG 
AGTAAGGAGA AGCAGATGGC CGATCTAATG TTGGACGATG TAATTGCTTC CGGCAGCTCA 
GCCAGCGCTG CGTCAGAAGC CCAGTGCAAA CCCTACTACT CTGACTACTC GCGCTTCCGG 
CTCCTCGTCC ACCACCTGTG TACCAGCCAC TACCTGGACC TCTTCATCAC TGGTGTCATC 
GGGCTGAATG TGGTCACGAT GGCCATGGAA CATTACCAGC AGCCCCAGAT CCTGGACGAG 
GCTCTGAAGA TCTGCAACTA CATCTTTACC GTCATCTTTG TCTTGGAGTC AGTATTCAAA 
CTTGTGGCCT TCGGCTTCCG CCGGTTCTTC CAGGACAGGT GGAACCAGCT GGACCTGGCT 
ATTGTGCTTC TGTCCATCAT GGGCATCACG CTGGAAGAGA TTGAGGTCAA TGCTTCACTG 
CCCATCAACC CCACCATCAT CCGTATCATG AGGGTGCTCC GCATTGCTCG AGTTCTGAAG 
CTGTTGAAGA TGGCTGTGGG CATGCGGGCA CTGCTGGACA CGGTGATGCA GGCCCTGCCC 
CAGGTGGGGA ACCTGGGACT TCTCTTCATG CTATTATTTT TCATCTTTGC AGCTCTGGGC 
GTGGAGCTCT TTGGAGACCT GGAGTGTGAT GAGACACACC CTTGTGAGGG CTTGGGCCGG 
CATGCCACCT TTAGGAACTT TGGTATGGCC TTTCTGACCC TCTTCCGAGT CTCCACTGGT 
GACAACTGGA ATGGTATTAT GAAGGACACC CTCCGGGACT GTGACCAGGA GTCCACCTGC 
TACAACACCG TCATCTCACC CATCTACTTC GTGTCCTTCG TGCTGACGGC CCAGTTTGTG 
CTGGTCAACG TGGTCATAGC CGTGCTGATG AAGCACCTGG AAGAGAGCAA CAAAGAGGCC 
AAGGAGGAGG CGGAGTTGGA GGCGGAGCTG GAGCTAGAGA TGAAGACACT CAGCCCGCAG 
CCCCACTCCC CGCTGGGCAG CCCCTTCCTC TGGCCTGGGG TGGAAGGTGT CAATAGCCCT 
GACAGCCCTA AGCCTGGGGC TCCACACACC ACGGCCCACA TTGGAGCAGC CTCTTCAGGC 
TTCTCCCTTG AGCACCCCAC GGACAGACAG CTGTTTGACA CCATCTCCCT GCTGATCCAG 
GGCTCCCTGG AGGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTTCCGCCCC CAGCCCGGGC GACTCCGACC CACAGATCCC TCTAGCTGAG 
ATGGAGGCTC TGTCTCTGAC GTCAGAGATT GTGTCTGAAC CGTCCTGCTC TCTAGCTCTG 
ACGGATGACT CTTTGCCTGA TGACACTCAC ACACTCTTAC TTAGTGCCCT GGAGAGCAAT 
ATGGTACCTC ACACTGAGGA GGGGCCAGTC CCCCTAGGAC CAGACCTGCT GACTGTGAGG 
AAGTCTGGTG TCAGCCGGAC ACACTCTCTG CCCAATGACA GCTACATGTG CCGCAATGGG 
AGCACTGCCG AGAGATCCCT AGGACACAGG GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC 
TCCATCTTGT CTGTTCACTC CCAACCAGCA GACACCAGCT GCATCCTACA GCTTCCCAAA 
GATGCACACT ATCTGCTCCA GCCTCATGGG GCTCCCACCT GGGGCGCCAT CCCTAAACTA 
CCCCCACCTG GCCGCTCCCC TCTGGCTCAG AGGCCTCTCA GGCGCCAGGC AGCAATAAGG 
ACTGACTCCC TGGACGTGCA GGGCCTGGGT AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT 
GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA TCCTTCTGGG GCGGGTCGAG CATCCAGGTG 
CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC TCCAAGCACA TCCGCCTGCC AGCCCCTTGC 
CCAGGCCTGG AACCCAGCTG GGCCAAGGAC CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA GCAGTCAGGA AGAACCCCTG 
TCCCCACGGG ACTTGAAAAA ATGCTACAGT GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT 
GGGTCCTGGC TAGACGAACA GAGGAGACAC TCCATCGCTG TCAGCTGCCT GGACAGCGGC 
TCCCAGCCCC GCCTATGTCC AAGCCCCTCA AGCCTCGGGG GCCAACCTCT TGGGGGCCCT 
GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA CCCAGTATCT CTATAGACCC CCCGGAGAGC 
CAGGGCCCTC GGCCCCCATG CAGTCCTGGC GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT 
GACTCGAAGG ATCCCTCGGC CTCCAGCCCC CTTGACAGCA CGGCTGCCTC ACCCTCCCCA 
AAGAAAGATG CGCTGAGTCT CTCTGGTTTG TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169724.1
CDS	188..4987
Translation	MLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_017314235.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314235.2

ORF Insert Sequence:

ATGCTGCTGA AGCTGCTCGT CTACGGTCCC TTCGGCTACA TTAAGAATCC CTACAACATC 
TTTGATGGCG TCATTGTGGT CATCAGCGTG TGGGAGATTG TGGGCCAGCA GGGAGGTGGC 
CTGTCGGTGC TGCGGACCTT CCGCCTGATG CGGGTGCTGA AGCTAGTTCG CTTCCTGCCG 
GCACTGCAGC GGCAGCTCGT GGTGCTCATG AAGACCATGG ACAACGTGGC CACCTTCTGC 
ATGCTGCTCA TGCTCTTCAT CTTCATCTTC AGCATCCTGG GCATGCACCT TTTTGGTTGC 
AAGTTCGCAT CTGAACGGGA TGGGGACACG TTGCCAGACC GGAAGAATTT TGACTCCCTG 
CTCTGGGCCA TTGTCACTGT CTTTCAGATT CTGACTCAGG AAGACTGGAA TAAAGTCCTT 
TACAACGGCA TGGCCTCCAC GTCATCTTGG GCTGCTCTTT ACTTCATCGC CCTCATGACT 
TTTGGCAACT ACGTGCTCTT TAACCTGCTT GTCGCCATTC TCGTGGAGGG TTTCCAGGCA 
GAGGAAATCG GCAAACGGGA AGATACGAGT GGACAGTTAA GCTGTATTCA GCTGCCTGTC 
AACTCCCAGG GGGGAGATGC CACCAAGTCT GAGTCAGAGC CTGATTTCTT TTCGCCCAGT 
GTGGATGGTG ATGGGGACAG GAAGAAGCGC TTGGCCCTGG TGGCCTTGGG AGAACACTCG 
GAACTACGAA AGAGCCTTTT GCCACCTCTC ATCATCCACA CAGCTGCTAC ACCGATGTCA 
CTGCCCAAGA GCTCCAGCAC AGGTGTGGGG GAAGCACTGG GCTCTGGCTC TCGCCGCACC 
AGTAGCAGTG GGTCCGCTGA GCCTGGAACT GCCCATCATG AGATGAAATC ACCGCCAAGT 
GCCCGAAGCT CCCCGCACAG TCCCTGGAGC GCAGCAAGCA GCTGGACCAG CAGGCGCTCC 
AGCCGGAACA GCCTGGGCCG GGCCCCCAGC CTGAAGCGTA GGAGCCCAAG CGGGGAGCGG 
AGGTCCCTGC TGTCTGGAGA GGGTCAGGAG AGCCAGGATG AGGAGGAGAG TTCAGAAGAG 
GACCGGGCCA GCCCAGCAGG CAGTGATCAT CGCCACAGGG GTTCCTTGGA ACGTGAGGCC 
AAGAGTTCCT TTGACCTGCC TGACACCCTG CAGGTGCCCG GGCTTCATCG AACAGCCAGC 
GGTCGGAGCT CTGCCTCTGA ACACCAAGAC TGTAATGGCA AGTCGGCTTC AGGGCGTTTG 
GCCCGCACCC TGCGGGCTGA TGACCCCCCA CTGGATGGGG ATGATGGCGA TGATGAGGGC 
AACCTGAGCA AAGGGGAACG CTTACGAGCC TGGGTCCGAG CCCGGCTCCC TGCCTGTTGC 
CGAGAGCGAG ATTCCTGGTC TGCCTATATC TTCCCTCCCC AGTCAAGGTT TCGTCTCCTG 
TGTCACCGGA TCATCACCCA CAAGATGTTT GACCATGTGG TCCTCGTCAT CATCTTCCTC 
AACTGTATCA CCATCGCTAT GGAGCGCCCC AAAATTGACC CCCACAGCGC TGAACGCATC 
TTCCTGACCC TCTCCAACTA CATCTTCACG GCAGTCTTCC TGGCTGAAAT GACAGTGAAG 
GTGGTGGCAC TGGGCTGGTG CTTTGGGGAG CAGGCCTACC TGCGCAGCAG CTGGAACGTG 
CTGGACGGCT TGCTGGTGCT CATCTCTGTC ATCGACATCC TGGTGTCCAT GGTCTCTGAC 
AGCGGCACCA AGATTCTCGG CATGCTGAGG GTGCTGCGGC TGCTGCGGAC CCTACGTCCA 
CTCAGGGTCA TCAGCCGGGC CCAGGGGCTG AAGCTGGTGG TAGAGACTCT GATGTCATCC 
CTCAAACCCA TTGGCAACAT TGTGGTCATC TGCTGTGCCT TCTTCATCAT TTTTGGAATT 
CTTGGGGTGC AGCTCTTCAA AGGGAAGTTC TTCGTGTGTC AGGGTGAGGA CACCAGGAAC 
ATCACTAACA AGTCCGACTG TGCTGAGGCC AGTTACCGGT GGGTCCGGCA CAAGTACAAC 
TTTGACAACC TGGGCCAGGC TCTGATGTCC CTGTTTGTGC TGGCCTCCAA GGATGGCTGG 
GTTGACATCA TGTATGATGG ACTGGATGCT GTGGGAGTGG ACCAGCAGCC CATCATGAAC 
CACAACCCTT GGATGCTGCT CTACTTCATC TCCTTCCTCC TCATCGTGGC CTTCTTCGTC 
CTGAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCAGGCA GCACCAGGAG 
GAGGAGGAGG CGCGGCGGCG GGAGGAGAAG CGACTAAAGA GGCTGGAGAA AAAGAGAAGG 
AGTAAGGAGA AGCAGATGGC CGATCTAATG TTGGACGATG TAATTGCTTC CGGCAGCTCA 
GCCAGCGCTG CGTCAGAAGC CCAGTGCAAA CCCTACTACT CTGACTACTC GCGCTTCCGG 
CTCCTCGTCC ACCACCTGTG TACCAGCCAC TACCTGGACC TCTTCATCAC TGGTGTCATC 
GGGCTGAATG TGGTCACGAT GGCCATGGAA CATTACCAGC AGCCCCAGAT CCTGGACGAG 
GCTCTGAAGA TCTGCAACTA CATCTTTACC GTCATCTTTG TCTTGGAGTC AGTATTCAAA 
CTTGTGGCCT TCGGCTTCCG CCGGTTCTTC CAGGACAGGT GGAACCAGCT GGACCTGGCT 
ATTGTGCTTC TGTCCATCAT GGGCATCACG CTGGAAGAGA TTGAGGTCAA TGCTTCACTG 
CCCATCAACC CCACCATCAT CCGTATCATG AGGGTGCTCC GCATTGCTCG AGTTCTGAAG 
CTGTTGAAGA TGGCTGTGGG CATGCGGGCA CTGCTGGACA CGGTGATGCA GGCCCTGCCC 
CAGGTGGGGA ACCTGGGACT TCTCTTCATG CTATTATTTT TCATCTTTGC AGCTCTGGGC 
GTGGAGCTCT TTGGAGACCT GGAGTGTGAT GAGACACACC CTTGTGAGGG CTTGGGCCGG 
CATGCCACCT TTAGGAACTT TGGTATGGCC TTTCTGACCC TCTTCCGAGT CTCCACTGGT 
GACAACTGGA ATGGTATTAT GAAGGACACC CTCCGGGACT GTGACCAGGA GTCCACCTGC 
TACAACACCG TCATCTCACC CATCTACTTC GTGTCCTTCG TGCTGACGGC CCAGTTTGTG 
CTGGTCAACG TGGTCATAGC CGTGCTGATG AAGCACCTGG AAGAGAGCAA CAAAGAGGCC 
AAGGAGGAGG CGGAGTTGGA GGCGGAGCTG GAGCTAGAGA TGAAGACACT CAGCCCGCAG 
CCCCACTCCC CGCTGGGCAG CCCCTTCCTC TGGCCTGGGG TGGAAGGTGT CAATAGCCCT 
GACAGCCCTA AGCCTGGGGC TCCACACACC ACGGCCCACA TTGGAGCAGC CTCTTCAGGC 
TTCTCCCTTG AGCACCCCAC GGACAGACAG CTGTTTGACA CCATCTCCCT GCTGATCCAG 
GGCTCCCTGG AGGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTTCCGCCCC CAGCCCGGGC GACTCCGACC CACAGATCCC TCTAGCTGAG 
ATGGAGGCTC TGTCTCTGAC GTCAGAGATT GTGTCTGAAC CGTCCTGCTC TCTAGCTCTG 
ACGGATGACT CTTTGCCTGA TGACACTCAC ACACTCTTAC TTAGTGCCCT GGAGAGCAAT 
ATGGTACCTC ACACTGAGGA GGGGCCAGTC CCCCTAGGAC CAGACCTGCT GACTGTGAGG 
AAGTCTGGTG TCAGCCGGAC ACACTCTCTG CCCAATGACA GCTACATGTG CCGCAATGGG 
AGCACTGCCG AGAGATCCCT AGGACACAGG GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC 
TCCATCTTGT CTGTTCACTC CCAACCAGCA GACACCAGCT GCATCCTACA GCTTCCCAAA 
GATGCACACT ATCTGCTCCA GCCTCATGGG GCTCCCACCT GGGGCGCCAT CCCTAAACTA 
CCCCCACCTG GCCGCTCCCC TCTGGCTCAG AGGCCTCTCA GGCGCCAGGC AGCAATAAGG 
ACTGACTCCC TGGACGTGCA GGGCCTGGGT AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT 
GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA TCCTTCTGGG GCGGGTCGAG CATCCAGGTG 
CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC TCCAAGCACA TCCGCCTGCC AGCCCCTTGC 
CCAGGCCTGG AACCCAGCTG GGCCAAGGAC CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA GCAGTCAGGA AGAACCCCTG 
TCCCCACGGG ACTTGAAAAA ATGCTACAGT GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT 
GGGTCCTGGC TAGACGAACA GAGGAGACAC TCCATCGCTG TCAGCTGCCT GGACAGCGGC 
TCCCAGCCCC GCCTATGTCC AAGCCCCTCA AGCCTCGGGG GCCAACCTCT TGGGGGCCCT 
GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA CCCAGTATCT CTATAGACCC CCCGGAGAGC 
CAGGGCCCTC GGCCCCCATG CAGTCCTGGC GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT 
GACTCGAAGG ATCCCTCGGC CTCCAGCCCC CTTGACAGCA CGGCTGCCTC ACCCTCCCCA 
AAGAAAGATG CGCTGAGTCT CTCTGGTTTG TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_017314235.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169724.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X35, mRNA.

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CloneID	OMu77716
Clone ID Related Accession (Same CDS sequence)	XM_017314235.2 , XM_017314235.1
Accession Version	XM_017314235.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA AGCTGCTCGT CTACGGTCCC TTCGGCTACA TTAAGAATCC CTACAACATC 
TTTGATGGCG TCATTGTGGT CATCAGCGTG TGGGAGATTG TGGGCCAGCA GGGAGGTGGC 
CTGTCGGTGC TGCGGACCTT CCGCCTGATG CGGGTGCTGA AGCTAGTTCG CTTCCTGCCG 
GCACTGCAGC GGCAGCTCGT GGTGCTCATG AAGACCATGG ACAACGTGGC CACCTTCTGC 
ATGCTGCTCA TGCTCTTCAT CTTCATCTTC AGCATCCTGG GCATGCACCT TTTTGGTTGC 
AAGTTCGCAT CTGAACGGGA TGGGGACACG TTGCCAGACC GGAAGAATTT TGACTCCCTG 
CTCTGGGCCA TTGTCACTGT CTTTCAGATT CTGACTCAGG AAGACTGGAA TAAAGTCCTT 
TACAACGGCA TGGCCTCCAC GTCATCTTGG GCTGCTCTTT ACTTCATCGC CCTCATGACT 
TTTGGCAACT ACGTGCTCTT TAACCTGCTT GTCGCCATTC TCGTGGAGGG TTTCCAGGCA 
GAGGAAATCG GCAAACGGGA AGATACGAGT GGACAGTTAA GCTGTATTCA GCTGCCTGTC 
AACTCCCAGG GGGGAGATGC CACCAAGTCT GAGTCAGAGC CTGATTTCTT TTCGCCCAGT 
GTGGATGGTG ATGGGGACAG GAAGAAGCGC TTGGCCCTGG TGGCCTTGGG AGAACACTCG 
GAACTACGAA AGAGCCTTTT GCCACCTCTC ATCATCCACA CAGCTGCTAC ACCGATGTCA 
CTGCCCAAGA GCTCCAGCAC AGGTGTGGGG GAAGCACTGG GCTCTGGCTC TCGCCGCACC 
AGTAGCAGTG GGTCCGCTGA GCCTGGAACT GCCCATCATG AGATGAAATC ACCGCCAAGT 
GCCCGAAGCT CCCCGCACAG TCCCTGGAGC GCAGCAAGCA GCTGGACCAG CAGGCGCTCC 
AGCCGGAACA GCCTGGGCCG GGCCCCCAGC CTGAAGCGTA GGAGCCCAAG CGGGGAGCGG 
AGGTCCCTGC TGTCTGGAGA GGGTCAGGAG AGCCAGGATG AGGAGGAGAG TTCAGAAGAG 
GACCGGGCCA GCCCAGCAGG CAGTGATCAT CGCCACAGGG GTTCCTTGGA ACGTGAGGCC 
AAGAGTTCCT TTGACCTGCC TGACACCCTG CAGGTGCCCG GGCTTCATCG AACAGCCAGC 
GGTCGGAGCT CTGCCTCTGA ACACCAAGAC TGTAATGGCA AGTCGGCTTC AGGGCGTTTG 
GCCCGCACCC TGCGGGCTGA TGACCCCCCA CTGGATGGGG ATGATGGCGA TGATGAGGGC 
AACCTGAGCA AAGGGGAACG CTTACGAGCC TGGGTCCGAG CCCGGCTCCC TGCCTGTTGC 
CGAGAGCGAG ATTCCTGGTC TGCCTATATC TTCCCTCCCC AGTCAAGGTT TCGTCTCCTG 
TGTCACCGGA TCATCACCCA CAAGATGTTT GACCATGTGG TCCTCGTCAT CATCTTCCTC 
AACTGTATCA CCATCGCTAT GGAGCGCCCC AAAATTGACC CCCACAGCGC TGAACGCATC 
TTCCTGACCC TCTCCAACTA CATCTTCACG GCAGTCTTCC TGGCTGAAAT GACAGTGAAG 
GTGGTGGCAC TGGGCTGGTG CTTTGGGGAG CAGGCCTACC TGCGCAGCAG CTGGAACGTG 
CTGGACGGCT TGCTGGTGCT CATCTCTGTC ATCGACATCC TGGTGTCCAT GGTCTCTGAC 
AGCGGCACCA AGATTCTCGG CATGCTGAGG GTGCTGCGGC TGCTGCGGAC CCTACGTCCA 
CTCAGGGTCA TCAGCCGGGC CCAGGGGCTG AAGCTGGTGG TAGAGACTCT GATGTCATCC 
CTCAAACCCA TTGGCAACAT TGTGGTCATC TGCTGTGCCT TCTTCATCAT TTTTGGAATT 
CTTGGGGTGC AGCTCTTCAA AGGGAAGTTC TTCGTGTGTC AGGGTGAGGA CACCAGGAAC 
ATCACTAACA AGTCCGACTG TGCTGAGGCC AGTTACCGGT GGGTCCGGCA CAAGTACAAC 
TTTGACAACC TGGGCCAGGC TCTGATGTCC CTGTTTGTGC TGGCCTCCAA GGATGGCTGG 
GTTGACATCA TGTATGATGG ACTGGATGCT GTGGGAGTGG ACCAGCAGCC CATCATGAAC 
CACAACCCTT GGATGCTGCT CTACTTCATC TCCTTCCTCC TCATCGTGGC CTTCTTCGTC 
CTGAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCAGGCA GCACCAGGAG 
GAGGAGGAGG CGCGGCGGCG GGAGGAGAAG CGACTAAAGA GGCTGGAGAA AAAGAGAAGG 
AGTAAGGAGA AGCAGATGGC CGATCTAATG TTGGACGATG TAATTGCTTC CGGCAGCTCA 
GCCAGCGCTG CGTCAGAAGC CCAGTGCAAA CCCTACTACT CTGACTACTC GCGCTTCCGG 
CTCCTCGTCC ACCACCTGTG TACCAGCCAC TACCTGGACC TCTTCATCAC TGGTGTCATC 
GGGCTGAATG TGGTCACGAT GGCCATGGAA CATTACCAGC AGCCCCAGAT CCTGGACGAG 
GCTCTGAAGA TCTGCAACTA CATCTTTACC GTCATCTTTG TCTTGGAGTC AGTATTCAAA 
CTTGTGGCCT TCGGCTTCCG CCGGTTCTTC CAGGACAGGT GGAACCAGCT GGACCTGGCT 
ATTGTGCTTC TGTCCATCAT GGGCATCACG CTGGAAGAGA TTGAGGTCAA TGCTTCACTG 
CCCATCAACC CCACCATCAT CCGTATCATG AGGGTGCTCC GCATTGCTCG AGTTCTGAAG 
CTGTTGAAGA TGGCTGTGGG CATGCGGGCA CTGCTGGACA CGGTGATGCA GGCCCTGCCC 
CAGGTGGGGA ACCTGGGACT TCTCTTCATG CTATTATTTT TCATCTTTGC AGCTCTGGGC 
GTGGAGCTCT TTGGAGACCT GGAGTGTGAT GAGACACACC CTTGTGAGGG CTTGGGCCGG 
CATGCCACCT TTAGGAACTT TGGTATGGCC TTTCTGACCC TCTTCCGAGT CTCCACTGGT 
GACAACTGGA ATGGTATTAT GAAGGACACC CTCCGGGACT GTGACCAGGA GTCCACCTGC 
TACAACACCG TCATCTCACC CATCTACTTC GTGTCCTTCG TGCTGACGGC CCAGTTTGTG 
CTGGTCAACG TGGTCATAGC CGTGCTGATG AAGCACCTGG AAGAGAGCAA CAAAGAGGCC 
AAGGAGGAGG CGGAGTTGGA GGCGGAGCTG GAGCTAGAGA TGAAGACACT CAGCCCGCAG 
CCCCACTCCC CGCTGGGCAG CCCCTTCCTC TGGCCTGGGG TGGAAGGTGT CAATAGCCCT 
GACAGCCCTA AGCCTGGGGC TCCACACACC ACGGCCCACA TTGGAGCAGC CTCTTCAGGC 
TTCTCCCTTG AGCACCCCAC GGACAGACAG CTGTTTGACA CCATCTCCCT GCTGATCCAG 
GGCTCCCTGG AGGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTTCCGCCCC CAGCCCGGGC GACTCCGACC CACAGATCCC TCTAGCTGAG 
ATGGAGGCTC TGTCTCTGAC GTCAGAGATT GTGTCTGAAC CGTCCTGCTC TCTAGCTCTG 
ACGGATGACT CTTTGCCTGA TGACACTCAC ACACTCTTAC TTAGTGCCCT GGAGAGCAAT 
ATGGTACCTC ACACTGAGGA GGGGCCAGTC CCCCTAGGAC CAGACCTGCT GACTGTGAGG 
AAGTCTGGTG TCAGCCGGAC ACACTCTCTG CCCAATGACA GCTACATGTG CCGCAATGGG 
AGCACTGCCG AGAGATCCCT AGGACACAGG GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC 
TCCATCTTGT CTGTTCACTC CCAACCAGCA GACACCAGCT GCATCCTACA GCTTCCCAAA 
GATGCACACT ATCTGCTCCA GCCTCATGGG GCTCCCACCT GGGGCGCCAT CCCTAAACTA 
CCCCCACCTG GCCGCTCCCC TCTGGCTCAG AGGCCTCTCA GGCGCCAGGC AGCAATAAGG 
ACTGACTCCC TGGACGTGCA GGGCCTGGGT AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT 
GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA TCCTTCTGGG GCGGGTCGAG CATCCAGGTG 
CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC TCCAAGCACA TCCGCCTGCC AGCCCCTTGC 
CCAGGCCTGG AACCCAGCTG GGCCAAGGAC CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA GCAGTCAGGA AGAACCCCTG 
TCCCCACGGG ACTTGAAAAA ATGCTACAGT GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT 
GGGTCCTGGC TAGACGAACA GAGGAGACAC TCCATCGCTG TCAGCTGCCT GGACAGCGGC 
TCCCAGCCCC GCCTATGTCC AAGCCCCTCA AGCCTCGGGG GCCAACCTCT TGGGGGCCCT 
GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA CCCAGTATCT CTATAGACCC CCCGGAGAGC 
CAGGGCCCTC GGCCCCCATG CAGTCCTGGC GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT 
GACTCGAAGG ATCCCTCGGC CTCCAGCCCC CTTGACAGCA CGGCTGCCTC ACCCTCCCCA 
AAGAAAGATG CGCTGAGTCT CTCTGGTTTG TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169724.1
CDS	192..4991
Translation	MLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_017314235.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314235.1

ORF Insert Sequence:

ATGCTGCTGA AGCTGCTCGT CTACGGTCCC TTCGGCTACA TTAAGAATCC CTACAACATC 
TTTGATGGCG TCATTGTGGT CATCAGCGTG TGGGAGATTG TGGGCCAGCA GGGAGGTGGC 
CTGTCGGTGC TGCGGACCTT CCGCCTGATG CGGGTGCTGA AGCTAGTTCG CTTCCTGCCG 
GCACTGCAGC GGCAGCTCGT GGTGCTCATG AAGACCATGG ACAACGTGGC CACCTTCTGC 
ATGCTGCTCA TGCTCTTCAT CTTCATCTTC AGCATCCTGG GCATGCACCT TTTTGGTTGC 
AAGTTCGCAT CTGAACGGGA TGGGGACACG TTGCCAGACC GGAAGAATTT TGACTCCCTG 
CTCTGGGCCA TTGTCACTGT CTTTCAGATT CTGACTCAGG AAGACTGGAA TAAAGTCCTT 
TACAACGGCA TGGCCTCCAC GTCATCTTGG GCTGCTCTTT ACTTCATCGC CCTCATGACT 
TTTGGCAACT ACGTGCTCTT TAACCTGCTT GTCGCCATTC TCGTGGAGGG TTTCCAGGCA 
GAGGAAATCG GCAAACGGGA AGATACGAGT GGACAGTTAA GCTGTATTCA GCTGCCTGTC 
AACTCCCAGG GGGGAGATGC CACCAAGTCT GAGTCAGAGC CTGATTTCTT TTCGCCCAGT 
GTGGATGGTG ATGGGGACAG GAAGAAGCGC TTGGCCCTGG TGGCCTTGGG AGAACACTCG 
GAACTACGAA AGAGCCTTTT GCCACCTCTC ATCATCCACA CAGCTGCTAC ACCGATGTCA 
CTGCCCAAGA GCTCCAGCAC AGGTGTGGGG GAAGCACTGG GCTCTGGCTC TCGCCGCACC 
AGTAGCAGTG GGTCCGCTGA GCCTGGAACT GCCCATCATG AGATGAAATC ACCGCCAAGT 
GCCCGAAGCT CCCCGCACAG TCCCTGGAGC GCAGCAAGCA GCTGGACCAG CAGGCGCTCC 
AGCCGGAACA GCCTGGGCCG GGCCCCCAGC CTGAAGCGTA GGAGCCCAAG CGGGGAGCGG 
AGGTCCCTGC TGTCTGGAGA GGGTCAGGAG AGCCAGGATG AGGAGGAGAG TTCAGAAGAG 
GACCGGGCCA GCCCAGCAGG CAGTGATCAT CGCCACAGGG GTTCCTTGGA ACGTGAGGCC 
AAGAGTTCCT TTGACCTGCC TGACACCCTG CAGGTGCCCG GGCTTCATCG AACAGCCAGC 
GGTCGGAGCT CTGCCTCTGA ACACCAAGAC TGTAATGGCA AGTCGGCTTC AGGGCGTTTG 
GCCCGCACCC TGCGGGCTGA TGACCCCCCA CTGGATGGGG ATGATGGCGA TGATGAGGGC 
AACCTGAGCA AAGGGGAACG CTTACGAGCC TGGGTCCGAG CCCGGCTCCC TGCCTGTTGC 
CGAGAGCGAG ATTCCTGGTC TGCCTATATC TTCCCTCCCC AGTCAAGGTT TCGTCTCCTG 
TGTCACCGGA TCATCACCCA CAAGATGTTT GACCATGTGG TCCTCGTCAT CATCTTCCTC 
AACTGTATCA CCATCGCTAT GGAGCGCCCC AAAATTGACC CCCACAGCGC TGAACGCATC 
TTCCTGACCC TCTCCAACTA CATCTTCACG GCAGTCTTCC TGGCTGAAAT GACAGTGAAG 
GTGGTGGCAC TGGGCTGGTG CTTTGGGGAG CAGGCCTACC TGCGCAGCAG CTGGAACGTG 
CTGGACGGCT TGCTGGTGCT CATCTCTGTC ATCGACATCC TGGTGTCCAT GGTCTCTGAC 
AGCGGCACCA AGATTCTCGG CATGCTGAGG GTGCTGCGGC TGCTGCGGAC CCTACGTCCA 
CTCAGGGTCA TCAGCCGGGC CCAGGGGCTG AAGCTGGTGG TAGAGACTCT GATGTCATCC 
CTCAAACCCA TTGGCAACAT TGTGGTCATC TGCTGTGCCT TCTTCATCAT TTTTGGAATT 
CTTGGGGTGC AGCTCTTCAA AGGGAAGTTC TTCGTGTGTC AGGGTGAGGA CACCAGGAAC 
ATCACTAACA AGTCCGACTG TGCTGAGGCC AGTTACCGGT GGGTCCGGCA CAAGTACAAC 
TTTGACAACC TGGGCCAGGC TCTGATGTCC CTGTTTGTGC TGGCCTCCAA GGATGGCTGG 
GTTGACATCA TGTATGATGG ACTGGATGCT GTGGGAGTGG ACCAGCAGCC CATCATGAAC 
CACAACCCTT GGATGCTGCT CTACTTCATC TCCTTCCTCC TCATCGTGGC CTTCTTCGTC 
CTGAACATGT TTGTGGGCGT GGTGGTGGAG AACTTCCATA AGTGCAGGCA GCACCAGGAG 
GAGGAGGAGG CGCGGCGGCG GGAGGAGAAG CGACTAAAGA GGCTGGAGAA AAAGAGAAGG 
AGTAAGGAGA AGCAGATGGC CGATCTAATG TTGGACGATG TAATTGCTTC CGGCAGCTCA 
GCCAGCGCTG CGTCAGAAGC CCAGTGCAAA CCCTACTACT CTGACTACTC GCGCTTCCGG 
CTCCTCGTCC ACCACCTGTG TACCAGCCAC TACCTGGACC TCTTCATCAC TGGTGTCATC 
GGGCTGAATG TGGTCACGAT GGCCATGGAA CATTACCAGC AGCCCCAGAT CCTGGACGAG 
GCTCTGAAGA TCTGCAACTA CATCTTTACC GTCATCTTTG TCTTGGAGTC AGTATTCAAA 
CTTGTGGCCT TCGGCTTCCG CCGGTTCTTC CAGGACAGGT GGAACCAGCT GGACCTGGCT 
ATTGTGCTTC TGTCCATCAT GGGCATCACG CTGGAAGAGA TTGAGGTCAA TGCTTCACTG 
CCCATCAACC CCACCATCAT CCGTATCATG AGGGTGCTCC GCATTGCTCG AGTTCTGAAG 
CTGTTGAAGA TGGCTGTGGG CATGCGGGCA CTGCTGGACA CGGTGATGCA GGCCCTGCCC 
CAGGTGGGGA ACCTGGGACT TCTCTTCATG CTATTATTTT TCATCTTTGC AGCTCTGGGC 
GTGGAGCTCT TTGGAGACCT GGAGTGTGAT GAGACACACC CTTGTGAGGG CTTGGGCCGG 
CATGCCACCT TTAGGAACTT TGGTATGGCC TTTCTGACCC TCTTCCGAGT CTCCACTGGT 
GACAACTGGA ATGGTATTAT GAAGGACACC CTCCGGGACT GTGACCAGGA GTCCACCTGC 
TACAACACCG TCATCTCACC CATCTACTTC GTGTCCTTCG TGCTGACGGC CCAGTTTGTG 
CTGGTCAACG TGGTCATAGC CGTGCTGATG AAGCACCTGG AAGAGAGCAA CAAAGAGGCC 
AAGGAGGAGG CGGAGTTGGA GGCGGAGCTG GAGCTAGAGA TGAAGACACT CAGCCCGCAG 
CCCCACTCCC CGCTGGGCAG CCCCTTCCTC TGGCCTGGGG TGGAAGGTGT CAATAGCCCT 
GACAGCCCTA AGCCTGGGGC TCCACACACC ACGGCCCACA TTGGAGCAGC CTCTTCAGGC 
TTCTCCCTTG AGCACCCCAC GGACAGACAG CTGTTTGACA CCATCTCCCT GCTGATCCAG 
GGCTCCCTGG AGGGGGAGCT GAAGCTGATG GACGAGCTGG CAGGCCCAGG GGGCCAGCCC 
TCTGCCTTCC CTTCCGCCCC CAGCCCGGGC GACTCCGACC CACAGATCCC TCTAGCTGAG 
ATGGAGGCTC TGTCTCTGAC GTCAGAGATT GTGTCTGAAC CGTCCTGCTC TCTAGCTCTG 
ACGGATGACT CTTTGCCTGA TGACACTCAC ACACTCTTAC TTAGTGCCCT GGAGAGCAAT 
ATGGTACCTC ACACTGAGGA GGGGCCAGTC CCCCTAGGAC CAGACCTGCT GACTGTGAGG 
AAGTCTGGTG TCAGCCGGAC ACACTCTCTG CCCAATGACA GCTACATGTG CCGCAATGGG 
AGCACTGCCG AGAGATCCCT AGGACACAGG GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC 
TCCATCTTGT CTGTTCACTC CCAACCAGCA GACACCAGCT GCATCCTACA GCTTCCCAAA 
GATGCACACT ATCTGCTCCA GCCTCATGGG GCTCCCACCT GGGGCGCCAT CCCTAAACTA 
CCCCCACCTG GCCGCTCCCC TCTGGCTCAG AGGCCTCTCA GGCGCCAGGC AGCAATAAGG 
ACTGACTCCC TGGACGTGCA GGGCCTGGGT AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT 
GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA TCCTTCTGGG GCGGGTCGAG CATCCAGGTG 
CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC TCCAAGCACA TCCGCCTGCC AGCCCCTTGC 
CCAGGCCTGG AACCCAGCTG GGCCAAGGAC CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA GCAGTCAGGA AGAACCCCTG 
TCCCCACGGG ACTTGAAAAA ATGCTACAGT GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT 
GGGTCCTGGC TAGACGAACA GAGGAGACAC TCCATCGCTG TCAGCTGCCT GGACAGCGGC 
TCCCAGCCCC GCCTATGTCC AAGCCCCTCA AGCCTCGGGG GCCAACCTCT TGGGGGCCCT 
GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA CCCAGTATCT CTATAGACCC CCCGGAGAGC 
CAGGGCCCTC GGCCCCCATG CAGTCCTGGC GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT 
GACTCGAAGG ATCCCTCGGC CTCCAGCCCC CTTGACAGCA CGGCTGCCTC ACCCTCCCCA 
AAGAAAGATG CGCTGAGTCT CTCTGGTTTG TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248699.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247001.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X36, mRNA.

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CloneID	OMu67281
Clone ID Related Accession (Same CDS sequence)	XM_011248699.3 , XM_011248699.2
Accession Version	XM_011248699.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248699.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GGGACAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247001.1
CDS	773..5524
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAGTG

Target ORF information:

RefSeq Version	XM_011248699.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248699.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GGGACAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248700.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247002.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X37, mRNA.

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CloneID	OMu67282
Clone ID Related Accession (Same CDS sequence)	XM_011248700.2 , XM_011248700.3
Accession Version	XM_011248700.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248700.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GGACAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247002.1
CDS	773..5503
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRRTG

Target ORF information:

RefSeq Version	XM_011248700.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248700.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GGACAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532069.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532132.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X2, mRNA.

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CloneID	OMu45464
Clone ID Related Accession (Same CDS sequence)	XM_006532069.3 , XM_006532069.4
Accession Version	XM_006532069.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532069.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA TCTAATGTTG 
GACGATGTAA TTGCTTCCGG CAGCTCAGCC AGCGCTGCGT CAGAAGCCCA GTGCAAACCC 
TACTACTCTG ACTACTCGCG CTTCCGGCTC CTCGTCCACC ACCTGTGTAC CAGCCACTAC 
CTGGACCTCT TCATCACTGG TGTCATCGGG CTGAATGTGG TCACGATGGC CATGGAACAT 
TACCAGCAGC CCCAGATCCT GGACGAGGCT CTGAAGATCT GCAACTACAT CTTTACCGTC 
ATCTTTGTCT TGGAGTCAGT ATTCAAACTT GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCTATT GTGCTTCTGT CCATCATGGG CATCACGCTG 
GAAGAGATTG AGGTCAATGC TTCACTGCCC ATCAACCCCA CCATCATCCG TATCATGAGG 
GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG TTGAAGATGG CTGTGGGCAT GCGGGCACTG 
CTGGACACGG TGATGCAGGC CCTGCCCCAG GTGGGGAACC TGGGACTTCT CTTCATGCTA 
TTATTTTTCA TCTTTGCAGC TCTGGGCGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACACACCCTT GTGAGGGCTT GGGCCGGCAT GCCACCTTTA GGAACTTTGG TATGGCCTTT 
CTGACCCTCT TCCGAGTCTC CACTGGTGAC AACTGGAATG GTATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACCTGCTAC AACACCGTCA TCTCACCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TCATAGCCGT GCTGATGAAG 
CACCTGGAAG AGAGCAACAA AGAGGCCAAG GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG 
CTAGAGATGA AGACACTCAG CCCGCAGCCC CACTCCCCGC TGGGCAGCCC CTTCCTCTGG 
CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC AGCCCTAAGC CTGGGGCTCC ACACACCACG 
GCCCACATTG GAGCAGCCTC TTCAGGCTTC TCCCTTGAGC ACCCCACGGA CAGACAGCTG 
TTTGACACCA TCTCCCTGCT GATCCAGGGC TCCCTGGAGG GGGAGCTGAA GCTGATGGAC 
GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT GCCTTCCCTT CCGCCCCCAG CCCGGGCGAC 
TCCGACCCAC AGATCCCTCT AGCTGAGATG GAGGCTCTGT CTCTGACGTC AGAGATTGTG 
TCTGAACCGT CCTGCTCTCT AGCTCTGACG GATGACTCTT TGCCTGATGA CACTCACACA 
CTCTTACTTA GTGCCCTGGA GAGCAATATG GTACCTCACA CTGAGGAGGG GCCAGTCCCC 
CTAGGACCAG ACCTGCTGAC TGTGAGGAAG TCTGGTGTCA GCCGGACACA CTCTCTGCCC 
AATGACAGCT ACATGTGCCG CAATGGGAGC ACTGCCGAGA GATCCCTAGG ACACAGGGGC 
TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC ATCTTGTCTG TTCACTCCCA ACCAGCAGAC 
ACCAGCTGCA TCCTACAGCT TCCCAAAGAT GCACACTATC TGCTCCAGCC TCATGGGGCT 
CCCACCTGGG GCGCCATCCC TAAACTACCC CCACCTGGCC GCTCCCCTCT GGCTCAGAGG 
CCTCTCAGGC GCCAGGCAGC AATAAGGACT GACTCCCTGG ACGTGCAGGG CCTGGGTAGC 
CGGGAAGACC TGTTGTCAGA GGTGAGTGGG CCCTCCTGCC CTCTGACCCG CTCCTCATCC 
TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC 
AAGCACATCC GCCTGCCAGC CCCTTGCCCA GGCCTGGAAC CCAGCTGGGC CAAGGACCCT 
CAAGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC CCACGGGACT TGAAAAAATG CTACAGTGTA 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG TCCTGGCTAG ACGAACAGAG GAGACACTCC 
ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC CAGCCCCGCC TATGTCCAAG CCCCTCAAGC 
CTCGGGGGCC AACCTCTTGG GGGCCCTGGG AGCCGGCCTA AGAAAAAACT CAGCCCACCC 
AGTATCTCTA TAGACCCCCC GGAGAGCCAG GGCCCTCGGC CCCCATGCAG TCCTGGCGTC 
TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC TCGAAGGATC CCTCGGCCTC CAGCCCCCTT 
GACAGCACGG CTGCCTCACC CTCCCCAAAG AAAGATGCGC TGAGTCTCTC TGGTTTGTCT 
TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532132.1
CDS	784..7950
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532069.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532069.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA TCTAATGTTG 
GACGATGTAA TTGCTTCCGG CAGCTCAGCC AGCGCTGCGT CAGAAGCCCA GTGCAAACCC 
TACTACTCTG ACTACTCGCG CTTCCGGCTC CTCGTCCACC ACCTGTGTAC CAGCCACTAC 
CTGGACCTCT TCATCACTGG TGTCATCGGG CTGAATGTGG TCACGATGGC CATGGAACAT 
TACCAGCAGC CCCAGATCCT GGACGAGGCT CTGAAGATCT GCAACTACAT CTTTACCGTC 
ATCTTTGTCT TGGAGTCAGT ATTCAAACTT GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCTATT GTGCTTCTGT CCATCATGGG CATCACGCTG 
GAAGAGATTG AGGTCAATGC TTCACTGCCC ATCAACCCCA CCATCATCCG TATCATGAGG 
GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG TTGAAGATGG CTGTGGGCAT GCGGGCACTG 
CTGGACACGG TGATGCAGGC CCTGCCCCAG GTGGGGAACC TGGGACTTCT CTTCATGCTA 
TTATTTTTCA TCTTTGCAGC TCTGGGCGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACACACCCTT GTGAGGGCTT GGGCCGGCAT GCCACCTTTA GGAACTTTGG TATGGCCTTT 
CTGACCCTCT TCCGAGTCTC CACTGGTGAC AACTGGAATG GTATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACCTGCTAC AACACCGTCA TCTCACCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TCATAGCCGT GCTGATGAAG 
CACCTGGAAG AGAGCAACAA AGAGGCCAAG GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG 
CTAGAGATGA AGACACTCAG CCCGCAGCCC CACTCCCCGC TGGGCAGCCC CTTCCTCTGG 
CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC AGCCCTAAGC CTGGGGCTCC ACACACCACG 
GCCCACATTG GAGCAGCCTC TTCAGGCTTC TCCCTTGAGC ACCCCACGGA CAGACAGCTG 
TTTGACACCA TCTCCCTGCT GATCCAGGGC TCCCTGGAGG GGGAGCTGAA GCTGATGGAC 
GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT GCCTTCCCTT CCGCCCCCAG CCCGGGCGAC 
TCCGACCCAC AGATCCCTCT AGCTGAGATG GAGGCTCTGT CTCTGACGTC AGAGATTGTG 
TCTGAACCGT CCTGCTCTCT AGCTCTGACG GATGACTCTT TGCCTGATGA CACTCACACA 
CTCTTACTTA GTGCCCTGGA GAGCAATATG GTACCTCACA CTGAGGAGGG GCCAGTCCCC 
CTAGGACCAG ACCTGCTGAC TGTGAGGAAG TCTGGTGTCA GCCGGACACA CTCTCTGCCC 
AATGACAGCT ACATGTGCCG CAATGGGAGC ACTGCCGAGA GATCCCTAGG ACACAGGGGC 
TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC ATCTTGTCTG TTCACTCCCA ACCAGCAGAC 
ACCAGCTGCA TCCTACAGCT TCCCAAAGAT GCACACTATC TGCTCCAGCC TCATGGGGCT 
CCCACCTGGG GCGCCATCCC TAAACTACCC CCACCTGGCC GCTCCCCTCT GGCTCAGAGG 
CCTCTCAGGC GCCAGGCAGC AATAAGGACT GACTCCCTGG ACGTGCAGGG CCTGGGTAGC 
CGGGAAGACC TGTTGTCAGA GGTGAGTGGG CCCTCCTGCC CTCTGACCCG CTCCTCATCC 
TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC 
AAGCACATCC GCCTGCCAGC CCCTTGCCCA GGCCTGGAAC CCAGCTGGGC CAAGGACCCT 
CAAGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC CCACGGGACT TGAAAAAATG CTACAGTGTA 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG TCCTGGCTAG ACGAACAGAG GAGACACTCC 
ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC CAGCCCCGCC TATGTCCAAG CCCCTCAAGC 
CTCGGGGGCC AACCTCTTGG GGGCCCTGGG AGCCGGCCTA AGAAAAAACT CAGCCCACCC 
AGTATCTCTA TAGACCCCCC GGAGAGCCAG GGCCCTCGGC CCCCATGCAG TCCTGGCGTC 
TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC TCGAAGGATC CCTCGGCCTC CAGCCCCCTT 
GACAGCACGG CTGCCTCACC CTCCCCAAAG AAAGATGCGC TGAGTCTCTC TGGTTTGTCT 
TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532070.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532133.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X4, mRNA.

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CloneID	OMu45465
Clone ID Related Accession (Same CDS sequence)	XM_006532070.3 , XM_006532070.4
Accession Version	XM_006532070.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532070.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAT CTAATGTTGG ACGATGTAAT TGCTTCCGGC 
AGCTCAGCCA GCGCTGCGTC AGAAGCCCAG TGCAAACCCT ACTACTCTGA CTACTCGCGC 
TTCCGGCTCC TCGTCCACCA CCTGTGTACC AGCCACTACC TGGACCTCTT CATCACTGGT 
GTCATCGGGC TGAATGTGGT CACGATGGCC ATGGAACATT ACCAGCAGCC CCAGATCCTG 
GACGAGGCTC TGAAGATCTG CAACTACATC TTTACCGTCA TCTTTGTCTT GGAGTCAGTA 
TTCAAACTTG TGGCCTTCGG CTTCCGCCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCTATTG TGCTTCTGTC CATCATGGGC ATCACGCTGG AAGAGATTGA GGTCAATGCT 
TCACTGCCCA TCAACCCCAC CATCATCCGT ATCATGAGGG TGCTCCGCAT TGCTCGAGTT 
CTGAAGCTGT TGAAGATGGC TGTGGGCATG CGGGCACTGC TGGACACGGT GATGCAGGCC 
CTGCCCCAGG TGGGGAACCT GGGACTTCTC TTCATGCTAT TATTTTTCAT CTTTGCAGCT 
CTGGGCGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CACACCCTTG TGAGGGCTTG 
GGCCGGCATG CCACCTTTAG GAACTTTGGT ATGGCCTTTC TGACCCTCTT CCGAGTCTCC 
ACTGGTGACA ACTGGAATGG TATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACCTGCTACA ACACCGTCAT CTCACCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT CATAGCCGTG CTGATGAAGC ACCTGGAAGA GAGCAACAAA 
GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG GAGCTGGAGC TAGAGATGAA GACACTCAGC 
CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT 
AGCCCTGACA GCCCTAAGCC TGGGGCTCCA CACACCACGG CCCACATTGG AGCAGCCTCT 
TCAGGCTTCT CCCTTGAGCA CCCCACGGAC AGACAGCTGT TTGACACCAT CTCCCTGCTG 
ATCCAGGGCT CCCTGGAGGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTTC CGCCCCCAGC CCGGGCGACT CCGACCCACA GATCCCTCTA 
GCTGAGATGG AGGCTCTGTC TCTGACGTCA GAGATTGTGT CTGAACCGTC CTGCTCTCTA 
GCTCTGACGG ATGACTCTTT GCCTGATGAC ACTCACACAC TCTTACTTAG TGCCCTGGAG 
AGCAATATGG TACCTCACAC TGAGGAGGGG CCAGTCCCCC TAGGACCAGA CCTGCTGACT 
GTGAGGAAGT CTGGTGTCAG CCGGACACAC TCTCTGCCCA ATGACAGCTA CATGTGCCGC 
AATGGGAGCA CTGCCGAGAG ATCCCTAGGA CACAGGGGCT GGGGGCTCCC CAAAGCCCAG 
TCAGGCTCCA TCTTGTCTGT TCACTCCCAA CCAGCAGACA CCAGCTGCAT CCTACAGCTT 
CCCAAAGATG CACACTATCT GCTCCAGCCT CATGGGGCTC CCACCTGGGG CGCCATCCCT 
AAACTACCCC CACCTGGCCG CTCCCCTCTG GCTCAGAGGC CTCTCAGGCG CCAGGCAGCA 
ATAAGGACTG ACTCCCTGGA CGTGCAGGGC CTGGGTAGCC GGGAAGACCT GTTGTCAGAG 
GTGAGTGGGC CCTCCTGCCC TCTGACCCGC TCCTCATCCT TCTGGGGCGG GTCGAGCATC 
CAGGTGCAGC AGCGCTCCGG CAGCCAGAGC AAAGTCTCCA AGCACATCCG CCTGCCAGCC 
CCTTGCCCAG GCCTGGAACC CAGCTGGGCC AAGGACCCTC AAGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCAGCAG TCAGGAAGAA 
CCCCTGTCCC CACGGGACTT GAAAAAATGC TACAGTGTAG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGGGT CCTGGCTAGA CGAACAGAGG AGACACTCCA TCGCTGTCAG CTGCCTGGAC 
AGCGGCTCCC AGCCCCGCCT ATGTCCAAGC CCCTCAAGCC TCGGGGGCCA ACCTCTTGGG 
GGCCCTGGGA GCCGGCCTAA GAAAAAACTC AGCCCACCCA GTATCTCTAT AGACCCCCCG 
GAGAGCCAGG GCCCTCGGCC CCCATGCAGT CCTGGCGTCT GCCTCAGGAG GAGGGCGCCG 
GCCAGTGACT CGAAGGATCC CTCGGCCTCC AGCCCCCTTG ACAGCACGGC TGCCTCACCC 
TCCCCAAAGA AAGATGCGCT GAGTCTCTCT GGTTTGTCTT CTGACCCAAC AGACCTGGAT 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532133.1
CDS	784..7929
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532070.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532070.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAT CTAATGTTGG ACGATGTAAT TGCTTCCGGC 
AGCTCAGCCA GCGCTGCGTC AGAAGCCCAG TGCAAACCCT ACTACTCTGA CTACTCGCGC 
TTCCGGCTCC TCGTCCACCA CCTGTGTACC AGCCACTACC TGGACCTCTT CATCACTGGT 
GTCATCGGGC TGAATGTGGT CACGATGGCC ATGGAACATT ACCAGCAGCC CCAGATCCTG 
GACGAGGCTC TGAAGATCTG CAACTACATC TTTACCGTCA TCTTTGTCTT GGAGTCAGTA 
TTCAAACTTG TGGCCTTCGG CTTCCGCCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCTATTG TGCTTCTGTC CATCATGGGC ATCACGCTGG AAGAGATTGA GGTCAATGCT 
TCACTGCCCA TCAACCCCAC CATCATCCGT ATCATGAGGG TGCTCCGCAT TGCTCGAGTT 
CTGAAGCTGT TGAAGATGGC TGTGGGCATG CGGGCACTGC TGGACACGGT GATGCAGGCC 
CTGCCCCAGG TGGGGAACCT GGGACTTCTC TTCATGCTAT TATTTTTCAT CTTTGCAGCT 
CTGGGCGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CACACCCTTG TGAGGGCTTG 
GGCCGGCATG CCACCTTTAG GAACTTTGGT ATGGCCTTTC TGACCCTCTT CCGAGTCTCC 
ACTGGTGACA ACTGGAATGG TATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACCTGCTACA ACACCGTCAT CTCACCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT CATAGCCGTG CTGATGAAGC ACCTGGAAGA GAGCAACAAA 
GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG GAGCTGGAGC TAGAGATGAA GACACTCAGC 
CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT 
AGCCCTGACA GCCCTAAGCC TGGGGCTCCA CACACCACGG CCCACATTGG AGCAGCCTCT 
TCAGGCTTCT CCCTTGAGCA CCCCACGGAC AGACAGCTGT TTGACACCAT CTCCCTGCTG 
ATCCAGGGCT CCCTGGAGGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTTC CGCCCCCAGC CCGGGCGACT CCGACCCACA GATCCCTCTA 
GCTGAGATGG AGGCTCTGTC TCTGACGTCA GAGATTGTGT CTGAACCGTC CTGCTCTCTA 
GCTCTGACGG ATGACTCTTT GCCTGATGAC ACTCACACAC TCTTACTTAG TGCCCTGGAG 
AGCAATATGG TACCTCACAC TGAGGAGGGG CCAGTCCCCC TAGGACCAGA CCTGCTGACT 
GTGAGGAAGT CTGGTGTCAG CCGGACACAC TCTCTGCCCA ATGACAGCTA CATGTGCCGC 
AATGGGAGCA CTGCCGAGAG ATCCCTAGGA CACAGGGGCT GGGGGCTCCC CAAAGCCCAG 
TCAGGCTCCA TCTTGTCTGT TCACTCCCAA CCAGCAGACA CCAGCTGCAT CCTACAGCTT 
CCCAAAGATG CACACTATCT GCTCCAGCCT CATGGGGCTC CCACCTGGGG CGCCATCCCT 
AAACTACCCC CACCTGGCCG CTCCCCTCTG GCTCAGAGGC CTCTCAGGCG CCAGGCAGCA 
ATAAGGACTG ACTCCCTGGA CGTGCAGGGC CTGGGTAGCC GGGAAGACCT GTTGTCAGAG 
GTGAGTGGGC CCTCCTGCCC TCTGACCCGC TCCTCATCCT TCTGGGGCGG GTCGAGCATC 
CAGGTGCAGC AGCGCTCCGG CAGCCAGAGC AAAGTCTCCA AGCACATCCG CCTGCCAGCC 
CCTTGCCCAG GCCTGGAACC CAGCTGGGCC AAGGACCCTC AAGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCAGCAG TCAGGAAGAA 
CCCCTGTCCC CACGGGACTT GAAAAAATGC TACAGTGTAG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGGGT CCTGGCTAGA CGAACAGAGG AGACACTCCA TCGCTGTCAG CTGCCTGGAC 
AGCGGCTCCC AGCCCCGCCT ATGTCCAAGC CCCTCAAGCC TCGGGGGCCA ACCTCTTGGG 
GGCCCTGGGA GCCGGCCTAA GAAAAAACTC AGCCCACCCA GTATCTCTAT AGACCCCCCG 
GAGAGCCAGG GCCCTCGGCC CCCATGCAGT CCTGGCGTCT GCCTCAGGAG GAGGGCGCCG 
GCCAGTGACT CGAAGGATCC CTCGGCCTCC AGCCCCCTTG ACAGCACGGC TGCCTCACCC 
TCCCCAAAGA AAGATGCGCT GAGTCTCTCT GGTTTGTCTT CTGACCCAAC AGACCTGGAT 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532072.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532135.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X7, mRNA.

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CloneID	OMu45467
Clone ID Related Accession (Same CDS sequence)	XM_006532072.4 , XM_006532072.3
Accession Version	XM_006532072.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532072.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA AGCCCAGTGC 
AAACCCTACT ACTCTGACTA CTCGCGCTTC CGGCTCCTCG TCCACCACCT GTGTACCAGC 
CACTACCTGG ACCTCTTCAT CACTGGTGTC ATCGGGCTGA ATGTGGTCAC GATGGCCATG 
GAACATTACC AGCAGCCCCA GATCCTGGAC GAGGCTCTGA AGATCTGCAA CTACATCTTT 
ACCGTCATCT TTGTCTTGGA GTCAGTATTC AAACTTGTGG CCTTCGGCTT CCGCCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCTATTGTGC TTCTGTCCAT CATGGGCATC 
ACGCTGGAAG AGATTGAGGT CAATGCTTCA CTGCCCATCA ACCCCACCAT CATCCGTATC 
ATGAGGGTGC TCCGCATTGC TCGAGTTCTG AAGCTGTTGA AGATGGCTGT GGGCATGCGG 
GCACTGCTGG ACACGGTGAT GCAGGCCCTG CCCCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGCTATTAT TTTTCATCTT TGCAGCTCTG GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACAC ACCCTTGTGA GGGCTTGGGC CGGCATGCCA CCTTTAGGAA CTTTGGTATG 
GCCTTTCTGA CCCTCTTCCG AGTCTCCACT GGTGACAACT GGAATGGTAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACC TGCTACAACA CCGTCATCTC ACCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG 
ATGAAGCACC TGGAAGAGAG CAACAAAGAG GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG 
CTGGAGCTAG AGATGAAGAC ACTCAGCCCG CAGCCCCACT CCCCGCTGGG CAGCCCCTTC 
CTCTGGCCTG GGGTGGAAGG TGTCAATAGC CCTGACAGCC CTAAGCCTGG GGCTCCACAC 
ACCACGGCCC ACATTGGAGC AGCCTCTTCA GGCTTCTCCC TTGAGCACCC CACGGACAGA 
CAGCTGTTTG ACACCATCTC CCTGCTGATC CAGGGCTCCC TGGAGGGGGA GCTGAAGCTG 
ATGGACGAGC TGGCAGGCCC AGGGGGCCAG CCCTCTGCCT TCCCTTCCGC CCCCAGCCCG 
GGCGACTCCG ACCCACAGAT CCCTCTAGCT GAGATGGAGG CTCTGTCTCT GACGTCAGAG 
ATTGTGTCTG AACCGTCCTG CTCTCTAGCT CTGACGGATG ACTCTTTGCC TGATGACACT 
CACACACTCT TACTTAGTGC CCTGGAGAGC AATATGGTAC CTCACACTGA GGAGGGGCCA 
GTCCCCCTAG GACCAGACCT GCTGACTGTG AGGAAGTCTG GTGTCAGCCG GACACACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGCAAT GGGAGCACTG CCGAGAGATC CCTAGGACAC 
AGGGGCTGGG GGCTCCCCAA AGCCCAGTCA GGCTCCATCT TGTCTGTTCA CTCCCAACCA 
GCAGACACCA GCTGCATCCT ACAGCTTCCC AAAGATGCAC ACTATCTGCT CCAGCCTCAT 
GGGGCTCCCA CCTGGGGCGC CATCCCTAAA CTACCCCCAC CTGGCCGCTC CCCTCTGGCT 
CAGAGGCCTC TCAGGCGCCA GGCAGCAATA AGGACTGACT CCCTGGACGT GCAGGGCCTG 
GGTAGCCGGG AAGACCTGTT GTCAGAGGTG AGTGGGCCCT CCTGCCCTCT GACCCGCTCC 
TCATCCTTCT GGGGCGGGTC GAGCATCCAG GTGCAGCAGC GCTCCGGCAG CCAGAGCAAA 
GTCTCCAAGC ACATCCGCCT GCCAGCCCCT TGCCCAGGCC TGGAACCCAG CTGGGCCAAG 
GACCCTCAAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCAGCAGTCA GGAAGAACCC CTGTCCCCAC GGGACTTGAA AAAATGCTAC 
AGTGTAGAGG CCCAGAGCTG CCGGCGCAGG CCTGGGTCCT GGCTAGACGA ACAGAGGAGA 
CACTCCATCG CTGTCAGCTG CCTGGACAGC GGCTCCCAGC CCCGCCTATG TCCAAGCCCC 
TCAAGCCTCG GGGGCCAACC TCTTGGGGGC CCTGGGAGCC GGCCTAAGAA AAAACTCAGC 
CCACCCAGTA TCTCTATAGA CCCCCCGGAG AGCCAGGGCC CTCGGCCCCC ATGCAGTCCT 
GGCGTCTGCC TCAGGAGGAG GGCGCCGGCC AGTGACTCGA AGGATCCCTC GGCCTCCAGC 
CCCCTTGACA GCACGGCTGC CTCACCCTCC CCAAAGAAAG ATGCGCTGAG TCTCTCTGGT 
TTGTCTTCTG ACCCAACAGA CCTGGATCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532135.1
CDS	784..7896
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532072.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532072.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA AGCCCAGTGC 
AAACCCTACT ACTCTGACTA CTCGCGCTTC CGGCTCCTCG TCCACCACCT GTGTACCAGC 
CACTACCTGG ACCTCTTCAT CACTGGTGTC ATCGGGCTGA ATGTGGTCAC GATGGCCATG 
GAACATTACC AGCAGCCCCA GATCCTGGAC GAGGCTCTGA AGATCTGCAA CTACATCTTT 
ACCGTCATCT TTGTCTTGGA GTCAGTATTC AAACTTGTGG CCTTCGGCTT CCGCCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCTATTGTGC TTCTGTCCAT CATGGGCATC 
ACGCTGGAAG AGATTGAGGT CAATGCTTCA CTGCCCATCA ACCCCACCAT CATCCGTATC 
ATGAGGGTGC TCCGCATTGC TCGAGTTCTG AAGCTGTTGA AGATGGCTGT GGGCATGCGG 
GCACTGCTGG ACACGGTGAT GCAGGCCCTG CCCCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGCTATTAT TTTTCATCTT TGCAGCTCTG GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACAC ACCCTTGTGA GGGCTTGGGC CGGCATGCCA CCTTTAGGAA CTTTGGTATG 
GCCTTTCTGA CCCTCTTCCG AGTCTCCACT GGTGACAACT GGAATGGTAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACC TGCTACAACA CCGTCATCTC ACCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG 
ATGAAGCACC TGGAAGAGAG CAACAAAGAG GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG 
CTGGAGCTAG AGATGAAGAC ACTCAGCCCG CAGCCCCACT CCCCGCTGGG CAGCCCCTTC 
CTCTGGCCTG GGGTGGAAGG TGTCAATAGC CCTGACAGCC CTAAGCCTGG GGCTCCACAC 
ACCACGGCCC ACATTGGAGC AGCCTCTTCA GGCTTCTCCC TTGAGCACCC CACGGACAGA 
CAGCTGTTTG ACACCATCTC CCTGCTGATC CAGGGCTCCC TGGAGGGGGA GCTGAAGCTG 
ATGGACGAGC TGGCAGGCCC AGGGGGCCAG CCCTCTGCCT TCCCTTCCGC CCCCAGCCCG 
GGCGACTCCG ACCCACAGAT CCCTCTAGCT GAGATGGAGG CTCTGTCTCT GACGTCAGAG 
ATTGTGTCTG AACCGTCCTG CTCTCTAGCT CTGACGGATG ACTCTTTGCC TGATGACACT 
CACACACTCT TACTTAGTGC CCTGGAGAGC AATATGGTAC CTCACACTGA GGAGGGGCCA 
GTCCCCCTAG GACCAGACCT GCTGACTGTG AGGAAGTCTG GTGTCAGCCG GACACACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGCAAT GGGAGCACTG CCGAGAGATC CCTAGGACAC 
AGGGGCTGGG GGCTCCCCAA AGCCCAGTCA GGCTCCATCT TGTCTGTTCA CTCCCAACCA 
GCAGACACCA GCTGCATCCT ACAGCTTCCC AAAGATGCAC ACTATCTGCT CCAGCCTCAT 
GGGGCTCCCA CCTGGGGCGC CATCCCTAAA CTACCCCCAC CTGGCCGCTC CCCTCTGGCT 
CAGAGGCCTC TCAGGCGCCA GGCAGCAATA AGGACTGACT CCCTGGACGT GCAGGGCCTG 
GGTAGCCGGG AAGACCTGTT GTCAGAGGTG AGTGGGCCCT CCTGCCCTCT GACCCGCTCC 
TCATCCTTCT GGGGCGGGTC GAGCATCCAG GTGCAGCAGC GCTCCGGCAG CCAGAGCAAA 
GTCTCCAAGC ACATCCGCCT GCCAGCCCCT TGCCCAGGCC TGGAACCCAG CTGGGCCAAG 
GACCCTCAAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCAGCAGTCA GGAAGAACCC CTGTCCCCAC GGGACTTGAA AAAATGCTAC 
AGTGTAGAGG CCCAGAGCTG CCGGCGCAGG CCTGGGTCCT GGCTAGACGA ACAGAGGAGA 
CACTCCATCG CTGTCAGCTG CCTGGACAGC GGCTCCCAGC CCCGCCTATG TCCAAGCCCC 
TCAAGCCTCG GGGGCCAACC TCTTGGGGGC CCTGGGAGCC GGCCTAAGAA AAAACTCAGC 
CCACCCAGTA TCTCTATAGA CCCCCCGGAG AGCCAGGGCC CTCGGCCCCC ATGCAGTCCT 
GGCGTCTGCC TCAGGAGGAG GGCGCCGGCC AGTGACTCGA AGGATCCCTC GGCCTCCAGC 
CCCCTTGACA GCACGGCTGC CTCACCCTCC CCAAAGAAAG ATGCGCTGAG TCTCTCTGGT 
TTGTCTTCTG ACCCAACAGA CCTGGATCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532073.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532136.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X10, mRNA.

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CloneID	OMu45468
Clone ID Related Accession (Same CDS sequence)	XM_006532073.3 , XM_006532073.4
Accession Version	XM_006532073.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532073.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG ATCTAATGTT GGACGATGTA 
ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG TCAGAAGCCC AGTGCAAACC CTACTACTCT 
GACTACTCGC GCTTCCGGCT CCTCGTCCAC CACCTGTGTA CCAGCCACTA CCTGGACCTC 
TTCATCACTG GTGTCATCGG GCTGAATGTG GTCACGATGG CCATGGAACA TTACCAGCAG 
CCCCAGATCC TGGACGAGGC TCTGAAGATC TGCAACTACA TCTTTACCGT CATCTTTGTC 
TTGGAGTCAG TATTCAAACT TGTGGCCTTC GGCTTCCGCC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCTAT TGTGCTTCTG TCCATCATGG GCATCACGCT GGAAGAGATT 
GAGGTCAATG CTTCACTGCC CATCAACCCC ACCATCATCC GTATCATGAG GGTGCTCCGC 
ATTGCTCGAG TTCTGAAGCT GTTGAAGATG GCTGTGGGCA TGCGGGCACT GCTGGACACG 
GTGATGCAGG CCCTGCCCCA GGTGGGGAAC CTGGGACTTC TCTTCATGCT ATTATTTTTC 
ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACACACCCT 
TGTGAGGGCT TGGGCCGGCA TGCCACCTTT AGGAACTTTG GTATGGCCTT TCTGACCCTC 
TTCCGAGTCT CCACTGGTGA CAACTGGAAT GGTATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACCTGCTA CAACACCGTC ATCTCACCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTCATAGCCG TGCTGATGAA GCACCTGGAA 
GAGAGCAACA AAGAGGCCAA GGAGGAGGCG GAGTTGGAGG CGGAGCTGGA GCTAGAGATG 
AAGACACTCA GCCCGCAGCC CCACTCCCCG CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG 
GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG CCTGGGGCTC CACACACCAC GGCCCACATT 
GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG CACCCCACGG ACAGACAGCT GTTTGACACC 
ATCTCCCTGC TGATCCAGGG CTCCCTGGAG GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT TCCGCCCCCA GCCCGGGCGA CTCCGACCCA 
CAGATCCCTC TAGCTGAGAT GGAGGCTCTG TCTCTGACGT CAGAGATTGT GTCTGAACCG 
TCCTGCTCTC TAGCTCTGAC GGATGACTCT TTGCCTGATG ACACTCACAC ACTCTTACTT 
AGTGCCCTGG AGAGCAATAT GGTACCTCAC ACTGAGGAGG GGCCAGTCCC CCTAGGACCA 
GACCTGCTGA CTGTGAGGAA GTCTGGTGTC AGCCGGACAC ACTCTCTGCC CAATGACAGC 
TACATGTGCC GCAATGGGAG CACTGCCGAG AGATCCCTAG GACACAGGGG CTGGGGGCTC 
CCCAAAGCCC AGTCAGGCTC CATCTTGTCT GTTCACTCCC AACCAGCAGA CACCAGCTGC 
ATCCTACAGC TTCCCAAAGA TGCACACTAT CTGCTCCAGC CTCATGGGGC TCCCACCTGG 
GGCGCCATCC CTAAACTACC CCCACCTGGC CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG 
CGCCAGGCAG CAATAAGGAC TGACTCCCTG GACGTGCAGG GCCTGGGTAG CCGGGAAGAC 
CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC CCTCTGACCC GCTCCTCATC CTTCTGGGGC 
GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC GGCAGCCAGA GCAAAGTCTC CAAGCACATC 
CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA CCCAGCTGGG CCAAGGACCC TCAAGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGCCCAGC 
AGTCAGGAAG AACCCCTGTC CCCACGGGAC TTGAAAAAAT GCTACAGTGT AGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA GACGAACAGA GGAGACACTC CATCGCTGTC 
AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC CTATGTCCAA GCCCCTCAAG CCTCGGGGGC 
CAACCTCTTG GGGGCCCTGG GAGCCGGCCT AAGAAAAAAC TCAGCCCACC CAGTATCTCT 
ATAGACCCCC CGGAGAGCCA GGGCCCTCGG CCCCCATGCA GTCCTGGCGT CTGCCTCAGG 
AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT CCCTCGGCCT CCAGCCCCCT TGACAGCACG 
GCTGCCTCAC CCTCCCCAAA GAAAGATGCG CTGAGTCTCT CTGGTTTGTC TTCTGACCCA 
ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532136.1
CDS	784..7881
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532073.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532073.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG ATCTAATGTT GGACGATGTA 
ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG TCAGAAGCCC AGTGCAAACC CTACTACTCT 
GACTACTCGC GCTTCCGGCT CCTCGTCCAC CACCTGTGTA CCAGCCACTA CCTGGACCTC 
TTCATCACTG GTGTCATCGG GCTGAATGTG GTCACGATGG CCATGGAACA TTACCAGCAG 
CCCCAGATCC TGGACGAGGC TCTGAAGATC TGCAACTACA TCTTTACCGT CATCTTTGTC 
TTGGAGTCAG TATTCAAACT TGTGGCCTTC GGCTTCCGCC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCTAT TGTGCTTCTG TCCATCATGG GCATCACGCT GGAAGAGATT 
GAGGTCAATG CTTCACTGCC CATCAACCCC ACCATCATCC GTATCATGAG GGTGCTCCGC 
ATTGCTCGAG TTCTGAAGCT GTTGAAGATG GCTGTGGGCA TGCGGGCACT GCTGGACACG 
GTGATGCAGG CCCTGCCCCA GGTGGGGAAC CTGGGACTTC TCTTCATGCT ATTATTTTTC 
ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACACACCCT 
TGTGAGGGCT TGGGCCGGCA TGCCACCTTT AGGAACTTTG GTATGGCCTT TCTGACCCTC 
TTCCGAGTCT CCACTGGTGA CAACTGGAAT GGTATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACCTGCTA CAACACCGTC ATCTCACCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTCATAGCCG TGCTGATGAA GCACCTGGAA 
GAGAGCAACA AAGAGGCCAA GGAGGAGGCG GAGTTGGAGG CGGAGCTGGA GCTAGAGATG 
AAGACACTCA GCCCGCAGCC CCACTCCCCG CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG 
GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG CCTGGGGCTC CACACACCAC GGCCCACATT 
GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG CACCCCACGG ACAGACAGCT GTTTGACACC 
ATCTCCCTGC TGATCCAGGG CTCCCTGGAG GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT TCCGCCCCCA GCCCGGGCGA CTCCGACCCA 
CAGATCCCTC TAGCTGAGAT GGAGGCTCTG TCTCTGACGT CAGAGATTGT GTCTGAACCG 
TCCTGCTCTC TAGCTCTGAC GGATGACTCT TTGCCTGATG ACACTCACAC ACTCTTACTT 
AGTGCCCTGG AGAGCAATAT GGTACCTCAC ACTGAGGAGG GGCCAGTCCC CCTAGGACCA 
GACCTGCTGA CTGTGAGGAA GTCTGGTGTC AGCCGGACAC ACTCTCTGCC CAATGACAGC 
TACATGTGCC GCAATGGGAG CACTGCCGAG AGATCCCTAG GACACAGGGG CTGGGGGCTC 
CCCAAAGCCC AGTCAGGCTC CATCTTGTCT GTTCACTCCC AACCAGCAGA CACCAGCTGC 
ATCCTACAGC TTCCCAAAGA TGCACACTAT CTGCTCCAGC CTCATGGGGC TCCCACCTGG 
GGCGCCATCC CTAAACTACC CCCACCTGGC CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG 
CGCCAGGCAG CAATAAGGAC TGACTCCCTG GACGTGCAGG GCCTGGGTAG CCGGGAAGAC 
CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC CCTCTGACCC GCTCCTCATC CTTCTGGGGC 
GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC GGCAGCCAGA GCAAAGTCTC CAAGCACATC 
CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA CCCAGCTGGG CCAAGGACCC TCAAGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGCCCAGC 
AGTCAGGAAG AACCCCTGTC CCCACGGGAC TTGAAAAAAT GCTACAGTGT AGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA GACGAACAGA GGAGACACTC CATCGCTGTC 
AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC CTATGTCCAA GCCCCTCAAG CCTCGGGGGC 
CAACCTCTTG GGGGCCCTGG GAGCCGGCCT AAGAAAAAAC TCAGCCCACC CAGTATCTCT 
ATAGACCCCC CGGAGAGCCA GGGCCCTCGG CCCCCATGCA GTCCTGGCGT CTGCCTCAGG 
AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT CCCTCGGCCT CCAGCCCCCT TGACAGCACG 
GCTGCCTCAC CCTCCCCAAA GAAAGATGCG CTGAGTCTCT CTGGTTTGTC TTCTGACCCA 
ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532074.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532137.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X11, mRNA.

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CloneID	OMu45469
Clone ID Related Accession (Same CDS sequence)	XM_006532074.3 , XM_006532074.4
Accession Version	XM_006532074.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532074.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAA GCCCAGTGCA AACCCTACTA CTCTGACTAC 
TCGCGCTTCC GGCTCCTCGT CCACCACCTG TGTACCAGCC ACTACCTGGA CCTCTTCATC 
ACTGGTGTCA TCGGGCTGAA TGTGGTCACG ATGGCCATGG AACATTACCA GCAGCCCCAG 
ATCCTGGACG AGGCTCTGAA GATCTGCAAC TACATCTTTA CCGTCATCTT TGTCTTGGAG 
TCAGTATTCA AACTTGTGGC CTTCGGCTTC CGCCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CTATTGTGCT TCTGTCCATC ATGGGCATCA CGCTGGAAGA GATTGAGGTC 
AATGCTTCAC TGCCCATCAA CCCCACCATC ATCCGTATCA TGAGGGTGCT CCGCATTGCT 
CGAGTTCTGA AGCTGTTGAA GATGGCTGTG GGCATGCGGG CACTGCTGGA CACGGTGATG 
CAGGCCCTGC CCCAGGTGGG GAACCTGGGA CTTCTCTTCA TGCTATTATT TTTCATCTTT 
GCAGCTCTGG GCGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACACA CCCTTGTGAG 
GGCTTGGGCC GGCATGCCAC CTTTAGGAAC TTTGGTATGG CCTTTCTGAC CCTCTTCCGA 
GTCTCCACTG GTGACAACTG GAATGGTATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACCT GCTACAACAC CGTCATCTCA CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTCATA GCCGTGCTGA TGAAGCACCT GGAAGAGAGC 
AACAAAGAGG CCAAGGAGGA GGCGGAGTTG GAGGCGGAGC TGGAGCTAGA GATGAAGACA 
CTCAGCCCGC AGCCCCACTC CCCGCTGGGC AGCCCCTTCC TCTGGCCTGG GGTGGAAGGT 
GTCAATAGCC CTGACAGCCC TAAGCCTGGG GCTCCACACA CCACGGCCCA CATTGGAGCA 
GCCTCTTCAG GCTTCTCCCT TGAGCACCCC ACGGACAGAC AGCTGTTTGA CACCATCTCC 
CTGCTGATCC AGGGCTCCCT GGAGGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA 
GGGGGCCAGC CCTCTGCCTT CCCTTCCGCC CCCAGCCCGG GCGACTCCGA CCCACAGATC 
CCTCTAGCTG AGATGGAGGC TCTGTCTCTG ACGTCAGAGA TTGTGTCTGA ACCGTCCTGC 
TCTCTAGCTC TGACGGATGA CTCTTTGCCT GATGACACTC ACACACTCTT ACTTAGTGCC 
CTGGAGAGCA ATATGGTACC TCACACTGAG GAGGGGCCAG TCCCCCTAGG ACCAGACCTG 
CTGACTGTGA GGAAGTCTGG TGTCAGCCGG ACACACTCTC TGCCCAATGA CAGCTACATG 
TGCCGCAATG GGAGCACTGC CGAGAGATCC CTAGGACACA GGGGCTGGGG GCTCCCCAAA 
GCCCAGTCAG GCTCCATCTT GTCTGTTCAC TCCCAACCAG CAGACACCAG CTGCATCCTA 
CAGCTTCCCA AAGATGCACA CTATCTGCTC CAGCCTCATG GGGCTCCCAC CTGGGGCGCC 
ATCCCTAAAC TACCCCCACC TGGCCGCTCC CCTCTGGCTC AGAGGCCTCT CAGGCGCCAG 
GCAGCAATAA GGACTGACTC CCTGGACGTG CAGGGCCTGG GTAGCCGGGA AGACCTGTTG 
TCAGAGGTGA GTGGGCCCTC CTGCCCTCTG ACCCGCTCCT CATCCTTCTG GGGCGGGTCG 
AGCATCCAGG TGCAGCAGCG CTCCGGCAGC CAGAGCAAAG TCTCCAAGCA CATCCGCCTG 
CCAGCCCCTT GCCCAGGCCT GGAACCCAGC TGGGCCAAGG ACCCTCAAGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CAGCAGTCAG 
GAAGAACCCC TGTCCCCACG GGACTTGAAA AAATGCTACA GTGTAGAGGC CCAGAGCTGC 
CGGCGCAGGC CTGGGTCCTG GCTAGACGAA CAGAGGAGAC ACTCCATCGC TGTCAGCTGC 
CTGGACAGCG GCTCCCAGCC CCGCCTATGT CCAAGCCCCT CAAGCCTCGG GGGCCAACCT 
CTTGGGGGCC CTGGGAGCCG GCCTAAGAAA AAACTCAGCC CACCCAGTAT CTCTATAGAC 
CCCCCGGAGA GCCAGGGCCC TCGGCCCCCA TGCAGTCCTG GCGTCTGCCT CAGGAGGAGG 
GCGCCGGCCA GTGACTCGAA GGATCCCTCG GCCTCCAGCC CCCTTGACAG CACGGCTGCC 
TCACCCTCCC CAAAGAAAGA TGCGCTGAGT CTCTCTGGTT TGTCTTCTGA CCCAACAGAC 
CTGGATCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532137.1
CDS	784..7875
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532074.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532074.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAA GCCCAGTGCA AACCCTACTA CTCTGACTAC 
TCGCGCTTCC GGCTCCTCGT CCACCACCTG TGTACCAGCC ACTACCTGGA CCTCTTCATC 
ACTGGTGTCA TCGGGCTGAA TGTGGTCACG ATGGCCATGG AACATTACCA GCAGCCCCAG 
ATCCTGGACG AGGCTCTGAA GATCTGCAAC TACATCTTTA CCGTCATCTT TGTCTTGGAG 
TCAGTATTCA AACTTGTGGC CTTCGGCTTC CGCCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CTATTGTGCT TCTGTCCATC ATGGGCATCA CGCTGGAAGA GATTGAGGTC 
AATGCTTCAC TGCCCATCAA CCCCACCATC ATCCGTATCA TGAGGGTGCT CCGCATTGCT 
CGAGTTCTGA AGCTGTTGAA GATGGCTGTG GGCATGCGGG CACTGCTGGA CACGGTGATG 
CAGGCCCTGC CCCAGGTGGG GAACCTGGGA CTTCTCTTCA TGCTATTATT TTTCATCTTT 
GCAGCTCTGG GCGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACACA CCCTTGTGAG 
GGCTTGGGCC GGCATGCCAC CTTTAGGAAC TTTGGTATGG CCTTTCTGAC CCTCTTCCGA 
GTCTCCACTG GTGACAACTG GAATGGTATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACCT GCTACAACAC CGTCATCTCA CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTCATA GCCGTGCTGA TGAAGCACCT GGAAGAGAGC 
AACAAAGAGG CCAAGGAGGA GGCGGAGTTG GAGGCGGAGC TGGAGCTAGA GATGAAGACA 
CTCAGCCCGC AGCCCCACTC CCCGCTGGGC AGCCCCTTCC TCTGGCCTGG GGTGGAAGGT 
GTCAATAGCC CTGACAGCCC TAAGCCTGGG GCTCCACACA CCACGGCCCA CATTGGAGCA 
GCCTCTTCAG GCTTCTCCCT TGAGCACCCC ACGGACAGAC AGCTGTTTGA CACCATCTCC 
CTGCTGATCC AGGGCTCCCT GGAGGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA 
GGGGGCCAGC CCTCTGCCTT CCCTTCCGCC CCCAGCCCGG GCGACTCCGA CCCACAGATC 
CCTCTAGCTG AGATGGAGGC TCTGTCTCTG ACGTCAGAGA TTGTGTCTGA ACCGTCCTGC 
TCTCTAGCTC TGACGGATGA CTCTTTGCCT GATGACACTC ACACACTCTT ACTTAGTGCC 
CTGGAGAGCA ATATGGTACC TCACACTGAG GAGGGGCCAG TCCCCCTAGG ACCAGACCTG 
CTGACTGTGA GGAAGTCTGG TGTCAGCCGG ACACACTCTC TGCCCAATGA CAGCTACATG 
TGCCGCAATG GGAGCACTGC CGAGAGATCC CTAGGACACA GGGGCTGGGG GCTCCCCAAA 
GCCCAGTCAG GCTCCATCTT GTCTGTTCAC TCCCAACCAG CAGACACCAG CTGCATCCTA 
CAGCTTCCCA AAGATGCACA CTATCTGCTC CAGCCTCATG GGGCTCCCAC CTGGGGCGCC 
ATCCCTAAAC TACCCCCACC TGGCCGCTCC CCTCTGGCTC AGAGGCCTCT CAGGCGCCAG 
GCAGCAATAA GGACTGACTC CCTGGACGTG CAGGGCCTGG GTAGCCGGGA AGACCTGTTG 
TCAGAGGTGA GTGGGCCCTC CTGCCCTCTG ACCCGCTCCT CATCCTTCTG GGGCGGGTCG 
AGCATCCAGG TGCAGCAGCG CTCCGGCAGC CAGAGCAAAG TCTCCAAGCA CATCCGCCTG 
CCAGCCCCTT GCCCAGGCCT GGAACCCAGC TGGGCCAAGG ACCCTCAAGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CAGCAGTCAG 
GAAGAACCCC TGTCCCCACG GGACTTGAAA AAATGCTACA GTGTAGAGGC CCAGAGCTGC 
CGGCGCAGGC CTGGGTCCTG GCTAGACGAA CAGAGGAGAC ACTCCATCGC TGTCAGCTGC 
CTGGACAGCG GCTCCCAGCC CCGCCTATGT CCAAGCCCCT CAAGCCTCGG GGGCCAACCT 
CTTGGGGGCC CTGGGAGCCG GCCTAAGAAA AAACTCAGCC CACCCAGTAT CTCTATAGAC 
CCCCCGGAGA GCCAGGGCCC TCGGCCCCCA TGCAGTCCTG GCGTCTGCCT CAGGAGGAGG 
GCGCCGGCCA GTGACTCGAA GGATCCCTCG GCCTCCAGCC CCCTTGACAG CACGGCTGCC 
TCACCCTCCC CAAAGAAAGA TGCGCTGAGT CTCTCTGGTT TGTCTTCTGA CCCAACAGAC 
CTGGATCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532075.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532138.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X12, mRNA.

