Chd4 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006505282.1	XP_006505345.1	chromodomain-helicase-DNA-binding protein 4 isoform X4
XM_006505283.1	XP_006505346.1	chromodomain-helicase-DNA-binding protein 4 isoform X5
XM_006505284.1	XP_006505347.1	chromodomain-helicase-DNA-binding protein 4 isoform X6
XM_006505281.1	XP_006505344.1	chromodomain-helicase-DNA-binding protein 4 isoform X1
XM_017321295.1	XP_017176784.1	chromodomain-helicase-DNA-binding protein 4 isoform X11
NM_145979.2	NP_666091.1	chromodomain-helicase-DNA-binding protein 4
XM_006505287.1	XP_006505350.1	chromodomain-helicase-DNA-binding protein 4 isoform X4
XM_006505285.1	XP_006505348.1	chromodomain-helicase-DNA-binding protein 4 isoform X2
XM_006505280.1	XP_006505343.1	chromodomain-helicase-DNA-binding protein 4 isoform X1
XM_017321294.1	XP_017176783.1	chromodomain-helicase-DNA-binding protein 4 isoform X5
XM_017321295.2	XP_017176784.1	chromodomain-helicase-DNA-binding protein 4 isoform X6
NM_001346610.1	NP_001333539.1	chromodomain-helicase-DNA-binding protein 4
XM_017321296.1	XP_017176785.1	chromodomain-helicase-DNA-binding protein 4 isoform X12
XM_030255054.1	XP_030110914.1	chromodomain-helicase-DNA-binding protein 4 isoform X8
XM_017321296.2	XP_017176785.1	chromodomain-helicase-DNA-binding protein 4 isoform X9
XM_017321292.1	XP_017176781.1	chromodomain-helicase-DNA-binding protein 4 isoform X3
XM_006505286.1	XP_006505349.1	chromodomain-helicase-DNA-binding protein 4 isoform X3
XM_030255053.1	XP_030110913.1	chromodomain-helicase-DNA-binding protein 4 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu81597	XM_017321295.2 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu81598	XM_017321296.2 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu89091	XM_030255053.1 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu89092	XM_030255054.1 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu19517	NM_145979.2 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1406.30 $2009.00
OMu19517	NM_001346610.1 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1406.30 $2009.00
OMu36718	XM_006505280.1 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu36719	XM_006505281.1 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu36720	XM_006505282.1 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu36721	XM_006505283.1 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu36722	XM_006505284.1 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu36723	XM_006505285.1 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu36724	XM_006505286.1 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu36725	XM_006505287.1 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu81595	XM_017321292.1 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu81596	XM_017321294.1 Latest version!	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OMu81597	XM_017321295.1	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu81598	XM_017321296.1	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

