APEX1 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	APEX1
Entrez Gene ID	11792
Full Name	apurinic/apyrimidinic endonuclease 1
Synonyms	APE,Apex,HAP1,Ref-1
General protein information	Preferred Names apurinic/apyrimidinic endonuclease 1 Names DNA-(apurinic or apyrimidinic site) lyase AP endonuclease 1 APEN APEX nuclease redox factor-1
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 14 Map Location: 14 C1\|14 26.3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009687.2	NP_033817.1	DNA-(apurinic or apyrimidinic site) lyase
XM_006518456.2	XP_006518519.1	DNA-(apurinic or apyrimidinic site) lyase isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	APEX1	NP_001138591.1
Mus musculus (house mouse)	Apex1	NP_033817.1
Gallus gallus (chicken)	APEX1	NP_001171688.1
Arabidopsis thaliana (thale cress)	ARP	NP_181677.1
Pan troglodytes (chimpanzee)	APEX1	NP_001074954.1
	APEX1	XP_001090240.1
Rattus norvegicus (Norway rat)	Apex1	NP_077062.1
Oryza sativa (rice)	Os01g0801000	NP_001044537.1
Bos taurus (cattle)	APEX1	NP_788782.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009587	XP_318609.4
Xenopus tropicalis (tropical clawed frog)	apex1	NP_989307.1
Homo sapiens (human)	APEX1	NP_001231178.1
Danio rerio (zebrafish)	apex1	NP_998586.1

Gene Ontology
Bibliography

Related articles in PubMed

Elevation of Serum APE1/Ref-1 in Experimental Murine Myocarditis.
Jin SA, Lim BK, Seo HJ, Kim SK, Ahn KT, Jeon BH, Jeong JO
International journal of molecular sciences18(12)(2017 Dec)

Mitochondrial DNA Integrity Is Maintained by APE1 in Carcinogen-Induced Colorectal Cancer.
Ballista-Hern?ndez J, Mart?nez-Ferrer M, V?lez R, Climent C, S?nchez-V?zquez MM, Torres C, Rodr?guez-Mu?oz A, Ayala-Pe?a S, Torres-Ramos CA
Molecular cancer research : MCR15(7)831-841(2017 Jul)

Nuclear complex of glyceraldehyde-3-phosphate dehydrogenase and DNA repair enzyme apurinic/apyrimidinic endonuclease I protect smooth muscle cells against oxidant-induced cell death.
Hou X, Snarski P, Higashi Y, Yoshida T, Jurkevich A, Delafontaine P, Sukhanov S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(7)3179-3192(2017 Jul)

Apurinic/Apyrimidinic Endonuclease 1/Redox Factor-1 (Ape1/Ref-1) Modulates Antigen Presenting Cell-mediated T Helper Cell Type 1 Responses.
Akhter N, Takeda Y, Nara H, Araki A, Ishii N, Asao N, Asao H
The Journal of biological chemistry291(45)23672-23680(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Suppression of Ape1/Ref-1 redox function leads to an increased cell surface retention of IL-12 and enhances Th1 responses.
Title: Apurinic/Apyrimidinic Endonuclease 1/Redox Factor-1 (Ape1/Ref-1) Modulates Antigen Presenting Cell-mediated T Helper Cell Type 1 Responses.

findings provide evidence that endogenous APE1 protects against ischemic infarction in both gray and white matter and facilitates the functional recovery of the central nervous system after mild stroke injury
Title: APE1/Ref-1 facilitates recovery of gray and white matter and neurological function after mild stroke injury.

Is closely associated with upregulation of the Ref1/Nrf2 signalling pathway.
Title: Acute exercise stress promotes Ref1/Nrf2 signalling and increases mitochondrial antioxidant activity in skeletal muscle.

increases in APEX1 level confer protection against the murine paternal age effect, thus highlighting the role of APEX1 in preserving reproductive health with increasing age and in protection against genotoxin-induced mutagenesis in somatic cells
Title: Increased human AP endonuclease 1 level confers protection against the paternal age effect in mice.

Endothelial cell tumor proliferation was found to be dependent on Apex-1 expression.
Title: Endothelial cell tumor growth is Ape/ref-1 dependent.