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CloneID	OMu45470
Clone ID Related Accession (Same CDS sequence)	XM_006532075.3 , XM_006532075.4
Accession Version	XM_006532075.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532075.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAA TCTAATGTTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCTGCGT CAGAAGCCCA GTGCAAACCC TACTACTCTG ACTACTCGCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGACCTCT TCATCACTGG TGTCATCGGG 
CTGAATGTGG TCACGATGGC CATGGAACAT TACCAGCAGC CCCAGATCCT GGACGAGGCT 
CTGAAGATCT GCAACTACAT CTTTACCGTC ATCTTTGTCT TGGAGTCAGT ATTCAAACTT 
GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCTATT 
GTGCTTCTGT CCATCATGGG CATCACGCTG GAAGAGATTG AGGTCAATGC TTCACTGCCC 
ATCAACCCCA CCATCATCCG TATCATGAGG GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG 
TTGAAGATGG CTGTGGGCAT GCGGGCACTG CTGGACACGG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACC TGGGACTTCT CTTCATGCTA TTATTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGACCTGGA GTGTGATGAG ACACACCCTT GTGAGGGCTT GGGCCGGCAT 
GCCACCTTTA GGAACTTTGG TATGGCCTTT CTGACCCTCT TCCGAGTCTC CACTGGTGAC 
AACTGGAATG GTATTATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACCGTCA TCTCACCCAT CTACTTCGTG TCCTTCGTGC TGACGGCCCA GTTTGTGCTG 
GTCAACGTGG TCATAGCCGT GCTGATGAAG CACCTGGAAG AGAGCAACAA AGAGGCCAAG 
GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG CTAGAGATGA AGACACTCAG CCCGCAGCCC 
CACTCCCCGC TGGGCAGCCC CTTCCTCTGG CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC 
AGCCCTAAGC CTGGGGCTCC ACACACCACG GCCCACATTG GAGCAGCCTC TTCAGGCTTC 
TCCCTTGAGC ACCCCACGGA CAGACAGCTG TTTGACACCA TCTCCCTGCT GATCCAGGGC 
TCCCTGGAGG GGGAGCTGAA GCTGATGGAC GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT 
GCCTTCCCTT CCGCCCCCAG CCCGGGCGAC TCCGACCCAC AGATCCCTCT AGCTGAGATG 
GAGGCTCTGT CTCTGACGTC AGAGATTGTG TCTGAACCGT CCTGCTCTCT AGCTCTGACG 
GATGACTCTT TGCCTGATGA CACTCACACA CTCTTACTTA GTGCCCTGGA GAGCAATATG 
GTACCTCACA CTGAGGAGGG GCCAGTCCCC CTAGGACCAG ACCTGCTGAC TGTGAGGAAG 
TCTGGTGTCA GCCGGACACA CTCTCTGCCC AATGACAGCT ACATGTGCCG CAATGGGAGC 
ACTGCCGAGA GATCCCTAGG ACACAGGGGC TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC 
ATCTTGTCTG TTCACTCCCA ACCAGCAGAC ACCAGCTGCA TCCTACAGCT TCCCAAAGAT 
GCACACTATC TGCTCCAGCC TCATGGGGCT CCCACCTGGG GCGCCATCCC TAAACTACCC 
CCACCTGGCC GCTCCCCTCT GGCTCAGAGG CCTCTCAGGC GCCAGGCAGC AATAAGGACT 
GACTCCCTGG ACGTGCAGGG CCTGGGTAGC CGGGAAGACC TGTTGTCAGA GGTGAGTGGG 
CCCTCCTGCC CTCTGACCCG CTCCTCATCC TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG 
CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC AAGCACATCC GCCTGCCAGC CCCTTGCCCA 
GGCCTGGAAC CCAGCTGGGC CAAGGACCCT CAAGAGACCA GAAGCAGCTT AGAGCTGGAC 
ACGGAGCTGA GCTGGATTTC AGGAGACCTC CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC 
CCACGGGACT TGAAAAAATG CTACAGTGTA GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG 
TCCTGGCTAG ACGAACAGAG GAGACACTCC ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC 
CAGCCCCGCC TATGTCCAAG CCCCTCAAGC CTCGGGGGCC AACCTCTTGG GGGCCCTGGG 
AGCCGGCCTA AGAAAAAACT CAGCCCACCC AGTATCTCTA TAGACCCCCC GGAGAGCCAG 
GGCCCTCGGC CCCCATGCAG TCCTGGCGTC TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC 
TCGAAGGATC CCTCGGCCTC CAGCCCCCTT GACAGCACGG CTGCCTCACC CTCCCCAAAG 
AAAGATGCGC TGAGTCTCTC TGGTTTGTCT TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532138.1
CDS	784..7860
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532075.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532075.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAA TCTAATGTTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCTGCGT CAGAAGCCCA GTGCAAACCC TACTACTCTG ACTACTCGCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGACCTCT TCATCACTGG TGTCATCGGG 
CTGAATGTGG TCACGATGGC CATGGAACAT TACCAGCAGC CCCAGATCCT GGACGAGGCT 
CTGAAGATCT GCAACTACAT CTTTACCGTC ATCTTTGTCT TGGAGTCAGT ATTCAAACTT 
GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCTATT 
GTGCTTCTGT CCATCATGGG CATCACGCTG GAAGAGATTG AGGTCAATGC TTCACTGCCC 
ATCAACCCCA CCATCATCCG TATCATGAGG GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG 
TTGAAGATGG CTGTGGGCAT GCGGGCACTG CTGGACACGG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACC TGGGACTTCT CTTCATGCTA TTATTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGACCTGGA GTGTGATGAG ACACACCCTT GTGAGGGCTT GGGCCGGCAT 
GCCACCTTTA GGAACTTTGG TATGGCCTTT CTGACCCTCT TCCGAGTCTC CACTGGTGAC 
AACTGGAATG GTATTATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACCGTCA TCTCACCCAT CTACTTCGTG TCCTTCGTGC TGACGGCCCA GTTTGTGCTG 
GTCAACGTGG TCATAGCCGT GCTGATGAAG CACCTGGAAG AGAGCAACAA AGAGGCCAAG 
GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG CTAGAGATGA AGACACTCAG CCCGCAGCCC 
CACTCCCCGC TGGGCAGCCC CTTCCTCTGG CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC 
AGCCCTAAGC CTGGGGCTCC ACACACCACG GCCCACATTG GAGCAGCCTC TTCAGGCTTC 
TCCCTTGAGC ACCCCACGGA CAGACAGCTG TTTGACACCA TCTCCCTGCT GATCCAGGGC 
TCCCTGGAGG GGGAGCTGAA GCTGATGGAC GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT 
GCCTTCCCTT CCGCCCCCAG CCCGGGCGAC TCCGACCCAC AGATCCCTCT AGCTGAGATG 
GAGGCTCTGT CTCTGACGTC AGAGATTGTG TCTGAACCGT CCTGCTCTCT AGCTCTGACG 
GATGACTCTT TGCCTGATGA CACTCACACA CTCTTACTTA GTGCCCTGGA GAGCAATATG 
GTACCTCACA CTGAGGAGGG GCCAGTCCCC CTAGGACCAG ACCTGCTGAC TGTGAGGAAG 
TCTGGTGTCA GCCGGACACA CTCTCTGCCC AATGACAGCT ACATGTGCCG CAATGGGAGC 
ACTGCCGAGA GATCCCTAGG ACACAGGGGC TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC 
ATCTTGTCTG TTCACTCCCA ACCAGCAGAC ACCAGCTGCA TCCTACAGCT TCCCAAAGAT 
GCACACTATC TGCTCCAGCC TCATGGGGCT CCCACCTGGG GCGCCATCCC TAAACTACCC 
CCACCTGGCC GCTCCCCTCT GGCTCAGAGG CCTCTCAGGC GCCAGGCAGC AATAAGGACT 
GACTCCCTGG ACGTGCAGGG CCTGGGTAGC CGGGAAGACC TGTTGTCAGA GGTGAGTGGG 
CCCTCCTGCC CTCTGACCCG CTCCTCATCC TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG 
CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC AAGCACATCC GCCTGCCAGC CCCTTGCCCA 
GGCCTGGAAC CCAGCTGGGC CAAGGACCCT CAAGAGACCA GAAGCAGCTT AGAGCTGGAC 
ACGGAGCTGA GCTGGATTTC AGGAGACCTC CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC 
CCACGGGACT TGAAAAAATG CTACAGTGTA GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG 
TCCTGGCTAG ACGAACAGAG GAGACACTCC ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC 
CAGCCCCGCC TATGTCCAAG CCCCTCAAGC CTCGGGGGCC AACCTCTTGG GGGCCCTGGG 
AGCCGGCCTA AGAAAAAACT CAGCCCACCC AGTATCTCTA TAGACCCCCC GGAGAGCCAG 
GGCCCTCGGC CCCCATGCAG TCCTGGCGTC TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC 
TCGAAGGATC CCTCGGCCTC CAGCCCCCTT GACAGCACGG CTGCCTCACC CTCCCCAAAG 
AAAGATGCGC TGAGTCTCTC TGGTTTGTCT TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532076.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532139.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X13, mRNA.

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CloneID	OMu45471
Clone ID Related Accession (Same CDS sequence)	XM_006532076.3 , XM_006532076.4
Accession Version	XM_006532076.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532076.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGGACAG ACAGCTGTTT 
GACACCATCT CCCTGCTGAT CCAGGGCTCC CTGGAGGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTTCCG CCCCCAGCCC GGGCGACTCC 
GACCCACAGA TCCCTCTAGC TGAGATGGAG GCTCTGTCTC TGACGTCAGA GATTGTGTCT 
GAACCGTCCT GCTCTCTAGC TCTGACGGAT GACTCTTTGC CTGATGACAC TCACACACTC 
TTACTTAGTG CCCTGGAGAG CAATATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532139.1
CDS	784..7827
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532076.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532076.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGGACAG ACAGCTGTTT 
GACACCATCT CCCTGCTGAT CCAGGGCTCC CTGGAGGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTTCCG CCCCCAGCCC GGGCGACTCC 
GACCCACAGA TCCCTCTAGC TGAGATGGAG GCTCTGTCTC TGACGTCAGA GATTGTGTCT 
GAACCGTCCT GCTCTCTAGC TCTGACGGAT GACTCTTTGC CTGATGACAC TCACACACTC 
TTACTTAGTG CCCTGGAGAG CAATATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532079.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532142.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X16, mRNA.

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CloneID	OMu45474
Clone ID Related Accession (Same CDS sequence)	XM_006532079.3 , XM_006532079.4
Accession Version	XM_006532079.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532079.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAT CTAATGTTGG ACGATGTAAT TGCTTCCGGC 
AGCTCAGCCA GCGCTGCGTC AGAAGCCCAG TGCAAACCCT ACTACTCTGA CTACTCGCGC 
TTCCGGCTCC TCGTCCACCA CCTGTGTACC AGCCACTACC TGGACCTCTT CATCACTGGT 
GTCATCGGGC TGAATGTGGT CACGATGGCC ATGGAACATT ACCAGCAGCC CCAGATCCTG 
GACGAGGCTC TGAAGATCTG CAACTACATC TTTACCGTCA TCTTTGTCTT GGAGTCAGTA 
TTCAAACTTG TGGCCTTCGG CTTCCGCCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCTATTG TGCTTCTGTC CATCATGGGC ATCACGCTGG AAGAGATTGA GGTCAATGCT 
TCACTGCCCA TCAACCCCAC CATCATCCGT ATCATGAGGG TGCTCCGCAT TGCTCGAGTT 
CTGAAGCTGT TGAAGATGGC TGTGGGCATG CGGGCACTGC TGGACACGGT GATGCAGGCC 
CTGCCCCAGG TGGGGAACCT GGGACTTCTC TTCATGCTAT TATTTTTCAT CTTTGCAGCT 
CTGGGCGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CACACCCTTG TGAGGGCTTG 
GGCCGGCATG CCACCTTTAG GAACTTTGGT ATGGCCTTTC TGACCCTCTT CCGAGTCTCC 
ACTGGTGACA ACTGGAATGG TATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACCTGCTACA ACACCGTCAT CTCACCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT CATAGCCGTG CTGATGAAGC ACCTGGAAGA GAGCAACAAA 
GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG GAGCTGGAGC TAGAGATGAA GACACTCAGC 
CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT 
AGCCCTGACA GCCCTAAGCC TGGGGCTCCA CACACCACGG CCCACATTGG AGCAGCCTCT 
TCAGGCTTCT CCCTTGAGCA CCCCACGGAC AGACAGCTGT TTGACACCAT CTCCCTGCTG 
ATCCAGGGCT CCCTGGAGGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTTC CGCCCCCAGC CCGGGCGACT CCGACCCACA GATGGTACCT 
CACACTGAGG AGGGGCCAGT CCCCCTAGGA CCAGACCTGC TGACTGTGAG GAAGTCTGGT 
GTCAGCCGGA CACACTCTCT GCCCAATGAC AGCTACATGT GCCGCAATGG GAGCACTGCC 
GAGAGATCCC TAGGACACAG GGGCTGGGGG CTCCCCAAAG CCCAGTCAGG CTCCATCTTG 
TCTGTTCACT CCCAACCAGC AGACACCAGC TGCATCCTAC AGCTTCCCAA AGATGCACAC 
TATCTGCTCC AGCCTCATGG GGCTCCCACC TGGGGCGCCA TCCCTAAACT ACCCCCACCT 
GGCCGCTCCC CTCTGGCTCA GAGGCCTCTC AGGCGCCAGG CAGCAATAAG GACTGACTCC 
CTGGACGTGC AGGGCCTGGG TAGCCGGGAA GACCTGTTGT CAGAGGTGAG TGGGCCCTCC 
TGCCCTCTGA CCCGCTCCTC ATCCTTCTGG GGCGGGTCGA GCATCCAGGT GCAGCAGCGC 
TCCGGCAGCC AGAGCAAAGT CTCCAAGCAC ATCCGCCTGC CAGCCCCTTG CCCAGGCCTG 
GAACCCAGCT GGGCCAAGGA CCCTCAAGAG ACCAGAAGCA GCTTAGAGCT GGACACGGAG 
CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC AGCAGTCAGG AAGAACCCCT GTCCCCACGG 
GACTTGAAAA AATGCTACAG TGTAGAGGCC CAGAGCTGCC GGCGCAGGCC TGGGTCCTGG 
CTAGACGAAC AGAGGAGACA CTCCATCGCT GTCAGCTGCC TGGACAGCGG CTCCCAGCCC 
CGCCTATGTC CAAGCCCCTC AAGCCTCGGG GGCCAACCTC TTGGGGGCCC TGGGAGCCGG 
CCTAAGAAAA AACTCAGCCC ACCCAGTATC TCTATAGACC CCCCGGAGAG CCAGGGCCCT 
CGGCCCCCAT GCAGTCCTGG CGTCTGCCTC AGGAGGAGGG CGCCGGCCAG TGACTCGAAG 
GATCCCTCGG CCTCCAGCCC CCTTGACAGC ACGGCTGCCT CACCCTCCCC AAAGAAAGAT 
GCGCTGAGTC TCTCTGGTTT GTCTTCTGAC CCAACAGACC TGGATCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532142.1
CDS	784..7794
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532079.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532079.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAT CTAATGTTGG ACGATGTAAT TGCTTCCGGC 
AGCTCAGCCA GCGCTGCGTC AGAAGCCCAG TGCAAACCCT ACTACTCTGA CTACTCGCGC 
TTCCGGCTCC TCGTCCACCA CCTGTGTACC AGCCACTACC TGGACCTCTT CATCACTGGT 
GTCATCGGGC TGAATGTGGT CACGATGGCC ATGGAACATT ACCAGCAGCC CCAGATCCTG 
GACGAGGCTC TGAAGATCTG CAACTACATC TTTACCGTCA TCTTTGTCTT GGAGTCAGTA 
TTCAAACTTG TGGCCTTCGG CTTCCGCCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCTATTG TGCTTCTGTC CATCATGGGC ATCACGCTGG AAGAGATTGA GGTCAATGCT 
TCACTGCCCA TCAACCCCAC CATCATCCGT ATCATGAGGG TGCTCCGCAT TGCTCGAGTT 
CTGAAGCTGT TGAAGATGGC TGTGGGCATG CGGGCACTGC TGGACACGGT GATGCAGGCC 
CTGCCCCAGG TGGGGAACCT GGGACTTCTC TTCATGCTAT TATTTTTCAT CTTTGCAGCT 
CTGGGCGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CACACCCTTG TGAGGGCTTG 
GGCCGGCATG CCACCTTTAG GAACTTTGGT ATGGCCTTTC TGACCCTCTT CCGAGTCTCC 
ACTGGTGACA ACTGGAATGG TATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACCTGCTACA ACACCGTCAT CTCACCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT CATAGCCGTG CTGATGAAGC ACCTGGAAGA GAGCAACAAA 
GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG GAGCTGGAGC TAGAGATGAA GACACTCAGC 
CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT 
AGCCCTGACA GCCCTAAGCC TGGGGCTCCA CACACCACGG CCCACATTGG AGCAGCCTCT 
TCAGGCTTCT CCCTTGAGCA CCCCACGGAC AGACAGCTGT TTGACACCAT CTCCCTGCTG 
ATCCAGGGCT CCCTGGAGGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTTC CGCCCCCAGC CCGGGCGACT CCGACCCACA GATGGTACCT 
CACACTGAGG AGGGGCCAGT CCCCCTAGGA CCAGACCTGC TGACTGTGAG GAAGTCTGGT 
GTCAGCCGGA CACACTCTCT GCCCAATGAC AGCTACATGT GCCGCAATGG GAGCACTGCC 
GAGAGATCCC TAGGACACAG GGGCTGGGGG CTCCCCAAAG CCCAGTCAGG CTCCATCTTG 
TCTGTTCACT CCCAACCAGC AGACACCAGC TGCATCCTAC AGCTTCCCAA AGATGCACAC 
TATCTGCTCC AGCCTCATGG GGCTCCCACC TGGGGCGCCA TCCCTAAACT ACCCCCACCT 
GGCCGCTCCC CTCTGGCTCA GAGGCCTCTC AGGCGCCAGG CAGCAATAAG GACTGACTCC 
CTGGACGTGC AGGGCCTGGG TAGCCGGGAA GACCTGTTGT CAGAGGTGAG TGGGCCCTCC 
TGCCCTCTGA CCCGCTCCTC ATCCTTCTGG GGCGGGTCGA GCATCCAGGT GCAGCAGCGC 
TCCGGCAGCC AGAGCAAAGT CTCCAAGCAC ATCCGCCTGC CAGCCCCTTG CCCAGGCCTG 
GAACCCAGCT GGGCCAAGGA CCCTCAAGAG ACCAGAAGCA GCTTAGAGCT GGACACGGAG 
CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC AGCAGTCAGG AAGAACCCCT GTCCCCACGG 
GACTTGAAAA AATGCTACAG TGTAGAGGCC CAGAGCTGCC GGCGCAGGCC TGGGTCCTGG 
CTAGACGAAC AGAGGAGACA CTCCATCGCT GTCAGCTGCC TGGACAGCGG CTCCCAGCCC 
CGCCTATGTC CAAGCCCCTC AAGCCTCGGG GGCCAACCTC TTGGGGGCCC TGGGAGCCGG 
CCTAAGAAAA AACTCAGCCC ACCCAGTATC TCTATAGACC CCCCGGAGAG CCAGGGCCCT 
CGGCCCCCAT GCAGTCCTGG CGTCTGCCTC AGGAGGAGGG CGCCGGCCAG TGACTCGAAG 
GATCCCTCGG CCTCCAGCCC CCTTGACAGC ACGGCTGCCT CACCCTCCCC AAAGAAAGAT 
GCGCTGAGTC TCTCTGGTTT GTCTTCTGAC CCAACAGACC TGGATCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532080.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532143.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X19, mRNA.

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CloneID	OMu45475
Clone ID Related Accession (Same CDS sequence)	XM_006532080.4 , XM_006532080.3
Accession Version	XM_006532080.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532080.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA AGCCCAGTGC 
AAACCCTACT ACTCTGACTA CTCGCGCTTC CGGCTCCTCG TCCACCACCT GTGTACCAGC 
CACTACCTGG ACCTCTTCAT CACTGGTGTC ATCGGGCTGA ATGTGGTCAC GATGGCCATG 
GAACATTACC AGCAGCCCCA GATCCTGGAC GAGGCTCTGA AGATCTGCAA CTACATCTTT 
ACCGTCATCT TTGTCTTGGA GTCAGTATTC AAACTTGTGG CCTTCGGCTT CCGCCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCTATTGTGC TTCTGTCCAT CATGGGCATC 
ACGCTGGAAG AGATTGAGGT CAATGCTTCA CTGCCCATCA ACCCCACCAT CATCCGTATC 
ATGAGGGTGC TCCGCATTGC TCGAGTTCTG AAGCTGTTGA AGATGGCTGT GGGCATGCGG 
GCACTGCTGG ACACGGTGAT GCAGGCCCTG CCCCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGCTATTAT TTTTCATCTT TGCAGCTCTG GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACAC ACCCTTGTGA GGGCTTGGGC CGGCATGCCA CCTTTAGGAA CTTTGGTATG 
GCCTTTCTGA CCCTCTTCCG AGTCTCCACT GGTGACAACT GGAATGGTAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACC TGCTACAACA CCGTCATCTC ACCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG 
ATGAAGCACC TGGAAGAGAG CAACAAAGAG GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG 
CTGGAGCTAG AGATGAAGAC ACTCAGCCCG CAGCCCCACT CCCCGCTGGG CAGCCCCTTC 
CTCTGGCCTG GGGTGGAAGG TGTCAATAGC CCTGACAGCC CTAAGCCTGG GGCTCCACAC 
ACCACGGCCC ACATTGGAGC AGCCTCTTCA GGCTTCTCCC TTGAGCACCC CACGGACAGA 
CAGCTGTTTG ACACCATCTC CCTGCTGATC CAGGGCTCCC TGGAGGGGGA GCTGAAGCTG 
ATGGACGAGC TGGCAGGCCC AGGGGGCCAG CCCTCTGCCT TCCCTTCCGC CCCCAGCCCG 
GGCGACTCCG ACCCACAGAT GGTACCTCAC ACTGAGGAGG GGCCAGTCCC CCTAGGACCA 
GACCTGCTGA CTGTGAGGAA GTCTGGTGTC AGCCGGACAC ACTCTCTGCC CAATGACAGC 
TACATGTGCC GCAATGGGAG CACTGCCGAG AGATCCCTAG GACACAGGGG CTGGGGGCTC 
CCCAAAGCCC AGTCAGGCTC CATCTTGTCT GTTCACTCCC AACCAGCAGA CACCAGCTGC 
ATCCTACAGC TTCCCAAAGA TGCACACTAT CTGCTCCAGC CTCATGGGGC TCCCACCTGG 
GGCGCCATCC CTAAACTACC CCCACCTGGC CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG 
CGCCAGGCAG CAATAAGGAC TGACTCCCTG GACGTGCAGG GCCTGGGTAG CCGGGAAGAC 
CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC CCTCTGACCC GCTCCTCATC CTTCTGGGGC 
GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC GGCAGCCAGA GCAAAGTCTC CAAGCACATC 
CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA CCCAGCTGGG CCAAGGACCC TCAAGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGCCCAGC 
AGTCAGGAAG AACCCCTGTC CCCACGGGAC TTGAAAAAAT GCTACAGTGT AGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA GACGAACAGA GGAGACACTC CATCGCTGTC 
AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC CTATGTCCAA GCCCCTCAAG CCTCGGGGGC 
CAACCTCTTG GGGGCCCTGG GAGCCGGCCT AAGAAAAAAC TCAGCCCACC CAGTATCTCT 
ATAGACCCCC CGGAGAGCCA GGGCCCTCGG CCCCCATGCA GTCCTGGCGT CTGCCTCAGG 
AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT CCCTCGGCCT CCAGCCCCCT TGACAGCACG 
GCTGCCTCAC CCTCCCCAAA GAAAGATGCG CTGAGTCTCT CTGGTTTGTC TTCTGACCCA 
ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532143.1
CDS	784..7761
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532080.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532080.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA AGCCCAGTGC 
AAACCCTACT ACTCTGACTA CTCGCGCTTC CGGCTCCTCG TCCACCACCT GTGTACCAGC 
CACTACCTGG ACCTCTTCAT CACTGGTGTC ATCGGGCTGA ATGTGGTCAC GATGGCCATG 
GAACATTACC AGCAGCCCCA GATCCTGGAC GAGGCTCTGA AGATCTGCAA CTACATCTTT 
ACCGTCATCT TTGTCTTGGA GTCAGTATTC AAACTTGTGG CCTTCGGCTT CCGCCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCTATTGTGC TTCTGTCCAT CATGGGCATC 
ACGCTGGAAG AGATTGAGGT CAATGCTTCA CTGCCCATCA ACCCCACCAT CATCCGTATC 
ATGAGGGTGC TCCGCATTGC TCGAGTTCTG AAGCTGTTGA AGATGGCTGT GGGCATGCGG 
GCACTGCTGG ACACGGTGAT GCAGGCCCTG CCCCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGCTATTAT TTTTCATCTT TGCAGCTCTG GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACAC ACCCTTGTGA GGGCTTGGGC CGGCATGCCA CCTTTAGGAA CTTTGGTATG 
GCCTTTCTGA CCCTCTTCCG AGTCTCCACT GGTGACAACT GGAATGGTAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACC TGCTACAACA CCGTCATCTC ACCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG 
ATGAAGCACC TGGAAGAGAG CAACAAAGAG GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG 
CTGGAGCTAG AGATGAAGAC ACTCAGCCCG CAGCCCCACT CCCCGCTGGG CAGCCCCTTC 
CTCTGGCCTG GGGTGGAAGG TGTCAATAGC CCTGACAGCC CTAAGCCTGG GGCTCCACAC 
ACCACGGCCC ACATTGGAGC AGCCTCTTCA GGCTTCTCCC TTGAGCACCC CACGGACAGA 
CAGCTGTTTG ACACCATCTC CCTGCTGATC CAGGGCTCCC TGGAGGGGGA GCTGAAGCTG 
ATGGACGAGC TGGCAGGCCC AGGGGGCCAG CCCTCTGCCT TCCCTTCCGC CCCCAGCCCG 
GGCGACTCCG ACCCACAGAT GGTACCTCAC ACTGAGGAGG GGCCAGTCCC CCTAGGACCA 
GACCTGCTGA CTGTGAGGAA GTCTGGTGTC AGCCGGACAC ACTCTCTGCC CAATGACAGC 
TACATGTGCC GCAATGGGAG CACTGCCGAG AGATCCCTAG GACACAGGGG CTGGGGGCTC 
CCCAAAGCCC AGTCAGGCTC CATCTTGTCT GTTCACTCCC AACCAGCAGA CACCAGCTGC 
ATCCTACAGC TTCCCAAAGA TGCACACTAT CTGCTCCAGC CTCATGGGGC TCCCACCTGG 
GGCGCCATCC CTAAACTACC CCCACCTGGC CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG 
CGCCAGGCAG CAATAAGGAC TGACTCCCTG GACGTGCAGG GCCTGGGTAG CCGGGAAGAC 
CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC CCTCTGACCC GCTCCTCATC CTTCTGGGGC 
GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC GGCAGCCAGA GCAAAGTCTC CAAGCACATC 
CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA CCCAGCTGGG CCAAGGACCC TCAAGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGCCCAGC 
AGTCAGGAAG AACCCCTGTC CCCACGGGAC TTGAAAAAAT GCTACAGTGT AGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA GACGAACAGA GGAGACACTC CATCGCTGTC 
AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC CTATGTCCAA GCCCCTCAAG CCTCGGGGGC 
CAACCTCTTG GGGGCCCTGG GAGCCGGCCT AAGAAAAAAC TCAGCCCACC CAGTATCTCT 
ATAGACCCCC CGGAGAGCCA GGGCCCTCGG CCCCCATGCA GTCCTGGCGT CTGCCTCAGG 
AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT CCCTCGGCCT CCAGCCCCCT TGACAGCACG 
GCTGCCTCAC CCTCCCCAAA GAAAGATGCG CTGAGTCTCT CTGGTTTGTC TTCTGACCCA 
ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532081.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532144.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X20, mRNA.

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CloneID	OMu45476
Clone ID Related Accession (Same CDS sequence)	XM_006532081.3 , XM_006532081.4
Accession Version	XM_006532081.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532081.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAA GCCCAGTGCA AACCCTACTA CTCTGACTAC 
TCGCGCTTCC GGCTCCTCGT CCACCACCTG TGTACCAGCC ACTACCTGGA CCTCTTCATC 
ACTGGTGTCA TCGGGCTGAA TGTGGTCACG ATGGCCATGG AACATTACCA GCAGCCCCAG 
ATCCTGGACG AGGCTCTGAA GATCTGCAAC TACATCTTTA CCGTCATCTT TGTCTTGGAG 
TCAGTATTCA AACTTGTGGC CTTCGGCTTC CGCCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CTATTGTGCT TCTGTCCATC ATGGGCATCA CGCTGGAAGA GATTGAGGTC 
AATGCTTCAC TGCCCATCAA CCCCACCATC ATCCGTATCA TGAGGGTGCT CCGCATTGCT 
CGAGTTCTGA AGCTGTTGAA GATGGCTGTG GGCATGCGGG CACTGCTGGA CACGGTGATG 
CAGGCCCTGC CCCAGGTGGG GAACCTGGGA CTTCTCTTCA TGCTATTATT TTTCATCTTT 
GCAGCTCTGG GCGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACACA CCCTTGTGAG 
GGCTTGGGCC GGCATGCCAC CTTTAGGAAC TTTGGTATGG CCTTTCTGAC CCTCTTCCGA 
GTCTCCACTG GTGACAACTG GAATGGTATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACCT GCTACAACAC CGTCATCTCA CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTCATA GCCGTGCTGA TGAAGCACCT GGAAGAGAGC 
AACAAAGAGG CCAAGGAGGA GGCGGAGTTG GAGGCGGAGC TGGAGCTAGA GATGAAGACA 
CTCAGCCCGC AGCCCCACTC CCCGCTGGGC AGCCCCTTCC TCTGGCCTGG GGTGGAAGGT 
GTCAATAGCC CTGACAGCCC TAAGCCTGGG GCTCCACACA CCACGGCCCA CATTGGAGCA 
GCCTCTTCAG GCTTCTCCCT TGAGCACCCC ACGGACAGAC AGCTGTTTGA CACCATCTCC 
CTGCTGATCC AGGGCTCCCT GGAGGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA 
GGGGGCCAGC CCTCTGCCTT CCCTTCCGCC CCCAGCCCGG GCGACTCCGA CCCACAGATG 
GTACCTCACA CTGAGGAGGG GCCAGTCCCC CTAGGACCAG ACCTGCTGAC TGTGAGGAAG 
TCTGGTGTCA GCCGGACACA CTCTCTGCCC AATGACAGCT ACATGTGCCG CAATGGGAGC 
ACTGCCGAGA GATCCCTAGG ACACAGGGGC TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC 
ATCTTGTCTG TTCACTCCCA ACCAGCAGAC ACCAGCTGCA TCCTACAGCT TCCCAAAGAT 
GCACACTATC TGCTCCAGCC TCATGGGGCT CCCACCTGGG GCGCCATCCC TAAACTACCC 
CCACCTGGCC GCTCCCCTCT GGCTCAGAGG CCTCTCAGGC GCCAGGCAGC AATAAGGACT 
GACTCCCTGG ACGTGCAGGG CCTGGGTAGC CGGGAAGACC TGTTGTCAGA GGTGAGTGGG 
CCCTCCTGCC CTCTGACCCG CTCCTCATCC TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG 
CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC AAGCACATCC GCCTGCCAGC CCCTTGCCCA 
GGCCTGGAAC CCAGCTGGGC CAAGGACCCT CAAGAGACCA GAAGCAGCTT AGAGCTGGAC 
ACGGAGCTGA GCTGGATTTC AGGAGACCTC CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC 
CCACGGGACT TGAAAAAATG CTACAGTGTA GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG 
TCCTGGCTAG ACGAACAGAG GAGACACTCC ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC 
CAGCCCCGCC TATGTCCAAG CCCCTCAAGC CTCGGGGGCC AACCTCTTGG GGGCCCTGGG 
AGCCGGCCTA AGAAAAAACT CAGCCCACCC AGTATCTCTA TAGACCCCCC GGAGAGCCAG 
GGCCCTCGGC CCCCATGCAG TCCTGGCGTC TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC 
TCGAAGGATC CCTCGGCCTC CAGCCCCCTT GACAGCACGG CTGCCTCACC CTCCCCAAAG 
AAAGATGCGC TGAGTCTCTC TGGTTTGTCT TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532144.1
CDS	784..7740
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532081.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532081.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAA GCCCAGTGCA AACCCTACTA CTCTGACTAC 
TCGCGCTTCC GGCTCCTCGT CCACCACCTG TGTACCAGCC ACTACCTGGA CCTCTTCATC 
ACTGGTGTCA TCGGGCTGAA TGTGGTCACG ATGGCCATGG AACATTACCA GCAGCCCCAG 
ATCCTGGACG AGGCTCTGAA GATCTGCAAC TACATCTTTA CCGTCATCTT TGTCTTGGAG 
TCAGTATTCA AACTTGTGGC CTTCGGCTTC CGCCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CTATTGTGCT TCTGTCCATC ATGGGCATCA CGCTGGAAGA GATTGAGGTC 
AATGCTTCAC TGCCCATCAA CCCCACCATC ATCCGTATCA TGAGGGTGCT CCGCATTGCT 
CGAGTTCTGA AGCTGTTGAA GATGGCTGTG GGCATGCGGG CACTGCTGGA CACGGTGATG 
CAGGCCCTGC CCCAGGTGGG GAACCTGGGA CTTCTCTTCA TGCTATTATT TTTCATCTTT 
GCAGCTCTGG GCGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACACA CCCTTGTGAG 
GGCTTGGGCC GGCATGCCAC CTTTAGGAAC TTTGGTATGG CCTTTCTGAC CCTCTTCCGA 
GTCTCCACTG GTGACAACTG GAATGGTATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACCT GCTACAACAC CGTCATCTCA CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTCATA GCCGTGCTGA TGAAGCACCT GGAAGAGAGC 
AACAAAGAGG CCAAGGAGGA GGCGGAGTTG GAGGCGGAGC TGGAGCTAGA GATGAAGACA 
CTCAGCCCGC AGCCCCACTC CCCGCTGGGC AGCCCCTTCC TCTGGCCTGG GGTGGAAGGT 
GTCAATAGCC CTGACAGCCC TAAGCCTGGG GCTCCACACA CCACGGCCCA CATTGGAGCA 
GCCTCTTCAG GCTTCTCCCT TGAGCACCCC ACGGACAGAC AGCTGTTTGA CACCATCTCC 
CTGCTGATCC AGGGCTCCCT GGAGGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA 
GGGGGCCAGC CCTCTGCCTT CCCTTCCGCC CCCAGCCCGG GCGACTCCGA CCCACAGATG 
GTACCTCACA CTGAGGAGGG GCCAGTCCCC CTAGGACCAG ACCTGCTGAC TGTGAGGAAG 
TCTGGTGTCA GCCGGACACA CTCTCTGCCC AATGACAGCT ACATGTGCCG CAATGGGAGC 
ACTGCCGAGA GATCCCTAGG ACACAGGGGC TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC 
ATCTTGTCTG TTCACTCCCA ACCAGCAGAC ACCAGCTGCA TCCTACAGCT TCCCAAAGAT 
GCACACTATC TGCTCCAGCC TCATGGGGCT CCCACCTGGG GCGCCATCCC TAAACTACCC 
CCACCTGGCC GCTCCCCTCT GGCTCAGAGG CCTCTCAGGC GCCAGGCAGC AATAAGGACT 
GACTCCCTGG ACGTGCAGGG CCTGGGTAGC CGGGAAGACC TGTTGTCAGA GGTGAGTGGG 
CCCTCCTGCC CTCTGACCCG CTCCTCATCC TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG 
CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC AAGCACATCC GCCTGCCAGC CCCTTGCCCA 
GGCCTGGAAC CCAGCTGGGC CAAGGACCCT CAAGAGACCA GAAGCAGCTT AGAGCTGGAC 
ACGGAGCTGA GCTGGATTTC AGGAGACCTC CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC 
CCACGGGACT TGAAAAAATG CTACAGTGTA GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG 
TCCTGGCTAG ACGAACAGAG GAGACACTCC ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC 
CAGCCCCGCC TATGTCCAAG CCCCTCAAGC CTCGGGGGCC AACCTCTTGG GGGCCCTGGG 
AGCCGGCCTA AGAAAAAACT CAGCCCACCC AGTATCTCTA TAGACCCCCC GGAGAGCCAG 
GGCCCTCGGC CCCCATGCAG TCCTGGCGTC TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC 
TCGAAGGATC CCTCGGCCTC CAGCCCCCTT GACAGCACGG CTGCCTCACC CTCCCCAAAG 
AAAGATGCGC TGAGTCTCTC TGGTTTGTCT TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532082.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532145.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X21, mRNA.

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CloneID	OMu45477
Clone ID Related Accession (Same CDS sequence)	XM_006532082.3 , XM_006532082.4
Accession Version	XM_006532082.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532082.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAA TCTAATGTTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCTGCGT CAGAAGCCCA GTGCAAACCC TACTACTCTG ACTACTCGCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGACCTCT TCATCACTGG TGTCATCGGG 
CTGAATGTGG TCACGATGGC CATGGAACAT TACCAGCAGC CCCAGATCCT GGACGAGGCT 
CTGAAGATCT GCAACTACAT CTTTACCGTC ATCTTTGTCT TGGAGTCAGT ATTCAAACTT 
GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCTATT 
GTGCTTCTGT CCATCATGGG CATCACGCTG GAAGAGATTG AGGTCAATGC TTCACTGCCC 
ATCAACCCCA CCATCATCCG TATCATGAGG GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG 
TTGAAGATGG CTGTGGGCAT GCGGGCACTG CTGGACACGG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACC TGGGACTTCT CTTCATGCTA TTATTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGACCTGGA GTGTGATGAG ACACACCCTT GTGAGGGCTT GGGCCGGCAT 
GCCACCTTTA GGAACTTTGG TATGGCCTTT CTGACCCTCT TCCGAGTCTC CACTGGTGAC 
AACTGGAATG GTATTATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACCGTCA TCTCACCCAT CTACTTCGTG TCCTTCGTGC TGACGGCCCA GTTTGTGCTG 
GTCAACGTGG TCATAGCCGT GCTGATGAAG CACCTGGAAG AGAGCAACAA AGAGGCCAAG 
GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG CTAGAGATGA AGACACTCAG CCCGCAGCCC 
CACTCCCCGC TGGGCAGCCC CTTCCTCTGG CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC 
AGCCCTAAGC CTGGGGCTCC ACACACCACG GCCCACATTG GAGCAGCCTC TTCAGGCTTC 
TCCCTTGAGC ACCCCACGAT CCCTCTAGCT GAGATGGAGG CTCTGTCTCT GACGTCAGAG 
ATTGTGTCTG AACCGTCCTG CTCTCTAGCT CTGACGGATG ACTCTTTGCC TGATGACACT 
CACACACTCT TACTTAGTGC CCTGGAGAGC AATATGGTAC CTCACACTGA GGAGGGGCCA 
GTCCCCCTAG GACCAGACCT GCTGACTGTG AGGAAGTCTG GTGTCAGCCG GACACACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGCAAT GGGAGCACTG CCGAGAGATC CCTAGGACAC 
AGGGGCTGGG GGCTCCCCAA AGCCCAGTCA GGCTCCATCT TGTCTGTTCA CTCCCAACCA 
GCAGACACCA GCTGCATCCT ACAGCTTCCC AAAGATGCAC ACTATCTGCT CCAGCCTCAT 
GGGGCTCCCA CCTGGGGCGC CATCCCTAAA CTACCCCCAC CTGGCCGCTC CCCTCTGGCT 
CAGAGGCCTC TCAGGCGCCA GGCAGCAATA AGGACTGACT CCCTGGACGT GCAGGGCCTG 
GGTAGCCGGG AAGACCTGTT GTCAGAGGTG AGTGGGCCCT CCTGCCCTCT GACCCGCTCC 
TCATCCTTCT GGGGCGGGTC GAGCATCCAG GTGCAGCAGC GCTCCGGCAG CCAGAGCAAA 
GTCTCCAAGC ACATCCGCCT GCCAGCCCCT TGCCCAGGCC TGGAACCCAG CTGGGCCAAG 
GACCCTCAAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCAGCAGTCA GGAAGAACCC CTGTCCCCAC GGGACTTGAA AAAATGCTAC 
AGTGTAGAGG CCCAGAGCTG CCGGCGCAGG CCTGGGTCCT GGCTAGACGA ACAGAGGAGA 
CACTCCATCG CTGTCAGCTG CCTGGACAGC GGCTCCCAGC CCCGCCTATG TCCAAGCCCC 
TCAAGCCTCG GGGGCCAACC TCTTGGGGGC CCTGGGAGCC GGCCTAAGAA AAAACTCAGC 
CCACCCAGTA TCTCTATAGA CCCCCCGGAG AGCCAGGGCC CTCGGCCCCC ATGCAGTCCT 
GGCGTCTGCC TCAGGAGGAG GGCGCCGGCC AGTGACTCGA AGGATCCCTC GGCCTCCAGC 
CCCCTTGACA GCACGGCTGC CTCACCCTCC CCAAAGAAAG ATGCGCTGAG TCTCTCTGGT 
TTGTCTTCTG ACCCAACAGA CCTGGATCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532145.1
CDS	784..7716
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532082.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532082.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAA TCTAATGTTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCTGCGT CAGAAGCCCA GTGCAAACCC TACTACTCTG ACTACTCGCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGACCTCT TCATCACTGG TGTCATCGGG 
CTGAATGTGG TCACGATGGC CATGGAACAT TACCAGCAGC CCCAGATCCT GGACGAGGCT 
CTGAAGATCT GCAACTACAT CTTTACCGTC ATCTTTGTCT TGGAGTCAGT ATTCAAACTT 
GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCTATT 
GTGCTTCTGT CCATCATGGG CATCACGCTG GAAGAGATTG AGGTCAATGC TTCACTGCCC 
ATCAACCCCA CCATCATCCG TATCATGAGG GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG 
TTGAAGATGG CTGTGGGCAT GCGGGCACTG CTGGACACGG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACC TGGGACTTCT CTTCATGCTA TTATTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGACCTGGA GTGTGATGAG ACACACCCTT GTGAGGGCTT GGGCCGGCAT 
GCCACCTTTA GGAACTTTGG TATGGCCTTT CTGACCCTCT TCCGAGTCTC CACTGGTGAC 
AACTGGAATG GTATTATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACCGTCA TCTCACCCAT CTACTTCGTG TCCTTCGTGC TGACGGCCCA GTTTGTGCTG 
GTCAACGTGG TCATAGCCGT GCTGATGAAG CACCTGGAAG AGAGCAACAA AGAGGCCAAG 
GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG CTAGAGATGA AGACACTCAG CCCGCAGCCC 
CACTCCCCGC TGGGCAGCCC CTTCCTCTGG CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC 
AGCCCTAAGC CTGGGGCTCC ACACACCACG GCCCACATTG GAGCAGCCTC TTCAGGCTTC 
TCCCTTGAGC ACCCCACGAT CCCTCTAGCT GAGATGGAGG CTCTGTCTCT GACGTCAGAG 
ATTGTGTCTG AACCGTCCTG CTCTCTAGCT CTGACGGATG ACTCTTTGCC TGATGACACT 
CACACACTCT TACTTAGTGC CCTGGAGAGC AATATGGTAC CTCACACTGA GGAGGGGCCA 
GTCCCCCTAG GACCAGACCT GCTGACTGTG AGGAAGTCTG GTGTCAGCCG GACACACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGCAAT GGGAGCACTG CCGAGAGATC CCTAGGACAC 
AGGGGCTGGG GGCTCCCCAA AGCCCAGTCA GGCTCCATCT TGTCTGTTCA CTCCCAACCA 
GCAGACACCA GCTGCATCCT ACAGCTTCCC AAAGATGCAC ACTATCTGCT CCAGCCTCAT 
GGGGCTCCCA CCTGGGGCGC CATCCCTAAA CTACCCCCAC CTGGCCGCTC CCCTCTGGCT 
CAGAGGCCTC TCAGGCGCCA GGCAGCAATA AGGACTGACT CCCTGGACGT GCAGGGCCTG 
GGTAGCCGGG AAGACCTGTT GTCAGAGGTG AGTGGGCCCT CCTGCCCTCT GACCCGCTCC 
TCATCCTTCT GGGGCGGGTC GAGCATCCAG GTGCAGCAGC GCTCCGGCAG CCAGAGCAAA 
GTCTCCAAGC ACATCCGCCT GCCAGCCCCT TGCCCAGGCC TGGAACCCAG CTGGGCCAAG 
GACCCTCAAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCAGCAGTCA GGAAGAACCC CTGTCCCCAC GGGACTTGAA AAAATGCTAC 
AGTGTAGAGG CCCAGAGCTG CCGGCGCAGG CCTGGGTCCT GGCTAGACGA ACAGAGGAGA 
CACTCCATCG CTGTCAGCTG CCTGGACAGC GGCTCCCAGC CCCGCCTATG TCCAAGCCCC 
TCAAGCCTCG GGGGCCAACC TCTTGGGGGC CCTGGGAGCC GGCCTAAGAA AAAACTCAGC 
CCACCCAGTA TCTCTATAGA CCCCCCGGAG AGCCAGGGCC CTCGGCCCCC ATGCAGTCCT 
GGCGTCTGCC TCAGGAGGAG GGCGCCGGCC AGTGACTCGA AGGATCCCTC GGCCTCCAGC 
CCCCTTGACA GCACGGCTGC CTCACCCTCC CCAAAGAAAG ATGCGCTGAG TCTCTCTGGT 
TTGTCTTCTG ACCCAACAGA CCTGGATCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532083.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532146.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X22, mRNA.

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CloneID	OMu45478
Clone ID Related Accession (Same CDS sequence)	XM_006532083.3 , XM_006532083.4
Accession Version	XM_006532083.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532083.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGGACAG ACAGCTGTTT 
GACACCATCT CCCTGCTGAT CCAGGGCTCC CTGGAGGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTTCCG CCCCCAGCCC GGGCGACTCC 
GACCCACAGA TGGTACCTCA CACTGAGGAG GGGCCAGTCC CCCTAGGACC AGACCTGCTG 
ACTGTGAGGA AGTCTGGTGT CAGCCGGACA CACTCTCTGC CCAATGACAG CTACATGTGC 
CGCAATGGGA GCACTGCCGA GAGATCCCTA GGACACAGGG GCTGGGGGCT CCCCAAAGCC 
CAGTCAGGCT CCATCTTGTC TGTTCACTCC CAACCAGCAG ACACCAGCTG CATCCTACAG 
CTTCCCAAAG ATGCACACTA TCTGCTCCAG CCTCATGGGG CTCCCACCTG GGGCGCCATC 
CCTAAACTAC CCCCACCTGG CCGCTCCCCT CTGGCTCAGA GGCCTCTCAG GCGCCAGGCA 
GCAATAAGGA CTGACTCCCT GGACGTGCAG GGCCTGGGTA GCCGGGAAGA CCTGTTGTCA 
GAGGTGAGTG GGCCCTCCTG CCCTCTGACC CGCTCCTCAT CCTTCTGGGG CGGGTCGAGC 
ATCCAGGTGC AGCAGCGCTC CGGCAGCCAG AGCAAAGTCT CCAAGCACAT CCGCCTGCCA 
GCCCCTTGCC CAGGCCTGGA ACCCAGCTGG GCCAAGGACC CTCAAGAGAC CAGAAGCAGC 
TTAGAGCTGG ACACGGAGCT GAGCTGGATT TCAGGAGACC TCCTGCCCAG CAGTCAGGAA 
GAACCCCTGT CCCCACGGGA CTTGAAAAAA TGCTACAGTG TAGAGGCCCA GAGCTGCCGG 
CGCAGGCCTG GGTCCTGGCT AGACGAACAG AGGAGACACT CCATCGCTGT CAGCTGCCTG 
GACAGCGGCT CCCAGCCCCG CCTATGTCCA AGCCCCTCAA GCCTCGGGGG CCAACCTCTT 
GGGGGCCCTG GGAGCCGGCC TAAGAAAAAA CTCAGCCCAC CCAGTATCTC TATAGACCCC 
CCGGAGAGCC AGGGCCCTCG GCCCCCATGC AGTCCTGGCG TCTGCCTCAG GAGGAGGGCG 
CCGGCCAGTG ACTCGAAGGA TCCCTCGGCC TCCAGCCCCC TTGACAGCAC GGCTGCCTCA 
CCCTCCCCAA AGAAAGATGC GCTGAGTCTC TCTGGTTTGT CTTCTGACCC AACAGACCTG 
GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532146.1
CDS	784..7692
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532083.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532083.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGGACAG ACAGCTGTTT 
GACACCATCT CCCTGCTGAT CCAGGGCTCC CTGGAGGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTTCCG CCCCCAGCCC GGGCGACTCC 
GACCCACAGA TGGTACCTCA CACTGAGGAG GGGCCAGTCC CCCTAGGACC AGACCTGCTG 
ACTGTGAGGA AGTCTGGTGT CAGCCGGACA CACTCTCTGC CCAATGACAG CTACATGTGC 
CGCAATGGGA GCACTGCCGA GAGATCCCTA GGACACAGGG GCTGGGGGCT CCCCAAAGCC 
CAGTCAGGCT CCATCTTGTC TGTTCACTCC CAACCAGCAG ACACCAGCTG CATCCTACAG 
CTTCCCAAAG ATGCACACTA TCTGCTCCAG CCTCATGGGG CTCCCACCTG GGGCGCCATC 
CCTAAACTAC CCCCACCTGG CCGCTCCCCT CTGGCTCAGA GGCCTCTCAG GCGCCAGGCA 
GCAATAAGGA CTGACTCCCT GGACGTGCAG GGCCTGGGTA GCCGGGAAGA CCTGTTGTCA 
GAGGTGAGTG GGCCCTCCTG CCCTCTGACC CGCTCCTCAT CCTTCTGGGG CGGGTCGAGC 
ATCCAGGTGC AGCAGCGCTC CGGCAGCCAG AGCAAAGTCT CCAAGCACAT CCGCCTGCCA 
GCCCCTTGCC CAGGCCTGGA ACCCAGCTGG GCCAAGGACC CTCAAGAGAC CAGAAGCAGC 
TTAGAGCTGG ACACGGAGCT GAGCTGGATT TCAGGAGACC TCCTGCCCAG CAGTCAGGAA 
GAACCCCTGT CCCCACGGGA CTTGAAAAAA TGCTACAGTG TAGAGGCCCA GAGCTGCCGG 
CGCAGGCCTG GGTCCTGGCT AGACGAACAG AGGAGACACT CCATCGCTGT CAGCTGCCTG 
GACAGCGGCT CCCAGCCCCG CCTATGTCCA AGCCCCTCAA GCCTCGGGGG CCAACCTCTT 
GGGGGCCCTG GGAGCCGGCC TAAGAAAAAA CTCAGCCCAC CCAGTATCTC TATAGACCCC 
CCGGAGAGCC AGGGCCCTCG GCCCCCATGC AGTCCTGGCG TCTGCCTCAG GAGGAGGGCG 
CCGGCCAGTG ACTCGAAGGA TCCCTCGGCC TCCAGCCCCC TTGACAGCAC GGCTGCCTCA 
CCCTCCCCAA AGAAAGATGC GCTGAGTCTC TCTGGTTTGT CTTCTGACCC AACAGACCTG 
GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532084.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532147.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X25, mRNA.

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CloneID	OMu45479
Clone ID Related Accession (Same CDS sequence)	XM_006532084.4 , XM_006532084.3
Accession Version	XM_006532084.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532084.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAT CTAATGTTGG ACGATGTAAT TGCTTCCGGC 
AGCTCAGCCA GCGCTGCGTC AGAAGCCCAG TGCAAACCCT ACTACTCTGA CTACTCGCGC 
TTCCGGCTCC TCGTCCACCA CCTGTGTACC AGCCACTACC TGGACCTCTT CATCACTGGT 
GTCATCGGGC TGAATGTGGT CACGATGGCC ATGGAACATT ACCAGCAGCC CCAGATCCTG 
GACGAGGCTC TGAAGATCTG CAACTACATC TTTACCGTCA TCTTTGTCTT GGAGTCAGTA 
TTCAAACTTG TGGCCTTCGG CTTCCGCCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCTATTG TGCTTCTGTC CATCATGGGC ATCACGCTGG AAGAGATTGA GGTCAATGCT 
TCACTGCCCA TCAACCCCAC CATCATCCGT ATCATGAGGG TGCTCCGCAT TGCTCGAGTT 
CTGAAGCTGT TGAAGATGGC TGTGGGCATG CGGGCACTGC TGGACACGGT GATGCAGGCC 
CTGCCCCAGG TGGGGAACCT GGGACTTCTC TTCATGCTAT TATTTTTCAT CTTTGCAGCT 
CTGGGCGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CACACCCTTG TGAGGGCTTG 
GGCCGGCATG CCACCTTTAG GAACTTTGGT ATGGCCTTTC TGACCCTCTT CCGAGTCTCC 
ACTGGTGACA ACTGGAATGG TATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACCTGCTACA ACACCGTCAT CTCACCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT CATAGCCGTG CTGATGAAGC ACCTGGAAGA GAGCAACAAA 
GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG GAGCTGGAGC TAGAGATGAA GACACTCAGC 
CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT 
AGCCCTGACA GCCCTAAGCC TGGGGCTCCA CACACCACGG CCCACATTGG AGCAGCCTCT 
TCAGGCTTCT CCCTTGAGCA CCCCACGATG GTACCTCACA CTGAGGAGGG GCCAGTCCCC 
CTAGGACCAG ACCTGCTGAC TGTGAGGAAG TCTGGTGTCA GCCGGACACA CTCTCTGCCC 
AATGACAGCT ACATGTGCCG CAATGGGAGC ACTGCCGAGA GATCCCTAGG ACACAGGGGC 
TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC ATCTTGTCTG TTCACTCCCA ACCAGCAGAC 
ACCAGCTGCA TCCTACAGCT TCCCAAAGAT GCACACTATC TGCTCCAGCC TCATGGGGCT 
CCCACCTGGG GCGCCATCCC TAAACTACCC CCACCTGGCC GCTCCCCTCT GGCTCAGAGG 
CCTCTCAGGC GCCAGGCAGC AATAAGGACT GACTCCCTGG ACGTGCAGGG CCTGGGTAGC 
CGGGAAGACC TGTTGTCAGA GGTGAGTGGG CCCTCCTGCC CTCTGACCCG CTCCTCATCC 
TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC 
AAGCACATCC GCCTGCCAGC CCCTTGCCCA GGCCTGGAAC CCAGCTGGGC CAAGGACCCT 
CAAGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC CCACGGGACT TGAAAAAATG CTACAGTGTA 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG TCCTGGCTAG ACGAACAGAG GAGACACTCC 
ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC CAGCCCCGCC TATGTCCAAG CCCCTCAAGC 
CTCGGGGGCC AACCTCTTGG GGGCCCTGGG AGCCGGCCTA AGAAAAAACT CAGCCCACCC 
AGTATCTCTA TAGACCCCCC GGAGAGCCAG GGCCCTCGGC CCCCATGCAG TCCTGGCGTC 
TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC TCGAAGGATC CCTCGGCCTC CAGCCCCCTT 
GACAGCACGG CTGCCTCACC CTCCCCAAAG AAAGATGCGC TGAGTCTCTC TGGTTTGTCT 
TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532147.1
CDS	784..7650
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532084.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532084.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAT CTAATGTTGG ACGATGTAAT TGCTTCCGGC 
AGCTCAGCCA GCGCTGCGTC AGAAGCCCAG TGCAAACCCT ACTACTCTGA CTACTCGCGC 
TTCCGGCTCC TCGTCCACCA CCTGTGTACC AGCCACTACC TGGACCTCTT CATCACTGGT 
GTCATCGGGC TGAATGTGGT CACGATGGCC ATGGAACATT ACCAGCAGCC CCAGATCCTG 
GACGAGGCTC TGAAGATCTG CAACTACATC TTTACCGTCA TCTTTGTCTT GGAGTCAGTA 
TTCAAACTTG TGGCCTTCGG CTTCCGCCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCTATTG TGCTTCTGTC CATCATGGGC ATCACGCTGG AAGAGATTGA GGTCAATGCT 
TCACTGCCCA TCAACCCCAC CATCATCCGT ATCATGAGGG TGCTCCGCAT TGCTCGAGTT 
CTGAAGCTGT TGAAGATGGC TGTGGGCATG CGGGCACTGC TGGACACGGT GATGCAGGCC 
CTGCCCCAGG TGGGGAACCT GGGACTTCTC TTCATGCTAT TATTTTTCAT CTTTGCAGCT 
CTGGGCGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CACACCCTTG TGAGGGCTTG 
GGCCGGCATG CCACCTTTAG GAACTTTGGT ATGGCCTTTC TGACCCTCTT CCGAGTCTCC 
ACTGGTGACA ACTGGAATGG TATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACCTGCTACA ACACCGTCAT CTCACCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT CATAGCCGTG CTGATGAAGC ACCTGGAAGA GAGCAACAAA 
GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG GAGCTGGAGC TAGAGATGAA GACACTCAGC 
CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT 
AGCCCTGACA GCCCTAAGCC TGGGGCTCCA CACACCACGG CCCACATTGG AGCAGCCTCT 
TCAGGCTTCT CCCTTGAGCA CCCCACGATG GTACCTCACA CTGAGGAGGG GCCAGTCCCC 
CTAGGACCAG ACCTGCTGAC TGTGAGGAAG TCTGGTGTCA GCCGGACACA CTCTCTGCCC 
AATGACAGCT ACATGTGCCG CAATGGGAGC ACTGCCGAGA GATCCCTAGG ACACAGGGGC 
TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC ATCTTGTCTG TTCACTCCCA ACCAGCAGAC 
ACCAGCTGCA TCCTACAGCT TCCCAAAGAT GCACACTATC TGCTCCAGCC TCATGGGGCT 
CCCACCTGGG GCGCCATCCC TAAACTACCC CCACCTGGCC GCTCCCCTCT GGCTCAGAGG 
CCTCTCAGGC GCCAGGCAGC AATAAGGACT GACTCCCTGG ACGTGCAGGG CCTGGGTAGC 
CGGGAAGACC TGTTGTCAGA GGTGAGTGGG CCCTCCTGCC CTCTGACCCG CTCCTCATCC 
TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC 
AAGCACATCC GCCTGCCAGC CCCTTGCCCA GGCCTGGAAC CCAGCTGGGC CAAGGACCCT 
CAAGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC CCACGGGACT TGAAAAAATG CTACAGTGTA 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG TCCTGGCTAG ACGAACAGAG GAGACACTCC 
ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC CAGCCCCGCC TATGTCCAAG CCCCTCAAGC 
CTCGGGGGCC AACCTCTTGG GGGCCCTGGG AGCCGGCCTA AGAAAAAACT CAGCCCACCC 
AGTATCTCTA TAGACCCCCC GGAGAGCCAG GGCCCTCGGC CCCCATGCAG TCCTGGCGTC 
TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC TCGAAGGATC CCTCGGCCTC CAGCCCCCTT 
GACAGCACGG CTGCCTCACC CTCCCCAAAG AAAGATGCGC TGAGTCTCTC TGGTTTGTCT 
TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532085.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532148.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X27, mRNA.

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CloneID	OMu45480
Clone ID Related Accession (Same CDS sequence)	XM_006532085.3 , XM_006532085.4
Accession Version	XM_006532085.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532085.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA AGCCCAGTGC 
AAACCCTACT ACTCTGACTA CTCGCGCTTC CGGCTCCTCG TCCACCACCT GTGTACCAGC 
CACTACCTGG ACCTCTTCAT CACTGGTGTC ATCGGGCTGA ATGTGGTCAC GATGGCCATG 
GAACATTACC AGCAGCCCCA GATCCTGGAC GAGGCTCTGA AGATCTGCAA CTACATCTTT 
ACCGTCATCT TTGTCTTGGA GTCAGTATTC AAACTTGTGG CCTTCGGCTT CCGCCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCTATTGTGC TTCTGTCCAT CATGGGCATC 
ACGCTGGAAG AGATTGAGGT CAATGCTTCA CTGCCCATCA ACCCCACCAT CATCCGTATC 
ATGAGGGTGC TCCGCATTGC TCGAGTTCTG AAGCTGTTGA AGATGGCTGT GGGCATGCGG 
GCACTGCTGG ACACGGTGAT GCAGGCCCTG CCCCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGCTATTAT TTTTCATCTT TGCAGCTCTG GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACAC ACCCTTGTGA GGGCTTGGGC CGGCATGCCA CCTTTAGGAA CTTTGGTATG 
GCCTTTCTGA CCCTCTTCCG AGTCTCCACT GGTGACAACT GGAATGGTAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACC TGCTACAACA CCGTCATCTC ACCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG 
ATGAAGCACC TGGAAGAGAG CAACAAAGAG GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG 
CTGGAGCTAG AGATGAAGAC ACTCAGCCCG CAGCCCCACT CCCCGCTGGG CAGCCCCTTC 
CTCTGGCCTG GGGTGGAAGG TGTCAATAGC CCTGACAGCC CTAAGCCTGG GGCTCCACAC 
ACCACGGCCC ACATTGGAGC AGCCTCTTCA GGCTTCTCCC TTGAGCACCC CACGATGGTA 
CCTCACACTG AGGAGGGGCC AGTCCCCCTA GGACCAGACC TGCTGACTGT GAGGAAGTCT 
GGTGTCAGCC GGACACACTC TCTGCCCAAT GACAGCTACA TGTGCCGCAA TGGGAGCACT 
GCCGAGAGAT CCCTAGGACA CAGGGGCTGG GGGCTCCCCA AAGCCCAGTC AGGCTCCATC 
TTGTCTGTTC ACTCCCAACC AGCAGACACC AGCTGCATCC TACAGCTTCC CAAAGATGCA 
CACTATCTGC TCCAGCCTCA TGGGGCTCCC ACCTGGGGCG CCATCCCTAA ACTACCCCCA 
CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT CTCAGGCGCC AGGCAGCAAT AAGGACTGAC 
TCCCTGGACG TGCAGGGCCT GGGTAGCCGG GAAGACCTGT TGTCAGAGGT GAGTGGGCCC 
TCCTGCCCTC TGACCCGCTC CTCATCCTTC TGGGGCGGGT CGAGCATCCA GGTGCAGCAG 
CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG CACATCCGCC TGCCAGCCCC TTGCCCAGGC 
CTGGAACCCA GCTGGGCCAA GGACCCTCAA GAGACCAGAA GCAGCTTAGA GCTGGACACG 
GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCAGCAGTC AGGAAGAACC CCTGTCCCCA 
CGGGACTTGA AAAAATGCTA CAGTGTAGAG GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC 
TGGCTAGACG AACAGAGGAG ACACTCCATC GCTGTCAGCT GCCTGGACAG CGGCTCCCAG 
CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC 
CGGCCTAAGA AAAAACTCAG CCCACCCAGT ATCTCTATAG ACCCCCCGGA GAGCCAGGGC 
CCTCGGCCCC CATGCAGTCC TGGCGTCTGC CTCAGGAGGA GGGCGCCGGC CAGTGACTCG 
AAGGATCCCT CGGCCTCCAG CCCCCTTGAC AGCACGGCTG CCTCACCCTC CCCAAAGAAA 
GATGCGCTGA GTCTCTCTGG TTTGTCTTCT GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532148.1
CDS	784..7617
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532085.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532085.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA AGCCCAGTGC 
AAACCCTACT ACTCTGACTA CTCGCGCTTC CGGCTCCTCG TCCACCACCT GTGTACCAGC 
CACTACCTGG ACCTCTTCAT CACTGGTGTC ATCGGGCTGA ATGTGGTCAC GATGGCCATG 
GAACATTACC AGCAGCCCCA GATCCTGGAC GAGGCTCTGA AGATCTGCAA CTACATCTTT 
ACCGTCATCT TTGTCTTGGA GTCAGTATTC AAACTTGTGG CCTTCGGCTT CCGCCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCTATTGTGC TTCTGTCCAT CATGGGCATC 
ACGCTGGAAG AGATTGAGGT CAATGCTTCA CTGCCCATCA ACCCCACCAT CATCCGTATC 
ATGAGGGTGC TCCGCATTGC TCGAGTTCTG AAGCTGTTGA AGATGGCTGT GGGCATGCGG 
GCACTGCTGG ACACGGTGAT GCAGGCCCTG CCCCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGCTATTAT TTTTCATCTT TGCAGCTCTG GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACAC ACCCTTGTGA GGGCTTGGGC CGGCATGCCA CCTTTAGGAA CTTTGGTATG 
GCCTTTCTGA CCCTCTTCCG AGTCTCCACT GGTGACAACT GGAATGGTAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACC TGCTACAACA CCGTCATCTC ACCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG 
ATGAAGCACC TGGAAGAGAG CAACAAAGAG GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG 
CTGGAGCTAG AGATGAAGAC ACTCAGCCCG CAGCCCCACT CCCCGCTGGG CAGCCCCTTC 
CTCTGGCCTG GGGTGGAAGG TGTCAATAGC CCTGACAGCC CTAAGCCTGG GGCTCCACAC 
ACCACGGCCC ACATTGGAGC AGCCTCTTCA GGCTTCTCCC TTGAGCACCC CACGATGGTA 
CCTCACACTG AGGAGGGGCC AGTCCCCCTA GGACCAGACC TGCTGACTGT GAGGAAGTCT 
GGTGTCAGCC GGACACACTC TCTGCCCAAT GACAGCTACA TGTGCCGCAA TGGGAGCACT 
GCCGAGAGAT CCCTAGGACA CAGGGGCTGG GGGCTCCCCA AAGCCCAGTC AGGCTCCATC 
TTGTCTGTTC ACTCCCAACC AGCAGACACC AGCTGCATCC TACAGCTTCC CAAAGATGCA 
CACTATCTGC TCCAGCCTCA TGGGGCTCCC ACCTGGGGCG CCATCCCTAA ACTACCCCCA 
CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT CTCAGGCGCC AGGCAGCAAT AAGGACTGAC 
TCCCTGGACG TGCAGGGCCT GGGTAGCCGG GAAGACCTGT TGTCAGAGGT GAGTGGGCCC 
TCCTGCCCTC TGACCCGCTC CTCATCCTTC TGGGGCGGGT CGAGCATCCA GGTGCAGCAG 
CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG CACATCCGCC TGCCAGCCCC TTGCCCAGGC 
CTGGAACCCA GCTGGGCCAA GGACCCTCAA GAGACCAGAA GCAGCTTAGA GCTGGACACG 
GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCAGCAGTC AGGAAGAACC CCTGTCCCCA 
CGGGACTTGA AAAAATGCTA CAGTGTAGAG GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC 
TGGCTAGACG AACAGAGGAG ACACTCCATC GCTGTCAGCT GCCTGGACAG CGGCTCCCAG 
CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC 
CGGCCTAAGA AAAAACTCAG CCCACCCAGT ATCTCTATAG ACCCCCCGGA GAGCCAGGGC 
CCTCGGCCCC CATGCAGTCC TGGCGTCTGC CTCAGGAGGA GGGCGCCGGC CAGTGACTCG 
AAGGATCCCT CGGCCTCCAG CCCCCTTGAC AGCACGGCTG CCTCACCCTC CCCAAAGAAA 
GATGCGCTGA GTCTCTCTGG TTTGTCTTCT GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532086.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532149.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X29, mRNA.

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CloneID	OMu45481
Clone ID Related Accession (Same CDS sequence)	XM_006532086.3 , XM_006532086.4
Accession Version	XM_006532086.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532086.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAA GCCCAGTGCA AACCCTACTA CTCTGACTAC 
TCGCGCTTCC GGCTCCTCGT CCACCACCTG TGTACCAGCC ACTACCTGGA CCTCTTCATC 
ACTGGTGTCA TCGGGCTGAA TGTGGTCACG ATGGCCATGG AACATTACCA GCAGCCCCAG 
ATCCTGGACG AGGCTCTGAA GATCTGCAAC TACATCTTTA CCGTCATCTT TGTCTTGGAG 
TCAGTATTCA AACTTGTGGC CTTCGGCTTC CGCCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CTATTGTGCT TCTGTCCATC ATGGGCATCA CGCTGGAAGA GATTGAGGTC 
AATGCTTCAC TGCCCATCAA CCCCACCATC ATCCGTATCA TGAGGGTGCT CCGCATTGCT 
CGAGTTCTGA AGCTGTTGAA GATGGCTGTG GGCATGCGGG CACTGCTGGA CACGGTGATG 
CAGGCCCTGC CCCAGGTGGG GAACCTGGGA CTTCTCTTCA TGCTATTATT TTTCATCTTT 
GCAGCTCTGG GCGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACACA CCCTTGTGAG 
GGCTTGGGCC GGCATGCCAC CTTTAGGAAC TTTGGTATGG CCTTTCTGAC CCTCTTCCGA 
GTCTCCACTG GTGACAACTG GAATGGTATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACCT GCTACAACAC CGTCATCTCA CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTCATA GCCGTGCTGA TGAAGCACCT GGAAGAGAGC 
AACAAAGAGG CCAAGGAGGA GGCGGAGTTG GAGGCGGAGC TGGAGCTAGA GATGAAGACA 
CTCAGCCCGC AGCCCCACTC CCCGCTGGGC AGCCCCTTCC TCTGGCCTGG GGTGGAAGGT 
GTCAATAGCC CTGACAGCCC TAAGCCTGGG GCTCCACACA CCACGGCCCA CATTGGAGCA 
GCCTCTTCAG GCTTCTCCCT TGAGCACCCC ACGATGGTAC CTCACACTGA GGAGGGGCCA 
GTCCCCCTAG GACCAGACCT GCTGACTGTG AGGAAGTCTG GTGTCAGCCG GACACACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGCAAT GGGAGCACTG CCGAGAGATC CCTAGGACAC 
AGGGGCTGGG GGCTCCCCAA AGCCCAGTCA GGCTCCATCT TGTCTGTTCA CTCCCAACCA 
GCAGACACCA GCTGCATCCT ACAGCTTCCC AAAGATGCAC ACTATCTGCT CCAGCCTCAT 
GGGGCTCCCA CCTGGGGCGC CATCCCTAAA CTACCCCCAC CTGGCCGCTC CCCTCTGGCT 
CAGAGGCCTC TCAGGCGCCA GGCAGCAATA AGGACTGACT CCCTGGACGT GCAGGGCCTG 
GGTAGCCGGG AAGACCTGTT GTCAGAGGTG AGTGGGCCCT CCTGCCCTCT GACCCGCTCC 
TCATCCTTCT GGGGCGGGTC GAGCATCCAG GTGCAGCAGC GCTCCGGCAG CCAGAGCAAA 
GTCTCCAAGC ACATCCGCCT GCCAGCCCCT TGCCCAGGCC TGGAACCCAG CTGGGCCAAG 
GACCCTCAAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCAGCAGTCA GGAAGAACCC CTGTCCCCAC GGGACTTGAA AAAATGCTAC 
AGTGTAGAGG CCCAGAGCTG CCGGCGCAGG CCTGGGTCCT GGCTAGACGA ACAGAGGAGA 
CACTCCATCG CTGTCAGCTG CCTGGACAGC GGCTCCCAGC CCCGCCTATG TCCAAGCCCC 
TCAAGCCTCG GGGGCCAACC TCTTGGGGGC CCTGGGAGCC GGCCTAAGAA AAAACTCAGC 
CCACCCAGTA TCTCTATAGA CCCCCCGGAG AGCCAGGGCC CTCGGCCCCC ATGCAGTCCT 
GGCGTCTGCC TCAGGAGGAG GGCGCCGGCC AGTGACTCGA AGGATCCCTC GGCCTCCAGC 
CCCCTTGACA GCACGGCTGC CTCACCCTCC CCAAAGAAAG ATGCGCTGAG TCTCTCTGGT 
TTGTCTTCTG ACCCAACAGA CCTGGATCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532149.1
CDS	784..7596
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532086.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532086.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAA GCCCAGTGCA AACCCTACTA CTCTGACTAC 
TCGCGCTTCC GGCTCCTCGT CCACCACCTG TGTACCAGCC ACTACCTGGA CCTCTTCATC 
ACTGGTGTCA TCGGGCTGAA TGTGGTCACG ATGGCCATGG AACATTACCA GCAGCCCCAG 
ATCCTGGACG AGGCTCTGAA GATCTGCAAC TACATCTTTA CCGTCATCTT TGTCTTGGAG 
TCAGTATTCA AACTTGTGGC CTTCGGCTTC CGCCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CTATTGTGCT TCTGTCCATC ATGGGCATCA CGCTGGAAGA GATTGAGGTC 
AATGCTTCAC TGCCCATCAA CCCCACCATC ATCCGTATCA TGAGGGTGCT CCGCATTGCT 
CGAGTTCTGA AGCTGTTGAA GATGGCTGTG GGCATGCGGG CACTGCTGGA CACGGTGATG 
CAGGCCCTGC CCCAGGTGGG GAACCTGGGA CTTCTCTTCA TGCTATTATT TTTCATCTTT 
GCAGCTCTGG GCGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACACA CCCTTGTGAG 
GGCTTGGGCC GGCATGCCAC CTTTAGGAAC TTTGGTATGG CCTTTCTGAC CCTCTTCCGA 
GTCTCCACTG GTGACAACTG GAATGGTATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACCT GCTACAACAC CGTCATCTCA CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTCATA GCCGTGCTGA TGAAGCACCT GGAAGAGAGC 
AACAAAGAGG CCAAGGAGGA GGCGGAGTTG GAGGCGGAGC TGGAGCTAGA GATGAAGACA 
CTCAGCCCGC AGCCCCACTC CCCGCTGGGC AGCCCCTTCC TCTGGCCTGG GGTGGAAGGT 
GTCAATAGCC CTGACAGCCC TAAGCCTGGG GCTCCACACA CCACGGCCCA CATTGGAGCA 
GCCTCTTCAG GCTTCTCCCT TGAGCACCCC ACGATGGTAC CTCACACTGA GGAGGGGCCA 
GTCCCCCTAG GACCAGACCT GCTGACTGTG AGGAAGTCTG GTGTCAGCCG GACACACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGCAAT GGGAGCACTG CCGAGAGATC CCTAGGACAC 
AGGGGCTGGG GGCTCCCCAA AGCCCAGTCA GGCTCCATCT TGTCTGTTCA CTCCCAACCA 
GCAGACACCA GCTGCATCCT ACAGCTTCCC AAAGATGCAC ACTATCTGCT CCAGCCTCAT 
GGGGCTCCCA CCTGGGGCGC CATCCCTAAA CTACCCCCAC CTGGCCGCTC CCCTCTGGCT 
CAGAGGCCTC TCAGGCGCCA GGCAGCAATA AGGACTGACT CCCTGGACGT GCAGGGCCTG 
GGTAGCCGGG AAGACCTGTT GTCAGAGGTG AGTGGGCCCT CCTGCCCTCT GACCCGCTCC 
TCATCCTTCT GGGGCGGGTC GAGCATCCAG GTGCAGCAGC GCTCCGGCAG CCAGAGCAAA 
GTCTCCAAGC ACATCCGCCT GCCAGCCCCT TGCCCAGGCC TGGAACCCAG CTGGGCCAAG 
GACCCTCAAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCAGCAGTCA GGAAGAACCC CTGTCCCCAC GGGACTTGAA AAAATGCTAC 
AGTGTAGAGG CCCAGAGCTG CCGGCGCAGG CCTGGGTCCT GGCTAGACGA ACAGAGGAGA 
CACTCCATCG CTGTCAGCTG CCTGGACAGC GGCTCCCAGC CCCGCCTATG TCCAAGCCCC 
TCAAGCCTCG GGGGCCAACC TCTTGGGGGC CCTGGGAGCC GGCCTAAGAA AAAACTCAGC 
CCACCCAGTA TCTCTATAGA CCCCCCGGAG AGCCAGGGCC CTCGGCCCCC ATGCAGTCCT 
GGCGTCTGCC TCAGGAGGAG GGCGCCGGCC AGTGACTCGA AGGATCCCTC GGCCTCCAGC 
CCCCTTGACA GCACGGCTGC CTCACCCTCC CCAAAGAAAG ATGCGCTGAG TCTCTCTGGT 
TTGTCTTCTG ACCCAACAGA CCTGGATCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532087.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532150.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X31, mRNA.

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CloneID	OMu45482
Clone ID Related Accession (Same CDS sequence)	XM_006532087.3 , XM_006532087.4
Accession Version	XM_006532087.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532087.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGATGGT ACCTCACACT 
GAGGAGGGGC CAGTCCCCCT AGGACCAGAC CTGCTGACTG TGAGGAAGTC TGGTGTCAGC 
CGGACACACT CTCTGCCCAA TGACAGCTAC ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA 
TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT 
CACTCCCAAC CAGCAGACAC CAGCTGCATC CTACAGCTTC CCAAAGATGC ACACTATCTG 
CTCCAGCCTC ATGGGGCTCC CACCTGGGGC GCCATCCCTA AACTACCCCC ACCTGGCCGC 
TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC CAGGCAGCAA TAAGGACTGA CTCCCTGGAC 
GTGCAGGGCC TGGGTAGCCG GGAAGACCTG TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT 
CTGACCCGCT CCTCATCCTT CTGGGGCGGG TCGAGCATCC AGGTGCAGCA GCGCTCCGGC 
AGCCAGAGCA AAGTCTCCAA GCACATCCGC CTGCCAGCCC CTTGCCCAGG CCTGGAACCC 
AGCTGGGCCA AGGACCCTCA AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCAGCAGT CAGGAAGAAC CCCTGTCCCC ACGGGACTTG 
AAAAAATGCT ACAGTGTAGA GGCCCAGAGC TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC 
GAACAGAGGA GACACTCCAT CGCTGTCAGC TGCCTGGACA GCGGCTCCCA GCCCCGCCTA 
TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG 
AAAAAACTCA GCCCACCCAG TATCTCTATA GACCCCCCGG AGAGCCAGGG CCCTCGGCCC 
CCATGCAGTC CTGGCGTCTG CCTCAGGAGG AGGGCGCCGG CCAGTGACTC GAAGGATCCC 
TCGGCCTCCA GCCCCCTTGA CAGCACGGCT GCCTCACCCT CCCCAAAGAA AGATGCGCTG 
AGTCTCTCTG GTTTGTCTTC TGACCCAACA GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532150.1
CDS	784..7548
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532087.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532087.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGATGGT ACCTCACACT 
GAGGAGGGGC CAGTCCCCCT AGGACCAGAC CTGCTGACTG TGAGGAAGTC TGGTGTCAGC 
CGGACACACT CTCTGCCCAA TGACAGCTAC ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA 
TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT 
CACTCCCAAC CAGCAGACAC CAGCTGCATC CTACAGCTTC CCAAAGATGC ACACTATCTG 
CTCCAGCCTC ATGGGGCTCC CACCTGGGGC GCCATCCCTA AACTACCCCC ACCTGGCCGC 
TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC CAGGCAGCAA TAAGGACTGA CTCCCTGGAC 
GTGCAGGGCC TGGGTAGCCG GGAAGACCTG TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT 
CTGACCCGCT CCTCATCCTT CTGGGGCGGG TCGAGCATCC AGGTGCAGCA GCGCTCCGGC 
AGCCAGAGCA AAGTCTCCAA GCACATCCGC CTGCCAGCCC CTTGCCCAGG CCTGGAACCC 
AGCTGGGCCA AGGACCCTCA AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCAGCAGT CAGGAAGAAC CCCTGTCCCC ACGGGACTTG 
AAAAAATGCT ACAGTGTAGA GGCCCAGAGC TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC 
GAACAGAGGA GACACTCCAT CGCTGTCAGC TGCCTGGACA GCGGCTCCCA GCCCCGCCTA 
TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG 
AAAAAACTCA GCCCACCCAG TATCTCTATA GACCCCCCGG AGAGCCAGGG CCCTCGGCCC 
CCATGCAGTC CTGGCGTCTG CCTCAGGAGG AGGGCGCCGG CCAGTGACTC GAAGGATCCC 
TCGGCCTCCA GCCCCCTTGA CAGCACGGCT GCCTCACCCT CCCCAAAGAA AGATGCGCTG 
AGTCTCTCTG GTTTGTCTTC TGACCCAACA GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532088.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532151.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X32, mRNA.

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CloneID	OMu45483
Clone ID Related Accession (Same CDS sequence)	XM_006532088.4 , XM_006532088.3
Accession Version	XM_006532088.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532088.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCTGACTA CTCGCGCTTC 
CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ACCTCTTCAT CACTGGTGTC 
ATCGGGCTGA ATGTGGTCAC GATGGCCATG GAACATTACC AGCAGCCCCA GATCCTGGAC 
GAGGCTCTGA AGATCTGCAA CTACATCTTT ACCGTCATCT TTGTCTTGGA GTCAGTATTC 
AAACTTGTGG CCTTCGGCTT CCGCCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCTATTGTGC TTCTGTCCAT CATGGGCATC ACGCTGGAAG AGATTGAGGT CAATGCTTCA 
CTGCCCATCA ACCCCACCAT CATCCGTATC ATGAGGGTGC TCCGCATTGC TCGAGTTCTG 
AAGCTGTTGA AGATGGCTGT GGGCATGCGG GCACTGCTGG ACACGGTGAT GCAGGCCCTG 
CCCCAGGTGG GGAACCTGGG ACTTCTCTTC ATGCTATTAT TTTTCATCTT TGCAGCTCTG 
GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACAC ACCCTTGTGA GGGCTTGGGC 
CGGCATGCCA CCTTTAGGAA CTTTGGTATG GCCTTTCTGA CCCTCTTCCG AGTCTCCACT 
GGTGACAACT GGAATGGTAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC 
TGCTACAACA CCGTCATCTC ACCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG ATGAAGCACC TGGAAGAGAG CAACAAAGAG 
GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG CTGGAGCTAG AGATGAAGAC ACTCAGCCCG 
CAGCCCCACT CCCCGCTGGG CAGCCCCTTC CTCTGGCCTG GGGTGGAAGG TGTCAATAGC 
CCTGACAGCC CTAAGCCTGG GGCTCCACAC ACCACGGCCC ACATTGGAGC AGCCTCTTCA 
GGCTTCTCCC TTGAGCACCC CACGATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532151.1
CDS	784..7527
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532088.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532088.4

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCTGACTA CTCGCGCTTC 
CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ACCTCTTCAT CACTGGTGTC 
ATCGGGCTGA ATGTGGTCAC GATGGCCATG GAACATTACC AGCAGCCCCA GATCCTGGAC 
GAGGCTCTGA AGATCTGCAA CTACATCTTT ACCGTCATCT TTGTCTTGGA GTCAGTATTC 
AAACTTGTGG CCTTCGGCTT CCGCCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCTATTGTGC TTCTGTCCAT CATGGGCATC ACGCTGGAAG AGATTGAGGT CAATGCTTCA 
CTGCCCATCA ACCCCACCAT CATCCGTATC ATGAGGGTGC TCCGCATTGC TCGAGTTCTG 
AAGCTGTTGA AGATGGCTGT GGGCATGCGG GCACTGCTGG ACACGGTGAT GCAGGCCCTG 
CCCCAGGTGG GGAACCTGGG ACTTCTCTTC ATGCTATTAT TTTTCATCTT TGCAGCTCTG 
GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACAC ACCCTTGTGA GGGCTTGGGC 
CGGCATGCCA CCTTTAGGAA CTTTGGTATG GCCTTTCTGA CCCTCTTCCG AGTCTCCACT 
GGTGACAACT GGAATGGTAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC 
TGCTACAACA CCGTCATCTC ACCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG ATGAAGCACC TGGAAGAGAG CAACAAAGAG 
GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG CTGGAGCTAG AGATGAAGAC ACTCAGCCCG 
CAGCCCCACT CCCCGCTGGG CAGCCCCTTC CTCTGGCCTG GGGTGGAAGG TGTCAATAGC 
CCTGACAGCC CTAAGCCTGG GGCTCCACAC ACCACGGCCC ACATTGGAGC AGCCTCTTCA 
GGCTTCTCCC TTGAGCACCC CACGATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248686.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246988.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X1, mRNA.