Chd4 ( XM_017321295.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176784.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu81597
Clone ID Related Accession (Same CDS sequence)	XM_017321295.1 , XM_017321295.2
Accession Version	XM_017321295.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321295.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGCG AGATCATCCT GTGTGATACC 
TGTCCCCGAG CCTACCATAT GGTGTGCCTG GACCCAGACA TGGAGAAGGC CCCGGAGGGC 
AAGTGGAGCT GTCCGCACTG TGAGAAGGAG GGGATCCAGT GGGAAGCTAA GGAGGACAAT 
TCTGAGGGTG AGGAGATTCT GGAAGAAGTC GGGGGGGACC CAGAAGAGGA GGATGACCAT 
CACATGGAAT TCTGTCGCGT CTGCAAGGAC GGCGGGGAGC TCCTGTGCTG TGACACATGC 
CCTTCTTCCT ACCACATCCA CTGCCTGAAC CCCCCGCTGC CAGAGATCCC AAACGGCGAA 
TGGCTCTGTC CCCGCTGTAC TTGTCCAGCT CTTAAGGGCA AAGTGCAGAA GATCCTAATT 
TGGAAGTGGG GCCAGCCACC GTCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGACCCT 
AATACCCCGT CGCCCAAGCC CTTGGAGGGG CGGCCAGAGA GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTGTCAGAAC TGCAGTTGGA GCTGCACTGT 
CAGGTGATGT TCAGAAACTA CCAGCGGAAG AATGATATGG ACGAGCCGCC TTCTGGGGAC 
TTTGGTGGAG ATGAAGAGAA GAGCCGGAAG CGAAAGAACA AGGACCCTAA GTTTGCAGAG 
ATGGAAGAGC GCTTCTACCG CTATGGAATA AAACCGGAGT GGATGATGAT CCACCGCATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTTCACTATT TAATCAAGTG GCGAGACCTG 
CCCTATGACC AGGCGTCCTG GGAGAGCGAG GACGTGGAGA TTCAGGACTA CGACCTGTTC 
AAGCAGAGCT ACTGGAACCA CAGGGAGTTA ATGAGGGGTG AAGAAGGACG ACCCGGCAAG 
AAGCTTAAGA AGGTGAAGCT ACGGAAACTG GAAAGGCCTC CGGAGACCCC AACTGTTGAT 
CCCACCGTGA AGTATGAGCG ACAGCCGGAG TATCTGGATG CGACGGGTGG AACCCTGCAT 
CCCTACCAAA TGGAAGGCTT AAACTGGCTC CGCTTCTCCT GGGCTCAGGG AACCGATACT 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTGCAGA CAGCAGTCTT CCTCTACTCC 
CTCTATAAGG AGGGTCACTC CAAAGGCCCC TTCTTAGTGA GCGCTCCTCT GTCCACCATC 
ATCAACTGGG AGAGAGAGTT TGAGATGTGG GCTCCTGATA TGTATGTGGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGG GAAAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCGAGGATG AAGAAAGAAG CATCGGTGAA ATTCCATGTT 
CTGCTGACAT CCTATGAGTT GATCACCATT GATATGGCCA TCTTGGGTTC TATTGACTGG 
GCCTGCCTCA TTGTGGATGA AGCCCATCGG CTGAAGAACA ACCAGTCTAA GTTCTTTCGA 
GTTTTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTAA CTGGAACTCC GTTACAGAAC 
AATCTAGAGG AACTGTTTCA TCTGCTCAAC TTTCTCACCC CTGAGAGGTT CCACAACTTA 
GAAGGCTTCT TGGAGGAGTT TGCAGACATT GCCAAAGAGG ACCAGATTAA AAAACTCCAC 
GACATGCTGG GGCCTCATAT GTTGCGGCGG CTCAAAGCTG ACGTCTTCAA GAATATGCCA 
TCCAAGACAG AGCTGATTGT GCGCGTGGAG TTGAGTCCTA TGCAGAAGAA GTACTACAAG 
TATATTCTCA CGCGGAATTT CGAAGCACTT AATGCTCGAG GTGGTGGCAA CCAGGTTTCT 
CTGCTGAACG TGGTGATGGA TCTTAAGAAA TGCTGCAACC ACCCTTATCT CTTCCCTGTG 
GCAGCAATGG AAGCCCCTAA GATGCCTAAT GGTATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AGTTGTTGCT GCTGCAGAAG ATGCTTAAGA ACCTGAAAGA GGGAGGGCAT 
