B3gnt5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	B3gnt5
Entrez Gene ID	108105
Full Name	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
Synonyms	beta3GnT5
General protein information	Preferred Names UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 Names lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase BGnT-5 UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5 beta-1,3-Gn-T5 beta-1,3-N-acetylglucosaminyltransferase 5 beta3Gn-T5 lactotriaosylceramide synthase lc(3)Cer synthase lc3 synthase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_011245803.1	XP_011244105.1	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase isoform X1
NM_001159408.1	NP_001152880.1	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
NM_054052.3	NP_473393.2	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
XM_030248916.1	XP_030104776.1	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase isoform X1
XM_006521706.3	XP_006521769.1	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase isoform X1
XM_006521706.2	XP_006521769.1	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase isoform X1
NM_001159407.1	NP_001152879.1	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	B3GNT5	NP_001107233.1
Danio rerio (zebrafish)	b3gnt5a	NP_942577.1
Canis lupus familiaris (dog)	B3GNT5	XP_005639817.1
Xenopus tropicalis (tropical clawed frog)	b3gnt5	NP_989240.1
	B3GNT5	XP_001106314.1
Homo sapiens (human)	B3GNT5	NP_114436.1
Pan troglodytes (chimpanzee)	B3GNT5	XP_001134746.2
Bos taurus (cattle)	B3GNT5	NP_001070447.1
Mus musculus (house mouse)	B3gnt5	NP_001152879.1
Rattus norvegicus (Norway rat)	B3gnt5	NP_446384.1

Gene Ontology
Bibliography

Related articles in PubMed

Multiple phenotypic changes in mice after knockout of the B3gnt5 gene, encoding Lc3 synthase--a key enzyme in lacto-neolacto ganglioside synthesis.
Kuan CT, Chang J, Mansson JE, Li J, Pegram C, Fredman P, McLendon RE, Bigner DD
BMC developmental biology10114(2010 Nov)

Lack of lacto/neolacto-glycolipids enhances the formation of glycolipid-enriched microdomains, facilitating B cell activation.
Togayachi A, Kozono Y, Ikehara Y, Ito H, Suzuki N, Tsunoda Y, Abe S, Sato T, Nakamura K, Suzuki M, Goda HM, Ito M, Kudo T, Takahashi S, Narimatsu H
Proceedings of the National Academy of Sciences of the United States of America107(26)11900-5(2010 Jun)

The Lc3-synthase gene B3gnt5 is essential to pre-implantation development of the murine embryo.
Biellmann F, H?lsmeier AJ, Zhou D, Cinelli P, Hennet T
BMC developmental biology8109(2008 Nov)

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Piao Y, Ko NT, Lim MK, Ko MS
Genome research11(9)1553-8(2001 Sep)

Cloning of a mouse beta 1,3 N-acetylglucosaminyltransferase GlcNAc(beta 1,3)Gal(beta 1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis.
Henion TR, Zhou D, Wolfer DP, Jungalwala FB, Hennet T
The Journal of biological chemistry276(32)30261-9(2001 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrate that Lc3 synthase and lacto-neolacto series gangliosides are essential to multiple physiological functions, especially the control of reproductive output, and spleen B-cell abnormality.
Title: Multiple phenotypic changes in mice after knockout of the B3gnt5 gene, encoding Lc3 synthase--a key enzyme in lacto-neolacto ganglioside synthesis.

B3gnt5 is essential to pre-implantation development of the murine embryo.
Title: The Lc3-synthase gene B3gnt5 is essential to pre-implantation development of the murine embryo.

The following B3gnt5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B3gnt5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07444	NM_001159408.1 Latest version!	Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu07444	NM_001159407.1 Latest version!	Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu07444	NM_054052.3 Latest version!	Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu07444	XM_006521706.3 Latest version!	Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu07444	XM_030248916.1 Latest version!	Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu07444	XM_011245803.1 Latest version!	Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
View Old Accession Versions >>
OMu07444	XM_006521706.2	Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B3gnt5 ( NM_001159408.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001152880.1 Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu07444
Clone ID Related Accession (Same CDS sequence)	XM_011245803.1 , NM_001159408.1 , NM_054052.3 , XM_030248916.1 , XM_006521706.3 , XM_006521706.2 , NM_001159407.1
Accession Version	NM_001159408.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK037166.1, AK048010.1, BC063076.1 and BB667148.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK037166.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGACTGT TTGTTAGCAG AAGAGTCAAA AGATGGAAAA TTTTTCACTT TTTTGTCACT 
TGTTTTATAT TAAGCTTCAT GGTTTTTTGG AGCCCAATCA ATAATTACAT CATGAGCCAT 
ATGAAGTCCT ACTCCTACAG ATACCTCGTA AATAGCTATG GCTTTGTAAA CAATTCCCTG 
TCTCTCAAGC ACAGCTCTGT GCAGCCTCAC TACCCATACT TGATCAACCA CAGAGAGAAG 
TGTCAGGCTC AAGATGTCCT CCTCTTACTG TTTATAAAGA CTGCCCCTGA AAACTATGGC 
CGACGTTCTG CAATCAGAAA AACGTGGGGC AATGAGAACT ATGTTCAGTC TCAACTCAAT 
GCCAACATCA AAATTCTGTT TGCATTAGGA ACTCCTGGTC CACTGAAGGG AAAAGAACTG 
CAAAAAAGAC TAATCGGGGA AGATCAAGTG TACAAGGATA TAATTCAGCA AGATTTCATT 
GATTCTTTCC ACAATCTTAC TTCTAAATTC CTTCTTCAGT TCAGCTGGGC AAATACCTTT 
TGTCCACATG CCAAATTCCT GATGACTGCT GATGATGATA TATTTATCCA CATGCCAAAT 
CTCATTGAAT ATCTTCAAGG GCTAGAGCAG ATTGGAGTTC GAGACTTTTG GATTGGTCAC 
GTTCATCGAG GTGGCCCTCC TGTTAGGGAT AAAAGCAGCA AATACTATGT TCCCTATGAA 
ATGTACAAGT GGCCAGCCTA CCCTGACTAT ACAGCTGGTG CTGCCTATGT TGTCTCCAGA 
GATGTAGCTG CCAAAATCTA TGAGGCATCG CAGACACTGA ATTCCAGTAT GTACATAGAT 
GATGTATTCA TGGGCCTCTG TGCCAATAAA GTGGGAATCT TGCCACAGGA CCATGTATTT 
TTCTCTGGGG AAGGGAAAAT TCCTTATCAC CCCTGCATCT ATGAAAAGAT GATGACATCT 
CACGGACACT TACAAGATCT GCAGGACCTC TGGATAGAGG CTACACATCC TAAAGTAAAG 
AACATTTCAA AAGGGTTTTT TGGTCAAATA TACTGCAGGT TAATTAAGAT AGTTCTTCTC 
TGCAGACTGA CTTACAGGAA TTCATACCCT TGTTGGGCTG CATTTGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152880.1
CDS	949..2079
Translation	MRLFVSRRVKRWKIFHFFVTCFILSFMVFWSPINNYIMSHMKSYSYRYLVNSYGFVNNSLSLKHSSVQPHYPYLINHREKCQAQDVLLLLFIKTAPENYGRRSAIRKTWGNENYVQSQLNANIKILFALGTPGPLKGKELQKRLIGEDQVYKDIIQQDFIDSFHNLTSKFLLQFSWANTFCPHAKFLMTADDDIFIHMPNLIEYLQGLEQIGVRDFWIGHVHRGGPPVRDKSSKYYVPYEMYKWPAYPDYTAGAAYVVSRDVAAKIYEASQTLNSSMYIDDVFMGLCANKVGILPQDHVFFSGEGKIPYHPCIYEKMMTSHGHLQDLQDLWIEATHPKVKNISKGFFGQIYCRLIKIVLLCRLTYRNSYPCWAAFA

