Actl7a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Actl7a
Entrez Gene ID	11470
Full Name	actin-like 7a
Synonyms	Tact2
General protein information	Preferred Names actin-like 7a Names actin-like protein 7A T-actin-2 actin-like Tact2 actin-like-7-alpha t-actin 2 testis-specific actin-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 B3\|4 31.66 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009611.3	NP_033741.1	actin-like protein 7A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ACTL7A	NP_001233289.1
Mus musculus (house mouse)	Actl7a	NP_033741.1
Rattus norvegicus (Norway rat)	Actl7a	NP_001011973.1
Canis lupus familiaris (dog)	ACTL7A	XP_005626958.1
Bos taurus (cattle)	ACTL7A	NP_001033659.1
Homo sapiens (human)	ACTL7A	NP_006678.1
	ACTL7A	XP_001108489.1

Gene Ontology
Bibliography

Related articles in PubMed

Dynamic alterations in the expression and localization of ACTL7a during capacitation in mouse spermatozoa.
Fu J, Song W, Zong S, Koide SS, Miao S, Wang L
Fertility and sterility99(3)882-8(2013 Mar)

Anti-ACTL7a antibodies: a cause of infertility.
Fu J, Wang Y, Fok KL, Yang D, Qiu Y, Chan HC, Koide SS, Miao S, Wang L
Fertility and sterility97(5)1226-33.e1-8(2012 May)

Molecular recognition of the Tes LIM2-3 domains by the actin-related protein Arp7A.
Bo?da B, Knowles PP, Briggs DC, Murray-Rust J, Soriano E, Garvalov BK, McDonald NQ, Way M
The Journal of biological chemistry286(13)11543-54(2011 Apr)

Novel actin-like proteins T-ACTIN 1 and T-ACTIN 2 are differentially expressed in the cytoplasm and nucleus of mouse haploid germ cells.
Tanaka H, Iguchi N, Egydio de Carvalho C, Tadokoro Y, Yomogida K, Nishimune Y
Biology of reproduction69(2)475-82(2003 Aug)

Genomic structure and promoter activity of the testis haploid germ cell-specific intronless genes, Tact1 and Tact2.
Hisano M, Yamada S, Tanaka H, Nishimune Y, Nozaki M
Molecular reproduction and development65(2)148-56(2003 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ACTL7a is an essential component of capacitation in mouse spermatozoa.
Title: Dynamic alterations in the expression and localization of ACTL7a during capacitation in mouse spermatozoa.

Anti-ACTL7a antibodies may cause infertility in mice because the in vitro treatment of mouse spermatozoa with ACTL7a antibody-containing serum markedly reduced the fertilizing potential of the spermatozoa.
Title: Anti-ACTL7a antibodies: a cause of infertility.

results suggest that T-ACTINs play important roles in sperm function and in the specific morphogenesis of spermatozoa during spermiogenesis
Title: Novel actin-like proteins T-ACTIN 1 and T-ACTIN 2 are differentially expressed in the cytoplasm and nucleus of mouse haploid germ cells.

The following Actl7a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Actl7a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58424	NM_009611.3 Latest version!	Mus musculus actin-like 7a (Actl7a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Actl7a ( NM_009611.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033741.1 Mus musculus actin-like 7a (Actl7a), mRNA.