CGTGTGCTCA TCTTCTCCCA GATGACCAAG ATGTTGGACT TGCTAGAGGA TTTTTTAGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGTGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGATC GCTTCAATGC ACCGGGTGCT CAACAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGGATCAA TCTGGCCACT GCGGACACAG TTATTATATA TGACTCTGAC 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCCC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TCTACCGGTT TGTGACCCGT GCATCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AGATGATGCT GACACATTTA GTGGTACGGC CTGGCCTGGG CTCCAAGACA 
GGCTCAATGT CTAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAGCTATTC 
AAGGATGAAG CCACGGATGG AGGAGGAGAC AACAAGGAGG GAGAAGACAG CAGTGTTATC 
CACTATGACG ATAAGGCCAT TGAACGACTG CTGGATCGAA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCTCA GTATGTGGTA 
CGGGAAGAAG AGATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATAAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCACAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCCTCAG AGGAAGGTGA TGAAGACTTT GACGAACGGT CAGAAGCTCC CCGCAGGCCC 
AGTCGCAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTAC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCATT TCTTAATGCA 
ATTATGCGAT ATGGCATGCC ACCTCAGGAT GCTTTTACCA CTCAGTGGCT TGTGAGAGAT 
CTTCGAGGCA AGTCAGAGAA AGAATTTAAG GCTTATGTGT CACTCTTCAT GCGACATTTG 
TGTGAGCCTG GGGCAGATGG GGCTGAGACC TTTGCTGATG GCGTCCCACG AGAAGGCCTG 
TCTCGTCAAC ATGTTCTCAC TAGGATCGGT GTCATGTCTC TGATTCGAAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG ACGCTGGAGC ATGCCCGAAC TGGCTGAAGT AGAGGAAAAC 
AAAAAAATGT CTCAGCCAGG TTCACCTTCT CCAAAGACTC CTACACCTTC CACACCAGGG 
GACACACAAC CCAATACTCC TGCGCCTGTG CCACCTGCTG AGGATGGGAT AAAAATAGAG 
GAAAATAGCC TTAAAGAAGA AGAGAGCACA GAAGGAGAGA AGGAAGTTAA ATCTACAGCC 
CCTGAGGCGA CTGTGGAGTG TGCACAGCCC CCTGCCCCTG CCCCTGCCAC TGCCCCTGCC 
ACTGCCACTG CCCCTGAGGA TGACAAGGCT CCTGCTGAGC CTCCTGAGGG GGAGGAAAAA 
GTGGAAAAGG CAGAGGTGAA GGAGAGAACA GAGGAGCCTA TGGAGACAGA GGCCAAAGGT 
ACTACTGAGG TGGAGAAAGT GGAGGAGAAG TCTGCGGTAG ACCTGACCCC GATCGTGGTG 
GAAGACAAAG AAGAGAAGAA GGAGGAGGAG GAGAAAAAGG ACGTGATGCT TCAGAATGGA 
GAAACTCCCA AGGATCTTAG TGATGAGAAG CAGAAGAAAA ACAGCAAACA GCGTTTCATG 
TTCAACATTG CAGATGGTGG TTTTACTGAG TTGCACTCAC TGTGGCAGAA TGAAGAGCGG 
GCAGCCACAG TCACTAAGAA GACTTACGAG ATCTGGCACC GTCGCCATGA TTACTGGCTG 
CTGGCTGGCA TTATAAACCA CGGCTATGCC CGGTGGCAGG ACATCCAGAA TGACCCACGG 
TATGCCATCC TCAATGAGCC TTTCAAGGGT GAAATGAATC GTGGCAATTT TCTAGAGATC 
AAGAATAAAT TTTTAGCTCG AAGATTCAAG CTCTTAGAAC AAGCCCTGGT GATCGAGGAG 
CAGCTTCGCA GGGCAGCTTA CCTGAACATG TCCGAGGACC CCTCTCACCC GTCTATGGCC 
CTTAACACCC GCTTCGCTGA GGTGGAGTGT TTGGCAGAAA GTCACCAGCA CCTGTCCAAG 
GAGTCGATGG CAGGGAATAA GCCGGCCAAT GCAGTCCTAC ACAAAGGTCT CCTGAAACAG 
CTGGAGGAAC TGTTGAGTGA CATGAAAGCT GATGTGACGC GGCTCCCAGC CACCATTGCC 
CGCATCCCCC CAGTCGCTGT GAGGTTACAG ATGTCAGAGC GGAACATTCT CAGCCGCCTG 
GCAAACAGGG CGCCAGAACC TCCCCCACAG CAGGTAGCCC AGCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176784.1
CDS	361..6111
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGLLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017321295.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321295.2

