Prn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prn
Entrez Gene ID	111368
Full Name	prion protein readthrough transcript
Synonyms	Gm28045,Gm44637,Prnp-Prnd
General protein information	Preferred Names prion protein readthrough transcript Names prion protein gene complex Prnp-Prnd readthrough
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 F2\|2
Summary	This locus represents naturally occurring readthrough transcription between the neighboring Prnp (major prion protein) and Prnd (prion-like protein doppel) genes on chromosome 2. The readthrough transcript encodes a protein that shares sequence identity with the downstream gene product but its N-terminal region is distinct due to the presence of an in-frame, upstream AUG compared to the downstream gene. [provided by RefSeq, May 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001278258.1	NP_001265187.1	prion protein gene complex
NM_001278259.1	NP_001265188.1	prion protein gene complex

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PRND	XP_525256.1
Canis lupus familiaris (dog)	PRND	XP_005634877.1
Bos taurus (cattle)	PRND	NP_776583.1
Rattus norvegicus (Norway rat)	Prnd	NP_001095901.1
Homo sapiens (human)	PRND	NP_036541.2
	PRND	XP_001115569.1
Mus musculus (house mouse)	Prn	NP_001265187.1
Mus musculus (house mouse)	Prnd	NP_001119810.1

Gene Ontology
Bibliography

Related articles in PubMed

The N Terminus of the Prion Protein Mediates Functional Interactions with the Neuronal Cell Adhesion Molecule (NCAM) Fibronectin Domain.
Slap?ak U, Salzano G, Amin L, Abskharon RN, Ilc G, Zupan?i? B, Biljan I, Plavec J, Giachin G, Legname G
The Journal of biological chemistry291(42)21857-21868(2016 Oct)

Preferential Cu2+ coordination by His96 and His111 induces beta-sheet formation in the unstructured amyloidogenic region of the prion protein.
Jones CE, Abdelraheim SR, Brown DR, Viles JH
The Journal of biological chemistry279(31)32018-27(2004 Jul)

Prion proteins and ECTO-NOX proteins exhibit similar oscillating redox activities.
Kim C, Morr? DJ
Biochemical and biophysical research communications315(4)1140-6(2004 Mar)

Identification of a novel gene encoding a PrP-like protein expressed as chimeric transcripts fused to PrP exon 1/2 in ataxic mouse line with a disrupted PrP gene.
Li A, Sakaguchi S, Atarashi R, Roy BC, Nakaoke R, Arima K, Okimura N, Kopacek J, Shigematsu K
Cellular and molecular neurobiology20(5)553-67(2000 Oct)

Characterisation of two promoters for prion protein (PrP) gene expression in neuronal cells.
Baybutt H, Manson J
Gene184(1)125-31(1997 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Preferential Cu2+ coordination by His96 and His111 induces beta-sheet formation in the unstructured amyloidogenic region of the prion protein
Title: Preferential Cu2+ coordination by His96 and His111 induces beta-sheet formation in the unstructured amyloidogenic region of the prion protein.

The following Prn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16813	NM_001278259.1 Latest version!	Mus musculus prion protein readthrough transcript (Prn), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu16813	NM_001278258.1 Latest version!	Mus musculus prion protein readthrough transcript (Prn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prn ( NM_001278258.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001265187.1 Mus musculus prion protein readthrough transcript (Prn), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16813
Clone ID Related Accession (Same CDS sequence)	NM_001278258.1 , NM_001278259.1
Accession Version	NM_001278258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	prion protein gene complex
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL833794.9. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript was derived from the reference genome assembly. The genomic coordinates were determined from alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## readthrough transcript :: includes exons from GeneID 19122, 26434 ##RefSeq-Attributes-END##