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CloneID	OMu67268
Clone ID Related Accession (Same CDS sequence)	XM_011248686.3 , XM_011248686.2
Accession Version	XM_011248686.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248686.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
GACAGACAGC TGTTTGACAC CATCTCCCTG CTGATCCAGG GCTCCCTGGA GGGGGAGCTG 
AAGCTGATGG ACGAGCTGGC AGGCCCAGGG GGCCAGCCCT CTGCCTTCCC TTCCGCCCCC 
AGCCCGGGCG ACTCCGACCC ACAGATCCCT CTAGCTGAGA TGGAGGCTCT GTCTCTGACG 
TCAGAGATTG TGTCTGAACC GTCCTGCTCT CTAGCTCTGA CGGATGACTC TTTGCCTGAT 
GACACTCACA CACTCTTACT TAGTGCCCTG GAGAGCAATA TGGTACCTCA CACTGAGGAG 
GGGCCAGTCC CCCTAGGACC AGACCTGCTG ACTGTGAGGA AGTCTGGTGT CAGCCGGACA 
CACTCTCTGC CCAATGACAG CTACATGTGC CGCAATGGGA GCACTGCCGA GAGATCCCTA 
GGACACAGGG GCTGGGGGCT CCCCAAAGCC CAGTCAGGCT CCATCTTGTC TGTTCACTCC 
CAACCAGCAG ACACCAGCTG CATCCTACAG CTTCCCAAAG ATGCACACTA TCTGCTCCAG 
CCTCATGGGG CTCCCACCTG GGGCGCCATC CCTAAACTAC CCCCACCTGG CCGCTCCCCT 
CTGGCTCAGA GGCCTCTCAG GCGCCAGGCA GCAATAAGGA CTGACTCCCT GGACGTGCAG 
GGCCTGGGTA GCCGGGAAGA CCTGTTGTCA GAGGTGAGTG GGCCCTCCTG CCCTCTGACC 
CGCTCCTCAT CCTTCTGGGG CGGGTCGAGC ATCCAGGTGC AGCAGCGCTC CGGCAGCCAG 
AGCAAAGTCT CCAAGCACAT CCGCCTGCCA GCCCCTTGCC CAGGCCTGGA ACCCAGCTGG 
GCCAAGGACC CTCAAGAGAC CAGAAGCAGC TTAGAGCTGG ACACGGAGCT GAGCTGGATT 
TCAGGAGACC TCCTGCCCAG CAGTCAGGAA GAACCCCTGT CCCCACGGGA CTTGAAAAAA 
TGCTACAGTG TAGAGGCCCA GAGCTGCCGG CGCAGGCCTG GGTCCTGGCT AGACGAACAG 
AGGAGACACT CCATCGCTGT CAGCTGCCTG GACAGCGGCT CCCAGCCCCG CCTATGTCCA 
AGCCCCTCAA GCCTCGGGGG CCAACCTCTT GGGGGCCCTG GGAGCCGGCC TAAGAAAAAA 
CTCAGCCCAC CCAGTATCTC TATAGACCCC CCGGAGAGCC AGGGCCCTCG GCCCCCATGC 
AGTCCTGGCG TCTGCCTCAG GAGGAGGGCG CCGGCCAGTG ACTCGAAGGA TCCCTCGGCC 
TCCAGCCCCC TTGACAGCAC GGCTGCCTCA CCCTCCCCAA AGAAAGATGC GCTGAGTCTC 
TCTGGTTTGT CTTCTGACCC AACAGACCTG GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246988.1
CDS	784..7962
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248686.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248686.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
GACAGACAGC TGTTTGACAC CATCTCCCTG CTGATCCAGG GCTCCCTGGA GGGGGAGCTG 
AAGCTGATGG ACGAGCTGGC AGGCCCAGGG GGCCAGCCCT CTGCCTTCCC TTCCGCCCCC 
AGCCCGGGCG ACTCCGACCC ACAGATCCCT CTAGCTGAGA TGGAGGCTCT GTCTCTGACG 
TCAGAGATTG TGTCTGAACC GTCCTGCTCT CTAGCTCTGA CGGATGACTC TTTGCCTGAT 
GACACTCACA CACTCTTACT TAGTGCCCTG GAGAGCAATA TGGTACCTCA CACTGAGGAG 
GGGCCAGTCC CCCTAGGACC AGACCTGCTG ACTGTGAGGA AGTCTGGTGT CAGCCGGACA 
CACTCTCTGC CCAATGACAG CTACATGTGC CGCAATGGGA GCACTGCCGA GAGATCCCTA 
GGACACAGGG GCTGGGGGCT CCCCAAAGCC CAGTCAGGCT CCATCTTGTC TGTTCACTCC 
CAACCAGCAG ACACCAGCTG CATCCTACAG CTTCCCAAAG ATGCACACTA TCTGCTCCAG 
CCTCATGGGG CTCCCACCTG GGGCGCCATC CCTAAACTAC CCCCACCTGG CCGCTCCCCT 
CTGGCTCAGA GGCCTCTCAG GCGCCAGGCA GCAATAAGGA CTGACTCCCT GGACGTGCAG 
GGCCTGGGTA GCCGGGAAGA CCTGTTGTCA GAGGTGAGTG GGCCCTCCTG CCCTCTGACC 
CGCTCCTCAT CCTTCTGGGG CGGGTCGAGC ATCCAGGTGC AGCAGCGCTC CGGCAGCCAG 
AGCAAAGTCT CCAAGCACAT CCGCCTGCCA GCCCCTTGCC CAGGCCTGGA ACCCAGCTGG 
GCCAAGGACC CTCAAGAGAC CAGAAGCAGC TTAGAGCTGG ACACGGAGCT GAGCTGGATT 
TCAGGAGACC TCCTGCCCAG CAGTCAGGAA GAACCCCTGT CCCCACGGGA CTTGAAAAAA 
TGCTACAGTG TAGAGGCCCA GAGCTGCCGG CGCAGGCCTG GGTCCTGGCT AGACGAACAG 
AGGAGACACT CCATCGCTGT CAGCTGCCTG GACAGCGGCT CCCAGCCCCG CCTATGTCCA 
AGCCCCTCAA GCCTCGGGGG CCAACCTCTT GGGGGCCCTG GGAGCCGGCC TAAGAAAAAA 
CTCAGCCCAC CCAGTATCTC TATAGACCCC CCGGAGAGCC AGGGCCCTCG GCCCCCATGC 
AGTCCTGGCG TCTGCCTCAG GAGGAGGGCG CCGGCCAGTG ACTCGAAGGA TCCCTCGGCC 
TCCAGCCCCC TTGACAGCAC GGCTGCCTCA CCCTCCCCAA AGAAAGATGC GCTGAGTCTC 
TCTGGTTTGT CTTCTGACCC AACAGACCTG GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248687.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246989.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X3, mRNA.

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CloneID	OMu67269
Clone ID Related Accession (Same CDS sequence)	XM_011248687.3 , XM_011248687.2
Accession Version	XM_011248687.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248687.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA ATCTAATGTT GGACGATGTA 
ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG TCAGAAGCCC AGTGCAAACC CTACTACTCT 
GACTACTCGC GCTTCCGGCT CCTCGTCCAC CACCTGTGTA CCAGCCACTA CCTGGACCTC 
TTCATCACTG GTGTCATCGG GCTGAATGTG GTCACGATGG CCATGGAACA TTACCAGCAG 
CCCCAGATCC TGGACGAGGC TCTGAAGATC TGCAACTACA TCTTTACCGT CATCTTTGTC 
TTGGAGTCAG TATTCAAACT TGTGGCCTTC GGCTTCCGCC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCTAT TGTGCTTCTG TCCATCATGG GCATCACGCT GGAAGAGATT 
GAGGTCAATG CTTCACTGCC CATCAACCCC ACCATCATCC GTATCATGAG GGTGCTCCGC 
ATTGCTCGAG TTCTGAAGCT GTTGAAGATG GCTGTGGGCA TGCGGGCACT GCTGGACACG 
GTGATGCAGG CCCTGCCCCA GGTGGGGAAC CTGGGACTTC TCTTCATGCT ATTATTTTTC 
ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACACACCCT 
TGTGAGGGCT TGGGCCGGCA TGCCACCTTT AGGAACTTTG GTATGGCCTT TCTGACCCTC 
TTCCGAGTCT CCACTGGTGA CAACTGGAAT GGTATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACCTGCTA CAACACCGTC ATCTCACCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTCATAGCCG TGCTGATGAA GCACCTGGAA 
GAGAGCAACA AAGAGGCCAA GGAGGAGGCG GAGTTGGAGG CGGAGCTGGA GCTAGAGATG 
AAGACACTCA GCCCGCAGCC CCACTCCCCG CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG 
GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG CCTGGGGCTC CACACACCAC GGCCCACATT 
GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG CACCCCACGG ACAGACAGCT GTTTGACACC 
ATCTCCCTGC TGATCCAGGG CTCCCTGGAG GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT TCCGCCCCCA GCCCGGGCGA CTCCGACCCA 
CAGATCCCTC TAGCTGAGAT GGAGGCTCTG TCTCTGACGT CAGAGATTGT GTCTGAACCG 
TCCTGCTCTC TAGCTCTGAC GGATGACTCT TTGCCTGATG ACACTCACAC ACTCTTACTT 
AGTGCCCTGG AGAGCAATAT GGTACCTCAC ACTGAGGAGG GGCCAGTCCC CCTAGGACCA 
GACCTGCTGA CTGTGAGGAA GTCTGGTGTC AGCCGGACAC ACTCTCTGCC CAATGACAGC 
TACATGTGCC GCAATGGGAG CACTGCCGAG AGATCCCTAG GACACAGGGG CTGGGGGCTC 
CCCAAAGCCC AGTCAGGCTC CATCTTGTCT GTTCACTCCC AACCAGCAGA CACCAGCTGC 
ATCCTACAGC TTCCCAAAGA TGCACACTAT CTGCTCCAGC CTCATGGGGC TCCCACCTGG 
GGCGCCATCC CTAAACTACC CCCACCTGGC CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG 
CGCCAGGCAG CAATAAGGAC TGACTCCCTG GACGTGCAGG GCCTGGGTAG CCGGGAAGAC 
CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC CCTCTGACCC GCTCCTCATC CTTCTGGGGC 
GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC GGCAGCCAGA GCAAAGTCTC CAAGCACATC 
CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA CCCAGCTGGG CCAAGGACCC TCAAGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGCCCAGC 
AGTCAGGAAG AACCCCTGTC CCCACGGGAC TTGAAAAAAT GCTACAGTGT AGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA GACGAACAGA GGAGACACTC CATCGCTGTC 
AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC CTATGTCCAA GCCCCTCAAG CCTCGGGGGC 
CAACCTCTTG GGGGCCCTGG GAGCCGGCCT AAGAAAAAAC TCAGCCCACC CAGTATCTCT 
ATAGACCCCC CGGAGAGCCA GGGCCCTCGG CCCCCATGCA GTCCTGGCGT CTGCCTCAGG 
AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT CCCTCGGCCT CCAGCCCCCT TGACAGCACG 
GCTGCCTCAC CCTCCCCAAA GAAAGATGCG CTGAGTCTCT CTGGTTTGTC TTCTGACCCA 
ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246989.1
CDS	784..7941
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248687.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248687.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA ATCTAATGTT GGACGATGTA 
ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG TCAGAAGCCC AGTGCAAACC CTACTACTCT 
GACTACTCGC GCTTCCGGCT CCTCGTCCAC CACCTGTGTA CCAGCCACTA CCTGGACCTC 
TTCATCACTG GTGTCATCGG GCTGAATGTG GTCACGATGG CCATGGAACA TTACCAGCAG 
CCCCAGATCC TGGACGAGGC TCTGAAGATC TGCAACTACA TCTTTACCGT CATCTTTGTC 
TTGGAGTCAG TATTCAAACT TGTGGCCTTC GGCTTCCGCC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCTAT TGTGCTTCTG TCCATCATGG GCATCACGCT GGAAGAGATT 
GAGGTCAATG CTTCACTGCC CATCAACCCC ACCATCATCC GTATCATGAG GGTGCTCCGC 
ATTGCTCGAG TTCTGAAGCT GTTGAAGATG GCTGTGGGCA TGCGGGCACT GCTGGACACG 
GTGATGCAGG CCCTGCCCCA GGTGGGGAAC CTGGGACTTC TCTTCATGCT ATTATTTTTC 
ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACACACCCT 
TGTGAGGGCT TGGGCCGGCA TGCCACCTTT AGGAACTTTG GTATGGCCTT TCTGACCCTC 
TTCCGAGTCT CCACTGGTGA CAACTGGAAT GGTATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACCTGCTA CAACACCGTC ATCTCACCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTCATAGCCG TGCTGATGAA GCACCTGGAA 
GAGAGCAACA AAGAGGCCAA GGAGGAGGCG GAGTTGGAGG CGGAGCTGGA GCTAGAGATG 
AAGACACTCA GCCCGCAGCC CCACTCCCCG CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG 
GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG CCTGGGGCTC CACACACCAC GGCCCACATT 
GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG CACCCCACGG ACAGACAGCT GTTTGACACC 
ATCTCCCTGC TGATCCAGGG CTCCCTGGAG GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT TCCGCCCCCA GCCCGGGCGA CTCCGACCCA 
CAGATCCCTC TAGCTGAGAT GGAGGCTCTG TCTCTGACGT CAGAGATTGT GTCTGAACCG 
TCCTGCTCTC TAGCTCTGAC GGATGACTCT TTGCCTGATG ACACTCACAC ACTCTTACTT 
AGTGCCCTGG AGAGCAATAT GGTACCTCAC ACTGAGGAGG GGCCAGTCCC CCTAGGACCA 
GACCTGCTGA CTGTGAGGAA GTCTGGTGTC AGCCGGACAC ACTCTCTGCC CAATGACAGC 
TACATGTGCC GCAATGGGAG CACTGCCGAG AGATCCCTAG GACACAGGGG CTGGGGGCTC 
CCCAAAGCCC AGTCAGGCTC CATCTTGTCT GTTCACTCCC AACCAGCAGA CACCAGCTGC 
ATCCTACAGC TTCCCAAAGA TGCACACTAT CTGCTCCAGC CTCATGGGGC TCCCACCTGG 
GGCGCCATCC CTAAACTACC CCCACCTGGC CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG 
CGCCAGGCAG CAATAAGGAC TGACTCCCTG GACGTGCAGG GCCTGGGTAG CCGGGAAGAC 
CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC CCTCTGACCC GCTCCTCATC CTTCTGGGGC 
GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC GGCAGCCAGA GCAAAGTCTC CAAGCACATC 
CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA CCCAGCTGGG CCAAGGACCC TCAAGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGCCCAGC 
AGTCAGGAAG AACCCCTGTC CCCACGGGAC TTGAAAAAAT GCTACAGTGT AGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA GACGAACAGA GGAGACACTC CATCGCTGTC 
AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC CTATGTCCAA GCCCCTCAAG CCTCGGGGGC 
CAACCTCTTG GGGGCCCTGG GAGCCGGCCT AAGAAAAAAC TCAGCCCACC CAGTATCTCT 
ATAGACCCCC CGGAGAGCCA GGGCCCTCGG CCCCCATGCA GTCCTGGCGT CTGCCTCAGG 
AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT CCCTCGGCCT CCAGCCCCCT TGACAGCACG 
GCTGCCTCAC CCTCCCCAAA GAAAGATGCG CTGAGTCTCT CTGGTTTGTC TTCTGACCCA 
ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248688.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246990.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X5, mRNA.

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CloneID	OMu67270
Clone ID Related Accession (Same CDS sequence)	XM_011248688.2 , XM_011248688.3
Accession Version	XM_011248688.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248688.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
TTCTCTGAGA CCAAGCAACG GGAGAGTCAG CTGATGCGGG AGCAGCGTGT ACGATTCCTG 
TCCAATGCTA GCACCCTGGC AAGCTTCTCT GAGCCAGGCA GCTGCTATGA GGAGCTTCTC 
AAGTACCTGG TGTACATCCT CCGCAAAGCA GCCCGCAGGC TGGCCCAGGT CTCTAGGGCT 
GTAGGCGTGC GGGCTGGGTT GCTCAGCAGC CCAGTGGCCC GTGGTGGGCA GGAGCCCCAG 
CCCAGTGGCA GCTGCTCTCG TTCACACCGT CGTCTGTCTG TCCACCACCT GGTCCACCAC 
CATCACCACC ACCATCACCA CTACCACCTG GGTAATGGGA CGCTCAGAGT TCCCCGGGCC 
AGCCCAGAGA TCCAGGACAG GGATGCCAAT GGGTCCCGCT GGCTCATGCT GCCACCACCC 
TCTACCCCCA CTCCCTCTGG GGGCCCTCCG AGGGGTGCGG AGTCTGTACA CAGCTTCTAC 
CATGCTGACT GCCACTTGGA GCCAGTCCGT TGCCAGGCGC CCCCTCCCAG GTCCCCATCG 
GAGGCATCTG GCAGGACTGT GGGTAGTGGG AAGGTATACC CCACTGTGCA TACCAGCCCT 
CCACCAGAGA TGCTGAAGGA TAAGGCACTA GTGGAGGTGG CCCCCAGCCC TGGGCCCCCC 
ACCCTCACCA GCTTCAACAT CCCACCTGGG CCCTTCAGCT CCATGCACAA GCTCCTGGAG 
ACACAGAGTA CGGGTGAGAG CGTGGGAGCC TGCCATAGCT CCTGCAAAAT CTCCAGCCCT 
TGCTCCAAGG CAGACAGTGG AGCCTGTGGG CCGGACAGTT GTCCCTACTG TGCCCGGACA 
GGAGCAGGGG AGCCAGAGTC CGCTGACCAT GAAATGCCTG ACTCAGACAG TGAGGCTGTG 
TATGAGTTCA CACAGGACGC TCAGCACAGC GACCTCCGGG ATCCCCACAG ACGGCGACGG 
CCGAGCCTGG GCCCAGATGC AGAGCCTAGT TCTGTGCTGG CCTTCTGGAG GCTGATCTGC 
GACACATTCC GGAAGATCGT AGATAGCAAA TACTTTGGCC GGGGAATCAT GATCGCTATC 
CTGGTCAATA CCCTCAGCAT GGGCATCGAG TACCACGAGC AGCCCGAGGA GCTCACCAAC 
GCCCTGGAAA TCAGCAACAT CGTCTTCACC AGCCTCTTCG CCTTGGAGAT GCTGCTGAAG 
CTGCTCGTCT ACGGTCCCTT CGGCTACATT AAGAATCCCT ACAACATCTT TGATGGCGTC 
ATTGTGGTCA TCAGCGTGTG GGAGATTGTG GGCCAGCAGG GAGGTGGCCT GTCGGTGCTG 
CGGACCTTCC GCCTGATGCG GGTGCTGAAG CTAGTTCGCT TCCTGCCGGC ACTGCAGCGG 
CAGCTCGTGG TGCTCATGAA GACCATGGAC AACGTGGCCA CCTTCTGCAT GCTGCTCATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTTT TTGGTTGCAA GTTCGCATCT 
GAACGGGATG GGGACACGTT GCCAGACCGG AAGAATTTTG ACTCCCTGCT CTGGGCCATT 
GTCACTGTCT TTCAGATTCT GACTCAGGAA GACTGGAATA AAGTCCTTTA CAACGGCATG 
GCCTCCACGT CATCTTGGGC TGCTCTTTAC TTCATCGCCC TCATGACTTT TGGCAACTAC 
GTGCTCTTTA ACCTGCTTGT CGCCATTCTC GTGGAGGGTT TCCAGGCAGA GGAAATCGGC 
AAACGGGAAG ATACGAGTGG ACAGTTAAGC TGTATTCAGC TGCCTGTCAA CTCCCAGGGG 
GGAGATGCCA CCAAGTCTGA GTCAGAGCCT GATTTCTTTT CGCCCAGTGT GGATGGTGAT 
GGGGACAGGA AGAAGCGCTT GGCCCTGGTG GCCTTGGGAG AACACTCGGA ACTACGAAAG 
AGCCTTTTGC CACCTCTCAT CATCCACACA GCTGCTACAC CGATGTCACT GCCCAAGAGC 
TCCAGCACAG GTGTGGGGGA AGCACTGGGC TCTGGCTCTC GCCGCACCAG TAGCAGTGGG 
TCCGCTGAGC CTGGAACTGC CCATCATGAG ATGAAATCAC CGCCAAGTGC CCGAAGCTCC 
CCGCACAGTC CCTGGAGCGC AGCAAGCAGC TGGACCAGCA GGCGCTCCAG CCGGAACAGC 
CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG 
TCTGGAGAGG GTCAGGAGAG CCAGGATGAG GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC 
CCAGCAGGCA GTGATCATCG CCACAGGGGT TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT 
GACCTGCCTG ACACCCTGCA GGTGCCCGGG CTTCATCGAA CAGCCAGCGG TCGGAGCTCT 
GCCTCTGAAC ACCAAGACTG TAATGGCAAG TCGGCTTCAG GGCGTTTGGC CCGCACCCTG 
CGGGCTGATG ACCCCCCACT GGATGGGGAT GATGGCGATG ATGAGGGCAA CCTGAGCAAA 
GGGGAACGCT TACGAGCCTG GGTCCGAGCC CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT 
TCCTGGTCTG CCTATATCTT CCCTCCCCAG TCAAGGTTTC GTCTCCTGTG TCACCGGATC 
ATCACCCACA AGATGTTTGA CCATGTGGTC CTCGTCATCA TCTTCCTCAA CTGTATCACC 
ATCGCTATGG AGCGCCCCAA AATTGACCCC CACAGCGCTG AACGCATCTT CCTGACCCTC 
TCCAACTACA TCTTCACGGC AGTCTTCCTG GCTGAAATGA CAGTGAAGGT GGTGGCACTG 
GGCTGGTGCT TTGGGGAGCA GGCCTACCTG CGCAGCAGCT GGAACGTGCT GGACGGCTTG 
CTGGTGCTCA TCTCTGTCAT CGACATCCTG GTGTCCATGG TCTCTGACAG CGGCACCAAG 
ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG CTGCGGACCC TACGTCCACT CAGGGTCATC 
AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA GAGACTCTGA TGTCATCCCT CAAACCCATT 
GGCAACATTG TGGTCATCTG CTGTGCCTTC TTCATCATTT TTGGAATTCT TGGGGTGCAG 
CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG GGTGAGGACA CCAGGAACAT CACTAACAAG 
TCCGACTGTG CTGAGGCCAG TTACCGGTGG GTCCGGCACA AGTACAACTT TGACAACCTG 
GGCCAGGCTC TGATGTCCCT GTTTGTGCTG GCCTCCAAGG ATGGCTGGGT TGACATCATG 
TATGATGGAC TGGATGCTGT GGGAGTGGAC CAGCAGCCCA TCATGAACCA CAACCCTTGG 
ATGCTGCTCT ACTTCATCTC CTTCCTCCTC ATCGTGGCCT TCTTCGTCCT GAACATGTTT 
GTGGGCGTGG TGGTGGAGAA CTTCCATAAG TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG 
CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG 
CAGATGGCCG ATCTAATGTT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG 
TCAGAAGCCC AGTGCAAACC CTACTACTCT GACTACTCGC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGACCTC TTCATCACTG GTGTCATCGG GCTGAATGTG 
GTCACGATGG CCATGGAACA TTACCAGCAG CCCCAGATCC TGGACGAGGC TCTGAAGATC 
TGCAACTACA TCTTTACCGT CATCTTTGTC TTGGAGTCAG TATTCAAACT TGTGGCCTTC 
GGCTTCCGCC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCTAT TGTGCTTCTG 
TCCATCATGG GCATCACGCT GGAAGAGATT GAGGTCAATG CTTCACTGCC CATCAACCCC 
ACCATCATCC GTATCATGAG GGTGCTCCGC ATTGCTCGAG TTCTGAAGCT GTTGAAGATG 
GCTGTGGGCA TGCGGGCACT GCTGGACACG GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
CTGGGACTTC TCTTCATGCT ATTATTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGACCTGG AGTGTGATGA GACACACCCT TGTGAGGGCT TGGGCCGGCA TGCCACCTTT 
AGGAACTTTG GTATGGCCTT TCTGACCCTC TTCCGAGTCT CCACTGGTGA CAACTGGAAT 
GGTATTATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACCGTC 
ATCTCACCCA TCTACTTCGT GTCCTTCGTG CTGACGGCCC AGTTTGTGCT GGTCAACGTG 
GTCATAGCCG TGCTGATGAA GCACCTGGAA GAGAGCAACA AAGAGGCCAA GGAGGAGGCG 
GAGTTGGAGG CGGAGCTGGA GCTAGAGATG AAGACACTCA GCCCGCAGCC CCACTCCCCG 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG 
CCTGGGGCTC CACACACCAC GGCCCACATT GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG 
CACCCCACGG ACAGACAGCT GTTTGACACC ATCTCCCTGC TGATCCAGGG CTCCCTGGAG 
GGGGAGCTGA AGCTGATGGA CGAGCTGGCA GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT 
TCCGCCCCCA GCCCGGGCGA CTCCGACCCA CAGATCCCTC TAGCTGAGAT GGAGGCTCTG 
TCTCTGACGT CAGAGATTGT GTCTGAACCG TCCTGCTCTC TAGCTCTGAC GGATGACTCT 
TTGCCTGATG ACACTCACAC ACTCTTACTT AGTGCCCTGG AGAGCAATAT GGTACCTCAC 
ACTGAGGAGG GGCCAGTCCC CCTAGGACCA GACCTGCTGA CTGTGAGGAA GTCTGGTGTC 
AGCCGGACAC ACTCTCTGCC CAATGACAGC TACATGTGCC GCAATGGGAG CACTGCCGAG 
AGATCCCTAG GACACAGGGG CTGGGGGCTC CCCAAAGCCC AGTCAGGCTC CATCTTGTCT 
GTTCACTCCC AACCAGCAGA CACCAGCTGC ATCCTACAGC TTCCCAAAGA TGCACACTAT 
CTGCTCCAGC CTCATGGGGC TCCCACCTGG GGCGCCATCC CTAAACTACC CCCACCTGGC 
CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG CGCCAGGCAG CAATAAGGAC TGACTCCCTG 
GACGTGCAGG GCCTGGGTAG CCGGGAAGAC CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC 
CCTCTGACCC GCTCCTCATC CTTCTGGGGC GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC 
GGCAGCCAGA GCAAAGTCTC CAAGCACATC CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA 
CCCAGCTGGG CCAAGGACCC TCAAGAGACC AGAAGCAGCT TAGAGCTGGA CACGGAGCTG 
AGCTGGATTT CAGGAGACCT CCTGCCCAGC AGTCAGGAAG AACCCCTGTC CCCACGGGAC 
TTGAAAAAAT GCTACAGTGT AGAGGCCCAG AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA 
GACGAACAGA GGAGACACTC CATCGCTGTC AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC 
CTATGTCCAA GCCCCTCAAG CCTCGGGGGC CAACCTCTTG GGGGCCCTGG GAGCCGGCCT 
AAGAAAAAAC TCAGCCCACC CAGTATCTCT ATAGACCCCC CGGAGAGCCA GGGCCCTCGG 
CCCCCATGCA GTCCTGGCGT CTGCCTCAGG AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT 
CCCTCGGCCT CCAGCCCCCT TGACAGCACG GCTGCCTCAC CCTCCCCAAA GAAAGATGCG 
CTGAGTCTCT CTGGTTTGTC TTCTGACCCA ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246990.1
CDS	773..7900
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248688.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248688.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
TTCTCTGAGA CCAAGCAACG GGAGAGTCAG CTGATGCGGG AGCAGCGTGT ACGATTCCTG 
TCCAATGCTA GCACCCTGGC AAGCTTCTCT GAGCCAGGCA GCTGCTATGA GGAGCTTCTC 
AAGTACCTGG TGTACATCCT CCGCAAAGCA GCCCGCAGGC TGGCCCAGGT CTCTAGGGCT 
GTAGGCGTGC GGGCTGGGTT GCTCAGCAGC CCAGTGGCCC GTGGTGGGCA GGAGCCCCAG 
CCCAGTGGCA GCTGCTCTCG TTCACACCGT CGTCTGTCTG TCCACCACCT GGTCCACCAC 
CATCACCACC ACCATCACCA CTACCACCTG GGTAATGGGA CGCTCAGAGT TCCCCGGGCC 
AGCCCAGAGA TCCAGGACAG GGATGCCAAT GGGTCCCGCT GGCTCATGCT GCCACCACCC 
TCTACCCCCA CTCCCTCTGG GGGCCCTCCG AGGGGTGCGG AGTCTGTACA CAGCTTCTAC 
CATGCTGACT GCCACTTGGA GCCAGTCCGT TGCCAGGCGC CCCCTCCCAG GTCCCCATCG 
GAGGCATCTG GCAGGACTGT GGGTAGTGGG AAGGTATACC CCACTGTGCA TACCAGCCCT 
CCACCAGAGA TGCTGAAGGA TAAGGCACTA GTGGAGGTGG CCCCCAGCCC TGGGCCCCCC 
ACCCTCACCA GCTTCAACAT CCCACCTGGG CCCTTCAGCT CCATGCACAA GCTCCTGGAG 
ACACAGAGTA CGGGTGAGAG CGTGGGAGCC TGCCATAGCT CCTGCAAAAT CTCCAGCCCT 
TGCTCCAAGG CAGACAGTGG AGCCTGTGGG CCGGACAGTT GTCCCTACTG TGCCCGGACA 
GGAGCAGGGG AGCCAGAGTC CGCTGACCAT GAAATGCCTG ACTCAGACAG TGAGGCTGTG 
TATGAGTTCA CACAGGACGC TCAGCACAGC GACCTCCGGG ATCCCCACAG ACGGCGACGG 
CCGAGCCTGG GCCCAGATGC AGAGCCTAGT TCTGTGCTGG CCTTCTGGAG GCTGATCTGC 
GACACATTCC GGAAGATCGT AGATAGCAAA TACTTTGGCC GGGGAATCAT GATCGCTATC 
CTGGTCAATA CCCTCAGCAT GGGCATCGAG TACCACGAGC AGCCCGAGGA GCTCACCAAC 
GCCCTGGAAA TCAGCAACAT CGTCTTCACC AGCCTCTTCG CCTTGGAGAT GCTGCTGAAG 
CTGCTCGTCT ACGGTCCCTT CGGCTACATT AAGAATCCCT ACAACATCTT TGATGGCGTC 
ATTGTGGTCA TCAGCGTGTG GGAGATTGTG GGCCAGCAGG GAGGTGGCCT GTCGGTGCTG 
CGGACCTTCC GCCTGATGCG GGTGCTGAAG CTAGTTCGCT TCCTGCCGGC ACTGCAGCGG 
CAGCTCGTGG TGCTCATGAA GACCATGGAC AACGTGGCCA CCTTCTGCAT GCTGCTCATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTTT TTGGTTGCAA GTTCGCATCT 
GAACGGGATG GGGACACGTT GCCAGACCGG AAGAATTTTG ACTCCCTGCT CTGGGCCATT 
GTCACTGTCT TTCAGATTCT GACTCAGGAA GACTGGAATA AAGTCCTTTA CAACGGCATG 
GCCTCCACGT CATCTTGGGC TGCTCTTTAC TTCATCGCCC TCATGACTTT TGGCAACTAC 
GTGCTCTTTA ACCTGCTTGT CGCCATTCTC GTGGAGGGTT TCCAGGCAGA GGAAATCGGC 
AAACGGGAAG ATACGAGTGG ACAGTTAAGC TGTATTCAGC TGCCTGTCAA CTCCCAGGGG 
GGAGATGCCA CCAAGTCTGA GTCAGAGCCT GATTTCTTTT CGCCCAGTGT GGATGGTGAT 
GGGGACAGGA AGAAGCGCTT GGCCCTGGTG GCCTTGGGAG AACACTCGGA ACTACGAAAG 
AGCCTTTTGC CACCTCTCAT CATCCACACA GCTGCTACAC CGATGTCACT GCCCAAGAGC 
TCCAGCACAG GTGTGGGGGA AGCACTGGGC TCTGGCTCTC GCCGCACCAG TAGCAGTGGG 
TCCGCTGAGC CTGGAACTGC CCATCATGAG ATGAAATCAC CGCCAAGTGC CCGAAGCTCC 
CCGCACAGTC CCTGGAGCGC AGCAAGCAGC TGGACCAGCA GGCGCTCCAG CCGGAACAGC 
CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG 
TCTGGAGAGG GTCAGGAGAG CCAGGATGAG GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC 
CCAGCAGGCA GTGATCATCG CCACAGGGGT TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT 
GACCTGCCTG ACACCCTGCA GGTGCCCGGG CTTCATCGAA CAGCCAGCGG TCGGAGCTCT 
GCCTCTGAAC ACCAAGACTG TAATGGCAAG TCGGCTTCAG GGCGTTTGGC CCGCACCCTG 
CGGGCTGATG ACCCCCCACT GGATGGGGAT GATGGCGATG ATGAGGGCAA CCTGAGCAAA 
GGGGAACGCT TACGAGCCTG GGTCCGAGCC CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT 
TCCTGGTCTG CCTATATCTT CCCTCCCCAG TCAAGGTTTC GTCTCCTGTG TCACCGGATC 
ATCACCCACA AGATGTTTGA CCATGTGGTC CTCGTCATCA TCTTCCTCAA CTGTATCACC 
ATCGCTATGG AGCGCCCCAA AATTGACCCC CACAGCGCTG AACGCATCTT CCTGACCCTC 
TCCAACTACA TCTTCACGGC AGTCTTCCTG GCTGAAATGA CAGTGAAGGT GGTGGCACTG 
GGCTGGTGCT TTGGGGAGCA GGCCTACCTG CGCAGCAGCT GGAACGTGCT GGACGGCTTG 
CTGGTGCTCA TCTCTGTCAT CGACATCCTG GTGTCCATGG TCTCTGACAG CGGCACCAAG 
ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG CTGCGGACCC TACGTCCACT CAGGGTCATC 
AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA GAGACTCTGA TGTCATCCCT CAAACCCATT 
GGCAACATTG TGGTCATCTG CTGTGCCTTC TTCATCATTT TTGGAATTCT TGGGGTGCAG 
CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG GGTGAGGACA CCAGGAACAT CACTAACAAG 
TCCGACTGTG CTGAGGCCAG TTACCGGTGG GTCCGGCACA AGTACAACTT TGACAACCTG 
GGCCAGGCTC TGATGTCCCT GTTTGTGCTG GCCTCCAAGG ATGGCTGGGT TGACATCATG 
TATGATGGAC TGGATGCTGT GGGAGTGGAC CAGCAGCCCA TCATGAACCA CAACCCTTGG 
ATGCTGCTCT ACTTCATCTC CTTCCTCCTC ATCGTGGCCT TCTTCGTCCT GAACATGTTT 
GTGGGCGTGG TGGTGGAGAA CTTCCATAAG TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG 
CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG 
CAGATGGCCG ATCTAATGTT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG 
TCAGAAGCCC AGTGCAAACC CTACTACTCT GACTACTCGC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGACCTC TTCATCACTG GTGTCATCGG GCTGAATGTG 
GTCACGATGG CCATGGAACA TTACCAGCAG CCCCAGATCC TGGACGAGGC TCTGAAGATC 
TGCAACTACA TCTTTACCGT CATCTTTGTC TTGGAGTCAG TATTCAAACT TGTGGCCTTC 
GGCTTCCGCC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCTAT TGTGCTTCTG 
TCCATCATGG GCATCACGCT GGAAGAGATT GAGGTCAATG CTTCACTGCC CATCAACCCC 
ACCATCATCC GTATCATGAG GGTGCTCCGC ATTGCTCGAG TTCTGAAGCT GTTGAAGATG 
GCTGTGGGCA TGCGGGCACT GCTGGACACG GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
CTGGGACTTC TCTTCATGCT ATTATTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGACCTGG AGTGTGATGA GACACACCCT TGTGAGGGCT TGGGCCGGCA TGCCACCTTT 
AGGAACTTTG GTATGGCCTT TCTGACCCTC TTCCGAGTCT CCACTGGTGA CAACTGGAAT 
GGTATTATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACCGTC 
ATCTCACCCA TCTACTTCGT GTCCTTCGTG CTGACGGCCC AGTTTGTGCT GGTCAACGTG 
GTCATAGCCG TGCTGATGAA GCACCTGGAA GAGAGCAACA AAGAGGCCAA GGAGGAGGCG 
GAGTTGGAGG CGGAGCTGGA GCTAGAGATG AAGACACTCA GCCCGCAGCC CCACTCCCCG 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG 
CCTGGGGCTC CACACACCAC GGCCCACATT GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG 
CACCCCACGG ACAGACAGCT GTTTGACACC ATCTCCCTGC TGATCCAGGG CTCCCTGGAG 
GGGGAGCTGA AGCTGATGGA CGAGCTGGCA GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT 
TCCGCCCCCA GCCCGGGCGA CTCCGACCCA CAGATCCCTC TAGCTGAGAT GGAGGCTCTG 
TCTCTGACGT CAGAGATTGT GTCTGAACCG TCCTGCTCTC TAGCTCTGAC GGATGACTCT 
TTGCCTGATG ACACTCACAC ACTCTTACTT AGTGCCCTGG AGAGCAATAT GGTACCTCAC 
ACTGAGGAGG GGCCAGTCCC CCTAGGACCA GACCTGCTGA CTGTGAGGAA GTCTGGTGTC 
AGCCGGACAC ACTCTCTGCC CAATGACAGC TACATGTGCC GCAATGGGAG CACTGCCGAG 
AGATCCCTAG GACACAGGGG CTGGGGGCTC CCCAAAGCCC AGTCAGGCTC CATCTTGTCT 
GTTCACTCCC AACCAGCAGA CACCAGCTGC ATCCTACAGC TTCCCAAAGA TGCACACTAT 
CTGCTCCAGC CTCATGGGGC TCCCACCTGG GGCGCCATCC CTAAACTACC CCCACCTGGC 
CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG CGCCAGGCAG CAATAAGGAC TGACTCCCTG 
GACGTGCAGG GCCTGGGTAG CCGGGAAGAC CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC 
CCTCTGACCC GCTCCTCATC CTTCTGGGGC GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC 
GGCAGCCAGA GCAAAGTCTC CAAGCACATC CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA 
CCCAGCTGGG CCAAGGACCC TCAAGAGACC AGAAGCAGCT TAGAGCTGGA CACGGAGCTG 
AGCTGGATTT CAGGAGACCT CCTGCCCAGC AGTCAGGAAG AACCCCTGTC CCCACGGGAC 
TTGAAAAAAT GCTACAGTGT AGAGGCCCAG AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA 
GACGAACAGA GGAGACACTC CATCGCTGTC AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC 
CTATGTCCAA GCCCCTCAAG CCTCGGGGGC CAACCTCTTG GGGGCCCTGG GAGCCGGCCT 
AAGAAAAAAC TCAGCCCACC CAGTATCTCT ATAGACCCCC CGGAGAGCCA GGGCCCTCGG 
CCCCCATGCA GTCCTGGCGT CTGCCTCAGG AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT 
CCCTCGGCCT CCAGCCCCCT TGACAGCACG GCTGCCTCAC CCTCCCCAAA GAAAGATGCG 
CTGAGTCTCT CTGGTTTGTC TTCTGACCCA ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248689.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246991.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X6, mRNA.

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CloneID	OMu67271
Clone ID Related Accession (Same CDS sequence)	XM_011248689.2 , XM_011248689.3
Accession Version	XM_011248689.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248689.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GAAGCCCAGT GCAAACCCTA CTACTCTGAC TACTCGCGCT TCCGGCTCCT CGTCCACCAC 
CTGTGTACCA GCCACTACCT GGACCTCTTC ATCACTGGTG TCATCGGGCT GAATGTGGTC 
ACGATGGCCA TGGAACATTA CCAGCAGCCC CAGATCCTGG ACGAGGCTCT GAAGATCTGC 
AACTACATCT TTACCGTCAT CTTTGTCTTG GAGTCAGTAT TCAAACTTGT GGCCTTCGGC 
TTCCGCCGGT TCTTCCAGGA CAGGTGGAAC CAGCTGGACC TGGCTATTGT GCTTCTGTCC 
ATCATGGGCA TCACGCTGGA AGAGATTGAG GTCAATGCTT CACTGCCCAT CAACCCCACC 
ATCATCCGTA TCATGAGGGT GCTCCGCATT GCTCGAGTTC TGAAGCTGTT GAAGATGGCT 
GTGGGCATGC GGGCACTGCT GGACACGGTG ATGCAGGCCC TGCCCCAGGT GGGGAACCTG 
GGACTTCTCT TCATGCTATT ATTTTTCATC TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA 
GACCTGGAGT GTGATGAGAC ACACCCTTGT GAGGGCTTGG GCCGGCATGC CACCTTTAGG 
AACTTTGGTA TGGCCTTTCT GACCCTCTTC CGAGTCTCCA CTGGTGACAA CTGGAATGGT 
ATTATGAAGG ACACCCTCCG GGACTGTGAC CAGGAGTCCA CCTGCTACAA CACCGTCATC 
TCACCCATCT ACTTCGTGTC CTTCGTGCTG ACGGCCCAGT TTGTGCTGGT CAACGTGGTC 
ATAGCCGTGC TGATGAAGCA CCTGGAAGAG AGCAACAAAG AGGCCAAGGA GGAGGCGGAG 
TTGGAGGCGG AGCTGGAGCT AGAGATGAAG ACACTCAGCC CGCAGCCCCA CTCCCCGCTG 
GGCAGCCCCT TCCTCTGGCC TGGGGTGGAA GGTGTCAATA GCCCTGACAG CCCTAAGCCT 
GGGGCTCCAC ACACCACGGC CCACATTGGA GCAGCCTCTT CAGGCTTCTC CCTTGAGCAC 
CCCACGGACA GACAGCTGTT TGACACCATC TCCCTGCTGA TCCAGGGCTC CCTGGAGGGG 
GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTTCC 
GCCCCCAGCC CGGGCGACTC CGACCCACAG ATCCCTCTAG CTGAGATGGA GGCTCTGTCT 
CTGACGTCAG AGATTGTGTC TGAACCGTCC TGCTCTCTAG CTCTGACGGA TGACTCTTTG 
CCTGATGACA CTCACACACT CTTACTTAGT GCCCTGGAGA GCAATATGGT ACCTCACACT 
GAGGAGGGGC CAGTCCCCCT AGGACCAGAC CTGCTGACTG TGAGGAAGTC TGGTGTCAGC 
CGGACACACT CTCTGCCCAA TGACAGCTAC ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA 
TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT 
CACTCCCAAC CAGCAGACAC CAGCTGCATC CTACAGCTTC CCAAAGATGC ACACTATCTG 
CTCCAGCCTC ATGGGGCTCC CACCTGGGGC GCCATCCCTA AACTACCCCC ACCTGGCCGC 
TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC CAGGCAGCAA TAAGGACTGA CTCCCTGGAC 
GTGCAGGGCC TGGGTAGCCG GGAAGACCTG TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT 
CTGACCCGCT CCTCATCCTT CTGGGGCGGG TCGAGCATCC AGGTGCAGCA GCGCTCCGGC 
AGCCAGAGCA AAGTCTCCAA GCACATCCGC CTGCCAGCCC CTTGCCCAGG CCTGGAACCC 
AGCTGGGCCA AGGACCCTCA AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCAGCAGT CAGGAAGAAC CCCTGTCCCC ACGGGACTTG 
AAAAAATGCT ACAGTGTAGA GGCCCAGAGC TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC 
GAACAGAGGA GACACTCCAT CGCTGTCAGC TGCCTGGACA GCGGCTCCCA GCCCCGCCTA 
TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG 
AAAAAACTCA GCCCACCCAG TATCTCTATA GACCCCCCGG AGAGCCAGGG CCCTCGGCCC 
CCATGCAGTC CTGGCGTCTG CCTCAGGAGG AGGGCGCCGG CCAGTGACTC GAAGGATCCC 
TCGGCCTCCA GCCCCCTTGA CAGCACGGCT GCCTCACCCT CCCCAAAGAA AGATGCGCTG 
AGTCTCTCTG GTTTGTCTTC TGACCCAACA GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246991.1
CDS	784..7908
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248689.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248689.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GAAGCCCAGT GCAAACCCTA CTACTCTGAC TACTCGCGCT TCCGGCTCCT CGTCCACCAC 
CTGTGTACCA GCCACTACCT GGACCTCTTC ATCACTGGTG TCATCGGGCT GAATGTGGTC 
ACGATGGCCA TGGAACATTA CCAGCAGCCC CAGATCCTGG ACGAGGCTCT GAAGATCTGC 
AACTACATCT TTACCGTCAT CTTTGTCTTG GAGTCAGTAT TCAAACTTGT GGCCTTCGGC 
TTCCGCCGGT TCTTCCAGGA CAGGTGGAAC CAGCTGGACC TGGCTATTGT GCTTCTGTCC 
ATCATGGGCA TCACGCTGGA AGAGATTGAG GTCAATGCTT CACTGCCCAT CAACCCCACC 
ATCATCCGTA TCATGAGGGT GCTCCGCATT GCTCGAGTTC TGAAGCTGTT GAAGATGGCT 
GTGGGCATGC GGGCACTGCT GGACACGGTG ATGCAGGCCC TGCCCCAGGT GGGGAACCTG 
GGACTTCTCT TCATGCTATT ATTTTTCATC TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA 
GACCTGGAGT GTGATGAGAC ACACCCTTGT GAGGGCTTGG GCCGGCATGC CACCTTTAGG 
AACTTTGGTA TGGCCTTTCT GACCCTCTTC CGAGTCTCCA CTGGTGACAA CTGGAATGGT 
ATTATGAAGG ACACCCTCCG GGACTGTGAC CAGGAGTCCA CCTGCTACAA CACCGTCATC 
TCACCCATCT ACTTCGTGTC CTTCGTGCTG ACGGCCCAGT TTGTGCTGGT CAACGTGGTC 
ATAGCCGTGC TGATGAAGCA CCTGGAAGAG AGCAACAAAG AGGCCAAGGA GGAGGCGGAG 
TTGGAGGCGG AGCTGGAGCT AGAGATGAAG ACACTCAGCC CGCAGCCCCA CTCCCCGCTG 
GGCAGCCCCT TCCTCTGGCC TGGGGTGGAA GGTGTCAATA GCCCTGACAG CCCTAAGCCT 
GGGGCTCCAC ACACCACGGC CCACATTGGA GCAGCCTCTT CAGGCTTCTC CCTTGAGCAC 
CCCACGGACA GACAGCTGTT TGACACCATC TCCCTGCTGA TCCAGGGCTC CCTGGAGGGG 
GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTTCC 
GCCCCCAGCC CGGGCGACTC CGACCCACAG ATCCCTCTAG CTGAGATGGA GGCTCTGTCT 
CTGACGTCAG AGATTGTGTC TGAACCGTCC TGCTCTCTAG CTCTGACGGA TGACTCTTTG 
CCTGATGACA CTCACACACT CTTACTTAGT GCCCTGGAGA GCAATATGGT ACCTCACACT 
GAGGAGGGGC CAGTCCCCCT AGGACCAGAC CTGCTGACTG TGAGGAAGTC TGGTGTCAGC 
CGGACACACT CTCTGCCCAA TGACAGCTAC ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA 
TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT 
CACTCCCAAC CAGCAGACAC CAGCTGCATC CTACAGCTTC CCAAAGATGC ACACTATCTG 
CTCCAGCCTC ATGGGGCTCC CACCTGGGGC GCCATCCCTA AACTACCCCC ACCTGGCCGC 
TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC CAGGCAGCAA TAAGGACTGA CTCCCTGGAC 
GTGCAGGGCC TGGGTAGCCG GGAAGACCTG TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT 
CTGACCCGCT CCTCATCCTT CTGGGGCGGG TCGAGCATCC AGGTGCAGCA GCGCTCCGGC 
AGCCAGAGCA AAGTCTCCAA GCACATCCGC CTGCCAGCCC CTTGCCCAGG CCTGGAACCC 
AGCTGGGCCA AGGACCCTCA AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCAGCAGT CAGGAAGAAC CCCTGTCCCC ACGGGACTTG 
AAAAAATGCT ACAGTGTAGA GGCCCAGAGC TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC 
GAACAGAGGA GACACTCCAT CGCTGTCAGC TGCCTGGACA GCGGCTCCCA GCCCCGCCTA 
TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG 
AAAAAACTCA GCCCACCCAG TATCTCTATA GACCCCCCGG AGAGCCAGGG CCCTCGGCCC 
CCATGCAGTC CTGGCGTCTG CCTCAGGAGG AGGGCGCCGG CCAGTGACTC GAAGGATCCC 
TCGGCCTCCA GCCCCCTTGA CAGCACGGCT GCCTCACCCT CCCCAAAGAA AGATGCGCTG 
AGTCTCTCTG GTTTGTCTTC TGACCCAACA GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248690.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246992.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X8, mRNA.

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CloneID	OMu67272
Clone ID Related Accession (Same CDS sequence)	XM_011248690.2 , XM_011248690.3
Accession Version	XM_011248690.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248690.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGGAGATGC CACCAAGTCT 
GAGTCAGAGC CTGATTTCTT TTCGCCCAGT GTGGATGGTG ATGGGGACAG GAAGAAGCGC 
TTGGCCCTGG TGGCCTTGGG AGAACACTCG GAACTACGAA AGAGCCTTTT GCCACCTCTC 
ATCATCCACA CAGCTGCTAC ACCGATGTCA CTGCCCAAGA GCTCCAGCAC AGGTGTGGGG 
GAAGCACTGG GCTCTGGCTC TCGCCGCACC AGTAGCAGTG GGTCCGCTGA GCCTGGAACT 
GCCCATCATG AGATGAAATC ACCGCCAAGT GCCCGAAGCT CCCCGCACAG TCCCTGGAGC 
GCAGCAAGCA GCTGGACCAG CAGGCGCTCC AGCCGGAACA GCCTGGGCCG GGCCCCCAGC 
CTGAAGCGTA GGAGCCCAAG CGGGGAGCGG AGGTCCCTGC TGTCTGGAGA GGGTCAGGAG 
AGCCAGGATG AGGAGGAGAG TTCAGAAGAG GACCGGGCCA GCCCAGCAGG CAGTGATCAT 
CGCCACAGGG GTTCCTTGGA ACGTGAGGCC AAGAGTTCCT TTGACCTGCC TGACACCCTG 
CAGGTGCCCG GGCTTCATCG AACAGCCAGC GGTCGGAGCT CTGCCTCTGA ACACCAAGAC 
TGTAATGGCA AGTCGGCTTC AGGGCGTTTG GCCCGCACCC TGCGGGCTGA TGACCCCCCA 
CTGGATGGGG ATGATGGCGA TGATGAGGGC AACCTGAGCA AAGGGGAACG CTTACGAGCC 
TGGGTCCGAG CCCGGCTCCC TGCCTGTTGC CGAGAGCGAG ATTCCTGGTC TGCCTATATC 
TTCCCTCCCC AGTCAAGGTT TCGTCTCCTG TGTCACCGGA TCATCACCCA CAAGATGTTT 
GACCATGTGG TCCTCGTCAT CATCTTCCTC AACTGTATCA CCATCGCTAT GGAGCGCCCC 
AAAATTGACC CCCACAGCGC TGAACGCATC TTCCTGACCC TCTCCAACTA CATCTTCACG 
GCAGTCTTCC TGGCTGAAAT GACAGTGAAG GTGGTGGCAC TGGGCTGGTG CTTTGGGGAG 
CAGGCCTACC TGCGCAGCAG CTGGAACGTG CTGGACGGCT TGCTGGTGCT CATCTCTGTC 
ATCGACATCC TGGTGTCCAT GGTCTCTGAC AGCGGCACCA AGATTCTCGG CATGCTGAGG 
GTGCTGCGGC TGCTGCGGAC CCTACGTCCA CTCAGGGTCA TCAGCCGGGC CCAGGGGCTG 
AAGCTGGTGG TAGAGACTCT GATGTCATCC CTCAAACCCA TTGGCAACAT TGTGGTCATC 
TGCTGTGCCT TCTTCATCAT TTTTGGAATT CTTGGGGTGC AGCTCTTCAA AGGGAAGTTC 
TTCGTGTGTC AGGGTGAGGA CACCAGGAAC ATCACTAACA AGTCCGACTG TGCTGAGGCC 
AGTTACCGGT GGGTCCGGCA CAAGTACAAC TTTGACAACC TGGGCCAGGC TCTGATGTCC 
CTGTTTGTGC TGGCCTCCAA GGATGGCTGG GTTGACATCA TGTATGATGG ACTGGATGCT 
GTGGGAGTGG ACCAGCAGCC CATCATGAAC CACAACCCTT GGATGCTGCT CTACTTCATC 
TCCTTCCTCC TCATCGTGGC CTTCTTCGTC CTGAACATGT TTGTGGGCGT GGTGGTGGAG 
AACTTCCATA AGTGCAGGCA GCACCAGGAG GAGGAGGAGG CGCGGCGGCG GGAGGAGAAG 
CGACTAAAGA GGCTGGAGAA AAAGAGAAGG AGTAAGGAGA AGCAGATGGC CGATCTAATG 
TTGGACGATG TAATTGCTTC CGGCAGCTCA GCCAGCGCTG CGTCAGAAGC CCAGTGCAAA 
CCCTACTACT CTGACTACTC GCGCTTCCGG CTCCTCGTCC ACCACCTGTG TACCAGCCAC 
TACCTGGACC TCTTCATCAC TGGTGTCATC GGGCTGAATG TGGTCACGAT GGCCATGGAA 
CATTACCAGC AGCCCCAGAT CCTGGACGAG GCTCTGAAGA TCTGCAACTA CATCTTTACC 
GTCATCTTTG TCTTGGAGTC AGTATTCAAA CTTGTGGCCT TCGGCTTCCG CCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCT ATTGTGCTTC TGTCCATCAT GGGCATCACG 
CTGGAAGAGA TTGAGGTCAA TGCTTCACTG CCCATCAACC CCACCATCAT CCGTATCATG 
AGGGTGCTCC GCATTGCTCG AGTTCTGAAG CTGTTGAAGA TGGCTGTGGG CATGCGGGCA 
CTGCTGGACA CGGTGATGCA GGCCCTGCCC CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
CTATTATTTT TCATCTTTGC AGCTCTGGGC GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACACACC CTTGTGAGGG CTTGGGCCGG CATGCCACCT TTAGGAACTT TGGTATGGCC 
TTTCTGACCC TCTTCCGAGT CTCCACTGGT GACAACTGGA ATGGTATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACCTGC TACAACACCG TCATCTCACC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTCATAGC CGTGCTGATG 
AAGCACCTGG AAGAGAGCAA CAAAGAGGCC AAGGAGGAGG CGGAGTTGGA GGCGGAGCTG 
GAGCTAGAGA TGAAGACACT CAGCCCGCAG CCCCACTCCC CGCTGGGCAG CCCCTTCCTC 
TGGCCTGGGG TGGAAGGTGT CAATAGCCCT GACAGCCCTA AGCCTGGGGC TCCACACACC 
ACGGCCCACA TTGGAGCAGC CTCTTCAGGC TTCTCCCTTG AGCACCCCAC GGACAGACAG 
CTGTTTGACA CCATCTCCCT GCTGATCCAG GGCTCCCTGG AGGGGGAGCT GAAGCTGATG 
GACGAGCTGG CAGGCCCAGG GGGCCAGCCC TCTGCCTTCC CTTCCGCCCC CAGCCCGGGC 
GACTCCGACC CACAGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTCTGAC GTCAGAGATT 
GTGTCTGAAC CGTCCTGCTC TCTAGCTCTG ACGGATGACT CTTTGCCTGA TGACACTCAC 
ACACTCTTAC TTAGTGCCCT GGAGAGCAAT ATGGTACCTC ACACTGAGGA GGGGCCAGTC 
CCCCTAGGAC CAGACCTGCT GACTGTGAGG AAGTCTGGTG TCAGCCGGAC ACACTCTCTG 
CCCAATGACA GCTACATGTG CCGCAATGGG AGCACTGCCG AGAGATCCCT AGGACACAGG 
GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC TCCATCTTGT CTGTTCACTC CCAACCAGCA 
GACACCAGCT GCATCCTACA GCTTCCCAAA GATGCACACT ATCTGCTCCA GCCTCATGGG 
GCTCCCACCT GGGGCGCCAT CCCTAAACTA CCCCCACCTG GCCGCTCCCC TCTGGCTCAG 
AGGCCTCTCA GGCGCCAGGC AGCAATAAGG ACTGACTCCC TGGACGTGCA GGGCCTGGGT 
AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA 
TCCTTCTGGG GCGGGTCGAG CATCCAGGTG CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC 
TCCAAGCACA TCCGCCTGCC AGCCCCTTGC CCAGGCCTGG AACCCAGCTG GGCCAAGGAC 
CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGCCCA GCAGTCAGGA AGAACCCCTG TCCCCACGGG ACTTGAAAAA ATGCTACAGT 
GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT GGGTCCTGGC TAGACGAACA GAGGAGACAC 
TCCATCGCTG TCAGCTGCCT GGACAGCGGC TCCCAGCCCC GCCTATGTCC AAGCCCCTCA 
AGCCTCGGGG GCCAACCTCT TGGGGGCCCT GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA 
CCCAGTATCT CTATAGACCC CCCGGAGAGC CAGGGCCCTC GGCCCCCATG CAGTCCTGGC 
GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT GACTCGAAGG ATCCCTCGGC CTCCAGCCCC 
CTTGACAGCA CGGCTGCCTC ACCCTCCCCA AAGAAAGATG CGCTGAGTCT CTCTGGTTTG 
TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246992.1
CDS	784..7893
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248690.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248690.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGGAGATGC CACCAAGTCT 
GAGTCAGAGC CTGATTTCTT TTCGCCCAGT GTGGATGGTG ATGGGGACAG GAAGAAGCGC 
TTGGCCCTGG TGGCCTTGGG AGAACACTCG GAACTACGAA AGAGCCTTTT GCCACCTCTC 
ATCATCCACA CAGCTGCTAC ACCGATGTCA CTGCCCAAGA GCTCCAGCAC AGGTGTGGGG 
GAAGCACTGG GCTCTGGCTC TCGCCGCACC AGTAGCAGTG GGTCCGCTGA GCCTGGAACT 
GCCCATCATG AGATGAAATC ACCGCCAAGT GCCCGAAGCT CCCCGCACAG TCCCTGGAGC 
GCAGCAAGCA GCTGGACCAG CAGGCGCTCC AGCCGGAACA GCCTGGGCCG GGCCCCCAGC 
CTGAAGCGTA GGAGCCCAAG CGGGGAGCGG AGGTCCCTGC TGTCTGGAGA GGGTCAGGAG 
AGCCAGGATG AGGAGGAGAG TTCAGAAGAG GACCGGGCCA GCCCAGCAGG CAGTGATCAT 
CGCCACAGGG GTTCCTTGGA ACGTGAGGCC AAGAGTTCCT TTGACCTGCC TGACACCCTG 
CAGGTGCCCG GGCTTCATCG AACAGCCAGC GGTCGGAGCT CTGCCTCTGA ACACCAAGAC 
TGTAATGGCA AGTCGGCTTC AGGGCGTTTG GCCCGCACCC TGCGGGCTGA TGACCCCCCA 
CTGGATGGGG ATGATGGCGA TGATGAGGGC AACCTGAGCA AAGGGGAACG CTTACGAGCC 
TGGGTCCGAG CCCGGCTCCC TGCCTGTTGC CGAGAGCGAG ATTCCTGGTC TGCCTATATC 
TTCCCTCCCC AGTCAAGGTT TCGTCTCCTG TGTCACCGGA TCATCACCCA CAAGATGTTT 
GACCATGTGG TCCTCGTCAT CATCTTCCTC AACTGTATCA CCATCGCTAT GGAGCGCCCC 
AAAATTGACC CCCACAGCGC TGAACGCATC TTCCTGACCC TCTCCAACTA CATCTTCACG 
GCAGTCTTCC TGGCTGAAAT GACAGTGAAG GTGGTGGCAC TGGGCTGGTG CTTTGGGGAG 
CAGGCCTACC TGCGCAGCAG CTGGAACGTG CTGGACGGCT TGCTGGTGCT CATCTCTGTC 
ATCGACATCC TGGTGTCCAT GGTCTCTGAC AGCGGCACCA AGATTCTCGG CATGCTGAGG 
GTGCTGCGGC TGCTGCGGAC CCTACGTCCA CTCAGGGTCA TCAGCCGGGC CCAGGGGCTG 
AAGCTGGTGG TAGAGACTCT GATGTCATCC CTCAAACCCA TTGGCAACAT TGTGGTCATC 
TGCTGTGCCT TCTTCATCAT TTTTGGAATT CTTGGGGTGC AGCTCTTCAA AGGGAAGTTC 
TTCGTGTGTC AGGGTGAGGA CACCAGGAAC ATCACTAACA AGTCCGACTG TGCTGAGGCC 
AGTTACCGGT GGGTCCGGCA CAAGTACAAC TTTGACAACC TGGGCCAGGC TCTGATGTCC 
CTGTTTGTGC TGGCCTCCAA GGATGGCTGG GTTGACATCA TGTATGATGG ACTGGATGCT 
GTGGGAGTGG ACCAGCAGCC CATCATGAAC CACAACCCTT GGATGCTGCT CTACTTCATC 
TCCTTCCTCC TCATCGTGGC CTTCTTCGTC CTGAACATGT TTGTGGGCGT GGTGGTGGAG 
AACTTCCATA AGTGCAGGCA GCACCAGGAG GAGGAGGAGG CGCGGCGGCG GGAGGAGAAG 
CGACTAAAGA GGCTGGAGAA AAAGAGAAGG AGTAAGGAGA AGCAGATGGC CGATCTAATG 
TTGGACGATG TAATTGCTTC CGGCAGCTCA GCCAGCGCTG CGTCAGAAGC CCAGTGCAAA 
CCCTACTACT CTGACTACTC GCGCTTCCGG CTCCTCGTCC ACCACCTGTG TACCAGCCAC 
TACCTGGACC TCTTCATCAC TGGTGTCATC GGGCTGAATG TGGTCACGAT GGCCATGGAA 
CATTACCAGC AGCCCCAGAT CCTGGACGAG GCTCTGAAGA TCTGCAACTA CATCTTTACC 
GTCATCTTTG TCTTGGAGTC AGTATTCAAA CTTGTGGCCT TCGGCTTCCG CCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCT ATTGTGCTTC TGTCCATCAT GGGCATCACG 
CTGGAAGAGA TTGAGGTCAA TGCTTCACTG CCCATCAACC CCACCATCAT CCGTATCATG 
AGGGTGCTCC GCATTGCTCG AGTTCTGAAG CTGTTGAAGA TGGCTGTGGG CATGCGGGCA 
CTGCTGGACA CGGTGATGCA GGCCCTGCCC CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
CTATTATTTT TCATCTTTGC AGCTCTGGGC GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACACACC CTTGTGAGGG CTTGGGCCGG CATGCCACCT TTAGGAACTT TGGTATGGCC 
TTTCTGACCC TCTTCCGAGT CTCCACTGGT GACAACTGGA ATGGTATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACCTGC TACAACACCG TCATCTCACC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTCATAGC CGTGCTGATG 
AAGCACCTGG AAGAGAGCAA CAAAGAGGCC AAGGAGGAGG CGGAGTTGGA GGCGGAGCTG 
GAGCTAGAGA TGAAGACACT CAGCCCGCAG CCCCACTCCC CGCTGGGCAG CCCCTTCCTC 
TGGCCTGGGG TGGAAGGTGT CAATAGCCCT GACAGCCCTA AGCCTGGGGC TCCACACACC 
ACGGCCCACA TTGGAGCAGC CTCTTCAGGC TTCTCCCTTG AGCACCCCAC GGACAGACAG 
CTGTTTGACA CCATCTCCCT GCTGATCCAG GGCTCCCTGG AGGGGGAGCT GAAGCTGATG 
GACGAGCTGG CAGGCCCAGG GGGCCAGCCC TCTGCCTTCC CTTCCGCCCC CAGCCCGGGC 
GACTCCGACC CACAGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTCTGAC GTCAGAGATT 
GTGTCTGAAC CGTCCTGCTC TCTAGCTCTG ACGGATGACT CTTTGCCTGA TGACACTCAC 
ACACTCTTAC TTAGTGCCCT GGAGAGCAAT ATGGTACCTC ACACTGAGGA GGGGCCAGTC 
CCCCTAGGAC CAGACCTGCT GACTGTGAGG AAGTCTGGTG TCAGCCGGAC ACACTCTCTG 
CCCAATGACA GCTACATGTG CCGCAATGGG AGCACTGCCG AGAGATCCCT AGGACACAGG 
GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC TCCATCTTGT CTGTTCACTC CCAACCAGCA 
GACACCAGCT GCATCCTACA GCTTCCCAAA GATGCACACT ATCTGCTCCA GCCTCATGGG 
GCTCCCACCT GGGGCGCCAT CCCTAAACTA CCCCCACCTG GCCGCTCCCC TCTGGCTCAG 
AGGCCTCTCA GGCGCCAGGC AGCAATAAGG ACTGACTCCC TGGACGTGCA GGGCCTGGGT 
AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA 
TCCTTCTGGG GCGGGTCGAG CATCCAGGTG CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC 
TCCAAGCACA TCCGCCTGCC AGCCCCTTGC CCAGGCCTGG AACCCAGCTG GGCCAAGGAC 
CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGCCCA GCAGTCAGGA AGAACCCCTG TCCCCACGGG ACTTGAAAAA ATGCTACAGT 
GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT GGGTCCTGGC TAGACGAACA GAGGAGACAC 
TCCATCGCTG TCAGCTGCCT GGACAGCGGC TCCCAGCCCC GCCTATGTCC AAGCCCCTCA 
AGCCTCGGGG GCCAACCTCT TGGGGGCCCT GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA 
CCCAGTATCT CTATAGACCC CCCGGAGAGC CAGGGCCCTC GGCCCCCATG CAGTCCTGGC 
GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT GACTCGAAGG ATCCCTCGGC CTCCAGCCCC 
CTTGACAGCA CGGCTGCCTC ACCCTCCCCA AAGAAAGATG CGCTGAGTCT CTCTGGTTTG 
TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248691.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246993.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X9, mRNA.

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CloneID	OMu67273
Clone ID Related Accession (Same CDS sequence)	XM_011248691.3 , XM_011248691.2
Accession Version	XM_011248691.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248691.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGGACAG ACAGCTGTTT 
GACACCATCT CCCTGCTGAT CCAGGGCTCC CTGGAGGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTTCCG CCCCCAGCCC GGGCGACTCC 
GACCCACAGA TCCCTCTAGC TGAGATGGAG GCTCTGTCTC TGACGTCAGA GATTGTGTCT 
GAACCGTCCT GCTCTCTAGC TCTGACGGAT GACTCTTTGC CTGATGACAC TCACACACTC 
TTACTTAGTG CCCTGGAGAG CAATATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246993.1
CDS	784..7887
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248691.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248691.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGGACAG ACAGCTGTTT 
GACACCATCT CCCTGCTGAT CCAGGGCTCC CTGGAGGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTTCCG CCCCCAGCCC GGGCGACTCC 
GACCCACAGA TCCCTCTAGC TGAGATGGAG GCTCTGTCTC TGACGTCAGA GATTGTGTCT 
GAACCGTCCT GCTCTCTAGC TCTGACGGAT GACTCTTTGC CTGATGACAC TCACACACTC 
TTACTTAGTG CCCTGGAGAG CAATATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248692.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246994.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X14, mRNA.

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CloneID	OMu67274
Clone ID Related Accession (Same CDS sequence)	XM_011248692.3 , XM_011248692.2
Accession Version	XM_011248692.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248692.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
GACAGACAGC TGTTTGACAC CATCTCCCTG CTGATCCAGG GCTCCCTGGA GGGGGAGCTG 
AAGCTGATGG ACGAGCTGGC AGGCCCAGGG GGCCAGCCCT CTGCCTTCCC TTCCGCCCCC 
AGCCCGGGCG ACTCCGACCC ACAGATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246994.1
CDS	784..7827
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248692.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248692.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
GACAGACAGC TGTTTGACAC CATCTCCCTG CTGATCCAGG GCTCCCTGGA GGGGGAGCTG 
AAGCTGATGG ACGAGCTGGC AGGCCCAGGG GGCCAGCCCT CTGCCTTCCC TTCCGCCCCC 
AGCCCGGGCG ACTCCGACCC ACAGATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248693.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246995.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X15, mRNA.

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CloneID	OMu67275
Clone ID Related Accession (Same CDS sequence)	XM_011248693.2 , XM_011248693.3
Accession Version	XM_011248693.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248693.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
ATCCCTCTAG CTGAGATGGA GGCTCTGTCT CTGACGTCAG AGATTGTGTC TGAACCGTCC 
TGCTCTCTAG CTCTGACGGA TGACTCTTTG CCTGATGACA CTCACACACT CTTACTTAGT 
GCCCTGGAGA GCAATATGGT ACCTCACACT GAGGAGGGGC CAGTCCCCCT AGGACCAGAC 
CTGCTGACTG TGAGGAAGTC TGGTGTCAGC CGGACACACT CTCTGCCCAA TGACAGCTAC 
ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC 
AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT CACTCCCAAC CAGCAGACAC CAGCTGCATC 
CTACAGCTTC CCAAAGATGC ACACTATCTG CTCCAGCCTC ATGGGGCTCC CACCTGGGGC 
GCCATCCCTA AACTACCCCC ACCTGGCCGC TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC 
CAGGCAGCAA TAAGGACTGA CTCCCTGGAC GTGCAGGGCC TGGGTAGCCG GGAAGACCTG 
TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT CTGACCCGCT CCTCATCCTT CTGGGGCGGG 
TCGAGCATCC AGGTGCAGCA GCGCTCCGGC AGCCAGAGCA AAGTCTCCAA GCACATCCGC 
CTGCCAGCCC CTTGCCCAGG CCTGGAACCC AGCTGGGCCA AGGACCCTCA AGAGACCAGA 
AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GCCCAGCAGT 
CAGGAAGAAC CCCTGTCCCC ACGGGACTTG AAAAAATGCT ACAGTGTAGA GGCCCAGAGC 
TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC GAACAGAGGA GACACTCCAT CGCTGTCAGC 
TGCCTGGACA GCGGCTCCCA GCCCCGCCTA TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA 
CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG AAAAAACTCA GCCCACCCAG TATCTCTATA 
GACCCCCCGG AGAGCCAGGG CCCTCGGCCC CCATGCAGTC CTGGCGTCTG CCTCAGGAGG 
AGGGCGCCGG CCAGTGACTC GAAGGATCCC TCGGCCTCCA GCCCCCTTGA CAGCACGGCT 
GCCTCACCCT CCCCAAAGAA AGATGCGCTG AGTCTCTCTG GTTTGTCTTC TGACCCAACA 
GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246995.1
CDS	784..7818
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248693.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248693.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
ATCCCTCTAG CTGAGATGGA GGCTCTGTCT CTGACGTCAG AGATTGTGTC TGAACCGTCC 
TGCTCTCTAG CTCTGACGGA TGACTCTTTG CCTGATGACA CTCACACACT CTTACTTAGT 
GCCCTGGAGA GCAATATGGT ACCTCACACT GAGGAGGGGC CAGTCCCCCT AGGACCAGAC 
CTGCTGACTG TGAGGAAGTC TGGTGTCAGC CGGACACACT CTCTGCCCAA TGACAGCTAC 
ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC 
AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT CACTCCCAAC CAGCAGACAC CAGCTGCATC 
CTACAGCTTC CCAAAGATGC ACACTATCTG CTCCAGCCTC ATGGGGCTCC CACCTGGGGC 
GCCATCCCTA AACTACCCCC ACCTGGCCGC TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC 
CAGGCAGCAA TAAGGACTGA CTCCCTGGAC GTGCAGGGCC TGGGTAGCCG GGAAGACCTG 
TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT CTGACCCGCT CCTCATCCTT CTGGGGCGGG 
TCGAGCATCC AGGTGCAGCA GCGCTCCGGC AGCCAGAGCA AAGTCTCCAA GCACATCCGC 
CTGCCAGCCC CTTGCCCAGG CCTGGAACCC AGCTGGGCCA AGGACCCTCA AGAGACCAGA 
AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GCCCAGCAGT 
CAGGAAGAAC CCCTGTCCCC ACGGGACTTG AAAAAATGCT ACAGTGTAGA GGCCCAGAGC 
TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC GAACAGAGGA GACACTCCAT CGCTGTCAGC 
TGCCTGGACA GCGGCTCCCA GCCCCGCCTA TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA 
CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG AAAAAACTCA GCCCACCCAG TATCTCTATA 
GACCCCCCGG AGAGCCAGGG CCCTCGGCCC CCATGCAGTC CTGGCGTCTG CCTCAGGAGG 
AGGGCGCCGG CCAGTGACTC GAAGGATCCC TCGGCCTCCA GCCCCCTTGA CAGCACGGCT 
GCCTCACCCT CCCCAAAGAA AGATGCGCTG AGTCTCTCTG GTTTGTCTTC TGACCCAACA 
GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248694.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246996.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X17, mRNA.

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CloneID	OMu67276
Clone ID Related Accession (Same CDS sequence)	XM_011248694.2 , XM_011248694.3
Accession Version	XM_011248694.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248694.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GAAGCCCAGT GCAAACCCTA CTACTCTGAC TACTCGCGCT TCCGGCTCCT CGTCCACCAC 
CTGTGTACCA GCCACTACCT GGACCTCTTC ATCACTGGTG TCATCGGGCT GAATGTGGTC 
ACGATGGCCA TGGAACATTA CCAGCAGCCC CAGATCCTGG ACGAGGCTCT GAAGATCTGC 
AACTACATCT TTACCGTCAT CTTTGTCTTG GAGTCAGTAT TCAAACTTGT GGCCTTCGGC 
TTCCGCCGGT TCTTCCAGGA CAGGTGGAAC CAGCTGGACC TGGCTATTGT GCTTCTGTCC 
ATCATGGGCA TCACGCTGGA AGAGATTGAG GTCAATGCTT CACTGCCCAT CAACCCCACC 
ATCATCCGTA TCATGAGGGT GCTCCGCATT GCTCGAGTTC TGAAGCTGTT GAAGATGGCT 
GTGGGCATGC GGGCACTGCT GGACACGGTG ATGCAGGCCC TGCCCCAGGT GGGGAACCTG 
GGACTTCTCT TCATGCTATT ATTTTTCATC TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA 
GACCTGGAGT GTGATGAGAC ACACCCTTGT GAGGGCTTGG GCCGGCATGC CACCTTTAGG 
AACTTTGGTA TGGCCTTTCT GACCCTCTTC CGAGTCTCCA CTGGTGACAA CTGGAATGGT 
ATTATGAAGG ACACCCTCCG GGACTGTGAC CAGGAGTCCA CCTGCTACAA CACCGTCATC 
TCACCCATCT ACTTCGTGTC CTTCGTGCTG ACGGCCCAGT TTGTGCTGGT CAACGTGGTC 
ATAGCCGTGC TGATGAAGCA CCTGGAAGAG AGCAACAAAG AGGCCAAGGA GGAGGCGGAG 
TTGGAGGCGG AGCTGGAGCT AGAGATGAAG ACACTCAGCC CGCAGCCCCA CTCCCCGCTG 
GGCAGCCCCT TCCTCTGGCC TGGGGTGGAA GGTGTCAATA GCCCTGACAG CCCTAAGCCT 
GGGGCTCCAC ACACCACGGC CCACATTGGA GCAGCCTCTT CAGGCTTCTC CCTTGAGCAC 
CCCACGGACA GACAGCTGTT TGACACCATC TCCCTGCTGA TCCAGGGCTC CCTGGAGGGG 
GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTTCC 
GCCCCCAGCC CGGGCGACTC CGACCCACAG ATGGTACCTC ACACTGAGGA GGGGCCAGTC 
CCCCTAGGAC CAGACCTGCT GACTGTGAGG AAGTCTGGTG TCAGCCGGAC ACACTCTCTG 
CCCAATGACA GCTACATGTG CCGCAATGGG AGCACTGCCG AGAGATCCCT AGGACACAGG 
GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC TCCATCTTGT CTGTTCACTC CCAACCAGCA 
GACACCAGCT GCATCCTACA GCTTCCCAAA GATGCACACT ATCTGCTCCA GCCTCATGGG 
GCTCCCACCT GGGGCGCCAT CCCTAAACTA CCCCCACCTG GCCGCTCCCC TCTGGCTCAG 
AGGCCTCTCA GGCGCCAGGC AGCAATAAGG ACTGACTCCC TGGACGTGCA GGGCCTGGGT 
AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA 
TCCTTCTGGG GCGGGTCGAG CATCCAGGTG CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC 
TCCAAGCACA TCCGCCTGCC AGCCCCTTGC CCAGGCCTGG AACCCAGCTG GGCCAAGGAC 
CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGCCCA GCAGTCAGGA AGAACCCCTG TCCCCACGGG ACTTGAAAAA ATGCTACAGT 
GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT GGGTCCTGGC TAGACGAACA GAGGAGACAC 
TCCATCGCTG TCAGCTGCCT GGACAGCGGC TCCCAGCCCC GCCTATGTCC AAGCCCCTCA 
AGCCTCGGGG GCCAACCTCT TGGGGGCCCT GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA 
CCCAGTATCT CTATAGACCC CCCGGAGAGC CAGGGCCCTC GGCCCCCATG CAGTCCTGGC 
GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT GACTCGAAGG ATCCCTCGGC CTCCAGCCCC 
CTTGACAGCA CGGCTGCCTC ACCCTCCCCA AAGAAAGATG CGCTGAGTCT CTCTGGTTTG 
TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246996.1
CDS	784..7773
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248694.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248694.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GAAGCCCAGT GCAAACCCTA CTACTCTGAC TACTCGCGCT TCCGGCTCCT CGTCCACCAC 
CTGTGTACCA GCCACTACCT GGACCTCTTC ATCACTGGTG TCATCGGGCT GAATGTGGTC 
ACGATGGCCA TGGAACATTA CCAGCAGCCC CAGATCCTGG ACGAGGCTCT GAAGATCTGC 
AACTACATCT TTACCGTCAT CTTTGTCTTG GAGTCAGTAT TCAAACTTGT GGCCTTCGGC 
TTCCGCCGGT TCTTCCAGGA CAGGTGGAAC CAGCTGGACC TGGCTATTGT GCTTCTGTCC 
ATCATGGGCA TCACGCTGGA AGAGATTGAG GTCAATGCTT CACTGCCCAT CAACCCCACC 
ATCATCCGTA TCATGAGGGT GCTCCGCATT GCTCGAGTTC TGAAGCTGTT GAAGATGGCT 
GTGGGCATGC GGGCACTGCT GGACACGGTG ATGCAGGCCC TGCCCCAGGT GGGGAACCTG 
GGACTTCTCT TCATGCTATT ATTTTTCATC TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA 
GACCTGGAGT GTGATGAGAC ACACCCTTGT GAGGGCTTGG GCCGGCATGC CACCTTTAGG 
AACTTTGGTA TGGCCTTTCT GACCCTCTTC CGAGTCTCCA CTGGTGACAA CTGGAATGGT 
ATTATGAAGG ACACCCTCCG GGACTGTGAC CAGGAGTCCA CCTGCTACAA CACCGTCATC 
TCACCCATCT ACTTCGTGTC CTTCGTGCTG ACGGCCCAGT TTGTGCTGGT CAACGTGGTC 
ATAGCCGTGC TGATGAAGCA CCTGGAAGAG AGCAACAAAG AGGCCAAGGA GGAGGCGGAG 
TTGGAGGCGG AGCTGGAGCT AGAGATGAAG ACACTCAGCC CGCAGCCCCA CTCCCCGCTG 
GGCAGCCCCT TCCTCTGGCC TGGGGTGGAA GGTGTCAATA GCCCTGACAG CCCTAAGCCT 
GGGGCTCCAC ACACCACGGC CCACATTGGA GCAGCCTCTT CAGGCTTCTC CCTTGAGCAC 
CCCACGGACA GACAGCTGTT TGACACCATC TCCCTGCTGA TCCAGGGCTC CCTGGAGGGG 
GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTTCC 
GCCCCCAGCC CGGGCGACTC CGACCCACAG ATGGTACCTC ACACTGAGGA GGGGCCAGTC 
CCCCTAGGAC CAGACCTGCT GACTGTGAGG AAGTCTGGTG TCAGCCGGAC ACACTCTCTG 
CCCAATGACA GCTACATGTG CCGCAATGGG AGCACTGCCG AGAGATCCCT AGGACACAGG 
GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC TCCATCTTGT CTGTTCACTC CCAACCAGCA 
GACACCAGCT GCATCCTACA GCTTCCCAAA GATGCACACT ATCTGCTCCA GCCTCATGGG 
GCTCCCACCT GGGGCGCCAT CCCTAAACTA CCCCCACCTG GCCGCTCCCC TCTGGCTCAG 
AGGCCTCTCA GGCGCCAGGC AGCAATAAGG ACTGACTCCC TGGACGTGCA GGGCCTGGGT 
AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA 
TCCTTCTGGG GCGGGTCGAG CATCCAGGTG CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC 
TCCAAGCACA TCCGCCTGCC AGCCCCTTGC CCAGGCCTGG AACCCAGCTG GGCCAAGGAC 
CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGCCCA GCAGTCAGGA AGAACCCCTG TCCCCACGGG ACTTGAAAAA ATGCTACAGT 
GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT GGGTCCTGGC TAGACGAACA GAGGAGACAC 
TCCATCGCTG TCAGCTGCCT GGACAGCGGC TCCCAGCCCC GCCTATGTCC AAGCCCCTCA 
AGCCTCGGGG GCCAACCTCT TGGGGGCCCT GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA 
CCCAGTATCT CTATAGACCC CCCGGAGAGC CAGGGCCCTC GGCCCCCATG CAGTCCTGGC 
GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT GACTCGAAGG ATCCCTCGGC CTCCAGCCCC 
CTTGACAGCA CGGCTGCCTC ACCCTCCCCA AAGAAAGATG CGCTGAGTCT CTCTGGTTTG 
TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248695.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246997.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X23, mRNA.