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGCG AGATCATCCT GTGTGATACC 
TGTCCCCGAG CCTACCATAT GGTGTGCCTG GACCCAGACA TGGAGAAGGC CCCGGAGGGC 
AAGTGGAGCT GTCCGCACTG TGAGAAGGAG GGGATCCAGT GGGAAGCTAA GGAGGACAAT 
TCTGAGGGTG AGGAGATTCT GGAAGAAGTC GGGGGGGACC CAGAAGAGGA GGATGACCAT 
CACATGGAAT TCTGTCGCGT CTGCAAGGAC GGCGGGGAGC TCCTGTGCTG TGACACATGC 
CCTTCTTCCT ACCACATCCA CTGCCTGAAC CCCCCGCTGC CAGAGATCCC AAACGGCGAA 
TGGCTCTGTC CCCGCTGTAC TTGTCCAGCT CTTAAGGGCA AAGTGCAGAA GATCCTAATT 
TGGAAGTGGG GCCAGCCACC GTCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGACCCT 
AATACCCCGT CGCCCAAGCC CTTGGAGGGG CGGCCAGAGA GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTGTCAGAAC TGCAGTTGGA GCTGCACTGT 
CAGGTGATGT TCAGAAACTA CCAGCGGAAG AATGATATGG ACGAGCCGCC TTCTGGGGAC 
TTTGGTGGAG ATGAAGAGAA GAGCCGGAAG CGAAAGAACA AGGACCCTAA GTTTGCAGAG 
ATGGAAGAGC GCTTCTACCG CTATGGAATA AAACCGGAGT GGATGATGAT CCACCGCATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTTCACTATT TAATCAAGTG GCGAGACCTG 
CCCTATGACC AGGCGTCCTG GGAGAGCGAG GACGTGGAGA TTCAGGACTA CGACCTGTTC 
AAGCAGAGCT ACTGGAACCA CAGGGAGTTA ATGAGGGGTG AAGAAGGACG ACCCGGCAAG 
AAGCTTAAGA AGGTGAAGCT ACGGAAACTG GAAAGGCCTC CGGAGACCCC AACTGTTGAT 
CCCACCGTGA AGTATGAGCG ACAGCCGGAG TATCTGGATG CGACGGGTGG AACCCTGCAT 
CCCTACCAAA TGGAAGGCTT AAACTGGCTC CGCTTCTCCT GGGCTCAGGG AACCGATACT 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTGCAGA CAGCAGTCTT CCTCTACTCC 
CTCTATAAGG AGGGTCACTC CAAAGGCCCC TTCTTAGTGA GCGCTCCTCT GTCCACCATC 
ATCAACTGGG AGAGAGAGTT TGAGATGTGG GCTCCTGATA TGTATGTGGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGG GAAAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCGAGGATG AAGAAAGAAG CATCGGTGAA ATTCCATGTT 
CTGCTGACAT CCTATGAGTT GATCACCATT GATATGGCCA TCTTGGGTTC TATTGACTGG 
GCCTGCCTCA TTGTGGATGA AGCCCATCGG CTGAAGAACA ACCAGTCTAA GTTCTTTCGA 
GTTTTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTAA CTGGAACTCC GTTACAGAAC 
AATCTAGAGG AACTGTTTCA TCTGCTCAAC TTTCTCACCC CTGAGAGGTT CCACAACTTA 
GAAGGCTTCT TGGAGGAGTT TGCAGACATT GCCAAAGAGG ACCAGATTAA AAAACTCCAC 
GACATGCTGG GGCCTCATAT GTTGCGGCGG CTCAAAGCTG ACGTCTTCAA GAATATGCCA 
TCCAAGACAG AGCTGATTGT GCGCGTGGAG TTGAGTCCTA TGCAGAAGAA GTACTACAAG 
TATATTCTCA CGCGGAATTT CGAAGCACTT AATGCTCGAG GTGGTGGCAA CCAGGTTTCT 
CTGCTGAACG TGGTGATGGA TCTTAAGAAA TGCTGCAACC ACCCTTATCT CTTCCCTGTG 
GCAGCAATGG AAGCCCCTAA GATGCCTAAT GGTATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AGTTGTTGCT GCTGCAGAAG ATGCTTAAGA ACCTGAAAGA GGGAGGGCAT 
CGTGTGCTCA TCTTCTCCCA GATGACCAAG ATGTTGGACT TGCTAGAGGA TTTTTTAGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGTGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGATC GCTTCAATGC ACCGGGTGCT CAACAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGGATCAA TCTGGCCACT GCGGACACAG TTATTATATA TGACTCTGAC 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCCC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TCTACCGGTT TGTGACCCGT GCATCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AGATGATGCT GACACATTTA GTGGTACGGC CTGGCCTGGG CTCCAAGACA 
