Cela1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cela1
Entrez Gene ID	109901
Full Name	chymotrypsin-like elastase family, member 1
Synonyms	1810009A17Rik,1810062B19Rik,Ela-1,Ela1,PC-TsF
General protein information	Preferred Names chymotrypsin-like elastase family, member 1 Names chymotrypsin-like elastase family member 1 elastase 1, pancreatic elastase-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 F1\|15 56.38 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_033612.2	NP_291090.2	chymotrypsin-like elastase family member 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CELA1	NP_001962.3
Canis lupus familiaris (dog)	CELA1	NP_001003007.1
Rattus norvegicus (Norway rat)	Cela1	NP_036684.1
Xenopus tropicalis (tropical clawed frog)	LOC496972	NP_001011481.1
Xenopus tropicalis (tropical clawed frog)	ela1	NP_001025669.1
	LOC695507	XP_001088682.1
Xenopus tropicalis (tropical clawed frog)	cela1	NP_001011493.1
Xenopus tropicalis (tropical clawed frog)	LOC101730694	XP_004911959.1
Xenopus tropicalis (tropical clawed frog)	cela1	XP_002933738.2
Pan troglodytes (chimpanzee)	CELA1	XP_001142473.2
Mus musculus (house mouse)	Cela1	NP_291090.2
Xenopus tropicalis (tropical clawed frog)	LOC100135144	XP_002933737.2
Bos taurus (cattle)	CELA1	NP_776473.1
Gallus gallus (chicken)	CELA1	NP_001096672.1

Gene Ontology
Bibliography

Related articles in PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

Stretch regulates expression and binding of chymotrypsin-like elastase 1 in the postnatal lung.
Joshi R, Liu S, Brown MD, Young SM, Batie M, Kofron JM, Xu Y, Weaver TE, Apsley K, Varisco BM
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(2)590-600(2016 Feb)

von Hippel-Lindau gene disruption in mouse pancreatic progenitors and its consequences on endocrine differentiation in vivo: importance of HIF1-? and VEGF-A upregulation.
Soggia A, Ramond C, Akiyama H, Scharfmann R, Duvillie B
Diabetologia57(11)2348-56(2014 Nov)

Dynamic expression of chymotrypsin-like elastase 1 over the course of murine lung development.
Liu S, Young SM, Varisco BM
American journal of physiology. Lung cellular and molecular physiology306(12)L1104-16(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The stretch-dependent expression and binding kinetics of Cela1 indicate an important role in stretch-dependent remodeling of the peripheral lung during development and regeneration.
Title: Stretch regulates expression and binding of chymotrypsin-like elastase 1 in the postnatal lung.

defines the temporal-spatial pattern of elastin remodeling during lung development, demonstrates an association of this remodeling with chymotrypsin-like elastase 1
Title: Dynamic expression of chymotrypsin-like elastase 1 over the course of murine lung development.

The enzymatic properties and kinetic profiles for mouse pancreatic elastase were similar to other known serine elastases.
Title: Enzyme kinetics and characterization of mouse pancreatic elastase.

Glucocorticoid receptor-null lungs included Chi3l3 (11-fold, P < 0.05), a macrophage inflammatory response gene and Ela1 (9.4-fold, P < 0.05), an extracellular matrix remodeling enzyme.
Title: Identification of glucocorticoid-regulated genes that control cell proliferation during murine respiratory development.

The following Cela1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cela1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08889	NM_033612.2 Latest version!	Mus musculus chymotrypsin-like elastase family, member 1 (Cela1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cela1 ( NM_033612.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_291090.2 Mus musculus chymotrypsin-like elastase family, member 1 (Cela1), mRNA.

