Dut cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dut
Entrez Gene ID	110074
Full Name	deoxyuridine triphosphatase
Synonyms	5031412I06Rik,5133400F09Rik,D2Bwg0749e,Dutp,dUTPase
General protein information	Preferred Names deoxyuridine triphosphatase Names deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 F1\|2 61.33 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001159646.1	NP_001153118.1	deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial isoform 1
NM_023595.6	NP_076084.2	deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial isoform 2

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	dut-1	NP_001021553.1
Bos taurus (cattle)	DUT	NP_001071341.1
Mus musculus (house mouse)	Dut	NP_001153118.1
Canis lupus familiaris (dog)	DUT	XP_851889.1
Danio rerio (zebrafish)	dut	NP_001006005.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010159	XP_319329.3
Eremothecium gossypii	AGOS_AGR249C	NP_986915.1
Pan troglodytes (chimpanzee)	DUT	XP_523069.2
Rattus norvegicus (Norway rat)	Dut	NP_446044.1
Drosophila melanogaster (fruit fly)	dUTPase	NP_609479.1
Xenopus tropicalis (tropical clawed frog)	LOC101733437	XP_004912658.1
Homo sapiens (human)	DUT	NP_001020419.1
Gallus gallus (chicken)	DUT	NP_001072956.1

Gene Ontology
Bibliography

Related articles in PubMed

RORalpha coordinates reciprocal signaling in cerebellar development through sonic hedgehog and calcium-dependent pathways.
Gold DA, Baek SH, Schork NJ, Rose DW, Larsen DD, Sachs BD, Rosenfeld MG, Hamilton BA
Neuron40(6)1119-31(2003 Dec)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

Novel gain of function activity of p53 mutants: activation of the dUTPase gene expression leading to resistance to 5-fluorouracil.
Pugacheva EN, Ivanov AV, Kravchenko JE, Kopnin BP, Levine AJ, Chumakov PM
Oncogene21(30)4595-600(2002 Jul)

Embryonic lethality and vascular defects in mice lacking the Notch ligand Jagged1.
Xue Y, Gao X, Lindsell CE, Norton CR, Chang B, Hicks C, Gendron-Maguire M, Rand EB, Weinmaster G, Gridley T
Human molecular genetics8(5)723-30(1999 May)

Cloning and expression of the mouse deoxyuridine triphosphate nucleotidohydrolase gene: differs from the rat enzyme in that it lacks nuclear receptor interacting LXXLL motif.
Kan L, Jain S, Cook W, Cao WQ, Usuda N, Yeldandi AV, Rao MS, Kanwar YS, Reddy JK
Gene expression8(4)231-46(1999)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Novel gain of function activity of p53 mutants: activation of the dUTPase gene expression leading to resistance to 5-fluorouracil.
Title: Novel gain of function activity of p53 mutants: activation of the dUTPase gene expression leading to resistance to 5-fluorouracil.