Target ORF information:

RefSeq Version	NM_001159408.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159408.1

ORF Insert Sequence:

ATGAGACTGT TTGTTAGCAG AAGAGTCAAA AGATGGAAAA TTTTTCACTT TTTTGTCACT 
TGTTTTATAT TAAGCTTCAT GGTTTTTTGG AGCCCAATCA ATAATTACAT CATGAGCCAT 
ATGAAGTCCT ACTCCTACAG ATACCTCGTA AATAGCTATG GCTTTGTAAA CAATTCCCTG 
TCTCTCAAGC ACAGCTCTGT GCAGCCTCAC TACCCATACT TGATCAACCA CAGAGAGAAG 
TGTCAGGCTC AAGATGTCCT CCTCTTACTG TTTATAAAGA CTGCCCCTGA AAACTATGGC 
CGACGTTCTG CAATCAGAAA AACGTGGGGC AATGAGAACT ATGTTCAGTC TCAACTCAAT 
GCCAACATCA AAATTCTGTT TGCATTAGGA ACTCCTGGTC CACTGAAGGG AAAAGAACTG 
CAAAAAAGAC TAATCGGGGA AGATCAAGTG TACAAGGATA TAATTCAGCA AGATTTCATT 
GATTCTTTCC ACAATCTTAC TTCTAAATTC CTTCTTCAGT TCAGCTGGGC AAATACCTTT 
TGTCCACATG CCAAATTCCT GATGACTGCT GATGATGATA TATTTATCCA CATGCCAAAT 
CTCATTGAAT ATCTTCAAGG GCTAGAGCAG ATTGGAGTTC GAGACTTTTG GATTGGTCAC 
GTTCATCGAG GTGGCCCTCC TGTTAGGGAT AAAAGCAGCA AATACTATGT TCCCTATGAA 
ATGTACAAGT GGCCAGCCTA CCCTGACTAT ACAGCTGGTG CTGCCTATGT TGTCTCCAGA 
GATGTAGCTG CCAAAATCTA TGAGGCATCG CAGACACTGA ATTCCAGTAT GTACATAGAT 
GATGTATTCA TGGGCCTCTG TGCCAATAAA GTGGGAATCT TGCCACAGGA CCATGTATTT 
TTCTCTGGGG AAGGGAAAAT TCCTTATCAC CCCTGCATCT ATGAAAAGAT GATGACATCT 
CACGGACACT TACAAGATCT GCAGGACCTC TGGATAGAGG CTACACATCC TAAAGTAAAG 
AACATTTCAA AAGGGTTTTT TGGTCAAATA TACTGCAGGT TAATTAAGAT AGTTCTTCTC 
TGCAGACTGA CTTACAGGAA TTCATACCCT TGTTGGGCTG CATTTGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B3gnt5 ( NM_054052.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_473393.2 Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07444
Clone ID Related Accession (Same CDS sequence)	XM_011245803.1 , NM_001159408.1 , NM_054052.3 , XM_030248916.1 , XM_006521706.3 , XM_006521706.2 , NM_001159407.1
Accession Version	NM_054052.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK083839.1, BC063076.1 and BB667148.1. On Apr 16, 2009 this sequence version replaced NM_054052.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK083839.1, BC063076.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGACTGT TTGTTAGCAG AAGAGTCAAA AGATGGAAAA TTTTTCACTT TTTTGTCACT 
TGTTTTATAT TAAGCTTCAT GGTTTTTTGG AGCCCAATCA ATAATTACAT CATGAGCCAT 
ATGAAGTCCT ACTCCTACAG ATACCTCGTA AATAGCTATG GCTTTGTAAA CAATTCCCTG 
TCTCTCAAGC ACAGCTCTGT GCAGCCTCAC TACCCATACT TGATCAACCA CAGAGAGAAG 
TGTCAGGCTC AAGATGTCCT CCTCTTACTG TTTATAAAGA CTGCCCCTGA AAACTATGGC 
CGACGTTCTG CAATCAGAAA AACGTGGGGC AATGAGAACT ATGTTCAGTC TCAACTCAAT 
GCCAACATCA AAATTCTGTT TGCATTAGGA ACTCCTGGTC CACTGAAGGG AAAAGAACTG 
CAAAAAAGAC TAATCGGGGA AGATCAAGTG TACAAGGATA TAATTCAGCA AGATTTCATT 
GATTCTTTCC ACAATCTTAC TTCTAAATTC CTTCTTCAGT TCAGCTGGGC AAATACCTTT 
TGTCCACATG CCAAATTCCT GATGACTGCT GATGATGATA TATTTATCCA CATGCCAAAT 
CTCATTGAAT ATCTTCAAGG GCTAGAGCAG ATTGGAGTTC GAGACTTTTG GATTGGTCAC 
GTTCATCGAG GTGGCCCTCC TGTTAGGGAT AAAAGCAGCA AATACTATGT TCCCTATGAA 
ATGTACAAGT GGCCAGCCTA CCCTGACTAT ACAGCTGGTG CTGCCTATGT TGTCTCCAGA 
GATGTAGCTG CCAAAATCTA TGAGGCATCG CAGACACTGA ATTCCAGTAT GTACATAGAT 
GATGTATTCA TGGGCCTCTG TGCCAATAAA GTGGGAATCT TGCCACAGGA CCATGTATTT 
TTCTCTGGGG AAGGGAAAAT TCCTTATCAC CCCTGCATCT ATGAAAAGAT GATGACATCT 
CACGGACACT TACAAGATCT GCAGGACCTC TGGATAGAGG CTACACATCC TAAAGTAAAG 
AACATTTCAA AAGGGTTTTT TGGTCAAATA TACTGCAGGT TAATTAAGAT AGTTCTTCTC 
TGCAGACTGA CTTACAGGAA TTCATACCCT TGTTGGGCTG CATTTGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_473393.2
CDS	828..1958
Translation	MRLFVSRRVKRWKIFHFFVTCFILSFMVFWSPINNYIMSHMKSYSYRYLVNSYGFVNNSLSLKHSSVQPHYPYLINHREKCQAQDVLLLLFIKTAPENYGRRSAIRKTWGNENYVQSQLNANIKILFALGTPGPLKGKELQKRLIGEDQVYKDIIQQDFIDSFHNLTSKFLLQFSWANTFCPHAKFLMTADDDIFIHMPNLIEYLQGLEQIGVRDFWIGHVHRGGPPVRDKSSKYYVPYEMYKWPAYPDYTAGAAYVVSRDVAAKIYEASQTLNSSMYIDDVFMGLCANKVGILPQDHVFFSGEGKIPYHPCIYEKMMTSHGHLQDLQDLWIEATHPKVKNISKGFFGQIYCRLIKIVLLCRLTYRNSYPCWAAFA