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CloneID	OMu67277
Clone ID Related Accession (Same CDS sequence)	XM_011248695.2 , XM_011248695.3
Accession Version	XM_011248695.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248695.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
ATGGTACCTC ACACTGAGGA GGGGCCAGTC CCCCTAGGAC CAGACCTGCT GACTGTGAGG 
AAGTCTGGTG TCAGCCGGAC ACACTCTCTG CCCAATGACA GCTACATGTG CCGCAATGGG 
AGCACTGCCG AGAGATCCCT AGGACACAGG GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC 
TCCATCTTGT CTGTTCACTC CCAACCAGCA GACACCAGCT GCATCCTACA GCTTCCCAAA 
GATGCACACT ATCTGCTCCA GCCTCATGGG GCTCCCACCT GGGGCGCCAT CCCTAAACTA 
CCCCCACCTG GCCGCTCCCC TCTGGCTCAG AGGCCTCTCA GGCGCCAGGC AGCAATAAGG 
ACTGACTCCC TGGACGTGCA GGGCCTGGGT AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT 
GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA TCCTTCTGGG GCGGGTCGAG CATCCAGGTG 
CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC TCCAAGCACA TCCGCCTGCC AGCCCCTTGC 
CCAGGCCTGG AACCCAGCTG GGCCAAGGAC CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA GCAGTCAGGA AGAACCCCTG 
TCCCCACGGG ACTTGAAAAA ATGCTACAGT GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT 
GGGTCCTGGC TAGACGAACA GAGGAGACAC TCCATCGCTG TCAGCTGCCT GGACAGCGGC 
TCCCAGCCCC GCCTATGTCC AAGCCCCTCA AGCCTCGGGG GCCAACCTCT TGGGGGCCCT 
GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA CCCAGTATCT CTATAGACCC CCCGGAGAGC 
CAGGGCCCTC GGCCCCCATG CAGTCCTGGC GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT 
GACTCGAAGG ATCCCTCGGC CTCCAGCCCC CTTGACAGCA CGGCTGCCTC ACCCTCCCCA 
AAGAAAGATG CGCTGAGTCT CTCTGGTTTG TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246997.1
CDS	784..7683
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248695.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248695.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
ATGGTACCTC ACACTGAGGA GGGGCCAGTC CCCCTAGGAC CAGACCTGCT GACTGTGAGG 
AAGTCTGGTG TCAGCCGGAC ACACTCTCTG CCCAATGACA GCTACATGTG CCGCAATGGG 
AGCACTGCCG AGAGATCCCT AGGACACAGG GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC 
TCCATCTTGT CTGTTCACTC CCAACCAGCA GACACCAGCT GCATCCTACA GCTTCCCAAA 
GATGCACACT ATCTGCTCCA GCCTCATGGG GCTCCCACCT GGGGCGCCAT CCCTAAACTA 
CCCCCACCTG GCCGCTCCCC TCTGGCTCAG AGGCCTCTCA GGCGCCAGGC AGCAATAAGG 
ACTGACTCCC TGGACGTGCA GGGCCTGGGT AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT 
GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA TCCTTCTGGG GCGGGTCGAG CATCCAGGTG 
CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC TCCAAGCACA TCCGCCTGCC AGCCCCTTGC 
CCAGGCCTGG AACCCAGCTG GGCCAAGGAC CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA GCAGTCAGGA AGAACCCCTG 
TCCCCACGGG ACTTGAAAAA ATGCTACAGT GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT 
GGGTCCTGGC TAGACGAACA GAGGAGACAC TCCATCGCTG TCAGCTGCCT GGACAGCGGC 
TCCCAGCCCC GCCTATGTCC AAGCCCCTCA AGCCTCGGGG GCCAACCTCT TGGGGGCCCT 
GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA CCCAGTATCT CTATAGACCC CCCGGAGAGC 
CAGGGCCCTC GGCCCCCATG CAGTCCTGGC GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT 
GACTCGAAGG ATCCCTCGGC CTCCAGCCCC CTTGACAGCA CGGCTGCCTC ACCCTCCCCA 
AAGAAAGATG CGCTGAGTCT CTCTGGTTTG TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248696.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246998.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X24, mRNA.

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CloneID	OMu67278
Clone ID Related Accession (Same CDS sequence)	XM_011248696.2 , XM_011248696.3
Accession Version	XM_011248696.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248696.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA ATCTAATGTT GGACGATGTA 
ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG TCAGAAGCCC AGTGCAAACC CTACTACTCT 
GACTACTCGC GCTTCCGGCT CCTCGTCCAC CACCTGTGTA CCAGCCACTA CCTGGACCTC 
TTCATCACTG GTGTCATCGG GCTGAATGTG GTCACGATGG CCATGGAACA TTACCAGCAG 
CCCCAGATCC TGGACGAGGC TCTGAAGATC TGCAACTACA TCTTTACCGT CATCTTTGTC 
TTGGAGTCAG TATTCAAACT TGTGGCCTTC GGCTTCCGCC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCTAT TGTGCTTCTG TCCATCATGG GCATCACGCT GGAAGAGATT 
GAGGTCAATG CTTCACTGCC CATCAACCCC ACCATCATCC GTATCATGAG GGTGCTCCGC 
ATTGCTCGAG TTCTGAAGCT GTTGAAGATG GCTGTGGGCA TGCGGGCACT GCTGGACACG 
GTGATGCAGG CCCTGCCCCA GGTGGGGAAC CTGGGACTTC TCTTCATGCT ATTATTTTTC 
ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACACACCCT 
TGTGAGGGCT TGGGCCGGCA TGCCACCTTT AGGAACTTTG GTATGGCCTT TCTGACCCTC 
TTCCGAGTCT CCACTGGTGA CAACTGGAAT GGTATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACCTGCTA CAACACCGTC ATCTCACCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTCATAGCCG TGCTGATGAA GCACCTGGAA 
GAGAGCAACA AAGAGGCCAA GGAGGAGGCG GAGTTGGAGG CGGAGCTGGA GCTAGAGATG 
AAGACACTCA GCCCGCAGCC CCACTCCCCG CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG 
GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG CCTGGGGCTC CACACACCAC GGCCCACATT 
GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG CACCCCACGA TGGTACCTCA CACTGAGGAG 
GGGCCAGTCC CCCTAGGACC AGACCTGCTG ACTGTGAGGA AGTCTGGTGT CAGCCGGACA 
CACTCTCTGC CCAATGACAG CTACATGTGC CGCAATGGGA GCACTGCCGA GAGATCCCTA 
GGACACAGGG GCTGGGGGCT CCCCAAAGCC CAGTCAGGCT CCATCTTGTC TGTTCACTCC 
CAACCAGCAG ACACCAGCTG CATCCTACAG CTTCCCAAAG ATGCACACTA TCTGCTCCAG 
CCTCATGGGG CTCCCACCTG GGGCGCCATC CCTAAACTAC CCCCACCTGG CCGCTCCCCT 
CTGGCTCAGA GGCCTCTCAG GCGCCAGGCA GCAATAAGGA CTGACTCCCT GGACGTGCAG 
GGCCTGGGTA GCCGGGAAGA CCTGTTGTCA GAGGTGAGTG GGCCCTCCTG CCCTCTGACC 
CGCTCCTCAT CCTTCTGGGG CGGGTCGAGC ATCCAGGTGC AGCAGCGCTC CGGCAGCCAG 
AGCAAAGTCT CCAAGCACAT CCGCCTGCCA GCCCCTTGCC CAGGCCTGGA ACCCAGCTGG 
GCCAAGGACC CTCAAGAGAC CAGAAGCAGC TTAGAGCTGG ACACGGAGCT GAGCTGGATT 
TCAGGAGACC TCCTGCCCAG CAGTCAGGAA GAACCCCTGT CCCCACGGGA CTTGAAAAAA 
TGCTACAGTG TAGAGGCCCA GAGCTGCCGG CGCAGGCCTG GGTCCTGGCT AGACGAACAG 
AGGAGACACT CCATCGCTGT CAGCTGCCTG GACAGCGGCT CCCAGCCCCG CCTATGTCCA 
AGCCCCTCAA GCCTCGGGGG CCAACCTCTT GGGGGCCCTG GGAGCCGGCC TAAGAAAAAA 
CTCAGCCCAC CCAGTATCTC TATAGACCCC CCGGAGAGCC AGGGCCCTCG GCCCCCATGC 
AGTCCTGGCG TCTGCCTCAG GAGGAGGGCG CCGGCCAGTG ACTCGAAGGA TCCCTCGGCC 
TCCAGCCCCC TTGACAGCAC GGCTGCCTCA CCCTCCCCAA AGAAAGATGC GCTGAGTCTC 
TCTGGTTTGT CTTCTGACCC AACAGACCTG GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246998.1
CDS	784..7662
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248696.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248696.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA ATCTAATGTT GGACGATGTA 
ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG TCAGAAGCCC AGTGCAAACC CTACTACTCT 
GACTACTCGC GCTTCCGGCT CCTCGTCCAC CACCTGTGTA CCAGCCACTA CCTGGACCTC 
TTCATCACTG GTGTCATCGG GCTGAATGTG GTCACGATGG CCATGGAACA TTACCAGCAG 
CCCCAGATCC TGGACGAGGC TCTGAAGATC TGCAACTACA TCTTTACCGT CATCTTTGTC 
TTGGAGTCAG TATTCAAACT TGTGGCCTTC GGCTTCCGCC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCTAT TGTGCTTCTG TCCATCATGG GCATCACGCT GGAAGAGATT 
GAGGTCAATG CTTCACTGCC CATCAACCCC ACCATCATCC GTATCATGAG GGTGCTCCGC 
ATTGCTCGAG TTCTGAAGCT GTTGAAGATG GCTGTGGGCA TGCGGGCACT GCTGGACACG 
GTGATGCAGG CCCTGCCCCA GGTGGGGAAC CTGGGACTTC TCTTCATGCT ATTATTTTTC 
ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACACACCCT 
TGTGAGGGCT TGGGCCGGCA TGCCACCTTT AGGAACTTTG GTATGGCCTT TCTGACCCTC 
TTCCGAGTCT CCACTGGTGA CAACTGGAAT GGTATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACCTGCTA CAACACCGTC ATCTCACCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTCATAGCCG TGCTGATGAA GCACCTGGAA 
GAGAGCAACA AAGAGGCCAA GGAGGAGGCG GAGTTGGAGG CGGAGCTGGA GCTAGAGATG 
AAGACACTCA GCCCGCAGCC CCACTCCCCG CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG 
GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG CCTGGGGCTC CACACACCAC GGCCCACATT 
GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG CACCCCACGA TGGTACCTCA CACTGAGGAG 
GGGCCAGTCC CCCTAGGACC AGACCTGCTG ACTGTGAGGA AGTCTGGTGT CAGCCGGACA 
CACTCTCTGC CCAATGACAG CTACATGTGC CGCAATGGGA GCACTGCCGA GAGATCCCTA 
GGACACAGGG GCTGGGGGCT CCCCAAAGCC CAGTCAGGCT CCATCTTGTC TGTTCACTCC 
CAACCAGCAG ACACCAGCTG CATCCTACAG CTTCCCAAAG ATGCACACTA TCTGCTCCAG 
CCTCATGGGG CTCCCACCTG GGGCGCCATC CCTAAACTAC CCCCACCTGG CCGCTCCCCT 
CTGGCTCAGA GGCCTCTCAG GCGCCAGGCA GCAATAAGGA CTGACTCCCT GGACGTGCAG 
GGCCTGGGTA GCCGGGAAGA CCTGTTGTCA GAGGTGAGTG GGCCCTCCTG CCCTCTGACC 
CGCTCCTCAT CCTTCTGGGG CGGGTCGAGC ATCCAGGTGC AGCAGCGCTC CGGCAGCCAG 
AGCAAAGTCT CCAAGCACAT CCGCCTGCCA GCCCCTTGCC CAGGCCTGGA ACCCAGCTGG 
GCCAAGGACC CTCAAGAGAC CAGAAGCAGC TTAGAGCTGG ACACGGAGCT GAGCTGGATT 
TCAGGAGACC TCCTGCCCAG CAGTCAGGAA GAACCCCTGT CCCCACGGGA CTTGAAAAAA 
TGCTACAGTG TAGAGGCCCA GAGCTGCCGG CGCAGGCCTG GGTCCTGGCT AGACGAACAG 
AGGAGACACT CCATCGCTGT CAGCTGCCTG GACAGCGGCT CCCAGCCCCG CCTATGTCCA 
AGCCCCTCAA GCCTCGGGGG CCAACCTCTT GGGGGCCCTG GGAGCCGGCC TAAGAAAAAA 
CTCAGCCCAC CCAGTATCTC TATAGACCCC CCGGAGAGCC AGGGCCCTCG GCCCCCATGC 
AGTCCTGGCG TCTGCCTCAG GAGGAGGGCG CCGGCCAGTG ACTCGAAGGA TCCCTCGGCC 
TCCAGCCCCC TTGACAGCAC GGCTGCCTCA CCCTCCCCAA AGAAAGATGC GCTGAGTCTC 
TCTGGTTTGT CTTCTGACCC AACAGACCTG GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248697.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246999.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X26, mRNA.

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CloneID	OMu67279
Clone ID Related Accession (Same CDS sequence)	XM_011248697.3 , XM_011248697.2
Accession Version	XM_011248697.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248697.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GAAGCCCAGT GCAAACCCTA CTACTCTGAC TACTCGCGCT TCCGGCTCCT CGTCCACCAC 
CTGTGTACCA GCCACTACCT GGACCTCTTC ATCACTGGTG TCATCGGGCT GAATGTGGTC 
ACGATGGCCA TGGAACATTA CCAGCAGCCC CAGATCCTGG ACGAGGCTCT GAAGATCTGC 
AACTACATCT TTACCGTCAT CTTTGTCTTG GAGTCAGTAT TCAAACTTGT GGCCTTCGGC 
TTCCGCCGGT TCTTCCAGGA CAGGTGGAAC CAGCTGGACC TGGCTATTGT GCTTCTGTCC 
ATCATGGGCA TCACGCTGGA AGAGATTGAG GTCAATGCTT CACTGCCCAT CAACCCCACC 
ATCATCCGTA TCATGAGGGT GCTCCGCATT GCTCGAGTTC TGAAGCTGTT GAAGATGGCT 
GTGGGCATGC GGGCACTGCT GGACACGGTG ATGCAGGCCC TGCCCCAGGT GGGGAACCTG 
GGACTTCTCT TCATGCTATT ATTTTTCATC TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA 
GACCTGGAGT GTGATGAGAC ACACCCTTGT GAGGGCTTGG GCCGGCATGC CACCTTTAGG 
AACTTTGGTA TGGCCTTTCT GACCCTCTTC CGAGTCTCCA CTGGTGACAA CTGGAATGGT 
ATTATGAAGG ACACCCTCCG GGACTGTGAC CAGGAGTCCA CCTGCTACAA CACCGTCATC 
TCACCCATCT ACTTCGTGTC CTTCGTGCTG ACGGCCCAGT TTGTGCTGGT CAACGTGGTC 
ATAGCCGTGC TGATGAAGCA CCTGGAAGAG AGCAACAAAG AGGCCAAGGA GGAGGCGGAG 
TTGGAGGCGG AGCTGGAGCT AGAGATGAAG ACACTCAGCC CGCAGCCCCA CTCCCCGCTG 
GGCAGCCCCT TCCTCTGGCC TGGGGTGGAA GGTGTCAATA GCCCTGACAG CCCTAAGCCT 
GGGGCTCCAC ACACCACGGC CCACATTGGA GCAGCCTCTT CAGGCTTCTC CCTTGAGCAC 
CCCACGATGG TACCTCACAC TGAGGAGGGG CCAGTCCCCC TAGGACCAGA CCTGCTGACT 
GTGAGGAAGT CTGGTGTCAG CCGGACACAC TCTCTGCCCA ATGACAGCTA CATGTGCCGC 
AATGGGAGCA CTGCCGAGAG ATCCCTAGGA CACAGGGGCT GGGGGCTCCC CAAAGCCCAG 
TCAGGCTCCA TCTTGTCTGT TCACTCCCAA CCAGCAGACA CCAGCTGCAT CCTACAGCTT 
CCCAAAGATG CACACTATCT GCTCCAGCCT CATGGGGCTC CCACCTGGGG CGCCATCCCT 
AAACTACCCC CACCTGGCCG CTCCCCTCTG GCTCAGAGGC CTCTCAGGCG CCAGGCAGCA 
ATAAGGACTG ACTCCCTGGA CGTGCAGGGC CTGGGTAGCC GGGAAGACCT GTTGTCAGAG 
GTGAGTGGGC CCTCCTGCCC TCTGACCCGC TCCTCATCCT TCTGGGGCGG GTCGAGCATC 
CAGGTGCAGC AGCGCTCCGG CAGCCAGAGC AAAGTCTCCA AGCACATCCG CCTGCCAGCC 
CCTTGCCCAG GCCTGGAACC CAGCTGGGCC AAGGACCCTC AAGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCAGCAG TCAGGAAGAA 
CCCCTGTCCC CACGGGACTT GAAAAAATGC TACAGTGTAG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGGGT CCTGGCTAGA CGAACAGAGG AGACACTCCA TCGCTGTCAG CTGCCTGGAC 
AGCGGCTCCC AGCCCCGCCT ATGTCCAAGC CCCTCAAGCC TCGGGGGCCA ACCTCTTGGG 
GGCCCTGGGA GCCGGCCTAA GAAAAAACTC AGCCCACCCA GTATCTCTAT AGACCCCCCG 
GAGAGCCAGG GCCCTCGGCC CCCATGCAGT CCTGGCGTCT GCCTCAGGAG GAGGGCGCCG 
GCCAGTGACT CGAAGGATCC CTCGGCCTCC AGCCCCCTTG ACAGCACGGC TGCCTCACCC 
TCCCCAAAGA AAGATGCGCT GAGTCTCTCT GGTTTGTCTT CTGACCCAAC AGACCTGGAT 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246999.1
CDS	784..7629
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248697.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248697.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GAAGCCCAGT GCAAACCCTA CTACTCTGAC TACTCGCGCT TCCGGCTCCT CGTCCACCAC 
CTGTGTACCA GCCACTACCT GGACCTCTTC ATCACTGGTG TCATCGGGCT GAATGTGGTC 
ACGATGGCCA TGGAACATTA CCAGCAGCCC CAGATCCTGG ACGAGGCTCT GAAGATCTGC 
AACTACATCT TTACCGTCAT CTTTGTCTTG GAGTCAGTAT TCAAACTTGT GGCCTTCGGC 
TTCCGCCGGT TCTTCCAGGA CAGGTGGAAC CAGCTGGACC TGGCTATTGT GCTTCTGTCC 
ATCATGGGCA TCACGCTGGA AGAGATTGAG GTCAATGCTT CACTGCCCAT CAACCCCACC 
ATCATCCGTA TCATGAGGGT GCTCCGCATT GCTCGAGTTC TGAAGCTGTT GAAGATGGCT 
GTGGGCATGC GGGCACTGCT GGACACGGTG ATGCAGGCCC TGCCCCAGGT GGGGAACCTG 
GGACTTCTCT TCATGCTATT ATTTTTCATC TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA 
GACCTGGAGT GTGATGAGAC ACACCCTTGT GAGGGCTTGG GCCGGCATGC CACCTTTAGG 
AACTTTGGTA TGGCCTTTCT GACCCTCTTC CGAGTCTCCA CTGGTGACAA CTGGAATGGT 
ATTATGAAGG ACACCCTCCG GGACTGTGAC CAGGAGTCCA CCTGCTACAA CACCGTCATC 
TCACCCATCT ACTTCGTGTC CTTCGTGCTG ACGGCCCAGT TTGTGCTGGT CAACGTGGTC 
ATAGCCGTGC TGATGAAGCA CCTGGAAGAG AGCAACAAAG AGGCCAAGGA GGAGGCGGAG 
TTGGAGGCGG AGCTGGAGCT AGAGATGAAG ACACTCAGCC CGCAGCCCCA CTCCCCGCTG 
GGCAGCCCCT TCCTCTGGCC TGGGGTGGAA GGTGTCAATA GCCCTGACAG CCCTAAGCCT 
GGGGCTCCAC ACACCACGGC CCACATTGGA GCAGCCTCTT CAGGCTTCTC CCTTGAGCAC 
CCCACGATGG TACCTCACAC TGAGGAGGGG CCAGTCCCCC TAGGACCAGA CCTGCTGACT 
GTGAGGAAGT CTGGTGTCAG CCGGACACAC TCTCTGCCCA ATGACAGCTA CATGTGCCGC 
AATGGGAGCA CTGCCGAGAG ATCCCTAGGA CACAGGGGCT GGGGGCTCCC CAAAGCCCAG 
TCAGGCTCCA TCTTGTCTGT TCACTCCCAA CCAGCAGACA CCAGCTGCAT CCTACAGCTT 
CCCAAAGATG CACACTATCT GCTCCAGCCT CATGGGGCTC CCACCTGGGG CGCCATCCCT 
AAACTACCCC CACCTGGCCG CTCCCCTCTG GCTCAGAGGC CTCTCAGGCG CCAGGCAGCA 
ATAAGGACTG ACTCCCTGGA CGTGCAGGGC CTGGGTAGCC GGGAAGACCT GTTGTCAGAG 
GTGAGTGGGC CCTCCTGCCC TCTGACCCGC TCCTCATCCT TCTGGGGCGG GTCGAGCATC 
CAGGTGCAGC AGCGCTCCGG CAGCCAGAGC AAAGTCTCCA AGCACATCCG CCTGCCAGCC 
CCTTGCCCAG GCCTGGAACC CAGCTGGGCC AAGGACCCTC AAGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCAGCAG TCAGGAAGAA 
CCCCTGTCCC CACGGGACTT GAAAAAATGC TACAGTGTAG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGGGT CCTGGCTAGA CGAACAGAGG AGACACTCCA TCGCTGTCAG CTGCCTGGAC 
AGCGGCTCCC AGCCCCGCCT ATGTCCAAGC CCCTCAAGCC TCGGGGGCCA ACCTCTTGGG 
GGCCCTGGGA GCCGGCCTAA GAAAAAACTC AGCCCACCCA GTATCTCTAT AGACCCCCCG 
GAGAGCCAGG GCCCTCGGCC CCCATGCAGT CCTGGCGTCT GCCTCAGGAG GAGGGCGCCG 
GCCAGTGACT CGAAGGATCC CTCGGCCTCC AGCCCCCTTG ACAGCACGGC TGCCTCACCC 
TCCCCAAAGA AAGATGCGCT GAGTCTCTCT GGTTTGTCTT CTGACCCAAC AGACCTGGAT 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248698.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247000.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X28, mRNA.

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CloneID	OMu67280
Clone ID Related Accession (Same CDS sequence)	XM_011248698.3 , XM_011248698.2
Accession Version	XM_011248698.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGATGGT ACCTCACACT 
GAGGAGGGGC CAGTCCCCCT AGGACCAGAC CTGCTGACTG TGAGGAAGTC TGGTGTCAGC 
CGGACACACT CTCTGCCCAA TGACAGCTAC ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA 
TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT 
CACTCCCAAC CAGCAGACAC CAGCTGCATC CTACAGCTTC CCAAAGATGC ACACTATCTG 
CTCCAGCCTC ATGGGGCTCC CACCTGGGGC GCCATCCCTA AACTACCCCC ACCTGGCCGC 
TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC CAGGCAGCAA TAAGGACTGA CTCCCTGGAC 
GTGCAGGGCC TGGGTAGCCG GGAAGACCTG TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT 
CTGACCCGCT CCTCATCCTT CTGGGGCGGG TCGAGCATCC AGGTGCAGCA GCGCTCCGGC 
AGCCAGAGCA AAGTCTCCAA GCACATCCGC CTGCCAGCCC CTTGCCCAGG CCTGGAACCC 
AGCTGGGCCA AGGACCCTCA AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCAGCAGT CAGGAAGAAC CCCTGTCCCC ACGGGACTTG 
AAAAAATGCT ACAGTGTAGA GGCCCAGAGC TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC 
GAACAGAGGA GACACTCCAT CGCTGTCAGC TGCCTGGACA GCGGCTCCCA GCCCCGCCTA 
TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG 
AAAAAACTCA GCCCACCCAG TATCTCTATA GACCCCCCGG AGAGCCAGGG CCCTCGGCCC 
CCATGCAGTC CTGGCGTCTG CCTCAGGAGG AGGGCGCCGG CCAGTGACTC GAAGGATCCC 
TCGGCCTCCA GCCCCCTTGA CAGCACGGCT GCCTCACCCT CCCCAAAGAA AGATGCGCTG 
AGTCTCTCTG GTTTGTCTTC TGACCCAACA GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247000.1
CDS	784..7608
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248698.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248698.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGATGGT ACCTCACACT 
GAGGAGGGGC CAGTCCCCCT AGGACCAGAC CTGCTGACTG TGAGGAAGTC TGGTGTCAGC 
CGGACACACT CTCTGCCCAA TGACAGCTAC ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA 
TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT 
CACTCCCAAC CAGCAGACAC CAGCTGCATC CTACAGCTTC CCAAAGATGC ACACTATCTG 
CTCCAGCCTC ATGGGGCTCC CACCTGGGGC GCCATCCCTA AACTACCCCC ACCTGGCCGC 
TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC CAGGCAGCAA TAAGGACTGA CTCCCTGGAC 
GTGCAGGGCC TGGGTAGCCG GGAAGACCTG TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT 
CTGACCCGCT CCTCATCCTT CTGGGGCGGG TCGAGCATCC AGGTGCAGCA GCGCTCCGGC 
AGCCAGAGCA AAGTCTCCAA GCACATCCGC CTGCCAGCCC CTTGCCCAGG CCTGGAACCC 
AGCTGGGCCA AGGACCCTCA AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCAGCAGT CAGGAAGAAC CCCTGTCCCC ACGGGACTTG 
AAAAAATGCT ACAGTGTAGA GGCCCAGAGC TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC 
GAACAGAGGA GACACTCCAT CGCTGTCAGC TGCCTGGACA GCGGCTCCCA GCCCCGCCTA 
TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG 
AAAAAACTCA GCCCACCCAG TATCTCTATA GACCCCCCGG AGAGCCAGGG CCCTCGGCCC 
CCATGCAGTC CTGGCGTCTG CCTCAGGAGG AGGGCGCCGG CCAGTGACTC GAAGGATCCC 
TCGGCCTCCA GCCCCCTTGA CAGCACGGCT GCCTCACCCT CCCCAAAGAA AGATGCGCTG 
AGTCTCTCTG GTTTGTCTTC TGACCCAACA GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_017314234.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169723.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X30, mRNA.

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CloneID	OMu77715
Clone ID Related Accession (Same CDS sequence)	XM_017314234.2 , XM_017314234.1
Accession Version	XM_017314234.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314234.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGGAGATGC CACCAAGTCT 
GAGTCAGAGC CTGATTTCTT TTCGCCCAGT GTGGATGGTG ATGGGGACAG GAAGAAGCGC 
TTGGCCCTGG TGGCCTTGGG AGAACACTCG GAACTACGAA AGAGCCTTTT GCCACCTCTC 
ATCATCCACA CAGCTGCTAC ACCGATGTCA CTGCCCAAGA GCTCCAGCAC AGGTGTGGGG 
GAAGCACTGG GCTCTGGCTC TCGCCGCACC AGTAGCAGTG GGTCCGCTGA GCCTGGAACT 
GCCCATCATG AGATGAAATC ACCGCCAAGT GCCCGAAGCT CCCCGCACAG TCCCTGGAGC 
GCAGCAAGCA GCTGGACCAG CAGGCGCTCC AGCCGGAACA GCCTGGGCCG GGCCCCCAGC 
CTGAAGCGTA GGAGCCCAAG CGGGGAGCGG AGGTCCCTGC TGTCTGGAGA GGGTCAGGAG 
AGCCAGGATG AGGAGGAGAG TTCAGAAGAG GACCGGGCCA GCCCAGCAGG CAGTGATCAT 
CGCCACAGGG GTTCCTTGGA ACGTGAGGCC AAGAGTTCCT TTGACCTGCC TGACACCCTG 
CAGGTGCCCG GGCTTCATCG AACAGCCAGC GGTCGGAGCT CTGCCTCTGA ACACCAAGAC 
TGTAATGGCA AGTCGGCTTC AGGGCGTTTG GCCCGCACCC TGCGGGCTGA TGACCCCCCA 
CTGGATGGGG ATGATGGCGA TGATGAGGGC AACCTGAGCA AAGGGGAACG CTTACGAGCC 
TGGGTCCGAG CCCGGCTCCC TGCCTGTTGC CGAGAGCGAG ATTCCTGGTC TGCCTATATC 
TTCCCTCCCC AGTCAAGGTT TCGTCTCCTG TGTCACCGGA TCATCACCCA CAAGATGTTT 
GACCATGTGG TCCTCGTCAT CATCTTCCTC AACTGTATCA CCATCGCTAT GGAGCGCCCC 
AAAATTGACC CCCACAGCGC TGAACGCATC TTCCTGACCC TCTCCAACTA CATCTTCACG 
GCAGTCTTCC TGGCTGAAAT GACAGTGAAG GTGGTGGCAC TGGGCTGGTG CTTTGGGGAG 
CAGGCCTACC TGCGCAGCAG CTGGAACGTG CTGGACGGCT TGCTGGTGCT CATCTCTGTC 
ATCGACATCC TGGTGTCCAT GGTCTCTGAC AGCGGCACCA AGATTCTCGG CATGCTGAGG 
GTGCTGCGGC TGCTGCGGAC CCTACGTCCA CTCAGGGTCA TCAGCCGGGC CCAGGGGCTG 
AAGCTGGTGG TAGAGACTCT GATGTCATCC CTCAAACCCA TTGGCAACAT TGTGGTCATC 
TGCTGTGCCT TCTTCATCAT TTTTGGAATT CTTGGGGTGC AGCTCTTCAA AGGGAAGTTC 
TTCGTGTGTC AGGGTGAGGA CACCAGGAAC ATCACTAACA AGTCCGACTG TGCTGAGGCC 
AGTTACCGGT GGGTCCGGCA CAAGTACAAC TTTGACAACC TGGGCCAGGC TCTGATGTCC 
CTGTTTGTGC TGGCCTCCAA GGATGGCTGG GTTGACATCA TGTATGATGG ACTGGATGCT 
GTGGGAGTGG ACCAGCAGCC CATCATGAAC CACAACCCTT GGATGCTGCT CTACTTCATC 
TCCTTCCTCC TCATCGTGGC CTTCTTCGTC CTGAACATGT TTGTGGGCGT GGTGGTGGAG 
AACTTCCATA AGTGCAGGCA GCACCAGGAG GAGGAGGAGG CGCGGCGGCG GGAGGAGAAG 
CGACTAAAGA GGCTGGAGAA AAAGAGAAGG AGTAAGGAGA AGCAGATGGC CGAAGCCCAG 
TGCAAACCCT ACTACTCTGA CTACTCGCGC TTCCGGCTCC TCGTCCACCA CCTGTGTACC 
AGCCACTACC TGGACCTCTT CATCACTGGT GTCATCGGGC TGAATGTGGT CACGATGGCC 
ATGGAACATT ACCAGCAGCC CCAGATCCTG GACGAGGCTC TGAAGATCTG CAACTACATC 
TTTACCGTCA TCTTTGTCTT GGAGTCAGTA TTCAAACTTG TGGCCTTCGG CTTCCGCCGG 
TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC CTGGCTATTG TGCTTCTGTC CATCATGGGC 
ATCACGCTGG AAGAGATTGA GGTCAATGCT TCACTGCCCA TCAACCCCAC CATCATCCGT 
ATCATGAGGG TGCTCCGCAT TGCTCGAGTT CTGAAGCTGT TGAAGATGGC TGTGGGCATG 
CGGGCACTGC TGGACACGGT GATGCAGGCC CTGCCCCAGG TGGGGAACCT GGGACTTCTC 
TTCATGCTAT TATTTTTCAT CTTTGCAGCT CTGGGCGTGG AGCTCTTTGG AGACCTGGAG 
TGTGATGAGA CACACCCTTG TGAGGGCTTG GGCCGGCATG CCACCTTTAG GAACTTTGGT 
ATGGCCTTTC TGACCCTCTT CCGAGTCTCC ACTGGTGACA ACTGGAATGG TATTATGAAG 
GACACCCTCC GGGACTGTGA CCAGGAGTCC ACCTGCTACA ACACCGTCAT CTCACCCATC 
TACTTCGTGT CCTTCGTGCT GACGGCCCAG TTTGTGCTGG TCAACGTGGT CATAGCCGTG 
CTGATGAAGC ACCTGGAAGA GAGCAACAAA GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG 
GAGCTGGAGC TAGAGATGAA GACACTCAGC CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC 
TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT AGCCCTGACA GCCCTAAGCC TGGGGCTCCA 
CACACCACGG CCCACATTGG AGCAGCCTCT TCAGGCTTCT CCCTTGAGCA CCCCACGATG 
GTACCTCACA CTGAGGAGGG GCCAGTCCCC CTAGGACCAG ACCTGCTGAC TGTGAGGAAG 
TCTGGTGTCA GCCGGACACA CTCTCTGCCC AATGACAGCT ACATGTGCCG CAATGGGAGC 
ACTGCCGAGA GATCCCTAGG ACACAGGGGC TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC 
ATCTTGTCTG TTCACTCCCA ACCAGCAGAC ACCAGCTGCA TCCTACAGCT TCCCAAAGAT 
GCACACTATC TGCTCCAGCC TCATGGGGCT CCCACCTGGG GCGCCATCCC TAAACTACCC 
CCACCTGGCC GCTCCCCTCT GGCTCAGAGG CCTCTCAGGC GCCAGGCAGC AATAAGGACT 
GACTCCCTGG ACGTGCAGGG CCTGGGTAGC CGGGAAGACC TGTTGTCAGA GGTGAGTGGG 
CCCTCCTGCC CTCTGACCCG CTCCTCATCC TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG 
CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC AAGCACATCC GCCTGCCAGC CCCTTGCCCA 
GGCCTGGAAC CCAGCTGGGC CAAGGACCCT CAAGAGACCA GAAGCAGCTT AGAGCTGGAC 
ACGGAGCTGA GCTGGATTTC AGGAGACCTC CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC 
CCACGGGACT TGAAAAAATG CTACAGTGTA GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG 
TCCTGGCTAG ACGAACAGAG GAGACACTCC ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC 
CAGCCCCGCC TATGTCCAAG CCCCTCAAGC CTCGGGGGCC AACCTCTTGG GGGCCCTGGG 
AGCCGGCCTA AGAAAAAACT CAGCCCACCC AGTATCTCTA TAGACCCCCC GGAGAGCCAG 
GGCCCTCGGC CCCCATGCAG TCCTGGCGTC TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC 
TCGAAGGATC CCTCGGCCTC CAGCCCCCTT GACAGCACGG CTGCCTCACC CTCCCCAAAG 
AAAGATGCGC TGAGTCTCTC TGGTTTGTCT TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169723.1
CDS	784..7560
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_017314234.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314234.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGGAGATGC CACCAAGTCT 
GAGTCAGAGC CTGATTTCTT TTCGCCCAGT GTGGATGGTG ATGGGGACAG GAAGAAGCGC 
TTGGCCCTGG TGGCCTTGGG AGAACACTCG GAACTACGAA AGAGCCTTTT GCCACCTCTC 
ATCATCCACA CAGCTGCTAC ACCGATGTCA CTGCCCAAGA GCTCCAGCAC AGGTGTGGGG 
GAAGCACTGG GCTCTGGCTC TCGCCGCACC AGTAGCAGTG GGTCCGCTGA GCCTGGAACT 
GCCCATCATG AGATGAAATC ACCGCCAAGT GCCCGAAGCT CCCCGCACAG TCCCTGGAGC 
GCAGCAAGCA GCTGGACCAG CAGGCGCTCC AGCCGGAACA GCCTGGGCCG GGCCCCCAGC 
CTGAAGCGTA GGAGCCCAAG CGGGGAGCGG AGGTCCCTGC TGTCTGGAGA GGGTCAGGAG 
AGCCAGGATG AGGAGGAGAG TTCAGAAGAG GACCGGGCCA GCCCAGCAGG CAGTGATCAT 
CGCCACAGGG GTTCCTTGGA ACGTGAGGCC AAGAGTTCCT TTGACCTGCC TGACACCCTG 
CAGGTGCCCG GGCTTCATCG AACAGCCAGC GGTCGGAGCT CTGCCTCTGA ACACCAAGAC 
TGTAATGGCA AGTCGGCTTC AGGGCGTTTG GCCCGCACCC TGCGGGCTGA TGACCCCCCA 
CTGGATGGGG ATGATGGCGA TGATGAGGGC AACCTGAGCA AAGGGGAACG CTTACGAGCC 
TGGGTCCGAG CCCGGCTCCC TGCCTGTTGC CGAGAGCGAG ATTCCTGGTC TGCCTATATC 
TTCCCTCCCC AGTCAAGGTT TCGTCTCCTG TGTCACCGGA TCATCACCCA CAAGATGTTT 
GACCATGTGG TCCTCGTCAT CATCTTCCTC AACTGTATCA CCATCGCTAT GGAGCGCCCC 
AAAATTGACC CCCACAGCGC TGAACGCATC TTCCTGACCC TCTCCAACTA CATCTTCACG 
GCAGTCTTCC TGGCTGAAAT GACAGTGAAG GTGGTGGCAC TGGGCTGGTG CTTTGGGGAG 
CAGGCCTACC TGCGCAGCAG CTGGAACGTG CTGGACGGCT TGCTGGTGCT CATCTCTGTC 
ATCGACATCC TGGTGTCCAT GGTCTCTGAC AGCGGCACCA AGATTCTCGG CATGCTGAGG 
GTGCTGCGGC TGCTGCGGAC CCTACGTCCA CTCAGGGTCA TCAGCCGGGC CCAGGGGCTG 
AAGCTGGTGG TAGAGACTCT GATGTCATCC CTCAAACCCA TTGGCAACAT TGTGGTCATC 
TGCTGTGCCT TCTTCATCAT TTTTGGAATT CTTGGGGTGC AGCTCTTCAA AGGGAAGTTC 
TTCGTGTGTC AGGGTGAGGA CACCAGGAAC ATCACTAACA AGTCCGACTG TGCTGAGGCC 
AGTTACCGGT GGGTCCGGCA CAAGTACAAC TTTGACAACC TGGGCCAGGC TCTGATGTCC 
CTGTTTGTGC TGGCCTCCAA GGATGGCTGG GTTGACATCA TGTATGATGG ACTGGATGCT 
GTGGGAGTGG ACCAGCAGCC CATCATGAAC CACAACCCTT GGATGCTGCT CTACTTCATC 
TCCTTCCTCC TCATCGTGGC CTTCTTCGTC CTGAACATGT TTGTGGGCGT GGTGGTGGAG 
AACTTCCATA AGTGCAGGCA GCACCAGGAG GAGGAGGAGG CGCGGCGGCG GGAGGAGAAG 
CGACTAAAGA GGCTGGAGAA AAAGAGAAGG AGTAAGGAGA AGCAGATGGC CGAAGCCCAG 
TGCAAACCCT ACTACTCTGA CTACTCGCGC TTCCGGCTCC TCGTCCACCA CCTGTGTACC 
AGCCACTACC TGGACCTCTT CATCACTGGT GTCATCGGGC TGAATGTGGT CACGATGGCC 
ATGGAACATT ACCAGCAGCC CCAGATCCTG GACGAGGCTC TGAAGATCTG CAACTACATC 
TTTACCGTCA TCTTTGTCTT GGAGTCAGTA TTCAAACTTG TGGCCTTCGG CTTCCGCCGG 
TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC CTGGCTATTG TGCTTCTGTC CATCATGGGC 
ATCACGCTGG AAGAGATTGA GGTCAATGCT TCACTGCCCA TCAACCCCAC CATCATCCGT 
ATCATGAGGG TGCTCCGCAT TGCTCGAGTT CTGAAGCTGT TGAAGATGGC TGTGGGCATG 
CGGGCACTGC TGGACACGGT GATGCAGGCC CTGCCCCAGG TGGGGAACCT GGGACTTCTC 
TTCATGCTAT TATTTTTCAT CTTTGCAGCT CTGGGCGTGG AGCTCTTTGG AGACCTGGAG 
TGTGATGAGA CACACCCTTG TGAGGGCTTG GGCCGGCATG CCACCTTTAG GAACTTTGGT 
ATGGCCTTTC TGACCCTCTT CCGAGTCTCC ACTGGTGACA ACTGGAATGG TATTATGAAG 
GACACCCTCC GGGACTGTGA CCAGGAGTCC ACCTGCTACA ACACCGTCAT CTCACCCATC 
TACTTCGTGT CCTTCGTGCT GACGGCCCAG TTTGTGCTGG TCAACGTGGT CATAGCCGTG 
CTGATGAAGC ACCTGGAAGA GAGCAACAAA GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG 
GAGCTGGAGC TAGAGATGAA GACACTCAGC CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC 
TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT AGCCCTGACA GCCCTAAGCC TGGGGCTCCA 
CACACCACGG CCCACATTGG AGCAGCCTCT TCAGGCTTCT CCCTTGAGCA CCCCACGATG 
GTACCTCACA CTGAGGAGGG GCCAGTCCCC CTAGGACCAG ACCTGCTGAC TGTGAGGAAG 
TCTGGTGTCA GCCGGACACA CTCTCTGCCC AATGACAGCT ACATGTGCCG CAATGGGAGC 
ACTGCCGAGA GATCCCTAGG ACACAGGGGC TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC 
ATCTTGTCTG TTCACTCCCA ACCAGCAGAC ACCAGCTGCA TCCTACAGCT TCCCAAAGAT 
GCACACTATC TGCTCCAGCC TCATGGGGCT CCCACCTGGG GCGCCATCCC TAAACTACCC 
CCACCTGGCC GCTCCCCTCT GGCTCAGAGG CCTCTCAGGC GCCAGGCAGC AATAAGGACT 
GACTCCCTGG ACGTGCAGGG CCTGGGTAGC CGGGAAGACC TGTTGTCAGA GGTGAGTGGG 
CCCTCCTGCC CTCTGACCCG CTCCTCATCC TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG 
CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC AAGCACATCC GCCTGCCAGC CCCTTGCCCA 
GGCCTGGAAC CCAGCTGGGC CAAGGACCCT CAAGAGACCA GAAGCAGCTT AGAGCTGGAC 
ACGGAGCTGA GCTGGATTTC AGGAGACCTC CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC 
CCACGGGACT TGAAAAAATG CTACAGTGTA GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG 
TCCTGGCTAG ACGAACAGAG GAGACACTCC ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC 
CAGCCCCGCC TATGTCCAAG CCCCTCAAGC CTCGGGGGCC AACCTCTTGG GGGCCCTGGG 
AGCCGGCCTA AGAAAAAACT CAGCCCACCC AGTATCTCTA TAGACCCCCC GGAGAGCCAG 
GGCCCTCGGC CCCCATGCAG TCCTGGCGTC TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC 
TCGAAGGATC CCTCGGCCTC CAGCCCCCTT GACAGCACGG CTGCCTCACC CTCCCCAAAG 
AAAGATGCGC TGAGTCTCTC TGGTTTGTCT TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_017314234.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169723.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X29, mRNA.

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CloneID	OMu77715
Clone ID Related Accession (Same CDS sequence)	XM_017314234.2 , XM_017314234.1
Accession Version	XM_017314234.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGGAGATGC CACCAAGTCT 
GAGTCAGAGC CTGATTTCTT TTCGCCCAGT GTGGATGGTG ATGGGGACAG GAAGAAGCGC 
TTGGCCCTGG TGGCCTTGGG AGAACACTCG GAACTACGAA AGAGCCTTTT GCCACCTCTC 
ATCATCCACA CAGCTGCTAC ACCGATGTCA CTGCCCAAGA GCTCCAGCAC AGGTGTGGGG 
GAAGCACTGG GCTCTGGCTC TCGCCGCACC AGTAGCAGTG GGTCCGCTGA GCCTGGAACT 
GCCCATCATG AGATGAAATC ACCGCCAAGT GCCCGAAGCT CCCCGCACAG TCCCTGGAGC 
GCAGCAAGCA GCTGGACCAG CAGGCGCTCC AGCCGGAACA GCCTGGGCCG GGCCCCCAGC 
CTGAAGCGTA GGAGCCCAAG CGGGGAGCGG AGGTCCCTGC TGTCTGGAGA GGGTCAGGAG 
AGCCAGGATG AGGAGGAGAG TTCAGAAGAG GACCGGGCCA GCCCAGCAGG CAGTGATCAT 
CGCCACAGGG GTTCCTTGGA ACGTGAGGCC AAGAGTTCCT TTGACCTGCC TGACACCCTG 
CAGGTGCCCG GGCTTCATCG AACAGCCAGC GGTCGGAGCT CTGCCTCTGA ACACCAAGAC 
TGTAATGGCA AGTCGGCTTC AGGGCGTTTG GCCCGCACCC TGCGGGCTGA TGACCCCCCA 
CTGGATGGGG ATGATGGCGA TGATGAGGGC AACCTGAGCA AAGGGGAACG CTTACGAGCC 
TGGGTCCGAG CCCGGCTCCC TGCCTGTTGC CGAGAGCGAG ATTCCTGGTC TGCCTATATC 
TTCCCTCCCC AGTCAAGGTT TCGTCTCCTG TGTCACCGGA TCATCACCCA CAAGATGTTT 
GACCATGTGG TCCTCGTCAT CATCTTCCTC AACTGTATCA CCATCGCTAT GGAGCGCCCC 
AAAATTGACC CCCACAGCGC TGAACGCATC TTCCTGACCC TCTCCAACTA CATCTTCACG 
GCAGTCTTCC TGGCTGAAAT GACAGTGAAG GTGGTGGCAC TGGGCTGGTG CTTTGGGGAG 
CAGGCCTACC TGCGCAGCAG CTGGAACGTG CTGGACGGCT TGCTGGTGCT CATCTCTGTC 
ATCGACATCC TGGTGTCCAT GGTCTCTGAC AGCGGCACCA AGATTCTCGG CATGCTGAGG 
GTGCTGCGGC TGCTGCGGAC CCTACGTCCA CTCAGGGTCA TCAGCCGGGC CCAGGGGCTG 
AAGCTGGTGG TAGAGACTCT GATGTCATCC CTCAAACCCA TTGGCAACAT TGTGGTCATC 
TGCTGTGCCT TCTTCATCAT TTTTGGAATT CTTGGGGTGC AGCTCTTCAA AGGGAAGTTC 
TTCGTGTGTC AGGGTGAGGA CACCAGGAAC ATCACTAACA AGTCCGACTG TGCTGAGGCC 
AGTTACCGGT GGGTCCGGCA CAAGTACAAC TTTGACAACC TGGGCCAGGC TCTGATGTCC 
CTGTTTGTGC TGGCCTCCAA GGATGGCTGG GTTGACATCA TGTATGATGG ACTGGATGCT 
GTGGGAGTGG ACCAGCAGCC CATCATGAAC CACAACCCTT GGATGCTGCT CTACTTCATC 
TCCTTCCTCC TCATCGTGGC CTTCTTCGTC CTGAACATGT TTGTGGGCGT GGTGGTGGAG 
AACTTCCATA AGTGCAGGCA GCACCAGGAG GAGGAGGAGG CGCGGCGGCG GGAGGAGAAG 
CGACTAAAGA GGCTGGAGAA AAAGAGAAGG AGTAAGGAGA AGCAGATGGC CGAAGCCCAG 
TGCAAACCCT ACTACTCTGA CTACTCGCGC TTCCGGCTCC TCGTCCACCA CCTGTGTACC 
AGCCACTACC TGGACCTCTT CATCACTGGT GTCATCGGGC TGAATGTGGT CACGATGGCC 
ATGGAACATT ACCAGCAGCC CCAGATCCTG GACGAGGCTC TGAAGATCTG CAACTACATC 
TTTACCGTCA TCTTTGTCTT GGAGTCAGTA TTCAAACTTG TGGCCTTCGG CTTCCGCCGG 
TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC CTGGCTATTG TGCTTCTGTC CATCATGGGC 
ATCACGCTGG AAGAGATTGA GGTCAATGCT TCACTGCCCA TCAACCCCAC CATCATCCGT 
ATCATGAGGG TGCTCCGCAT TGCTCGAGTT CTGAAGCTGT TGAAGATGGC TGTGGGCATG 
CGGGCACTGC TGGACACGGT GATGCAGGCC CTGCCCCAGG TGGGGAACCT GGGACTTCTC 
TTCATGCTAT TATTTTTCAT CTTTGCAGCT CTGGGCGTGG AGCTCTTTGG AGACCTGGAG 
TGTGATGAGA CACACCCTTG TGAGGGCTTG GGCCGGCATG CCACCTTTAG GAACTTTGGT 
ATGGCCTTTC TGACCCTCTT CCGAGTCTCC ACTGGTGACA ACTGGAATGG TATTATGAAG 
GACACCCTCC GGGACTGTGA CCAGGAGTCC ACCTGCTACA ACACCGTCAT CTCACCCATC 
TACTTCGTGT CCTTCGTGCT GACGGCCCAG TTTGTGCTGG TCAACGTGGT CATAGCCGTG 
CTGATGAAGC ACCTGGAAGA GAGCAACAAA GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG 
GAGCTGGAGC TAGAGATGAA GACACTCAGC CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC 
TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT AGCCCTGACA GCCCTAAGCC TGGGGCTCCA 
CACACCACGG CCCACATTGG AGCAGCCTCT TCAGGCTTCT CCCTTGAGCA CCCCACGATG 
GTACCTCACA CTGAGGAGGG GCCAGTCCCC CTAGGACCAG ACCTGCTGAC TGTGAGGAAG 
TCTGGTGTCA GCCGGACACA CTCTCTGCCC AATGACAGCT ACATGTGCCG CAATGGGAGC 
ACTGCCGAGA GATCCCTAGG ACACAGGGGC TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC 
ATCTTGTCTG TTCACTCCCA ACCAGCAGAC ACCAGCTGCA TCCTACAGCT TCCCAAAGAT 
GCACACTATC TGCTCCAGCC TCATGGGGCT CCCACCTGGG GCGCCATCCC TAAACTACCC 
CCACCTGGCC GCTCCCCTCT GGCTCAGAGG CCTCTCAGGC GCCAGGCAGC AATAAGGACT 
GACTCCCTGG ACGTGCAGGG CCTGGGTAGC CGGGAAGACC TGTTGTCAGA GGTGAGTGGG 
CCCTCCTGCC CTCTGACCCG CTCCTCATCC TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG 
CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC AAGCACATCC GCCTGCCAGC CCCTTGCCCA 
GGCCTGGAAC CCAGCTGGGC CAAGGACCCT CAAGAGACCA GAAGCAGCTT AGAGCTGGAC 
ACGGAGCTGA GCTGGATTTC AGGAGACCTC CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC 
CCACGGGACT TGAAAAAATG CTACAGTGTA GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG 
TCCTGGCTAG ACGAACAGAG GAGACACTCC ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC 
CAGCCCCGCC TATGTCCAAG CCCCTCAAGC CTCGGGGGCC AACCTCTTGG GGGCCCTGGG 
AGCCGGCCTA AGAAAAAACT CAGCCCACCC AGTATCTCTA TAGACCCCCC GGAGAGCCAG 
GGCCCTCGGC CCCCATGCAG TCCTGGCGTC TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC 
TCGAAGGATC CCTCGGCCTC CAGCCCCCTT GACAGCACGG CTGCCTCACC CTCCCCAAAG 
AAAGATGCGC TGAGTCTCTC TGGTTTGTCT TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169723.1
CDS	773..7549
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_017314234.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314234.1

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGGAGATGC CACCAAGTCT 
GAGTCAGAGC CTGATTTCTT TTCGCCCAGT GTGGATGGTG ATGGGGACAG GAAGAAGCGC 
TTGGCCCTGG TGGCCTTGGG AGAACACTCG GAACTACGAA AGAGCCTTTT GCCACCTCTC 
ATCATCCACA CAGCTGCTAC ACCGATGTCA CTGCCCAAGA GCTCCAGCAC AGGTGTGGGG 
GAAGCACTGG GCTCTGGCTC TCGCCGCACC AGTAGCAGTG GGTCCGCTGA GCCTGGAACT 
GCCCATCATG AGATGAAATC ACCGCCAAGT GCCCGAAGCT CCCCGCACAG TCCCTGGAGC 
GCAGCAAGCA GCTGGACCAG CAGGCGCTCC AGCCGGAACA GCCTGGGCCG GGCCCCCAGC 
CTGAAGCGTA GGAGCCCAAG CGGGGAGCGG AGGTCCCTGC TGTCTGGAGA GGGTCAGGAG 
AGCCAGGATG AGGAGGAGAG TTCAGAAGAG GACCGGGCCA GCCCAGCAGG CAGTGATCAT 
CGCCACAGGG GTTCCTTGGA ACGTGAGGCC AAGAGTTCCT TTGACCTGCC TGACACCCTG 
CAGGTGCCCG GGCTTCATCG AACAGCCAGC GGTCGGAGCT CTGCCTCTGA ACACCAAGAC 
TGTAATGGCA AGTCGGCTTC AGGGCGTTTG GCCCGCACCC TGCGGGCTGA TGACCCCCCA 
CTGGATGGGG ATGATGGCGA TGATGAGGGC AACCTGAGCA AAGGGGAACG CTTACGAGCC 
TGGGTCCGAG CCCGGCTCCC TGCCTGTTGC CGAGAGCGAG ATTCCTGGTC TGCCTATATC 
TTCCCTCCCC AGTCAAGGTT TCGTCTCCTG TGTCACCGGA TCATCACCCA CAAGATGTTT 
GACCATGTGG TCCTCGTCAT CATCTTCCTC AACTGTATCA CCATCGCTAT GGAGCGCCCC 
AAAATTGACC CCCACAGCGC TGAACGCATC TTCCTGACCC TCTCCAACTA CATCTTCACG 
GCAGTCTTCC TGGCTGAAAT GACAGTGAAG GTGGTGGCAC TGGGCTGGTG CTTTGGGGAG 
CAGGCCTACC TGCGCAGCAG CTGGAACGTG CTGGACGGCT TGCTGGTGCT CATCTCTGTC 
ATCGACATCC TGGTGTCCAT GGTCTCTGAC AGCGGCACCA AGATTCTCGG CATGCTGAGG 
GTGCTGCGGC TGCTGCGGAC CCTACGTCCA CTCAGGGTCA TCAGCCGGGC CCAGGGGCTG 
AAGCTGGTGG TAGAGACTCT GATGTCATCC CTCAAACCCA TTGGCAACAT TGTGGTCATC 
TGCTGTGCCT TCTTCATCAT TTTTGGAATT CTTGGGGTGC AGCTCTTCAA AGGGAAGTTC 
TTCGTGTGTC AGGGTGAGGA CACCAGGAAC ATCACTAACA AGTCCGACTG TGCTGAGGCC 
AGTTACCGGT GGGTCCGGCA CAAGTACAAC TTTGACAACC TGGGCCAGGC TCTGATGTCC 
CTGTTTGTGC TGGCCTCCAA GGATGGCTGG GTTGACATCA TGTATGATGG ACTGGATGCT 
GTGGGAGTGG ACCAGCAGCC CATCATGAAC CACAACCCTT GGATGCTGCT CTACTTCATC 
TCCTTCCTCC TCATCGTGGC CTTCTTCGTC CTGAACATGT TTGTGGGCGT GGTGGTGGAG 
AACTTCCATA AGTGCAGGCA GCACCAGGAG GAGGAGGAGG CGCGGCGGCG GGAGGAGAAG 
CGACTAAAGA GGCTGGAGAA AAAGAGAAGG AGTAAGGAGA AGCAGATGGC CGAAGCCCAG 
TGCAAACCCT ACTACTCTGA CTACTCGCGC TTCCGGCTCC TCGTCCACCA CCTGTGTACC 
AGCCACTACC TGGACCTCTT CATCACTGGT GTCATCGGGC TGAATGTGGT CACGATGGCC 
ATGGAACATT ACCAGCAGCC CCAGATCCTG GACGAGGCTC TGAAGATCTG CAACTACATC 
TTTACCGTCA TCTTTGTCTT GGAGTCAGTA TTCAAACTTG TGGCCTTCGG CTTCCGCCGG 
TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC CTGGCTATTG TGCTTCTGTC CATCATGGGC 
ATCACGCTGG AAGAGATTGA GGTCAATGCT TCACTGCCCA TCAACCCCAC CATCATCCGT 
ATCATGAGGG TGCTCCGCAT TGCTCGAGTT CTGAAGCTGT TGAAGATGGC TGTGGGCATG 
CGGGCACTGC TGGACACGGT GATGCAGGCC CTGCCCCAGG TGGGGAACCT GGGACTTCTC 
TTCATGCTAT TATTTTTCAT CTTTGCAGCT CTGGGCGTGG AGCTCTTTGG AGACCTGGAG 
TGTGATGAGA CACACCCTTG TGAGGGCTTG GGCCGGCATG CCACCTTTAG GAACTTTGGT 
ATGGCCTTTC TGACCCTCTT CCGAGTCTCC ACTGGTGACA ACTGGAATGG TATTATGAAG 
GACACCCTCC GGGACTGTGA CCAGGAGTCC ACCTGCTACA ACACCGTCAT CTCACCCATC 
TACTTCGTGT CCTTCGTGCT GACGGCCCAG TTTGTGCTGG TCAACGTGGT CATAGCCGTG 
CTGATGAAGC ACCTGGAAGA GAGCAACAAA GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG 
GAGCTGGAGC TAGAGATGAA GACACTCAGC CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC 
TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT AGCCCTGACA GCCCTAAGCC TGGGGCTCCA 
CACACCACGG CCCACATTGG AGCAGCCTCT TCAGGCTTCT CCCTTGAGCA CCCCACGATG 
GTACCTCACA CTGAGGAGGG GCCAGTCCCC CTAGGACCAG ACCTGCTGAC TGTGAGGAAG 
TCTGGTGTCA GCCGGACACA CTCTCTGCCC AATGACAGCT ACATGTGCCG CAATGGGAGC 
ACTGCCGAGA GATCCCTAGG ACACAGGGGC TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC 
ATCTTGTCTG TTCACTCCCA ACCAGCAGAC ACCAGCTGCA TCCTACAGCT TCCCAAAGAT 
GCACACTATC TGCTCCAGCC TCATGGGGCT CCCACCTGGG GCGCCATCCC TAAACTACCC 
CCACCTGGCC GCTCCCCTCT GGCTCAGAGG CCTCTCAGGC GCCAGGCAGC AATAAGGACT 
GACTCCCTGG ACGTGCAGGG CCTGGGTAGC CGGGAAGACC TGTTGTCAGA GGTGAGTGGG 
CCCTCCTGCC CTCTGACCCG CTCCTCATCC TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG 
CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC AAGCACATCC GCCTGCCAGC CCCTTGCCCA 
GGCCTGGAAC CCAGCTGGGC CAAGGACCCT CAAGAGACCA GAAGCAGCTT AGAGCTGGAC 
ACGGAGCTGA GCTGGATTTC AGGAGACCTC CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC 
CCACGGGACT TGAAAAAATG CTACAGTGTA GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG 
TCCTGGCTAG ACGAACAGAG GAGACACTCC ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC 
CAGCCCCGCC TATGTCCAAG CCCCTCAAGC CTCGGGGGCC AACCTCTTGG GGGCCCTGGG 
AGCCGGCCTA AGAAAAAACT CAGCCCACCC AGTATCTCTA TAGACCCCCC GGAGAGCCAG 
GGCCCTCGGC CCCCATGCAG TCCTGGCGTC TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC 
TCGAAGGATC CCTCGGCCTC CAGCCCCCTT GACAGCACGG CTGCCTCACC CTCCCCAAAG 
AAAGATGCGC TGAGTCTCTC TGGTTTGTCT TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_030245496.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101356.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X18, mRNA.

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CloneID	OMu85603
Clone ID Related Accession (Same CDS sequence)	XM_030245496.1
Accession Version	XM_030245496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GAAGCCCAGT GCAAACCCTA CTACTCTGAC TACTCGCGCT TCCGGCTCCT CGTCCACCAC 
CTGTGTACCA GCCACTACCT GGACCTCTTC ATCACTGGTG TCATCGGGCT GAATGTGGTC 
ACGATGGCCA TGGAACATTA CCAGCAGCCC CAGATCCTGG ACGAGGCTCT GAAGATCTGC 
AACTACATCT TTACCGTCAT CTTTGTCTTG GAGTCAGTAT TCAAACTTGT GGCCTTCGGC 
TTCCGCCGGT TCTTCCAGGA CAGGTGGAAC CAGCTGGACC TGGCTATTGT GCTTCTGTCC 
ATCATGGGCA TCACGCTGGA AGAGATTGAG GTCAATGCTT CACTGCCCAT CAACCCCACC 
ATCATCCGTA TCATGAGGGT GCTCCGCATT GCTCGAGTTC TGAAGCTGTT GAAGATGGCT 
GTGGGCATGC GGGCACTGCT GGACACGGTG ATGCAGGCCC TGCCCCAGGT GGGGAACCTG 
GGACTTCTCT TCATGCTATT ATTTTTCATC TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA 
GACCTGGAGT GTGATGAGAC ACACCCTTGT GAGGGCTTGG GCCGGCATGC CACCTTTAGG 
AACTTTGGTA TGGCCTTTCT GACCCTCTTC CGAGTCTCCA CTGGTGACAA CTGGAATGGT 
ATTATGAAGG ACACCCTCCG GGACTGTGAC CAGGAGTCCA CCTGCTACAA CACCGTCATC 
TCACCCATCT ACTTCGTGTC CTTCGTGCTG ACGGCCCAGT TTGTGCTGGT CAACGTGGTC 
ATAGCCGTGC TGATGAAGCA CCTGGAAGAG AGCAACAAAG AGGCCAAGGA GGAGGCGGAG 
TTGGAGGCGG AGCTGGAGCT AGAGATGAAG ACACTCAGCC CGCAGCCCCA CTCCCCGCTG 
GGCAGCCCCT TCCTCTGGCC TGGGGTGGAA GGTGTCAATA GCCCTGACAG CCCTAAGCCT 
GGGGCTCCAC ACACCACGGC CCACATTGGA GCAGCCTCTT CAGGCTTCTC CCTTGAGCAC 
CCCACGATCC CTCTAGCTGA GATGGAGGCT CTGTCTCTGA CGTCAGAGAT TGTGTCTGAA 
CCGTCCTGCT CTCTAGCTCT GACGGATGAC TCTTTGCCTG ATGACACTCA CACACTCTTA 
CTTAGTGCCC TGGAGAGCAA TATGGTACCT CACACTGAGG AGGGGCCAGT CCCCCTAGGA 
CCAGACCTGC TGACTGTGAG GAAGTCTGGT GTCAGCCGGA CACACTCTCT GCCCAATGAC 
AGCTACATGT GCCGCAATGG GAGCACTGCC GAGAGATCCC TAGGACACAG GGGCTGGGGG 
CTCCCCAAAG CCCAGTCAGG CTCCATCTTG TCTGTTCACT CCCAACCAGC AGACACCAGC 
TGCATCCTAC AGCTTCCCAA AGATGCACAC TATCTGCTCC AGCCTCATGG GGCTCCCACC 
TGGGGCGCCA TCCCTAAACT ACCCCCACCT GGCCGCTCCC CTCTGGCTCA GAGGCCTCTC 
AGGCGCCAGG CAGCAATAAG GACTGACTCC CTGGACGTGC AGGGCCTGGG TAGCCGGGAA 
GACCTGTTGT CAGAGGTGAG TGGGCCCTCC TGCCCTCTGA CCCGCTCCTC ATCCTTCTGG 
GGCGGGTCGA GCATCCAGGT GCAGCAGCGC TCCGGCAGCC AGAGCAAAGT CTCCAAGCAC 
ATCCGCCTGC CAGCCCCTTG CCCAGGCCTG GAACCCAGCT GGGCCAAGGA CCCTCAAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
AGCAGTCAGG AAGAACCCCT GTCCCCACGG GACTTGAAAA AATGCTACAG TGTAGAGGCC 
CAGAGCTGCC GGCGCAGGCC TGGGTCCTGG CTAGACGAAC AGAGGAGACA CTCCATCGCT 
GTCAGCTGCC TGGACAGCGG CTCCCAGCCC CGCCTATGTC CAAGCCCCTC AAGCCTCGGG 
GGCCAACCTC TTGGGGGCCC TGGGAGCCGG CCTAAGAAAA AACTCAGCCC ACCCAGTATC 
TCTATAGACC CCCCGGAGAG CCAGGGCCCT CGGCCCCCAT GCAGTCCTGG CGTCTGCCTC 
AGGAGGAGGG CGCCGGCCAG TGACTCGAAG GATCCCTCGG CCTCCAGCCC CCTTGACAGC 
ACGGCTGCCT CACCCTCCCC AAAGAAAGAT GCGCTGAGTC TCTCTGGTTT GTCTTCTGAC 
CCAACAGACC TGGATCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101356.1
CDS	784..7764
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_030245496.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245496.1

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GAAGCCCAGT GCAAACCCTA CTACTCTGAC TACTCGCGCT TCCGGCTCCT CGTCCACCAC 
CTGTGTACCA GCCACTACCT GGACCTCTTC ATCACTGGTG TCATCGGGCT GAATGTGGTC 
ACGATGGCCA TGGAACATTA CCAGCAGCCC CAGATCCTGG ACGAGGCTCT GAAGATCTGC 
AACTACATCT TTACCGTCAT CTTTGTCTTG GAGTCAGTAT TCAAACTTGT GGCCTTCGGC 
TTCCGCCGGT TCTTCCAGGA CAGGTGGAAC CAGCTGGACC TGGCTATTGT GCTTCTGTCC 
ATCATGGGCA TCACGCTGGA AGAGATTGAG GTCAATGCTT CACTGCCCAT CAACCCCACC 
ATCATCCGTA TCATGAGGGT GCTCCGCATT GCTCGAGTTC TGAAGCTGTT GAAGATGGCT 
GTGGGCATGC GGGCACTGCT GGACACGGTG ATGCAGGCCC TGCCCCAGGT GGGGAACCTG 
GGACTTCTCT TCATGCTATT ATTTTTCATC TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA 
GACCTGGAGT GTGATGAGAC ACACCCTTGT GAGGGCTTGG GCCGGCATGC CACCTTTAGG 
AACTTTGGTA TGGCCTTTCT GACCCTCTTC CGAGTCTCCA CTGGTGACAA CTGGAATGGT 
ATTATGAAGG ACACCCTCCG GGACTGTGAC CAGGAGTCCA CCTGCTACAA CACCGTCATC 
TCACCCATCT ACTTCGTGTC CTTCGTGCTG ACGGCCCAGT TTGTGCTGGT CAACGTGGTC 
ATAGCCGTGC TGATGAAGCA CCTGGAAGAG AGCAACAAAG AGGCCAAGGA GGAGGCGGAG 
TTGGAGGCGG AGCTGGAGCT AGAGATGAAG ACACTCAGCC CGCAGCCCCA CTCCCCGCTG 
GGCAGCCCCT TCCTCTGGCC TGGGGTGGAA GGTGTCAATA GCCCTGACAG CCCTAAGCCT 
GGGGCTCCAC ACACCACGGC CCACATTGGA GCAGCCTCTT CAGGCTTCTC CCTTGAGCAC 
CCCACGATCC CTCTAGCTGA GATGGAGGCT CTGTCTCTGA CGTCAGAGAT TGTGTCTGAA 
CCGTCCTGCT CTCTAGCTCT GACGGATGAC TCTTTGCCTG ATGACACTCA CACACTCTTA 
CTTAGTGCCC TGGAGAGCAA TATGGTACCT CACACTGAGG AGGGGCCAGT CCCCCTAGGA 
CCAGACCTGC TGACTGTGAG GAAGTCTGGT GTCAGCCGGA CACACTCTCT GCCCAATGAC 
AGCTACATGT GCCGCAATGG GAGCACTGCC GAGAGATCCC TAGGACACAG GGGCTGGGGG 
CTCCCCAAAG CCCAGTCAGG CTCCATCTTG TCTGTTCACT CCCAACCAGC AGACACCAGC 
TGCATCCTAC AGCTTCCCAA AGATGCACAC TATCTGCTCC AGCCTCATGG GGCTCCCACC 
TGGGGCGCCA TCCCTAAACT ACCCCCACCT GGCCGCTCCC CTCTGGCTCA GAGGCCTCTC 
AGGCGCCAGG CAGCAATAAG GACTGACTCC CTGGACGTGC AGGGCCTGGG TAGCCGGGAA 
GACCTGTTGT CAGAGGTGAG TGGGCCCTCC TGCCCTCTGA CCCGCTCCTC ATCCTTCTGG 
GGCGGGTCGA GCATCCAGGT GCAGCAGCGC TCCGGCAGCC AGAGCAAAGT CTCCAAGCAC 
ATCCGCCTGC CAGCCCCTTG CCCAGGCCTG GAACCCAGCT GGGCCAAGGA CCCTCAAGAG 
ACCAGAAGCA GCTTAGAGCT GGACACGGAG CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC 
AGCAGTCAGG AAGAACCCCT GTCCCCACGG GACTTGAAAA AATGCTACAG TGTAGAGGCC 
CAGAGCTGCC GGCGCAGGCC TGGGTCCTGG CTAGACGAAC AGAGGAGACA CTCCATCGCT 
GTCAGCTGCC TGGACAGCGG CTCCCAGCCC CGCCTATGTC CAAGCCCCTC AAGCCTCGGG 
GGCCAACCTC TTGGGGGCCC TGGGAGCCGG CCTAAGAAAA AACTCAGCCC ACCCAGTATC 
TCTATAGACC CCCCGGAGAG CCAGGGCCCT CGGCCCCCAT GCAGTCCTGG CGTCTGCCTC 
AGGAGGAGGG CGCCGGCCAG TGACTCGAAG GATCCCTCGG CCTCCAGCCC CCTTGACAGC 
ACGGCTGCCT CACCCTCCCC AAAGAAAGAT GCGCTGAGTC TCTCTGGTTT GTCTTCTGAC 
CCAACAGACC TGGATCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_030245497.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101357.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X33, mRNA.

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CloneID	OMu85604
Clone ID Related Accession (Same CDS sequence)	XM_030245497.1
Accession Version	XM_030245497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCATTC TCGTCACATT ACTGCTGGAT ACCTTGCCTA TGCTGGGCAA TGTCCTGCTG 
CTCTGTTTCT TCGTCTTTTT CATCTTTGGC ATCGTTGGTG TTCAGCTGTG GGCAGGGCTA 
CTTCGAAACC GATGCTTCCT CCCTGAGAAT TTCAGCCTCC CGCTGAGTGT GGACTTGGAG 
CCTTACTACC AGACAGAGAA TGAGGACGAG AGCCCCTTCA TCTGCTCTCA GCCTCGGGAG 
AACGGCATGC GATCCTGCAG GAGCGTGCCC ACACTGCGCG GGGAAGGCGG TGGTGGTCCA 
CCCTGCGGTC TGGACTATGA GGCCTATAAC AGTTCCAGCA ATACCACCTG TGTCAACTGG 
AACCAATACT ATACCAACTG CTCTGCAGGC GAGCACAACC CCTTCAAAGG CGCCATCAAC 
TTTGACAACA TTGGCTATGC CTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTCGACATCA TGTACTTTGT GATGGATGCT CATTCCTTCT ACAACTTCAT CTACTTCATT 
CTTCTCATCA TCGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATCGCCACA 
CAGTTCTCTG AGACCAAGCA ACGGGAGAGT CAGCTGATGC GGGAGCAGCG TGTACGATTC 
CTGTCCAATG CTAGCACCCT GGCAAGCTTC TCTGAGCCAG GCAGCTGCTA TGAGGAGCTT 
CTCAAGTACC TGGTGTACAT CCTCCGCAAA GCAGCCCGCA GGCTGGCCCA GGTCTCTAGG 
GCTGTAGGCG TGCGGGCTGG GTTGCTCAGC AGCCCAGTGG CCCGTGGTGG GCAGGAGCCC 
CAGCCCAGTG GCAGCTGCTC TCGTTCACAC CGTCGTCTGT CTGTCCACCA CCTGGTCCAC 
CACCATCACC ACCACCATCA CCACTACCAC CTGGGTAATG GGACGCTCAG AGTTCCCCGG 
GCCAGCCCAG AGATCCAGGA CAGGGATGCC AATGGGTCCC GCTGGCTCAT GCTGCCACCA 
CCCTCTACCC CCACTCCCTC TGGGGGCCCT CCGAGGGGTG CGGAGTCTGT ACACAGCTTC 
TACCATGCTG ACTGCCACTT GGAGCCAGTC CGTTGCCAGG CGCCCCCTCC CAGGTCCCCA 
TCGGAGGCAT CTGGCAGGAC TGTGGGTAGT GGGAAGGTAT ACCCCACTGT GCATACCAGC 
CCTCCACCAG AGATGCTGAA GGATAAGGCA CTAGTGGAGG TGGCCCCCAG CCCTGGGCCC 
CCCACCCTCA CCAGCTTCAA CATCCCACCT GGGCCCTTCA GCTCCATGCA CAAGCTCCTG 
GAGACACAGA GTACGGGTGA GAGCGTGGGA GCCTGCCATA GCTCCTGCAA AATCTCCAGC 
CCTTGCTCCA AGGCAGACAG TGGAGCCTGT GGGCCGGACA GTTGTCCCTA CTGTGCCCGG 
ACAGGAGCAG GGGAGCCAGA GTCCGCTGAC CATGAAATGC CTGACTCAGA CAGTGAGGCT 
GTGTATGAGT TCACACAGGA CGCTCAGCAC AGCGACCTCC GGGATCCCCA CAGACGGCGA 
CGGCCGAGCC TGGGCCCAGA TGCAGAGCCT AGTTCTGTGC TGGCCTTCTG GAGGCTGATC 
TGCGACACAT TCCGGAAGAT CGTAGATAGC AAATACTTTG GCCGGGGAAT CATGATCGCT 
ATCCTGGTCA ATACCCTCAG CATGGGCATC GAGTACCACG AGCAGCCCGA GGAGCTCACC 
AACGCCCTGG AAATCAGCAA CATCGTCTTC ACCAGCCTCT TCGCCTTGGA GATGCTGCTG 
AAGCTGCTCG TCTACGGTCC CTTCGGCTAC ATTAAGAATC CCTACAACAT CTTTGATGGC 
GTCATTGTGG TCATCAGCGT GTGGGAGATT GTGGGCCAGC AGGGAGGTGG CCTGTCGGTG 
CTGCGGACCT TCCGCCTGAT GCGGGTGCTG AAGCTAGTTC GCTTCCTGCC GGCACTGCAG 
CGGCAGCTCG TGGTGCTCAT GAAGACCATG GACAACGTGG CCACCTTCTG CATGCTGCTC 
ATGCTCTTCA TCTTCATCTT CAGCATCCTG GGCATGCACC TTTTTGGTTG CAAGTTCGCA 
TCTGAACGGG ATGGGGACAC GTTGCCAGAC CGGAAGAATT TTGACTCCCT GCTCTGGGCC 
ATTGTCACTG TCTTTCAGAT TCTGACTCAG GAAGACTGGA ATAAAGTCCT TTACAACGGC 
ATGGCCTCCA CGTCATCTTG GGCTGCTCTT TACTTCATCG CCCTCATGAC TTTTGGCAAC 
TACGTGCTCT TTAACCTGCT TGTCGCCATT CTCGTGGAGG GTTTCCAGGC AGAGGAAATC 
GGCAAACGGG AAGATACGAG TGGACAGTTA AGCTGTATTC AGCTGCCTGT CAACTCCCAG 
GGGGGAGATG CCACCAAGTC TGAGTCAGAG CCTGATTTCT TTTCGCCCAG TGTGGATGGT 
GATGGGGACA GGAAGAAGCG CTTGGCCCTG GTGGCCTTGG GAGAACACTC GGAACTACGA 
AAGAGCCTTT TGCCACCTCT CATCATCCAC ACAGCTGCTA CACCGATGTC ACTGCCCAAG 
AGCTCCAGCA CAGGTGTGGG GGAAGCACTG GGCTCTGGCT CTCGCCGCAC CAGTAGCAGT 
GGGTCCGCTG AGCCTGGAAC TGCCCATCAT GAGATGAAAT CACCGCCAAG TGCCCGAAGC 
TCCCCGCACA GTCCCTGGAG CGCAGCAAGC AGCTGGACCA GCAGGCGCTC CAGCCGGAAC 
AGCCTGGGCC GGGCCCCCAG CCTGAAGCGT AGGAGCCCAA GCGGGGAGCG GAGGTCCCTG 
CTGTCTGGAG AGGGTCAGGA GAGCCAGGAT GAGGAGGAGA GTTCAGAAGA GGACCGGGCC 
AGCCCAGCAG GCAGTGATCA TCGCCACAGG GGTTCCTTGG AACGTGAGGC CAAGAGTTCC 
TTTGACCTGC CTGACACCCT GCAGGTGCCC GGGCTTCATC GAACAGCCAG CGGTCGGAGC 
TCTGCCTCTG AACACCAAGA CTGTAATGGC AAGTCGGCTT CAGGGCGTTT GGCCCGCACC 
CTGCGGGCTG ATGACCCCCC ACTGGATGGG GATGATGGCG ATGATGAGGG CAACCTGAGC 
AAAGGGGAAC GCTTACGAGC CTGGGTCCGA GCCCGGCTCC CTGCCTGTTG CCGAGAGCGA 
GATTCCTGGT CTGCCTATAT CTTCCCTCCC CAGTCAAGGT TTCGTCTCCT GTGTCACCGG 
ATCATCACCC ACAAGATGTT TGACCATGTG GTCCTCGTCA TCATCTTCCT CAACTGTATC 
ACCATCGCTA TGGAGCGCCC CAAAATTGAC CCCCACAGCG CTGAACGCAT CTTCCTGACC 
CTCTCCAACT ACATCTTCAC GGCAGTCTTC CTGGCTGAAA TGACAGTGAA GGTGGTGGCA 
CTGGGCTGGT GCTTTGGGGA GCAGGCCTAC CTGCGCAGCA GCTGGAACGT GCTGGACGGC 
TTGCTGGTGC TCATCTCTGT CATCGACATC CTGGTGTCCA TGGTCTCTGA CAGCGGCACC 
AAGATTCTCG GCATGCTGAG GGTGCTGCGG CTGCTGCGGA CCCTACGTCC ACTCAGGGTC 
ATCAGCCGGG CCCAGGGGCT GAAGCTGGTG GTAGAGACTC TGATGTCATC CCTCAAACCC 
ATTGGCAACA TTGTGGTCAT CTGCTGTGCC TTCTTCATCA TTTTTGGAAT TCTTGGGGTG 
CAGCTCTTCA AAGGGAAGTT CTTCGTGTGT CAGGGTGAGG ACACCAGGAA CATCACTAAC 
AAGTCCGACT GTGCTGAGGC CAGTTACCGG TGGGTCCGGC ACAAGTACAA CTTTGACAAC 
CTGGGCCAGG CTCTGATGTC CCTGTTTGTG CTGGCCTCCA AGGATGGCTG GGTTGACATC 
ATGTATGATG GACTGGATGC TGTGGGAGTG GACCAGCAGC CCATCATGAA CCACAACCCT 
TGGATGCTGC TCTACTTCAT CTCCTTCCTC CTCATCGTGG CCTTCTTCGT CCTGAACATG 
TTTGTGGGCG TGGTGGTGGA GAACTTCCAT AAGTGCAGGC AGCACCAGGA GGAGGAGGAG 
GCGCGGCGGC GGGAGGAGAA GCGACTAAAG AGGCTGGAGA AAAAGAGAAG GAGTAAGGAG 
AAGCAGATGG CCGATCTAAT GTTGGACGAT GTAATTGCTT CCGGCAGCTC AGCCAGCGCT 
GCGTCAGAAG CCCAGTGCAA ACCCTACTAC TCTGACTACT CGCGCTTCCG GCTCCTCGTC 
CACCACCTGT GTACCAGCCA CTACCTGGAC CTCTTCATCA CTGGTGTCAT CGGGCTGAAT 
GTGGTCACGA TGGCCATGGA ACATTACCAG CAGCCCCAGA TCCTGGACGA GGCTCTGAAG 
ATCTGCAACT ACATCTTTAC CGTCATCTTT GTCTTGGAGT CAGTATTCAA ACTTGTGGCC 
TTCGGCTTCC GCCGGTTCTT CCAGGACAGG TGGAACCAGC TGGACCTGGC TATTGTGCTT 
CTGTCCATCA TGGGCATCAC GCTGGAAGAG ATTGAGGTCA ATGCTTCACT GCCCATCAAC 
CCCACCATCA TCCGTATCAT GAGGGTGCTC CGCATTGCTC GAGTTCTGAA GCTGTTGAAG 
ATGGCTGTGG GCATGCGGGC ACTGCTGGAC ACGGTGATGC AGGCCCTGCC CCAGGTGGGG 
AACCTGGGAC TTCTCTTCAT GCTATTATTT TTCATCTTTG CAGCTCTGGG CGTGGAGCTC 
TTTGGAGACC TGGAGTGTGA TGAGACACAC CCTTGTGAGG GCTTGGGCCG GCATGCCACC 
TTTAGGAACT TTGGTATGGC CTTTCTGACC CTCTTCCGAG TCTCCACTGG TGACAACTGG 
AATGGTATTA TGAAGGACAC CCTCCGGGAC TGTGACCAGG AGTCCACCTG CTACAACACC 
GTCATCTCAC CCATCTACTT CGTGTCCTTC GTGCTGACGG CCCAGTTTGT GCTGGTCAAC 
GTGGTCATAG CCGTGCTGAT GAAGCACCTG GAAGAGAGCA ACAAAGAGGC CAAGGAGGAG 
GCGGAGTTGG AGGCGGAGCT GGAGCTAGAG ATGAAGACAC TCAGCCCGCA GCCCCACTCC 
CCGCTGGGCA GCCCCTTCCT CTGGCCTGGG GTGGAAGGTG TCAATAGCCC TGACAGCCCT 
AAGCCTGGGG CTCCACACAC CACGGCCCAC ATTGGAGCAG CCTCTTCAGG CTTCTCCCTT 
GAGCACCCCA CGGACAGACA GCTGTTTGAC ACCATCTCCC TGCTGATCCA GGGCTCCCTG 
GAGGGGGAGC TGAAGCTGAT GGACGAGCTG GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC 
CCTTCCGCCC CCAGCCCGGG CGACTCCGAC CCACAGATCC CTCTAGCTGA GATGGAGGCT 
CTGTCTCTGA CGTCAGAGAT TGTGTCTGAA CCGTCCTGCT CTCTAGCTCT GACGGATGAC 
TCTTTGCCTG ATGACACTCA CACACTCTTA CTTAGTGCCC TGGAGAGCAA TATGGTACCT 
CACACTGAGG AGGGGCCAGT CCCCCTAGGA CCAGACCTGC TGACTGTGAG GAAGTCTGGT 
GTCAGCCGGA CACACTCTCT GCCCAATGAC AGCTACATGT GCCGCAATGG GAGCACTGCC 
GAGAGATCCC TAGGACACAG GGGCTGGGGG CTCCCCAAAG CCCAGTCAGG CTCCATCTTG 
TCTGTTCACT CCCAACCAGC AGACACCAGC TGCATCCTAC AGCTTCCCAA AGATGCACAC 
TATCTGCTCC AGCCTCATGG GGCTCCCACC TGGGGCGCCA TCCCTAAACT ACCCCCACCT 
GGCCGCTCCC CTCTGGCTCA GAGGCCTCTC AGGCGCCAGG CAGCAATAAG GACTGACTCC 
CTGGACGTGC AGGGCCTGGG TAGCCGGGAA GACCTGTTGT CAGAGGTGAG TGGGCCCTCC 
TGCCCTCTGA CCCGCTCCTC ATCCTTCTGG GGCGGGTCGA GCATCCAGGT GCAGCAGCGC 
TCCGGCAGCC AGAGCAAAGT CTCCAAGCAC ATCCGCCTGC CAGCCCCTTG CCCAGGCCTG 
GAACCCAGCT GGGCCAAGGA CCCTCAAGAG ACCAGAAGCA GCTTAGAGCT GGACACGGAG 
CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC AGCAGTCAGG AAGAACCCCT GTCCCCACGG 
GACTTGAAAA AATGCTACAG TGTAGAGGCC CAGAGCTGCC GGCGCAGGCC TGGGTCCTGG 
CTAGACGAAC AGAGGAGACA CTCCATCGCT GTCAGCTGCC TGGACAGCGG CTCCCAGCCC 
CGCCTATGTC CAAGCCCCTC AAGCCTCGGG GGCCAACCTC TTGGGGGCCC TGGGAGCCGG 
CCTAAGAAAA AACTCAGCCC ACCCAGTATC TCTATAGACC CCCCGGAGAG CCAGGGCCCT 
CGGCCCCCAT GCAGTCCTGG CGTCTGCCTC AGGAGGAGGG CGCCGGCCAG TGACTCGAAG 
GATCCCTCGG CCTCCAGCCC CCTTGACAGC ACGGCTGCCT CACCCTCCCC AAAGAAAGAT 
GCGCTGAGTC TCTCTGGTTT GTCTTCTGAC CCAACAGACC TGGATCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101357.1
CDS	1263..7853
Translation	MRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_030245497.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245497.1