ATGATGGAGT GGAGGTCGCT TCGAGTGGAG GTCGCTTCGC GCACCGGATT CACCATGAAG 
AACCGGCTGG GTACATGGTG GGTGGCCATC CTCTGCATGC TGCTTGCCAG CCACCTCTCC 
ACGGTCAAGG CAAGGGGCAT AAAGCACAGG TTCAAGTGGA ACCGGAAGGT CCTGCCCAGC 
AGCGGCGGCC AGATCACCGA AGCTCGGGTA GCTGAGAACC GCCCAGGAGC CTTCATCAAG 
CAAGGCCGGA AGCTGGACAT CGACTTTGGA GCAGAGGGCA ACAGGTACTA CGCGGCTAAC 
TATTGGCAGT TCCCTGATGG GATCTACTAC GAAGGCTGCT CTGAAGCCAA CGTGACCAAG 
GAGATGCTGG TGACCAGCTG CGTCAACGCC ACCCAGGCGG CCAACCAGGC TGAGTTCTCC 
CGGGAGAAGC AGGATAGCAA GCTCCACCAG CGAGTCCTGT GGCGGCTGAT CAAAGAGATC 
TGCTCCGCCA AGCACTGCGA TTTCTGGCTG GAAAGGGGAG CTGCGCTTCG GGTCGCCGTG 
GACCAACCGG CGATGGTCTG CCTGCTGGGT TTCGTTTGGT TCATTGTGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265187.1
CDS	231..824
Translation	MMEWRSLRVEVASRTGFTMKNRLGTWWVAILCMLLASHLSTVKARGIKHRFKWNRKVLPSSGGQITEARVAENRPGAFIKQGRKLDIDFGAEGNRYYAANYWQFPDGIYYEGCSEANVTKEMLVTSCVNATQAANQAEFSREKQDSKLHQRVLWRLIKEICSAKHCDFWLERGAALRVAVDQPAMVCLLGFVWFIVK

Target ORF information:

RefSeq Version	NM_001278258.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prion protein readthrough transcript (Prn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278258.1

ORF Insert Sequence:

ATGATGGAGT GGAGGTCGCT TCGAGTGGAG GTCGCTTCGC GCACCGGATT CACCATGAAG 
AACCGGCTGG GTACATGGTG GGTGGCCATC CTCTGCATGC TGCTTGCCAG CCACCTCTCC 
ACGGTCAAGG CAAGGGGCAT AAAGCACAGG TTCAAGTGGA ACCGGAAGGT CCTGCCCAGC 
AGCGGCGGCC AGATCACCGA AGCTCGGGTA GCTGAGAACC GCCCAGGAGC CTTCATCAAG 
CAAGGCCGGA AGCTGGACAT CGACTTTGGA GCAGAGGGCA ACAGGTACTA CGCGGCTAAC 
TATTGGCAGT TCCCTGATGG GATCTACTAC GAAGGCTGCT CTGAAGCCAA CGTGACCAAG 
GAGATGCTGG TGACCAGCTG CGTCAACGCC ACCCAGGCGG CCAACCAGGC TGAGTTCTCC 
CGGGAGAAGC AGGATAGCAA GCTCCACCAG CGAGTCCTGT GGCGGCTGAT CAAAGAGATC 
TGCTCCGCCA AGCACTGCGA TTTCTGGCTG GAAAGGGGAG CTGCGCTTCG GGTCGCCGTG 
GACCAACCGG CGATGGTCTG CCTGCTGGGT TTCGTTTGGT TCATTGTGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prn ( NM_001278259.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001265188.1 Mus musculus prion protein readthrough transcript (Prn), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16813
Clone ID Related Accession (Same CDS sequence)	NM_001278258.1 , NM_001278259.1
Accession Version	NM_001278259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	prion protein gene complex
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL833794.9. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript was derived from the reference genome assembly. The genomic coordinates were determined from alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## readthrough transcript :: includes exons from GeneID 19122, 26434 ##RefSeq-Attributes-END##