The following Dut gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dut cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu04556	NM_023595.6 Latest version!	Mus musculus deoxyuridine triphosphatase (Dut), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu07691	NM_001159646.1 Latest version!	Mus musculus deoxyuridine triphosphatase (Dut), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dut ( NM_023595.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_076084.2 Mus musculus deoxyuridine triphosphatase (Dut), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu04556
Clone ID Related Accession (Same CDS sequence)	NM_023595.6
Accession Version	NM_023595.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Mus musculus(house mouse)
Product	deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK077244.1 and AK153285.1. On Apr 25, 2009 this sequence version replaced NM_023595.5. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK153285.1, AK077244.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCCTGCT CGGAAGATGC CGCGGCCGTC TCTGCCTCCA AGAGGGCTCG AGCGGAGGAT 
GGCGCTTCTC TGCGCTTCGT GCGGCTCTCG GAGCACGCCA CGGCGCCCAC CCGCGGGTCC 
GCGCGCGCTG CCGGCTACGA CCTATTCAGT GCCTATGATT ATACAATATC ACCCATGGAG 
AAAGCCATCG TGAAGACAGA CATTCAGATA GCTGTCCCTT CTGGGTGCTA TGGAAGAGTA 
GCTCCACGTT CTGGCTTGGC TGTAAAGCAC TTCATAGATG TAGGAGCTGG TGTCATAGAC 
GAGGATTACA GAGGAAACGT TGGGGTCGTG CTGTTTAACT TTGGGAAAGA GAAGTTTGAA 
GTGAAAAAAG GTGATCGGAT TGCGCAGCTC ATCTGTGAGC GGATTTCTTA TCCAGACTTA 
GAGGAAGTGC AGACCCTGGA TGACACCGAG AGAGGCTCAG GAGGCTTCGG CTCCACCGGG 
AAGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076084.2
CDS	234..722
Translation	MPCSEDAAAVSASKRARAEDGASLRFVRLSEHATAPTRGSARAAGYDLFSAYDYTISPMEKAIVKTDIQIAVPSGCYGRVAPRSGLAVKHFIDVGAGVIDEDYRGNVGVVLFNFGKEKFEVKKGDRIAQLICERISYPDLEEVQTLDDTERGSGGFGSTGKN

Target ORF information:

RefSeq Version	NM_023595.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus deoxyuridine triphosphatase (Dut), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023595.6

ORF Insert Sequence:

ATGCCCTGCT CGGAAGATGC CGCGGCCGTC TCTGCCTCCA AGAGGGCTCG AGCGGAGGAT 
GGCGCTTCTC TGCGCTTCGT GCGGCTCTCG GAGCACGCCA CGGCGCCCAC CCGCGGGTCC 
GCGCGCGCTG CCGGCTACGA CCTATTCAGT GCCTATGATT ATACAATATC ACCCATGGAG 
AAAGCCATCG TGAAGACAGA CATTCAGATA GCTGTCCCTT CTGGGTGCTA TGGAAGAGTA 
GCTCCACGTT CTGGCTTGGC TGTAAAGCAC TTCATAGATG TAGGAGCTGG TGTCATAGAC 
GAGGATTACA GAGGAAACGT TGGGGTCGTG CTGTTTAACT TTGGGAAAGA GAAGTTTGAA 
GTGAAAAAAG GTGATCGGAT TGCGCAGCTC ATCTGTGAGC GGATTTCTTA TCCAGACTTA 
GAGGAAGTGC AGACCCTGGA TGACACCGAG AGAGGCTCAG GAGGCTTCGG CTCCACCGGG 
AAGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dut ( NM_001159646.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153118.1 Mus musculus deoxyuridine triphosphatase (Dut), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07691
Clone ID Related Accession (Same CDS sequence)	NM_001159646.1
Accession Version	NM_001159646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Mus musculus(house mouse)
Product	deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK135438.1 and AK153285.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK135438.1, AK017216.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCTCTCC TAAGCGTGCT GCTGCGCGCC CGCCTGCAGG CCGCTCTGCT CCGCGGGCGT 
GCGCTCGGGT CGGCGCGGAG CCGGAGCTGC CGAGGAAGCC GAGGAGCTCC GGCCGGGAGC 
GCGCGCGCTG ATGCCGCGGC CGTCTCTGCC TCCAAGAGGG CTCGAGCGGA GGATGGCGCT 
TCTCTGCGCT TCGTGCGGCT CTCGGAGCAC GCCACGGCGC CCACCCGCGG GTCCGCGCGC 
GCTGCCGGCT ACGACCTATT CAGTGCCTAT GATTATACAA TATCACCCAT GGAGAAAGCC 
ATCGTGAAGA CAGACATTCA GATAGCTGTC CCTTCTGGGT GCTATGGAAG AGTAGCTCCA 
CGTTCTGGCT TGGCTGTAAA GCACTTCATA GATGTAGGAG CTGGTGTCAT AGACGAGGAT 
TACAGAGGAA ACGTTGGGGT CGTGCTGTTT AACTTTGGGA AAGAGAAGTT TGAAGTGAAA 
AAAGGTGATC GGATTGCGCA GCTCATCTGT GAGCGGATTT CTTATCCAGA CTTAGAGGAA 
GTGCAGACCC TGGATGACAC CGAGAGAGGC TCAGGAGGCT TCGGCTCCAC CGGGAAGAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153118.1
CDS	25..627
Translation	MPLLSVLLRARLQAALLRGRALGSARSRSCRGSRGAPAGSARADAAAVSASKRARAEDGASLRFVRLSEHATAPTRGSARAAGYDLFSAYDYTISPMEKAIVKTDIQIAVPSGCYGRVAPRSGLAVKHFIDVGAGVIDEDYRGNVGVVLFNFGKEKFEVKKGDRIAQLICERISYPDLEEVQTLDDTERGSGGFGSTGKN