Target ORF information:

RefSeq Version	NM_054052.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_054052.3

ORF Insert Sequence:

ATGAGACTGT TTGTTAGCAG AAGAGTCAAA AGATGGAAAA TTTTTCACTT TTTTGTCACT 
TGTTTTATAT TAAGCTTCAT GGTTTTTTGG AGCCCAATCA ATAATTACAT CATGAGCCAT 
ATGAAGTCCT ACTCCTACAG ATACCTCGTA AATAGCTATG GCTTTGTAAA CAATTCCCTG 
TCTCTCAAGC ACAGCTCTGT GCAGCCTCAC TACCCATACT TGATCAACCA CAGAGAGAAG 
TGTCAGGCTC AAGATGTCCT CCTCTTACTG TTTATAAAGA CTGCCCCTGA AAACTATGGC 
CGACGTTCTG CAATCAGAAA AACGTGGGGC AATGAGAACT ATGTTCAGTC TCAACTCAAT 
GCCAACATCA AAATTCTGTT TGCATTAGGA ACTCCTGGTC CACTGAAGGG AAAAGAACTG 
CAAAAAAGAC TAATCGGGGA AGATCAAGTG TACAAGGATA TAATTCAGCA AGATTTCATT 
GATTCTTTCC ACAATCTTAC TTCTAAATTC CTTCTTCAGT TCAGCTGGGC AAATACCTTT 
TGTCCACATG CCAAATTCCT GATGACTGCT GATGATGATA TATTTATCCA CATGCCAAAT 
CTCATTGAAT ATCTTCAAGG GCTAGAGCAG ATTGGAGTTC GAGACTTTTG GATTGGTCAC 
GTTCATCGAG GTGGCCCTCC TGTTAGGGAT AAAAGCAGCA AATACTATGT TCCCTATGAA 
ATGTACAAGT GGCCAGCCTA CCCTGACTAT ACAGCTGGTG CTGCCTATGT TGTCTCCAGA 
GATGTAGCTG CCAAAATCTA TGAGGCATCG CAGACACTGA ATTCCAGTAT GTACATAGAT 
GATGTATTCA TGGGCCTCTG TGCCAATAAA GTGGGAATCT TGCCACAGGA CCATGTATTT 
TTCTCTGGGG AAGGGAAAAT TCCTTATCAC CCCTGCATCT ATGAAAAGAT GATGACATCT 
CACGGACACT TACAAGATCT GCAGGACCTC TGGATAGAGG CTACACATCC TAAAGTAAAG 
AACATTTCAA AAGGGTTTTT TGGTCAAATA TACTGCAGGT TAATTAAGAT AGTTCTTCTC 
TGCAGACTGA CTTACAGGAA TTCATACCCT TGTTGGGCTG CATTTGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B3gnt5 ( NM_001159407.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001152879.1 Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07444
Clone ID Related Accession (Same CDS sequence)	XM_011245803.1 , NM_001159408.1 , NM_054052.3 , XM_030248916.1 , XM_006521706.3 , XM_006521706.2 , NM_001159407.1
Accession Version	NM_001159407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK083839.1, CN533455.1, AK037166.1, AK048010.1, BC063076.1 and BB667148.1. Transcript Variant: This variant (1) represents the longest transcript. All three variants encode the same protein. ##Evidence-Data-START## Transcript exon combination :: CN533455.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGACTGT TTGTTAGCAG AAGAGTCAAA AGATGGAAAA TTTTTCACTT TTTTGTCACT 
TGTTTTATAT TAAGCTTCAT GGTTTTTTGG AGCCCAATCA ATAATTACAT CATGAGCCAT 
ATGAAGTCCT ACTCCTACAG ATACCTCGTA AATAGCTATG GCTTTGTAAA CAATTCCCTG 
TCTCTCAAGC ACAGCTCTGT GCAGCCTCAC TACCCATACT TGATCAACCA CAGAGAGAAG 
TGTCAGGCTC AAGATGTCCT CCTCTTACTG TTTATAAAGA CTGCCCCTGA AAACTATGGC 
CGACGTTCTG CAATCAGAAA AACGTGGGGC AATGAGAACT ATGTTCAGTC TCAACTCAAT 
GCCAACATCA AAATTCTGTT TGCATTAGGA ACTCCTGGTC CACTGAAGGG AAAAGAACTG 
CAAAAAAGAC TAATCGGGGA AGATCAAGTG TACAAGGATA TAATTCAGCA AGATTTCATT 
GATTCTTTCC ACAATCTTAC TTCTAAATTC CTTCTTCAGT TCAGCTGGGC AAATACCTTT 
TGTCCACATG CCAAATTCCT GATGACTGCT GATGATGATA TATTTATCCA CATGCCAAAT 
CTCATTGAAT ATCTTCAAGG GCTAGAGCAG ATTGGAGTTC GAGACTTTTG GATTGGTCAC 
GTTCATCGAG GTGGCCCTCC TGTTAGGGAT AAAAGCAGCA AATACTATGT TCCCTATGAA 
ATGTACAAGT GGCCAGCCTA CCCTGACTAT ACAGCTGGTG CTGCCTATGT TGTCTCCAGA 
GATGTAGCTG CCAAAATCTA TGAGGCATCG CAGACACTGA ATTCCAGTAT GTACATAGAT 
GATGTATTCA TGGGCCTCTG TGCCAATAAA GTGGGAATCT TGCCACAGGA CCATGTATTT 
TTCTCTGGGG AAGGGAAAAT TCCTTATCAC CCCTGCATCT ATGAAAAGAT GATGACATCT 
CACGGACACT TACAAGATCT GCAGGACCTC TGGATAGAGG CTACACATCC TAAAGTAAAG 
AACATTTCAA AAGGGTTTTT TGGTCAAATA TACTGCAGGT TAATTAAGAT AGTTCTTCTC 
TGCAGACTGA CTTACAGGAA TTCATACCCT TGTTGGGCTG CATTTGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152879.1
CDS	1152..2282
Translation	MRLFVSRRVKRWKIFHFFVTCFILSFMVFWSPINNYIMSHMKSYSYRYLVNSYGFVNNSLSLKHSSVQPHYPYLINHREKCQAQDVLLLLFIKTAPENYGRRSAIRKTWGNENYVQSQLNANIKILFALGTPGPLKGKELQKRLIGEDQVYKDIIQQDFIDSFHNLTSKFLLQFSWANTFCPHAKFLMTADDDIFIHMPNLIEYLQGLEQIGVRDFWIGHVHRGGPPVRDKSSKYYVPYEMYKWPAYPDYTAGAAYVVSRDVAAKIYEASQTLNSSMYIDDVFMGLCANKVGILPQDHVFFSGEGKIPYHPCIYEKMMTSHGHLQDLQDLWIEATHPKVKNISKGFFGQIYCRLIKIVLLCRLTYRNSYPCWAAFA