Price & Availability↑

CloneID	OMu08889
Clone ID Related Accession (Same CDS sequence)	NM_033612.2
Accession Version	NM_033612.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	chymotrypsin-like elastase family member 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB951068.1, AK007392.1 and BP769372.1. On Jan 18, 2008 this sequence version replaced NM_033612.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK007392.1, BC011218.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849388 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGCGCT TCCTGGTGTT TGCTTCCCTG GTCCTGTGTG GACACAGTAC CGAGGACGTT 
CCGGAAACTG ACGCCCGCGT GGTTGGAGGG GCTGAAGCCC GGAGGAACTC TTGGCCGTCT 
CAGATTTCCC TCCAGTACCA GTATGGAGGA TCATGGCACC ACACCTGTGG AGGCACCCTC 
ATCCGAAGCA ACTGGGTGAT GACCGCTGCT CACTGTGTGG ACAGCCCCAT GACTTATCGA 
GTGGTTGTCG GAGAGCACAA CCTGAGCCAG AATGACGGCA CCGAGCAGTA TGTGAACGTG 
CAGAAGATTG TGTCACACCC CTACTGGAAC AAGAACAACG TGGTTGCAGG CTATGACATC 
GCCCTGCTGC GCTTGGCCAA GAGTGTTACA CTCAATAACT ATGTCCAGCT GGGTGTTCTG 
CCCCGGGAGG GAACCATCCT GGCTAACAAC TCTCCCTGCT ACATCACAGG CTGGGGAAGA 
ACCAGAACCA ATGGGGAGCT GGCCCAGACC CTGCAGCAGG CATACCTGCC CAGCGTGTCC 
TATAGCATCT GCTCCAGCTC CTCTTACTGG GGCTCCTCTG TGAAGAATAC CATGGTGTGC 
GCTGGTGGAG ATGGAGTCCG CTCTGGATGC CAGGGTGATT CTGGGGGACC CCTCCACTGC 
ATGGTGAACG GTCAGTATGC TGTCCACGGA GTGACCAGCT TTGTGTCCAG CATGGGCTGT 
AATGTCGCCA GGAAGCCCAC CGTCTTCACC AGAGTCTCTG CTTACATTTC CTGGATGAAT 
AATGTCATTG CCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_291090.2
CDS	28..828
Translation	MLRFLVFASLVLCGHSTEDVPETDARVVGGAEARRNSWPSQISLQYQYGGSWHHTCGGTLIRSNWVMTAAHCVDSPMTYRVVVGEHNLSQNDGTEQYVNVQKIVSHPYWNKNNVVAGYDIALLRLAKSVTLNNYVQLGVLPREGTILANNSPCYITGWGRTRTNGELAQTLQQAYLPSVSYSICSSSSYWGSSVKNTMVCAGGDGVRSGCQGDSGGPLHCMVNGQYAVHGVTSFVSSMGCNVARKPTVFTRVSAYISWMNNVIASN

Target ORF information:

RefSeq Version	NM_033612.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chymotrypsin-like elastase family, member 1 (Cela1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033612.2

ORF Insert Sequence:

ATGTTGCGCT TCCTGGTGTT TGCTTCCCTG GTCCTGTGTG GACACAGTAC CGAGGACGTT 
CCGGAAACTG ACGCCCGCGT GGTTGGAGGG GCTGAAGCCC GGAGGAACTC TTGGCCGTCT 
CAGATTTCCC TCCAGTACCA GTATGGAGGA TCATGGCACC ACACCTGTGG AGGCACCCTC 
ATCCGAAGCA ACTGGGTGAT GACCGCTGCT CACTGTGTGG ACAGCCCCAT GACTTATCGA 
GTGGTTGTCG GAGAGCACAA CCTGAGCCAG AATGACGGCA CCGAGCAGTA TGTGAACGTG 
CAGAAGATTG TGTCACACCC CTACTGGAAC AAGAACAACG TGGTTGCAGG CTATGACATC 
GCCCTGCTGC GCTTGGCCAA GAGTGTTACA CTCAATAACT ATGTCCAGCT GGGTGTTCTG 
CCCCGGGAGG GAACCATCCT GGCTAACAAC TCTCCCTGCT ACATCACAGG CTGGGGAAGA 
ACCAGAACCA ATGGGGAGCT GGCCCAGACC CTGCAGCAGG CATACCTGCC CAGCGTGTCC 
TATAGCATCT GCTCCAGCTC CTCTTACTGG GGCTCCTCTG TGAAGAATAC CATGGTGTGC 
GCTGGTGGAG ATGGAGTCCG CTCTGGATGC CAGGGTGATT CTGGGGGACC CCTCCACTGC 
ATGGTGAACG GTCAGTATGC TGTCCACGGA GTGACCAGCT TTGTGTCCAG CATGGGCTGT 
AATGTCGCCA GGAAGCCCAC CGTCTTCACC AGAGTCTCTG CTTACATTTC CTGGATGAAT 
AATGTCATTG CCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

Stretch regulates expression and binding of chymotrypsin-like elastase 1 in the postnatal lung.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(2)590-600(2016 Feb)
Joshi R,Liu S,Brown MD,Young SM,Batie M,Kofron JM,Xu Y,Weaver TE,Apsley K,Varisco BM

von Hippel-Lindau gene disruption in mouse pancreatic progenitors and its consequences on endocrine differentiation in vivo: importance of HIF1-? and VEGF-A upregulation.
Diabetologia57(11)2348-56(2014 Nov)
Soggia A,Ramond C,Akiyama H,Scharfmann R,Duvillie B

Dynamic expression of chymotrypsin-like elastase 1 over the course of murine lung development.
American journal of physiology. Lung cellular and molecular physiology306(12)L1104-16(2014 Jun)
Liu S,Young SM,Varisco BM