Price & Availability↑

CloneID	OMu58424
Clone ID Related Accession (Same CDS sequence)	NM_009611.3
Accession Version	NM_009611.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	actin-like protein 7A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB023063.1 and BC049610.1. On Oct 31, 2007 this sequence version replaced NM_009611.2. ##Evidence-Data-START## Transcript is intronless :: AB023063.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTGG ATGGTGTGTG GGCTCCACAG ACAGCAAACA TTGGGGACGG ACCTGCCAAG 
AAAGCTAGTG ACCAGGCCTC CATGCAGACA CAGGTCCTCC AAACCGCCTC CCTAAAAGAT 
GGCCCAGCAA AAAGGGCGGT ATGGGTCCGT CGGGACAATG CTGAGACAGA AGACCCTGTT 
AAGTCAACTA TGTCCAAGGA TAGGCCAAGA CTAGAGGTAA CCAAAGCAGT GGTTGTGGAC 
CTGGGCACTG GCTTCTGTAA ATGTGGCTTT GCTGGCCTGC CAAAGCCCAC TCATAAGATC 
TCAACAACAG TGGGCAAGCC CTACATGGAG ACGGCCAAGA CGGGGGATAA TCGCAAAGAG 
ACCTTTGTGG GGCACGAGCT CTTTAACCCA GATATACATC TCAAGCTGGT CAACCCCCTG 
CGCCATGGCA TCATTGTGGA CTGGGACACA GTACAGGACA TCTGGGAATA CCTCTTCCGA 
CAGGAGATGA AGATCGCCCC AGAGGAACAC GCGGTCCTGG TTTCAGACCC ACCCCTGAGT 
CCTCATACCA ACAGAGAGAA GTACGCCGAG ATGCTGTTTG AGACCTTCAA CACACCTGCA 
ATGCACATCG CCTACCAATC CCGCCTGTCC ATGTACTCCT ACGGACGCAC CTCTGGCCTG 
GTGGTGGAGG TTGGACATGG TGTGTCCTAT GTGGTCCCCA TCTATGAGGG TTATCCTTTG 
CCCAGCATCA CGGGGAGGCT AGACTACGCA GGTTCTGACC TAACGACCTA CCTGATGAAC 
CTGATGAACA ACTCTGGAAA ACACTTCTCT GAGGACCATC TCGGTATTGT GGAGGACATC 
AAGACGAGGT GCTGCTTCGT GGCCCTGGAC CCCATTGAAG AGAAGAAAAT CCCTGCCCCT 
GAACATGAGA TCCATTATAC CCTGCCTGAT GGGAAAGAGA TCCGTCTGGG TCAGGAGAGG 
TTCCTCTGCT CGGAGATGTT CTTCAAGCCA TCTCTAATCA AGTCCATGCA GCTGGGCCTC 
CACACCCAGA CAGTGTCCTG CCTTAACAAA TGTGACATAG CACTCAAACG AGACCTCATG 
GGGAACATCC TGCTCTGTGG GGGCAGCACT ATGCTCAGGG GTTTCCCTAA CCGTCTGCAG 
AAGGAGCTGA GCAGCATGTG CCCCAACGAC ACCCCCCAGG TCAATGTGCT GCCCGAGAGA 
GACACTGCAG TCTGGACTGG TGGGTCTATC CTGGCCTCAC TCCAGGGCTT TCAACCACTG 
TGGGTCCATC GCCTGGAGTA CGAGGAACAT GGGCCTTTCT TCCTCTACAG AAGGTGCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033741.1
CDS	54..1376
Translation	MSLDGVWAPQTANIGDGPAKKASDQASMQTQVLQTASLKDGPAKRAVWVRRDNAETEDPVKSTMSKDRPRLEVTKAVVVDLGTGFCKCGFAGLPKPTHKISTTVGKPYMETAKTGDNRKETFVGHELFNPDIHLKLVNPLRHGIIVDWDTVQDIWEYLFRQEMKIAPEEHAVLVSDPPLSPHTNREKYAEMLFETFNTPAMHIAYQSRLSMYSYGRTSGLVVEVGHGVSYVVPIYEGYPLPSITGRLDYAGSDLTTYLMNLMNNSGKHFSEDHLGIVEDIKTRCCFVALDPIEEKKIPAPEHEIHYTLPDGKEIRLGQERFLCSEMFFKPSLIKSMQLGLHTQTVSCLNKCDIALKRDLMGNILLCGGSTMLRGFPNRLQKELSSMCPNDTPQVNVLPERDTAVWTGGSILASLQGFQPLWVHRLEYEEHGPFFLYRRCF

Target ORF information:

RefSeq Version	NM_009611.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus actin-like 7a (Actl7a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009611.3

ORF Insert Sequence:

ATGTCTCTGG ATGGTGTGTG GGCTCCACAG ACAGCAAACA TTGGGGACGG ACCTGCCAAG 
AAAGCTAGTG ACCAGGCCTC CATGCAGACA CAGGTCCTCC AAACCGCCTC CCTAAAAGAT 
GGCCCAGCAA AAAGGGCGGT ATGGGTCCGT CGGGACAATG CTGAGACAGA AGACCCTGTT 
AAGTCAACTA TGTCCAAGGA TAGGCCAAGA CTAGAGGTAA CCAAAGCAGT GGTTGTGGAC 
CTGGGCACTG GCTTCTGTAA ATGTGGCTTT GCTGGCCTGC CAAAGCCCAC TCATAAGATC 
TCAACAACAG TGGGCAAGCC CTACATGGAG ACGGCCAAGA CGGGGGATAA TCGCAAAGAG 
ACCTTTGTGG GGCACGAGCT CTTTAACCCA GATATACATC TCAAGCTGGT CAACCCCCTG 
CGCCATGGCA TCATTGTGGA CTGGGACACA GTACAGGACA TCTGGGAATA CCTCTTCCGA 
CAGGAGATGA AGATCGCCCC AGAGGAACAC GCGGTCCTGG TTTCAGACCC ACCCCTGAGT 
CCTCATACCA ACAGAGAGAA GTACGCCGAG ATGCTGTTTG AGACCTTCAA CACACCTGCA 
ATGCACATCG CCTACCAATC CCGCCTGTCC ATGTACTCCT ACGGACGCAC CTCTGGCCTG 
GTGGTGGAGG TTGGACATGG TGTGTCCTAT GTGGTCCCCA TCTATGAGGG TTATCCTTTG 
CCCAGCATCA CGGGGAGGCT AGACTACGCA GGTTCTGACC TAACGACCTA CCTGATGAAC 
CTGATGAACA ACTCTGGAAA ACACTTCTCT GAGGACCATC TCGGTATTGT GGAGGACATC 
AAGACGAGGT GCTGCTTCGT GGCCCTGGAC CCCATTGAAG AGAAGAAAAT CCCTGCCCCT 
GAACATGAGA TCCATTATAC CCTGCCTGAT GGGAAAGAGA TCCGTCTGGG TCAGGAGAGG 
TTCCTCTGCT CGGAGATGTT CTTCAAGCCA TCTCTAATCA AGTCCATGCA GCTGGGCCTC 
CACACCCAGA CAGTGTCCTG CCTTAACAAA TGTGACATAG CACTCAAACG AGACCTCATG 
GGGAACATCC TGCTCTGTGG GGGCAGCACT ATGCTCAGGG GTTTCCCTAA CCGTCTGCAG 
AAGGAGCTGA GCAGCATGTG CCCCAACGAC ACCCCCCAGG TCAATGTGCT GCCCGAGAGA 
GACACTGCAG TCTGGACTGG TGGGTCTATC CTGGCCTCAC TCCAGGGCTT TCAACCACTG 
TGGGTCCATC GCCTGGAGTA CGAGGAACAT GGGCCTTTCT TCCTCTACAG AAGGTGCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynamic alterations in the expression and localization of ACTL7a during capacitation in mouse spermatozoa.
Fertility and sterility99(3)882-8(2013 Mar)
Fu J,Song W,Zong S,Koide SS,Miao S,Wang L

Anti-ACTL7a antibodies: a cause of infertility.
Fertility and sterility97(5)1226-33.e1-8(2012 May)
Fu J,Wang Y,Fok KL,Yang D,Qiu Y,Chan HC,Koide SS,Miao S,Wang L

Molecular recognition of the Tes LIM2-3 domains by the actin-related protein Arp7A.
The Journal of biological chemistry286(13)11543-54(2011 Apr)
Bo?da B,Knowles PP,Briggs DC,Murray-Rust J,Soriano E,Garvalov BK,McDonald NQ,Way M

Novel actin-like proteins T-ACTIN 1 and T-ACTIN 2 are differentially expressed in the cytoplasm and nucleus of mouse haploid germ cells.
Biology of reproduction69(2)475-82(2003 Aug)
Tanaka H,Iguchi N,Egydio de Carvalho C,Tadokoro Y,Yomogida K,Nishimune Y

Genomic structure and promoter activity of the testis haploid germ cell-specific intronless genes, Tact1 and Tact2.
Molecular reproduction and development65(2)148-56(2003 Jun)
Hisano M,Yamada S,Tanaka H,Nishimune Y,Nozaki M