Target ORF information:

RefSeq Version	NM_001159407.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159407.1

ORF Insert Sequence:

ATGAGACTGT TTGTTAGCAG AAGAGTCAAA AGATGGAAAA TTTTTCACTT TTTTGTCACT 
TGTTTTATAT TAAGCTTCAT GGTTTTTTGG AGCCCAATCA ATAATTACAT CATGAGCCAT 
ATGAAGTCCT ACTCCTACAG ATACCTCGTA AATAGCTATG GCTTTGTAAA CAATTCCCTG 
TCTCTCAAGC ACAGCTCTGT GCAGCCTCAC TACCCATACT TGATCAACCA CAGAGAGAAG 
TGTCAGGCTC AAGATGTCCT CCTCTTACTG TTTATAAAGA CTGCCCCTGA AAACTATGGC 
CGACGTTCTG CAATCAGAAA AACGTGGGGC AATGAGAACT ATGTTCAGTC TCAACTCAAT 
GCCAACATCA AAATTCTGTT TGCATTAGGA ACTCCTGGTC CACTGAAGGG AAAAGAACTG 
CAAAAAAGAC TAATCGGGGA AGATCAAGTG TACAAGGATA TAATTCAGCA AGATTTCATT 
GATTCTTTCC ACAATCTTAC TTCTAAATTC CTTCTTCAGT TCAGCTGGGC AAATACCTTT 
TGTCCACATG CCAAATTCCT GATGACTGCT GATGATGATA TATTTATCCA CATGCCAAAT 
CTCATTGAAT ATCTTCAAGG GCTAGAGCAG ATTGGAGTTC GAGACTTTTG GATTGGTCAC 
GTTCATCGAG GTGGCCCTCC TGTTAGGGAT AAAAGCAGCA AATACTATGT TCCCTATGAA 
ATGTACAAGT GGCCAGCCTA CCCTGACTAT ACAGCTGGTG CTGCCTATGT TGTCTCCAGA 
GATGTAGCTG CCAAAATCTA TGAGGCATCG CAGACACTGA ATTCCAGTAT GTACATAGAT 
GATGTATTCA TGGGCCTCTG TGCCAATAAA GTGGGAATCT TGCCACAGGA CCATGTATTT 
TTCTCTGGGG AAGGGAAAAT TCCTTATCAC CCCTGCATCT ATGAAAAGAT GATGACATCT 
CACGGACACT TACAAGATCT GCAGGACCTC TGGATAGAGG CTACACATCC TAAAGTAAAG 
AACATTTCAA AAGGGTTTTT TGGTCAAATA TACTGCAGGT TAATTAAGAT AGTTCTTCTC 
TGCAGACTGA CTTACAGGAA TTCATACCCT TGTTGGGCTG CATTTGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B3gnt5 ( XM_011245803.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244105.1 Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu07444
Clone ID Related Accession (Same CDS sequence)	XM_011245803.1 , NM_001159408.1 , NM_054052.3 , XM_030248916.1 , XM_006521706.3 , XM_006521706.2 , NM_001159407.1
Accession Version	XM_011245803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase isoform X1
Comment