Target ORF information:

RefSeq Version	NM_001159646.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus deoxyuridine triphosphatase (Dut), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159646.1

ORF Insert Sequence:

ATGCCTCTCC TAAGCGTGCT GCTGCGCGCC CGCCTGCAGG CCGCTCTGCT CCGCGGGCGT 
GCGCTCGGGT CGGCGCGGAG CCGGAGCTGC CGAGGAAGCC GAGGAGCTCC GGCCGGGAGC 
GCGCGCGCTG ATGCCGCGGC CGTCTCTGCC TCCAAGAGGG CTCGAGCGGA GGATGGCGCT 
TCTCTGCGCT TCGTGCGGCT CTCGGAGCAC GCCACGGCGC CCACCCGCGG GTCCGCGCGC 
GCTGCCGGCT ACGACCTATT CAGTGCCTAT GATTATACAA TATCACCCAT GGAGAAAGCC 
ATCGTGAAGA CAGACATTCA GATAGCTGTC CCTTCTGGGT GCTATGGAAG AGTAGCTCCA 
CGTTCTGGCT TGGCTGTAAA GCACTTCATA GATGTAGGAG CTGGTGTCAT AGACGAGGAT 
TACAGAGGAA ACGTTGGGGT CGTGCTGTTT AACTTTGGGA AAGAGAAGTT TGAAGTGAAA 
AAAGGTGATC GGATTGCGCA GCTCATCTGT GAGCGGATTT CTTATCCAGA CTTAGAGGAA 
GTGCAGACCC TGGATGACAC CGAGAGAGGC TCAGGAGGCT TCGGCTCCAC CGGGAAGAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RORalpha coordinates reciprocal signaling in cerebellar development through sonic hedgehog and calcium-dependent pathways.
Neuron40(6)1119-31(2003 Dec)
Gold DA,Baek SH,Schork NJ,Rose DW,Larsen DD,Sachs BD,Rosenfeld MG,Hamilton BA

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

Novel gain of function activity of p53 mutants: activation of the dUTPase gene expression leading to resistance to 5-fluorouracil.
Oncogene21(30)4595-600(2002 Jul)
Pugacheva EN,Ivanov AV,Kravchenko JE,Kopnin BP,Levine AJ,Chumakov PM

Embryonic lethality and vascular defects in mice lacking the Notch ligand Jagged1.
Human molecular genetics8(5)723-30(1999 May)
Xue Y,Gao X,Lindsell CE,Norton CR,Chang B,Hicks C,Gendron-Maguire M,Rand EB,Weinmaster G,Gridley T

Cloning and expression of the mouse deoxyuridine triphosphate nucleotidohydrolase gene: differs from the rat enzyme in that it lacks nuclear receptor interacting LXXLL motif.
Gene expression8(4)231-46(1999)
Kan L,Jain S,Cook W,Cao WQ,Usuda N,Yeldandi AV,Rao MS,Kanwar YS,Reddy JK