ORF Insert Sequence:

ATGCGCATTC TCGTCACATT ACTGCTGGAT ACCTTGCCTA TGCTGGGCAA TGTCCTGCTG 
CTCTGTTTCT TCGTCTTTTT CATCTTTGGC ATCGTTGGTG TTCAGCTGTG GGCAGGGCTA 
CTTCGAAACC GATGCTTCCT CCCTGAGAAT TTCAGCCTCC CGCTGAGTGT GGACTTGGAG 
CCTTACTACC AGACAGAGAA TGAGGACGAG AGCCCCTTCA TCTGCTCTCA GCCTCGGGAG 
AACGGCATGC GATCCTGCAG GAGCGTGCCC ACACTGCGCG GGGAAGGCGG TGGTGGTCCA 
CCCTGCGGTC TGGACTATGA GGCCTATAAC AGTTCCAGCA ATACCACCTG TGTCAACTGG 
AACCAATACT ATACCAACTG CTCTGCAGGC GAGCACAACC CCTTCAAAGG CGCCATCAAC 
TTTGACAACA TTGGCTATGC CTGGATCGCC ATCTTCCAGG TCATCACACT GGAGGGCTGG 
GTCGACATCA TGTACTTTGT GATGGATGCT CATTCCTTCT ACAACTTCAT CTACTTCATT 
CTTCTCATCA TCGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT GATCGCCACA 
CAGTTCTCTG AGACCAAGCA ACGGGAGAGT CAGCTGATGC GGGAGCAGCG TGTACGATTC 
CTGTCCAATG CTAGCACCCT GGCAAGCTTC TCTGAGCCAG GCAGCTGCTA TGAGGAGCTT 
CTCAAGTACC TGGTGTACAT CCTCCGCAAA GCAGCCCGCA GGCTGGCCCA GGTCTCTAGG 
GCTGTAGGCG TGCGGGCTGG GTTGCTCAGC AGCCCAGTGG CCCGTGGTGG GCAGGAGCCC 
CAGCCCAGTG GCAGCTGCTC TCGTTCACAC CGTCGTCTGT CTGTCCACCA CCTGGTCCAC 
CACCATCACC ACCACCATCA CCACTACCAC CTGGGTAATG GGACGCTCAG AGTTCCCCGG 
GCCAGCCCAG AGATCCAGGA CAGGGATGCC AATGGGTCCC GCTGGCTCAT GCTGCCACCA 
CCCTCTACCC CCACTCCCTC TGGGGGCCCT CCGAGGGGTG CGGAGTCTGT ACACAGCTTC 
TACCATGCTG ACTGCCACTT GGAGCCAGTC CGTTGCCAGG CGCCCCCTCC CAGGTCCCCA 
TCGGAGGCAT CTGGCAGGAC TGTGGGTAGT GGGAAGGTAT ACCCCACTGT GCATACCAGC 
CCTCCACCAG AGATGCTGAA GGATAAGGCA CTAGTGGAGG TGGCCCCCAG CCCTGGGCCC 
CCCACCCTCA CCAGCTTCAA CATCCCACCT GGGCCCTTCA GCTCCATGCA CAAGCTCCTG 
GAGACACAGA GTACGGGTGA GAGCGTGGGA GCCTGCCATA GCTCCTGCAA AATCTCCAGC 
CCTTGCTCCA AGGCAGACAG TGGAGCCTGT GGGCCGGACA GTTGTCCCTA CTGTGCCCGG 
ACAGGAGCAG GGGAGCCAGA GTCCGCTGAC CATGAAATGC CTGACTCAGA CAGTGAGGCT 
GTGTATGAGT TCACACAGGA CGCTCAGCAC AGCGACCTCC GGGATCCCCA CAGACGGCGA 
CGGCCGAGCC TGGGCCCAGA TGCAGAGCCT AGTTCTGTGC TGGCCTTCTG GAGGCTGATC 
TGCGACACAT TCCGGAAGAT CGTAGATAGC AAATACTTTG GCCGGGGAAT CATGATCGCT 
ATCCTGGTCA ATACCCTCAG CATGGGCATC GAGTACCACG AGCAGCCCGA GGAGCTCACC 
AACGCCCTGG AAATCAGCAA CATCGTCTTC ACCAGCCTCT TCGCCTTGGA GATGCTGCTG 
AAGCTGCTCG TCTACGGTCC CTTCGGCTAC ATTAAGAATC CCTACAACAT CTTTGATGGC 
GTCATTGTGG TCATCAGCGT GTGGGAGATT GTGGGCCAGC AGGGAGGTGG CCTGTCGGTG 
CTGCGGACCT TCCGCCTGAT GCGGGTGCTG AAGCTAGTTC GCTTCCTGCC GGCACTGCAG 
CGGCAGCTCG TGGTGCTCAT GAAGACCATG GACAACGTGG CCACCTTCTG CATGCTGCTC 
ATGCTCTTCA TCTTCATCTT CAGCATCCTG GGCATGCACC TTTTTGGTTG CAAGTTCGCA 
TCTGAACGGG ATGGGGACAC GTTGCCAGAC CGGAAGAATT TTGACTCCCT GCTCTGGGCC 
ATTGTCACTG TCTTTCAGAT TCTGACTCAG GAAGACTGGA ATAAAGTCCT TTACAACGGC 
ATGGCCTCCA CGTCATCTTG GGCTGCTCTT TACTTCATCG CCCTCATGAC TTTTGGCAAC 
TACGTGCTCT TTAACCTGCT TGTCGCCATT CTCGTGGAGG GTTTCCAGGC AGAGGAAATC 
GGCAAACGGG AAGATACGAG TGGACAGTTA AGCTGTATTC AGCTGCCTGT CAACTCCCAG 
GGGGGAGATG CCACCAAGTC TGAGTCAGAG CCTGATTTCT TTTCGCCCAG TGTGGATGGT 
GATGGGGACA GGAAGAAGCG CTTGGCCCTG GTGGCCTTGG GAGAACACTC GGAACTACGA 
AAGAGCCTTT TGCCACCTCT CATCATCCAC ACAGCTGCTA CACCGATGTC ACTGCCCAAG 
AGCTCCAGCA CAGGTGTGGG GGAAGCACTG GGCTCTGGCT CTCGCCGCAC CAGTAGCAGT 
GGGTCCGCTG AGCCTGGAAC TGCCCATCAT GAGATGAAAT CACCGCCAAG TGCCCGAAGC 
TCCCCGCACA GTCCCTGGAG CGCAGCAAGC AGCTGGACCA GCAGGCGCTC CAGCCGGAAC 
AGCCTGGGCC GGGCCCCCAG CCTGAAGCGT AGGAGCCCAA GCGGGGAGCG GAGGTCCCTG 
CTGTCTGGAG AGGGTCAGGA GAGCCAGGAT GAGGAGGAGA GTTCAGAAGA GGACCGGGCC 
AGCCCAGCAG GCAGTGATCA TCGCCACAGG GGTTCCTTGG AACGTGAGGC CAAGAGTTCC 
TTTGACCTGC CTGACACCCT GCAGGTGCCC GGGCTTCATC GAACAGCCAG CGGTCGGAGC 
TCTGCCTCTG AACACCAAGA CTGTAATGGC AAGTCGGCTT CAGGGCGTTT GGCCCGCACC 
CTGCGGGCTG ATGACCCCCC ACTGGATGGG GATGATGGCG ATGATGAGGG CAACCTGAGC 
AAAGGGGAAC GCTTACGAGC CTGGGTCCGA GCCCGGCTCC CTGCCTGTTG CCGAGAGCGA 
GATTCCTGGT CTGCCTATAT CTTCCCTCCC CAGTCAAGGT TTCGTCTCCT GTGTCACCGG 
ATCATCACCC ACAAGATGTT TGACCATGTG GTCCTCGTCA TCATCTTCCT CAACTGTATC 
ACCATCGCTA TGGAGCGCCC CAAAATTGAC CCCCACAGCG CTGAACGCAT CTTCCTGACC 
CTCTCCAACT ACATCTTCAC GGCAGTCTTC CTGGCTGAAA TGACAGTGAA GGTGGTGGCA 
CTGGGCTGGT GCTTTGGGGA GCAGGCCTAC CTGCGCAGCA GCTGGAACGT GCTGGACGGC 
TTGCTGGTGC TCATCTCTGT CATCGACATC CTGGTGTCCA TGGTCTCTGA CAGCGGCACC 
AAGATTCTCG GCATGCTGAG GGTGCTGCGG CTGCTGCGGA CCCTACGTCC ACTCAGGGTC 
ATCAGCCGGG CCCAGGGGCT GAAGCTGGTG GTAGAGACTC TGATGTCATC CCTCAAACCC 
ATTGGCAACA TTGTGGTCAT CTGCTGTGCC TTCTTCATCA TTTTTGGAAT TCTTGGGGTG 
CAGCTCTTCA AAGGGAAGTT CTTCGTGTGT CAGGGTGAGG ACACCAGGAA CATCACTAAC 
AAGTCCGACT GTGCTGAGGC CAGTTACCGG TGGGTCCGGC ACAAGTACAA CTTTGACAAC 
CTGGGCCAGG CTCTGATGTC CCTGTTTGTG CTGGCCTCCA AGGATGGCTG GGTTGACATC 
ATGTATGATG GACTGGATGC TGTGGGAGTG GACCAGCAGC CCATCATGAA CCACAACCCT 
TGGATGCTGC TCTACTTCAT CTCCTTCCTC CTCATCGTGG CCTTCTTCGT CCTGAACATG 
TTTGTGGGCG TGGTGGTGGA GAACTTCCAT AAGTGCAGGC AGCACCAGGA GGAGGAGGAG 
GCGCGGCGGC GGGAGGAGAA GCGACTAAAG AGGCTGGAGA AAAAGAGAAG GAGTAAGGAG 
AAGCAGATGG CCGATCTAAT GTTGGACGAT GTAATTGCTT CCGGCAGCTC AGCCAGCGCT 
GCGTCAGAAG CCCAGTGCAA ACCCTACTAC TCTGACTACT CGCGCTTCCG GCTCCTCGTC 
CACCACCTGT GTACCAGCCA CTACCTGGAC CTCTTCATCA CTGGTGTCAT CGGGCTGAAT 
GTGGTCACGA TGGCCATGGA ACATTACCAG CAGCCCCAGA TCCTGGACGA GGCTCTGAAG 
ATCTGCAACT ACATCTTTAC CGTCATCTTT GTCTTGGAGT CAGTATTCAA ACTTGTGGCC 
TTCGGCTTCC GCCGGTTCTT CCAGGACAGG TGGAACCAGC TGGACCTGGC TATTGTGCTT 
CTGTCCATCA TGGGCATCAC GCTGGAAGAG ATTGAGGTCA ATGCTTCACT GCCCATCAAC 
CCCACCATCA TCCGTATCAT GAGGGTGCTC CGCATTGCTC GAGTTCTGAA GCTGTTGAAG 
ATGGCTGTGG GCATGCGGGC ACTGCTGGAC ACGGTGATGC AGGCCCTGCC CCAGGTGGGG 
AACCTGGGAC TTCTCTTCAT GCTATTATTT TTCATCTTTG CAGCTCTGGG CGTGGAGCTC 
TTTGGAGACC TGGAGTGTGA TGAGACACAC CCTTGTGAGG GCTTGGGCCG GCATGCCACC 
TTTAGGAACT TTGGTATGGC CTTTCTGACC CTCTTCCGAG TCTCCACTGG TGACAACTGG 
AATGGTATTA TGAAGGACAC CCTCCGGGAC TGTGACCAGG AGTCCACCTG CTACAACACC 
GTCATCTCAC CCATCTACTT CGTGTCCTTC GTGCTGACGG CCCAGTTTGT GCTGGTCAAC 
GTGGTCATAG CCGTGCTGAT GAAGCACCTG GAAGAGAGCA ACAAAGAGGC CAAGGAGGAG 
GCGGAGTTGG AGGCGGAGCT GGAGCTAGAG ATGAAGACAC TCAGCCCGCA GCCCCACTCC 
CCGCTGGGCA GCCCCTTCCT CTGGCCTGGG GTGGAAGGTG TCAATAGCCC TGACAGCCCT 
AAGCCTGGGG CTCCACACAC CACGGCCCAC ATTGGAGCAG CCTCTTCAGG CTTCTCCCTT 
GAGCACCCCA CGGACAGACA GCTGTTTGAC ACCATCTCCC TGCTGATCCA GGGCTCCCTG 
GAGGGGGAGC TGAAGCTGAT GGACGAGCTG GCAGGCCCAG GGGGCCAGCC CTCTGCCTTC 
CCTTCCGCCC CCAGCCCGGG CGACTCCGAC CCACAGATCC CTCTAGCTGA GATGGAGGCT 
CTGTCTCTGA CGTCAGAGAT TGTGTCTGAA CCGTCCTGCT CTCTAGCTCT GACGGATGAC 
TCTTTGCCTG ATGACACTCA CACACTCTTA CTTAGTGCCC TGGAGAGCAA TATGGTACCT 
CACACTGAGG AGGGGCCAGT CCCCCTAGGA CCAGACCTGC TGACTGTGAG GAAGTCTGGT 
GTCAGCCGGA CACACTCTCT GCCCAATGAC AGCTACATGT GCCGCAATGG GAGCACTGCC 
GAGAGATCCC TAGGACACAG GGGCTGGGGG CTCCCCAAAG CCCAGTCAGG CTCCATCTTG 
TCTGTTCACT CCCAACCAGC AGACACCAGC TGCATCCTAC AGCTTCCCAA AGATGCACAC 
TATCTGCTCC AGCCTCATGG GGCTCCCACC TGGGGCGCCA TCCCTAAACT ACCCCCACCT 
GGCCGCTCCC CTCTGGCTCA GAGGCCTCTC AGGCGCCAGG CAGCAATAAG GACTGACTCC 
CTGGACGTGC AGGGCCTGGG TAGCCGGGAA GACCTGTTGT CAGAGGTGAG TGGGCCCTCC 
TGCCCTCTGA CCCGCTCCTC ATCCTTCTGG GGCGGGTCGA GCATCCAGGT GCAGCAGCGC 
TCCGGCAGCC AGAGCAAAGT CTCCAAGCAC ATCCGCCTGC CAGCCCCTTG CCCAGGCCTG 
GAACCCAGCT GGGCCAAGGA CCCTCAAGAG ACCAGAAGCA GCTTAGAGCT GGACACGGAG 
CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC AGCAGTCAGG AAGAACCCCT GTCCCCACGG 
GACTTGAAAA AATGCTACAG TGTAGAGGCC CAGAGCTGCC GGCGCAGGCC TGGGTCCTGG 
CTAGACGAAC AGAGGAGACA CTCCATCGCT GTCAGCTGCC TGGACAGCGG CTCCCAGCCC 
CGCCTATGTC CAAGCCCCTC AAGCCTCGGG GGCCAACCTC TTGGGGGCCC TGGGAGCCGG 
CCTAAGAAAA AACTCAGCCC ACCCAGTATC TCTATAGACC CCCCGGAGAG CCAGGGCCCT 
CGGCCCCCAT GCAGTCCTGG CGTCTGCCTC AGGAGGAGGG CGCCGGCCAG TGACTCGAAG 
GATCCCTCGG CCTCCAGCCC CCTTGACAGC ACGGCTGCCT CACCCTCCCC AAAGAAAGAT 
GCGCTGAGTC TCTCTGGTTT GTCTTCTGAC CCAACAGACC TGGATCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( NM_001177890.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171361.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant 4, mRNA.

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CloneID	OMu16041
Clone ID Related Accession (Same CDS sequence)	NM_001177890.1
Accession Version	NM_001177890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF536661.1, BC057399.1, CN667053.1, AK129294.1, BU708898.1, AJ012569.1 and AW457316.1. Transcript Variant: This variant (4) lacks an alternate, in-frame coding segment and uses two different splice sites in the coding region, compared to variant 1. The resulting protein (isoform d) is shorter when it is compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057399.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
TTCTCTGAGA CCAAGCAACG GGAGAGTCAG CTGATGCGGG AGCAGCGTGT ACGATTCCTG 
TCCAATGCTA GCACCCTGGC AAGCTTCTCT GAGCCAGGCA GCTGCTATGA GGAGCTTCTC 
AAGTACCTGG TGTACATCCT CCGCAAAGCA GCCCGCAGGC TGGCCCAGGT CTCTAGGGCT 
GTAGGCGTGC GGGCTGGGTT GCTCAGCAGC CCAGTGGCCC GTGGTGGGCA GGAGCCCCAG 
CCCAGTGGCA GCTGCTCTCG TTCACACCGT CGTCTGTCTG TCCACCACCT GGTCCACCAC 
CATCACCACC ACCATCACCA CTACCACCTG GGTAATGGGA CGCTCAGAGT TCCCCGGGCC 
AGCCCAGAGA TCCAGGACAG GGATGCCAAT GGGTCCCGCT GGCTCATGCT GCCACCACCC 
TCTACCCCCA CTCCCTCTGG GGGCCCTCCG AGGGGTGCGG AGTCTGTACA CAGCTTCTAC 
CATGCTGACT GCCACTTGGA GCCAGTCCGT TGCCAGGCGC CCCCTCCCAG GTCCCCATCG 
GAGGCATCTG GCAGGACTGT GGGTAGTGGG AAGGTATACC CCACTGTGCA TACCAGCCCT 
CCACCAGAGA TGCTGAAGGA TAAGGCACTA GTGGAGGTGG CCCCCAGCCC TGGGCCCCCC 
ACCCTCACCA GCTTCAACAT CCCACCTGGG CCCTTCAGCT CCATGCACAA GCTCCTGGAG 
ACACAGAGTA CGGGAGCCTG CCATAGCTCC TGCAAAATCT CCAGCCCTTG CTCCAAGGCA 
GACAGTGGAG CCTGTGGGCC GGACAGTTGT CCCTACTGTG CCCGGACAGG AGCAGGGGAG 
CCAGAGTCCG CTGACCATGA AATGCCTGAC TCAGACAGTG AGGCTGTGTA TGAGTTCACA 
CAGGACGCTC AGCACAGCGA CCTCCGGGAT CCCCACAGAC GGCGACGGCC GAGCCTGGGC 
CCAGATGCAG AGCCTAGTTC TGTGCTGGCC TTCTGGAGGC TGATCTGCGA CACATTCCGG 
AAGATCGTAG ATAGCAAATA CTTTGGCCGG GGAATCATGA TCGCTATCCT GGTCAATACC 
CTCAGCATGG GCATCGAGTA CCACGAGCAG CCCGAGGAGC TCACCAACGC CCTGGAAATC 
AGCAACATCG TCTTCACCAG CCTCTTCGCC TTGGAGATGC TGCTGAAGCT GCTCGTCTAC 
GGTCCCTTCG GCTACATTAA GAATCCCTAC AACATCTTTG ATGGCGTCAT TGTGGTCATC 
AGCGTGTGGG AGATTGTGGG CCAGCAGGGA GGTGGCCTGT CGGTGCTGCG GACCTTCCGC 
CTGATGCGGG TGCTGAAGCT AGTTCGCTTC CTGCCGGCAC TGCAGCGGCA GCTCGTGGTG 
CTCATGAAGA CCATGGACAA CGTGGCCACC TTCTGCATGC TGCTCATGCT CTTCATCTTC 
ATCTTCAGCA TCCTGGGCAT GCACCTTTTT GGTTGCAAGT TCGCATCTGA ACGGGATGGG 
GACACGTTGC CAGACCGGAA GAATTTTGAC TCCCTGCTCT GGGCCATTGT CACTGTCTTT 
CAGATTCTGA CTCAGGAAGA CTGGAATAAA GTCCTTTACA ACGGCATGGC CTCCACGTCA 
TCTTGGGCTG CTCTTTACTT CATCGCCCTC ATGACTTTTG GCAACTACGT GCTCTTTAAC 
CTGCTTGTCG CCATTCTCGT GGAGGGTTTC CAGGCAGAGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAT CTAATGTTGG ACGATGTAAT TGCTTCCGGC 
AGCTCAGCCA GCGCTGCGTC AGAAGCCCAG TGCAAACCCT ACTACTCTGA CTACTCGCGC 
TTCCGGCTCC TCGTCCACCA CCTGTGTACC AGCCACTACC TGGACCTCTT CATCACTGGT 
GTCATCGGGC TGAATGTGGT CACGATGGCC ATGGAACATT ACCAGCAGCC CCAGATCCTG 
GACGAGGCTC TGAAGATCTG CAACTACATC TTTACCGTCA TCTTTGTCTT GGAGTCAGTA 
TTCAAACTTG TGGCCTTCGG CTTCCGCCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCTATTG TGCTTCTGTC CATCATGGGC ATCACGCTGG AAGAGATTGA GGTCAATGCT 
TCACTGCCCA TCAACCCCAC CATCATCCGT ATCATGAGGG TGCTCCGCAT TGCTCGAGTT 
CTGAAGCTGT TGAAGATGGC TGTGGGCATG CGGGCACTGC TGGACACGGT GATGCAGGCC 
CTGCCCCAGG TGGGGAACCT GGGACTTCTC TTCATGCTAT TATTTTTCAT CTTTGCAGCT 
CTGGGCGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CACACCCTTG TGAGGGCTTG 
GGCCGGCATG CCACCTTTAG GAACTTTGGT ATGGCCTTTC TGACCCTCTT CCGAGTCTCC 
ACTGGTGACA ACTGGAATGG TATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACCTGCTACA ACACCGTCAT CTCACCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT CATAGCCGTG CTGATGAAGC ACCTGGAAGA GAGCAACAAA 
GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG GAGCTGGAGC TAGAGATGAA GACACTCAGC 
CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT 
AGCCCTGACA GCCCTAAGCC TGGGGCTCCA CACACCACGG CCCACATTGG AGCAGCCTCT 
TCAGGCTTCT CCCTTGAGCA CCCCACGATG GTACCTCACA CTGAGGAGGG GCCAGTCCCC 
CTAGGACCAG ACCTGCTGAC TGTGAGGAAG TCTGGTGTCA GCCGGACACA CTCTCTGCCC 
AATGACAGCT ACATGTGCCG CAATGGGAGC ACTGCCGAGA GATCCCTAGG ACACAGGGGC 
TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC ATCTTGTCTG TTCACTCCCA ACCAGCAGAC 
ACCAGCTGCA TCCTACAGCT TCCCAAAGAT GCACACTATC TGCTCCAGCC TCATGGGGCT 
CCCACCTGGG GCGCCATCCC TAAACTACCC CCACCTGGCC GCTCCCCTCT GGCTCAGAGG 
CCTCTCAGGC GCCAGGCAGC AATAAGGACT GACTCCCTGG ACGTGCAGGG CCTGGGTAGC 
CGGGAAGACC TGTTGTCAGA GGTGAGTGGG CCCTCCTGCC CTCTGACCCG CTCCTCATCC 
TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC 
AAGCACATCC GCCTGCCAGC CCCTTGCCCA GGCCTGGAAC CCAGCTGGGC CAAGGACCCT 
CAAGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC CCACGGGACT TGAAAAAATG CTACAGTGTA 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG TCCTGGCTAG ACGAACAGAG GAGACACTCC 
ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC CAGCCCCGCC TATGTCCAAG CCCCTCAAGC 
CTCGGGGGCC AACCTCTTGG GGGCCCTGGG AGCCGGCCTA AGAAAAAACT CAGCCCACCC 
AGTATCTCTA TAGACCCCCC GGAGAGCCAG GGCCCTCGGC CCCCATGCAG TCCTGGCGTC 
TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC TCGAAGGATC CCTCGGCCTC CAGCCCCCTT 
GACAGCACGG CTGCCTCACC CTCCCCAAAG AAAGATGCGC TGAGTCTCTC TGGTTTGTCT 
TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171361.1
CDS	582..7328
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	NM_001177890.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177890.1

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
TTCTCTGAGA CCAAGCAACG GGAGAGTCAG CTGATGCGGG AGCAGCGTGT ACGATTCCTG 
TCCAATGCTA GCACCCTGGC AAGCTTCTCT GAGCCAGGCA GCTGCTATGA GGAGCTTCTC 
AAGTACCTGG TGTACATCCT CCGCAAAGCA GCCCGCAGGC TGGCCCAGGT CTCTAGGGCT 
GTAGGCGTGC GGGCTGGGTT GCTCAGCAGC CCAGTGGCCC GTGGTGGGCA GGAGCCCCAG 
CCCAGTGGCA GCTGCTCTCG TTCACACCGT CGTCTGTCTG TCCACCACCT GGTCCACCAC 
CATCACCACC ACCATCACCA CTACCACCTG GGTAATGGGA CGCTCAGAGT TCCCCGGGCC 
AGCCCAGAGA TCCAGGACAG GGATGCCAAT GGGTCCCGCT GGCTCATGCT GCCACCACCC 
TCTACCCCCA CTCCCTCTGG GGGCCCTCCG AGGGGTGCGG AGTCTGTACA CAGCTTCTAC 
CATGCTGACT GCCACTTGGA GCCAGTCCGT TGCCAGGCGC CCCCTCCCAG GTCCCCATCG 
GAGGCATCTG GCAGGACTGT GGGTAGTGGG AAGGTATACC CCACTGTGCA TACCAGCCCT 
CCACCAGAGA TGCTGAAGGA TAAGGCACTA GTGGAGGTGG CCCCCAGCCC TGGGCCCCCC 
ACCCTCACCA GCTTCAACAT CCCACCTGGG CCCTTCAGCT CCATGCACAA GCTCCTGGAG 
ACACAGAGTA CGGGAGCCTG CCATAGCTCC TGCAAAATCT CCAGCCCTTG CTCCAAGGCA 
GACAGTGGAG CCTGTGGGCC GGACAGTTGT CCCTACTGTG CCCGGACAGG AGCAGGGGAG 
CCAGAGTCCG CTGACCATGA AATGCCTGAC TCAGACAGTG AGGCTGTGTA TGAGTTCACA 
CAGGACGCTC AGCACAGCGA CCTCCGGGAT CCCCACAGAC GGCGACGGCC GAGCCTGGGC 
CCAGATGCAG AGCCTAGTTC TGTGCTGGCC TTCTGGAGGC TGATCTGCGA CACATTCCGG 
AAGATCGTAG ATAGCAAATA CTTTGGCCGG GGAATCATGA TCGCTATCCT GGTCAATACC 
CTCAGCATGG GCATCGAGTA CCACGAGCAG CCCGAGGAGC TCACCAACGC CCTGGAAATC 
AGCAACATCG TCTTCACCAG CCTCTTCGCC TTGGAGATGC TGCTGAAGCT GCTCGTCTAC 
GGTCCCTTCG GCTACATTAA GAATCCCTAC AACATCTTTG ATGGCGTCAT TGTGGTCATC 
AGCGTGTGGG AGATTGTGGG CCAGCAGGGA GGTGGCCTGT CGGTGCTGCG GACCTTCCGC 
CTGATGCGGG TGCTGAAGCT AGTTCGCTTC CTGCCGGCAC TGCAGCGGCA GCTCGTGGTG 
CTCATGAAGA CCATGGACAA CGTGGCCACC TTCTGCATGC TGCTCATGCT CTTCATCTTC 
ATCTTCAGCA TCCTGGGCAT GCACCTTTTT GGTTGCAAGT TCGCATCTGA ACGGGATGGG 
GACACGTTGC CAGACCGGAA GAATTTTGAC TCCCTGCTCT GGGCCATTGT CACTGTCTTT 
CAGATTCTGA CTCAGGAAGA CTGGAATAAA GTCCTTTACA ACGGCATGGC CTCCACGTCA 
TCTTGGGCTG CTCTTTACTT CATCGCCCTC ATGACTTTTG GCAACTACGT GCTCTTTAAC 
CTGCTTGTCG CCATTCTCGT GGAGGGTTTC CAGGCAGAGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAT CTAATGTTGG ACGATGTAAT TGCTTCCGGC 
AGCTCAGCCA GCGCTGCGTC AGAAGCCCAG TGCAAACCCT ACTACTCTGA CTACTCGCGC 
TTCCGGCTCC TCGTCCACCA CCTGTGTACC AGCCACTACC TGGACCTCTT CATCACTGGT 
GTCATCGGGC TGAATGTGGT CACGATGGCC ATGGAACATT ACCAGCAGCC CCAGATCCTG 
GACGAGGCTC TGAAGATCTG CAACTACATC TTTACCGTCA TCTTTGTCTT GGAGTCAGTA 
TTCAAACTTG TGGCCTTCGG CTTCCGCCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCTATTG TGCTTCTGTC CATCATGGGC ATCACGCTGG AAGAGATTGA GGTCAATGCT 
TCACTGCCCA TCAACCCCAC CATCATCCGT ATCATGAGGG TGCTCCGCAT TGCTCGAGTT 
CTGAAGCTGT TGAAGATGGC TGTGGGCATG CGGGCACTGC TGGACACGGT GATGCAGGCC 
CTGCCCCAGG TGGGGAACCT GGGACTTCTC TTCATGCTAT TATTTTTCAT CTTTGCAGCT 
CTGGGCGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CACACCCTTG TGAGGGCTTG 
GGCCGGCATG CCACCTTTAG GAACTTTGGT ATGGCCTTTC TGACCCTCTT CCGAGTCTCC 
ACTGGTGACA ACTGGAATGG TATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACCTGCTACA ACACCGTCAT CTCACCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT CATAGCCGTG CTGATGAAGC ACCTGGAAGA GAGCAACAAA 
GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG GAGCTGGAGC TAGAGATGAA GACACTCAGC 
CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT 
AGCCCTGACA GCCCTAAGCC TGGGGCTCCA CACACCACGG CCCACATTGG AGCAGCCTCT 
TCAGGCTTCT CCCTTGAGCA CCCCACGATG GTACCTCACA CTGAGGAGGG GCCAGTCCCC 
CTAGGACCAG ACCTGCTGAC TGTGAGGAAG TCTGGTGTCA GCCGGACACA CTCTCTGCCC 
AATGACAGCT ACATGTGCCG CAATGGGAGC ACTGCCGAGA GATCCCTAGG ACACAGGGGC 
TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC ATCTTGTCTG TTCACTCCCA ACCAGCAGAC 
ACCAGCTGCA TCCTACAGCT TCCCAAAGAT GCACACTATC TGCTCCAGCC TCATGGGGCT 
CCCACCTGGG GCGCCATCCC TAAACTACCC CCACCTGGCC GCTCCCCTCT GGCTCAGAGG 
CCTCTCAGGC GCCAGGCAGC AATAAGGACT GACTCCCTGG ACGTGCAGGG CCTGGGTAGC 
CGGGAAGACC TGTTGTCAGA GGTGAGTGGG CCCTCCTGCC CTCTGACCCG CTCCTCATCC 
TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC 
AAGCACATCC GCCTGCCAGC CCCTTGCCCA GGCCTGGAAC CCAGCTGGGC CAAGGACCCT 
CAAGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC CCACGGGACT TGAAAAAATG CTACAGTGTA 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG TCCTGGCTAG ACGAACAGAG GAGACACTCC 
ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC CAGCCCCGCC TATGTCCAAG CCCCTCAAGC 
CTCGGGGGCC AACCTCTTGG GGGCCCTGGG AGCCGGCCTA AGAAAAAACT CAGCCCACCC 
AGTATCTCTA TAGACCCCCC GGAGAGCCAG GGCCCTCGGC CCCCATGCAG TCCTGGCGTC 
TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC TCGAAGGATC CCTCGGCCTC CAGCCCCCTT 
GACAGCACGG CTGCCTCACC CTCCCCAAAG AAAGATGCGC TGAGTCTCTC TGGTTTGTCT 
TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( NM_001112813.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001106284.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant 2, mRNA.

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CloneID	OMu16069
Clone ID Related Accession (Same CDS sequence)	NM_001112813.2
Accession Version	NM_001112813.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF536661.1, AJ012569.1, DQ317412.1, AW124914.1 and AW457316.1. On May 1, 2010 this sequence version replaced NM_001112813.1. Transcript Variant: This variant (2) lacks an alternate, in-frame coding segment, compared to variant 1. The resulting protein (isoform b) is shorter when it is compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG ATCTAATGTT GGACGATGTA 
ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG TCAGAAGCCC AGTGCAAACC CTACTACTCT 
GACTACTCGC GCTTCCGGCT CCTCGTCCAC CACCTGTGTA CCAGCCACTA CCTGGACCTC 
TTCATCACTG GTGTCATCGG GCTGAATGTG GTCACGATGG CCATGGAACA TTACCAGCAG 
CCCCAGATCC TGGACGAGGC TCTGAAGATC TGCAACTACA TCTTTACCGT CATCTTTGTC 
TTGGAGTCAG TATTCAAACT TGTGGCCTTC GGCTTCCGCC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCTAT TGTGCTTCTG TCCATCATGG GCATCACGCT GGAAGAGATT 
GAGGTCAATG CTTCACTGCC CATCAACCCC ACCATCATCC GTATCATGAG GGTGCTCCGC 
ATTGCTCGAG TTCTGAAGCT GTTGAAGATG GCTGTGGGCA TGCGGGCACT GCTGGACACG 
GTGATGCAGG CCCTGCCCCA GGTGGGGAAC CTGGGACTTC TCTTCATGCT ATTATTTTTC 
ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACACACCCT 
TGTGAGGGCT TGGGCCGGCA TGCCACCTTT AGGAACTTTG GTATGGCCTT TCTGACCCTC 
TTCCGAGTCT CCACTGGTGA CAACTGGAAT GGTATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACCTGCTA CAACACCGTC ATCTCACCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTCATAGCCG TGCTGATGAA GCACCTGGAA 
GAGAGCAACA AAGAGGCCAA GGAGGAGGCG GAGTTGGAGG CGGAGCTGGA GCTAGAGATG 
AAGACACTCA GCCCGCAGCC CCACTCCCCG CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG 
GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG CCTGGGGCTC CACACACCAC GGCCCACATT 
GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG CACCCCACGA TGGTACCTCA CACTGAGGAG 
GGGCCAGTCC CCCTAGGACC AGACCTGCTG ACTGTGAGGA AGTCTGGTGT CAGCCGGACA 
CACTCTCTGC CCAATGACAG CTACATGTGC CGCAATGGGA GCACTGCCGA GAGATCCCTA 
GGACACAGGG GCTGGGGGCT CCCCAAAGCC CAGTCAGGCT CCATCTTGTC TGTTCACTCC 
CAACCAGCAG ACACCAGCTG CATCCTACAG CTTCCCAAAG ATGCACACTA TCTGCTCCAG 
CCTCATGGGG CTCCCACCTG GGGCGCCATC CCTAAACTAC CCCCACCTGG CCGCTCCCCT 
CTGGCTCAGA GGCCTCTCAG GCGCCAGGCA GCAATAAGGA CTGACTCCCT GGACGTGCAG 
GGCCTGGGTA GCCGGGAAGA CCTGTTGTCA GAGGTGAGTG GGCCCTCCTG CCCTCTGACC 
CGCTCCTCAT CCTTCTGGGG CGGGTCGAGC ATCCAGGTGC AGCAGCGCTC CGGCAGCCAG 
AGCAAAGTCT CCAAGCACAT CCGCCTGCCA GCCCCTTGCC CAGGCCTGGA ACCCAGCTGG 
GCCAAGGACC CTCAAGAGAC CAGAAGCAGC TTAGAGCTGG ACACGGAGCT GAGCTGGATT 
TCAGGAGACC TCCTGCCCAG CAGTCAGGAA GAACCCCTGT CCCCACGGGA CTTGAAAAAA 
TGCTACAGTG TAGAGGCCCA GAGCTGCCGG CGCAGGCCTG GGTCCTGGCT AGACGAACAG 
AGGAGACACT CCATCGCTGT CAGCTGCCTG GACAGCGGCT CCCAGCCCCG CCTATGTCCA 
AGCCCCTCAA GCCTCGGGGG CCAACCTCTT GGGGGCCCTG GGAGCCGGCC TAAGAAAAAA 
CTCAGCCCAC CCAGTATCTC TATAGACCCC CCGGAGAGCC AGGGCCCTCG GCCCCCATGC 
AGTCCTGGCG TCTGCCTCAG GAGGAGGGCG CCGGCCAGTG ACTCGAAGGA TCCCTCGGCC 
TCCAGCCCCC TTGACAGCAC GGCTGCCTCA CCCTCCCCAA AGAAAGATGC GCTGAGTCTC 
TCTGGTTTGT CTTCTGACCC AACAGACCTG GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106284.1
CDS	582..7400
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	NM_001112813.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112813.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG ATCTAATGTT GGACGATGTA 
ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG TCAGAAGCCC AGTGCAAACC CTACTACTCT 
GACTACTCGC GCTTCCGGCT CCTCGTCCAC CACCTGTGTA CCAGCCACTA CCTGGACCTC 
TTCATCACTG GTGTCATCGG GCTGAATGTG GTCACGATGG CCATGGAACA TTACCAGCAG 
CCCCAGATCC TGGACGAGGC TCTGAAGATC TGCAACTACA TCTTTACCGT CATCTTTGTC 
TTGGAGTCAG TATTCAAACT TGTGGCCTTC GGCTTCCGCC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCTAT TGTGCTTCTG TCCATCATGG GCATCACGCT GGAAGAGATT 
GAGGTCAATG CTTCACTGCC CATCAACCCC ACCATCATCC GTATCATGAG GGTGCTCCGC 
ATTGCTCGAG TTCTGAAGCT GTTGAAGATG GCTGTGGGCA TGCGGGCACT GCTGGACACG 
GTGATGCAGG CCCTGCCCCA GGTGGGGAAC CTGGGACTTC TCTTCATGCT ATTATTTTTC 
ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACACACCCT 
TGTGAGGGCT TGGGCCGGCA TGCCACCTTT AGGAACTTTG GTATGGCCTT TCTGACCCTC 
TTCCGAGTCT CCACTGGTGA CAACTGGAAT GGTATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACCTGCTA CAACACCGTC ATCTCACCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTCATAGCCG TGCTGATGAA GCACCTGGAA 
GAGAGCAACA AAGAGGCCAA GGAGGAGGCG GAGTTGGAGG CGGAGCTGGA GCTAGAGATG 
AAGACACTCA GCCCGCAGCC CCACTCCCCG CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG 
GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG CCTGGGGCTC CACACACCAC GGCCCACATT 
GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG CACCCCACGA TGGTACCTCA CACTGAGGAG 
GGGCCAGTCC CCCTAGGACC AGACCTGCTG ACTGTGAGGA AGTCTGGTGT CAGCCGGACA 
CACTCTCTGC CCAATGACAG CTACATGTGC CGCAATGGGA GCACTGCCGA GAGATCCCTA 
GGACACAGGG GCTGGGGGCT CCCCAAAGCC CAGTCAGGCT CCATCTTGTC TGTTCACTCC 
CAACCAGCAG ACACCAGCTG CATCCTACAG CTTCCCAAAG ATGCACACTA TCTGCTCCAG 
CCTCATGGGG CTCCCACCTG GGGCGCCATC CCTAAACTAC CCCCACCTGG CCGCTCCCCT 
CTGGCTCAGA GGCCTCTCAG GCGCCAGGCA GCAATAAGGA CTGACTCCCT GGACGTGCAG 
GGCCTGGGTA GCCGGGAAGA CCTGTTGTCA GAGGTGAGTG GGCCCTCCTG CCCTCTGACC 
CGCTCCTCAT CCTTCTGGGG CGGGTCGAGC ATCCAGGTGC AGCAGCGCTC CGGCAGCCAG 
AGCAAAGTCT CCAAGCACAT CCGCCTGCCA GCCCCTTGCC CAGGCCTGGA ACCCAGCTGG 
GCCAAGGACC CTCAAGAGAC CAGAAGCAGC TTAGAGCTGG ACACGGAGCT GAGCTGGATT 
TCAGGAGACC TCCTGCCCAG CAGTCAGGAA GAACCCCTGT CCCCACGGGA CTTGAAAAAA 
TGCTACAGTG TAGAGGCCCA GAGCTGCCGG CGCAGGCCTG GGTCCTGGCT AGACGAACAG 
AGGAGACACT CCATCGCTGT CAGCTGCCTG GACAGCGGCT CCCAGCCCCG CCTATGTCCA 
AGCCCCTCAA GCCTCGGGGG CCAACCTCTT GGGGGCCCTG GGAGCCGGCC TAAGAAAAAA 
CTCAGCCCAC CCAGTATCTC TATAGACCCC CCGGAGAGCC AGGGCCCTCG GCCCCCATGC 
AGTCCTGGCG TCTGCCTCAG GAGGAGGGCG CCGGCCAGTG ACTCGAAGGA TCCCTCGGCC 
TCCAGCCCCC TTGACAGCAC GGCTGCCTCA CCCTCCCCAA AGAAAGATGC GCTGAGTCTC 
TCTGGTTTGT CTTCTGACCC AACAGACCTG GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( NM_009783.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033913.2 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant 1, mRNA.

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CloneID	OMu16165
Clone ID Related Accession (Same CDS sequence)	NM_009783.3
Accession Version	NM_009783.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF536661.1, AJ012569.1, DQ317412.1, AW124914.1 and AW457316.1. On May 1, 2010 this sequence version replaced NM_009783.2. Transcript Variant: This transcript (1) represents the longest transcript and it encodes the longest protein (isoform a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ012569.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA TCTAATGTTG 
GACGATGTAA TTGCTTCCGG CAGCTCAGCC AGCGCTGCGT CAGAAGCCCA GTGCAAACCC 
TACTACTCTG ACTACTCGCG CTTCCGGCTC CTCGTCCACC ACCTGTGTAC CAGCCACTAC 
CTGGACCTCT TCATCACTGG TGTCATCGGG CTGAATGTGG TCACGATGGC CATGGAACAT 
TACCAGCAGC CCCAGATCCT GGACGAGGCT CTGAAGATCT GCAACTACAT CTTTACCGTC 
ATCTTTGTCT TGGAGTCAGT ATTCAAACTT GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCTATT GTGCTTCTGT CCATCATGGG CATCACGCTG 
GAAGAGATTG AGGTCAATGC TTCACTGCCC ATCAACCCCA CCATCATCCG TATCATGAGG 
GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG TTGAAGATGG CTGTGGGCAT GCGGGCACTG 
CTGGACACGG TGATGCAGGC CCTGCCCCAG GTGGGGAACC TGGGACTTCT CTTCATGCTA 
TTATTTTTCA TCTTTGCAGC TCTGGGCGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACACACCCTT GTGAGGGCTT GGGCCGGCAT GCCACCTTTA GGAACTTTGG TATGGCCTTT 
CTGACCCTCT TCCGAGTCTC CACTGGTGAC AACTGGAATG GTATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACCTGCTAC AACACCGTCA TCTCACCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TCATAGCCGT GCTGATGAAG 
CACCTGGAAG AGAGCAACAA AGAGGCCAAG GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG 
CTAGAGATGA AGACACTCAG CCCGCAGCCC CACTCCCCGC TGGGCAGCCC CTTCCTCTGG 
CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC AGCCCTAAGC CTGGGGCTCC ACACACCACG 
GCCCACATTG GAGCAGCCTC TTCAGGCTTC TCCCTTGAGC ACCCCACGAT GGTACCTCAC 
ACTGAGGAGG GGCCAGTCCC CCTAGGACCA GACCTGCTGA CTGTGAGGAA GTCTGGTGTC 
AGCCGGACAC ACTCTCTGCC CAATGACAGC TACATGTGCC GCAATGGGAG CACTGCCGAG 
AGATCCCTAG GACACAGGGG CTGGGGGCTC CCCAAAGCCC AGTCAGGCTC CATCTTGTCT 
GTTCACTCCC AACCAGCAGA CACCAGCTGC ATCCTACAGC TTCCCAAAGA TGCACACTAT 
CTGCTCCAGC CTCATGGGGC TCCCACCTGG GGCGCCATCC CTAAACTACC CCCACCTGGC 
CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG CGCCAGGCAG CAATAAGGAC TGACTCCCTG 
GACGTGCAGG GCCTGGGTAG CCGGGAAGAC CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC 
CCTCTGACCC GCTCCTCATC CTTCTGGGGC GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC 
GGCAGCCAGA GCAAAGTCTC CAAGCACATC CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA 
CCCAGCTGGG CCAAGGACCC TCAAGAGACC AGAAGCAGCT TAGAGCTGGA CACGGAGCTG 
AGCTGGATTT CAGGAGACCT CCTGCCCAGC AGTCAGGAAG AACCCCTGTC CCCACGGGAC 
TTGAAAAAAT GCTACAGTGT AGAGGCCCAG AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA 
GACGAACAGA GGAGACACTC CATCGCTGTC AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC 
CTATGTCCAA GCCCCTCAAG CCTCGGGGGC CAACCTCTTG GGGGCCCTGG GAGCCGGCCT 
AAGAAAAAAC TCAGCCCACC CAGTATCTCT ATAGACCCCC CGGAGAGCCA GGGCCCTCGG 
CCCCCATGCA GTCCTGGCGT CTGCCTCAGG AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT 
CCCTCGGCCT CCAGCCCCCT TGACAGCACG GCTGCCTCAC CCTCCCCAAA GAAAGATGCG 
CTGAGTCTCT CTGGTTTGTC TTCTGACCCA ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033913.2
CDS	582..7469
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	NM_009783.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009783.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA TCTAATGTTG 
GACGATGTAA TTGCTTCCGG CAGCTCAGCC AGCGCTGCGT CAGAAGCCCA GTGCAAACCC 
TACTACTCTG ACTACTCGCG CTTCCGGCTC CTCGTCCACC ACCTGTGTAC CAGCCACTAC 
CTGGACCTCT TCATCACTGG TGTCATCGGG CTGAATGTGG TCACGATGGC CATGGAACAT 
TACCAGCAGC CCCAGATCCT GGACGAGGCT CTGAAGATCT GCAACTACAT CTTTACCGTC 
ATCTTTGTCT TGGAGTCAGT ATTCAAACTT GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCTATT GTGCTTCTGT CCATCATGGG CATCACGCTG 
GAAGAGATTG AGGTCAATGC TTCACTGCCC ATCAACCCCA CCATCATCCG TATCATGAGG 
GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG TTGAAGATGG CTGTGGGCAT GCGGGCACTG 
CTGGACACGG TGATGCAGGC CCTGCCCCAG GTGGGGAACC TGGGACTTCT CTTCATGCTA 
TTATTTTTCA TCTTTGCAGC TCTGGGCGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACACACCCTT GTGAGGGCTT GGGCCGGCAT GCCACCTTTA GGAACTTTGG TATGGCCTTT 
CTGACCCTCT TCCGAGTCTC CACTGGTGAC AACTGGAATG GTATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACCTGCTAC AACACCGTCA TCTCACCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TCATAGCCGT GCTGATGAAG 
CACCTGGAAG AGAGCAACAA AGAGGCCAAG GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG 
CTAGAGATGA AGACACTCAG CCCGCAGCCC CACTCCCCGC TGGGCAGCCC CTTCCTCTGG 
CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC AGCCCTAAGC CTGGGGCTCC ACACACCACG 
GCCCACATTG GAGCAGCCTC TTCAGGCTTC TCCCTTGAGC ACCCCACGAT GGTACCTCAC 
ACTGAGGAGG GGCCAGTCCC CCTAGGACCA GACCTGCTGA CTGTGAGGAA GTCTGGTGTC 
AGCCGGACAC ACTCTCTGCC CAATGACAGC TACATGTGCC GCAATGGGAG CACTGCCGAG 
AGATCCCTAG GACACAGGGG CTGGGGGCTC CCCAAAGCCC AGTCAGGCTC CATCTTGTCT 
GTTCACTCCC AACCAGCAGA CACCAGCTGC ATCCTACAGC TTCCCAAAGA TGCACACTAT 
CTGCTCCAGC CTCATGGGGC TCCCACCTGG GGCGCCATCC CTAAACTACC CCCACCTGGC 
CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG CGCCAGGCAG CAATAAGGAC TGACTCCCTG 
GACGTGCAGG GCCTGGGTAG CCGGGAAGAC CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC 
CCTCTGACCC GCTCCTCATC CTTCTGGGGC GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC 
GGCAGCCAGA GCAAAGTCTC CAAGCACATC CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA 
CCCAGCTGGG CCAAGGACCC TCAAGAGACC AGAAGCAGCT TAGAGCTGGA CACGGAGCTG 
AGCTGGATTT CAGGAGACCT CCTGCCCAGC AGTCAGGAAG AACCCCTGTC CCCACGGGAC 
TTGAAAAAAT GCTACAGTGT AGAGGCCCAG AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA 
GACGAACAGA GGAGACACTC CATCGCTGTC AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC 
CTATGTCCAA GCCCCTCAAG CCTCGGGGGC CAACCTCTTG GGGGCCCTGG GAGCCGGCCT 
AAGAAAAAAC TCAGCCCACC CAGTATCTCT ATAGACCCCC CGGAGAGCCA GGGCCCTCGG 
CCCCCATGCA GTCCTGGCGT CTGCCTCAGG AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT 
CCCTCGGCCT CCAGCCCCCT TGACAGCACG GCTGCCTCAC CCTCCCCAAA GAAAGATGCG 
CTGAGTCTCT CTGGTTTGTC TTCTGACCCA ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( NM_001177888.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171359.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant 3, mRNA.

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CloneID	OMu16231
Clone ID Related Accession (Same CDS sequence)	NM_001177888.1
Accession Version	NM_001177888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645965.8, BC057399.1, AJ012569.1 and AW457316.1. Transcript Variant: This variant (3) lacks an alternate, in-frame coding segment and uses a different splice site in the 3' coding region, compared to variant 1. The resulting protein (isoform b) is shorter when it is compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ317412.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAA TCTAATGTTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCTGCGT CAGAAGCCCA GTGCAAACCC TACTACTCTG ACTACTCGCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGACCTCT TCATCACTGG TGTCATCGGG 
CTGAATGTGG TCACGATGGC CATGGAACAT TACCAGCAGC CCCAGATCCT GGACGAGGCT 
CTGAAGATCT GCAACTACAT CTTTACCGTC ATCTTTGTCT TGGAGTCAGT ATTCAAACTT 
GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCTATT 
GTGCTTCTGT CCATCATGGG CATCACGCTG GAAGAGATTG AGGTCAATGC TTCACTGCCC 
ATCAACCCCA CCATCATCCG TATCATGAGG GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG 
TTGAAGATGG CTGTGGGCAT GCGGGCACTG CTGGACACGG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACC TGGGACTTCT CTTCATGCTA TTATTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGACCTGGA GTGTGATGAG ACACACCCTT GTGAGGGCTT GGGCCGGCAT 
GCCACCTTTA GGAACTTTGG TATGGCCTTT CTGACCCTCT TCCGAGTCTC CACTGGTGAC 
AACTGGAATG GTATTATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACCGTCA TCTCACCCAT CTACTTCGTG TCCTTCGTGC TGACGGCCCA GTTTGTGCTG 
GTCAACGTGG TCATAGCCGT GCTGATGAAG CACCTGGAAG AGAGCAACAA AGAGGCCAAG 
GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG CTAGAGATGA AGACACTCAG CCCGCAGCCC 
CACTCCCCGC TGGGCAGCCC CTTCCTCTGG CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC 
AGCCCTAAGC CTGGGGCTCC ACACACCACG GCCCACATTG GAGCAGCCTC TTCAGGCTTC 
TCCCTTGAGC ACCCCACGAT GGTACCTCAC ACTGAGGAGG GGCCAGTCCC CCTAGGACCA 
GACCTGCTGA CTGTGAGGAA GTCTGGTGTC AGCCGGACAC ACTCTCTGCC CAATGACAGC 
TACATGTGCC GCAATGGGAG CACTGCCGAG AGATCCCTAG GACACAGGGG CTGGGGGCTC 
CCCAAAGCCC AGTCAGGCTC CATCTTGTCT GTTCACTCCC AACCAGCAGA CACCAGCTGC 
ATCCTACAGC TTCCCAAAGA TGCACACTAT CTGCTCCAGC CTCATGGGGC TCCCACCTGG 
GGCGCCATCC CTAAACTACC CCCACCTGGC CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG 
CGCCAGGCAG CAATAAGGAC TGACTCCCTG GACGTGCAGG GCCTGGGTAG CCGGGAAGAC 
CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC CCTCTGACCC GCTCCTCATC CTTCTGGGGC 
GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC GGCAGCCAGA GCAAAGTCTC CAAGCACATC 
CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA CCCAGCTGGG CCAAGGACCC TCAAGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGCCCAGC 
AGTCAGGAAG AACCCCTGTC CCCACGGGAC TTGAAAAAAT GCTACAGTGT AGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA GACGAACAGA GGAGACACTC CATCGCTGTC 
AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC CTATGTCCAA GCCCCTCAAG CCTCGGGGGC 
CAACCTCTTG GGGGCCCTGG GAGCCGGCCT AAGAAAAAAC TCAGCCCACC CAGTATCTCT 
ATAGACCCCC CGGAGAGCCA GGGCCCTCGG CCCCCATGCA GTCCTGGCGT CTGCCTCAGG 
AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT CCCTCGGCCT CCAGCCCCCT TGACAGCACG 
GCTGCCTCAC CCTCCCCAAA GAAAGATGCG CTGAGTCTCT CTGGTTTGTC TTCTGACCCA 
ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171359.1
CDS	582..7379
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	NM_001177888.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177888.1

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAA TCTAATGTTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCTGCGT CAGAAGCCCA GTGCAAACCC TACTACTCTG ACTACTCGCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGACCTCT TCATCACTGG TGTCATCGGG 
CTGAATGTGG TCACGATGGC CATGGAACAT TACCAGCAGC CCCAGATCCT GGACGAGGCT 
CTGAAGATCT GCAACTACAT CTTTACCGTC ATCTTTGTCT TGGAGTCAGT ATTCAAACTT 
GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCTATT 
GTGCTTCTGT CCATCATGGG CATCACGCTG GAAGAGATTG AGGTCAATGC TTCACTGCCC 
ATCAACCCCA CCATCATCCG TATCATGAGG GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG 
TTGAAGATGG CTGTGGGCAT GCGGGCACTG CTGGACACGG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACC TGGGACTTCT CTTCATGCTA TTATTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGACCTGGA GTGTGATGAG ACACACCCTT GTGAGGGCTT GGGCCGGCAT 
GCCACCTTTA GGAACTTTGG TATGGCCTTT CTGACCCTCT TCCGAGTCTC CACTGGTGAC 
AACTGGAATG GTATTATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACCGTCA TCTCACCCAT CTACTTCGTG TCCTTCGTGC TGACGGCCCA GTTTGTGCTG 
GTCAACGTGG TCATAGCCGT GCTGATGAAG CACCTGGAAG AGAGCAACAA AGAGGCCAAG 
GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG CTAGAGATGA AGACACTCAG CCCGCAGCCC 
CACTCCCCGC TGGGCAGCCC CTTCCTCTGG CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC 
AGCCCTAAGC CTGGGGCTCC ACACACCACG GCCCACATTG GAGCAGCCTC TTCAGGCTTC 
TCCCTTGAGC ACCCCACGAT GGTACCTCAC ACTGAGGAGG GGCCAGTCCC CCTAGGACCA 
GACCTGCTGA CTGTGAGGAA GTCTGGTGTC AGCCGGACAC ACTCTCTGCC CAATGACAGC 
TACATGTGCC GCAATGGGAG CACTGCCGAG AGATCCCTAG GACACAGGGG CTGGGGGCTC 
CCCAAAGCCC AGTCAGGCTC CATCTTGTCT GTTCACTCCC AACCAGCAGA CACCAGCTGC 
ATCCTACAGC TTCCCAAAGA TGCACACTAT CTGCTCCAGC CTCATGGGGC TCCCACCTGG 
GGCGCCATCC CTAAACTACC CCCACCTGGC CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG 
CGCCAGGCAG CAATAAGGAC TGACTCCCTG GACGTGCAGG GCCTGGGTAG CCGGGAAGAC 
CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC CCTCTGACCC GCTCCTCATC CTTCTGGGGC 
GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC GGCAGCCAGA GCAAAGTCTC CAAGCACATC 
CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA CCCAGCTGGG CCAAGGACCC TCAAGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGCCCAGC 
AGTCAGGAAG AACCCCTGTC CCCACGGGAC TTGAAAAAAT GCTACAGTGT AGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA GACGAACAGA GGAGACACTC CATCGCTGTC 
AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC CTATGTCCAA GCCCCTCAAG CCTCGGGGGC 
CAACCTCTTG GGGGCCCTGG GAGCCGGCCT AAGAAAAAAC TCAGCCCACC CAGTATCTCT 
ATAGACCCCC CGGAGAGCCA GGGCCCTCGG CCCCCATGCA GTCCTGGCGT CTGCCTCAGG 
AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT CCCTCGGCCT CCAGCCCCCT TGACAGCACG 
GCTGCCTCAC CCTCCCCAAA GAAAGATGCG CTGAGTCTCT CTGGTTTGTC TTCTGACCCA 
ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532069.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532132.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X2, mRNA.

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CloneID	OMu45464
Clone ID Related Accession (Same CDS sequence)	XM_006532069.3 , XM_006532069.4
Accession Version	XM_006532069.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532069.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA TCTAATGTTG 
GACGATGTAA TTGCTTCCGG CAGCTCAGCC AGCGCTGCGT CAGAAGCCCA GTGCAAACCC 
TACTACTCTG ACTACTCGCG CTTCCGGCTC CTCGTCCACC ACCTGTGTAC CAGCCACTAC 
CTGGACCTCT TCATCACTGG TGTCATCGGG CTGAATGTGG TCACGATGGC CATGGAACAT 
TACCAGCAGC CCCAGATCCT GGACGAGGCT CTGAAGATCT GCAACTACAT CTTTACCGTC 
ATCTTTGTCT TGGAGTCAGT ATTCAAACTT GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCTATT GTGCTTCTGT CCATCATGGG CATCACGCTG 
GAAGAGATTG AGGTCAATGC TTCACTGCCC ATCAACCCCA CCATCATCCG TATCATGAGG 
GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG TTGAAGATGG CTGTGGGCAT GCGGGCACTG 
CTGGACACGG TGATGCAGGC CCTGCCCCAG GTGGGGAACC TGGGACTTCT CTTCATGCTA 
TTATTTTTCA TCTTTGCAGC TCTGGGCGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACACACCCTT GTGAGGGCTT GGGCCGGCAT GCCACCTTTA GGAACTTTGG TATGGCCTTT 
CTGACCCTCT TCCGAGTCTC CACTGGTGAC AACTGGAATG GTATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACCTGCTAC AACACCGTCA TCTCACCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TCATAGCCGT GCTGATGAAG 
CACCTGGAAG AGAGCAACAA AGAGGCCAAG GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG 
CTAGAGATGA AGACACTCAG CCCGCAGCCC CACTCCCCGC TGGGCAGCCC CTTCCTCTGG 
CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC AGCCCTAAGC CTGGGGCTCC ACACACCACG 
GCCCACATTG GAGCAGCCTC TTCAGGCTTC TCCCTTGAGC ACCCCACGGA CAGACAGCTG 
TTTGACACCA TCTCCCTGCT GATCCAGGGC TCCCTGGAGG GGGAGCTGAA GCTGATGGAC 
GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT GCCTTCCCTT CCGCCCCCAG CCCGGGCGAC 
TCCGACCCAC AGATCCCTCT AGCTGAGATG GAGGCTCTGT CTCTGACGTC AGAGATTGTG 
TCTGAACCGT CCTGCTCTCT AGCTCTGACG GATGACTCTT TGCCTGATGA CACTCACACA 
CTCTTACTTA GTGCCCTGGA GAGCAATATG GTACCTCACA CTGAGGAGGG GCCAGTCCCC 
CTAGGACCAG ACCTGCTGAC TGTGAGGAAG TCTGGTGTCA GCCGGACACA CTCTCTGCCC 
AATGACAGCT ACATGTGCCG CAATGGGAGC ACTGCCGAGA GATCCCTAGG ACACAGGGGC 
TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC ATCTTGTCTG TTCACTCCCA ACCAGCAGAC 
ACCAGCTGCA TCCTACAGCT TCCCAAAGAT GCACACTATC TGCTCCAGCC TCATGGGGCT 
CCCACCTGGG GCGCCATCCC TAAACTACCC CCACCTGGCC GCTCCCCTCT GGCTCAGAGG 
CCTCTCAGGC GCCAGGCAGC AATAAGGACT GACTCCCTGG ACGTGCAGGG CCTGGGTAGC 
CGGGAAGACC TGTTGTCAGA GGTGAGTGGG CCCTCCTGCC CTCTGACCCG CTCCTCATCC 
TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC 
AAGCACATCC GCCTGCCAGC CCCTTGCCCA GGCCTGGAAC CCAGCTGGGC CAAGGACCCT 
CAAGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC CCACGGGACT TGAAAAAATG CTACAGTGTA 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG TCCTGGCTAG ACGAACAGAG GAGACACTCC 
ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC CAGCCCCGCC TATGTCCAAG CCCCTCAAGC 
CTCGGGGGCC AACCTCTTGG GGGCCCTGGG AGCCGGCCTA AGAAAAAACT CAGCCCACCC 
AGTATCTCTA TAGACCCCCC GGAGAGCCAG GGCCCTCGGC CCCCATGCAG TCCTGGCGTC 
TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC TCGAAGGATC CCTCGGCCTC CAGCCCCCTT 
GACAGCACGG CTGCCTCACC CTCCCCAAAG AAAGATGCGC TGAGTCTCTC TGGTTTGTCT 
TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532132.1
CDS	773..7939
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532069.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532069.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA TCTAATGTTG 
GACGATGTAA TTGCTTCCGG CAGCTCAGCC AGCGCTGCGT CAGAAGCCCA GTGCAAACCC 
TACTACTCTG ACTACTCGCG CTTCCGGCTC CTCGTCCACC ACCTGTGTAC CAGCCACTAC 
CTGGACCTCT TCATCACTGG TGTCATCGGG CTGAATGTGG TCACGATGGC CATGGAACAT 
TACCAGCAGC CCCAGATCCT GGACGAGGCT CTGAAGATCT GCAACTACAT CTTTACCGTC 
ATCTTTGTCT TGGAGTCAGT ATTCAAACTT GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG 
GACAGGTGGA ACCAGCTGGA CCTGGCTATT GTGCTTCTGT CCATCATGGG CATCACGCTG 
GAAGAGATTG AGGTCAATGC TTCACTGCCC ATCAACCCCA CCATCATCCG TATCATGAGG 
GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG TTGAAGATGG CTGTGGGCAT GCGGGCACTG 
CTGGACACGG TGATGCAGGC CCTGCCCCAG GTGGGGAACC TGGGACTTCT CTTCATGCTA 
TTATTTTTCA TCTTTGCAGC TCTGGGCGTG GAGCTCTTTG GAGACCTGGA GTGTGATGAG 
ACACACCCTT GTGAGGGCTT GGGCCGGCAT GCCACCTTTA GGAACTTTGG TATGGCCTTT 
CTGACCCTCT TCCGAGTCTC CACTGGTGAC AACTGGAATG GTATTATGAA GGACACCCTC 
CGGGACTGTG ACCAGGAGTC CACCTGCTAC AACACCGTCA TCTCACCCAT CTACTTCGTG 
TCCTTCGTGC TGACGGCCCA GTTTGTGCTG GTCAACGTGG TCATAGCCGT GCTGATGAAG 
CACCTGGAAG AGAGCAACAA AGAGGCCAAG GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG 
CTAGAGATGA AGACACTCAG CCCGCAGCCC CACTCCCCGC TGGGCAGCCC CTTCCTCTGG 
CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC AGCCCTAAGC CTGGGGCTCC ACACACCACG 
GCCCACATTG GAGCAGCCTC TTCAGGCTTC TCCCTTGAGC ACCCCACGGA CAGACAGCTG 
TTTGACACCA TCTCCCTGCT GATCCAGGGC TCCCTGGAGG GGGAGCTGAA GCTGATGGAC 
GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT GCCTTCCCTT CCGCCCCCAG CCCGGGCGAC 
TCCGACCCAC AGATCCCTCT AGCTGAGATG GAGGCTCTGT CTCTGACGTC AGAGATTGTG 
TCTGAACCGT CCTGCTCTCT AGCTCTGACG GATGACTCTT TGCCTGATGA CACTCACACA 
CTCTTACTTA GTGCCCTGGA GAGCAATATG GTACCTCACA CTGAGGAGGG GCCAGTCCCC 
CTAGGACCAG ACCTGCTGAC TGTGAGGAAG TCTGGTGTCA GCCGGACACA CTCTCTGCCC 
AATGACAGCT ACATGTGCCG CAATGGGAGC ACTGCCGAGA GATCCCTAGG ACACAGGGGC 
TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC ATCTTGTCTG TTCACTCCCA ACCAGCAGAC 
ACCAGCTGCA TCCTACAGCT TCCCAAAGAT GCACACTATC TGCTCCAGCC TCATGGGGCT 
CCCACCTGGG GCGCCATCCC TAAACTACCC CCACCTGGCC GCTCCCCTCT GGCTCAGAGG 
CCTCTCAGGC GCCAGGCAGC AATAAGGACT GACTCCCTGG ACGTGCAGGG CCTGGGTAGC 
CGGGAAGACC TGTTGTCAGA GGTGAGTGGG CCCTCCTGCC CTCTGACCCG CTCCTCATCC 
TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC 
AAGCACATCC GCCTGCCAGC CCCTTGCCCA GGCCTGGAAC CCAGCTGGGC CAAGGACCCT 
CAAGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC CCACGGGACT TGAAAAAATG CTACAGTGTA 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG TCCTGGCTAG ACGAACAGAG GAGACACTCC 
ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC CAGCCCCGCC TATGTCCAAG CCCCTCAAGC 
CTCGGGGGCC AACCTCTTGG GGGCCCTGGG AGCCGGCCTA AGAAAAAACT CAGCCCACCC 
AGTATCTCTA TAGACCCCCC GGAGAGCCAG GGCCCTCGGC CCCCATGCAG TCCTGGCGTC 
TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC TCGAAGGATC CCTCGGCCTC CAGCCCCCTT 
GACAGCACGG CTGCCTCACC CTCCCCAAAG AAAGATGCGC TGAGTCTCTC TGGTTTGTCT 
TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532070.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532133.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X4, mRNA.

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CloneID	OMu45465
Clone ID Related Accession (Same CDS sequence)	XM_006532070.3 , XM_006532070.4
Accession Version	XM_006532070.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532070.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAT CTAATGTTGG ACGATGTAAT TGCTTCCGGC 
AGCTCAGCCA GCGCTGCGTC AGAAGCCCAG TGCAAACCCT ACTACTCTGA CTACTCGCGC 
TTCCGGCTCC TCGTCCACCA CCTGTGTACC AGCCACTACC TGGACCTCTT CATCACTGGT 
GTCATCGGGC TGAATGTGGT CACGATGGCC ATGGAACATT ACCAGCAGCC CCAGATCCTG 
GACGAGGCTC TGAAGATCTG CAACTACATC TTTACCGTCA TCTTTGTCTT GGAGTCAGTA 
TTCAAACTTG TGGCCTTCGG CTTCCGCCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCTATTG TGCTTCTGTC CATCATGGGC ATCACGCTGG AAGAGATTGA GGTCAATGCT 
TCACTGCCCA TCAACCCCAC CATCATCCGT ATCATGAGGG TGCTCCGCAT TGCTCGAGTT 
CTGAAGCTGT TGAAGATGGC TGTGGGCATG CGGGCACTGC TGGACACGGT GATGCAGGCC 
CTGCCCCAGG TGGGGAACCT GGGACTTCTC TTCATGCTAT TATTTTTCAT CTTTGCAGCT 
CTGGGCGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CACACCCTTG TGAGGGCTTG 
GGCCGGCATG CCACCTTTAG GAACTTTGGT ATGGCCTTTC TGACCCTCTT CCGAGTCTCC 
ACTGGTGACA ACTGGAATGG TATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACCTGCTACA ACACCGTCAT CTCACCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT CATAGCCGTG CTGATGAAGC ACCTGGAAGA GAGCAACAAA 
GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG GAGCTGGAGC TAGAGATGAA GACACTCAGC 
CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT 
AGCCCTGACA GCCCTAAGCC TGGGGCTCCA CACACCACGG CCCACATTGG AGCAGCCTCT 
TCAGGCTTCT CCCTTGAGCA CCCCACGGAC AGACAGCTGT TTGACACCAT CTCCCTGCTG 
ATCCAGGGCT CCCTGGAGGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTTC CGCCCCCAGC CCGGGCGACT CCGACCCACA GATCCCTCTA 
GCTGAGATGG AGGCTCTGTC TCTGACGTCA GAGATTGTGT CTGAACCGTC CTGCTCTCTA 
GCTCTGACGG ATGACTCTTT GCCTGATGAC ACTCACACAC TCTTACTTAG TGCCCTGGAG 
AGCAATATGG TACCTCACAC TGAGGAGGGG CCAGTCCCCC TAGGACCAGA CCTGCTGACT 
GTGAGGAAGT CTGGTGTCAG CCGGACACAC TCTCTGCCCA ATGACAGCTA CATGTGCCGC 
AATGGGAGCA CTGCCGAGAG ATCCCTAGGA CACAGGGGCT GGGGGCTCCC CAAAGCCCAG 
TCAGGCTCCA TCTTGTCTGT TCACTCCCAA CCAGCAGACA CCAGCTGCAT CCTACAGCTT 
CCCAAAGATG CACACTATCT GCTCCAGCCT CATGGGGCTC CCACCTGGGG CGCCATCCCT 
AAACTACCCC CACCTGGCCG CTCCCCTCTG GCTCAGAGGC CTCTCAGGCG CCAGGCAGCA 
ATAAGGACTG ACTCCCTGGA CGTGCAGGGC CTGGGTAGCC GGGAAGACCT GTTGTCAGAG 
GTGAGTGGGC CCTCCTGCCC TCTGACCCGC TCCTCATCCT TCTGGGGCGG GTCGAGCATC 
CAGGTGCAGC AGCGCTCCGG CAGCCAGAGC AAAGTCTCCA AGCACATCCG CCTGCCAGCC 
CCTTGCCCAG GCCTGGAACC CAGCTGGGCC AAGGACCCTC AAGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCAGCAG TCAGGAAGAA 
CCCCTGTCCC CACGGGACTT GAAAAAATGC TACAGTGTAG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGGGT CCTGGCTAGA CGAACAGAGG AGACACTCCA TCGCTGTCAG CTGCCTGGAC 
AGCGGCTCCC AGCCCCGCCT ATGTCCAAGC CCCTCAAGCC TCGGGGGCCA ACCTCTTGGG 
GGCCCTGGGA GCCGGCCTAA GAAAAAACTC AGCCCACCCA GTATCTCTAT AGACCCCCCG 
GAGAGCCAGG GCCCTCGGCC CCCATGCAGT CCTGGCGTCT GCCTCAGGAG GAGGGCGCCG 
GCCAGTGACT CGAAGGATCC CTCGGCCTCC AGCCCCCTTG ACAGCACGGC TGCCTCACCC 
TCCCCAAAGA AAGATGCGCT GAGTCTCTCT GGTTTGTCTT CTGACCCAAC AGACCTGGAT 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532133.1
CDS	773..7918
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532070.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532070.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAT CTAATGTTGG ACGATGTAAT TGCTTCCGGC 
AGCTCAGCCA GCGCTGCGTC AGAAGCCCAG TGCAAACCCT ACTACTCTGA CTACTCGCGC 
TTCCGGCTCC TCGTCCACCA CCTGTGTACC AGCCACTACC TGGACCTCTT CATCACTGGT 
GTCATCGGGC TGAATGTGGT CACGATGGCC ATGGAACATT ACCAGCAGCC CCAGATCCTG 
GACGAGGCTC TGAAGATCTG CAACTACATC TTTACCGTCA TCTTTGTCTT GGAGTCAGTA 
TTCAAACTTG TGGCCTTCGG CTTCCGCCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCTATTG TGCTTCTGTC CATCATGGGC ATCACGCTGG AAGAGATTGA GGTCAATGCT 
TCACTGCCCA TCAACCCCAC CATCATCCGT ATCATGAGGG TGCTCCGCAT TGCTCGAGTT 
CTGAAGCTGT TGAAGATGGC TGTGGGCATG CGGGCACTGC TGGACACGGT GATGCAGGCC 
CTGCCCCAGG TGGGGAACCT GGGACTTCTC TTCATGCTAT TATTTTTCAT CTTTGCAGCT 
CTGGGCGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CACACCCTTG TGAGGGCTTG 
GGCCGGCATG CCACCTTTAG GAACTTTGGT ATGGCCTTTC TGACCCTCTT CCGAGTCTCC 
ACTGGTGACA ACTGGAATGG TATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACCTGCTACA ACACCGTCAT CTCACCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT CATAGCCGTG CTGATGAAGC ACCTGGAAGA GAGCAACAAA 
GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG GAGCTGGAGC TAGAGATGAA GACACTCAGC 
CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT 
AGCCCTGACA GCCCTAAGCC TGGGGCTCCA CACACCACGG CCCACATTGG AGCAGCCTCT 
TCAGGCTTCT CCCTTGAGCA CCCCACGGAC AGACAGCTGT TTGACACCAT CTCCCTGCTG 
ATCCAGGGCT CCCTGGAGGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTTC CGCCCCCAGC CCGGGCGACT CCGACCCACA GATCCCTCTA 
GCTGAGATGG AGGCTCTGTC TCTGACGTCA GAGATTGTGT CTGAACCGTC CTGCTCTCTA 
GCTCTGACGG ATGACTCTTT GCCTGATGAC ACTCACACAC TCTTACTTAG TGCCCTGGAG 
AGCAATATGG TACCTCACAC TGAGGAGGGG CCAGTCCCCC TAGGACCAGA CCTGCTGACT 
GTGAGGAAGT CTGGTGTCAG CCGGACACAC TCTCTGCCCA ATGACAGCTA CATGTGCCGC 
AATGGGAGCA CTGCCGAGAG ATCCCTAGGA CACAGGGGCT GGGGGCTCCC CAAAGCCCAG 
TCAGGCTCCA TCTTGTCTGT TCACTCCCAA CCAGCAGACA CCAGCTGCAT CCTACAGCTT 
CCCAAAGATG CACACTATCT GCTCCAGCCT CATGGGGCTC CCACCTGGGG CGCCATCCCT 
AAACTACCCC CACCTGGCCG CTCCCCTCTG GCTCAGAGGC CTCTCAGGCG CCAGGCAGCA 
ATAAGGACTG ACTCCCTGGA CGTGCAGGGC CTGGGTAGCC GGGAAGACCT GTTGTCAGAG 
GTGAGTGGGC CCTCCTGCCC TCTGACCCGC TCCTCATCCT TCTGGGGCGG GTCGAGCATC 
CAGGTGCAGC AGCGCTCCGG CAGCCAGAGC AAAGTCTCCA AGCACATCCG CCTGCCAGCC 
CCTTGCCCAG GCCTGGAACC CAGCTGGGCC AAGGACCCTC AAGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCAGCAG TCAGGAAGAA 
CCCCTGTCCC CACGGGACTT GAAAAAATGC TACAGTGTAG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGGGT CCTGGCTAGA CGAACAGAGG AGACACTCCA TCGCTGTCAG CTGCCTGGAC 
AGCGGCTCCC AGCCCCGCCT ATGTCCAAGC CCCTCAAGCC TCGGGGGCCA ACCTCTTGGG 
GGCCCTGGGA GCCGGCCTAA GAAAAAACTC AGCCCACCCA GTATCTCTAT AGACCCCCCG 
GAGAGCCAGG GCCCTCGGCC CCCATGCAGT CCTGGCGTCT GCCTCAGGAG GAGGGCGCCG 
GCCAGTGACT CGAAGGATCC CTCGGCCTCC AGCCCCCTTG ACAGCACGGC TGCCTCACCC 
TCCCCAAAGA AAGATGCGCT GAGTCTCTCT GGTTTGTCTT CTGACCCAAC AGACCTGGAT 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532072.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532135.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X7, mRNA.

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CloneID	OMu45467
Clone ID Related Accession (Same CDS sequence)	XM_006532072.4 , XM_006532072.3
Accession Version	XM_006532072.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532072.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA AGCCCAGTGC 
AAACCCTACT ACTCTGACTA CTCGCGCTTC CGGCTCCTCG TCCACCACCT GTGTACCAGC 
CACTACCTGG ACCTCTTCAT CACTGGTGTC ATCGGGCTGA ATGTGGTCAC GATGGCCATG 
GAACATTACC AGCAGCCCCA GATCCTGGAC GAGGCTCTGA AGATCTGCAA CTACATCTTT 
ACCGTCATCT TTGTCTTGGA GTCAGTATTC AAACTTGTGG CCTTCGGCTT CCGCCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCTATTGTGC TTCTGTCCAT CATGGGCATC 
ACGCTGGAAG AGATTGAGGT CAATGCTTCA CTGCCCATCA ACCCCACCAT CATCCGTATC 
ATGAGGGTGC TCCGCATTGC TCGAGTTCTG AAGCTGTTGA AGATGGCTGT GGGCATGCGG 
GCACTGCTGG ACACGGTGAT GCAGGCCCTG CCCCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGCTATTAT TTTTCATCTT TGCAGCTCTG GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACAC ACCCTTGTGA GGGCTTGGGC CGGCATGCCA CCTTTAGGAA CTTTGGTATG 
GCCTTTCTGA CCCTCTTCCG AGTCTCCACT GGTGACAACT GGAATGGTAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACC TGCTACAACA CCGTCATCTC ACCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG 
ATGAAGCACC TGGAAGAGAG CAACAAAGAG GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG 
CTGGAGCTAG AGATGAAGAC ACTCAGCCCG CAGCCCCACT CCCCGCTGGG CAGCCCCTTC 
CTCTGGCCTG GGGTGGAAGG TGTCAATAGC CCTGACAGCC CTAAGCCTGG GGCTCCACAC 
ACCACGGCCC ACATTGGAGC AGCCTCTTCA GGCTTCTCCC TTGAGCACCC CACGGACAGA 
CAGCTGTTTG ACACCATCTC CCTGCTGATC CAGGGCTCCC TGGAGGGGGA GCTGAAGCTG 
ATGGACGAGC TGGCAGGCCC AGGGGGCCAG CCCTCTGCCT TCCCTTCCGC CCCCAGCCCG 
GGCGACTCCG ACCCACAGAT CCCTCTAGCT GAGATGGAGG CTCTGTCTCT GACGTCAGAG 
ATTGTGTCTG AACCGTCCTG CTCTCTAGCT CTGACGGATG ACTCTTTGCC TGATGACACT 
CACACACTCT TACTTAGTGC CCTGGAGAGC AATATGGTAC CTCACACTGA GGAGGGGCCA 
GTCCCCCTAG GACCAGACCT GCTGACTGTG AGGAAGTCTG GTGTCAGCCG GACACACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGCAAT GGGAGCACTG CCGAGAGATC CCTAGGACAC 
AGGGGCTGGG GGCTCCCCAA AGCCCAGTCA GGCTCCATCT TGTCTGTTCA CTCCCAACCA 
GCAGACACCA GCTGCATCCT ACAGCTTCCC AAAGATGCAC ACTATCTGCT CCAGCCTCAT 
GGGGCTCCCA CCTGGGGCGC CATCCCTAAA CTACCCCCAC CTGGCCGCTC CCCTCTGGCT 
CAGAGGCCTC TCAGGCGCCA GGCAGCAATA AGGACTGACT CCCTGGACGT GCAGGGCCTG 
GGTAGCCGGG AAGACCTGTT GTCAGAGGTG AGTGGGCCCT CCTGCCCTCT GACCCGCTCC 
TCATCCTTCT GGGGCGGGTC GAGCATCCAG GTGCAGCAGC GCTCCGGCAG CCAGAGCAAA 
GTCTCCAAGC ACATCCGCCT GCCAGCCCCT TGCCCAGGCC TGGAACCCAG CTGGGCCAAG 
GACCCTCAAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCAGCAGTCA GGAAGAACCC CTGTCCCCAC GGGACTTGAA AAAATGCTAC 
AGTGTAGAGG CCCAGAGCTG CCGGCGCAGG CCTGGGTCCT GGCTAGACGA ACAGAGGAGA 
CACTCCATCG CTGTCAGCTG CCTGGACAGC GGCTCCCAGC CCCGCCTATG TCCAAGCCCC 
TCAAGCCTCG GGGGCCAACC TCTTGGGGGC CCTGGGAGCC GGCCTAAGAA AAAACTCAGC 
CCACCCAGTA TCTCTATAGA CCCCCCGGAG AGCCAGGGCC CTCGGCCCCC ATGCAGTCCT 
GGCGTCTGCC TCAGGAGGAG GGCGCCGGCC AGTGACTCGA AGGATCCCTC GGCCTCCAGC 
CCCCTTGACA GCACGGCTGC CTCACCCTCC CCAAAGAAAG ATGCGCTGAG TCTCTCTGGT 
TTGTCTTCTG ACCCAACAGA CCTGGATCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532135.1
CDS	773..7885
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532072.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532072.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA AGCCCAGTGC 
AAACCCTACT ACTCTGACTA CTCGCGCTTC CGGCTCCTCG TCCACCACCT GTGTACCAGC 
CACTACCTGG ACCTCTTCAT CACTGGTGTC ATCGGGCTGA ATGTGGTCAC GATGGCCATG 
GAACATTACC AGCAGCCCCA GATCCTGGAC GAGGCTCTGA AGATCTGCAA CTACATCTTT 
ACCGTCATCT TTGTCTTGGA GTCAGTATTC AAACTTGTGG CCTTCGGCTT CCGCCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCTATTGTGC TTCTGTCCAT CATGGGCATC 
ACGCTGGAAG AGATTGAGGT CAATGCTTCA CTGCCCATCA ACCCCACCAT CATCCGTATC 
ATGAGGGTGC TCCGCATTGC TCGAGTTCTG AAGCTGTTGA AGATGGCTGT GGGCATGCGG 
GCACTGCTGG ACACGGTGAT GCAGGCCCTG CCCCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGCTATTAT TTTTCATCTT TGCAGCTCTG GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACAC ACCCTTGTGA GGGCTTGGGC CGGCATGCCA CCTTTAGGAA CTTTGGTATG 
GCCTTTCTGA CCCTCTTCCG AGTCTCCACT GGTGACAACT GGAATGGTAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACC TGCTACAACA CCGTCATCTC ACCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG 
ATGAAGCACC TGGAAGAGAG CAACAAAGAG GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG 
CTGGAGCTAG AGATGAAGAC ACTCAGCCCG CAGCCCCACT CCCCGCTGGG CAGCCCCTTC 
CTCTGGCCTG GGGTGGAAGG TGTCAATAGC CCTGACAGCC CTAAGCCTGG GGCTCCACAC 
ACCACGGCCC ACATTGGAGC AGCCTCTTCA GGCTTCTCCC TTGAGCACCC CACGGACAGA 
CAGCTGTTTG ACACCATCTC CCTGCTGATC CAGGGCTCCC TGGAGGGGGA GCTGAAGCTG 
ATGGACGAGC TGGCAGGCCC AGGGGGCCAG CCCTCTGCCT TCCCTTCCGC CCCCAGCCCG 
GGCGACTCCG ACCCACAGAT CCCTCTAGCT GAGATGGAGG CTCTGTCTCT GACGTCAGAG 
ATTGTGTCTG AACCGTCCTG CTCTCTAGCT CTGACGGATG ACTCTTTGCC TGATGACACT 
CACACACTCT TACTTAGTGC CCTGGAGAGC AATATGGTAC CTCACACTGA GGAGGGGCCA 
GTCCCCCTAG GACCAGACCT GCTGACTGTG AGGAAGTCTG GTGTCAGCCG GACACACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGCAAT GGGAGCACTG CCGAGAGATC CCTAGGACAC 
AGGGGCTGGG GGCTCCCCAA AGCCCAGTCA GGCTCCATCT TGTCTGTTCA CTCCCAACCA 
GCAGACACCA GCTGCATCCT ACAGCTTCCC AAAGATGCAC ACTATCTGCT CCAGCCTCAT 
GGGGCTCCCA CCTGGGGCGC CATCCCTAAA CTACCCCCAC CTGGCCGCTC CCCTCTGGCT 
CAGAGGCCTC TCAGGCGCCA GGCAGCAATA AGGACTGACT CCCTGGACGT GCAGGGCCTG 
GGTAGCCGGG AAGACCTGTT GTCAGAGGTG AGTGGGCCCT CCTGCCCTCT GACCCGCTCC 
TCATCCTTCT GGGGCGGGTC GAGCATCCAG GTGCAGCAGC GCTCCGGCAG CCAGAGCAAA 
GTCTCCAAGC ACATCCGCCT GCCAGCCCCT TGCCCAGGCC TGGAACCCAG CTGGGCCAAG 
GACCCTCAAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCAGCAGTCA GGAAGAACCC CTGTCCCCAC GGGACTTGAA AAAATGCTAC 
AGTGTAGAGG CCCAGAGCTG CCGGCGCAGG CCTGGGTCCT GGCTAGACGA ACAGAGGAGA 
CACTCCATCG CTGTCAGCTG CCTGGACAGC GGCTCCCAGC CCCGCCTATG TCCAAGCCCC 
TCAAGCCTCG GGGGCCAACC TCTTGGGGGC CCTGGGAGCC GGCCTAAGAA AAAACTCAGC 
CCACCCAGTA TCTCTATAGA CCCCCCGGAG AGCCAGGGCC CTCGGCCCCC ATGCAGTCCT 
GGCGTCTGCC TCAGGAGGAG GGCGCCGGCC AGTGACTCGA AGGATCCCTC GGCCTCCAGC 
CCCCTTGACA GCACGGCTGC CTCACCCTCC CCAAAGAAAG ATGCGCTGAG TCTCTCTGGT 
TTGTCTTCTG ACCCAACAGA CCTGGATCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532073.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532136.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X10, mRNA.