ATGAGACTGT TTGTTAGCAG AAGAGTCAAA AGATGGAAAA TTTTTCACTT TTTTGTCACT 
TGTTTTATAT TAAGCTTCAT GGTTTTTTGG AGCCCAATCA ATAATTACAT CATGAGCCAT 
ATGAAGTCCT ACTCCTACAG ATACCTCGTA AATAGCTATG GCTTTGTAAA CAATTCCCTG 
TCTCTCAAGC ACAGCTCTGT GCAGCCTCAC TACCCATACT TGATCAACCA CAGAGAGAAG 
TGTCAGGCTC AAGATGTCCT CCTCTTACTG TTTATAAAGA CTGCCCCTGA AAACTATGGC 
CGACGTTCTG CAATCAGAAA AACGTGGGGC AATGAGAACT ATGTTCAGTC TCAACTCAAT 
GCCAACATCA AAATTCTGTT TGCATTAGGA ACTCCTGGTC CACTGAAGGG AAAAGAACTG 
CAAAAAAGAC TAATCGGGGA AGATCAAGTG TACAAGGATA TAATTCAGCA AGATTTCATT 
GATTCTTTCC ACAATCTTAC TTCTAAATTC CTTCTTCAGT TCAGCTGGGC AAATACCTTT 
TGTCCACATG CCAAATTCCT GATGACTGCT GATGATGATA TATTTATCCA CATGCCAAAT 
CTCATTGAAT ATCTTCAAGG GCTAGAGCAG ATTGGAGTTC GAGACTTTTG GATTGGTCAC 
GTTCATCGAG GTGGCCCTCC TGTTAGGGAT AAAAGCAGCA AATACTATGT TCCCTATGAA 
ATGTACAAGT GGCCAGCCTA CCCTGACTAT ACAGCTGGTG CTGCCTATGT TGTCTCCAGA 
GATGTAGCTG CCAAAATCTA TGAGGCATCG CAGACACTGA ATTCCAGTAT GTACATAGAT 
GATGTATTCA TGGGCCTCTG TGCCAATAAA GTGGGAATCT TGCCACAGGA CCATGTATTT 
TTCTCTGGGG AAGGGAAAAT TCCTTATCAC CCCTGCATCT ATGAAAAGAT GATGACATCT 
CACGGACACT TACAAGATCT GCAGGACCTC TGGATAGAGG CTACACATCC TAAAGTAAAG 
AACATTTCAA AAGGGTTTTT TGGTCAAATA TACTGCAGGT TAATTAAGAT AGTTCTTCTC 
TGCAGACTGA CTTACAGGAA TTCATACCCT TGTTGGGCTG CATTTGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244105.1
Translation	MRLFVSRRVKRWKIFHFFVTCFILSFMVFWSPINNYIMSHMKSYSYRYLVNSYGFVNNSLSLKHSSVQPHYPYLINHREKCQAQDVLLLLFIKTAPENYGRRSAIRKTWGNENYVQSQLNANIKILFALGTPGPLKGKELQKRLIGEDQVYKDIIQQDFIDSFHNLTSKFLLQFSWANTFCPHAKFLMTADDDIFIHMPNLIEYLQGLEQIGVRDFWIGHVHRGGPPVRDKSSKYYVPYEMYKWPAYPDYTAGAAYVVSRDVAAKIYEASQTLNSSMYIDDVFMGLCANKVGILPQDHVFFSGEGKIPYHPCIYEKMMTSHGHLQDLQDLWIEATHPKVKNISKGFFGQIYCRLIKIVLLCRLTYRNSYPCWAAFA

Target ORF information:

RefSeq Version	XM_011245803.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245803.1

ORF Insert Sequence:

ATGAGACTGT TTGTTAGCAG AAGAGTCAAA AGATGGAAAA TTTTTCACTT TTTTGTCACT 
TGTTTTATAT TAAGCTTCAT GGTTTTTTGG AGCCCAATCA ATAATTACAT CATGAGCCAT 
ATGAAGTCCT ACTCCTACAG ATACCTCGTA AATAGCTATG GCTTTGTAAA CAATTCCCTG 
TCTCTCAAGC ACAGCTCTGT GCAGCCTCAC TACCCATACT TGATCAACCA CAGAGAGAAG 
TGTCAGGCTC AAGATGTCCT CCTCTTACTG TTTATAAAGA CTGCCCCTGA AAACTATGGC 
CGACGTTCTG CAATCAGAAA AACGTGGGGC AATGAGAACT ATGTTCAGTC TCAACTCAAT 
GCCAACATCA AAATTCTGTT TGCATTAGGA ACTCCTGGTC CACTGAAGGG AAAAGAACTG 
CAAAAAAGAC TAATCGGGGA AGATCAAGTG TACAAGGATA TAATTCAGCA AGATTTCATT 
GATTCTTTCC ACAATCTTAC TTCTAAATTC CTTCTTCAGT TCAGCTGGGC AAATACCTTT 
TGTCCACATG CCAAATTCCT GATGACTGCT GATGATGATA TATTTATCCA CATGCCAAAT 
CTCATTGAAT ATCTTCAAGG GCTAGAGCAG ATTGGAGTTC GAGACTTTTG GATTGGTCAC 
GTTCATCGAG GTGGCCCTCC TGTTAGGGAT AAAAGCAGCA AATACTATGT TCCCTATGAA 
ATGTACAAGT GGCCAGCCTA CCCTGACTAT ACAGCTGGTG CTGCCTATGT TGTCTCCAGA 
GATGTAGCTG CCAAAATCTA TGAGGCATCG CAGACACTGA ATTCCAGTAT GTACATAGAT 
GATGTATTCA TGGGCCTCTG TGCCAATAAA GTGGGAATCT TGCCACAGGA CCATGTATTT 
TTCTCTGGGG AAGGGAAAAT TCCTTATCAC CCCTGCATCT ATGAAAAGAT GATGACATCT 
CACGGACACT TACAAGATCT GCAGGACCTC TGGATAGAGG CTACACATCC TAAAGTAAAG 
AACATTTCAA AAGGGTTTTT TGGTCAAATA TACTGCAGGT TAATTAAGAT AGTTCTTCTC 
TGCAGACTGA CTTACAGGAA TTCATACCCT TGTTGGGCTG CATTTGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B3gnt5 ( XM_030248916.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104776.1 Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu07444
Clone ID Related Accession (Same CDS sequence)	XM_011245803.1 , NM_001159408.1 , NM_054052.3 , XM_030248916.1 , XM_006521706.3 , XM_006521706.2 , NM_001159407.1
Accession Version	XM_030248916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACTGT TTGTTAGCAG AAGAGTCAAA AGATGGAAAA TTTTTCACTT TTTTGTCACT 
TGTTTTATAT TAAGCTTCAT GGTTTTTTGG AGCCCAATCA ATAATTACAT CATGAGCCAT 
ATGAAGTCCT ACTCCTACAG ATACCTCGTA AATAGCTATG GCTTTGTAAA CAATTCCCTG 
TCTCTCAAGC ACAGCTCTGT GCAGCCTCAC TACCCATACT TGATCAACCA CAGAGAGAAG 
TGTCAGGCTC AAGATGTCCT CCTCTTACTG TTTATAAAGA CTGCCCCTGA AAACTATGGC 
CGACGTTCTG CAATCAGAAA AACGTGGGGC AATGAGAACT ATGTTCAGTC TCAACTCAAT 
GCCAACATCA AAATTCTGTT TGCATTAGGA ACTCCTGGTC CACTGAAGGG AAAAGAACTG 
CAAAAAAGAC TAATCGGGGA AGATCAAGTG TACAAGGATA TAATTCAGCA AGATTTCATT 
GATTCTTTCC ACAATCTTAC TTCTAAATTC CTTCTTCAGT TCAGCTGGGC AAATACCTTT 
TGTCCACATG CCAAATTCCT GATGACTGCT GATGATGATA TATTTATCCA CATGCCAAAT 
CTCATTGAAT ATCTTCAAGG GCTAGAGCAG ATTGGAGTTC GAGACTTTTG GATTGGTCAC 
GTTCATCGAG GTGGCCCTCC TGTTAGGGAT AAAAGCAGCA AATACTATGT TCCCTATGAA 
ATGTACAAGT GGCCAGCCTA CCCTGACTAT ACAGCTGGTG CTGCCTATGT TGTCTCCAGA 
GATGTAGCTG CCAAAATCTA TGAGGCATCG CAGACACTGA ATTCCAGTAT GTACATAGAT 
GATGTATTCA TGGGCCTCTG TGCCAATAAA GTGGGAATCT TGCCACAGGA CCATGTATTT 
TTCTCTGGGG AAGGGAAAAT TCCTTATCAC CCCTGCATCT ATGAAAAGAT GATGACATCT 
CACGGACACT TACAAGATCT GCAGGACCTC TGGATAGAGG CTACACATCC TAAAGTAAAG 
AACATTTCAA AAGGGTTTTT TGGTCAAATA TACTGCAGGT TAATTAAGAT AGTTCTTCTC 
TGCAGACTGA CTTACAGGAA TTCATACCCT TGTTGGGCTG CATTTGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104776.1
CDS	1073..2203
Translation	MRLFVSRRVKRWKIFHFFVTCFILSFMVFWSPINNYIMSHMKSYSYRYLVNSYGFVNNSLSLKHSSVQPHYPYLINHREKCQAQDVLLLLFIKTAPENYGRRSAIRKTWGNENYVQSQLNANIKILFALGTPGPLKGKELQKRLIGEDQVYKDIIQQDFIDSFHNLTSKFLLQFSWANTFCPHAKFLMTADDDIFIHMPNLIEYLQGLEQIGVRDFWIGHVHRGGPPVRDKSSKYYVPYEMYKWPAYPDYTAGAAYVVSRDVAAKIYEASQTLNSSMYIDDVFMGLCANKVGILPQDHVFFSGEGKIPYHPCIYEKMMTSHGHLQDLQDLWIEATHPKVKNISKGFFGQIYCRLIKIVLLCRLTYRNSYPCWAAFA