The following APEX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APEX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22808	NM_009687.2 Latest version!	Mus musculus apurinic/apyrimidinic endonuclease 1 (Apex1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu22808	XM_006518456.2 Latest version!	Mus musculus apurinic/apyrimidinic endonuclease 1 (Apex1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APEX1 ( NM_009687.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_033817.1 Mus musculus apurinic/apyrimidinic endonuclease 1 (Apex1), mRNA.

Price & Availability↑

CloneID	OMu22808
Clone ID Related Accession (Same CDS sequence)	NM_009687.2 , XM_006518456.2
Accession Version	NM_009687.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	DNA-(apurinic or apyrimidinic site) lyase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC027184.15 and BC052401.1. On Mar 19, 2011 this sequence version replaced NM_009687.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052401.1, AK013296.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCCAAAGC GGGGAAAGAA AGCGGCGGCC GACGACGGGG AAGAACCCAA GTCGGAGCCA 
GAGACCAAGA AGAGTAAGGG GGCAGCAAAG AAAACCGAGA AGGAGGCCGC GGGAGAGGGC 
CCTGTCCTGT ACGAGGACCC TCCAGATCAG AAAACCTCAC CCAGTGGCAA ATCTGCCACA 
CTCAAGATAT GCTCCTGGAA TGTGGATGGG CTTCGAGCCT GGATTAAAAA GAAAGGTTTG 
GATTGGGTAA AGGAAGAAGC ACCAGATATC TTGTGCCTCC AAGAGACCAA GTGCTCGGAG 
AACAAACTCC CGGCTGAACT GCAAGAGCTG CCTGGACTCA CCCATCAGTA CTGGTCAGCT 
CCGTCAGACA AAGAAGGATA CAGTGGTGTG GGCCTACTTT CCCGCCAGTG CCCGCTAAAA 
GTCTCTTATG GCATTGGCGA GGAAGAACAT GATCAAGAAG GCCGGGTGAT TGTGGCTGAA 
TTTGAGTCCT TTGTCCTGGT AACAGCCTAT GTTCCCAATG CAGGCAGGGG TCTGGTAAGA 
CTGGAATACC GACAGCGTTG GGATGAAGCC TTCCGAAAGT TTCTAAAGGA CTTGGCTTCC 
AGAAAGCCTC TTGTGCTATG TGGGGATCTC AATGTGGCTC ATGAAGAAAT TGACCTCCGT 
AACCCCAAAG GAAACAAAAA GAATGCTGGC TTTACTCCCC AGGAGCGCCA AGGTTTTGGG 
GAACTGCTAC AAGCTGTACC ATTGGCTGAC AGCTTCCGGC ATCTCTACCC CAACACTGCT 
TACGCTTACA CTTTCTGGAC TTACATGATG AATGCCCGCT CTAAGAATGT TGGTTGGCGC 
CTTGATTACT TTTTGCTTTC CCACTCTCTT TTACCTGCAT TGTGTGACAG CAAGATCCGG 
TCCAAGGCTC TTGGCAGTGA CCACTGTCCC ATCACCCTTT ACCTAGCACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033817.1
CDS	139..1092
Translation	MPKRGKKAAADDGEEPKSEPETKKSKGAAKKTEKEAAGEGPVLYEDPPDQKTSPSGKSATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAELQELPGLTHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGEEEHDQEGRVIVAEFESFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKDLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTAYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL

Target ORF information:

RefSeq Version	NM_009687.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus apurinic/apyrimidinic endonuclease 1 (Apex1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009687.2

ORF Insert Sequence:

ATGCCAAAGC GGGGAAAGAA AGCGGCGGCC GACGACGGGG AAGAACCCAA GTCGGAGCCA 
GAGACCAAGA AGAGTAAGGG GGCAGCAAAG AAAACCGAGA AGGAGGCCGC GGGAGAGGGC 
CCTGTCCTGT ACGAGGACCC TCCAGATCAG AAAACCTCAC CCAGTGGCAA ATCTGCCACA 
CTCAAGATAT GCTCCTGGAA TGTGGATGGG CTTCGAGCCT GGATTAAAAA GAAAGGTTTG 
GATTGGGTAA AGGAAGAAGC ACCAGATATC TTGTGCCTCC AAGAGACCAA GTGCTCGGAG 
AACAAACTCC CGGCTGAACT GCAAGAGCTG CCTGGACTCA CCCATCAGTA CTGGTCAGCT 
CCGTCAGACA AAGAAGGATA CAGTGGTGTG GGCCTACTTT CCCGCCAGTG CCCGCTAAAA 
GTCTCTTATG GCATTGGCGA GGAAGAACAT GATCAAGAAG GCCGGGTGAT TGTGGCTGAA 
TTTGAGTCCT TTGTCCTGGT AACAGCCTAT GTTCCCAATG CAGGCAGGGG TCTGGTAAGA 
CTGGAATACC GACAGCGTTG GGATGAAGCC TTCCGAAAGT TTCTAAAGGA CTTGGCTTCC 
AGAAAGCCTC TTGTGCTATG TGGGGATCTC AATGTGGCTC ATGAAGAAAT TGACCTCCGT 
AACCCCAAAG GAAACAAAAA GAATGCTGGC TTTACTCCCC AGGAGCGCCA AGGTTTTGGG 
GAACTGCTAC AAGCTGTACC ATTGGCTGAC AGCTTCCGGC ATCTCTACCC CAACACTGCT 
TACGCTTACA CTTTCTGGAC TTACATGATG AATGCCCGCT CTAAGAATGT TGGTTGGCGC 
CTTGATTACT TTTTGCTTTC CCACTCTCTT TTACCTGCAT TGTGTGACAG CAAGATCCGG 
TCCAAGGCTC TTGGCAGTGA CCACTGTCCC ATCACCCTTT ACCTAGCACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APEX1 ( XM_006518456.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006518519.1 Mus musculus apurinic/apyrimidinic endonuclease 1 (Apex1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu22808
Clone ID Related Accession (Same CDS sequence)	NM_009687.2 , XM_006518456.2
Accession Version	XM_006518456.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	DNA-(apurinic or apyrimidinic site) lyase isoform X1
Comment

ATGCCAAAGC GGGGAAAGAA AGCGGCGGCC GACGACGGGG AAGAACCCAA GTCGGAGCCA 
GAGACCAAGA AGAGTAAGGG GGCAGCAAAG AAAACCGAGA AGGAGGCCGC GGGAGAGGGC 
CCTGTCCTGT ACGAGGACCC TCCAGATCAG AAAACCTCAC CCAGTGGCAA ATCTGCCACA 
CTCAAGATAT GCTCCTGGAA TGTGGATGGG CTTCGAGCCT GGATTAAAAA GAAAGGTTTG 
GATTGGGTAA AGGAAGAAGC ACCAGATATC TTGTGCCTCC AAGAGACCAA GTGCTCGGAG 
AACAAACTCC CGGCTGAACT GCAAGAGCTG CCTGGACTCA CCCATCAGTA CTGGTCAGCT 
CCGTCAGACA AAGAAGGATA CAGTGGTGTG GGCCTACTTT CCCGCCAGTG CCCGCTAAAA 
GTCTCTTATG GCATTGGCGA GGAAGAACAT GATCAAGAAG GCCGGGTGAT TGTGGCTGAA 
TTTGAGTCCT TTGTCCTGGT AACAGCCTAT GTTCCCAATG CAGGCAGGGG TCTGGTAAGA 
CTGGAATACC GACAGCGTTG GGATGAAGCC TTCCGAAAGT TTCTAAAGGA CTTGGCTTCC 
AGAAAGCCTC TTGTGCTATG TGGGGATCTC AATGTGGCTC ATGAAGAAAT TGACCTCCGT 
AACCCCAAAG GAAACAAAAA GAATGCTGGC TTTACTCCCC AGGAGCGCCA AGGTTTTGGG 
GAACTGCTAC AAGCTGTACC ATTGGCTGAC AGCTTCCGGC ATCTCTACCC CAACACTGCT 
TACGCTTACA CTTTCTGGAC TTACATGATG AATGCCCGCT CTAAGAATGT TGGTTGGCGC 
CTTGATTACT TTTTGCTTTC CCACTCTCTT TTACCTGCAT TGTGTGACAG CAAGATCCGG 
TCCAAGGCTC TTGGCAGTGA CCACTGTCCC ATCACCCTTT ACCTAGCACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518519.1
Translation	MPKRGKKAAADDGEEPKSEPETKKSKGAAKKTEKEAAGEGPVLYEDPPDQKTSPSGKSATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAELQELPGLTHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGEEEHDQEGRVIVAEFESFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKDLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTAYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL

Target ORF information:

RefSeq Version	XM_006518456.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus apurinic/apyrimidinic endonuclease 1 (Apex1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518456.2

ORF Insert Sequence:

ATGCCAAAGC GGGGAAAGAA AGCGGCGGCC GACGACGGGG AAGAACCCAA GTCGGAGCCA 
GAGACCAAGA AGAGTAAGGG GGCAGCAAAG AAAACCGAGA AGGAGGCCGC GGGAGAGGGC 
CCTGTCCTGT ACGAGGACCC TCCAGATCAG AAAACCTCAC CCAGTGGCAA ATCTGCCACA 
CTCAAGATAT GCTCCTGGAA TGTGGATGGG CTTCGAGCCT GGATTAAAAA GAAAGGTTTG 
GATTGGGTAA AGGAAGAAGC ACCAGATATC TTGTGCCTCC AAGAGACCAA GTGCTCGGAG 
AACAAACTCC CGGCTGAACT GCAAGAGCTG CCTGGACTCA CCCATCAGTA CTGGTCAGCT 
CCGTCAGACA AAGAAGGATA CAGTGGTGTG GGCCTACTTT CCCGCCAGTG CCCGCTAAAA 
GTCTCTTATG GCATTGGCGA GGAAGAACAT GATCAAGAAG GCCGGGTGAT TGTGGCTGAA 
TTTGAGTCCT TTGTCCTGGT AACAGCCTAT GTTCCCAATG CAGGCAGGGG TCTGGTAAGA 
CTGGAATACC GACAGCGTTG GGATGAAGCC TTCCGAAAGT TTCTAAAGGA CTTGGCTTCC 
AGAAAGCCTC TTGTGCTATG TGGGGATCTC AATGTGGCTC ATGAAGAAAT TGACCTCCGT 
AACCCCAAAG GAAACAAAAA GAATGCTGGC TTTACTCCCC AGGAGCGCCA AGGTTTTGGG 
GAACTGCTAC AAGCTGTACC ATTGGCTGAC AGCTTCCGGC ATCTCTACCC CAACACTGCT 
TACGCTTACA CTTTCTGGAC TTACATGATG AATGCCCGCT CTAAGAATGT TGGTTGGCGC 
CTTGATTACT TTTTGCTTTC CCACTCTCTT TTACCTGCAT TGTGTGACAG CAAGATCCGG 
TCCAAGGCTC TTGGCAGTGA CCACTGTCCC ATCACCCTTT ACCTAGCACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Elevation of Serum APE1/Ref-1 in Experimental Murine Myocarditis.
International journal of molecular sciences18(12)(2017 Dec)
Jin SA,Lim BK,Seo HJ,Kim SK,Ahn KT,Jeon BH,Jeong JO

Mitochondrial DNA Integrity Is Maintained by APE1 in Carcinogen-Induced Colorectal Cancer.
Molecular cancer research : MCR15(7)831-841(2017 Jul)
Ballista-Hern?ndez J,Mart?nez-Ferrer M,V?lez R,Climent C,S?nchez-V?zquez MM,Torres C,Rodr?guez-Mu?oz A,Ayala-Pe?a S,Torres-Ramos CA

Nuclear complex of glyceraldehyde-3-phosphate dehydrogenase and DNA repair enzyme apurinic/apyrimidinic endonuclease I protect smooth muscle cells against oxidant-induced cell death.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(7)3179-3192(2017 Jul)
Hou X,Snarski P,Higashi Y,Yoshida T,Jurkevich A,Delafontaine P,Sukhanov S

Apurinic/Apyrimidinic Endonuclease 1/Redox Factor-1 (Ape1/Ref-1) Modulates Antigen Presenting Cell-mediated T Helper Cell Type 1 Responses.
The Journal of biological chemistry291(45)23672-23680(2016 Nov)
Akhter N,Takeda Y,Nara H,Araki A,Ishii N,Asao N,Asao H

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APEX1 cDNA ORF clone, Mus musculus(House mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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