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CloneID	OMu45468
Clone ID Related Accession (Same CDS sequence)	XM_006532073.3 , XM_006532073.4
Accession Version	XM_006532073.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532073.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG ATCTAATGTT GGACGATGTA 
ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG TCAGAAGCCC AGTGCAAACC CTACTACTCT 
GACTACTCGC GCTTCCGGCT CCTCGTCCAC CACCTGTGTA CCAGCCACTA CCTGGACCTC 
TTCATCACTG GTGTCATCGG GCTGAATGTG GTCACGATGG CCATGGAACA TTACCAGCAG 
CCCCAGATCC TGGACGAGGC TCTGAAGATC TGCAACTACA TCTTTACCGT CATCTTTGTC 
TTGGAGTCAG TATTCAAACT TGTGGCCTTC GGCTTCCGCC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCTAT TGTGCTTCTG TCCATCATGG GCATCACGCT GGAAGAGATT 
GAGGTCAATG CTTCACTGCC CATCAACCCC ACCATCATCC GTATCATGAG GGTGCTCCGC 
ATTGCTCGAG TTCTGAAGCT GTTGAAGATG GCTGTGGGCA TGCGGGCACT GCTGGACACG 
GTGATGCAGG CCCTGCCCCA GGTGGGGAAC CTGGGACTTC TCTTCATGCT ATTATTTTTC 
ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACACACCCT 
TGTGAGGGCT TGGGCCGGCA TGCCACCTTT AGGAACTTTG GTATGGCCTT TCTGACCCTC 
TTCCGAGTCT CCACTGGTGA CAACTGGAAT GGTATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACCTGCTA CAACACCGTC ATCTCACCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTCATAGCCG TGCTGATGAA GCACCTGGAA 
GAGAGCAACA AAGAGGCCAA GGAGGAGGCG GAGTTGGAGG CGGAGCTGGA GCTAGAGATG 
AAGACACTCA GCCCGCAGCC CCACTCCCCG CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG 
GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG CCTGGGGCTC CACACACCAC GGCCCACATT 
GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG CACCCCACGG ACAGACAGCT GTTTGACACC 
ATCTCCCTGC TGATCCAGGG CTCCCTGGAG GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT TCCGCCCCCA GCCCGGGCGA CTCCGACCCA 
CAGATCCCTC TAGCTGAGAT GGAGGCTCTG TCTCTGACGT CAGAGATTGT GTCTGAACCG 
TCCTGCTCTC TAGCTCTGAC GGATGACTCT TTGCCTGATG ACACTCACAC ACTCTTACTT 
AGTGCCCTGG AGAGCAATAT GGTACCTCAC ACTGAGGAGG GGCCAGTCCC CCTAGGACCA 
GACCTGCTGA CTGTGAGGAA GTCTGGTGTC AGCCGGACAC ACTCTCTGCC CAATGACAGC 
TACATGTGCC GCAATGGGAG CACTGCCGAG AGATCCCTAG GACACAGGGG CTGGGGGCTC 
CCCAAAGCCC AGTCAGGCTC CATCTTGTCT GTTCACTCCC AACCAGCAGA CACCAGCTGC 
ATCCTACAGC TTCCCAAAGA TGCACACTAT CTGCTCCAGC CTCATGGGGC TCCCACCTGG 
GGCGCCATCC CTAAACTACC CCCACCTGGC CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG 
CGCCAGGCAG CAATAAGGAC TGACTCCCTG GACGTGCAGG GCCTGGGTAG CCGGGAAGAC 
CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC CCTCTGACCC GCTCCTCATC CTTCTGGGGC 
GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC GGCAGCCAGA GCAAAGTCTC CAAGCACATC 
CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA CCCAGCTGGG CCAAGGACCC TCAAGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGCCCAGC 
AGTCAGGAAG AACCCCTGTC CCCACGGGAC TTGAAAAAAT GCTACAGTGT AGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA GACGAACAGA GGAGACACTC CATCGCTGTC 
AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC CTATGTCCAA GCCCCTCAAG CCTCGGGGGC 
CAACCTCTTG GGGGCCCTGG GAGCCGGCCT AAGAAAAAAC TCAGCCCACC CAGTATCTCT 
ATAGACCCCC CGGAGAGCCA GGGCCCTCGG CCCCCATGCA GTCCTGGCGT CTGCCTCAGG 
AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT CCCTCGGCCT CCAGCCCCCT TGACAGCACG 
GCTGCCTCAC CCTCCCCAAA GAAAGATGCG CTGAGTCTCT CTGGTTTGTC TTCTGACCCA 
ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532136.1
CDS	773..7870
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532073.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532073.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG ATCTAATGTT GGACGATGTA 
ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG TCAGAAGCCC AGTGCAAACC CTACTACTCT 
GACTACTCGC GCTTCCGGCT CCTCGTCCAC CACCTGTGTA CCAGCCACTA CCTGGACCTC 
TTCATCACTG GTGTCATCGG GCTGAATGTG GTCACGATGG CCATGGAACA TTACCAGCAG 
CCCCAGATCC TGGACGAGGC TCTGAAGATC TGCAACTACA TCTTTACCGT CATCTTTGTC 
TTGGAGTCAG TATTCAAACT TGTGGCCTTC GGCTTCCGCC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCTAT TGTGCTTCTG TCCATCATGG GCATCACGCT GGAAGAGATT 
GAGGTCAATG CTTCACTGCC CATCAACCCC ACCATCATCC GTATCATGAG GGTGCTCCGC 
ATTGCTCGAG TTCTGAAGCT GTTGAAGATG GCTGTGGGCA TGCGGGCACT GCTGGACACG 
GTGATGCAGG CCCTGCCCCA GGTGGGGAAC CTGGGACTTC TCTTCATGCT ATTATTTTTC 
ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACACACCCT 
TGTGAGGGCT TGGGCCGGCA TGCCACCTTT AGGAACTTTG GTATGGCCTT TCTGACCCTC 
TTCCGAGTCT CCACTGGTGA CAACTGGAAT GGTATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACCTGCTA CAACACCGTC ATCTCACCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTCATAGCCG TGCTGATGAA GCACCTGGAA 
GAGAGCAACA AAGAGGCCAA GGAGGAGGCG GAGTTGGAGG CGGAGCTGGA GCTAGAGATG 
AAGACACTCA GCCCGCAGCC CCACTCCCCG CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG 
GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG CCTGGGGCTC CACACACCAC GGCCCACATT 
GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG CACCCCACGG ACAGACAGCT GTTTGACACC 
ATCTCCCTGC TGATCCAGGG CTCCCTGGAG GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT TCCGCCCCCA GCCCGGGCGA CTCCGACCCA 
CAGATCCCTC TAGCTGAGAT GGAGGCTCTG TCTCTGACGT CAGAGATTGT GTCTGAACCG 
TCCTGCTCTC TAGCTCTGAC GGATGACTCT TTGCCTGATG ACACTCACAC ACTCTTACTT 
AGTGCCCTGG AGAGCAATAT GGTACCTCAC ACTGAGGAGG GGCCAGTCCC CCTAGGACCA 
GACCTGCTGA CTGTGAGGAA GTCTGGTGTC AGCCGGACAC ACTCTCTGCC CAATGACAGC 
TACATGTGCC GCAATGGGAG CACTGCCGAG AGATCCCTAG GACACAGGGG CTGGGGGCTC 
CCCAAAGCCC AGTCAGGCTC CATCTTGTCT GTTCACTCCC AACCAGCAGA CACCAGCTGC 
ATCCTACAGC TTCCCAAAGA TGCACACTAT CTGCTCCAGC CTCATGGGGC TCCCACCTGG 
GGCGCCATCC CTAAACTACC CCCACCTGGC CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG 
CGCCAGGCAG CAATAAGGAC TGACTCCCTG GACGTGCAGG GCCTGGGTAG CCGGGAAGAC 
CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC CCTCTGACCC GCTCCTCATC CTTCTGGGGC 
GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC GGCAGCCAGA GCAAAGTCTC CAAGCACATC 
CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA CCCAGCTGGG CCAAGGACCC TCAAGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGCCCAGC 
AGTCAGGAAG AACCCCTGTC CCCACGGGAC TTGAAAAAAT GCTACAGTGT AGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA GACGAACAGA GGAGACACTC CATCGCTGTC 
AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC CTATGTCCAA GCCCCTCAAG CCTCGGGGGC 
CAACCTCTTG GGGGCCCTGG GAGCCGGCCT AAGAAAAAAC TCAGCCCACC CAGTATCTCT 
ATAGACCCCC CGGAGAGCCA GGGCCCTCGG CCCCCATGCA GTCCTGGCGT CTGCCTCAGG 
AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT CCCTCGGCCT CCAGCCCCCT TGACAGCACG 
GCTGCCTCAC CCTCCCCAAA GAAAGATGCG CTGAGTCTCT CTGGTTTGTC TTCTGACCCA 
ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532074.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532137.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X11, mRNA.

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CloneID	OMu45469
Clone ID Related Accession (Same CDS sequence)	XM_006532074.3 , XM_006532074.4
Accession Version	XM_006532074.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAA GCCCAGTGCA AACCCTACTA CTCTGACTAC 
TCGCGCTTCC GGCTCCTCGT CCACCACCTG TGTACCAGCC ACTACCTGGA CCTCTTCATC 
ACTGGTGTCA TCGGGCTGAA TGTGGTCACG ATGGCCATGG AACATTACCA GCAGCCCCAG 
ATCCTGGACG AGGCTCTGAA GATCTGCAAC TACATCTTTA CCGTCATCTT TGTCTTGGAG 
TCAGTATTCA AACTTGTGGC CTTCGGCTTC CGCCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CTATTGTGCT TCTGTCCATC ATGGGCATCA CGCTGGAAGA GATTGAGGTC 
AATGCTTCAC TGCCCATCAA CCCCACCATC ATCCGTATCA TGAGGGTGCT CCGCATTGCT 
CGAGTTCTGA AGCTGTTGAA GATGGCTGTG GGCATGCGGG CACTGCTGGA CACGGTGATG 
CAGGCCCTGC CCCAGGTGGG GAACCTGGGA CTTCTCTTCA TGCTATTATT TTTCATCTTT 
GCAGCTCTGG GCGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACACA CCCTTGTGAG 
GGCTTGGGCC GGCATGCCAC CTTTAGGAAC TTTGGTATGG CCTTTCTGAC CCTCTTCCGA 
GTCTCCACTG GTGACAACTG GAATGGTATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACCT GCTACAACAC CGTCATCTCA CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTCATA GCCGTGCTGA TGAAGCACCT GGAAGAGAGC 
AACAAAGAGG CCAAGGAGGA GGCGGAGTTG GAGGCGGAGC TGGAGCTAGA GATGAAGACA 
CTCAGCCCGC AGCCCCACTC CCCGCTGGGC AGCCCCTTCC TCTGGCCTGG GGTGGAAGGT 
GTCAATAGCC CTGACAGCCC TAAGCCTGGG GCTCCACACA CCACGGCCCA CATTGGAGCA 
GCCTCTTCAG GCTTCTCCCT TGAGCACCCC ACGGACAGAC AGCTGTTTGA CACCATCTCC 
CTGCTGATCC AGGGCTCCCT GGAGGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA 
GGGGGCCAGC CCTCTGCCTT CCCTTCCGCC CCCAGCCCGG GCGACTCCGA CCCACAGATC 
CCTCTAGCTG AGATGGAGGC TCTGTCTCTG ACGTCAGAGA TTGTGTCTGA ACCGTCCTGC 
TCTCTAGCTC TGACGGATGA CTCTTTGCCT GATGACACTC ACACACTCTT ACTTAGTGCC 
CTGGAGAGCA ATATGGTACC TCACACTGAG GAGGGGCCAG TCCCCCTAGG ACCAGACCTG 
CTGACTGTGA GGAAGTCTGG TGTCAGCCGG ACACACTCTC TGCCCAATGA CAGCTACATG 
TGCCGCAATG GGAGCACTGC CGAGAGATCC CTAGGACACA GGGGCTGGGG GCTCCCCAAA 
GCCCAGTCAG GCTCCATCTT GTCTGTTCAC TCCCAACCAG CAGACACCAG CTGCATCCTA 
CAGCTTCCCA AAGATGCACA CTATCTGCTC CAGCCTCATG GGGCTCCCAC CTGGGGCGCC 
ATCCCTAAAC TACCCCCACC TGGCCGCTCC CCTCTGGCTC AGAGGCCTCT CAGGCGCCAG 
GCAGCAATAA GGACTGACTC CCTGGACGTG CAGGGCCTGG GTAGCCGGGA AGACCTGTTG 
TCAGAGGTGA GTGGGCCCTC CTGCCCTCTG ACCCGCTCCT CATCCTTCTG GGGCGGGTCG 
AGCATCCAGG TGCAGCAGCG CTCCGGCAGC CAGAGCAAAG TCTCCAAGCA CATCCGCCTG 
CCAGCCCCTT GCCCAGGCCT GGAACCCAGC TGGGCCAAGG ACCCTCAAGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CAGCAGTCAG 
GAAGAACCCC TGTCCCCACG GGACTTGAAA AAATGCTACA GTGTAGAGGC CCAGAGCTGC 
CGGCGCAGGC CTGGGTCCTG GCTAGACGAA CAGAGGAGAC ACTCCATCGC TGTCAGCTGC 
CTGGACAGCG GCTCCCAGCC CCGCCTATGT CCAAGCCCCT CAAGCCTCGG GGGCCAACCT 
CTTGGGGGCC CTGGGAGCCG GCCTAAGAAA AAACTCAGCC CACCCAGTAT CTCTATAGAC 
CCCCCGGAGA GCCAGGGCCC TCGGCCCCCA TGCAGTCCTG GCGTCTGCCT CAGGAGGAGG 
GCGCCGGCCA GTGACTCGAA GGATCCCTCG GCCTCCAGCC CCCTTGACAG CACGGCTGCC 
TCACCCTCCC CAAAGAAAGA TGCGCTGAGT CTCTCTGGTT TGTCTTCTGA CCCAACAGAC 
CTGGATCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532137.1
CDS	773..7864
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532074.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532074.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAA GCCCAGTGCA AACCCTACTA CTCTGACTAC 
TCGCGCTTCC GGCTCCTCGT CCACCACCTG TGTACCAGCC ACTACCTGGA CCTCTTCATC 
ACTGGTGTCA TCGGGCTGAA TGTGGTCACG ATGGCCATGG AACATTACCA GCAGCCCCAG 
ATCCTGGACG AGGCTCTGAA GATCTGCAAC TACATCTTTA CCGTCATCTT TGTCTTGGAG 
TCAGTATTCA AACTTGTGGC CTTCGGCTTC CGCCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CTATTGTGCT TCTGTCCATC ATGGGCATCA CGCTGGAAGA GATTGAGGTC 
AATGCTTCAC TGCCCATCAA CCCCACCATC ATCCGTATCA TGAGGGTGCT CCGCATTGCT 
CGAGTTCTGA AGCTGTTGAA GATGGCTGTG GGCATGCGGG CACTGCTGGA CACGGTGATG 
CAGGCCCTGC CCCAGGTGGG GAACCTGGGA CTTCTCTTCA TGCTATTATT TTTCATCTTT 
GCAGCTCTGG GCGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACACA CCCTTGTGAG 
GGCTTGGGCC GGCATGCCAC CTTTAGGAAC TTTGGTATGG CCTTTCTGAC CCTCTTCCGA 
GTCTCCACTG GTGACAACTG GAATGGTATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACCT GCTACAACAC CGTCATCTCA CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTCATA GCCGTGCTGA TGAAGCACCT GGAAGAGAGC 
AACAAAGAGG CCAAGGAGGA GGCGGAGTTG GAGGCGGAGC TGGAGCTAGA GATGAAGACA 
CTCAGCCCGC AGCCCCACTC CCCGCTGGGC AGCCCCTTCC TCTGGCCTGG GGTGGAAGGT 
GTCAATAGCC CTGACAGCCC TAAGCCTGGG GCTCCACACA CCACGGCCCA CATTGGAGCA 
GCCTCTTCAG GCTTCTCCCT TGAGCACCCC ACGGACAGAC AGCTGTTTGA CACCATCTCC 
CTGCTGATCC AGGGCTCCCT GGAGGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA 
GGGGGCCAGC CCTCTGCCTT CCCTTCCGCC CCCAGCCCGG GCGACTCCGA CCCACAGATC 
CCTCTAGCTG AGATGGAGGC TCTGTCTCTG ACGTCAGAGA TTGTGTCTGA ACCGTCCTGC 
TCTCTAGCTC TGACGGATGA CTCTTTGCCT GATGACACTC ACACACTCTT ACTTAGTGCC 
CTGGAGAGCA ATATGGTACC TCACACTGAG GAGGGGCCAG TCCCCCTAGG ACCAGACCTG 
CTGACTGTGA GGAAGTCTGG TGTCAGCCGG ACACACTCTC TGCCCAATGA CAGCTACATG 
TGCCGCAATG GGAGCACTGC CGAGAGATCC CTAGGACACA GGGGCTGGGG GCTCCCCAAA 
GCCCAGTCAG GCTCCATCTT GTCTGTTCAC TCCCAACCAG CAGACACCAG CTGCATCCTA 
CAGCTTCCCA AAGATGCACA CTATCTGCTC CAGCCTCATG GGGCTCCCAC CTGGGGCGCC 
ATCCCTAAAC TACCCCCACC TGGCCGCTCC CCTCTGGCTC AGAGGCCTCT CAGGCGCCAG 
GCAGCAATAA GGACTGACTC CCTGGACGTG CAGGGCCTGG GTAGCCGGGA AGACCTGTTG 
TCAGAGGTGA GTGGGCCCTC CTGCCCTCTG ACCCGCTCCT CATCCTTCTG GGGCGGGTCG 
AGCATCCAGG TGCAGCAGCG CTCCGGCAGC CAGAGCAAAG TCTCCAAGCA CATCCGCCTG 
CCAGCCCCTT GCCCAGGCCT GGAACCCAGC TGGGCCAAGG ACCCTCAAGA GACCAGAAGC 
AGCTTAGAGC TGGACACGGA GCTGAGCTGG ATTTCAGGAG ACCTCCTGCC CAGCAGTCAG 
GAAGAACCCC TGTCCCCACG GGACTTGAAA AAATGCTACA GTGTAGAGGC CCAGAGCTGC 
CGGCGCAGGC CTGGGTCCTG GCTAGACGAA CAGAGGAGAC ACTCCATCGC TGTCAGCTGC 
CTGGACAGCG GCTCCCAGCC CCGCCTATGT CCAAGCCCCT CAAGCCTCGG GGGCCAACCT 
CTTGGGGGCC CTGGGAGCCG GCCTAAGAAA AAACTCAGCC CACCCAGTAT CTCTATAGAC 
CCCCCGGAGA GCCAGGGCCC TCGGCCCCCA TGCAGTCCTG GCGTCTGCCT CAGGAGGAGG 
GCGCCGGCCA GTGACTCGAA GGATCCCTCG GCCTCCAGCC CCCTTGACAG CACGGCTGCC 
TCACCCTCCC CAAAGAAAGA TGCGCTGAGT CTCTCTGGTT TGTCTTCTGA CCCAACAGAC 
CTGGATCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532075.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532138.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X12, mRNA.

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CloneID	OMu45470
Clone ID Related Accession (Same CDS sequence)	XM_006532075.3 , XM_006532075.4
Accession Version	XM_006532075.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532075.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAA TCTAATGTTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCTGCGT CAGAAGCCCA GTGCAAACCC TACTACTCTG ACTACTCGCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGACCTCT TCATCACTGG TGTCATCGGG 
CTGAATGTGG TCACGATGGC CATGGAACAT TACCAGCAGC CCCAGATCCT GGACGAGGCT 
CTGAAGATCT GCAACTACAT CTTTACCGTC ATCTTTGTCT TGGAGTCAGT ATTCAAACTT 
GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCTATT 
GTGCTTCTGT CCATCATGGG CATCACGCTG GAAGAGATTG AGGTCAATGC TTCACTGCCC 
ATCAACCCCA CCATCATCCG TATCATGAGG GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG 
TTGAAGATGG CTGTGGGCAT GCGGGCACTG CTGGACACGG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACC TGGGACTTCT CTTCATGCTA TTATTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGACCTGGA GTGTGATGAG ACACACCCTT GTGAGGGCTT GGGCCGGCAT 
GCCACCTTTA GGAACTTTGG TATGGCCTTT CTGACCCTCT TCCGAGTCTC CACTGGTGAC 
AACTGGAATG GTATTATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACCGTCA TCTCACCCAT CTACTTCGTG TCCTTCGTGC TGACGGCCCA GTTTGTGCTG 
GTCAACGTGG TCATAGCCGT GCTGATGAAG CACCTGGAAG AGAGCAACAA AGAGGCCAAG 
GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG CTAGAGATGA AGACACTCAG CCCGCAGCCC 
CACTCCCCGC TGGGCAGCCC CTTCCTCTGG CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC 
AGCCCTAAGC CTGGGGCTCC ACACACCACG GCCCACATTG GAGCAGCCTC TTCAGGCTTC 
TCCCTTGAGC ACCCCACGGA CAGACAGCTG TTTGACACCA TCTCCCTGCT GATCCAGGGC 
TCCCTGGAGG GGGAGCTGAA GCTGATGGAC GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT 
GCCTTCCCTT CCGCCCCCAG CCCGGGCGAC TCCGACCCAC AGATCCCTCT AGCTGAGATG 
GAGGCTCTGT CTCTGACGTC AGAGATTGTG TCTGAACCGT CCTGCTCTCT AGCTCTGACG 
GATGACTCTT TGCCTGATGA CACTCACACA CTCTTACTTA GTGCCCTGGA GAGCAATATG 
GTACCTCACA CTGAGGAGGG GCCAGTCCCC CTAGGACCAG ACCTGCTGAC TGTGAGGAAG 
TCTGGTGTCA GCCGGACACA CTCTCTGCCC AATGACAGCT ACATGTGCCG CAATGGGAGC 
ACTGCCGAGA GATCCCTAGG ACACAGGGGC TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC 
ATCTTGTCTG TTCACTCCCA ACCAGCAGAC ACCAGCTGCA TCCTACAGCT TCCCAAAGAT 
GCACACTATC TGCTCCAGCC TCATGGGGCT CCCACCTGGG GCGCCATCCC TAAACTACCC 
CCACCTGGCC GCTCCCCTCT GGCTCAGAGG CCTCTCAGGC GCCAGGCAGC AATAAGGACT 
GACTCCCTGG ACGTGCAGGG CCTGGGTAGC CGGGAAGACC TGTTGTCAGA GGTGAGTGGG 
CCCTCCTGCC CTCTGACCCG CTCCTCATCC TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG 
CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC AAGCACATCC GCCTGCCAGC CCCTTGCCCA 
GGCCTGGAAC CCAGCTGGGC CAAGGACCCT CAAGAGACCA GAAGCAGCTT AGAGCTGGAC 
ACGGAGCTGA GCTGGATTTC AGGAGACCTC CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC 
CCACGGGACT TGAAAAAATG CTACAGTGTA GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG 
TCCTGGCTAG ACGAACAGAG GAGACACTCC ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC 
CAGCCCCGCC TATGTCCAAG CCCCTCAAGC CTCGGGGGCC AACCTCTTGG GGGCCCTGGG 
AGCCGGCCTA AGAAAAAACT CAGCCCACCC AGTATCTCTA TAGACCCCCC GGAGAGCCAG 
GGCCCTCGGC CCCCATGCAG TCCTGGCGTC TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC 
TCGAAGGATC CCTCGGCCTC CAGCCCCCTT GACAGCACGG CTGCCTCACC CTCCCCAAAG 
AAAGATGCGC TGAGTCTCTC TGGTTTGTCT TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532138.1
CDS	773..7849
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532075.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532075.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAA TCTAATGTTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCTGCGT CAGAAGCCCA GTGCAAACCC TACTACTCTG ACTACTCGCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGACCTCT TCATCACTGG TGTCATCGGG 
CTGAATGTGG TCACGATGGC CATGGAACAT TACCAGCAGC CCCAGATCCT GGACGAGGCT 
CTGAAGATCT GCAACTACAT CTTTACCGTC ATCTTTGTCT TGGAGTCAGT ATTCAAACTT 
GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCTATT 
GTGCTTCTGT CCATCATGGG CATCACGCTG GAAGAGATTG AGGTCAATGC TTCACTGCCC 
ATCAACCCCA CCATCATCCG TATCATGAGG GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG 
TTGAAGATGG CTGTGGGCAT GCGGGCACTG CTGGACACGG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACC TGGGACTTCT CTTCATGCTA TTATTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGACCTGGA GTGTGATGAG ACACACCCTT GTGAGGGCTT GGGCCGGCAT 
GCCACCTTTA GGAACTTTGG TATGGCCTTT CTGACCCTCT TCCGAGTCTC CACTGGTGAC 
AACTGGAATG GTATTATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACCGTCA TCTCACCCAT CTACTTCGTG TCCTTCGTGC TGACGGCCCA GTTTGTGCTG 
GTCAACGTGG TCATAGCCGT GCTGATGAAG CACCTGGAAG AGAGCAACAA AGAGGCCAAG 
GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG CTAGAGATGA AGACACTCAG CCCGCAGCCC 
CACTCCCCGC TGGGCAGCCC CTTCCTCTGG CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC 
AGCCCTAAGC CTGGGGCTCC ACACACCACG GCCCACATTG GAGCAGCCTC TTCAGGCTTC 
TCCCTTGAGC ACCCCACGGA CAGACAGCTG TTTGACACCA TCTCCCTGCT GATCCAGGGC 
TCCCTGGAGG GGGAGCTGAA GCTGATGGAC GAGCTGGCAG GCCCAGGGGG CCAGCCCTCT 
GCCTTCCCTT CCGCCCCCAG CCCGGGCGAC TCCGACCCAC AGATCCCTCT AGCTGAGATG 
GAGGCTCTGT CTCTGACGTC AGAGATTGTG TCTGAACCGT CCTGCTCTCT AGCTCTGACG 
GATGACTCTT TGCCTGATGA CACTCACACA CTCTTACTTA GTGCCCTGGA GAGCAATATG 
GTACCTCACA CTGAGGAGGG GCCAGTCCCC CTAGGACCAG ACCTGCTGAC TGTGAGGAAG 
TCTGGTGTCA GCCGGACACA CTCTCTGCCC AATGACAGCT ACATGTGCCG CAATGGGAGC 
ACTGCCGAGA GATCCCTAGG ACACAGGGGC TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC 
ATCTTGTCTG TTCACTCCCA ACCAGCAGAC ACCAGCTGCA TCCTACAGCT TCCCAAAGAT 
GCACACTATC TGCTCCAGCC TCATGGGGCT CCCACCTGGG GCGCCATCCC TAAACTACCC 
CCACCTGGCC GCTCCCCTCT GGCTCAGAGG CCTCTCAGGC GCCAGGCAGC AATAAGGACT 
GACTCCCTGG ACGTGCAGGG CCTGGGTAGC CGGGAAGACC TGTTGTCAGA GGTGAGTGGG 
CCCTCCTGCC CTCTGACCCG CTCCTCATCC TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG 
CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC AAGCACATCC GCCTGCCAGC CCCTTGCCCA 
GGCCTGGAAC CCAGCTGGGC CAAGGACCCT CAAGAGACCA GAAGCAGCTT AGAGCTGGAC 
ACGGAGCTGA GCTGGATTTC AGGAGACCTC CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC 
CCACGGGACT TGAAAAAATG CTACAGTGTA GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG 
TCCTGGCTAG ACGAACAGAG GAGACACTCC ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC 
CAGCCCCGCC TATGTCCAAG CCCCTCAAGC CTCGGGGGCC AACCTCTTGG GGGCCCTGGG 
AGCCGGCCTA AGAAAAAACT CAGCCCACCC AGTATCTCTA TAGACCCCCC GGAGAGCCAG 
GGCCCTCGGC CCCCATGCAG TCCTGGCGTC TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC 
TCGAAGGATC CCTCGGCCTC CAGCCCCCTT GACAGCACGG CTGCCTCACC CTCCCCAAAG 
AAAGATGCGC TGAGTCTCTC TGGTTTGTCT TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532076.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532139.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X13, mRNA.

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CloneID	OMu45471
Clone ID Related Accession (Same CDS sequence)	XM_006532076.3 , XM_006532076.4
Accession Version	XM_006532076.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGGACAG ACAGCTGTTT 
GACACCATCT CCCTGCTGAT CCAGGGCTCC CTGGAGGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTTCCG CCCCCAGCCC GGGCGACTCC 
GACCCACAGA TCCCTCTAGC TGAGATGGAG GCTCTGTCTC TGACGTCAGA GATTGTGTCT 
GAACCGTCCT GCTCTCTAGC TCTGACGGAT GACTCTTTGC CTGATGACAC TCACACACTC 
TTACTTAGTG CCCTGGAGAG CAATATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532139.1
CDS	773..7816
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532076.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532076.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGGACAG ACAGCTGTTT 
GACACCATCT CCCTGCTGAT CCAGGGCTCC CTGGAGGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTTCCG CCCCCAGCCC GGGCGACTCC 
GACCCACAGA TCCCTCTAGC TGAGATGGAG GCTCTGTCTC TGACGTCAGA GATTGTGTCT 
GAACCGTCCT GCTCTCTAGC TCTGACGGAT GACTCTTTGC CTGATGACAC TCACACACTC 
TTACTTAGTG CCCTGGAGAG CAATATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532079.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532142.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X16, mRNA.

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CloneID	OMu45474
Clone ID Related Accession (Same CDS sequence)	XM_006532079.3 , XM_006532079.4
Accession Version	XM_006532079.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532079.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAT CTAATGTTGG ACGATGTAAT TGCTTCCGGC 
AGCTCAGCCA GCGCTGCGTC AGAAGCCCAG TGCAAACCCT ACTACTCTGA CTACTCGCGC 
TTCCGGCTCC TCGTCCACCA CCTGTGTACC AGCCACTACC TGGACCTCTT CATCACTGGT 
GTCATCGGGC TGAATGTGGT CACGATGGCC ATGGAACATT ACCAGCAGCC CCAGATCCTG 
GACGAGGCTC TGAAGATCTG CAACTACATC TTTACCGTCA TCTTTGTCTT GGAGTCAGTA 
TTCAAACTTG TGGCCTTCGG CTTCCGCCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCTATTG TGCTTCTGTC CATCATGGGC ATCACGCTGG AAGAGATTGA GGTCAATGCT 
TCACTGCCCA TCAACCCCAC CATCATCCGT ATCATGAGGG TGCTCCGCAT TGCTCGAGTT 
CTGAAGCTGT TGAAGATGGC TGTGGGCATG CGGGCACTGC TGGACACGGT GATGCAGGCC 
CTGCCCCAGG TGGGGAACCT GGGACTTCTC TTCATGCTAT TATTTTTCAT CTTTGCAGCT 
CTGGGCGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CACACCCTTG TGAGGGCTTG 
GGCCGGCATG CCACCTTTAG GAACTTTGGT ATGGCCTTTC TGACCCTCTT CCGAGTCTCC 
ACTGGTGACA ACTGGAATGG TATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACCTGCTACA ACACCGTCAT CTCACCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT CATAGCCGTG CTGATGAAGC ACCTGGAAGA GAGCAACAAA 
GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG GAGCTGGAGC TAGAGATGAA GACACTCAGC 
CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT 
AGCCCTGACA GCCCTAAGCC TGGGGCTCCA CACACCACGG CCCACATTGG AGCAGCCTCT 
TCAGGCTTCT CCCTTGAGCA CCCCACGGAC AGACAGCTGT TTGACACCAT CTCCCTGCTG 
ATCCAGGGCT CCCTGGAGGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTTC CGCCCCCAGC CCGGGCGACT CCGACCCACA GATGGTACCT 
CACACTGAGG AGGGGCCAGT CCCCCTAGGA CCAGACCTGC TGACTGTGAG GAAGTCTGGT 
GTCAGCCGGA CACACTCTCT GCCCAATGAC AGCTACATGT GCCGCAATGG GAGCACTGCC 
GAGAGATCCC TAGGACACAG GGGCTGGGGG CTCCCCAAAG CCCAGTCAGG CTCCATCTTG 
TCTGTTCACT CCCAACCAGC AGACACCAGC TGCATCCTAC AGCTTCCCAA AGATGCACAC 
TATCTGCTCC AGCCTCATGG GGCTCCCACC TGGGGCGCCA TCCCTAAACT ACCCCCACCT 
GGCCGCTCCC CTCTGGCTCA GAGGCCTCTC AGGCGCCAGG CAGCAATAAG GACTGACTCC 
CTGGACGTGC AGGGCCTGGG TAGCCGGGAA GACCTGTTGT CAGAGGTGAG TGGGCCCTCC 
TGCCCTCTGA CCCGCTCCTC ATCCTTCTGG GGCGGGTCGA GCATCCAGGT GCAGCAGCGC 
TCCGGCAGCC AGAGCAAAGT CTCCAAGCAC ATCCGCCTGC CAGCCCCTTG CCCAGGCCTG 
GAACCCAGCT GGGCCAAGGA CCCTCAAGAG ACCAGAAGCA GCTTAGAGCT GGACACGGAG 
CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC AGCAGTCAGG AAGAACCCCT GTCCCCACGG 
GACTTGAAAA AATGCTACAG TGTAGAGGCC CAGAGCTGCC GGCGCAGGCC TGGGTCCTGG 
CTAGACGAAC AGAGGAGACA CTCCATCGCT GTCAGCTGCC TGGACAGCGG CTCCCAGCCC 
CGCCTATGTC CAAGCCCCTC AAGCCTCGGG GGCCAACCTC TTGGGGGCCC TGGGAGCCGG 
CCTAAGAAAA AACTCAGCCC ACCCAGTATC TCTATAGACC CCCCGGAGAG CCAGGGCCCT 
CGGCCCCCAT GCAGTCCTGG CGTCTGCCTC AGGAGGAGGG CGCCGGCCAG TGACTCGAAG 
GATCCCTCGG CCTCCAGCCC CCTTGACAGC ACGGCTGCCT CACCCTCCCC AAAGAAAGAT 
GCGCTGAGTC TCTCTGGTTT GTCTTCTGAC CCAACAGACC TGGATCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532142.1
CDS	773..7783
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532079.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532079.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAT CTAATGTTGG ACGATGTAAT TGCTTCCGGC 
AGCTCAGCCA GCGCTGCGTC AGAAGCCCAG TGCAAACCCT ACTACTCTGA CTACTCGCGC 
TTCCGGCTCC TCGTCCACCA CCTGTGTACC AGCCACTACC TGGACCTCTT CATCACTGGT 
GTCATCGGGC TGAATGTGGT CACGATGGCC ATGGAACATT ACCAGCAGCC CCAGATCCTG 
GACGAGGCTC TGAAGATCTG CAACTACATC TTTACCGTCA TCTTTGTCTT GGAGTCAGTA 
TTCAAACTTG TGGCCTTCGG CTTCCGCCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCTATTG TGCTTCTGTC CATCATGGGC ATCACGCTGG AAGAGATTGA GGTCAATGCT 
TCACTGCCCA TCAACCCCAC CATCATCCGT ATCATGAGGG TGCTCCGCAT TGCTCGAGTT 
CTGAAGCTGT TGAAGATGGC TGTGGGCATG CGGGCACTGC TGGACACGGT GATGCAGGCC 
CTGCCCCAGG TGGGGAACCT GGGACTTCTC TTCATGCTAT TATTTTTCAT CTTTGCAGCT 
CTGGGCGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CACACCCTTG TGAGGGCTTG 
GGCCGGCATG CCACCTTTAG GAACTTTGGT ATGGCCTTTC TGACCCTCTT CCGAGTCTCC 
ACTGGTGACA ACTGGAATGG TATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACCTGCTACA ACACCGTCAT CTCACCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT CATAGCCGTG CTGATGAAGC ACCTGGAAGA GAGCAACAAA 
GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG GAGCTGGAGC TAGAGATGAA GACACTCAGC 
CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT 
AGCCCTGACA GCCCTAAGCC TGGGGCTCCA CACACCACGG CCCACATTGG AGCAGCCTCT 
TCAGGCTTCT CCCTTGAGCA CCCCACGGAC AGACAGCTGT TTGACACCAT CTCCCTGCTG 
ATCCAGGGCT CCCTGGAGGG GGAGCTGAAG CTGATGGACG AGCTGGCAGG CCCAGGGGGC 
CAGCCCTCTG CCTTCCCTTC CGCCCCCAGC CCGGGCGACT CCGACCCACA GATGGTACCT 
CACACTGAGG AGGGGCCAGT CCCCCTAGGA CCAGACCTGC TGACTGTGAG GAAGTCTGGT 
GTCAGCCGGA CACACTCTCT GCCCAATGAC AGCTACATGT GCCGCAATGG GAGCACTGCC 
GAGAGATCCC TAGGACACAG GGGCTGGGGG CTCCCCAAAG CCCAGTCAGG CTCCATCTTG 
TCTGTTCACT CCCAACCAGC AGACACCAGC TGCATCCTAC AGCTTCCCAA AGATGCACAC 
TATCTGCTCC AGCCTCATGG GGCTCCCACC TGGGGCGCCA TCCCTAAACT ACCCCCACCT 
GGCCGCTCCC CTCTGGCTCA GAGGCCTCTC AGGCGCCAGG CAGCAATAAG GACTGACTCC 
CTGGACGTGC AGGGCCTGGG TAGCCGGGAA GACCTGTTGT CAGAGGTGAG TGGGCCCTCC 
TGCCCTCTGA CCCGCTCCTC ATCCTTCTGG GGCGGGTCGA GCATCCAGGT GCAGCAGCGC 
TCCGGCAGCC AGAGCAAAGT CTCCAAGCAC ATCCGCCTGC CAGCCCCTTG CCCAGGCCTG 
GAACCCAGCT GGGCCAAGGA CCCTCAAGAG ACCAGAAGCA GCTTAGAGCT GGACACGGAG 
CTGAGCTGGA TTTCAGGAGA CCTCCTGCCC AGCAGTCAGG AAGAACCCCT GTCCCCACGG 
GACTTGAAAA AATGCTACAG TGTAGAGGCC CAGAGCTGCC GGCGCAGGCC TGGGTCCTGG 
CTAGACGAAC AGAGGAGACA CTCCATCGCT GTCAGCTGCC TGGACAGCGG CTCCCAGCCC 
CGCCTATGTC CAAGCCCCTC AAGCCTCGGG GGCCAACCTC TTGGGGGCCC TGGGAGCCGG 
CCTAAGAAAA AACTCAGCCC ACCCAGTATC TCTATAGACC CCCCGGAGAG CCAGGGCCCT 
CGGCCCCCAT GCAGTCCTGG CGTCTGCCTC AGGAGGAGGG CGCCGGCCAG TGACTCGAAG 
GATCCCTCGG CCTCCAGCCC CCTTGACAGC ACGGCTGCCT CACCCTCCCC AAAGAAAGAT 
GCGCTGAGTC TCTCTGGTTT GTCTTCTGAC CCAACAGACC TGGATCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532080.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532143.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X18, mRNA.

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CloneID	OMu45475
Clone ID Related Accession (Same CDS sequence)	XM_006532080.4 , XM_006532080.3
Accession Version	XM_006532080.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532080.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA AGCCCAGTGC 
AAACCCTACT ACTCTGACTA CTCGCGCTTC CGGCTCCTCG TCCACCACCT GTGTACCAGC 
CACTACCTGG ACCTCTTCAT CACTGGTGTC ATCGGGCTGA ATGTGGTCAC GATGGCCATG 
GAACATTACC AGCAGCCCCA GATCCTGGAC GAGGCTCTGA AGATCTGCAA CTACATCTTT 
ACCGTCATCT TTGTCTTGGA GTCAGTATTC AAACTTGTGG CCTTCGGCTT CCGCCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCTATTGTGC TTCTGTCCAT CATGGGCATC 
ACGCTGGAAG AGATTGAGGT CAATGCTTCA CTGCCCATCA ACCCCACCAT CATCCGTATC 
ATGAGGGTGC TCCGCATTGC TCGAGTTCTG AAGCTGTTGA AGATGGCTGT GGGCATGCGG 
GCACTGCTGG ACACGGTGAT GCAGGCCCTG CCCCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGCTATTAT TTTTCATCTT TGCAGCTCTG GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACAC ACCCTTGTGA GGGCTTGGGC CGGCATGCCA CCTTTAGGAA CTTTGGTATG 
GCCTTTCTGA CCCTCTTCCG AGTCTCCACT GGTGACAACT GGAATGGTAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACC TGCTACAACA CCGTCATCTC ACCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG 
ATGAAGCACC TGGAAGAGAG CAACAAAGAG GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG 
CTGGAGCTAG AGATGAAGAC ACTCAGCCCG CAGCCCCACT CCCCGCTGGG CAGCCCCTTC 
CTCTGGCCTG GGGTGGAAGG TGTCAATAGC CCTGACAGCC CTAAGCCTGG GGCTCCACAC 
ACCACGGCCC ACATTGGAGC AGCCTCTTCA GGCTTCTCCC TTGAGCACCC CACGGACAGA 
CAGCTGTTTG ACACCATCTC CCTGCTGATC CAGGGCTCCC TGGAGGGGGA GCTGAAGCTG 
ATGGACGAGC TGGCAGGCCC AGGGGGCCAG CCCTCTGCCT TCCCTTCCGC CCCCAGCCCG 
GGCGACTCCG ACCCACAGAT GGTACCTCAC ACTGAGGAGG GGCCAGTCCC CCTAGGACCA 
GACCTGCTGA CTGTGAGGAA GTCTGGTGTC AGCCGGACAC ACTCTCTGCC CAATGACAGC 
TACATGTGCC GCAATGGGAG CACTGCCGAG AGATCCCTAG GACACAGGGG CTGGGGGCTC 
CCCAAAGCCC AGTCAGGCTC CATCTTGTCT GTTCACTCCC AACCAGCAGA CACCAGCTGC 
ATCCTACAGC TTCCCAAAGA TGCACACTAT CTGCTCCAGC CTCATGGGGC TCCCACCTGG 
GGCGCCATCC CTAAACTACC CCCACCTGGC CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG 
CGCCAGGCAG CAATAAGGAC TGACTCCCTG GACGTGCAGG GCCTGGGTAG CCGGGAAGAC 
CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC CCTCTGACCC GCTCCTCATC CTTCTGGGGC 
GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC GGCAGCCAGA GCAAAGTCTC CAAGCACATC 
CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA CCCAGCTGGG CCAAGGACCC TCAAGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGCCCAGC 
AGTCAGGAAG AACCCCTGTC CCCACGGGAC TTGAAAAAAT GCTACAGTGT AGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA GACGAACAGA GGAGACACTC CATCGCTGTC 
AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC CTATGTCCAA GCCCCTCAAG CCTCGGGGGC 
CAACCTCTTG GGGGCCCTGG GAGCCGGCCT AAGAAAAAAC TCAGCCCACC CAGTATCTCT 
ATAGACCCCC CGGAGAGCCA GGGCCCTCGG CCCCCATGCA GTCCTGGCGT CTGCCTCAGG 
AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT CCCTCGGCCT CCAGCCCCCT TGACAGCACG 
GCTGCCTCAC CCTCCCCAAA GAAAGATGCG CTGAGTCTCT CTGGTTTGTC TTCTGACCCA 
ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532143.1
CDS	773..7750
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532080.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532080.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA AGCCCAGTGC 
AAACCCTACT ACTCTGACTA CTCGCGCTTC CGGCTCCTCG TCCACCACCT GTGTACCAGC 
CACTACCTGG ACCTCTTCAT CACTGGTGTC ATCGGGCTGA ATGTGGTCAC GATGGCCATG 
GAACATTACC AGCAGCCCCA GATCCTGGAC GAGGCTCTGA AGATCTGCAA CTACATCTTT 
ACCGTCATCT TTGTCTTGGA GTCAGTATTC AAACTTGTGG CCTTCGGCTT CCGCCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCTATTGTGC TTCTGTCCAT CATGGGCATC 
ACGCTGGAAG AGATTGAGGT CAATGCTTCA CTGCCCATCA ACCCCACCAT CATCCGTATC 
ATGAGGGTGC TCCGCATTGC TCGAGTTCTG AAGCTGTTGA AGATGGCTGT GGGCATGCGG 
GCACTGCTGG ACACGGTGAT GCAGGCCCTG CCCCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGCTATTAT TTTTCATCTT TGCAGCTCTG GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACAC ACCCTTGTGA GGGCTTGGGC CGGCATGCCA CCTTTAGGAA CTTTGGTATG 
GCCTTTCTGA CCCTCTTCCG AGTCTCCACT GGTGACAACT GGAATGGTAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACC TGCTACAACA CCGTCATCTC ACCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG 
ATGAAGCACC TGGAAGAGAG CAACAAAGAG GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG 
CTGGAGCTAG AGATGAAGAC ACTCAGCCCG CAGCCCCACT CCCCGCTGGG CAGCCCCTTC 
CTCTGGCCTG GGGTGGAAGG TGTCAATAGC CCTGACAGCC CTAAGCCTGG GGCTCCACAC 
ACCACGGCCC ACATTGGAGC AGCCTCTTCA GGCTTCTCCC TTGAGCACCC CACGGACAGA 
CAGCTGTTTG ACACCATCTC CCTGCTGATC CAGGGCTCCC TGGAGGGGGA GCTGAAGCTG 
ATGGACGAGC TGGCAGGCCC AGGGGGCCAG CCCTCTGCCT TCCCTTCCGC CCCCAGCCCG 
GGCGACTCCG ACCCACAGAT GGTACCTCAC ACTGAGGAGG GGCCAGTCCC CCTAGGACCA 
GACCTGCTGA CTGTGAGGAA GTCTGGTGTC AGCCGGACAC ACTCTCTGCC CAATGACAGC 
TACATGTGCC GCAATGGGAG CACTGCCGAG AGATCCCTAG GACACAGGGG CTGGGGGCTC 
CCCAAAGCCC AGTCAGGCTC CATCTTGTCT GTTCACTCCC AACCAGCAGA CACCAGCTGC 
ATCCTACAGC TTCCCAAAGA TGCACACTAT CTGCTCCAGC CTCATGGGGC TCCCACCTGG 
GGCGCCATCC CTAAACTACC CCCACCTGGC CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG 
CGCCAGGCAG CAATAAGGAC TGACTCCCTG GACGTGCAGG GCCTGGGTAG CCGGGAAGAC 
CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC CCTCTGACCC GCTCCTCATC CTTCTGGGGC 
GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC GGCAGCCAGA GCAAAGTCTC CAAGCACATC 
CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA CCCAGCTGGG CCAAGGACCC TCAAGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGCCCAGC 
AGTCAGGAAG AACCCCTGTC CCCACGGGAC TTGAAAAAAT GCTACAGTGT AGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA GACGAACAGA GGAGACACTC CATCGCTGTC 
AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC CTATGTCCAA GCCCCTCAAG CCTCGGGGGC 
CAACCTCTTG GGGGCCCTGG GAGCCGGCCT AAGAAAAAAC TCAGCCCACC CAGTATCTCT 
ATAGACCCCC CGGAGAGCCA GGGCCCTCGG CCCCCATGCA GTCCTGGCGT CTGCCTCAGG 
AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT CCCTCGGCCT CCAGCCCCCT TGACAGCACG 
GCTGCCTCAC CCTCCCCAAA GAAAGATGCG CTGAGTCTCT CTGGTTTGTC TTCTGACCCA 
ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532081.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532144.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X19, mRNA.

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CloneID	OMu45476
Clone ID Related Accession (Same CDS sequence)	XM_006532081.3 , XM_006532081.4
Accession Version	XM_006532081.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532081.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAA GCCCAGTGCA AACCCTACTA CTCTGACTAC 
TCGCGCTTCC GGCTCCTCGT CCACCACCTG TGTACCAGCC ACTACCTGGA CCTCTTCATC 
ACTGGTGTCA TCGGGCTGAA TGTGGTCACG ATGGCCATGG AACATTACCA GCAGCCCCAG 
ATCCTGGACG AGGCTCTGAA GATCTGCAAC TACATCTTTA CCGTCATCTT TGTCTTGGAG 
TCAGTATTCA AACTTGTGGC CTTCGGCTTC CGCCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CTATTGTGCT TCTGTCCATC ATGGGCATCA CGCTGGAAGA GATTGAGGTC 
AATGCTTCAC TGCCCATCAA CCCCACCATC ATCCGTATCA TGAGGGTGCT CCGCATTGCT 
CGAGTTCTGA AGCTGTTGAA GATGGCTGTG GGCATGCGGG CACTGCTGGA CACGGTGATG 
CAGGCCCTGC CCCAGGTGGG GAACCTGGGA CTTCTCTTCA TGCTATTATT TTTCATCTTT 
GCAGCTCTGG GCGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACACA CCCTTGTGAG 
GGCTTGGGCC GGCATGCCAC CTTTAGGAAC TTTGGTATGG CCTTTCTGAC CCTCTTCCGA 
GTCTCCACTG GTGACAACTG GAATGGTATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACCT GCTACAACAC CGTCATCTCA CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTCATA GCCGTGCTGA TGAAGCACCT GGAAGAGAGC 
AACAAAGAGG CCAAGGAGGA GGCGGAGTTG GAGGCGGAGC TGGAGCTAGA GATGAAGACA 
CTCAGCCCGC AGCCCCACTC CCCGCTGGGC AGCCCCTTCC TCTGGCCTGG GGTGGAAGGT 
GTCAATAGCC CTGACAGCCC TAAGCCTGGG GCTCCACACA CCACGGCCCA CATTGGAGCA 
GCCTCTTCAG GCTTCTCCCT TGAGCACCCC ACGGACAGAC AGCTGTTTGA CACCATCTCC 
CTGCTGATCC AGGGCTCCCT GGAGGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA 
GGGGGCCAGC CCTCTGCCTT CCCTTCCGCC CCCAGCCCGG GCGACTCCGA CCCACAGATG 
GTACCTCACA CTGAGGAGGG GCCAGTCCCC CTAGGACCAG ACCTGCTGAC TGTGAGGAAG 
TCTGGTGTCA GCCGGACACA CTCTCTGCCC AATGACAGCT ACATGTGCCG CAATGGGAGC 
ACTGCCGAGA GATCCCTAGG ACACAGGGGC TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC 
ATCTTGTCTG TTCACTCCCA ACCAGCAGAC ACCAGCTGCA TCCTACAGCT TCCCAAAGAT 
GCACACTATC TGCTCCAGCC TCATGGGGCT CCCACCTGGG GCGCCATCCC TAAACTACCC 
CCACCTGGCC GCTCCCCTCT GGCTCAGAGG CCTCTCAGGC GCCAGGCAGC AATAAGGACT 
GACTCCCTGG ACGTGCAGGG CCTGGGTAGC CGGGAAGACC TGTTGTCAGA GGTGAGTGGG 
CCCTCCTGCC CTCTGACCCG CTCCTCATCC TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG 
CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC AAGCACATCC GCCTGCCAGC CCCTTGCCCA 
GGCCTGGAAC CCAGCTGGGC CAAGGACCCT CAAGAGACCA GAAGCAGCTT AGAGCTGGAC 
ACGGAGCTGA GCTGGATTTC AGGAGACCTC CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC 
CCACGGGACT TGAAAAAATG CTACAGTGTA GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG 
TCCTGGCTAG ACGAACAGAG GAGACACTCC ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC 
CAGCCCCGCC TATGTCCAAG CCCCTCAAGC CTCGGGGGCC AACCTCTTGG GGGCCCTGGG 
AGCCGGCCTA AGAAAAAACT CAGCCCACCC AGTATCTCTA TAGACCCCCC GGAGAGCCAG 
GGCCCTCGGC CCCCATGCAG TCCTGGCGTC TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC 
TCGAAGGATC CCTCGGCCTC CAGCCCCCTT GACAGCACGG CTGCCTCACC CTCCCCAAAG 
AAAGATGCGC TGAGTCTCTC TGGTTTGTCT TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532144.1
CDS	773..7729
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532081.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532081.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAA GCCCAGTGCA AACCCTACTA CTCTGACTAC 
TCGCGCTTCC GGCTCCTCGT CCACCACCTG TGTACCAGCC ACTACCTGGA CCTCTTCATC 
ACTGGTGTCA TCGGGCTGAA TGTGGTCACG ATGGCCATGG AACATTACCA GCAGCCCCAG 
ATCCTGGACG AGGCTCTGAA GATCTGCAAC TACATCTTTA CCGTCATCTT TGTCTTGGAG 
TCAGTATTCA AACTTGTGGC CTTCGGCTTC CGCCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CTATTGTGCT TCTGTCCATC ATGGGCATCA CGCTGGAAGA GATTGAGGTC 
AATGCTTCAC TGCCCATCAA CCCCACCATC ATCCGTATCA TGAGGGTGCT CCGCATTGCT 
CGAGTTCTGA AGCTGTTGAA GATGGCTGTG GGCATGCGGG CACTGCTGGA CACGGTGATG 
CAGGCCCTGC CCCAGGTGGG GAACCTGGGA CTTCTCTTCA TGCTATTATT TTTCATCTTT 
GCAGCTCTGG GCGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACACA CCCTTGTGAG 
GGCTTGGGCC GGCATGCCAC CTTTAGGAAC TTTGGTATGG CCTTTCTGAC CCTCTTCCGA 
GTCTCCACTG GTGACAACTG GAATGGTATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACCT GCTACAACAC CGTCATCTCA CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTCATA GCCGTGCTGA TGAAGCACCT GGAAGAGAGC 
AACAAAGAGG CCAAGGAGGA GGCGGAGTTG GAGGCGGAGC TGGAGCTAGA GATGAAGACA 
CTCAGCCCGC AGCCCCACTC CCCGCTGGGC AGCCCCTTCC TCTGGCCTGG GGTGGAAGGT 
GTCAATAGCC CTGACAGCCC TAAGCCTGGG GCTCCACACA CCACGGCCCA CATTGGAGCA 
GCCTCTTCAG GCTTCTCCCT TGAGCACCCC ACGGACAGAC AGCTGTTTGA CACCATCTCC 
CTGCTGATCC AGGGCTCCCT GGAGGGGGAG CTGAAGCTGA TGGACGAGCT GGCAGGCCCA 
GGGGGCCAGC CCTCTGCCTT CCCTTCCGCC CCCAGCCCGG GCGACTCCGA CCCACAGATG 
GTACCTCACA CTGAGGAGGG GCCAGTCCCC CTAGGACCAG ACCTGCTGAC TGTGAGGAAG 
TCTGGTGTCA GCCGGACACA CTCTCTGCCC AATGACAGCT ACATGTGCCG CAATGGGAGC 
ACTGCCGAGA GATCCCTAGG ACACAGGGGC TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC 
ATCTTGTCTG TTCACTCCCA ACCAGCAGAC ACCAGCTGCA TCCTACAGCT TCCCAAAGAT 
GCACACTATC TGCTCCAGCC TCATGGGGCT CCCACCTGGG GCGCCATCCC TAAACTACCC 
CCACCTGGCC GCTCCCCTCT GGCTCAGAGG CCTCTCAGGC GCCAGGCAGC AATAAGGACT 
GACTCCCTGG ACGTGCAGGG CCTGGGTAGC CGGGAAGACC TGTTGTCAGA GGTGAGTGGG 
CCCTCCTGCC CTCTGACCCG CTCCTCATCC TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG 
CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC AAGCACATCC GCCTGCCAGC CCCTTGCCCA 
GGCCTGGAAC CCAGCTGGGC CAAGGACCCT CAAGAGACCA GAAGCAGCTT AGAGCTGGAC 
ACGGAGCTGA GCTGGATTTC AGGAGACCTC CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC 
CCACGGGACT TGAAAAAATG CTACAGTGTA GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG 
TCCTGGCTAG ACGAACAGAG GAGACACTCC ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC 
CAGCCCCGCC TATGTCCAAG CCCCTCAAGC CTCGGGGGCC AACCTCTTGG GGGCCCTGGG 
AGCCGGCCTA AGAAAAAACT CAGCCCACCC AGTATCTCTA TAGACCCCCC GGAGAGCCAG 
GGCCCTCGGC CCCCATGCAG TCCTGGCGTC TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC 
TCGAAGGATC CCTCGGCCTC CAGCCCCCTT GACAGCACGG CTGCCTCACC CTCCCCAAAG 
AAAGATGCGC TGAGTCTCTC TGGTTTGTCT TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532082.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532145.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X20, mRNA.

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CloneID	OMu45477
Clone ID Related Accession (Same CDS sequence)	XM_006532082.3 , XM_006532082.4
Accession Version	XM_006532082.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532082.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAA TCTAATGTTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCTGCGT CAGAAGCCCA GTGCAAACCC TACTACTCTG ACTACTCGCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGACCTCT TCATCACTGG TGTCATCGGG 
CTGAATGTGG TCACGATGGC CATGGAACAT TACCAGCAGC CCCAGATCCT GGACGAGGCT 
CTGAAGATCT GCAACTACAT CTTTACCGTC ATCTTTGTCT TGGAGTCAGT ATTCAAACTT 
GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCTATT 
GTGCTTCTGT CCATCATGGG CATCACGCTG GAAGAGATTG AGGTCAATGC TTCACTGCCC 
ATCAACCCCA CCATCATCCG TATCATGAGG GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG 
TTGAAGATGG CTGTGGGCAT GCGGGCACTG CTGGACACGG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACC TGGGACTTCT CTTCATGCTA TTATTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGACCTGGA GTGTGATGAG ACACACCCTT GTGAGGGCTT GGGCCGGCAT 
GCCACCTTTA GGAACTTTGG TATGGCCTTT CTGACCCTCT TCCGAGTCTC CACTGGTGAC 
AACTGGAATG GTATTATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACCGTCA TCTCACCCAT CTACTTCGTG TCCTTCGTGC TGACGGCCCA GTTTGTGCTG 
GTCAACGTGG TCATAGCCGT GCTGATGAAG CACCTGGAAG AGAGCAACAA AGAGGCCAAG 
GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG CTAGAGATGA AGACACTCAG CCCGCAGCCC 
CACTCCCCGC TGGGCAGCCC CTTCCTCTGG CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC 
AGCCCTAAGC CTGGGGCTCC ACACACCACG GCCCACATTG GAGCAGCCTC TTCAGGCTTC 
TCCCTTGAGC ACCCCACGAT CCCTCTAGCT GAGATGGAGG CTCTGTCTCT GACGTCAGAG 
ATTGTGTCTG AACCGTCCTG CTCTCTAGCT CTGACGGATG ACTCTTTGCC TGATGACACT 
CACACACTCT TACTTAGTGC CCTGGAGAGC AATATGGTAC CTCACACTGA GGAGGGGCCA 
GTCCCCCTAG GACCAGACCT GCTGACTGTG AGGAAGTCTG GTGTCAGCCG GACACACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGCAAT GGGAGCACTG CCGAGAGATC CCTAGGACAC 
AGGGGCTGGG GGCTCCCCAA AGCCCAGTCA GGCTCCATCT TGTCTGTTCA CTCCCAACCA 
GCAGACACCA GCTGCATCCT ACAGCTTCCC AAAGATGCAC ACTATCTGCT CCAGCCTCAT 
GGGGCTCCCA CCTGGGGCGC CATCCCTAAA CTACCCCCAC CTGGCCGCTC CCCTCTGGCT 
CAGAGGCCTC TCAGGCGCCA GGCAGCAATA AGGACTGACT CCCTGGACGT GCAGGGCCTG 
GGTAGCCGGG AAGACCTGTT GTCAGAGGTG AGTGGGCCCT CCTGCCCTCT GACCCGCTCC 
TCATCCTTCT GGGGCGGGTC GAGCATCCAG GTGCAGCAGC GCTCCGGCAG CCAGAGCAAA 
GTCTCCAAGC ACATCCGCCT GCCAGCCCCT TGCCCAGGCC TGGAACCCAG CTGGGCCAAG 
GACCCTCAAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCAGCAGTCA GGAAGAACCC CTGTCCCCAC GGGACTTGAA AAAATGCTAC 
AGTGTAGAGG CCCAGAGCTG CCGGCGCAGG CCTGGGTCCT GGCTAGACGA ACAGAGGAGA 
CACTCCATCG CTGTCAGCTG CCTGGACAGC GGCTCCCAGC CCCGCCTATG TCCAAGCCCC 
TCAAGCCTCG GGGGCCAACC TCTTGGGGGC CCTGGGAGCC GGCCTAAGAA AAAACTCAGC 
CCACCCAGTA TCTCTATAGA CCCCCCGGAG AGCCAGGGCC CTCGGCCCCC ATGCAGTCCT 
GGCGTCTGCC TCAGGAGGAG GGCGCCGGCC AGTGACTCGA AGGATCCCTC GGCCTCCAGC 
CCCCTTGACA GCACGGCTGC CTCACCCTCC CCAAAGAAAG ATGCGCTGAG TCTCTCTGGT 
TTGTCTTCTG ACCCAACAGA CCTGGATCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532145.1
CDS	773..7705
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532082.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532082.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAA TCTAATGTTG GACGATGTAA TTGCTTCCGG CAGCTCAGCC 
AGCGCTGCGT CAGAAGCCCA GTGCAAACCC TACTACTCTG ACTACTCGCG CTTCCGGCTC 
CTCGTCCACC ACCTGTGTAC CAGCCACTAC CTGGACCTCT TCATCACTGG TGTCATCGGG 
CTGAATGTGG TCACGATGGC CATGGAACAT TACCAGCAGC CCCAGATCCT GGACGAGGCT 
CTGAAGATCT GCAACTACAT CTTTACCGTC ATCTTTGTCT TGGAGTCAGT ATTCAAACTT 
GTGGCCTTCG GCTTCCGCCG GTTCTTCCAG GACAGGTGGA ACCAGCTGGA CCTGGCTATT 
GTGCTTCTGT CCATCATGGG CATCACGCTG GAAGAGATTG AGGTCAATGC TTCACTGCCC 
ATCAACCCCA CCATCATCCG TATCATGAGG GTGCTCCGCA TTGCTCGAGT TCTGAAGCTG 
TTGAAGATGG CTGTGGGCAT GCGGGCACTG CTGGACACGG TGATGCAGGC CCTGCCCCAG 
GTGGGGAACC TGGGACTTCT CTTCATGCTA TTATTTTTCA TCTTTGCAGC TCTGGGCGTG 
GAGCTCTTTG GAGACCTGGA GTGTGATGAG ACACACCCTT GTGAGGGCTT GGGCCGGCAT 
GCCACCTTTA GGAACTTTGG TATGGCCTTT CTGACCCTCT TCCGAGTCTC CACTGGTGAC 
AACTGGAATG GTATTATGAA GGACACCCTC CGGGACTGTG ACCAGGAGTC CACCTGCTAC 
AACACCGTCA TCTCACCCAT CTACTTCGTG TCCTTCGTGC TGACGGCCCA GTTTGTGCTG 
GTCAACGTGG TCATAGCCGT GCTGATGAAG CACCTGGAAG AGAGCAACAA AGAGGCCAAG 
GAGGAGGCGG AGTTGGAGGC GGAGCTGGAG CTAGAGATGA AGACACTCAG CCCGCAGCCC 
CACTCCCCGC TGGGCAGCCC CTTCCTCTGG CCTGGGGTGG AAGGTGTCAA TAGCCCTGAC 
AGCCCTAAGC CTGGGGCTCC ACACACCACG GCCCACATTG GAGCAGCCTC TTCAGGCTTC 
TCCCTTGAGC ACCCCACGAT CCCTCTAGCT GAGATGGAGG CTCTGTCTCT GACGTCAGAG 
ATTGTGTCTG AACCGTCCTG CTCTCTAGCT CTGACGGATG ACTCTTTGCC TGATGACACT 
CACACACTCT TACTTAGTGC CCTGGAGAGC AATATGGTAC CTCACACTGA GGAGGGGCCA 
GTCCCCCTAG GACCAGACCT GCTGACTGTG AGGAAGTCTG GTGTCAGCCG GACACACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGCAAT GGGAGCACTG CCGAGAGATC CCTAGGACAC 
AGGGGCTGGG GGCTCCCCAA AGCCCAGTCA GGCTCCATCT TGTCTGTTCA CTCCCAACCA 
GCAGACACCA GCTGCATCCT ACAGCTTCCC AAAGATGCAC ACTATCTGCT CCAGCCTCAT 
GGGGCTCCCA CCTGGGGCGC CATCCCTAAA CTACCCCCAC CTGGCCGCTC CCCTCTGGCT 
CAGAGGCCTC TCAGGCGCCA GGCAGCAATA AGGACTGACT CCCTGGACGT GCAGGGCCTG 
GGTAGCCGGG AAGACCTGTT GTCAGAGGTG AGTGGGCCCT CCTGCCCTCT GACCCGCTCC 
TCATCCTTCT GGGGCGGGTC GAGCATCCAG GTGCAGCAGC GCTCCGGCAG CCAGAGCAAA 
GTCTCCAAGC ACATCCGCCT GCCAGCCCCT TGCCCAGGCC TGGAACCCAG CTGGGCCAAG 
GACCCTCAAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCAGCAGTCA GGAAGAACCC CTGTCCCCAC GGGACTTGAA AAAATGCTAC 
AGTGTAGAGG CCCAGAGCTG CCGGCGCAGG CCTGGGTCCT GGCTAGACGA ACAGAGGAGA 
CACTCCATCG CTGTCAGCTG CCTGGACAGC GGCTCCCAGC CCCGCCTATG TCCAAGCCCC 
TCAAGCCTCG GGGGCCAACC TCTTGGGGGC CCTGGGAGCC GGCCTAAGAA AAAACTCAGC 
CCACCCAGTA TCTCTATAGA CCCCCCGGAG AGCCAGGGCC CTCGGCCCCC ATGCAGTCCT 
GGCGTCTGCC TCAGGAGGAG GGCGCCGGCC AGTGACTCGA AGGATCCCTC GGCCTCCAGC 
CCCCTTGACA GCACGGCTGC CTCACCCTCC CCAAAGAAAG ATGCGCTGAG TCTCTCTGGT 
TTGTCTTCTG ACCCAACAGA CCTGGATCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532083.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532146.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X21, mRNA.

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CloneID	OMu45478
Clone ID Related Accession (Same CDS sequence)	XM_006532083.3 , XM_006532083.4
Accession Version	XM_006532083.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532083.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGGACAG ACAGCTGTTT 
GACACCATCT CCCTGCTGAT CCAGGGCTCC CTGGAGGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTTCCG CCCCCAGCCC GGGCGACTCC 
GACCCACAGA TGGTACCTCA CACTGAGGAG GGGCCAGTCC CCCTAGGACC AGACCTGCTG 
ACTGTGAGGA AGTCTGGTGT CAGCCGGACA CACTCTCTGC CCAATGACAG CTACATGTGC 
CGCAATGGGA GCACTGCCGA GAGATCCCTA GGACACAGGG GCTGGGGGCT CCCCAAAGCC 
CAGTCAGGCT CCATCTTGTC TGTTCACTCC CAACCAGCAG ACACCAGCTG CATCCTACAG 
CTTCCCAAAG ATGCACACTA TCTGCTCCAG CCTCATGGGG CTCCCACCTG GGGCGCCATC 
CCTAAACTAC CCCCACCTGG CCGCTCCCCT CTGGCTCAGA GGCCTCTCAG GCGCCAGGCA 
GCAATAAGGA CTGACTCCCT GGACGTGCAG GGCCTGGGTA GCCGGGAAGA CCTGTTGTCA 
GAGGTGAGTG GGCCCTCCTG CCCTCTGACC CGCTCCTCAT CCTTCTGGGG CGGGTCGAGC 
ATCCAGGTGC AGCAGCGCTC CGGCAGCCAG AGCAAAGTCT CCAAGCACAT CCGCCTGCCA 
GCCCCTTGCC CAGGCCTGGA ACCCAGCTGG GCCAAGGACC CTCAAGAGAC CAGAAGCAGC 
TTAGAGCTGG ACACGGAGCT GAGCTGGATT TCAGGAGACC TCCTGCCCAG CAGTCAGGAA 
GAACCCCTGT CCCCACGGGA CTTGAAAAAA TGCTACAGTG TAGAGGCCCA GAGCTGCCGG 
CGCAGGCCTG GGTCCTGGCT AGACGAACAG AGGAGACACT CCATCGCTGT CAGCTGCCTG 
GACAGCGGCT CCCAGCCCCG CCTATGTCCA AGCCCCTCAA GCCTCGGGGG CCAACCTCTT 
GGGGGCCCTG GGAGCCGGCC TAAGAAAAAA CTCAGCCCAC CCAGTATCTC TATAGACCCC 
CCGGAGAGCC AGGGCCCTCG GCCCCCATGC AGTCCTGGCG TCTGCCTCAG GAGGAGGGCG 
CCGGCCAGTG ACTCGAAGGA TCCCTCGGCC TCCAGCCCCC TTGACAGCAC GGCTGCCTCA 
CCCTCCCCAA AGAAAGATGC GCTGAGTCTC TCTGGTTTGT CTTCTGACCC AACAGACCTG 
GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532146.1
CDS	773..7681
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532083.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532083.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGGACAG ACAGCTGTTT 
GACACCATCT CCCTGCTGAT CCAGGGCTCC CTGGAGGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTTCCG CCCCCAGCCC GGGCGACTCC 
GACCCACAGA TGGTACCTCA CACTGAGGAG GGGCCAGTCC CCCTAGGACC AGACCTGCTG 
ACTGTGAGGA AGTCTGGTGT CAGCCGGACA CACTCTCTGC CCAATGACAG CTACATGTGC 
CGCAATGGGA GCACTGCCGA GAGATCCCTA GGACACAGGG GCTGGGGGCT CCCCAAAGCC 
CAGTCAGGCT CCATCTTGTC TGTTCACTCC CAACCAGCAG ACACCAGCTG CATCCTACAG 
CTTCCCAAAG ATGCACACTA TCTGCTCCAG CCTCATGGGG CTCCCACCTG GGGCGCCATC 
CCTAAACTAC CCCCACCTGG CCGCTCCCCT CTGGCTCAGA GGCCTCTCAG GCGCCAGGCA 
GCAATAAGGA CTGACTCCCT GGACGTGCAG GGCCTGGGTA GCCGGGAAGA CCTGTTGTCA 
GAGGTGAGTG GGCCCTCCTG CCCTCTGACC CGCTCCTCAT CCTTCTGGGG CGGGTCGAGC 
ATCCAGGTGC AGCAGCGCTC CGGCAGCCAG AGCAAAGTCT CCAAGCACAT CCGCCTGCCA 
GCCCCTTGCC CAGGCCTGGA ACCCAGCTGG GCCAAGGACC CTCAAGAGAC CAGAAGCAGC 
TTAGAGCTGG ACACGGAGCT GAGCTGGATT TCAGGAGACC TCCTGCCCAG CAGTCAGGAA 
GAACCCCTGT CCCCACGGGA CTTGAAAAAA TGCTACAGTG TAGAGGCCCA GAGCTGCCGG 
CGCAGGCCTG GGTCCTGGCT AGACGAACAG AGGAGACACT CCATCGCTGT CAGCTGCCTG 
GACAGCGGCT CCCAGCCCCG CCTATGTCCA AGCCCCTCAA GCCTCGGGGG CCAACCTCTT 
GGGGGCCCTG GGAGCCGGCC TAAGAAAAAA CTCAGCCCAC CCAGTATCTC TATAGACCCC 
CCGGAGAGCC AGGGCCCTCG GCCCCCATGC AGTCCTGGCG TCTGCCTCAG GAGGAGGGCG 
CCGGCCAGTG ACTCGAAGGA TCCCTCGGCC TCCAGCCCCC TTGACAGCAC GGCTGCCTCA 
CCCTCCCCAA AGAAAGATGC GCTGAGTCTC TCTGGTTTGT CTTCTGACCC AACAGACCTG 
GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532084.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532147.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X24, mRNA.

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CloneID	OMu45479
Clone ID Related Accession (Same CDS sequence)	XM_006532084.4 , XM_006532084.3
Accession Version	XM_006532084.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532084.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAT CTAATGTTGG ACGATGTAAT TGCTTCCGGC 
AGCTCAGCCA GCGCTGCGTC AGAAGCCCAG TGCAAACCCT ACTACTCTGA CTACTCGCGC 
TTCCGGCTCC TCGTCCACCA CCTGTGTACC AGCCACTACC TGGACCTCTT CATCACTGGT 
GTCATCGGGC TGAATGTGGT CACGATGGCC ATGGAACATT ACCAGCAGCC CCAGATCCTG 
GACGAGGCTC TGAAGATCTG CAACTACATC TTTACCGTCA TCTTTGTCTT GGAGTCAGTA 
TTCAAACTTG TGGCCTTCGG CTTCCGCCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCTATTG TGCTTCTGTC CATCATGGGC ATCACGCTGG AAGAGATTGA GGTCAATGCT 
TCACTGCCCA TCAACCCCAC CATCATCCGT ATCATGAGGG TGCTCCGCAT TGCTCGAGTT 
CTGAAGCTGT TGAAGATGGC TGTGGGCATG CGGGCACTGC TGGACACGGT GATGCAGGCC 
CTGCCCCAGG TGGGGAACCT GGGACTTCTC TTCATGCTAT TATTTTTCAT CTTTGCAGCT 
CTGGGCGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CACACCCTTG TGAGGGCTTG 
GGCCGGCATG CCACCTTTAG GAACTTTGGT ATGGCCTTTC TGACCCTCTT CCGAGTCTCC 
ACTGGTGACA ACTGGAATGG TATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACCTGCTACA ACACCGTCAT CTCACCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT CATAGCCGTG CTGATGAAGC ACCTGGAAGA GAGCAACAAA 
GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG GAGCTGGAGC TAGAGATGAA GACACTCAGC 
CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT 
AGCCCTGACA GCCCTAAGCC TGGGGCTCCA CACACCACGG CCCACATTGG AGCAGCCTCT 
TCAGGCTTCT CCCTTGAGCA CCCCACGATG GTACCTCACA CTGAGGAGGG GCCAGTCCCC 
CTAGGACCAG ACCTGCTGAC TGTGAGGAAG TCTGGTGTCA GCCGGACACA CTCTCTGCCC 
AATGACAGCT ACATGTGCCG CAATGGGAGC ACTGCCGAGA GATCCCTAGG ACACAGGGGC 
TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC ATCTTGTCTG TTCACTCCCA ACCAGCAGAC 
ACCAGCTGCA TCCTACAGCT TCCCAAAGAT GCACACTATC TGCTCCAGCC TCATGGGGCT 
CCCACCTGGG GCGCCATCCC TAAACTACCC CCACCTGGCC GCTCCCCTCT GGCTCAGAGG 
CCTCTCAGGC GCCAGGCAGC AATAAGGACT GACTCCCTGG ACGTGCAGGG CCTGGGTAGC 
CGGGAAGACC TGTTGTCAGA GGTGAGTGGG CCCTCCTGCC CTCTGACCCG CTCCTCATCC 
TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC 
AAGCACATCC GCCTGCCAGC CCCTTGCCCA GGCCTGGAAC CCAGCTGGGC CAAGGACCCT 
CAAGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC CCACGGGACT TGAAAAAATG CTACAGTGTA 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG TCCTGGCTAG ACGAACAGAG GAGACACTCC 
ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC CAGCCCCGCC TATGTCCAAG CCCCTCAAGC 
CTCGGGGGCC AACCTCTTGG GGGCCCTGGG AGCCGGCCTA AGAAAAAACT CAGCCCACCC 
AGTATCTCTA TAGACCCCCC GGAGAGCCAG GGCCCTCGGC CCCCATGCAG TCCTGGCGTC 
TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC TCGAAGGATC CCTCGGCCTC CAGCCCCCTT 
GACAGCACGG CTGCCTCACC CTCCCCAAAG AAAGATGCGC TGAGTCTCTC TGGTTTGTCT 
TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532147.1
CDS	773..7639
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532084.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532084.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAT CTAATGTTGG ACGATGTAAT TGCTTCCGGC 
AGCTCAGCCA GCGCTGCGTC AGAAGCCCAG TGCAAACCCT ACTACTCTGA CTACTCGCGC 
TTCCGGCTCC TCGTCCACCA CCTGTGTACC AGCCACTACC TGGACCTCTT CATCACTGGT 
GTCATCGGGC TGAATGTGGT CACGATGGCC ATGGAACATT ACCAGCAGCC CCAGATCCTG 
GACGAGGCTC TGAAGATCTG CAACTACATC TTTACCGTCA TCTTTGTCTT GGAGTCAGTA 
TTCAAACTTG TGGCCTTCGG CTTCCGCCGG TTCTTCCAGG ACAGGTGGAA CCAGCTGGAC 
CTGGCTATTG TGCTTCTGTC CATCATGGGC ATCACGCTGG AAGAGATTGA GGTCAATGCT 
TCACTGCCCA TCAACCCCAC CATCATCCGT ATCATGAGGG TGCTCCGCAT TGCTCGAGTT 
CTGAAGCTGT TGAAGATGGC TGTGGGCATG CGGGCACTGC TGGACACGGT GATGCAGGCC 
CTGCCCCAGG TGGGGAACCT GGGACTTCTC TTCATGCTAT TATTTTTCAT CTTTGCAGCT 
CTGGGCGTGG AGCTCTTTGG AGACCTGGAG TGTGATGAGA CACACCCTTG TGAGGGCTTG 
GGCCGGCATG CCACCTTTAG GAACTTTGGT ATGGCCTTTC TGACCCTCTT CCGAGTCTCC 
ACTGGTGACA ACTGGAATGG TATTATGAAG GACACCCTCC GGGACTGTGA CCAGGAGTCC 
ACCTGCTACA ACACCGTCAT CTCACCCATC TACTTCGTGT CCTTCGTGCT GACGGCCCAG 
TTTGTGCTGG TCAACGTGGT CATAGCCGTG CTGATGAAGC ACCTGGAAGA GAGCAACAAA 
GAGGCCAAGG AGGAGGCGGA GTTGGAGGCG GAGCTGGAGC TAGAGATGAA GACACTCAGC 
CCGCAGCCCC ACTCCCCGCT GGGCAGCCCC TTCCTCTGGC CTGGGGTGGA AGGTGTCAAT 
AGCCCTGACA GCCCTAAGCC TGGGGCTCCA CACACCACGG CCCACATTGG AGCAGCCTCT 
TCAGGCTTCT CCCTTGAGCA CCCCACGATG GTACCTCACA CTGAGGAGGG GCCAGTCCCC 
CTAGGACCAG ACCTGCTGAC TGTGAGGAAG TCTGGTGTCA GCCGGACACA CTCTCTGCCC 
AATGACAGCT ACATGTGCCG CAATGGGAGC ACTGCCGAGA GATCCCTAGG ACACAGGGGC 
TGGGGGCTCC CCAAAGCCCA GTCAGGCTCC ATCTTGTCTG TTCACTCCCA ACCAGCAGAC 
ACCAGCTGCA TCCTACAGCT TCCCAAAGAT GCACACTATC TGCTCCAGCC TCATGGGGCT 
CCCACCTGGG GCGCCATCCC TAAACTACCC CCACCTGGCC GCTCCCCTCT GGCTCAGAGG 
CCTCTCAGGC GCCAGGCAGC AATAAGGACT GACTCCCTGG ACGTGCAGGG CCTGGGTAGC 
CGGGAAGACC TGTTGTCAGA GGTGAGTGGG CCCTCCTGCC CTCTGACCCG CTCCTCATCC 
TTCTGGGGCG GGTCGAGCAT CCAGGTGCAG CAGCGCTCCG GCAGCCAGAG CAAAGTCTCC 
AAGCACATCC GCCTGCCAGC CCCTTGCCCA GGCCTGGAAC CCAGCTGGGC CAAGGACCCT 
CAAGAGACCA GAAGCAGCTT AGAGCTGGAC ACGGAGCTGA GCTGGATTTC AGGAGACCTC 
CTGCCCAGCA GTCAGGAAGA ACCCCTGTCC CCACGGGACT TGAAAAAATG CTACAGTGTA 
GAGGCCCAGA GCTGCCGGCG CAGGCCTGGG TCCTGGCTAG ACGAACAGAG GAGACACTCC 
ATCGCTGTCA GCTGCCTGGA CAGCGGCTCC CAGCCCCGCC TATGTCCAAG CCCCTCAAGC 
CTCGGGGGCC AACCTCTTGG GGGCCCTGGG AGCCGGCCTA AGAAAAAACT CAGCCCACCC 
AGTATCTCTA TAGACCCCCC GGAGAGCCAG GGCCCTCGGC CCCCATGCAG TCCTGGCGTC 
TGCCTCAGGA GGAGGGCGCC GGCCAGTGAC TCGAAGGATC CCTCGGCCTC CAGCCCCCTT 
GACAGCACGG CTGCCTCACC CTCCCCAAAG AAAGATGCGC TGAGTCTCTC TGGTTTGTCT 
TCTGACCCAA CAGACCTGGA TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532085.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532148.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X26, mRNA.