Target ORF information:

RefSeq Version	XM_030248916.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248916.1

ORF Insert Sequence:

ATGAGACTGT TTGTTAGCAG AAGAGTCAAA AGATGGAAAA TTTTTCACTT TTTTGTCACT 
TGTTTTATAT TAAGCTTCAT GGTTTTTTGG AGCCCAATCA ATAATTACAT CATGAGCCAT 
ATGAAGTCCT ACTCCTACAG ATACCTCGTA AATAGCTATG GCTTTGTAAA CAATTCCCTG 
TCTCTCAAGC ACAGCTCTGT GCAGCCTCAC TACCCATACT TGATCAACCA CAGAGAGAAG 
TGTCAGGCTC AAGATGTCCT CCTCTTACTG TTTATAAAGA CTGCCCCTGA AAACTATGGC 
CGACGTTCTG CAATCAGAAA AACGTGGGGC AATGAGAACT ATGTTCAGTC TCAACTCAAT 
GCCAACATCA AAATTCTGTT TGCATTAGGA ACTCCTGGTC CACTGAAGGG AAAAGAACTG 
CAAAAAAGAC TAATCGGGGA AGATCAAGTG TACAAGGATA TAATTCAGCA AGATTTCATT 
GATTCTTTCC ACAATCTTAC TTCTAAATTC CTTCTTCAGT TCAGCTGGGC AAATACCTTT 
TGTCCACATG CCAAATTCCT GATGACTGCT GATGATGATA TATTTATCCA CATGCCAAAT 
CTCATTGAAT ATCTTCAAGG GCTAGAGCAG ATTGGAGTTC GAGACTTTTG GATTGGTCAC 
GTTCATCGAG GTGGCCCTCC TGTTAGGGAT AAAAGCAGCA AATACTATGT TCCCTATGAA 
ATGTACAAGT GGCCAGCCTA CCCTGACTAT ACAGCTGGTG CTGCCTATGT TGTCTCCAGA 
GATGTAGCTG CCAAAATCTA TGAGGCATCG CAGACACTGA ATTCCAGTAT GTACATAGAT 
GATGTATTCA TGGGCCTCTG TGCCAATAAA GTGGGAATCT TGCCACAGGA CCATGTATTT 
TTCTCTGGGG AAGGGAAAAT TCCTTATCAC CCCTGCATCT ATGAAAAGAT GATGACATCT 
CACGGACACT TACAAGATCT GCAGGACCTC TGGATAGAGG CTACACATCC TAAAGTAAAG 
AACATTTCAA AAGGGTTTTT TGGTCAAATA TACTGCAGGT TAATTAAGAT AGTTCTTCTC 
TGCAGACTGA CTTACAGGAA TTCATACCCT TGTTGGGCTG CATTTGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B3gnt5 ( XM_006521706.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521769.1 Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu07444
Clone ID Related Accession (Same CDS sequence)	XM_011245803.1 , NM_001159408.1 , NM_054052.3 , XM_030248916.1 , XM_006521706.3 , XM_006521706.2 , NM_001159407.1
Accession Version	XM_006521706.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006521706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACTGT TTGTTAGCAG AAGAGTCAAA AGATGGAAAA TTTTTCACTT TTTTGTCACT 
TGTTTTATAT TAAGCTTCAT GGTTTTTTGG AGCCCAATCA ATAATTACAT CATGAGCCAT 
ATGAAGTCCT ACTCCTACAG ATACCTCGTA AATAGCTATG GCTTTGTAAA CAATTCCCTG 
TCTCTCAAGC ACAGCTCTGT GCAGCCTCAC TACCCATACT TGATCAACCA CAGAGAGAAG 
TGTCAGGCTC AAGATGTCCT CCTCTTACTG TTTATAAAGA CTGCCCCTGA AAACTATGGC 
CGACGTTCTG CAATCAGAAA AACGTGGGGC AATGAGAACT ATGTTCAGTC TCAACTCAAT 
GCCAACATCA AAATTCTGTT TGCATTAGGA ACTCCTGGTC CACTGAAGGG AAAAGAACTG 
CAAAAAAGAC TAATCGGGGA AGATCAAGTG TACAAGGATA TAATTCAGCA AGATTTCATT 
GATTCTTTCC ACAATCTTAC TTCTAAATTC CTTCTTCAGT TCAGCTGGGC AAATACCTTT 
TGTCCACATG CCAAATTCCT GATGACTGCT GATGATGATA TATTTATCCA CATGCCAAAT 
CTCATTGAAT ATCTTCAAGG GCTAGAGCAG ATTGGAGTTC GAGACTTTTG GATTGGTCAC 
GTTCATCGAG GTGGCCCTCC TGTTAGGGAT AAAAGCAGCA AATACTATGT TCCCTATGAA 
ATGTACAAGT GGCCAGCCTA CCCTGACTAT ACAGCTGGTG CTGCCTATGT TGTCTCCAGA 
GATGTAGCTG CCAAAATCTA TGAGGCATCG CAGACACTGA ATTCCAGTAT GTACATAGAT 
GATGTATTCA TGGGCCTCTG TGCCAATAAA GTGGGAATCT TGCCACAGGA CCATGTATTT 
TTCTCTGGGG AAGGGAAAAT TCCTTATCAC CCCTGCATCT ATGAAAAGAT GATGACATCT 
CACGGACACT TACAAGATCT GCAGGACCTC TGGATAGAGG CTACACATCC TAAAGTAAAG 
AACATTTCAA AAGGGTTTTT TGGTCAAATA TACTGCAGGT TAATTAAGAT AGTTCTTCTC 
TGCAGACTGA CTTACAGGAA TTCATACCCT TGTTGGGCTG CATTTGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521769.1
CDS	855..1985
Translation	MRLFVSRRVKRWKIFHFFVTCFILSFMVFWSPINNYIMSHMKSYSYRYLVNSYGFVNNSLSLKHSSVQPHYPYLINHREKCQAQDVLLLLFIKTAPENYGRRSAIRKTWGNENYVQSQLNANIKILFALGTPGPLKGKELQKRLIGEDQVYKDIIQQDFIDSFHNLTSKFLLQFSWANTFCPHAKFLMTADDDIFIHMPNLIEYLQGLEQIGVRDFWIGHVHRGGPPVRDKSSKYYVPYEMYKWPAYPDYTAGAAYVVSRDVAAKIYEASQTLNSSMYIDDVFMGLCANKVGILPQDHVFFSGEGKIPYHPCIYEKMMTSHGHLQDLQDLWIEATHPKVKNISKGFFGQIYCRLIKIVLLCRLTYRNSYPCWAAFA