ATGATGGAGT GGAGGTCGCT TCGAGTGGAG GTCGCTTCGC GCACCGGATT CACCATGAAG 
AACCGGCTGG GTACATGGTG GGTGGCCATC CTCTGCATGC TGCTTGCCAG CCACCTCTCC 
ACGGTCAAGG CAAGGGGCAT AAAGCACAGG TTCAAGTGGA ACCGGAAGGT CCTGCCCAGC 
AGCGGCGGCC AGATCACCGA AGCTCGGGTA GCTGAGAACC GCCCAGGAGC CTTCATCAAG 
CAAGGCCGGA AGCTGGACAT CGACTTTGGA GCAGAGGGCA ACAGGTACTA CGCGGCTAAC 
TATTGGCAGT TCCCTGATGG GATCTACTAC GAAGGCTGCT CTGAAGCCAA CGTGACCAAG 
GAGATGCTGG TGACCAGCTG CGTCAACGCC ACCCAGGCGG CCAACCAGGC TGAGTTCTCC 
CGGGAGAAGC AGGATAGCAA GCTCCACCAG CGAGTCCTGT GGCGGCTGAT CAAAGAGATC 
TGCTCCGCCA AGCACTGCGA TTTCTGGCTG GAAAGGGGAG CTGCGCTTCG GGTCGCCGTG 
GACCAACCGG CGATGGTCTG CCTGCTGGGT TTCGTTTGGT TCATTGTGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265188.1
CDS	231..824
Translation	MMEWRSLRVEVASRTGFTMKNRLGTWWVAILCMLLASHLSTVKARGIKHRFKWNRKVLPSSGGQITEARVAENRPGAFIKQGRKLDIDFGAEGNRYYAANYWQFPDGIYYEGCSEANVTKEMLVTSCVNATQAANQAEFSREKQDSKLHQRVLWRLIKEICSAKHCDFWLERGAALRVAVDQPAMVCLLGFVWFIVK

Target ORF information:

RefSeq Version	NM_001278259.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prion protein readthrough transcript (Prn), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278259.1

ORF Insert Sequence:

ATGATGGAGT GGAGGTCGCT TCGAGTGGAG GTCGCTTCGC GCACCGGATT CACCATGAAG 
AACCGGCTGG GTACATGGTG GGTGGCCATC CTCTGCATGC TGCTTGCCAG CCACCTCTCC 
ACGGTCAAGG CAAGGGGCAT AAAGCACAGG TTCAAGTGGA ACCGGAAGGT CCTGCCCAGC 
AGCGGCGGCC AGATCACCGA AGCTCGGGTA GCTGAGAACC GCCCAGGAGC CTTCATCAAG 
CAAGGCCGGA AGCTGGACAT CGACTTTGGA GCAGAGGGCA ACAGGTACTA CGCGGCTAAC 
TATTGGCAGT TCCCTGATGG GATCTACTAC GAAGGCTGCT CTGAAGCCAA CGTGACCAAG 
GAGATGCTGG TGACCAGCTG CGTCAACGCC ACCCAGGCGG CCAACCAGGC TGAGTTCTCC 
CGGGAGAAGC AGGATAGCAA GCTCCACCAG CGAGTCCTGT GGCGGCTGAT CAAAGAGATC 
TGCTCCGCCA AGCACTGCGA TTTCTGGCTG GAAAGGGGAG CTGCGCTTCG GGTCGCCGTG 
GACCAACCGG CGATGGTCTG CCTGCTGGGT TTCGTTTGGT TCATTGTGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The N Terminus of the Prion Protein Mediates Functional Interactions with the Neuronal Cell Adhesion Molecule (NCAM) Fibronectin Domain.
The Journal of biological chemistry291(42)21857-21868(2016 Oct)
Slap?ak U,Salzano G,Amin L,Abskharon RN,Ilc G,Zupan?i? B,Biljan I,Plavec J,Giachin G,Legname G

Preferential Cu2+ coordination by His96 and His111 induces beta-sheet formation in the unstructured amyloidogenic region of the prion protein.
The Journal of biological chemistry279(31)32018-27(2004 Jul)
Jones CE,Abdelraheim SR,Brown DR,Viles JH

Prion proteins and ECTO-NOX proteins exhibit similar oscillating redox activities.
Biochemical and biophysical research communications315(4)1140-6(2004 Mar)
Kim C,Morr? DJ

Identification of a novel gene encoding a PrP-like protein expressed as chimeric transcripts fused to PrP exon 1/2 in ataxic mouse line with a disrupted PrP gene.
Cellular and molecular neurobiology20(5)553-67(2000 Oct)
Li A,Sakaguchi S,Atarashi R,Roy BC,Nakaoke R,Arima K,Okimura N,Kopacek J,Shigematsu K

Characterisation of two promoters for prion protein (PrP) gene expression in neuronal cells.
Gene184(1)125-31(1997 Jan)
Baybutt H,Manson J