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CloneID	OMu45480
Clone ID Related Accession (Same CDS sequence)	XM_006532085.3 , XM_006532085.4
Accession Version	XM_006532085.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532085.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA AGCCCAGTGC 
AAACCCTACT ACTCTGACTA CTCGCGCTTC CGGCTCCTCG TCCACCACCT GTGTACCAGC 
CACTACCTGG ACCTCTTCAT CACTGGTGTC ATCGGGCTGA ATGTGGTCAC GATGGCCATG 
GAACATTACC AGCAGCCCCA GATCCTGGAC GAGGCTCTGA AGATCTGCAA CTACATCTTT 
ACCGTCATCT TTGTCTTGGA GTCAGTATTC AAACTTGTGG CCTTCGGCTT CCGCCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCTATTGTGC TTCTGTCCAT CATGGGCATC 
ACGCTGGAAG AGATTGAGGT CAATGCTTCA CTGCCCATCA ACCCCACCAT CATCCGTATC 
ATGAGGGTGC TCCGCATTGC TCGAGTTCTG AAGCTGTTGA AGATGGCTGT GGGCATGCGG 
GCACTGCTGG ACACGGTGAT GCAGGCCCTG CCCCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGCTATTAT TTTTCATCTT TGCAGCTCTG GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACAC ACCCTTGTGA GGGCTTGGGC CGGCATGCCA CCTTTAGGAA CTTTGGTATG 
GCCTTTCTGA CCCTCTTCCG AGTCTCCACT GGTGACAACT GGAATGGTAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACC TGCTACAACA CCGTCATCTC ACCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG 
ATGAAGCACC TGGAAGAGAG CAACAAAGAG GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG 
CTGGAGCTAG AGATGAAGAC ACTCAGCCCG CAGCCCCACT CCCCGCTGGG CAGCCCCTTC 
CTCTGGCCTG GGGTGGAAGG TGTCAATAGC CCTGACAGCC CTAAGCCTGG GGCTCCACAC 
ACCACGGCCC ACATTGGAGC AGCCTCTTCA GGCTTCTCCC TTGAGCACCC CACGATGGTA 
CCTCACACTG AGGAGGGGCC AGTCCCCCTA GGACCAGACC TGCTGACTGT GAGGAAGTCT 
GGTGTCAGCC GGACACACTC TCTGCCCAAT GACAGCTACA TGTGCCGCAA TGGGAGCACT 
GCCGAGAGAT CCCTAGGACA CAGGGGCTGG GGGCTCCCCA AAGCCCAGTC AGGCTCCATC 
TTGTCTGTTC ACTCCCAACC AGCAGACACC AGCTGCATCC TACAGCTTCC CAAAGATGCA 
CACTATCTGC TCCAGCCTCA TGGGGCTCCC ACCTGGGGCG CCATCCCTAA ACTACCCCCA 
CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT CTCAGGCGCC AGGCAGCAAT AAGGACTGAC 
TCCCTGGACG TGCAGGGCCT GGGTAGCCGG GAAGACCTGT TGTCAGAGGT GAGTGGGCCC 
TCCTGCCCTC TGACCCGCTC CTCATCCTTC TGGGGCGGGT CGAGCATCCA GGTGCAGCAG 
CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG CACATCCGCC TGCCAGCCCC TTGCCCAGGC 
CTGGAACCCA GCTGGGCCAA GGACCCTCAA GAGACCAGAA GCAGCTTAGA GCTGGACACG 
GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCAGCAGTC AGGAAGAACC CCTGTCCCCA 
CGGGACTTGA AAAAATGCTA CAGTGTAGAG GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC 
TGGCTAGACG AACAGAGGAG ACACTCCATC GCTGTCAGCT GCCTGGACAG CGGCTCCCAG 
CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC 
CGGCCTAAGA AAAAACTCAG CCCACCCAGT ATCTCTATAG ACCCCCCGGA GAGCCAGGGC 
CCTCGGCCCC CATGCAGTCC TGGCGTCTGC CTCAGGAGGA GGGCGCCGGC CAGTGACTCG 
AAGGATCCCT CGGCCTCCAG CCCCCTTGAC AGCACGGCTG CCTCACCCTC CCCAAAGAAA 
GATGCGCTGA GTCTCTCTGG TTTGTCTTCT GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532148.1
CDS	773..7606
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532085.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532085.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAGT AAGGAGAAGC AGATGGCCGA AGCCCAGTGC 
AAACCCTACT ACTCTGACTA CTCGCGCTTC CGGCTCCTCG TCCACCACCT GTGTACCAGC 
CACTACCTGG ACCTCTTCAT CACTGGTGTC ATCGGGCTGA ATGTGGTCAC GATGGCCATG 
GAACATTACC AGCAGCCCCA GATCCTGGAC GAGGCTCTGA AGATCTGCAA CTACATCTTT 
ACCGTCATCT TTGTCTTGGA GTCAGTATTC AAACTTGTGG CCTTCGGCTT CCGCCGGTTC 
TTCCAGGACA GGTGGAACCA GCTGGACCTG GCTATTGTGC TTCTGTCCAT CATGGGCATC 
ACGCTGGAAG AGATTGAGGT CAATGCTTCA CTGCCCATCA ACCCCACCAT CATCCGTATC 
ATGAGGGTGC TCCGCATTGC TCGAGTTCTG AAGCTGTTGA AGATGGCTGT GGGCATGCGG 
GCACTGCTGG ACACGGTGAT GCAGGCCCTG CCCCAGGTGG GGAACCTGGG ACTTCTCTTC 
ATGCTATTAT TTTTCATCTT TGCAGCTCTG GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT 
GATGAGACAC ACCCTTGTGA GGGCTTGGGC CGGCATGCCA CCTTTAGGAA CTTTGGTATG 
GCCTTTCTGA CCCTCTTCCG AGTCTCCACT GGTGACAACT GGAATGGTAT TATGAAGGAC 
ACCCTCCGGG ACTGTGACCA GGAGTCCACC TGCTACAACA CCGTCATCTC ACCCATCTAC 
TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG 
ATGAAGCACC TGGAAGAGAG CAACAAAGAG GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG 
CTGGAGCTAG AGATGAAGAC ACTCAGCCCG CAGCCCCACT CCCCGCTGGG CAGCCCCTTC 
CTCTGGCCTG GGGTGGAAGG TGTCAATAGC CCTGACAGCC CTAAGCCTGG GGCTCCACAC 
ACCACGGCCC ACATTGGAGC AGCCTCTTCA GGCTTCTCCC TTGAGCACCC CACGATGGTA 
CCTCACACTG AGGAGGGGCC AGTCCCCCTA GGACCAGACC TGCTGACTGT GAGGAAGTCT 
GGTGTCAGCC GGACACACTC TCTGCCCAAT GACAGCTACA TGTGCCGCAA TGGGAGCACT 
GCCGAGAGAT CCCTAGGACA CAGGGGCTGG GGGCTCCCCA AAGCCCAGTC AGGCTCCATC 
TTGTCTGTTC ACTCCCAACC AGCAGACACC AGCTGCATCC TACAGCTTCC CAAAGATGCA 
CACTATCTGC TCCAGCCTCA TGGGGCTCCC ACCTGGGGCG CCATCCCTAA ACTACCCCCA 
CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT CTCAGGCGCC AGGCAGCAAT AAGGACTGAC 
TCCCTGGACG TGCAGGGCCT GGGTAGCCGG GAAGACCTGT TGTCAGAGGT GAGTGGGCCC 
TCCTGCCCTC TGACCCGCTC CTCATCCTTC TGGGGCGGGT CGAGCATCCA GGTGCAGCAG 
CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG CACATCCGCC TGCCAGCCCC TTGCCCAGGC 
CTGGAACCCA GCTGGGCCAA GGACCCTCAA GAGACCAGAA GCAGCTTAGA GCTGGACACG 
GAGCTGAGCT GGATTTCAGG AGACCTCCTG CCCAGCAGTC AGGAAGAACC CCTGTCCCCA 
CGGGACTTGA AAAAATGCTA CAGTGTAGAG GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC 
TGGCTAGACG AACAGAGGAG ACACTCCATC GCTGTCAGCT GCCTGGACAG CGGCTCCCAG 
CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC 
CGGCCTAAGA AAAAACTCAG CCCACCCAGT ATCTCTATAG ACCCCCCGGA GAGCCAGGGC 
CCTCGGCCCC CATGCAGTCC TGGCGTCTGC CTCAGGAGGA GGGCGCCGGC CAGTGACTCG 
AAGGATCCCT CGGCCTCCAG CCCCCTTGAC AGCACGGCTG CCTCACCCTC CCCAAAGAAA 
GATGCGCTGA GTCTCTCTGG TTTGTCTTCT GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532086.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532149.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X28, mRNA.

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CloneID	OMu45481
Clone ID Related Accession (Same CDS sequence)	XM_006532086.3 , XM_006532086.4
Accession Version	XM_006532086.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532086.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAA GCCCAGTGCA AACCCTACTA CTCTGACTAC 
TCGCGCTTCC GGCTCCTCGT CCACCACCTG TGTACCAGCC ACTACCTGGA CCTCTTCATC 
ACTGGTGTCA TCGGGCTGAA TGTGGTCACG ATGGCCATGG AACATTACCA GCAGCCCCAG 
ATCCTGGACG AGGCTCTGAA GATCTGCAAC TACATCTTTA CCGTCATCTT TGTCTTGGAG 
TCAGTATTCA AACTTGTGGC CTTCGGCTTC CGCCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CTATTGTGCT TCTGTCCATC ATGGGCATCA CGCTGGAAGA GATTGAGGTC 
AATGCTTCAC TGCCCATCAA CCCCACCATC ATCCGTATCA TGAGGGTGCT CCGCATTGCT 
CGAGTTCTGA AGCTGTTGAA GATGGCTGTG GGCATGCGGG CACTGCTGGA CACGGTGATG 
CAGGCCCTGC CCCAGGTGGG GAACCTGGGA CTTCTCTTCA TGCTATTATT TTTCATCTTT 
GCAGCTCTGG GCGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACACA CCCTTGTGAG 
GGCTTGGGCC GGCATGCCAC CTTTAGGAAC TTTGGTATGG CCTTTCTGAC CCTCTTCCGA 
GTCTCCACTG GTGACAACTG GAATGGTATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACCT GCTACAACAC CGTCATCTCA CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTCATA GCCGTGCTGA TGAAGCACCT GGAAGAGAGC 
AACAAAGAGG CCAAGGAGGA GGCGGAGTTG GAGGCGGAGC TGGAGCTAGA GATGAAGACA 
CTCAGCCCGC AGCCCCACTC CCCGCTGGGC AGCCCCTTCC TCTGGCCTGG GGTGGAAGGT 
GTCAATAGCC CTGACAGCCC TAAGCCTGGG GCTCCACACA CCACGGCCCA CATTGGAGCA 
GCCTCTTCAG GCTTCTCCCT TGAGCACCCC ACGATGGTAC CTCACACTGA GGAGGGGCCA 
GTCCCCCTAG GACCAGACCT GCTGACTGTG AGGAAGTCTG GTGTCAGCCG GACACACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGCAAT GGGAGCACTG CCGAGAGATC CCTAGGACAC 
AGGGGCTGGG GGCTCCCCAA AGCCCAGTCA GGCTCCATCT TGTCTGTTCA CTCCCAACCA 
GCAGACACCA GCTGCATCCT ACAGCTTCCC AAAGATGCAC ACTATCTGCT CCAGCCTCAT 
GGGGCTCCCA CCTGGGGCGC CATCCCTAAA CTACCCCCAC CTGGCCGCTC CCCTCTGGCT 
CAGAGGCCTC TCAGGCGCCA GGCAGCAATA AGGACTGACT CCCTGGACGT GCAGGGCCTG 
GGTAGCCGGG AAGACCTGTT GTCAGAGGTG AGTGGGCCCT CCTGCCCTCT GACCCGCTCC 
TCATCCTTCT GGGGCGGGTC GAGCATCCAG GTGCAGCAGC GCTCCGGCAG CCAGAGCAAA 
GTCTCCAAGC ACATCCGCCT GCCAGCCCCT TGCCCAGGCC TGGAACCCAG CTGGGCCAAG 
GACCCTCAAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCAGCAGTCA GGAAGAACCC CTGTCCCCAC GGGACTTGAA AAAATGCTAC 
AGTGTAGAGG CCCAGAGCTG CCGGCGCAGG CCTGGGTCCT GGCTAGACGA ACAGAGGAGA 
CACTCCATCG CTGTCAGCTG CCTGGACAGC GGCTCCCAGC CCCGCCTATG TCCAAGCCCC 
TCAAGCCTCG GGGGCCAACC TCTTGGGGGC CCTGGGAGCC GGCCTAAGAA AAAACTCAGC 
CCACCCAGTA TCTCTATAGA CCCCCCGGAG AGCCAGGGCC CTCGGCCCCC ATGCAGTCCT 
GGCGTCTGCC TCAGGAGGAG GGCGCCGGCC AGTGACTCGA AGGATCCCTC GGCCTCCAGC 
CCCCTTGACA GCACGGCTGC CTCACCCTCC CCAAAGAAAG ATGCGCTGAG TCTCTCTGGT 
TTGTCTTCTG ACCCAACAGA CCTGGATCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532149.1
CDS	773..7585
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532086.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532086.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GAAATCGGCA AACGGGAAGA TACGAGTGGA 
CAGTTAAGCT GTATTCAGCT GCCTGTCAAC TCCCAGGGGG GAGATGCCAC CAAGTCTGAG 
TCAGAGCCTG ATTTCTTTTC GCCCAGTGTG GATGGTGATG GGGACAGGAA GAAGCGCTTG 
GCCCTGGTGG CCTTGGGAGA ACACTCGGAA CTACGAAAGA GCCTTTTGCC ACCTCTCATC 
ATCCACACAG CTGCTACACC GATGTCACTG CCCAAGAGCT CCAGCACAGG TGTGGGGGAA 
GCACTGGGCT CTGGCTCTCG CCGCACCAGT AGCAGTGGGT CCGCTGAGCC TGGAACTGCC 
CATCATGAGA TGAAATCACC GCCAAGTGCC CGAAGCTCCC CGCACAGTCC CTGGAGCGCA 
GCAAGCAGCT GGACCAGCAG GCGCTCCAGC CGGAACAGCC TGGGCCGGGC CCCCAGCCTG 
AAGCGTAGGA GCCCAAGCGG GGAGCGGAGG TCCCTGCTGT CTGGAGAGGG TCAGGAGAGC 
CAGGATGAGG AGGAGAGTTC AGAAGAGGAC CGGGCCAGCC CAGCAGGCAG TGATCATCGC 
CACAGGGGTT CCTTGGAACG TGAGGCCAAG AGTTCCTTTG ACCTGCCTGA CACCCTGCAG 
GTGCCCGGGC TTCATCGAAC AGCCAGCGGT CGGAGCTCTG CCTCTGAACA CCAAGACTGT 
AATGGCAAGT CGGCTTCAGG GCGTTTGGCC CGCACCCTGC GGGCTGATGA CCCCCCACTG 
GATGGGGATG ATGGCGATGA TGAGGGCAAC CTGAGCAAAG GGGAACGCTT ACGAGCCTGG 
GTCCGAGCCC GGCTCCCTGC CTGTTGCCGA GAGCGAGATT CCTGGTCTGC CTATATCTTC 
CCTCCCCAGT CAAGGTTTCG TCTCCTGTGT CACCGGATCA TCACCCACAA GATGTTTGAC 
CATGTGGTCC TCGTCATCAT CTTCCTCAAC TGTATCACCA TCGCTATGGA GCGCCCCAAA 
ATTGACCCCC ACAGCGCTGA ACGCATCTTC CTGACCCTCT CCAACTACAT CTTCACGGCA 
GTCTTCCTGG CTGAAATGAC AGTGAAGGTG GTGGCACTGG GCTGGTGCTT TGGGGAGCAG 
GCCTACCTGC GCAGCAGCTG GAACGTGCTG GACGGCTTGC TGGTGCTCAT CTCTGTCATC 
GACATCCTGG TGTCCATGGT CTCTGACAGC GGCACCAAGA TTCTCGGCAT GCTGAGGGTG 
CTGCGGCTGC TGCGGACCCT ACGTCCACTC AGGGTCATCA GCCGGGCCCA GGGGCTGAAG 
CTGGTGGTAG AGACTCTGAT GTCATCCCTC AAACCCATTG GCAACATTGT GGTCATCTGC 
TGTGCCTTCT TCATCATTTT TGGAATTCTT GGGGTGCAGC TCTTCAAAGG GAAGTTCTTC 
GTGTGTCAGG GTGAGGACAC CAGGAACATC ACTAACAAGT CCGACTGTGC TGAGGCCAGT 
TACCGGTGGG TCCGGCACAA GTACAACTTT GACAACCTGG GCCAGGCTCT GATGTCCCTG 
TTTGTGCTGG CCTCCAAGGA TGGCTGGGTT GACATCATGT ATGATGGACT GGATGCTGTG 
GGAGTGGACC AGCAGCCCAT CATGAACCAC AACCCTTGGA TGCTGCTCTA CTTCATCTCC 
TTCCTCCTCA TCGTGGCCTT CTTCGTCCTG AACATGTTTG TGGGCGTGGT GGTGGAGAAC 
TTCCATAAGT GCAGGCAGCA CCAGGAGGAG GAGGAGGCGC GGCGGCGGGA GGAGAAGCGA 
CTAAAGAGGC TGGAGAAAAA GAGAAGGAAA GCCCAGTGCA AACCCTACTA CTCTGACTAC 
TCGCGCTTCC GGCTCCTCGT CCACCACCTG TGTACCAGCC ACTACCTGGA CCTCTTCATC 
ACTGGTGTCA TCGGGCTGAA TGTGGTCACG ATGGCCATGG AACATTACCA GCAGCCCCAG 
ATCCTGGACG AGGCTCTGAA GATCTGCAAC TACATCTTTA CCGTCATCTT TGTCTTGGAG 
TCAGTATTCA AACTTGTGGC CTTCGGCTTC CGCCGGTTCT TCCAGGACAG GTGGAACCAG 
CTGGACCTGG CTATTGTGCT TCTGTCCATC ATGGGCATCA CGCTGGAAGA GATTGAGGTC 
AATGCTTCAC TGCCCATCAA CCCCACCATC ATCCGTATCA TGAGGGTGCT CCGCATTGCT 
CGAGTTCTGA AGCTGTTGAA GATGGCTGTG GGCATGCGGG CACTGCTGGA CACGGTGATG 
CAGGCCCTGC CCCAGGTGGG GAACCTGGGA CTTCTCTTCA TGCTATTATT TTTCATCTTT 
GCAGCTCTGG GCGTGGAGCT CTTTGGAGAC CTGGAGTGTG ATGAGACACA CCCTTGTGAG 
GGCTTGGGCC GGCATGCCAC CTTTAGGAAC TTTGGTATGG CCTTTCTGAC CCTCTTCCGA 
GTCTCCACTG GTGACAACTG GAATGGTATT ATGAAGGACA CCCTCCGGGA CTGTGACCAG 
GAGTCCACCT GCTACAACAC CGTCATCTCA CCCATCTACT TCGTGTCCTT CGTGCTGACG 
GCCCAGTTTG TGCTGGTCAA CGTGGTCATA GCCGTGCTGA TGAAGCACCT GGAAGAGAGC 
AACAAAGAGG CCAAGGAGGA GGCGGAGTTG GAGGCGGAGC TGGAGCTAGA GATGAAGACA 
CTCAGCCCGC AGCCCCACTC CCCGCTGGGC AGCCCCTTCC TCTGGCCTGG GGTGGAAGGT 
GTCAATAGCC CTGACAGCCC TAAGCCTGGG GCTCCACACA CCACGGCCCA CATTGGAGCA 
GCCTCTTCAG GCTTCTCCCT TGAGCACCCC ACGATGGTAC CTCACACTGA GGAGGGGCCA 
GTCCCCCTAG GACCAGACCT GCTGACTGTG AGGAAGTCTG GTGTCAGCCG GACACACTCT 
CTGCCCAATG ACAGCTACAT GTGCCGCAAT GGGAGCACTG CCGAGAGATC CCTAGGACAC 
AGGGGCTGGG GGCTCCCCAA AGCCCAGTCA GGCTCCATCT TGTCTGTTCA CTCCCAACCA 
GCAGACACCA GCTGCATCCT ACAGCTTCCC AAAGATGCAC ACTATCTGCT CCAGCCTCAT 
GGGGCTCCCA CCTGGGGCGC CATCCCTAAA CTACCCCCAC CTGGCCGCTC CCCTCTGGCT 
CAGAGGCCTC TCAGGCGCCA GGCAGCAATA AGGACTGACT CCCTGGACGT GCAGGGCCTG 
GGTAGCCGGG AAGACCTGTT GTCAGAGGTG AGTGGGCCCT CCTGCCCTCT GACCCGCTCC 
TCATCCTTCT GGGGCGGGTC GAGCATCCAG GTGCAGCAGC GCTCCGGCAG CCAGAGCAAA 
GTCTCCAAGC ACATCCGCCT GCCAGCCCCT TGCCCAGGCC TGGAACCCAG CTGGGCCAAG 
GACCCTCAAG AGACCAGAAG CAGCTTAGAG CTGGACACGG AGCTGAGCTG GATTTCAGGA 
GACCTCCTGC CCAGCAGTCA GGAAGAACCC CTGTCCCCAC GGGACTTGAA AAAATGCTAC 
AGTGTAGAGG CCCAGAGCTG CCGGCGCAGG CCTGGGTCCT GGCTAGACGA ACAGAGGAGA 
CACTCCATCG CTGTCAGCTG CCTGGACAGC GGCTCCCAGC CCCGCCTATG TCCAAGCCCC 
TCAAGCCTCG GGGGCCAACC TCTTGGGGGC CCTGGGAGCC GGCCTAAGAA AAAACTCAGC 
CCACCCAGTA TCTCTATAGA CCCCCCGGAG AGCCAGGGCC CTCGGCCCCC ATGCAGTCCT 
GGCGTCTGCC TCAGGAGGAG GGCGCCGGCC AGTGACTCGA AGGATCCCTC GGCCTCCAGC 
CCCCTTGACA GCACGGCTGC CTCACCCTCC CCAAAGAAAG ATGCGCTGAG TCTCTCTGGT 
TTGTCTTCTG ACCCAACAGA CCTGGATCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532087.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532150.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X30, mRNA.

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CloneID	OMu45482
Clone ID Related Accession (Same CDS sequence)	XM_006532087.3 , XM_006532087.4
Accession Version	XM_006532087.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532087.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGATGGT ACCTCACACT 
GAGGAGGGGC CAGTCCCCCT AGGACCAGAC CTGCTGACTG TGAGGAAGTC TGGTGTCAGC 
CGGACACACT CTCTGCCCAA TGACAGCTAC ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA 
TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT 
CACTCCCAAC CAGCAGACAC CAGCTGCATC CTACAGCTTC CCAAAGATGC ACACTATCTG 
CTCCAGCCTC ATGGGGCTCC CACCTGGGGC GCCATCCCTA AACTACCCCC ACCTGGCCGC 
TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC CAGGCAGCAA TAAGGACTGA CTCCCTGGAC 
GTGCAGGGCC TGGGTAGCCG GGAAGACCTG TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT 
CTGACCCGCT CCTCATCCTT CTGGGGCGGG TCGAGCATCC AGGTGCAGCA GCGCTCCGGC 
AGCCAGAGCA AAGTCTCCAA GCACATCCGC CTGCCAGCCC CTTGCCCAGG CCTGGAACCC 
AGCTGGGCCA AGGACCCTCA AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCAGCAGT CAGGAAGAAC CCCTGTCCCC ACGGGACTTG 
AAAAAATGCT ACAGTGTAGA GGCCCAGAGC TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC 
GAACAGAGGA GACACTCCAT CGCTGTCAGC TGCCTGGACA GCGGCTCCCA GCCCCGCCTA 
TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG 
AAAAAACTCA GCCCACCCAG TATCTCTATA GACCCCCCGG AGAGCCAGGG CCCTCGGCCC 
CCATGCAGTC CTGGCGTCTG CCTCAGGAGG AGGGCGCCGG CCAGTGACTC GAAGGATCCC 
TCGGCCTCCA GCCCCCTTGA CAGCACGGCT GCCTCACCCT CCCCAAAGAA AGATGCGCTG 
AGTCTCTCTG GTTTGTCTTC TGACCCAACA GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532150.1
CDS	773..7537
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532087.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532087.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG CAGATGGCCG AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGATGGT ACCTCACACT 
GAGGAGGGGC CAGTCCCCCT AGGACCAGAC CTGCTGACTG TGAGGAAGTC TGGTGTCAGC 
CGGACACACT CTCTGCCCAA TGACAGCTAC ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA 
TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT 
CACTCCCAAC CAGCAGACAC CAGCTGCATC CTACAGCTTC CCAAAGATGC ACACTATCTG 
CTCCAGCCTC ATGGGGCTCC CACCTGGGGC GCCATCCCTA AACTACCCCC ACCTGGCCGC 
TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC CAGGCAGCAA TAAGGACTGA CTCCCTGGAC 
GTGCAGGGCC TGGGTAGCCG GGAAGACCTG TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT 
CTGACCCGCT CCTCATCCTT CTGGGGCGGG TCGAGCATCC AGGTGCAGCA GCGCTCCGGC 
AGCCAGAGCA AAGTCTCCAA GCACATCCGC CTGCCAGCCC CTTGCCCAGG CCTGGAACCC 
AGCTGGGCCA AGGACCCTCA AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCAGCAGT CAGGAAGAAC CCCTGTCCCC ACGGGACTTG 
AAAAAATGCT ACAGTGTAGA GGCCCAGAGC TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC 
GAACAGAGGA GACACTCCAT CGCTGTCAGC TGCCTGGACA GCGGCTCCCA GCCCCGCCTA 
TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG 
AAAAAACTCA GCCCACCCAG TATCTCTATA GACCCCCCGG AGAGCCAGGG CCCTCGGCCC 
CCATGCAGTC CTGGCGTCTG CCTCAGGAGG AGGGCGCCGG CCAGTGACTC GAAGGATCCC 
TCGGCCTCCA GCCCCCTTGA CAGCACGGCT GCCTCACCCT CCCCAAAGAA AGATGCGCTG 
AGTCTCTCTG GTTTGTCTTC TGACCCAACA GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_006532088.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532151.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X31, mRNA.

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CloneID	OMu45483
Clone ID Related Accession (Same CDS sequence)	XM_006532088.4 , XM_006532088.3
Accession Version	XM_006532088.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532088.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCTGACTA CTCGCGCTTC 
CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ACCTCTTCAT CACTGGTGTC 
ATCGGGCTGA ATGTGGTCAC GATGGCCATG GAACATTACC AGCAGCCCCA GATCCTGGAC 
GAGGCTCTGA AGATCTGCAA CTACATCTTT ACCGTCATCT TTGTCTTGGA GTCAGTATTC 
AAACTTGTGG CCTTCGGCTT CCGCCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCTATTGTGC TTCTGTCCAT CATGGGCATC ACGCTGGAAG AGATTGAGGT CAATGCTTCA 
CTGCCCATCA ACCCCACCAT CATCCGTATC ATGAGGGTGC TCCGCATTGC TCGAGTTCTG 
AAGCTGTTGA AGATGGCTGT GGGCATGCGG GCACTGCTGG ACACGGTGAT GCAGGCCCTG 
CCCCAGGTGG GGAACCTGGG ACTTCTCTTC ATGCTATTAT TTTTCATCTT TGCAGCTCTG 
GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACAC ACCCTTGTGA GGGCTTGGGC 
CGGCATGCCA CCTTTAGGAA CTTTGGTATG GCCTTTCTGA CCCTCTTCCG AGTCTCCACT 
GGTGACAACT GGAATGGTAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC 
TGCTACAACA CCGTCATCTC ACCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG ATGAAGCACC TGGAAGAGAG CAACAAAGAG 
GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG CTGGAGCTAG AGATGAAGAC ACTCAGCCCG 
CAGCCCCACT CCCCGCTGGG CAGCCCCTTC CTCTGGCCTG GGGTGGAAGG TGTCAATAGC 
CCTGACAGCC CTAAGCCTGG GGCTCCACAC ACCACGGCCC ACATTGGAGC AGCCTCTTCA 
GGCTTCTCCC TTGAGCACCC CACGATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532151.1
CDS	773..7516
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_006532088.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532088.3

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGAGCCT GCCATAGCTC CTGCAAAATC TCCAGCCCTT GCTCCAAGGC AGACAGTGGA 
GCCTGTGGGC CGGACAGTTG TCCCTACTGT GCCCGGACAG GAGCAGGGGA GCCAGAGTCC 
GCTGACCATG AAATGCCTGA CTCAGACAGT GAGGCTGTGT ATGAGTTCAC ACAGGACGCT 
CAGCACAGCG ACCTCCGGGA TCCCCACAGA CGGCGACGGC CGAGCCTGGG CCCAGATGCA 
GAGCCTAGTT CTGTGCTGGC CTTCTGGAGG CTGATCTGCG ACACATTCCG GAAGATCGTA 
GATAGCAAAT ACTTTGGCCG GGGAATCATG ATCGCTATCC TGGTCAATAC CCTCAGCATG 
GGCATCGAGT ACCACGAGCA GCCCGAGGAG CTCACCAACG CCCTGGAAAT CAGCAACATC 
GTCTTCACCA GCCTCTTCGC CTTGGAGATG CTGCTGAAGC TGCTCGTCTA CGGTCCCTTC 
GGCTACATTA AGAATCCCTA CAACATCTTT GATGGCGTCA TTGTGGTCAT CAGCGTGTGG 
GAGATTGTGG GCCAGCAGGG AGGTGGCCTG TCGGTGCTGC GGACCTTCCG CCTGATGCGG 
GTGCTGAAGC TAGTTCGCTT CCTGCCGGCA CTGCAGCGGC AGCTCGTGGT GCTCATGAAG 
ACCATGGACA ACGTGGCCAC CTTCTGCATG CTGCTCATGC TCTTCATCTT CATCTTCAGC 
ATCCTGGGCA TGCACCTTTT TGGTTGCAAG TTCGCATCTG AACGGGATGG GGACACGTTG 
CCAGACCGGA AGAATTTTGA CTCCCTGCTC TGGGCCATTG TCACTGTCTT TCAGATTCTG 
ACTCAGGAAG ACTGGAATAA AGTCCTTTAC AACGGCATGG CCTCCACGTC ATCTTGGGCT 
GCTCTTTACT TCATCGCCCT CATGACTTTT GGCAACTACG TGCTCTTTAA CCTGCTTGTC 
GCCATTCTCG TGGAGGGTTT CCAGGCAGAG GGAGATGCCA CCAAGTCTGA GTCAGAGCCT 
GATTTCTTTT CGCCCAGTGT GGATGGTGAT GGGGACAGGA AGAAGCGCTT GGCCCTGGTG 
GCCTTGGGAG AACACTCGGA ACTACGAAAG AGCCTTTTGC CACCTCTCAT CATCCACACA 
GCTGCTACAC CGATGTCACT GCCCAAGAGC TCCAGCACAG GTGTGGGGGA AGCACTGGGC 
TCTGGCTCTC GCCGCACCAG TAGCAGTGGG TCCGCTGAGC CTGGAACTGC CCATCATGAG 
ATGAAATCAC CGCCAAGTGC CCGAAGCTCC CCGCACAGTC CCTGGAGCGC AGCAAGCAGC 
TGGACCAGCA GGCGCTCCAG CCGGAACAGC CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG 
AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG TCTGGAGAGG GTCAGGAGAG CCAGGATGAG 
GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC CCAGCAGGCA GTGATCATCG CCACAGGGGT 
TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT GACCTGCCTG ACACCCTGCA GGTGCCCGGG 
CTTCATCGAA CAGCCAGCGG TCGGAGCTCT GCCTCTGAAC ACCAAGACTG TAATGGCAAG 
TCGGCTTCAG GGCGTTTGGC CCGCACCCTG CGGGCTGATG ACCCCCCACT GGATGGGGAT 
GATGGCGATG ATGAGGGCAA CCTGAGCAAA GGGGAACGCT TACGAGCCTG GGTCCGAGCC 
CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT TCCTGGTCTG CCTATATCTT CCCTCCCCAG 
TCAAGGTTTC GTCTCCTGTG TCACCGGATC ATCACCCACA AGATGTTTGA CCATGTGGTC 
CTCGTCATCA TCTTCCTCAA CTGTATCACC ATCGCTATGG AGCGCCCCAA AATTGACCCC 
CACAGCGCTG AACGCATCTT CCTGACCCTC TCCAACTACA TCTTCACGGC AGTCTTCCTG 
GCTGAAATGA CAGTGAAGGT GGTGGCACTG GGCTGGTGCT TTGGGGAGCA GGCCTACCTG 
CGCAGCAGCT GGAACGTGCT GGACGGCTTG CTGGTGCTCA TCTCTGTCAT CGACATCCTG 
GTGTCCATGG TCTCTGACAG CGGCACCAAG ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG 
CTGCGGACCC TACGTCCACT CAGGGTCATC AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA 
GAGACTCTGA TGTCATCCCT CAAACCCATT GGCAACATTG TGGTCATCTG CTGTGCCTTC 
TTCATCATTT TTGGAATTCT TGGGGTGCAG CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG 
GGTGAGGACA CCAGGAACAT CACTAACAAG TCCGACTGTG CTGAGGCCAG TTACCGGTGG 
GTCCGGCACA AGTACAACTT TGACAACCTG GGCCAGGCTC TGATGTCCCT GTTTGTGCTG 
GCCTCCAAGG ATGGCTGGGT TGACATCATG TATGATGGAC TGGATGCTGT GGGAGTGGAC 
CAGCAGCCCA TCATGAACCA CAACCCTTGG ATGCTGCTCT ACTTCATCTC CTTCCTCCTC 
ATCGTGGCCT TCTTCGTCCT GAACATGTTT GTGGGCGTGG TGGTGGAGAA CTTCCATAAG 
TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG 
CTGGAGAAAA AGAGAAGGAA AGCCCAGTGC AAACCCTACT ACTCTGACTA CTCGCGCTTC 
CGGCTCCTCG TCCACCACCT GTGTACCAGC CACTACCTGG ACCTCTTCAT CACTGGTGTC 
ATCGGGCTGA ATGTGGTCAC GATGGCCATG GAACATTACC AGCAGCCCCA GATCCTGGAC 
GAGGCTCTGA AGATCTGCAA CTACATCTTT ACCGTCATCT TTGTCTTGGA GTCAGTATTC 
AAACTTGTGG CCTTCGGCTT CCGCCGGTTC TTCCAGGACA GGTGGAACCA GCTGGACCTG 
GCTATTGTGC TTCTGTCCAT CATGGGCATC ACGCTGGAAG AGATTGAGGT CAATGCTTCA 
CTGCCCATCA ACCCCACCAT CATCCGTATC ATGAGGGTGC TCCGCATTGC TCGAGTTCTG 
AAGCTGTTGA AGATGGCTGT GGGCATGCGG GCACTGCTGG ACACGGTGAT GCAGGCCCTG 
CCCCAGGTGG GGAACCTGGG ACTTCTCTTC ATGCTATTAT TTTTCATCTT TGCAGCTCTG 
GGCGTGGAGC TCTTTGGAGA CCTGGAGTGT GATGAGACAC ACCCTTGTGA GGGCTTGGGC 
CGGCATGCCA CCTTTAGGAA CTTTGGTATG GCCTTTCTGA CCCTCTTCCG AGTCTCCACT 
GGTGACAACT GGAATGGTAT TATGAAGGAC ACCCTCCGGG ACTGTGACCA GGAGTCCACC 
TGCTACAACA CCGTCATCTC ACCCATCTAC TTCGTGTCCT TCGTGCTGAC GGCCCAGTTT 
GTGCTGGTCA ACGTGGTCAT AGCCGTGCTG ATGAAGCACC TGGAAGAGAG CAACAAAGAG 
GCCAAGGAGG AGGCGGAGTT GGAGGCGGAG CTGGAGCTAG AGATGAAGAC ACTCAGCCCG 
CAGCCCCACT CCCCGCTGGG CAGCCCCTTC CTCTGGCCTG GGGTGGAAGG TGTCAATAGC 
CCTGACAGCC CTAAGCCTGG GGCTCCACAC ACCACGGCCC ACATTGGAGC AGCCTCTTCA 
GGCTTCTCCC TTGAGCACCC CACGATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248686.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246988.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X1, mRNA.

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CloneID	OMu67268
Clone ID Related Accession (Same CDS sequence)	XM_011248686.3 , XM_011248686.2
Accession Version	XM_011248686.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
GACAGACAGC TGTTTGACAC CATCTCCCTG CTGATCCAGG GCTCCCTGGA GGGGGAGCTG 
AAGCTGATGG ACGAGCTGGC AGGCCCAGGG GGCCAGCCCT CTGCCTTCCC TTCCGCCCCC 
AGCCCGGGCG ACTCCGACCC ACAGATCCCT CTAGCTGAGA TGGAGGCTCT GTCTCTGACG 
TCAGAGATTG TGTCTGAACC GTCCTGCTCT CTAGCTCTGA CGGATGACTC TTTGCCTGAT 
GACACTCACA CACTCTTACT TAGTGCCCTG GAGAGCAATA TGGTACCTCA CACTGAGGAG 
GGGCCAGTCC CCCTAGGACC AGACCTGCTG ACTGTGAGGA AGTCTGGTGT CAGCCGGACA 
CACTCTCTGC CCAATGACAG CTACATGTGC CGCAATGGGA GCACTGCCGA GAGATCCCTA 
GGACACAGGG GCTGGGGGCT CCCCAAAGCC CAGTCAGGCT CCATCTTGTC TGTTCACTCC 
CAACCAGCAG ACACCAGCTG CATCCTACAG CTTCCCAAAG ATGCACACTA TCTGCTCCAG 
CCTCATGGGG CTCCCACCTG GGGCGCCATC CCTAAACTAC CCCCACCTGG CCGCTCCCCT 
CTGGCTCAGA GGCCTCTCAG GCGCCAGGCA GCAATAAGGA CTGACTCCCT GGACGTGCAG 
GGCCTGGGTA GCCGGGAAGA CCTGTTGTCA GAGGTGAGTG GGCCCTCCTG CCCTCTGACC 
CGCTCCTCAT CCTTCTGGGG CGGGTCGAGC ATCCAGGTGC AGCAGCGCTC CGGCAGCCAG 
AGCAAAGTCT CCAAGCACAT CCGCCTGCCA GCCCCTTGCC CAGGCCTGGA ACCCAGCTGG 
GCCAAGGACC CTCAAGAGAC CAGAAGCAGC TTAGAGCTGG ACACGGAGCT GAGCTGGATT 
TCAGGAGACC TCCTGCCCAG CAGTCAGGAA GAACCCCTGT CCCCACGGGA CTTGAAAAAA 
TGCTACAGTG TAGAGGCCCA GAGCTGCCGG CGCAGGCCTG GGTCCTGGCT AGACGAACAG 
AGGAGACACT CCATCGCTGT CAGCTGCCTG GACAGCGGCT CCCAGCCCCG CCTATGTCCA 
AGCCCCTCAA GCCTCGGGGG CCAACCTCTT GGGGGCCCTG GGAGCCGGCC TAAGAAAAAA 
CTCAGCCCAC CCAGTATCTC TATAGACCCC CCGGAGAGCC AGGGCCCTCG GCCCCCATGC 
AGTCCTGGCG TCTGCCTCAG GAGGAGGGCG CCGGCCAGTG ACTCGAAGGA TCCCTCGGCC 
TCCAGCCCCC TTGACAGCAC GGCTGCCTCA CCCTCCCCAA AGAAAGATGC GCTGAGTCTC 
TCTGGTTTGT CTTCTGACCC AACAGACCTG GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246988.1
CDS	773..7951
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248686.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248686.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
GACAGACAGC TGTTTGACAC CATCTCCCTG CTGATCCAGG GCTCCCTGGA GGGGGAGCTG 
AAGCTGATGG ACGAGCTGGC AGGCCCAGGG GGCCAGCCCT CTGCCTTCCC TTCCGCCCCC 
AGCCCGGGCG ACTCCGACCC ACAGATCCCT CTAGCTGAGA TGGAGGCTCT GTCTCTGACG 
TCAGAGATTG TGTCTGAACC GTCCTGCTCT CTAGCTCTGA CGGATGACTC TTTGCCTGAT 
GACACTCACA CACTCTTACT TAGTGCCCTG GAGAGCAATA TGGTACCTCA CACTGAGGAG 
GGGCCAGTCC CCCTAGGACC AGACCTGCTG ACTGTGAGGA AGTCTGGTGT CAGCCGGACA 
CACTCTCTGC CCAATGACAG CTACATGTGC CGCAATGGGA GCACTGCCGA GAGATCCCTA 
GGACACAGGG GCTGGGGGCT CCCCAAAGCC CAGTCAGGCT CCATCTTGTC TGTTCACTCC 
CAACCAGCAG ACACCAGCTG CATCCTACAG CTTCCCAAAG ATGCACACTA TCTGCTCCAG 
CCTCATGGGG CTCCCACCTG GGGCGCCATC CCTAAACTAC CCCCACCTGG CCGCTCCCCT 
CTGGCTCAGA GGCCTCTCAG GCGCCAGGCA GCAATAAGGA CTGACTCCCT GGACGTGCAG 
GGCCTGGGTA GCCGGGAAGA CCTGTTGTCA GAGGTGAGTG GGCCCTCCTG CCCTCTGACC 
CGCTCCTCAT CCTTCTGGGG CGGGTCGAGC ATCCAGGTGC AGCAGCGCTC CGGCAGCCAG 
AGCAAAGTCT CCAAGCACAT CCGCCTGCCA GCCCCTTGCC CAGGCCTGGA ACCCAGCTGG 
GCCAAGGACC CTCAAGAGAC CAGAAGCAGC TTAGAGCTGG ACACGGAGCT GAGCTGGATT 
TCAGGAGACC TCCTGCCCAG CAGTCAGGAA GAACCCCTGT CCCCACGGGA CTTGAAAAAA 
TGCTACAGTG TAGAGGCCCA GAGCTGCCGG CGCAGGCCTG GGTCCTGGCT AGACGAACAG 
AGGAGACACT CCATCGCTGT CAGCTGCCTG GACAGCGGCT CCCAGCCCCG CCTATGTCCA 
AGCCCCTCAA GCCTCGGGGG CCAACCTCTT GGGGGCCCTG GGAGCCGGCC TAAGAAAAAA 
CTCAGCCCAC CCAGTATCTC TATAGACCCC CCGGAGAGCC AGGGCCCTCG GCCCCCATGC 
AGTCCTGGCG TCTGCCTCAG GAGGAGGGCG CCGGCCAGTG ACTCGAAGGA TCCCTCGGCC 
TCCAGCCCCC TTGACAGCAC GGCTGCCTCA CCCTCCCCAA AGAAAGATGC GCTGAGTCTC 
TCTGGTTTGT CTTCTGACCC AACAGACCTG GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248687.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246989.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X3, mRNA.

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CloneID	OMu67269
Clone ID Related Accession (Same CDS sequence)	XM_011248687.3 , XM_011248687.2
Accession Version	XM_011248687.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248687.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA ATCTAATGTT GGACGATGTA 
ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG TCAGAAGCCC AGTGCAAACC CTACTACTCT 
GACTACTCGC GCTTCCGGCT CCTCGTCCAC CACCTGTGTA CCAGCCACTA CCTGGACCTC 
TTCATCACTG GTGTCATCGG GCTGAATGTG GTCACGATGG CCATGGAACA TTACCAGCAG 
CCCCAGATCC TGGACGAGGC TCTGAAGATC TGCAACTACA TCTTTACCGT CATCTTTGTC 
TTGGAGTCAG TATTCAAACT TGTGGCCTTC GGCTTCCGCC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCTAT TGTGCTTCTG TCCATCATGG GCATCACGCT GGAAGAGATT 
GAGGTCAATG CTTCACTGCC CATCAACCCC ACCATCATCC GTATCATGAG GGTGCTCCGC 
ATTGCTCGAG TTCTGAAGCT GTTGAAGATG GCTGTGGGCA TGCGGGCACT GCTGGACACG 
GTGATGCAGG CCCTGCCCCA GGTGGGGAAC CTGGGACTTC TCTTCATGCT ATTATTTTTC 
ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACACACCCT 
TGTGAGGGCT TGGGCCGGCA TGCCACCTTT AGGAACTTTG GTATGGCCTT TCTGACCCTC 
TTCCGAGTCT CCACTGGTGA CAACTGGAAT GGTATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACCTGCTA CAACACCGTC ATCTCACCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTCATAGCCG TGCTGATGAA GCACCTGGAA 
GAGAGCAACA AAGAGGCCAA GGAGGAGGCG GAGTTGGAGG CGGAGCTGGA GCTAGAGATG 
AAGACACTCA GCCCGCAGCC CCACTCCCCG CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG 
GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG CCTGGGGCTC CACACACCAC GGCCCACATT 
GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG CACCCCACGG ACAGACAGCT GTTTGACACC 
ATCTCCCTGC TGATCCAGGG CTCCCTGGAG GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT TCCGCCCCCA GCCCGGGCGA CTCCGACCCA 
CAGATCCCTC TAGCTGAGAT GGAGGCTCTG TCTCTGACGT CAGAGATTGT GTCTGAACCG 
TCCTGCTCTC TAGCTCTGAC GGATGACTCT TTGCCTGATG ACACTCACAC ACTCTTACTT 
AGTGCCCTGG AGAGCAATAT GGTACCTCAC ACTGAGGAGG GGCCAGTCCC CCTAGGACCA 
GACCTGCTGA CTGTGAGGAA GTCTGGTGTC AGCCGGACAC ACTCTCTGCC CAATGACAGC 
TACATGTGCC GCAATGGGAG CACTGCCGAG AGATCCCTAG GACACAGGGG CTGGGGGCTC 
CCCAAAGCCC AGTCAGGCTC CATCTTGTCT GTTCACTCCC AACCAGCAGA CACCAGCTGC 
ATCCTACAGC TTCCCAAAGA TGCACACTAT CTGCTCCAGC CTCATGGGGC TCCCACCTGG 
GGCGCCATCC CTAAACTACC CCCACCTGGC CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG 
CGCCAGGCAG CAATAAGGAC TGACTCCCTG GACGTGCAGG GCCTGGGTAG CCGGGAAGAC 
CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC CCTCTGACCC GCTCCTCATC CTTCTGGGGC 
GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC GGCAGCCAGA GCAAAGTCTC CAAGCACATC 
CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA CCCAGCTGGG CCAAGGACCC TCAAGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGCCCAGC 
AGTCAGGAAG AACCCCTGTC CCCACGGGAC TTGAAAAAAT GCTACAGTGT AGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA GACGAACAGA GGAGACACTC CATCGCTGTC 
AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC CTATGTCCAA GCCCCTCAAG CCTCGGGGGC 
CAACCTCTTG GGGGCCCTGG GAGCCGGCCT AAGAAAAAAC TCAGCCCACC CAGTATCTCT 
ATAGACCCCC CGGAGAGCCA GGGCCCTCGG CCCCCATGCA GTCCTGGCGT CTGCCTCAGG 
AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT CCCTCGGCCT CCAGCCCCCT TGACAGCACG 
GCTGCCTCAC CCTCCCCAAA GAAAGATGCG CTGAGTCTCT CTGGTTTGTC TTCTGACCCA 
ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246989.1
CDS	773..7930
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248687.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248687.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA ATCTAATGTT GGACGATGTA 
ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG TCAGAAGCCC AGTGCAAACC CTACTACTCT 
GACTACTCGC GCTTCCGGCT CCTCGTCCAC CACCTGTGTA CCAGCCACTA CCTGGACCTC 
TTCATCACTG GTGTCATCGG GCTGAATGTG GTCACGATGG CCATGGAACA TTACCAGCAG 
CCCCAGATCC TGGACGAGGC TCTGAAGATC TGCAACTACA TCTTTACCGT CATCTTTGTC 
TTGGAGTCAG TATTCAAACT TGTGGCCTTC GGCTTCCGCC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCTAT TGTGCTTCTG TCCATCATGG GCATCACGCT GGAAGAGATT 
GAGGTCAATG CTTCACTGCC CATCAACCCC ACCATCATCC GTATCATGAG GGTGCTCCGC 
ATTGCTCGAG TTCTGAAGCT GTTGAAGATG GCTGTGGGCA TGCGGGCACT GCTGGACACG 
GTGATGCAGG CCCTGCCCCA GGTGGGGAAC CTGGGACTTC TCTTCATGCT ATTATTTTTC 
ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACACACCCT 
TGTGAGGGCT TGGGCCGGCA TGCCACCTTT AGGAACTTTG GTATGGCCTT TCTGACCCTC 
TTCCGAGTCT CCACTGGTGA CAACTGGAAT GGTATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACCTGCTA CAACACCGTC ATCTCACCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTCATAGCCG TGCTGATGAA GCACCTGGAA 
GAGAGCAACA AAGAGGCCAA GGAGGAGGCG GAGTTGGAGG CGGAGCTGGA GCTAGAGATG 
AAGACACTCA GCCCGCAGCC CCACTCCCCG CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG 
GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG CCTGGGGCTC CACACACCAC GGCCCACATT 
GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG CACCCCACGG ACAGACAGCT GTTTGACACC 
ATCTCCCTGC TGATCCAGGG CTCCCTGGAG GGGGAGCTGA AGCTGATGGA CGAGCTGGCA 
GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT TCCGCCCCCA GCCCGGGCGA CTCCGACCCA 
CAGATCCCTC TAGCTGAGAT GGAGGCTCTG TCTCTGACGT CAGAGATTGT GTCTGAACCG 
TCCTGCTCTC TAGCTCTGAC GGATGACTCT TTGCCTGATG ACACTCACAC ACTCTTACTT 
AGTGCCCTGG AGAGCAATAT GGTACCTCAC ACTGAGGAGG GGCCAGTCCC CCTAGGACCA 
GACCTGCTGA CTGTGAGGAA GTCTGGTGTC AGCCGGACAC ACTCTCTGCC CAATGACAGC 
TACATGTGCC GCAATGGGAG CACTGCCGAG AGATCCCTAG GACACAGGGG CTGGGGGCTC 
CCCAAAGCCC AGTCAGGCTC CATCTTGTCT GTTCACTCCC AACCAGCAGA CACCAGCTGC 
ATCCTACAGC TTCCCAAAGA TGCACACTAT CTGCTCCAGC CTCATGGGGC TCCCACCTGG 
GGCGCCATCC CTAAACTACC CCCACCTGGC CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG 
CGCCAGGCAG CAATAAGGAC TGACTCCCTG GACGTGCAGG GCCTGGGTAG CCGGGAAGAC 
CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC CCTCTGACCC GCTCCTCATC CTTCTGGGGC 
GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC GGCAGCCAGA GCAAAGTCTC CAAGCACATC 
CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA CCCAGCTGGG CCAAGGACCC TCAAGAGACC 
AGAAGCAGCT TAGAGCTGGA CACGGAGCTG AGCTGGATTT CAGGAGACCT CCTGCCCAGC 
AGTCAGGAAG AACCCCTGTC CCCACGGGAC TTGAAAAAAT GCTACAGTGT AGAGGCCCAG 
AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA GACGAACAGA GGAGACACTC CATCGCTGTC 
AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC CTATGTCCAA GCCCCTCAAG CCTCGGGGGC 
CAACCTCTTG GGGGCCCTGG GAGCCGGCCT AAGAAAAAAC TCAGCCCACC CAGTATCTCT 
ATAGACCCCC CGGAGAGCCA GGGCCCTCGG CCCCCATGCA GTCCTGGCGT CTGCCTCAGG 
AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT CCCTCGGCCT CCAGCCCCCT TGACAGCACG 
GCTGCCTCAC CCTCCCCAAA GAAAGATGCG CTGAGTCTCT CTGGTTTGTC TTCTGACCCA 
ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248688.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246990.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X5, mRNA.

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CloneID	OMu67270
Clone ID Related Accession (Same CDS sequence)	XM_011248688.2 , XM_011248688.3
Accession Version	XM_011248688.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
TTCTCTGAGA CCAAGCAACG GGAGAGTCAG CTGATGCGGG AGCAGCGTGT ACGATTCCTG 
TCCAATGCTA GCACCCTGGC AAGCTTCTCT GAGCCAGGCA GCTGCTATGA GGAGCTTCTC 
AAGTACCTGG TGTACATCCT CCGCAAAGCA GCCCGCAGGC TGGCCCAGGT CTCTAGGGCT 
GTAGGCGTGC GGGCTGGGTT GCTCAGCAGC CCAGTGGCCC GTGGTGGGCA GGAGCCCCAG 
CCCAGTGGCA GCTGCTCTCG TTCACACCGT CGTCTGTCTG TCCACCACCT GGTCCACCAC 
CATCACCACC ACCATCACCA CTACCACCTG GGTAATGGGA CGCTCAGAGT TCCCCGGGCC 
AGCCCAGAGA TCCAGGACAG GGATGCCAAT GGGTCCCGCT GGCTCATGCT GCCACCACCC 
TCTACCCCCA CTCCCTCTGG GGGCCCTCCG AGGGGTGCGG AGTCTGTACA CAGCTTCTAC 
CATGCTGACT GCCACTTGGA GCCAGTCCGT TGCCAGGCGC CCCCTCCCAG GTCCCCATCG 
GAGGCATCTG GCAGGACTGT GGGTAGTGGG AAGGTATACC CCACTGTGCA TACCAGCCCT 
CCACCAGAGA TGCTGAAGGA TAAGGCACTA GTGGAGGTGG CCCCCAGCCC TGGGCCCCCC 
ACCCTCACCA GCTTCAACAT CCCACCTGGG CCCTTCAGCT CCATGCACAA GCTCCTGGAG 
ACACAGAGTA CGGGTGAGAG CGTGGGAGCC TGCCATAGCT CCTGCAAAAT CTCCAGCCCT 
TGCTCCAAGG CAGACAGTGG AGCCTGTGGG CCGGACAGTT GTCCCTACTG TGCCCGGACA 
GGAGCAGGGG AGCCAGAGTC CGCTGACCAT GAAATGCCTG ACTCAGACAG TGAGGCTGTG 
TATGAGTTCA CACAGGACGC TCAGCACAGC GACCTCCGGG ATCCCCACAG ACGGCGACGG 
CCGAGCCTGG GCCCAGATGC AGAGCCTAGT TCTGTGCTGG CCTTCTGGAG GCTGATCTGC 
GACACATTCC GGAAGATCGT AGATAGCAAA TACTTTGGCC GGGGAATCAT GATCGCTATC 
CTGGTCAATA CCCTCAGCAT GGGCATCGAG TACCACGAGC AGCCCGAGGA GCTCACCAAC 
GCCCTGGAAA TCAGCAACAT CGTCTTCACC AGCCTCTTCG CCTTGGAGAT GCTGCTGAAG 
CTGCTCGTCT ACGGTCCCTT CGGCTACATT AAGAATCCCT ACAACATCTT TGATGGCGTC 
ATTGTGGTCA TCAGCGTGTG GGAGATTGTG GGCCAGCAGG GAGGTGGCCT GTCGGTGCTG 
CGGACCTTCC GCCTGATGCG GGTGCTGAAG CTAGTTCGCT TCCTGCCGGC ACTGCAGCGG 
CAGCTCGTGG TGCTCATGAA GACCATGGAC AACGTGGCCA CCTTCTGCAT GCTGCTCATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTTT TTGGTTGCAA GTTCGCATCT 
GAACGGGATG GGGACACGTT GCCAGACCGG AAGAATTTTG ACTCCCTGCT CTGGGCCATT 
GTCACTGTCT TTCAGATTCT GACTCAGGAA GACTGGAATA AAGTCCTTTA CAACGGCATG 
GCCTCCACGT CATCTTGGGC TGCTCTTTAC TTCATCGCCC TCATGACTTT TGGCAACTAC 
GTGCTCTTTA ACCTGCTTGT CGCCATTCTC GTGGAGGGTT TCCAGGCAGA GGAAATCGGC 
AAACGGGAAG ATACGAGTGG ACAGTTAAGC TGTATTCAGC TGCCTGTCAA CTCCCAGGGG 
GGAGATGCCA CCAAGTCTGA GTCAGAGCCT GATTTCTTTT CGCCCAGTGT GGATGGTGAT 
GGGGACAGGA AGAAGCGCTT GGCCCTGGTG GCCTTGGGAG AACACTCGGA ACTACGAAAG 
AGCCTTTTGC CACCTCTCAT CATCCACACA GCTGCTACAC CGATGTCACT GCCCAAGAGC 
TCCAGCACAG GTGTGGGGGA AGCACTGGGC TCTGGCTCTC GCCGCACCAG TAGCAGTGGG 
TCCGCTGAGC CTGGAACTGC CCATCATGAG ATGAAATCAC CGCCAAGTGC CCGAAGCTCC 
CCGCACAGTC CCTGGAGCGC AGCAAGCAGC TGGACCAGCA GGCGCTCCAG CCGGAACAGC 
CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG 
TCTGGAGAGG GTCAGGAGAG CCAGGATGAG GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC 
CCAGCAGGCA GTGATCATCG CCACAGGGGT TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT 
GACCTGCCTG ACACCCTGCA GGTGCCCGGG CTTCATCGAA CAGCCAGCGG TCGGAGCTCT 
GCCTCTGAAC ACCAAGACTG TAATGGCAAG TCGGCTTCAG GGCGTTTGGC CCGCACCCTG 
CGGGCTGATG ACCCCCCACT GGATGGGGAT GATGGCGATG ATGAGGGCAA CCTGAGCAAA 
GGGGAACGCT TACGAGCCTG GGTCCGAGCC CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT 
TCCTGGTCTG CCTATATCTT CCCTCCCCAG TCAAGGTTTC GTCTCCTGTG TCACCGGATC 
ATCACCCACA AGATGTTTGA CCATGTGGTC CTCGTCATCA TCTTCCTCAA CTGTATCACC 
ATCGCTATGG AGCGCCCCAA AATTGACCCC CACAGCGCTG AACGCATCTT CCTGACCCTC 
TCCAACTACA TCTTCACGGC AGTCTTCCTG GCTGAAATGA CAGTGAAGGT GGTGGCACTG 
GGCTGGTGCT TTGGGGAGCA GGCCTACCTG CGCAGCAGCT GGAACGTGCT GGACGGCTTG 
CTGGTGCTCA TCTCTGTCAT CGACATCCTG GTGTCCATGG TCTCTGACAG CGGCACCAAG 
ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG CTGCGGACCC TACGTCCACT CAGGGTCATC 
AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA GAGACTCTGA TGTCATCCCT CAAACCCATT 
GGCAACATTG TGGTCATCTG CTGTGCCTTC TTCATCATTT TTGGAATTCT TGGGGTGCAG 
CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG GGTGAGGACA CCAGGAACAT CACTAACAAG 
TCCGACTGTG CTGAGGCCAG TTACCGGTGG GTCCGGCACA AGTACAACTT TGACAACCTG 
GGCCAGGCTC TGATGTCCCT GTTTGTGCTG GCCTCCAAGG ATGGCTGGGT TGACATCATG 
TATGATGGAC TGGATGCTGT GGGAGTGGAC CAGCAGCCCA TCATGAACCA CAACCCTTGG 
ATGCTGCTCT ACTTCATCTC CTTCCTCCTC ATCGTGGCCT TCTTCGTCCT GAACATGTTT 
GTGGGCGTGG TGGTGGAGAA CTTCCATAAG TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG 
CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG 
CAGATGGCCG ATCTAATGTT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG 
TCAGAAGCCC AGTGCAAACC CTACTACTCT GACTACTCGC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGACCTC TTCATCACTG GTGTCATCGG GCTGAATGTG 
GTCACGATGG CCATGGAACA TTACCAGCAG CCCCAGATCC TGGACGAGGC TCTGAAGATC 
TGCAACTACA TCTTTACCGT CATCTTTGTC TTGGAGTCAG TATTCAAACT TGTGGCCTTC 
GGCTTCCGCC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCTAT TGTGCTTCTG 
TCCATCATGG GCATCACGCT GGAAGAGATT GAGGTCAATG CTTCACTGCC CATCAACCCC 
ACCATCATCC GTATCATGAG GGTGCTCCGC ATTGCTCGAG TTCTGAAGCT GTTGAAGATG 
GCTGTGGGCA TGCGGGCACT GCTGGACACG GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
CTGGGACTTC TCTTCATGCT ATTATTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGACCTGG AGTGTGATGA GACACACCCT TGTGAGGGCT TGGGCCGGCA TGCCACCTTT 
AGGAACTTTG GTATGGCCTT TCTGACCCTC TTCCGAGTCT CCACTGGTGA CAACTGGAAT 
GGTATTATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACCGTC 
ATCTCACCCA TCTACTTCGT GTCCTTCGTG CTGACGGCCC AGTTTGTGCT GGTCAACGTG 
GTCATAGCCG TGCTGATGAA GCACCTGGAA GAGAGCAACA AAGAGGCCAA GGAGGAGGCG 
GAGTTGGAGG CGGAGCTGGA GCTAGAGATG AAGACACTCA GCCCGCAGCC CCACTCCCCG 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG 
CCTGGGGCTC CACACACCAC GGCCCACATT GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG 
CACCCCACGG ACAGACAGCT GTTTGACACC ATCTCCCTGC TGATCCAGGG CTCCCTGGAG 
GGGGAGCTGA AGCTGATGGA CGAGCTGGCA GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT 
TCCGCCCCCA GCCCGGGCGA CTCCGACCCA CAGATCCCTC TAGCTGAGAT GGAGGCTCTG 
TCTCTGACGT CAGAGATTGT GTCTGAACCG TCCTGCTCTC TAGCTCTGAC GGATGACTCT 
TTGCCTGATG ACACTCACAC ACTCTTACTT AGTGCCCTGG AGAGCAATAT GGTACCTCAC 
ACTGAGGAGG GGCCAGTCCC CCTAGGACCA GACCTGCTGA CTGTGAGGAA GTCTGGTGTC 
AGCCGGACAC ACTCTCTGCC CAATGACAGC TACATGTGCC GCAATGGGAG CACTGCCGAG 
AGATCCCTAG GACACAGGGG CTGGGGGCTC CCCAAAGCCC AGTCAGGCTC CATCTTGTCT 
GTTCACTCCC AACCAGCAGA CACCAGCTGC ATCCTACAGC TTCCCAAAGA TGCACACTAT 
CTGCTCCAGC CTCATGGGGC TCCCACCTGG GGCGCCATCC CTAAACTACC CCCACCTGGC 
CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG CGCCAGGCAG CAATAAGGAC TGACTCCCTG 
GACGTGCAGG GCCTGGGTAG CCGGGAAGAC CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC 
CCTCTGACCC GCTCCTCATC CTTCTGGGGC GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC 
GGCAGCCAGA GCAAAGTCTC CAAGCACATC CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA 
CCCAGCTGGG CCAAGGACCC TCAAGAGACC AGAAGCAGCT TAGAGCTGGA CACGGAGCTG 
AGCTGGATTT CAGGAGACCT CCTGCCCAGC AGTCAGGAAG AACCCCTGTC CCCACGGGAC 
TTGAAAAAAT GCTACAGTGT AGAGGCCCAG AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA 
GACGAACAGA GGAGACACTC CATCGCTGTC AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC 
CTATGTCCAA GCCCCTCAAG CCTCGGGGGC CAACCTCTTG GGGGCCCTGG GAGCCGGCCT 
AAGAAAAAAC TCAGCCCACC CAGTATCTCT ATAGACCCCC CGGAGAGCCA GGGCCCTCGG 
CCCCCATGCA GTCCTGGCGT CTGCCTCAGG AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT 
CCCTCGGCCT CCAGCCCCCT TGACAGCACG GCTGCCTCAC CCTCCCCAAA GAAAGATGCG 
CTGAGTCTCT CTGGTTTGTC TTCTGACCCA ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246990.1
CDS	773..7900
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248688.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248688.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
TTCTCTGAGA CCAAGCAACG GGAGAGTCAG CTGATGCGGG AGCAGCGTGT ACGATTCCTG 
TCCAATGCTA GCACCCTGGC AAGCTTCTCT GAGCCAGGCA GCTGCTATGA GGAGCTTCTC 
AAGTACCTGG TGTACATCCT CCGCAAAGCA GCCCGCAGGC TGGCCCAGGT CTCTAGGGCT 
GTAGGCGTGC GGGCTGGGTT GCTCAGCAGC CCAGTGGCCC GTGGTGGGCA GGAGCCCCAG 
CCCAGTGGCA GCTGCTCTCG TTCACACCGT CGTCTGTCTG TCCACCACCT GGTCCACCAC 
CATCACCACC ACCATCACCA CTACCACCTG GGTAATGGGA CGCTCAGAGT TCCCCGGGCC 
AGCCCAGAGA TCCAGGACAG GGATGCCAAT GGGTCCCGCT GGCTCATGCT GCCACCACCC 
TCTACCCCCA CTCCCTCTGG GGGCCCTCCG AGGGGTGCGG AGTCTGTACA CAGCTTCTAC 
CATGCTGACT GCCACTTGGA GCCAGTCCGT TGCCAGGCGC CCCCTCCCAG GTCCCCATCG 
GAGGCATCTG GCAGGACTGT GGGTAGTGGG AAGGTATACC CCACTGTGCA TACCAGCCCT 
CCACCAGAGA TGCTGAAGGA TAAGGCACTA GTGGAGGTGG CCCCCAGCCC TGGGCCCCCC 
ACCCTCACCA GCTTCAACAT CCCACCTGGG CCCTTCAGCT CCATGCACAA GCTCCTGGAG 
ACACAGAGTA CGGGTGAGAG CGTGGGAGCC TGCCATAGCT CCTGCAAAAT CTCCAGCCCT 
TGCTCCAAGG CAGACAGTGG AGCCTGTGGG CCGGACAGTT GTCCCTACTG TGCCCGGACA 
GGAGCAGGGG AGCCAGAGTC CGCTGACCAT GAAATGCCTG ACTCAGACAG TGAGGCTGTG 
TATGAGTTCA CACAGGACGC TCAGCACAGC GACCTCCGGG ATCCCCACAG ACGGCGACGG 
CCGAGCCTGG GCCCAGATGC AGAGCCTAGT TCTGTGCTGG CCTTCTGGAG GCTGATCTGC 
GACACATTCC GGAAGATCGT AGATAGCAAA TACTTTGGCC GGGGAATCAT GATCGCTATC 
CTGGTCAATA CCCTCAGCAT GGGCATCGAG TACCACGAGC AGCCCGAGGA GCTCACCAAC 
GCCCTGGAAA TCAGCAACAT CGTCTTCACC AGCCTCTTCG CCTTGGAGAT GCTGCTGAAG 
CTGCTCGTCT ACGGTCCCTT CGGCTACATT AAGAATCCCT ACAACATCTT TGATGGCGTC 
ATTGTGGTCA TCAGCGTGTG GGAGATTGTG GGCCAGCAGG GAGGTGGCCT GTCGGTGCTG 
CGGACCTTCC GCCTGATGCG GGTGCTGAAG CTAGTTCGCT TCCTGCCGGC ACTGCAGCGG 
CAGCTCGTGG TGCTCATGAA GACCATGGAC AACGTGGCCA CCTTCTGCAT GCTGCTCATG 
CTCTTCATCT TCATCTTCAG CATCCTGGGC ATGCACCTTT TTGGTTGCAA GTTCGCATCT 
GAACGGGATG GGGACACGTT GCCAGACCGG AAGAATTTTG ACTCCCTGCT CTGGGCCATT 
GTCACTGTCT TTCAGATTCT GACTCAGGAA GACTGGAATA AAGTCCTTTA CAACGGCATG 
GCCTCCACGT CATCTTGGGC TGCTCTTTAC TTCATCGCCC TCATGACTTT TGGCAACTAC 
GTGCTCTTTA ACCTGCTTGT CGCCATTCTC GTGGAGGGTT TCCAGGCAGA GGAAATCGGC 
AAACGGGAAG ATACGAGTGG ACAGTTAAGC TGTATTCAGC TGCCTGTCAA CTCCCAGGGG 
GGAGATGCCA CCAAGTCTGA GTCAGAGCCT GATTTCTTTT CGCCCAGTGT GGATGGTGAT 
GGGGACAGGA AGAAGCGCTT GGCCCTGGTG GCCTTGGGAG AACACTCGGA ACTACGAAAG 
AGCCTTTTGC CACCTCTCAT CATCCACACA GCTGCTACAC CGATGTCACT GCCCAAGAGC 
TCCAGCACAG GTGTGGGGGA AGCACTGGGC TCTGGCTCTC GCCGCACCAG TAGCAGTGGG 
TCCGCTGAGC CTGGAACTGC CCATCATGAG ATGAAATCAC CGCCAAGTGC CCGAAGCTCC 
CCGCACAGTC CCTGGAGCGC AGCAAGCAGC TGGACCAGCA GGCGCTCCAG CCGGAACAGC 
CTGGGCCGGG CCCCCAGCCT GAAGCGTAGG AGCCCAAGCG GGGAGCGGAG GTCCCTGCTG 
TCTGGAGAGG GTCAGGAGAG CCAGGATGAG GAGGAGAGTT CAGAAGAGGA CCGGGCCAGC 
CCAGCAGGCA GTGATCATCG CCACAGGGGT TCCTTGGAAC GTGAGGCCAA GAGTTCCTTT 
GACCTGCCTG ACACCCTGCA GGTGCCCGGG CTTCATCGAA CAGCCAGCGG TCGGAGCTCT 
GCCTCTGAAC ACCAAGACTG TAATGGCAAG TCGGCTTCAG GGCGTTTGGC CCGCACCCTG 
CGGGCTGATG ACCCCCCACT GGATGGGGAT GATGGCGATG ATGAGGGCAA CCTGAGCAAA 
GGGGAACGCT TACGAGCCTG GGTCCGAGCC CGGCTCCCTG CCTGTTGCCG AGAGCGAGAT 
TCCTGGTCTG CCTATATCTT CCCTCCCCAG TCAAGGTTTC GTCTCCTGTG TCACCGGATC 
ATCACCCACA AGATGTTTGA CCATGTGGTC CTCGTCATCA TCTTCCTCAA CTGTATCACC 
ATCGCTATGG AGCGCCCCAA AATTGACCCC CACAGCGCTG AACGCATCTT CCTGACCCTC 
TCCAACTACA TCTTCACGGC AGTCTTCCTG GCTGAAATGA CAGTGAAGGT GGTGGCACTG 
GGCTGGTGCT TTGGGGAGCA GGCCTACCTG CGCAGCAGCT GGAACGTGCT GGACGGCTTG 
CTGGTGCTCA TCTCTGTCAT CGACATCCTG GTGTCCATGG TCTCTGACAG CGGCACCAAG 
ATTCTCGGCA TGCTGAGGGT GCTGCGGCTG CTGCGGACCC TACGTCCACT CAGGGTCATC 
AGCCGGGCCC AGGGGCTGAA GCTGGTGGTA GAGACTCTGA TGTCATCCCT CAAACCCATT 
GGCAACATTG TGGTCATCTG CTGTGCCTTC TTCATCATTT TTGGAATTCT TGGGGTGCAG 
CTCTTCAAAG GGAAGTTCTT CGTGTGTCAG GGTGAGGACA CCAGGAACAT CACTAACAAG 
TCCGACTGTG CTGAGGCCAG TTACCGGTGG GTCCGGCACA AGTACAACTT TGACAACCTG 
GGCCAGGCTC TGATGTCCCT GTTTGTGCTG GCCTCCAAGG ATGGCTGGGT TGACATCATG 
TATGATGGAC TGGATGCTGT GGGAGTGGAC CAGCAGCCCA TCATGAACCA CAACCCTTGG 
ATGCTGCTCT ACTTCATCTC CTTCCTCCTC ATCGTGGCCT TCTTCGTCCT GAACATGTTT 
GTGGGCGTGG TGGTGGAGAA CTTCCATAAG TGCAGGCAGC ACCAGGAGGA GGAGGAGGCG 
CGGCGGCGGG AGGAGAAGCG ACTAAAGAGG CTGGAGAAAA AGAGAAGGAG TAAGGAGAAG 
CAGATGGCCG ATCTAATGTT GGACGATGTA ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG 
TCAGAAGCCC AGTGCAAACC CTACTACTCT GACTACTCGC GCTTCCGGCT CCTCGTCCAC 
CACCTGTGTA CCAGCCACTA CCTGGACCTC TTCATCACTG GTGTCATCGG GCTGAATGTG 
GTCACGATGG CCATGGAACA TTACCAGCAG CCCCAGATCC TGGACGAGGC TCTGAAGATC 
TGCAACTACA TCTTTACCGT CATCTTTGTC TTGGAGTCAG TATTCAAACT TGTGGCCTTC 
GGCTTCCGCC GGTTCTTCCA GGACAGGTGG AACCAGCTGG ACCTGGCTAT TGTGCTTCTG 
TCCATCATGG GCATCACGCT GGAAGAGATT GAGGTCAATG CTTCACTGCC CATCAACCCC 
ACCATCATCC GTATCATGAG GGTGCTCCGC ATTGCTCGAG TTCTGAAGCT GTTGAAGATG 
GCTGTGGGCA TGCGGGCACT GCTGGACACG GTGATGCAGG CCCTGCCCCA GGTGGGGAAC 
CTGGGACTTC TCTTCATGCT ATTATTTTTC ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT 
GGAGACCTGG AGTGTGATGA GACACACCCT TGTGAGGGCT TGGGCCGGCA TGCCACCTTT 
AGGAACTTTG GTATGGCCTT TCTGACCCTC TTCCGAGTCT CCACTGGTGA CAACTGGAAT 
GGTATTATGA AGGACACCCT CCGGGACTGT GACCAGGAGT CCACCTGCTA CAACACCGTC 
ATCTCACCCA TCTACTTCGT GTCCTTCGTG CTGACGGCCC AGTTTGTGCT GGTCAACGTG 
GTCATAGCCG TGCTGATGAA GCACCTGGAA GAGAGCAACA AAGAGGCCAA GGAGGAGGCG 
GAGTTGGAGG CGGAGCTGGA GCTAGAGATG AAGACACTCA GCCCGCAGCC CCACTCCCCG 
CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG 
CCTGGGGCTC CACACACCAC GGCCCACATT GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG 
CACCCCACGG ACAGACAGCT GTTTGACACC ATCTCCCTGC TGATCCAGGG CTCCCTGGAG 
GGGGAGCTGA AGCTGATGGA CGAGCTGGCA GGCCCAGGGG GCCAGCCCTC TGCCTTCCCT 
TCCGCCCCCA GCCCGGGCGA CTCCGACCCA CAGATCCCTC TAGCTGAGAT GGAGGCTCTG 
TCTCTGACGT CAGAGATTGT GTCTGAACCG TCCTGCTCTC TAGCTCTGAC GGATGACTCT 
TTGCCTGATG ACACTCACAC ACTCTTACTT AGTGCCCTGG AGAGCAATAT GGTACCTCAC 
ACTGAGGAGG GGCCAGTCCC CCTAGGACCA GACCTGCTGA CTGTGAGGAA GTCTGGTGTC 
AGCCGGACAC ACTCTCTGCC CAATGACAGC TACATGTGCC GCAATGGGAG CACTGCCGAG 
AGATCCCTAG GACACAGGGG CTGGGGGCTC CCCAAAGCCC AGTCAGGCTC CATCTTGTCT 
GTTCACTCCC AACCAGCAGA CACCAGCTGC ATCCTACAGC TTCCCAAAGA TGCACACTAT 
CTGCTCCAGC CTCATGGGGC TCCCACCTGG GGCGCCATCC CTAAACTACC CCCACCTGGC 
CGCTCCCCTC TGGCTCAGAG GCCTCTCAGG CGCCAGGCAG CAATAAGGAC TGACTCCCTG 
GACGTGCAGG GCCTGGGTAG CCGGGAAGAC CTGTTGTCAG AGGTGAGTGG GCCCTCCTGC 
CCTCTGACCC GCTCCTCATC CTTCTGGGGC GGGTCGAGCA TCCAGGTGCA GCAGCGCTCC 
GGCAGCCAGA GCAAAGTCTC CAAGCACATC CGCCTGCCAG CCCCTTGCCC AGGCCTGGAA 
CCCAGCTGGG CCAAGGACCC TCAAGAGACC AGAAGCAGCT TAGAGCTGGA CACGGAGCTG 
AGCTGGATTT CAGGAGACCT CCTGCCCAGC AGTCAGGAAG AACCCCTGTC CCCACGGGAC 
TTGAAAAAAT GCTACAGTGT AGAGGCCCAG AGCTGCCGGC GCAGGCCTGG GTCCTGGCTA 
GACGAACAGA GGAGACACTC CATCGCTGTC AGCTGCCTGG ACAGCGGCTC CCAGCCCCGC 
CTATGTCCAA GCCCCTCAAG CCTCGGGGGC CAACCTCTTG GGGGCCCTGG GAGCCGGCCT 
AAGAAAAAAC TCAGCCCACC CAGTATCTCT ATAGACCCCC CGGAGAGCCA GGGCCCTCGG 
CCCCCATGCA GTCCTGGCGT CTGCCTCAGG AGGAGGGCGC CGGCCAGTGA CTCGAAGGAT 
CCCTCGGCCT CCAGCCCCCT TGACAGCACG GCTGCCTCAC CCTCCCCAAA GAAAGATGCG 
CTGAGTCTCT CTGGTTTGTC TTCTGACCCA ACAGACCTGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248689.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246991.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X6, mRNA.

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CloneID	OMu67271
Clone ID Related Accession (Same CDS sequence)	XM_011248689.2 , XM_011248689.3
Accession Version	XM_011248689.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GAAGCCCAGT GCAAACCCTA CTACTCTGAC TACTCGCGCT TCCGGCTCCT CGTCCACCAC 
CTGTGTACCA GCCACTACCT GGACCTCTTC ATCACTGGTG TCATCGGGCT GAATGTGGTC 
ACGATGGCCA TGGAACATTA CCAGCAGCCC CAGATCCTGG ACGAGGCTCT GAAGATCTGC 
AACTACATCT TTACCGTCAT CTTTGTCTTG GAGTCAGTAT TCAAACTTGT GGCCTTCGGC 
TTCCGCCGGT TCTTCCAGGA CAGGTGGAAC CAGCTGGACC TGGCTATTGT GCTTCTGTCC 
ATCATGGGCA TCACGCTGGA AGAGATTGAG GTCAATGCTT CACTGCCCAT CAACCCCACC 
ATCATCCGTA TCATGAGGGT GCTCCGCATT GCTCGAGTTC TGAAGCTGTT GAAGATGGCT 
GTGGGCATGC GGGCACTGCT GGACACGGTG ATGCAGGCCC TGCCCCAGGT GGGGAACCTG 
GGACTTCTCT TCATGCTATT ATTTTTCATC TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA 
GACCTGGAGT GTGATGAGAC ACACCCTTGT GAGGGCTTGG GCCGGCATGC CACCTTTAGG 
AACTTTGGTA TGGCCTTTCT GACCCTCTTC CGAGTCTCCA CTGGTGACAA CTGGAATGGT 
ATTATGAAGG ACACCCTCCG GGACTGTGAC CAGGAGTCCA CCTGCTACAA CACCGTCATC 
TCACCCATCT ACTTCGTGTC CTTCGTGCTG ACGGCCCAGT TTGTGCTGGT CAACGTGGTC 
ATAGCCGTGC TGATGAAGCA CCTGGAAGAG AGCAACAAAG AGGCCAAGGA GGAGGCGGAG 
TTGGAGGCGG AGCTGGAGCT AGAGATGAAG ACACTCAGCC CGCAGCCCCA CTCCCCGCTG 
GGCAGCCCCT TCCTCTGGCC TGGGGTGGAA GGTGTCAATA GCCCTGACAG CCCTAAGCCT 
GGGGCTCCAC ACACCACGGC CCACATTGGA GCAGCCTCTT CAGGCTTCTC CCTTGAGCAC 
CCCACGGACA GACAGCTGTT TGACACCATC TCCCTGCTGA TCCAGGGCTC CCTGGAGGGG 
GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTTCC 
GCCCCCAGCC CGGGCGACTC CGACCCACAG ATCCCTCTAG CTGAGATGGA GGCTCTGTCT 
CTGACGTCAG AGATTGTGTC TGAACCGTCC TGCTCTCTAG CTCTGACGGA TGACTCTTTG 
CCTGATGACA CTCACACACT CTTACTTAGT GCCCTGGAGA GCAATATGGT ACCTCACACT 
GAGGAGGGGC CAGTCCCCCT AGGACCAGAC CTGCTGACTG TGAGGAAGTC TGGTGTCAGC 
CGGACACACT CTCTGCCCAA TGACAGCTAC ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA 
TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT 
CACTCCCAAC CAGCAGACAC CAGCTGCATC CTACAGCTTC CCAAAGATGC ACACTATCTG 
CTCCAGCCTC ATGGGGCTCC CACCTGGGGC GCCATCCCTA AACTACCCCC ACCTGGCCGC 
TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC CAGGCAGCAA TAAGGACTGA CTCCCTGGAC 
GTGCAGGGCC TGGGTAGCCG GGAAGACCTG TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT 
CTGACCCGCT CCTCATCCTT CTGGGGCGGG TCGAGCATCC AGGTGCAGCA GCGCTCCGGC 
AGCCAGAGCA AAGTCTCCAA GCACATCCGC CTGCCAGCCC CTTGCCCAGG CCTGGAACCC 
AGCTGGGCCA AGGACCCTCA AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCAGCAGT CAGGAAGAAC CCCTGTCCCC ACGGGACTTG 
AAAAAATGCT ACAGTGTAGA GGCCCAGAGC TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC 
GAACAGAGGA GACACTCCAT CGCTGTCAGC TGCCTGGACA GCGGCTCCCA GCCCCGCCTA 
TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG 
AAAAAACTCA GCCCACCCAG TATCTCTATA GACCCCCCGG AGAGCCAGGG CCCTCGGCCC 
CCATGCAGTC CTGGCGTCTG CCTCAGGAGG AGGGCGCCGG CCAGTGACTC GAAGGATCCC 
TCGGCCTCCA GCCCCCTTGA CAGCACGGCT GCCTCACCCT CCCCAAAGAA AGATGCGCTG 
AGTCTCTCTG GTTTGTCTTC TGACCCAACA GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246991.1
CDS	773..7897
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248689.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248689.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GAAGCCCAGT GCAAACCCTA CTACTCTGAC TACTCGCGCT TCCGGCTCCT CGTCCACCAC 
CTGTGTACCA GCCACTACCT GGACCTCTTC ATCACTGGTG TCATCGGGCT GAATGTGGTC 
ACGATGGCCA TGGAACATTA CCAGCAGCCC CAGATCCTGG ACGAGGCTCT GAAGATCTGC 
AACTACATCT TTACCGTCAT CTTTGTCTTG GAGTCAGTAT TCAAACTTGT GGCCTTCGGC 
TTCCGCCGGT TCTTCCAGGA CAGGTGGAAC CAGCTGGACC TGGCTATTGT GCTTCTGTCC 
ATCATGGGCA TCACGCTGGA AGAGATTGAG GTCAATGCTT CACTGCCCAT CAACCCCACC 
ATCATCCGTA TCATGAGGGT GCTCCGCATT GCTCGAGTTC TGAAGCTGTT GAAGATGGCT 
GTGGGCATGC GGGCACTGCT GGACACGGTG ATGCAGGCCC TGCCCCAGGT GGGGAACCTG 
GGACTTCTCT TCATGCTATT ATTTTTCATC TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA 
GACCTGGAGT GTGATGAGAC ACACCCTTGT GAGGGCTTGG GCCGGCATGC CACCTTTAGG 
AACTTTGGTA TGGCCTTTCT GACCCTCTTC CGAGTCTCCA CTGGTGACAA CTGGAATGGT 
ATTATGAAGG ACACCCTCCG GGACTGTGAC CAGGAGTCCA CCTGCTACAA CACCGTCATC 
TCACCCATCT ACTTCGTGTC CTTCGTGCTG ACGGCCCAGT TTGTGCTGGT CAACGTGGTC 
ATAGCCGTGC TGATGAAGCA CCTGGAAGAG AGCAACAAAG AGGCCAAGGA GGAGGCGGAG 
TTGGAGGCGG AGCTGGAGCT AGAGATGAAG ACACTCAGCC CGCAGCCCCA CTCCCCGCTG 
GGCAGCCCCT TCCTCTGGCC TGGGGTGGAA GGTGTCAATA GCCCTGACAG CCCTAAGCCT 
GGGGCTCCAC ACACCACGGC CCACATTGGA GCAGCCTCTT CAGGCTTCTC CCTTGAGCAC 
CCCACGGACA GACAGCTGTT TGACACCATC TCCCTGCTGA TCCAGGGCTC CCTGGAGGGG 
GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTTCC 
GCCCCCAGCC CGGGCGACTC CGACCCACAG ATCCCTCTAG CTGAGATGGA GGCTCTGTCT 
CTGACGTCAG AGATTGTGTC TGAACCGTCC TGCTCTCTAG CTCTGACGGA TGACTCTTTG 
CCTGATGACA CTCACACACT CTTACTTAGT GCCCTGGAGA GCAATATGGT ACCTCACACT 
GAGGAGGGGC CAGTCCCCCT AGGACCAGAC CTGCTGACTG TGAGGAAGTC TGGTGTCAGC 
CGGACACACT CTCTGCCCAA TGACAGCTAC ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA 
TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT 
CACTCCCAAC CAGCAGACAC CAGCTGCATC CTACAGCTTC CCAAAGATGC ACACTATCTG 
CTCCAGCCTC ATGGGGCTCC CACCTGGGGC GCCATCCCTA AACTACCCCC ACCTGGCCGC 
TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC CAGGCAGCAA TAAGGACTGA CTCCCTGGAC 
GTGCAGGGCC TGGGTAGCCG GGAAGACCTG TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT 
CTGACCCGCT CCTCATCCTT CTGGGGCGGG TCGAGCATCC AGGTGCAGCA GCGCTCCGGC 
AGCCAGAGCA AAGTCTCCAA GCACATCCGC CTGCCAGCCC CTTGCCCAGG CCTGGAACCC 
AGCTGGGCCA AGGACCCTCA AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCAGCAGT CAGGAAGAAC CCCTGTCCCC ACGGGACTTG 
AAAAAATGCT ACAGTGTAGA GGCCCAGAGC TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC 
GAACAGAGGA GACACTCCAT CGCTGTCAGC TGCCTGGACA GCGGCTCCCA GCCCCGCCTA 
TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG 
AAAAAACTCA GCCCACCCAG TATCTCTATA GACCCCCCGG AGAGCCAGGG CCCTCGGCCC 
CCATGCAGTC CTGGCGTCTG CCTCAGGAGG AGGGCGCCGG CCAGTGACTC GAAGGATCCC 
TCGGCCTCCA GCCCCCTTGA CAGCACGGCT GCCTCACCCT CCCCAAAGAA AGATGCGCTG 
AGTCTCTCTG GTTTGTCTTC TGACCCAACA GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248690.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246992.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X8, mRNA.