Target ORF information:

RefSeq Version	XM_006521706.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521706.3

ORF Insert Sequence:

ATGAGACTGT TTGTTAGCAG AAGAGTCAAA AGATGGAAAA TTTTTCACTT TTTTGTCACT 
TGTTTTATAT TAAGCTTCAT GGTTTTTTGG AGCCCAATCA ATAATTACAT CATGAGCCAT 
ATGAAGTCCT ACTCCTACAG ATACCTCGTA AATAGCTATG GCTTTGTAAA CAATTCCCTG 
TCTCTCAAGC ACAGCTCTGT GCAGCCTCAC TACCCATACT TGATCAACCA CAGAGAGAAG 
TGTCAGGCTC AAGATGTCCT CCTCTTACTG TTTATAAAGA CTGCCCCTGA AAACTATGGC 
CGACGTTCTG CAATCAGAAA AACGTGGGGC AATGAGAACT ATGTTCAGTC TCAACTCAAT 
GCCAACATCA AAATTCTGTT TGCATTAGGA ACTCCTGGTC CACTGAAGGG AAAAGAACTG 
CAAAAAAGAC TAATCGGGGA AGATCAAGTG TACAAGGATA TAATTCAGCA AGATTTCATT 
GATTCTTTCC ACAATCTTAC TTCTAAATTC CTTCTTCAGT TCAGCTGGGC AAATACCTTT 
TGTCCACATG CCAAATTCCT GATGACTGCT GATGATGATA TATTTATCCA CATGCCAAAT 
CTCATTGAAT ATCTTCAAGG GCTAGAGCAG ATTGGAGTTC GAGACTTTTG GATTGGTCAC 
GTTCATCGAG GTGGCCCTCC TGTTAGGGAT AAAAGCAGCA AATACTATGT TCCCTATGAA 
ATGTACAAGT GGCCAGCCTA CCCTGACTAT ACAGCTGGTG CTGCCTATGT TGTCTCCAGA 
GATGTAGCTG CCAAAATCTA TGAGGCATCG CAGACACTGA ATTCCAGTAT GTACATAGAT 
GATGTATTCA TGGGCCTCTG TGCCAATAAA GTGGGAATCT TGCCACAGGA CCATGTATTT 
TTCTCTGGGG AAGGGAAAAT TCCTTATCAC CCCTGCATCT ATGAAAAGAT GATGACATCT 
CACGGACACT TACAAGATCT GCAGGACCTC TGGATAGAGG CTACACATCC TAAAGTAAAG 
AACATTTCAA AAGGGTTTTT TGGTCAAATA TACTGCAGGT TAATTAAGAT AGTTCTTCTC 
TGCAGACTGA CTTACAGGAA TTCATACCCT TGTTGGGCTG CATTTGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B3gnt5 ( XM_006521706.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521769.1 Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu07444
Clone ID Related Accession (Same CDS sequence)	XM_011245803.1 , NM_001159408.1 , NM_054052.3 , XM_030248916.1 , XM_006521706.3 , XM_006521706.2 , NM_001159407.1
Accession Version	XM_006521706.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase isoform X1
Comment

ATGAGACTGT TTGTTAGCAG AAGAGTCAAA AGATGGAAAA TTTTTCACTT TTTTGTCACT 
TGTTTTATAT TAAGCTTCAT GGTTTTTTGG AGCCCAATCA ATAATTACAT CATGAGCCAT 
ATGAAGTCCT ACTCCTACAG ATACCTCGTA AATAGCTATG GCTTTGTAAA CAATTCCCTG 
TCTCTCAAGC ACAGCTCTGT GCAGCCTCAC TACCCATACT TGATCAACCA CAGAGAGAAG 
TGTCAGGCTC AAGATGTCCT CCTCTTACTG TTTATAAAGA CTGCCCCTGA AAACTATGGC 
CGACGTTCTG CAATCAGAAA AACGTGGGGC AATGAGAACT ATGTTCAGTC TCAACTCAAT 
GCCAACATCA AAATTCTGTT TGCATTAGGA ACTCCTGGTC CACTGAAGGG AAAAGAACTG 
CAAAAAAGAC TAATCGGGGA AGATCAAGTG TACAAGGATA TAATTCAGCA AGATTTCATT 
GATTCTTTCC ACAATCTTAC TTCTAAATTC CTTCTTCAGT TCAGCTGGGC AAATACCTTT 
TGTCCACATG CCAAATTCCT GATGACTGCT GATGATGATA TATTTATCCA CATGCCAAAT 
CTCATTGAAT ATCTTCAAGG GCTAGAGCAG ATTGGAGTTC GAGACTTTTG GATTGGTCAC 
GTTCATCGAG GTGGCCCTCC TGTTAGGGAT AAAAGCAGCA AATACTATGT TCCCTATGAA 
ATGTACAAGT GGCCAGCCTA CCCTGACTAT ACAGCTGGTG CTGCCTATGT TGTCTCCAGA 
GATGTAGCTG CCAAAATCTA TGAGGCATCG CAGACACTGA ATTCCAGTAT GTACATAGAT 
GATGTATTCA TGGGCCTCTG TGCCAATAAA GTGGGAATCT TGCCACAGGA CCATGTATTT 
TTCTCTGGGG AAGGGAAAAT TCCTTATCAC CCCTGCATCT ATGAAAAGAT GATGACATCT 
CACGGACACT TACAAGATCT GCAGGACCTC TGGATAGAGG CTACACATCC TAAAGTAAAG 
AACATTTCAA AAGGGTTTTT TGGTCAAATA TACTGCAGGT TAATTAAGAT AGTTCTTCTC 
TGCAGACTGA CTTACAGGAA TTCATACCCT TGTTGGGCTG CATTTGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521769.1
Translation	MRLFVSRRVKRWKIFHFFVTCFILSFMVFWSPINNYIMSHMKSYSYRYLVNSYGFVNNSLSLKHSSVQPHYPYLINHREKCQAQDVLLLLFIKTAPENYGRRSAIRKTWGNENYVQSQLNANIKILFALGTPGPLKGKELQKRLIGEDQVYKDIIQQDFIDSFHNLTSKFLLQFSWANTFCPHAKFLMTADDDIFIHMPNLIEYLQGLEQIGVRDFWIGHVHRGGPPVRDKSSKYYVPYEMYKWPAYPDYTAGAAYVVSRDVAAKIYEASQTLNSSMYIDDVFMGLCANKVGILPQDHVFFSGEGKIPYHPCIYEKMMTSHGHLQDLQDLWIEATHPKVKNISKGFFGQIYCRLIKIVLLCRLTYRNSYPCWAAFA

Target ORF information:

RefSeq Version	XM_006521706.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006521706.2

ORF Insert Sequence:

ATGAGACTGT TTGTTAGCAG AAGAGTCAAA AGATGGAAAA TTTTTCACTT TTTTGTCACT 
TGTTTTATAT TAAGCTTCAT GGTTTTTTGG AGCCCAATCA ATAATTACAT CATGAGCCAT 
ATGAAGTCCT ACTCCTACAG ATACCTCGTA AATAGCTATG GCTTTGTAAA CAATTCCCTG 
TCTCTCAAGC ACAGCTCTGT GCAGCCTCAC TACCCATACT TGATCAACCA CAGAGAGAAG 
TGTCAGGCTC AAGATGTCCT CCTCTTACTG TTTATAAAGA CTGCCCCTGA AAACTATGGC 
CGACGTTCTG CAATCAGAAA AACGTGGGGC AATGAGAACT ATGTTCAGTC TCAACTCAAT 
GCCAACATCA AAATTCTGTT TGCATTAGGA ACTCCTGGTC CACTGAAGGG AAAAGAACTG 
CAAAAAAGAC TAATCGGGGA AGATCAAGTG TACAAGGATA TAATTCAGCA AGATTTCATT 
GATTCTTTCC ACAATCTTAC TTCTAAATTC CTTCTTCAGT TCAGCTGGGC AAATACCTTT 
TGTCCACATG CCAAATTCCT GATGACTGCT GATGATGATA TATTTATCCA CATGCCAAAT 
CTCATTGAAT ATCTTCAAGG GCTAGAGCAG ATTGGAGTTC GAGACTTTTG GATTGGTCAC 
GTTCATCGAG GTGGCCCTCC TGTTAGGGAT AAAAGCAGCA AATACTATGT TCCCTATGAA 
ATGTACAAGT GGCCAGCCTA CCCTGACTAT ACAGCTGGTG CTGCCTATGT TGTCTCCAGA 
GATGTAGCTG CCAAAATCTA TGAGGCATCG CAGACACTGA ATTCCAGTAT GTACATAGAT 
GATGTATTCA TGGGCCTCTG TGCCAATAAA GTGGGAATCT TGCCACAGGA CCATGTATTT 
TTCTCTGGGG AAGGGAAAAT TCCTTATCAC CCCTGCATCT ATGAAAAGAT GATGACATCT 
CACGGACACT TACAAGATCT GCAGGACCTC TGGATAGAGG CTACACATCC TAAAGTAAAG 
AACATTTCAA AAGGGTTTTT TGGTCAAATA TACTGCAGGT TAATTAAGAT AGTTCTTCTC 
TGCAGACTGA CTTACAGGAA TTCATACCCT TGTTGGGCTG CATTTGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Multiple phenotypic changes in mice after knockout of the B3gnt5 gene, encoding Lc3 synthase--a key enzyme in lacto-neolacto ganglioside synthesis.
BMC developmental biology10114(2010 Nov)
Kuan CT,Chang J,Mansson JE,Li J,Pegram C,Fredman P,McLendon RE,Bigner DD

Lack of lacto/neolacto-glycolipids enhances the formation of glycolipid-enriched microdomains, facilitating B cell activation.
Proceedings of the National Academy of Sciences of the United States of America107(26)11900-5(2010 Jun)
Togayachi A,Kozono Y,Ikehara Y,Ito H,Suzuki N,Tsunoda Y,Abe S,Sato T,Nakamura K,Suzuki M,Goda HM,Ito M,Kudo T,Takahashi S,Narimatsu H

The Lc3-synthase gene B3gnt5 is essential to pre-implantation development of the murine embryo.
BMC developmental biology8109(2008 Nov)
Biellmann F,H?lsmeier AJ,Zhou D,Cinelli P,Hennet T

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Genome research11(9)1553-8(2001 Sep)
Piao Y,Ko NT,Lim MK,Ko MS

Cloning of a mouse beta 1,3 N-acetylglucosaminyltransferase GlcNAc(beta 1,3)Gal(beta 1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis.
The Journal of biological chemistry276(32)30261-9(2001 Aug)
Henion TR,Zhou D,Wolfer DP,Jungalwala FB,Hennet T

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B3gnt5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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