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CloneID	OMu67272
Clone ID Related Accession (Same CDS sequence)	XM_011248690.2 , XM_011248690.3
Accession Version	XM_011248690.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGGAGATGC CACCAAGTCT 
GAGTCAGAGC CTGATTTCTT TTCGCCCAGT GTGGATGGTG ATGGGGACAG GAAGAAGCGC 
TTGGCCCTGG TGGCCTTGGG AGAACACTCG GAACTACGAA AGAGCCTTTT GCCACCTCTC 
ATCATCCACA CAGCTGCTAC ACCGATGTCA CTGCCCAAGA GCTCCAGCAC AGGTGTGGGG 
GAAGCACTGG GCTCTGGCTC TCGCCGCACC AGTAGCAGTG GGTCCGCTGA GCCTGGAACT 
GCCCATCATG AGATGAAATC ACCGCCAAGT GCCCGAAGCT CCCCGCACAG TCCCTGGAGC 
GCAGCAAGCA GCTGGACCAG CAGGCGCTCC AGCCGGAACA GCCTGGGCCG GGCCCCCAGC 
CTGAAGCGTA GGAGCCCAAG CGGGGAGCGG AGGTCCCTGC TGTCTGGAGA GGGTCAGGAG 
AGCCAGGATG AGGAGGAGAG TTCAGAAGAG GACCGGGCCA GCCCAGCAGG CAGTGATCAT 
CGCCACAGGG GTTCCTTGGA ACGTGAGGCC AAGAGTTCCT TTGACCTGCC TGACACCCTG 
CAGGTGCCCG GGCTTCATCG AACAGCCAGC GGTCGGAGCT CTGCCTCTGA ACACCAAGAC 
TGTAATGGCA AGTCGGCTTC AGGGCGTTTG GCCCGCACCC TGCGGGCTGA TGACCCCCCA 
CTGGATGGGG ATGATGGCGA TGATGAGGGC AACCTGAGCA AAGGGGAACG CTTACGAGCC 
TGGGTCCGAG CCCGGCTCCC TGCCTGTTGC CGAGAGCGAG ATTCCTGGTC TGCCTATATC 
TTCCCTCCCC AGTCAAGGTT TCGTCTCCTG TGTCACCGGA TCATCACCCA CAAGATGTTT 
GACCATGTGG TCCTCGTCAT CATCTTCCTC AACTGTATCA CCATCGCTAT GGAGCGCCCC 
AAAATTGACC CCCACAGCGC TGAACGCATC TTCCTGACCC TCTCCAACTA CATCTTCACG 
GCAGTCTTCC TGGCTGAAAT GACAGTGAAG GTGGTGGCAC TGGGCTGGTG CTTTGGGGAG 
CAGGCCTACC TGCGCAGCAG CTGGAACGTG CTGGACGGCT TGCTGGTGCT CATCTCTGTC 
ATCGACATCC TGGTGTCCAT GGTCTCTGAC AGCGGCACCA AGATTCTCGG CATGCTGAGG 
GTGCTGCGGC TGCTGCGGAC CCTACGTCCA CTCAGGGTCA TCAGCCGGGC CCAGGGGCTG 
AAGCTGGTGG TAGAGACTCT GATGTCATCC CTCAAACCCA TTGGCAACAT TGTGGTCATC 
TGCTGTGCCT TCTTCATCAT TTTTGGAATT CTTGGGGTGC AGCTCTTCAA AGGGAAGTTC 
TTCGTGTGTC AGGGTGAGGA CACCAGGAAC ATCACTAACA AGTCCGACTG TGCTGAGGCC 
AGTTACCGGT GGGTCCGGCA CAAGTACAAC TTTGACAACC TGGGCCAGGC TCTGATGTCC 
CTGTTTGTGC TGGCCTCCAA GGATGGCTGG GTTGACATCA TGTATGATGG ACTGGATGCT 
GTGGGAGTGG ACCAGCAGCC CATCATGAAC CACAACCCTT GGATGCTGCT CTACTTCATC 
TCCTTCCTCC TCATCGTGGC CTTCTTCGTC CTGAACATGT TTGTGGGCGT GGTGGTGGAG 
AACTTCCATA AGTGCAGGCA GCACCAGGAG GAGGAGGAGG CGCGGCGGCG GGAGGAGAAG 
CGACTAAAGA GGCTGGAGAA AAAGAGAAGG AGTAAGGAGA AGCAGATGGC CGATCTAATG 
TTGGACGATG TAATTGCTTC CGGCAGCTCA GCCAGCGCTG CGTCAGAAGC CCAGTGCAAA 
CCCTACTACT CTGACTACTC GCGCTTCCGG CTCCTCGTCC ACCACCTGTG TACCAGCCAC 
TACCTGGACC TCTTCATCAC TGGTGTCATC GGGCTGAATG TGGTCACGAT GGCCATGGAA 
CATTACCAGC AGCCCCAGAT CCTGGACGAG GCTCTGAAGA TCTGCAACTA CATCTTTACC 
GTCATCTTTG TCTTGGAGTC AGTATTCAAA CTTGTGGCCT TCGGCTTCCG CCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCT ATTGTGCTTC TGTCCATCAT GGGCATCACG 
CTGGAAGAGA TTGAGGTCAA TGCTTCACTG CCCATCAACC CCACCATCAT CCGTATCATG 
AGGGTGCTCC GCATTGCTCG AGTTCTGAAG CTGTTGAAGA TGGCTGTGGG CATGCGGGCA 
CTGCTGGACA CGGTGATGCA GGCCCTGCCC CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
CTATTATTTT TCATCTTTGC AGCTCTGGGC GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACACACC CTTGTGAGGG CTTGGGCCGG CATGCCACCT TTAGGAACTT TGGTATGGCC 
TTTCTGACCC TCTTCCGAGT CTCCACTGGT GACAACTGGA ATGGTATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACCTGC TACAACACCG TCATCTCACC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTCATAGC CGTGCTGATG 
AAGCACCTGG AAGAGAGCAA CAAAGAGGCC AAGGAGGAGG CGGAGTTGGA GGCGGAGCTG 
GAGCTAGAGA TGAAGACACT CAGCCCGCAG CCCCACTCCC CGCTGGGCAG CCCCTTCCTC 
TGGCCTGGGG TGGAAGGTGT CAATAGCCCT GACAGCCCTA AGCCTGGGGC TCCACACACC 
ACGGCCCACA TTGGAGCAGC CTCTTCAGGC TTCTCCCTTG AGCACCCCAC GGACAGACAG 
CTGTTTGACA CCATCTCCCT GCTGATCCAG GGCTCCCTGG AGGGGGAGCT GAAGCTGATG 
GACGAGCTGG CAGGCCCAGG GGGCCAGCCC TCTGCCTTCC CTTCCGCCCC CAGCCCGGGC 
GACTCCGACC CACAGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTCTGAC GTCAGAGATT 
GTGTCTGAAC CGTCCTGCTC TCTAGCTCTG ACGGATGACT CTTTGCCTGA TGACACTCAC 
ACACTCTTAC TTAGTGCCCT GGAGAGCAAT ATGGTACCTC ACACTGAGGA GGGGCCAGTC 
CCCCTAGGAC CAGACCTGCT GACTGTGAGG AAGTCTGGTG TCAGCCGGAC ACACTCTCTG 
CCCAATGACA GCTACATGTG CCGCAATGGG AGCACTGCCG AGAGATCCCT AGGACACAGG 
GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC TCCATCTTGT CTGTTCACTC CCAACCAGCA 
GACACCAGCT GCATCCTACA GCTTCCCAAA GATGCACACT ATCTGCTCCA GCCTCATGGG 
GCTCCCACCT GGGGCGCCAT CCCTAAACTA CCCCCACCTG GCCGCTCCCC TCTGGCTCAG 
AGGCCTCTCA GGCGCCAGGC AGCAATAAGG ACTGACTCCC TGGACGTGCA GGGCCTGGGT 
AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA 
TCCTTCTGGG GCGGGTCGAG CATCCAGGTG CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC 
TCCAAGCACA TCCGCCTGCC AGCCCCTTGC CCAGGCCTGG AACCCAGCTG GGCCAAGGAC 
CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGCCCA GCAGTCAGGA AGAACCCCTG TCCCCACGGG ACTTGAAAAA ATGCTACAGT 
GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT GGGTCCTGGC TAGACGAACA GAGGAGACAC 
TCCATCGCTG TCAGCTGCCT GGACAGCGGC TCCCAGCCCC GCCTATGTCC AAGCCCCTCA 
AGCCTCGGGG GCCAACCTCT TGGGGGCCCT GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA 
CCCAGTATCT CTATAGACCC CCCGGAGAGC CAGGGCCCTC GGCCCCCATG CAGTCCTGGC 
GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT GACTCGAAGG ATCCCTCGGC CTCCAGCCCC 
CTTGACAGCA CGGCTGCCTC ACCCTCCCCA AAGAAAGATG CGCTGAGTCT CTCTGGTTTG 
TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246992.1
CDS	773..7882
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248690.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248690.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGGAGATGC CACCAAGTCT 
GAGTCAGAGC CTGATTTCTT TTCGCCCAGT GTGGATGGTG ATGGGGACAG GAAGAAGCGC 
TTGGCCCTGG TGGCCTTGGG AGAACACTCG GAACTACGAA AGAGCCTTTT GCCACCTCTC 
ATCATCCACA CAGCTGCTAC ACCGATGTCA CTGCCCAAGA GCTCCAGCAC AGGTGTGGGG 
GAAGCACTGG GCTCTGGCTC TCGCCGCACC AGTAGCAGTG GGTCCGCTGA GCCTGGAACT 
GCCCATCATG AGATGAAATC ACCGCCAAGT GCCCGAAGCT CCCCGCACAG TCCCTGGAGC 
GCAGCAAGCA GCTGGACCAG CAGGCGCTCC AGCCGGAACA GCCTGGGCCG GGCCCCCAGC 
CTGAAGCGTA GGAGCCCAAG CGGGGAGCGG AGGTCCCTGC TGTCTGGAGA GGGTCAGGAG 
AGCCAGGATG AGGAGGAGAG TTCAGAAGAG GACCGGGCCA GCCCAGCAGG CAGTGATCAT 
CGCCACAGGG GTTCCTTGGA ACGTGAGGCC AAGAGTTCCT TTGACCTGCC TGACACCCTG 
CAGGTGCCCG GGCTTCATCG AACAGCCAGC GGTCGGAGCT CTGCCTCTGA ACACCAAGAC 
TGTAATGGCA AGTCGGCTTC AGGGCGTTTG GCCCGCACCC TGCGGGCTGA TGACCCCCCA 
CTGGATGGGG ATGATGGCGA TGATGAGGGC AACCTGAGCA AAGGGGAACG CTTACGAGCC 
TGGGTCCGAG CCCGGCTCCC TGCCTGTTGC CGAGAGCGAG ATTCCTGGTC TGCCTATATC 
TTCCCTCCCC AGTCAAGGTT TCGTCTCCTG TGTCACCGGA TCATCACCCA CAAGATGTTT 
GACCATGTGG TCCTCGTCAT CATCTTCCTC AACTGTATCA CCATCGCTAT GGAGCGCCCC 
AAAATTGACC CCCACAGCGC TGAACGCATC TTCCTGACCC TCTCCAACTA CATCTTCACG 
GCAGTCTTCC TGGCTGAAAT GACAGTGAAG GTGGTGGCAC TGGGCTGGTG CTTTGGGGAG 
CAGGCCTACC TGCGCAGCAG CTGGAACGTG CTGGACGGCT TGCTGGTGCT CATCTCTGTC 
ATCGACATCC TGGTGTCCAT GGTCTCTGAC AGCGGCACCA AGATTCTCGG CATGCTGAGG 
GTGCTGCGGC TGCTGCGGAC CCTACGTCCA CTCAGGGTCA TCAGCCGGGC CCAGGGGCTG 
AAGCTGGTGG TAGAGACTCT GATGTCATCC CTCAAACCCA TTGGCAACAT TGTGGTCATC 
TGCTGTGCCT TCTTCATCAT TTTTGGAATT CTTGGGGTGC AGCTCTTCAA AGGGAAGTTC 
TTCGTGTGTC AGGGTGAGGA CACCAGGAAC ATCACTAACA AGTCCGACTG TGCTGAGGCC 
AGTTACCGGT GGGTCCGGCA CAAGTACAAC TTTGACAACC TGGGCCAGGC TCTGATGTCC 
CTGTTTGTGC TGGCCTCCAA GGATGGCTGG GTTGACATCA TGTATGATGG ACTGGATGCT 
GTGGGAGTGG ACCAGCAGCC CATCATGAAC CACAACCCTT GGATGCTGCT CTACTTCATC 
TCCTTCCTCC TCATCGTGGC CTTCTTCGTC CTGAACATGT TTGTGGGCGT GGTGGTGGAG 
AACTTCCATA AGTGCAGGCA GCACCAGGAG GAGGAGGAGG CGCGGCGGCG GGAGGAGAAG 
CGACTAAAGA GGCTGGAGAA AAAGAGAAGG AGTAAGGAGA AGCAGATGGC CGATCTAATG 
TTGGACGATG TAATTGCTTC CGGCAGCTCA GCCAGCGCTG CGTCAGAAGC CCAGTGCAAA 
CCCTACTACT CTGACTACTC GCGCTTCCGG CTCCTCGTCC ACCACCTGTG TACCAGCCAC 
TACCTGGACC TCTTCATCAC TGGTGTCATC GGGCTGAATG TGGTCACGAT GGCCATGGAA 
CATTACCAGC AGCCCCAGAT CCTGGACGAG GCTCTGAAGA TCTGCAACTA CATCTTTACC 
GTCATCTTTG TCTTGGAGTC AGTATTCAAA CTTGTGGCCT TCGGCTTCCG CCGGTTCTTC 
CAGGACAGGT GGAACCAGCT GGACCTGGCT ATTGTGCTTC TGTCCATCAT GGGCATCACG 
CTGGAAGAGA TTGAGGTCAA TGCTTCACTG CCCATCAACC CCACCATCAT CCGTATCATG 
AGGGTGCTCC GCATTGCTCG AGTTCTGAAG CTGTTGAAGA TGGCTGTGGG CATGCGGGCA 
CTGCTGGACA CGGTGATGCA GGCCCTGCCC CAGGTGGGGA ACCTGGGACT TCTCTTCATG 
CTATTATTTT TCATCTTTGC AGCTCTGGGC GTGGAGCTCT TTGGAGACCT GGAGTGTGAT 
GAGACACACC CTTGTGAGGG CTTGGGCCGG CATGCCACCT TTAGGAACTT TGGTATGGCC 
TTTCTGACCC TCTTCCGAGT CTCCACTGGT GACAACTGGA ATGGTATTAT GAAGGACACC 
CTCCGGGACT GTGACCAGGA GTCCACCTGC TACAACACCG TCATCTCACC CATCTACTTC 
GTGTCCTTCG TGCTGACGGC CCAGTTTGTG CTGGTCAACG TGGTCATAGC CGTGCTGATG 
AAGCACCTGG AAGAGAGCAA CAAAGAGGCC AAGGAGGAGG CGGAGTTGGA GGCGGAGCTG 
GAGCTAGAGA TGAAGACACT CAGCCCGCAG CCCCACTCCC CGCTGGGCAG CCCCTTCCTC 
TGGCCTGGGG TGGAAGGTGT CAATAGCCCT GACAGCCCTA AGCCTGGGGC TCCACACACC 
ACGGCCCACA TTGGAGCAGC CTCTTCAGGC TTCTCCCTTG AGCACCCCAC GGACAGACAG 
CTGTTTGACA CCATCTCCCT GCTGATCCAG GGCTCCCTGG AGGGGGAGCT GAAGCTGATG 
GACGAGCTGG CAGGCCCAGG GGGCCAGCCC TCTGCCTTCC CTTCCGCCCC CAGCCCGGGC 
GACTCCGACC CACAGATCCC TCTAGCTGAG ATGGAGGCTC TGTCTCTGAC GTCAGAGATT 
GTGTCTGAAC CGTCCTGCTC TCTAGCTCTG ACGGATGACT CTTTGCCTGA TGACACTCAC 
ACACTCTTAC TTAGTGCCCT GGAGAGCAAT ATGGTACCTC ACACTGAGGA GGGGCCAGTC 
CCCCTAGGAC CAGACCTGCT GACTGTGAGG AAGTCTGGTG TCAGCCGGAC ACACTCTCTG 
CCCAATGACA GCTACATGTG CCGCAATGGG AGCACTGCCG AGAGATCCCT AGGACACAGG 
GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC TCCATCTTGT CTGTTCACTC CCAACCAGCA 
GACACCAGCT GCATCCTACA GCTTCCCAAA GATGCACACT ATCTGCTCCA GCCTCATGGG 
GCTCCCACCT GGGGCGCCAT CCCTAAACTA CCCCCACCTG GCCGCTCCCC TCTGGCTCAG 
AGGCCTCTCA GGCGCCAGGC AGCAATAAGG ACTGACTCCC TGGACGTGCA GGGCCTGGGT 
AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA 
TCCTTCTGGG GCGGGTCGAG CATCCAGGTG CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC 
TCCAAGCACA TCCGCCTGCC AGCCCCTTGC CCAGGCCTGG AACCCAGCTG GGCCAAGGAC 
CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGCCCA GCAGTCAGGA AGAACCCCTG TCCCCACGGG ACTTGAAAAA ATGCTACAGT 
GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT GGGTCCTGGC TAGACGAACA GAGGAGACAC 
TCCATCGCTG TCAGCTGCCT GGACAGCGGC TCCCAGCCCC GCCTATGTCC AAGCCCCTCA 
AGCCTCGGGG GCCAACCTCT TGGGGGCCCT GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA 
CCCAGTATCT CTATAGACCC CCCGGAGAGC CAGGGCCCTC GGCCCCCATG CAGTCCTGGC 
GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT GACTCGAAGG ATCCCTCGGC CTCCAGCCCC 
CTTGACAGCA CGGCTGCCTC ACCCTCCCCA AAGAAAGATG CGCTGAGTCT CTCTGGTTTG 
TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248691.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246993.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X9, mRNA.

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CloneID	OMu67273
Clone ID Related Accession (Same CDS sequence)	XM_011248691.3 , XM_011248691.2
Accession Version	XM_011248691.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248691.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGGACAG ACAGCTGTTT 
GACACCATCT CCCTGCTGAT CCAGGGCTCC CTGGAGGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTTCCG CCCCCAGCCC GGGCGACTCC 
GACCCACAGA TCCCTCTAGC TGAGATGGAG GCTCTGTCTC TGACGTCAGA GATTGTGTCT 
GAACCGTCCT GCTCTCTAGC TCTGACGGAT GACTCTTTGC CTGATGACAC TCACACACTC 
TTACTTAGTG CCCTGGAGAG CAATATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246993.1
CDS	773..7876
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248691.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248691.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGGACAG ACAGCTGTTT 
GACACCATCT CCCTGCTGAT CCAGGGCTCC CTGGAGGGGG AGCTGAAGCT GATGGACGAG 
CTGGCAGGCC CAGGGGGCCA GCCCTCTGCC TTCCCTTCCG CCCCCAGCCC GGGCGACTCC 
GACCCACAGA TCCCTCTAGC TGAGATGGAG GCTCTGTCTC TGACGTCAGA GATTGTGTCT 
GAACCGTCCT GCTCTCTAGC TCTGACGGAT GACTCTTTGC CTGATGACAC TCACACACTC 
TTACTTAGTG CCCTGGAGAG CAATATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248692.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246994.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X14, mRNA.

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CloneID	OMu67274
Clone ID Related Accession (Same CDS sequence)	XM_011248692.3 , XM_011248692.2
Accession Version	XM_011248692.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
GACAGACAGC TGTTTGACAC CATCTCCCTG CTGATCCAGG GCTCCCTGGA GGGGGAGCTG 
AAGCTGATGG ACGAGCTGGC AGGCCCAGGG GGCCAGCCCT CTGCCTTCCC TTCCGCCCCC 
AGCCCGGGCG ACTCCGACCC ACAGATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246994.1
CDS	773..7816
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248692.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248692.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
GACAGACAGC TGTTTGACAC CATCTCCCTG CTGATCCAGG GCTCCCTGGA GGGGGAGCTG 
AAGCTGATGG ACGAGCTGGC AGGCCCAGGG GGCCAGCCCT CTGCCTTCCC TTCCGCCCCC 
AGCCCGGGCG ACTCCGACCC ACAGATGGTA CCTCACACTG AGGAGGGGCC AGTCCCCCTA 
GGACCAGACC TGCTGACTGT GAGGAAGTCT GGTGTCAGCC GGACACACTC TCTGCCCAAT 
GACAGCTACA TGTGCCGCAA TGGGAGCACT GCCGAGAGAT CCCTAGGACA CAGGGGCTGG 
GGGCTCCCCA AAGCCCAGTC AGGCTCCATC TTGTCTGTTC ACTCCCAACC AGCAGACACC 
AGCTGCATCC TACAGCTTCC CAAAGATGCA CACTATCTGC TCCAGCCTCA TGGGGCTCCC 
ACCTGGGGCG CCATCCCTAA ACTACCCCCA CCTGGCCGCT CCCCTCTGGC TCAGAGGCCT 
CTCAGGCGCC AGGCAGCAAT AAGGACTGAC TCCCTGGACG TGCAGGGCCT GGGTAGCCGG 
GAAGACCTGT TGTCAGAGGT GAGTGGGCCC TCCTGCCCTC TGACCCGCTC CTCATCCTTC 
TGGGGCGGGT CGAGCATCCA GGTGCAGCAG CGCTCCGGCA GCCAGAGCAA AGTCTCCAAG 
CACATCCGCC TGCCAGCCCC TTGCCCAGGC CTGGAACCCA GCTGGGCCAA GGACCCTCAA 
GAGACCAGAA GCAGCTTAGA GCTGGACACG GAGCTGAGCT GGATTTCAGG AGACCTCCTG 
CCCAGCAGTC AGGAAGAACC CCTGTCCCCA CGGGACTTGA AAAAATGCTA CAGTGTAGAG 
GCCCAGAGCT GCCGGCGCAG GCCTGGGTCC TGGCTAGACG AACAGAGGAG ACACTCCATC 
GCTGTCAGCT GCCTGGACAG CGGCTCCCAG CCCCGCCTAT GTCCAAGCCC CTCAAGCCTC 
GGGGGCCAAC CTCTTGGGGG CCCTGGGAGC CGGCCTAAGA AAAAACTCAG CCCACCCAGT 
ATCTCTATAG ACCCCCCGGA GAGCCAGGGC CCTCGGCCCC CATGCAGTCC TGGCGTCTGC 
CTCAGGAGGA GGGCGCCGGC CAGTGACTCG AAGGATCCCT CGGCCTCCAG CCCCCTTGAC 
AGCACGGCTG CCTCACCCTC CCCAAAGAAA GATGCGCTGA GTCTCTCTGG TTTGTCTTCT 
GACCCAACAG ACCTGGATCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248693.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246995.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X15, mRNA.

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CloneID	OMu67275
Clone ID Related Accession (Same CDS sequence)	XM_011248693.2 , XM_011248693.3
Accession Version	XM_011248693.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
ATCCCTCTAG CTGAGATGGA GGCTCTGTCT CTGACGTCAG AGATTGTGTC TGAACCGTCC 
TGCTCTCTAG CTCTGACGGA TGACTCTTTG CCTGATGACA CTCACACACT CTTACTTAGT 
GCCCTGGAGA GCAATATGGT ACCTCACACT GAGGAGGGGC CAGTCCCCCT AGGACCAGAC 
CTGCTGACTG TGAGGAAGTC TGGTGTCAGC CGGACACACT CTCTGCCCAA TGACAGCTAC 
ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC 
AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT CACTCCCAAC CAGCAGACAC CAGCTGCATC 
CTACAGCTTC CCAAAGATGC ACACTATCTG CTCCAGCCTC ATGGGGCTCC CACCTGGGGC 
GCCATCCCTA AACTACCCCC ACCTGGCCGC TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC 
CAGGCAGCAA TAAGGACTGA CTCCCTGGAC GTGCAGGGCC TGGGTAGCCG GGAAGACCTG 
TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT CTGACCCGCT CCTCATCCTT CTGGGGCGGG 
TCGAGCATCC AGGTGCAGCA GCGCTCCGGC AGCCAGAGCA AAGTCTCCAA GCACATCCGC 
CTGCCAGCCC CTTGCCCAGG CCTGGAACCC AGCTGGGCCA AGGACCCTCA AGAGACCAGA 
AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GCCCAGCAGT 
CAGGAAGAAC CCCTGTCCCC ACGGGACTTG AAAAAATGCT ACAGTGTAGA GGCCCAGAGC 
TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC GAACAGAGGA GACACTCCAT CGCTGTCAGC 
TGCCTGGACA GCGGCTCCCA GCCCCGCCTA TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA 
CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG AAAAAACTCA GCCCACCCAG TATCTCTATA 
GACCCCCCGG AGAGCCAGGG CCCTCGGCCC CCATGCAGTC CTGGCGTCTG CCTCAGGAGG 
AGGGCGCCGG CCAGTGACTC GAAGGATCCC TCGGCCTCCA GCCCCCTTGA CAGCACGGCT 
GCCTCACCCT CCCCAAAGAA AGATGCGCTG AGTCTCTCTG GTTTGTCTTC TGACCCAACA 
GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246995.1
CDS	773..7807
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTIPLAEMEALSLTSEIVSEPSCSLALTDDSLPDDTHTLLLSALESNMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248693.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248693.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
ATCCCTCTAG CTGAGATGGA GGCTCTGTCT CTGACGTCAG AGATTGTGTC TGAACCGTCC 
TGCTCTCTAG CTCTGACGGA TGACTCTTTG CCTGATGACA CTCACACACT CTTACTTAGT 
GCCCTGGAGA GCAATATGGT ACCTCACACT GAGGAGGGGC CAGTCCCCCT AGGACCAGAC 
CTGCTGACTG TGAGGAAGTC TGGTGTCAGC CGGACACACT CTCTGCCCAA TGACAGCTAC 
ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC 
AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT CACTCCCAAC CAGCAGACAC CAGCTGCATC 
CTACAGCTTC CCAAAGATGC ACACTATCTG CTCCAGCCTC ATGGGGCTCC CACCTGGGGC 
GCCATCCCTA AACTACCCCC ACCTGGCCGC TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC 
CAGGCAGCAA TAAGGACTGA CTCCCTGGAC GTGCAGGGCC TGGGTAGCCG GGAAGACCTG 
TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT CTGACCCGCT CCTCATCCTT CTGGGGCGGG 
TCGAGCATCC AGGTGCAGCA GCGCTCCGGC AGCCAGAGCA AAGTCTCCAA GCACATCCGC 
CTGCCAGCCC CTTGCCCAGG CCTGGAACCC AGCTGGGCCA AGGACCCTCA AGAGACCAGA 
AGCAGCTTAG AGCTGGACAC GGAGCTGAGC TGGATTTCAG GAGACCTCCT GCCCAGCAGT 
CAGGAAGAAC CCCTGTCCCC ACGGGACTTG AAAAAATGCT ACAGTGTAGA GGCCCAGAGC 
TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC GAACAGAGGA GACACTCCAT CGCTGTCAGC 
TGCCTGGACA GCGGCTCCCA GCCCCGCCTA TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA 
CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG AAAAAACTCA GCCCACCCAG TATCTCTATA 
GACCCCCCGG AGAGCCAGGG CCCTCGGCCC CCATGCAGTC CTGGCGTCTG CCTCAGGAGG 
AGGGCGCCGG CCAGTGACTC GAAGGATCCC TCGGCCTCCA GCCCCCTTGA CAGCACGGCT 
GCCTCACCCT CCCCAAAGAA AGATGCGCTG AGTCTCTCTG GTTTGTCTTC TGACCCAACA 
GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248694.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246996.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X17, mRNA.

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CloneID	OMu67276
Clone ID Related Accession (Same CDS sequence)	XM_011248694.2 , XM_011248694.3
Accession Version	XM_011248694.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248694.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GAAGCCCAGT GCAAACCCTA CTACTCTGAC TACTCGCGCT TCCGGCTCCT CGTCCACCAC 
CTGTGTACCA GCCACTACCT GGACCTCTTC ATCACTGGTG TCATCGGGCT GAATGTGGTC 
ACGATGGCCA TGGAACATTA CCAGCAGCCC CAGATCCTGG ACGAGGCTCT GAAGATCTGC 
AACTACATCT TTACCGTCAT CTTTGTCTTG GAGTCAGTAT TCAAACTTGT GGCCTTCGGC 
TTCCGCCGGT TCTTCCAGGA CAGGTGGAAC CAGCTGGACC TGGCTATTGT GCTTCTGTCC 
ATCATGGGCA TCACGCTGGA AGAGATTGAG GTCAATGCTT CACTGCCCAT CAACCCCACC 
ATCATCCGTA TCATGAGGGT GCTCCGCATT GCTCGAGTTC TGAAGCTGTT GAAGATGGCT 
GTGGGCATGC GGGCACTGCT GGACACGGTG ATGCAGGCCC TGCCCCAGGT GGGGAACCTG 
GGACTTCTCT TCATGCTATT ATTTTTCATC TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA 
GACCTGGAGT GTGATGAGAC ACACCCTTGT GAGGGCTTGG GCCGGCATGC CACCTTTAGG 
AACTTTGGTA TGGCCTTTCT GACCCTCTTC CGAGTCTCCA CTGGTGACAA CTGGAATGGT 
ATTATGAAGG ACACCCTCCG GGACTGTGAC CAGGAGTCCA CCTGCTACAA CACCGTCATC 
TCACCCATCT ACTTCGTGTC CTTCGTGCTG ACGGCCCAGT TTGTGCTGGT CAACGTGGTC 
ATAGCCGTGC TGATGAAGCA CCTGGAAGAG AGCAACAAAG AGGCCAAGGA GGAGGCGGAG 
TTGGAGGCGG AGCTGGAGCT AGAGATGAAG ACACTCAGCC CGCAGCCCCA CTCCCCGCTG 
GGCAGCCCCT TCCTCTGGCC TGGGGTGGAA GGTGTCAATA GCCCTGACAG CCCTAAGCCT 
GGGGCTCCAC ACACCACGGC CCACATTGGA GCAGCCTCTT CAGGCTTCTC CCTTGAGCAC 
CCCACGGACA GACAGCTGTT TGACACCATC TCCCTGCTGA TCCAGGGCTC CCTGGAGGGG 
GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTTCC 
GCCCCCAGCC CGGGCGACTC CGACCCACAG ATGGTACCTC ACACTGAGGA GGGGCCAGTC 
CCCCTAGGAC CAGACCTGCT GACTGTGAGG AAGTCTGGTG TCAGCCGGAC ACACTCTCTG 
CCCAATGACA GCTACATGTG CCGCAATGGG AGCACTGCCG AGAGATCCCT AGGACACAGG 
GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC TCCATCTTGT CTGTTCACTC CCAACCAGCA 
GACACCAGCT GCATCCTACA GCTTCCCAAA GATGCACACT ATCTGCTCCA GCCTCATGGG 
GCTCCCACCT GGGGCGCCAT CCCTAAACTA CCCCCACCTG GCCGCTCCCC TCTGGCTCAG 
AGGCCTCTCA GGCGCCAGGC AGCAATAAGG ACTGACTCCC TGGACGTGCA GGGCCTGGGT 
AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA 
TCCTTCTGGG GCGGGTCGAG CATCCAGGTG CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC 
TCCAAGCACA TCCGCCTGCC AGCCCCTTGC CCAGGCCTGG AACCCAGCTG GGCCAAGGAC 
CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGCCCA GCAGTCAGGA AGAACCCCTG TCCCCACGGG ACTTGAAAAA ATGCTACAGT 
GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT GGGTCCTGGC TAGACGAACA GAGGAGACAC 
TCCATCGCTG TCAGCTGCCT GGACAGCGGC TCCCAGCCCC GCCTATGTCC AAGCCCCTCA 
AGCCTCGGGG GCCAACCTCT TGGGGGCCCT GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA 
CCCAGTATCT CTATAGACCC CCCGGAGAGC CAGGGCCCTC GGCCCCCATG CAGTCCTGGC 
GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT GACTCGAAGG ATCCCTCGGC CTCCAGCCCC 
CTTGACAGCA CGGCTGCCTC ACCCTCCCCA AAGAAAGATG CGCTGAGTCT CTCTGGTTTG 
TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246996.1
CDS	773..7762
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTDRQLFDTISLLIQGSLEGELKLMDELAGPGGQPSAFPSAPSPGDSDPQMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248694.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248694.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GAAGCCCAGT GCAAACCCTA CTACTCTGAC TACTCGCGCT TCCGGCTCCT CGTCCACCAC 
CTGTGTACCA GCCACTACCT GGACCTCTTC ATCACTGGTG TCATCGGGCT GAATGTGGTC 
ACGATGGCCA TGGAACATTA CCAGCAGCCC CAGATCCTGG ACGAGGCTCT GAAGATCTGC 
AACTACATCT TTACCGTCAT CTTTGTCTTG GAGTCAGTAT TCAAACTTGT GGCCTTCGGC 
TTCCGCCGGT TCTTCCAGGA CAGGTGGAAC CAGCTGGACC TGGCTATTGT GCTTCTGTCC 
ATCATGGGCA TCACGCTGGA AGAGATTGAG GTCAATGCTT CACTGCCCAT CAACCCCACC 
ATCATCCGTA TCATGAGGGT GCTCCGCATT GCTCGAGTTC TGAAGCTGTT GAAGATGGCT 
GTGGGCATGC GGGCACTGCT GGACACGGTG ATGCAGGCCC TGCCCCAGGT GGGGAACCTG 
GGACTTCTCT TCATGCTATT ATTTTTCATC TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA 
GACCTGGAGT GTGATGAGAC ACACCCTTGT GAGGGCTTGG GCCGGCATGC CACCTTTAGG 
AACTTTGGTA TGGCCTTTCT GACCCTCTTC CGAGTCTCCA CTGGTGACAA CTGGAATGGT 
ATTATGAAGG ACACCCTCCG GGACTGTGAC CAGGAGTCCA CCTGCTACAA CACCGTCATC 
TCACCCATCT ACTTCGTGTC CTTCGTGCTG ACGGCCCAGT TTGTGCTGGT CAACGTGGTC 
ATAGCCGTGC TGATGAAGCA CCTGGAAGAG AGCAACAAAG AGGCCAAGGA GGAGGCGGAG 
TTGGAGGCGG AGCTGGAGCT AGAGATGAAG ACACTCAGCC CGCAGCCCCA CTCCCCGCTG 
GGCAGCCCCT TCCTCTGGCC TGGGGTGGAA GGTGTCAATA GCCCTGACAG CCCTAAGCCT 
GGGGCTCCAC ACACCACGGC CCACATTGGA GCAGCCTCTT CAGGCTTCTC CCTTGAGCAC 
CCCACGGACA GACAGCTGTT TGACACCATC TCCCTGCTGA TCCAGGGCTC CCTGGAGGGG 
GAGCTGAAGC TGATGGACGA GCTGGCAGGC CCAGGGGGCC AGCCCTCTGC CTTCCCTTCC 
GCCCCCAGCC CGGGCGACTC CGACCCACAG ATGGTACCTC ACACTGAGGA GGGGCCAGTC 
CCCCTAGGAC CAGACCTGCT GACTGTGAGG AAGTCTGGTG TCAGCCGGAC ACACTCTCTG 
CCCAATGACA GCTACATGTG CCGCAATGGG AGCACTGCCG AGAGATCCCT AGGACACAGG 
GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC TCCATCTTGT CTGTTCACTC CCAACCAGCA 
GACACCAGCT GCATCCTACA GCTTCCCAAA GATGCACACT ATCTGCTCCA GCCTCATGGG 
GCTCCCACCT GGGGCGCCAT CCCTAAACTA CCCCCACCTG GCCGCTCCCC TCTGGCTCAG 
AGGCCTCTCA GGCGCCAGGC AGCAATAAGG ACTGACTCCC TGGACGTGCA GGGCCTGGGT 
AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA 
TCCTTCTGGG GCGGGTCGAG CATCCAGGTG CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC 
TCCAAGCACA TCCGCCTGCC AGCCCCTTGC CCAGGCCTGG AACCCAGCTG GGCCAAGGAC 
CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG GACACGGAGC TGAGCTGGAT TTCAGGAGAC 
CTCCTGCCCA GCAGTCAGGA AGAACCCCTG TCCCCACGGG ACTTGAAAAA ATGCTACAGT 
GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT GGGTCCTGGC TAGACGAACA GAGGAGACAC 
TCCATCGCTG TCAGCTGCCT GGACAGCGGC TCCCAGCCCC GCCTATGTCC AAGCCCCTCA 
AGCCTCGGGG GCCAACCTCT TGGGGGCCCT GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA 
CCCAGTATCT CTATAGACCC CCCGGAGAGC CAGGGCCCTC GGCCCCCATG CAGTCCTGGC 
GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT GACTCGAAGG ATCCCTCGGC CTCCAGCCCC 
CTTGACAGCA CGGCTGCCTC ACCCTCCCCA AAGAAAGATG CGCTGAGTCT CTCTGGTTTG 
TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248695.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246997.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X22, mRNA.

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CloneID	OMu67277
Clone ID Related Accession (Same CDS sequence)	XM_011248695.2 , XM_011248695.3
Accession Version	XM_011248695.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248695.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
ATGGTACCTC ACACTGAGGA GGGGCCAGTC CCCCTAGGAC CAGACCTGCT GACTGTGAGG 
AAGTCTGGTG TCAGCCGGAC ACACTCTCTG CCCAATGACA GCTACATGTG CCGCAATGGG 
AGCACTGCCG AGAGATCCCT AGGACACAGG GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC 
TCCATCTTGT CTGTTCACTC CCAACCAGCA GACACCAGCT GCATCCTACA GCTTCCCAAA 
GATGCACACT ATCTGCTCCA GCCTCATGGG GCTCCCACCT GGGGCGCCAT CCCTAAACTA 
CCCCCACCTG GCCGCTCCCC TCTGGCTCAG AGGCCTCTCA GGCGCCAGGC AGCAATAAGG 
ACTGACTCCC TGGACGTGCA GGGCCTGGGT AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT 
GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA TCCTTCTGGG GCGGGTCGAG CATCCAGGTG 
CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC TCCAAGCACA TCCGCCTGCC AGCCCCTTGC 
CCAGGCCTGG AACCCAGCTG GGCCAAGGAC CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA GCAGTCAGGA AGAACCCCTG 
TCCCCACGGG ACTTGAAAAA ATGCTACAGT GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT 
GGGTCCTGGC TAGACGAACA GAGGAGACAC TCCATCGCTG TCAGCTGCCT GGACAGCGGC 
TCCCAGCCCC GCCTATGTCC AAGCCCCTCA AGCCTCGGGG GCCAACCTCT TGGGGGCCCT 
GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA CCCAGTATCT CTATAGACCC CCCGGAGAGC 
CAGGGCCCTC GGCCCCCATG CAGTCCTGGC GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT 
GACTCGAAGG ATCCCTCGGC CTCCAGCCCC CTTGACAGCA CGGCTGCCTC ACCCTCCCCA 
AAGAAAGATG CGCTGAGTCT CTCTGGTTTG TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246997.1
CDS	773..7672
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMADLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248695.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248695.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GATCTAATGT TGGACGATGT AATTGCTTCC GGCAGCTCAG CCAGCGCTGC GTCAGAAGCC 
CAGTGCAAAC CCTACTACTC TGACTACTCG CGCTTCCGGC TCCTCGTCCA CCACCTGTGT 
ACCAGCCACT ACCTGGACCT CTTCATCACT GGTGTCATCG GGCTGAATGT GGTCACGATG 
GCCATGGAAC ATTACCAGCA GCCCCAGATC CTGGACGAGG CTCTGAAGAT CTGCAACTAC 
ATCTTTACCG TCATCTTTGT CTTGGAGTCA GTATTCAAAC TTGTGGCCTT CGGCTTCCGC 
CGGTTCTTCC AGGACAGGTG GAACCAGCTG GACCTGGCTA TTGTGCTTCT GTCCATCATG 
GGCATCACGC TGGAAGAGAT TGAGGTCAAT GCTTCACTGC CCATCAACCC CACCATCATC 
CGTATCATGA GGGTGCTCCG CATTGCTCGA GTTCTGAAGC TGTTGAAGAT GGCTGTGGGC 
ATGCGGGCAC TGCTGGACAC GGTGATGCAG GCCCTGCCCC AGGTGGGGAA CCTGGGACTT 
CTCTTCATGC TATTATTTTT CATCTTTGCA GCTCTGGGCG TGGAGCTCTT TGGAGACCTG 
GAGTGTGATG AGACACACCC TTGTGAGGGC TTGGGCCGGC ATGCCACCTT TAGGAACTTT 
GGTATGGCCT TTCTGACCCT CTTCCGAGTC TCCACTGGTG ACAACTGGAA TGGTATTATG 
AAGGACACCC TCCGGGACTG TGACCAGGAG TCCACCTGCT ACAACACCGT CATCTCACCC 
ATCTACTTCG TGTCCTTCGT GCTGACGGCC CAGTTTGTGC TGGTCAACGT GGTCATAGCC 
GTGCTGATGA AGCACCTGGA AGAGAGCAAC AAAGAGGCCA AGGAGGAGGC GGAGTTGGAG 
GCGGAGCTGG AGCTAGAGAT GAAGACACTC AGCCCGCAGC CCCACTCCCC GCTGGGCAGC 
CCCTTCCTCT GGCCTGGGGT GGAAGGTGTC AATAGCCCTG ACAGCCCTAA GCCTGGGGCT 
CCACACACCA CGGCCCACAT TGGAGCAGCC TCTTCAGGCT TCTCCCTTGA GCACCCCACG 
ATGGTACCTC ACACTGAGGA GGGGCCAGTC CCCCTAGGAC CAGACCTGCT GACTGTGAGG 
AAGTCTGGTG TCAGCCGGAC ACACTCTCTG CCCAATGACA GCTACATGTG CCGCAATGGG 
AGCACTGCCG AGAGATCCCT AGGACACAGG GGCTGGGGGC TCCCCAAAGC CCAGTCAGGC 
TCCATCTTGT CTGTTCACTC CCAACCAGCA GACACCAGCT GCATCCTACA GCTTCCCAAA 
GATGCACACT ATCTGCTCCA GCCTCATGGG GCTCCCACCT GGGGCGCCAT CCCTAAACTA 
CCCCCACCTG GCCGCTCCCC TCTGGCTCAG AGGCCTCTCA GGCGCCAGGC AGCAATAAGG 
ACTGACTCCC TGGACGTGCA GGGCCTGGGT AGCCGGGAAG ACCTGTTGTC AGAGGTGAGT 
GGGCCCTCCT GCCCTCTGAC CCGCTCCTCA TCCTTCTGGG GCGGGTCGAG CATCCAGGTG 
CAGCAGCGCT CCGGCAGCCA GAGCAAAGTC TCCAAGCACA TCCGCCTGCC AGCCCCTTGC 
CCAGGCCTGG AACCCAGCTG GGCCAAGGAC CCTCAAGAGA CCAGAAGCAG CTTAGAGCTG 
GACACGGAGC TGAGCTGGAT TTCAGGAGAC CTCCTGCCCA GCAGTCAGGA AGAACCCCTG 
TCCCCACGGG ACTTGAAAAA ATGCTACAGT GTAGAGGCCC AGAGCTGCCG GCGCAGGCCT 
GGGTCCTGGC TAGACGAACA GAGGAGACAC TCCATCGCTG TCAGCTGCCT GGACAGCGGC 
TCCCAGCCCC GCCTATGTCC AAGCCCCTCA AGCCTCGGGG GCCAACCTCT TGGGGGCCCT 
GGGAGCCGGC CTAAGAAAAA ACTCAGCCCA CCCAGTATCT CTATAGACCC CCCGGAGAGC 
CAGGGCCCTC GGCCCCCATG CAGTCCTGGC GTCTGCCTCA GGAGGAGGGC GCCGGCCAGT 
GACTCGAAGG ATCCCTCGGC CTCCAGCCCC CTTGACAGCA CGGCTGCCTC ACCCTCCCCA 
AAGAAAGATG CGCTGAGTCT CTCTGGTTTG TCTTCTGACC CAACAGACCT GGATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248696.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246998.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X23, mRNA.

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CloneID	OMu67278
Clone ID Related Accession (Same CDS sequence)	XM_011248696.2 , XM_011248696.3
Accession Version	XM_011248696.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248696.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA ATCTAATGTT GGACGATGTA 
ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG TCAGAAGCCC AGTGCAAACC CTACTACTCT 
GACTACTCGC GCTTCCGGCT CCTCGTCCAC CACCTGTGTA CCAGCCACTA CCTGGACCTC 
TTCATCACTG GTGTCATCGG GCTGAATGTG GTCACGATGG CCATGGAACA TTACCAGCAG 
CCCCAGATCC TGGACGAGGC TCTGAAGATC TGCAACTACA TCTTTACCGT CATCTTTGTC 
TTGGAGTCAG TATTCAAACT TGTGGCCTTC GGCTTCCGCC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCTAT TGTGCTTCTG TCCATCATGG GCATCACGCT GGAAGAGATT 
GAGGTCAATG CTTCACTGCC CATCAACCCC ACCATCATCC GTATCATGAG GGTGCTCCGC 
ATTGCTCGAG TTCTGAAGCT GTTGAAGATG GCTGTGGGCA TGCGGGCACT GCTGGACACG 
GTGATGCAGG CCCTGCCCCA GGTGGGGAAC CTGGGACTTC TCTTCATGCT ATTATTTTTC 
ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACACACCCT 
TGTGAGGGCT TGGGCCGGCA TGCCACCTTT AGGAACTTTG GTATGGCCTT TCTGACCCTC 
TTCCGAGTCT CCACTGGTGA CAACTGGAAT GGTATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACCTGCTA CAACACCGTC ATCTCACCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTCATAGCCG TGCTGATGAA GCACCTGGAA 
GAGAGCAACA AAGAGGCCAA GGAGGAGGCG GAGTTGGAGG CGGAGCTGGA GCTAGAGATG 
AAGACACTCA GCCCGCAGCC CCACTCCCCG CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG 
GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG CCTGGGGCTC CACACACCAC GGCCCACATT 
GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG CACCCCACGA TGGTACCTCA CACTGAGGAG 
GGGCCAGTCC CCCTAGGACC AGACCTGCTG ACTGTGAGGA AGTCTGGTGT CAGCCGGACA 
CACTCTCTGC CCAATGACAG CTACATGTGC CGCAATGGGA GCACTGCCGA GAGATCCCTA 
GGACACAGGG GCTGGGGGCT CCCCAAAGCC CAGTCAGGCT CCATCTTGTC TGTTCACTCC 
CAACCAGCAG ACACCAGCTG CATCCTACAG CTTCCCAAAG ATGCACACTA TCTGCTCCAG 
CCTCATGGGG CTCCCACCTG GGGCGCCATC CCTAAACTAC CCCCACCTGG CCGCTCCCCT 
CTGGCTCAGA GGCCTCTCAG GCGCCAGGCA GCAATAAGGA CTGACTCCCT GGACGTGCAG 
GGCCTGGGTA GCCGGGAAGA CCTGTTGTCA GAGGTGAGTG GGCCCTCCTG CCCTCTGACC 
CGCTCCTCAT CCTTCTGGGG CGGGTCGAGC ATCCAGGTGC AGCAGCGCTC CGGCAGCCAG 
AGCAAAGTCT CCAAGCACAT CCGCCTGCCA GCCCCTTGCC CAGGCCTGGA ACCCAGCTGG 
GCCAAGGACC CTCAAGAGAC CAGAAGCAGC TTAGAGCTGG ACACGGAGCT GAGCTGGATT 
TCAGGAGACC TCCTGCCCAG CAGTCAGGAA GAACCCCTGT CCCCACGGGA CTTGAAAAAA 
TGCTACAGTG TAGAGGCCCA GAGCTGCCGG CGCAGGCCTG GGTCCTGGCT AGACGAACAG 
AGGAGACACT CCATCGCTGT CAGCTGCCTG GACAGCGGCT CCCAGCCCCG CCTATGTCCA 
AGCCCCTCAA GCCTCGGGGG CCAACCTCTT GGGGGCCCTG GGAGCCGGCC TAAGAAAAAA 
CTCAGCCCAC CCAGTATCTC TATAGACCCC CCGGAGAGCC AGGGCCCTCG GCCCCCATGC 
AGTCCTGGCG TCTGCCTCAG GAGGAGGGCG CCGGCCAGTG ACTCGAAGGA TCCCTCGGCC 
TCCAGCCCCC TTGACAGCAC GGCTGCCTCA CCCTCCCCAA AGAAAGATGC GCTGAGTCTC 
TCTGGTTTGT CTTCTGACCC AACAGACCTG GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246998.1
CDS	773..7651
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248696.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248696.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA ATCTAATGTT GGACGATGTA 
ATTGCTTCCG GCAGCTCAGC CAGCGCTGCG TCAGAAGCCC AGTGCAAACC CTACTACTCT 
GACTACTCGC GCTTCCGGCT CCTCGTCCAC CACCTGTGTA CCAGCCACTA CCTGGACCTC 
TTCATCACTG GTGTCATCGG GCTGAATGTG GTCACGATGG CCATGGAACA TTACCAGCAG 
CCCCAGATCC TGGACGAGGC TCTGAAGATC TGCAACTACA TCTTTACCGT CATCTTTGTC 
TTGGAGTCAG TATTCAAACT TGTGGCCTTC GGCTTCCGCC GGTTCTTCCA GGACAGGTGG 
AACCAGCTGG ACCTGGCTAT TGTGCTTCTG TCCATCATGG GCATCACGCT GGAAGAGATT 
GAGGTCAATG CTTCACTGCC CATCAACCCC ACCATCATCC GTATCATGAG GGTGCTCCGC 
ATTGCTCGAG TTCTGAAGCT GTTGAAGATG GCTGTGGGCA TGCGGGCACT GCTGGACACG 
GTGATGCAGG CCCTGCCCCA GGTGGGGAAC CTGGGACTTC TCTTCATGCT ATTATTTTTC 
ATCTTTGCAG CTCTGGGCGT GGAGCTCTTT GGAGACCTGG AGTGTGATGA GACACACCCT 
TGTGAGGGCT TGGGCCGGCA TGCCACCTTT AGGAACTTTG GTATGGCCTT TCTGACCCTC 
TTCCGAGTCT CCACTGGTGA CAACTGGAAT GGTATTATGA AGGACACCCT CCGGGACTGT 
GACCAGGAGT CCACCTGCTA CAACACCGTC ATCTCACCCA TCTACTTCGT GTCCTTCGTG 
CTGACGGCCC AGTTTGTGCT GGTCAACGTG GTCATAGCCG TGCTGATGAA GCACCTGGAA 
GAGAGCAACA AAGAGGCCAA GGAGGAGGCG GAGTTGGAGG CGGAGCTGGA GCTAGAGATG 
AAGACACTCA GCCCGCAGCC CCACTCCCCG CTGGGCAGCC CCTTCCTCTG GCCTGGGGTG 
GAAGGTGTCA ATAGCCCTGA CAGCCCTAAG CCTGGGGCTC CACACACCAC GGCCCACATT 
GGAGCAGCCT CTTCAGGCTT CTCCCTTGAG CACCCCACGA TGGTACCTCA CACTGAGGAG 
GGGCCAGTCC CCCTAGGACC AGACCTGCTG ACTGTGAGGA AGTCTGGTGT CAGCCGGACA 
CACTCTCTGC CCAATGACAG CTACATGTGC CGCAATGGGA GCACTGCCGA GAGATCCCTA 
GGACACAGGG GCTGGGGGCT CCCCAAAGCC CAGTCAGGCT CCATCTTGTC TGTTCACTCC 
CAACCAGCAG ACACCAGCTG CATCCTACAG CTTCCCAAAG ATGCACACTA TCTGCTCCAG 
CCTCATGGGG CTCCCACCTG GGGCGCCATC CCTAAACTAC CCCCACCTGG CCGCTCCCCT 
CTGGCTCAGA GGCCTCTCAG GCGCCAGGCA GCAATAAGGA CTGACTCCCT GGACGTGCAG 
GGCCTGGGTA GCCGGGAAGA CCTGTTGTCA GAGGTGAGTG GGCCCTCCTG CCCTCTGACC 
CGCTCCTCAT CCTTCTGGGG CGGGTCGAGC ATCCAGGTGC AGCAGCGCTC CGGCAGCCAG 
AGCAAAGTCT CCAAGCACAT CCGCCTGCCA GCCCCTTGCC CAGGCCTGGA ACCCAGCTGG 
GCCAAGGACC CTCAAGAGAC CAGAAGCAGC TTAGAGCTGG ACACGGAGCT GAGCTGGATT 
TCAGGAGACC TCCTGCCCAG CAGTCAGGAA GAACCCCTGT CCCCACGGGA CTTGAAAAAA 
TGCTACAGTG TAGAGGCCCA GAGCTGCCGG CGCAGGCCTG GGTCCTGGCT AGACGAACAG 
AGGAGACACT CCATCGCTGT CAGCTGCCTG GACAGCGGCT CCCAGCCCCG CCTATGTCCA 
AGCCCCTCAA GCCTCGGGGG CCAACCTCTT GGGGGCCCTG GGAGCCGGCC TAAGAAAAAA 
CTCAGCCCAC CCAGTATCTC TATAGACCCC CCGGAGAGCC AGGGCCCTCG GCCCCCATGC 
AGTCCTGGCG TCTGCCTCAG GAGGAGGGCG CCGGCCAGTG ACTCGAAGGA TCCCTCGGCC 
TCCAGCCCCC TTGACAGCAC GGCTGCCTCA CCCTCCCCAA AGAAAGATGC GCTGAGTCTC 
TCTGGTTTGT CTTCTGACCC AACAGACCTG GATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248697.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246999.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X25, mRNA.

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CloneID	OMu67279
Clone ID Related Accession (Same CDS sequence)	XM_011248697.3 , XM_011248697.2
Accession Version	XM_011248697.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248697.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GAAGCCCAGT GCAAACCCTA CTACTCTGAC TACTCGCGCT TCCGGCTCCT CGTCCACCAC 
CTGTGTACCA GCCACTACCT GGACCTCTTC ATCACTGGTG TCATCGGGCT GAATGTGGTC 
ACGATGGCCA TGGAACATTA CCAGCAGCCC CAGATCCTGG ACGAGGCTCT GAAGATCTGC 
AACTACATCT TTACCGTCAT CTTTGTCTTG GAGTCAGTAT TCAAACTTGT GGCCTTCGGC 
TTCCGCCGGT TCTTCCAGGA CAGGTGGAAC CAGCTGGACC TGGCTATTGT GCTTCTGTCC 
ATCATGGGCA TCACGCTGGA AGAGATTGAG GTCAATGCTT CACTGCCCAT CAACCCCACC 
ATCATCCGTA TCATGAGGGT GCTCCGCATT GCTCGAGTTC TGAAGCTGTT GAAGATGGCT 
GTGGGCATGC GGGCACTGCT GGACACGGTG ATGCAGGCCC TGCCCCAGGT GGGGAACCTG 
GGACTTCTCT TCATGCTATT ATTTTTCATC TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA 
GACCTGGAGT GTGATGAGAC ACACCCTTGT GAGGGCTTGG GCCGGCATGC CACCTTTAGG 
AACTTTGGTA TGGCCTTTCT GACCCTCTTC CGAGTCTCCA CTGGTGACAA CTGGAATGGT 
ATTATGAAGG ACACCCTCCG GGACTGTGAC CAGGAGTCCA CCTGCTACAA CACCGTCATC 
TCACCCATCT ACTTCGTGTC CTTCGTGCTG ACGGCCCAGT TTGTGCTGGT CAACGTGGTC 
ATAGCCGTGC TGATGAAGCA CCTGGAAGAG AGCAACAAAG AGGCCAAGGA GGAGGCGGAG 
TTGGAGGCGG AGCTGGAGCT AGAGATGAAG ACACTCAGCC CGCAGCCCCA CTCCCCGCTG 
GGCAGCCCCT TCCTCTGGCC TGGGGTGGAA GGTGTCAATA GCCCTGACAG CCCTAAGCCT 
GGGGCTCCAC ACACCACGGC CCACATTGGA GCAGCCTCTT CAGGCTTCTC CCTTGAGCAC 
CCCACGATGG TACCTCACAC TGAGGAGGGG CCAGTCCCCC TAGGACCAGA CCTGCTGACT 
GTGAGGAAGT CTGGTGTCAG CCGGACACAC TCTCTGCCCA ATGACAGCTA CATGTGCCGC 
AATGGGAGCA CTGCCGAGAG ATCCCTAGGA CACAGGGGCT GGGGGCTCCC CAAAGCCCAG 
TCAGGCTCCA TCTTGTCTGT TCACTCCCAA CCAGCAGACA CCAGCTGCAT CCTACAGCTT 
CCCAAAGATG CACACTATCT GCTCCAGCCT CATGGGGCTC CCACCTGGGG CGCCATCCCT 
AAACTACCCC CACCTGGCCG CTCCCCTCTG GCTCAGAGGC CTCTCAGGCG CCAGGCAGCA 
ATAAGGACTG ACTCCCTGGA CGTGCAGGGC CTGGGTAGCC GGGAAGACCT GTTGTCAGAG 
GTGAGTGGGC CCTCCTGCCC TCTGACCCGC TCCTCATCCT TCTGGGGCGG GTCGAGCATC 
CAGGTGCAGC AGCGCTCCGG CAGCCAGAGC AAAGTCTCCA AGCACATCCG CCTGCCAGCC 
CCTTGCCCAG GCCTGGAACC CAGCTGGGCC AAGGACCCTC AAGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCAGCAG TCAGGAAGAA 
CCCCTGTCCC CACGGGACTT GAAAAAATGC TACAGTGTAG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGGGT CCTGGCTAGA CGAACAGAGG AGACACTCCA TCGCTGTCAG CTGCCTGGAC 
AGCGGCTCCC AGCCCCGCCT ATGTCCAAGC CCCTCAAGCC TCGGGGGCCA ACCTCTTGGG 
GGCCCTGGGA GCCGGCCTAA GAAAAAACTC AGCCCACCCA GTATCTCTAT AGACCCCCCG 
GAGAGCCAGG GCCCTCGGCC CCCATGCAGT CCTGGCGTCT GCCTCAGGAG GAGGGCGCCG 
GCCAGTGACT CGAAGGATCC CTCGGCCTCC AGCCCCCTTG ACAGCACGGC TGCCTCACCC 
TCCCCAAAGA AAGATGCGCT GAGTCTCTCT GGTTTGTCTT CTGACCCAAC AGACCTGGAT 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246999.1
CDS	773..7618
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248697.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248697.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA GTAAGGAGAA GCAGATGGCC 
GAAGCCCAGT GCAAACCCTA CTACTCTGAC TACTCGCGCT TCCGGCTCCT CGTCCACCAC 
CTGTGTACCA GCCACTACCT GGACCTCTTC ATCACTGGTG TCATCGGGCT GAATGTGGTC 
ACGATGGCCA TGGAACATTA CCAGCAGCCC CAGATCCTGG ACGAGGCTCT GAAGATCTGC 
AACTACATCT TTACCGTCAT CTTTGTCTTG GAGTCAGTAT TCAAACTTGT GGCCTTCGGC 
TTCCGCCGGT TCTTCCAGGA CAGGTGGAAC CAGCTGGACC TGGCTATTGT GCTTCTGTCC 
ATCATGGGCA TCACGCTGGA AGAGATTGAG GTCAATGCTT CACTGCCCAT CAACCCCACC 
ATCATCCGTA TCATGAGGGT GCTCCGCATT GCTCGAGTTC TGAAGCTGTT GAAGATGGCT 
GTGGGCATGC GGGCACTGCT GGACACGGTG ATGCAGGCCC TGCCCCAGGT GGGGAACCTG 
GGACTTCTCT TCATGCTATT ATTTTTCATC TTTGCAGCTC TGGGCGTGGA GCTCTTTGGA 
GACCTGGAGT GTGATGAGAC ACACCCTTGT GAGGGCTTGG GCCGGCATGC CACCTTTAGG 
AACTTTGGTA TGGCCTTTCT GACCCTCTTC CGAGTCTCCA CTGGTGACAA CTGGAATGGT 
ATTATGAAGG ACACCCTCCG GGACTGTGAC CAGGAGTCCA CCTGCTACAA CACCGTCATC 
TCACCCATCT ACTTCGTGTC CTTCGTGCTG ACGGCCCAGT TTGTGCTGGT CAACGTGGTC 
ATAGCCGTGC TGATGAAGCA CCTGGAAGAG AGCAACAAAG AGGCCAAGGA GGAGGCGGAG 
TTGGAGGCGG AGCTGGAGCT AGAGATGAAG ACACTCAGCC CGCAGCCCCA CTCCCCGCTG 
GGCAGCCCCT TCCTCTGGCC TGGGGTGGAA GGTGTCAATA GCCCTGACAG CCCTAAGCCT 
GGGGCTCCAC ACACCACGGC CCACATTGGA GCAGCCTCTT CAGGCTTCTC CCTTGAGCAC 
CCCACGATGG TACCTCACAC TGAGGAGGGG CCAGTCCCCC TAGGACCAGA CCTGCTGACT 
GTGAGGAAGT CTGGTGTCAG CCGGACACAC TCTCTGCCCA ATGACAGCTA CATGTGCCGC 
AATGGGAGCA CTGCCGAGAG ATCCCTAGGA CACAGGGGCT GGGGGCTCCC CAAAGCCCAG 
TCAGGCTCCA TCTTGTCTGT TCACTCCCAA CCAGCAGACA CCAGCTGCAT CCTACAGCTT 
CCCAAAGATG CACACTATCT GCTCCAGCCT CATGGGGCTC CCACCTGGGG CGCCATCCCT 
AAACTACCCC CACCTGGCCG CTCCCCTCTG GCTCAGAGGC CTCTCAGGCG CCAGGCAGCA 
ATAAGGACTG ACTCCCTGGA CGTGCAGGGC CTGGGTAGCC GGGAAGACCT GTTGTCAGAG 
GTGAGTGGGC CCTCCTGCCC TCTGACCCGC TCCTCATCCT TCTGGGGCGG GTCGAGCATC 
CAGGTGCAGC AGCGCTCCGG CAGCCAGAGC AAAGTCTCCA AGCACATCCG CCTGCCAGCC 
CCTTGCCCAG GCCTGGAACC CAGCTGGGCC AAGGACCCTC AAGAGACCAG AAGCAGCTTA 
GAGCTGGACA CGGAGCTGAG CTGGATTTCA GGAGACCTCC TGCCCAGCAG TCAGGAAGAA 
CCCCTGTCCC CACGGGACTT GAAAAAATGC TACAGTGTAG AGGCCCAGAG CTGCCGGCGC 
AGGCCTGGGT CCTGGCTAGA CGAACAGAGG AGACACTCCA TCGCTGTCAG CTGCCTGGAC 
AGCGGCTCCC AGCCCCGCCT ATGTCCAAGC CCCTCAAGCC TCGGGGGCCA ACCTCTTGGG 
GGCCCTGGGA GCCGGCCTAA GAAAAAACTC AGCCCACCCA GTATCTCTAT AGACCCCCCG 
GAGAGCCAGG GCCCTCGGCC CCCATGCAGT CCTGGCGTCT GCCTCAGGAG GAGGGCGCCG 
GCCAGTGACT CGAAGGATCC CTCGGCCTCC AGCCCCCTTG ACAGCACGGC TGCCTCACCC 
TCCCCAAAGA AAGATGCGCT GAGTCTCTCT GGTTTGTCTT CTGACCCAAC AGACCTGGAT 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1g ( XM_011248698.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247000.1 Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X27, mRNA.

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CloneID	OMu67280
Clone ID Related Accession (Same CDS sequence)	XM_011248698.3 , XM_011248698.2
Accession Version	XM_011248698.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248698.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGATGGT ACCTCACACT 
GAGGAGGGGC CAGTCCCCCT AGGACCAGAC CTGCTGACTG TGAGGAAGTC TGGTGTCAGC 
CGGACACACT CTCTGCCCAA TGACAGCTAC ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA 
TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT 
CACTCCCAAC CAGCAGACAC CAGCTGCATC CTACAGCTTC CCAAAGATGC ACACTATCTG 
CTCCAGCCTC ATGGGGCTCC CACCTGGGGC GCCATCCCTA AACTACCCCC ACCTGGCCGC 
TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC CAGGCAGCAA TAAGGACTGA CTCCCTGGAC 
GTGCAGGGCC TGGGTAGCCG GGAAGACCTG TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT 
CTGACCCGCT CCTCATCCTT CTGGGGCGGG TCGAGCATCC AGGTGCAGCA GCGCTCCGGC 
AGCCAGAGCA AAGTCTCCAA GCACATCCGC CTGCCAGCCC CTTGCCCAGG CCTGGAACCC 
AGCTGGGCCA AGGACCCTCA AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCAGCAGT CAGGAAGAAC CCCTGTCCCC ACGGGACTTG 
AAAAAATGCT ACAGTGTAGA GGCCCAGAGC TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC 
GAACAGAGGA GACACTCCAT CGCTGTCAGC TGCCTGGACA GCGGCTCCCA GCCCCGCCTA 
TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG 
AAAAAACTCA GCCCACCCAG TATCTCTATA GACCCCCCGG AGAGCCAGGG CCCTCGGCCC 
CCATGCAGTC CTGGCGTCTG CCTCAGGAGG AGGGCGCCGG CCAGTGACTC GAAGGATCCC 
TCGGCCTCCA GCCCCCTTGA CAGCACGGCT GCCTCACCCT CCCCAAAGAA AGATGCGCTG 
AGTCTCTCTG GTTTGTCTTC TGACCCAACA GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247000.1
CDS	773..7597
Translation	MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGESVGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSGQLSCIQLPVNSQGGDATKSESEPDFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLSSDPTDLDP

Target ORF information:

RefSeq Version	XM_011248698.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248698.2

ORF Insert Sequence:

ATGGATGAGG AAGAGGATGG AGCGGGCGCC GAGGAGTCGG GACAGCCCCG GAGCTTCACG 
CAGCTCAACG ACCTGTCTGG GGCCGGGGGC CGGCAGGGGC CGGGGTCGAC GGAAAAGGAC 
CCGGGCAGCG CGGACTCCGA GGCGGAGGGG CTGCCCTACC CGGCGCTGGC CCCGGTGGTT 
TTCTTCTACT TGAGCCAGGA CAGCCGCCCG CGGAGCTGGT GTCTCCGCAC GGTCTGTAAC 
CCGTGGTTCG AGCGAGTCAG CATGCTGGTT ATTCTCCTCA ACTGTGTGAC TCTGGGTATG 
TTCAGGCCGT GTGAGGACAT TGCCTGTGAC TCCCAACGCT GCCGGATCCT GCAGGCCTTC 
GACGACTTCA TCTTTGCCTT CTTTGCTGTG GAAATGGTGG TGAAGATGGT CGCTTTGGGT 
ATCTTTGGGA AGAAATGTTA CCTGGGAGAC ACTTGGAACC GGCTTGACTT TTTTATCGTC 
ATTGCTGGGA TGCTGGAGTA CTCGCTGGAC CTGCAGAATG TCAGCTTCTC CGCAGTCAGG 
ACAGTCCGTG TGCTGCGACC GCTCAGGGCC ATTAACCGGG TGCCCAGCAT GCGCATTCTC 
GTCACATTAC TGCTGGATAC CTTGCCTATG CTGGGCAATG TCCTGCTGCT CTGTTTCTTC 
GTCTTTTTCA TCTTTGGCAT CGTTGGTGTT CAGCTGTGGG CAGGGCTACT TCGAAACCGA 
TGCTTCCTCC CTGAGAATTT CAGCCTCCCG CTGAGTGTGG ACTTGGAGCC TTACTACCAG 
ACAGAGAATG AGGACGAGAG CCCCTTCATC TGCTCTCAGC CTCGGGAGAA CGGCATGCGA 
TCCTGCAGGA GCGTGCCCAC ACTGCGCGGG GAAGGCGGTG GTGGTCCACC CTGCGGTCTG 
GACTATGAGG CCTATAACAG TTCCAGCAAT ACCACCTGTG TCAACTGGAA CCAATACTAT 
ACCAACTGCT CTGCAGGCGA GCACAACCCC TTCAAAGGCG CCATCAACTT TGACAACATT 
GGCTATGCCT GGATCGCCAT CTTCCAGGTC ATCACACTGG AGGGCTGGGT CGACATCATG 
TACTTTGTGA TGGATGCTCA TTCCTTCTAC AACTTCATCT ACTTCATTCT TCTCATCATC 
GTGGGCTCCT TCTTCATGAT CAACCTGTGC CTGGTGGTGA TCGCCACACA GTTCTCTGAG 
ACCAAGCAAC GGGAGAGTCA GCTGATGCGG GAGCAGCGTG TACGATTCCT GTCCAATGCT 
AGCACCCTGG CAAGCTTCTC TGAGCCAGGC AGCTGCTATG AGGAGCTTCT CAAGTACCTG 
GTGTACATCC TCCGCAAAGC AGCCCGCAGG CTGGCCCAGG TCTCTAGGGC TGTAGGCGTG 
CGGGCTGGGT TGCTCAGCAG CCCAGTGGCC CGTGGTGGGC AGGAGCCCCA GCCCAGTGGC 
AGCTGCTCTC GTTCACACCG TCGTCTGTCT GTCCACCACC TGGTCCACCA CCATCACCAC 
CACCATCACC ACTACCACCT GGGTAATGGG ACGCTCAGAG TTCCCCGGGC CAGCCCAGAG 
ATCCAGGACA GGGATGCCAA TGGGTCCCGC TGGCTCATGC TGCCACCACC CTCTACCCCC 
ACTCCCTCTG GGGGCCCTCC GAGGGGTGCG GAGTCTGTAC ACAGCTTCTA CCATGCTGAC 
TGCCACTTGG AGCCAGTCCG TTGCCAGGCG CCCCCTCCCA GGTCCCCATC GGAGGCATCT 
GGCAGGACTG TGGGTAGTGG GAAGGTATAC CCCACTGTGC ATACCAGCCC TCCACCAGAG 
ATGCTGAAGG ATAAGGCACT AGTGGAGGTG GCCCCCAGCC CTGGGCCCCC CACCCTCACC 
AGCTTCAACA TCCCACCTGG GCCCTTCAGC TCCATGCACA AGCTCCTGGA GACACAGAGT 
ACGGGTGAGA GCGTGGGAGC CTGCCATAGC TCCTGCAAAA TCTCCAGCCC TTGCTCCAAG 
GCAGACAGTG GAGCCTGTGG GCCGGACAGT TGTCCCTACT GTGCCCGGAC AGGAGCAGGG 
GAGCCAGAGT CCGCTGACCA TGAAATGCCT GACTCAGACA GTGAGGCTGT GTATGAGTTC 
ACACAGGACG CTCAGCACAG CGACCTCCGG GATCCCCACA GACGGCGACG GCCGAGCCTG 
GGCCCAGATG CAGAGCCTAG TTCTGTGCTG GCCTTCTGGA GGCTGATCTG CGACACATTC 
CGGAAGATCG TAGATAGCAA ATACTTTGGC CGGGGAATCA TGATCGCTAT CCTGGTCAAT 
ACCCTCAGCA TGGGCATCGA GTACCACGAG CAGCCCGAGG AGCTCACCAA CGCCCTGGAA 
ATCAGCAACA TCGTCTTCAC CAGCCTCTTC GCCTTGGAGA TGCTGCTGAA GCTGCTCGTC 
TACGGTCCCT TCGGCTACAT TAAGAATCCC TACAACATCT TTGATGGCGT CATTGTGGTC 
ATCAGCGTGT GGGAGATTGT GGGCCAGCAG GGAGGTGGCC TGTCGGTGCT GCGGACCTTC 
CGCCTGATGC GGGTGCTGAA GCTAGTTCGC TTCCTGCCGG CACTGCAGCG GCAGCTCGTG 
GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTCTTCATC 
TTCATCTTCA GCATCCTGGG CATGCACCTT TTTGGTTGCA AGTTCGCATC TGAACGGGAT 
GGGGACACGT TGCCAGACCG GAAGAATTTT GACTCCCTGC TCTGGGCCAT TGTCACTGTC 
TTTCAGATTC TGACTCAGGA AGACTGGAAT AAAGTCCTTT ACAACGGCAT GGCCTCCACG 
TCATCTTGGG CTGCTCTTTA CTTCATCGCC CTCATGACTT TTGGCAACTA CGTGCTCTTT 
AACCTGCTTG TCGCCATTCT CGTGGAGGGT TTCCAGGCAG AGGAAATCGG CAAACGGGAA 
GATACGAGTG GACAGTTAAG CTGTATTCAG CTGCCTGTCA ACTCCCAGGG GGGAGATGCC 
ACCAAGTCTG AGTCAGAGCC TGATTTCTTT TCGCCCAGTG TGGATGGTGA TGGGGACAGG 
AAGAAGCGCT TGGCCCTGGT GGCCTTGGGA GAACACTCGG AACTACGAAA GAGCCTTTTG 
CCACCTCTCA TCATCCACAC AGCTGCTACA CCGATGTCAC TGCCCAAGAG CTCCAGCACA 
GGTGTGGGGG AAGCACTGGG CTCTGGCTCT CGCCGCACCA GTAGCAGTGG GTCCGCTGAG 
CCTGGAACTG CCCATCATGA GATGAAATCA CCGCCAAGTG CCCGAAGCTC CCCGCACAGT 
CCCTGGAGCG CAGCAAGCAG CTGGACCAGC AGGCGCTCCA GCCGGAACAG CCTGGGCCGG 
GCCCCCAGCC TGAAGCGTAG GAGCCCAAGC GGGGAGCGGA GGTCCCTGCT GTCTGGAGAG 
GGTCAGGAGA GCCAGGATGA GGAGGAGAGT TCAGAAGAGG ACCGGGCCAG CCCAGCAGGC 
AGTGATCATC GCCACAGGGG TTCCTTGGAA CGTGAGGCCA AGAGTTCCTT TGACCTGCCT 
GACACCCTGC AGGTGCCCGG GCTTCATCGA ACAGCCAGCG GTCGGAGCTC TGCCTCTGAA 
CACCAAGACT GTAATGGCAA GTCGGCTTCA GGGCGTTTGG CCCGCACCCT GCGGGCTGAT 
GACCCCCCAC TGGATGGGGA TGATGGCGAT GATGAGGGCA ACCTGAGCAA AGGGGAACGC 
TTACGAGCCT GGGTCCGAGC CCGGCTCCCT GCCTGTTGCC GAGAGCGAGA TTCCTGGTCT 
GCCTATATCT TCCCTCCCCA GTCAAGGTTT CGTCTCCTGT GTCACCGGAT CATCACCCAC 
AAGATGTTTG ACCATGTGGT CCTCGTCATC ATCTTCCTCA ACTGTATCAC CATCGCTATG 
GAGCGCCCCA AAATTGACCC CCACAGCGCT GAACGCATCT TCCTGACCCT CTCCAACTAC 
ATCTTCACGG CAGTCTTCCT GGCTGAAATG ACAGTGAAGG TGGTGGCACT GGGCTGGTGC 
TTTGGGGAGC AGGCCTACCT GCGCAGCAGC TGGAACGTGC TGGACGGCTT GCTGGTGCTC 
ATCTCTGTCA TCGACATCCT GGTGTCCATG GTCTCTGACA GCGGCACCAA GATTCTCGGC 
ATGCTGAGGG TGCTGCGGCT GCTGCGGACC CTACGTCCAC TCAGGGTCAT CAGCCGGGCC 
CAGGGGCTGA AGCTGGTGGT AGAGACTCTG ATGTCATCCC TCAAACCCAT TGGCAACATT 
GTGGTCATCT GCTGTGCCTT CTTCATCATT TTTGGAATTC TTGGGGTGCA GCTCTTCAAA 
GGGAAGTTCT TCGTGTGTCA GGGTGAGGAC ACCAGGAACA TCACTAACAA GTCCGACTGT 
GCTGAGGCCA GTTACCGGTG GGTCCGGCAC AAGTACAACT TTGACAACCT GGGCCAGGCT 
CTGATGTCCC TGTTTGTGCT GGCCTCCAAG GATGGCTGGG TTGACATCAT GTATGATGGA 
CTGGATGCTG TGGGAGTGGA CCAGCAGCCC ATCATGAACC ACAACCCTTG GATGCTGCTC 
TACTTCATCT CCTTCCTCCT CATCGTGGCC TTCTTCGTCC TGAACATGTT TGTGGGCGTG 
GTGGTGGAGA ACTTCCATAA GTGCAGGCAG CACCAGGAGG AGGAGGAGGC GCGGCGGCGG 
GAGGAGAAGC GACTAAAGAG GCTGGAGAAA AAGAGAAGGA AAGCCCAGTG CAAACCCTAC 
TACTCTGACT ACTCGCGCTT CCGGCTCCTC GTCCACCACC TGTGTACCAG CCACTACCTG 
GACCTCTTCA TCACTGGTGT CATCGGGCTG AATGTGGTCA CGATGGCCAT GGAACATTAC 
CAGCAGCCCC AGATCCTGGA CGAGGCTCTG AAGATCTGCA ACTACATCTT TACCGTCATC 
TTTGTCTTGG AGTCAGTATT CAAACTTGTG GCCTTCGGCT TCCGCCGGTT CTTCCAGGAC 
AGGTGGAACC AGCTGGACCT GGCTATTGTG CTTCTGTCCA TCATGGGCAT CACGCTGGAA 
GAGATTGAGG TCAATGCTTC ACTGCCCATC AACCCCACCA TCATCCGTAT CATGAGGGTG 
CTCCGCATTG CTCGAGTTCT GAAGCTGTTG AAGATGGCTG TGGGCATGCG GGCACTGCTG 
GACACGGTGA TGCAGGCCCT GCCCCAGGTG GGGAACCTGG GACTTCTCTT CATGCTATTA 
TTTTTCATCT TTGCAGCTCT GGGCGTGGAG CTCTTTGGAG ACCTGGAGTG TGATGAGACA 
CACCCTTGTG AGGGCTTGGG CCGGCATGCC ACCTTTAGGA ACTTTGGTAT GGCCTTTCTG 
ACCCTCTTCC GAGTCTCCAC TGGTGACAAC TGGAATGGTA TTATGAAGGA CACCCTCCGG 
GACTGTGACC AGGAGTCCAC CTGCTACAAC ACCGTCATCT CACCCATCTA CTTCGTGTCC 
TTCGTGCTGA CGGCCCAGTT TGTGCTGGTC AACGTGGTCA TAGCCGTGCT GATGAAGCAC 
CTGGAAGAGA GCAACAAAGA GGCCAAGGAG GAGGCGGAGT TGGAGGCGGA GCTGGAGCTA 
GAGATGAAGA CACTCAGCCC GCAGCCCCAC TCCCCGCTGG GCAGCCCCTT CCTCTGGCCT 
GGGGTGGAAG GTGTCAATAG CCCTGACAGC CCTAAGCCTG GGGCTCCACA CACCACGGCC 
CACATTGGAG CAGCCTCTTC AGGCTTCTCC CTTGAGCACC CCACGATGGT ACCTCACACT 
GAGGAGGGGC CAGTCCCCCT AGGACCAGAC CTGCTGACTG TGAGGAAGTC TGGTGTCAGC 
CGGACACACT CTCTGCCCAA TGACAGCTAC ATGTGCCGCA ATGGGAGCAC TGCCGAGAGA 
TCCCTAGGAC ACAGGGGCTG GGGGCTCCCC AAAGCCCAGT CAGGCTCCAT CTTGTCTGTT 
CACTCCCAAC CAGCAGACAC CAGCTGCATC CTACAGCTTC CCAAAGATGC ACACTATCTG 
CTCCAGCCTC ATGGGGCTCC CACCTGGGGC GCCATCCCTA AACTACCCCC ACCTGGCCGC 
TCCCCTCTGG CTCAGAGGCC TCTCAGGCGC CAGGCAGCAA TAAGGACTGA CTCCCTGGAC 
GTGCAGGGCC TGGGTAGCCG GGAAGACCTG TTGTCAGAGG TGAGTGGGCC CTCCTGCCCT 
CTGACCCGCT CCTCATCCTT CTGGGGCGGG TCGAGCATCC AGGTGCAGCA GCGCTCCGGC 
AGCCAGAGCA AAGTCTCCAA GCACATCCGC CTGCCAGCCC CTTGCCCAGG CCTGGAACCC 
AGCTGGGCCA AGGACCCTCA AGAGACCAGA AGCAGCTTAG AGCTGGACAC GGAGCTGAGC 
TGGATTTCAG GAGACCTCCT GCCCAGCAGT CAGGAAGAAC CCCTGTCCCC ACGGGACTTG 
AAAAAATGCT ACAGTGTAGA GGCCCAGAGC TGCCGGCGCA GGCCTGGGTC CTGGCTAGAC 
GAACAGAGGA GACACTCCAT CGCTGTCAGC TGCCTGGACA GCGGCTCCCA GCCCCGCCTA 
TGTCCAAGCC CCTCAAGCCT CGGGGGCCAA CCTCTTGGGG GCCCTGGGAG CCGGCCTAAG 
AAAAAACTCA GCCCACCCAG TATCTCTATA GACCCCCCGG AGAGCCAGGG CCCTCGGCCC 
CCATGCAGTC CTGGCGTCTG CCTCAGGAGG AGGGCGCCGG CCAGTGACTC GAAGGATCCC 
TCGGCCTCCA GCCCCCTTGA CAGCACGGCT GCCTCACCCT CCCCAAAGAA AGATGCGCTG 
AGTCTCTCTG GTTTGTCTTC TGACCCAACA GACCTGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increasing T-Type calcium channel activity by ?-adrenergic stimulation contributes to ?-adrenergic regulation of heart rates.
The Journal of physiology(2017 Dec)
Li Y,Zhang X,Zhang C,Zhang X,Li Y,Qi Z,Szeto C,Tang M,Peng Y,Molkentin JD,Houser SR,Xie M,Chen X

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Cacna1g cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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