GGCTCAATGT CTAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAGCTATTC 
AAGGATGAAG CCACGGATGG AGGAGGAGAC AACAAGGAGG GAGAAGACAG CAGTGTTATC 
CACTATGACG ATAAGGCCAT TGAACGACTG CTGGATCGAA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCTCA GTATGTGGTA 
CGGGAAGAAG AGATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATAAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCACAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCCTCAG AGGAAGGTGA TGAAGACTTT GACGAACGGT CAGAAGCTCC CCGCAGGCCC 
AGTCGCAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTAC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCATT TCTTAATGCA 
ATTATGCGAT ATGGCATGCC ACCTCAGGAT GCTTTTACCA CTCAGTGGCT TGTGAGAGAT 
CTTCGAGGCA AGTCAGAGAA AGAATTTAAG GCTTATGTGT CACTCTTCAT GCGACATTTG 
TGTGAGCCTG GGGCAGATGG GGCTGAGACC TTTGCTGATG GCGTCCCACG AGAAGGCCTG 
TCTCGTCAAC ATGTTCTCAC TAGGATCGGT GTCATGTCTC TGATTCGAAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG ACGCTGGAGC ATGCCCGAAC TGGCTGAAGT AGAGGAAAAC 
AAAAAAATGT CTCAGCCAGG TTCACCTTCT CCAAAGACTC CTACACCTTC CACACCAGGG 
GACACACAAC CCAATACTCC TGCGCCTGTG CCACCTGCTG AGGATGGGAT AAAAATAGAG 
GAAAATAGCC TTAAAGAAGA AGAGAGCACA GAAGGAGAGA AGGAAGTTAA ATCTACAGCC 
CCTGAGGCGA CTGTGGAGTG TGCACAGCCC CCTGCCCCTG CCCCTGCCAC TGCCCCTGCC 
ACTGCCACTG CCCCTGAGGA TGACAAGGCT CCTGCTGAGC CTCCTGAGGG GGAGGAAAAA 
GTGGAAAAGG CAGAGGTGAA GGAGAGAACA GAGGAGCCTA TGGAGACAGA GGCCAAAGGT 
ACTACTGAGG TGGAGAAAGT GGAGGAGAAG TCTGCGGTAG ACCTGACCCC GATCGTGGTG 
GAAGACAAAG AAGAGAAGAA GGAGGAGGAG GAGAAAAAGG ACGTGATGCT TCAGAATGGA 
GAAACTCCCA AGGATCTTAG TGATGAGAAG CAGAAGAAAA ACAGCAAACA GCGTTTCATG 
TTCAACATTG CAGATGGTGG TTTTACTGAG TTGCACTCAC TGTGGCAGAA TGAAGAGCGG 
GCAGCCACAG TCACTAAGAA GACTTACGAG ATCTGGCACC GTCGCCATGA TTACTGGCTG 
CTGGCTGGCA TTATAAACCA CGGCTATGCC CGGTGGCAGG ACATCCAGAA TGACCCACGG 
TATGCCATCC TCAATGAGCC TTTCAAGGGT GAAATGAATC GTGGCAATTT TCTAGAGATC 
AAGAATAAAT TTTTAGCTCG AAGATTCAAG CTCTTAGAAC AAGCCCTGGT GATCGAGGAG 
CAGCTTCGCA GGGCAGCTTA CCTGAACATG TCCGAGGACC CCTCTCACCC GTCTATGGCC 
CTTAACACCC GCTTCGCTGA GGTGGAGTGT TTGGCAGAAA GTCACCAGCA CCTGTCCAAG 
GAGTCGATGG CAGGGAATAA GCCGGCCAAT GCAGTCCTAC ACAAAGGTCT CCTGAAACAG 
CTGGAGGAAC TGTTGAGTGA CATGAAAGCT GATGTGACGC GGCTCCCAGC CACCATTGCC 
CGCATCCCCC CAGTCGCTGT GAGGTTACAG ATGTCAGAGC GGAACATTCT CAGCCGCCTG 
GCAAACAGGG CGCCAGAACC TCCCCCACAG CAGGTAGCCC AGCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_017321296.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176785.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X9, mRNA.

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CloneID	OMu81598
Clone ID Related Accession (Same CDS sequence)	XM_017321296.1 , XM_017321296.2
Accession Version	XM_017321296.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321296.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGG 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTA 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCAT TTCTTAATGC AATTATGCGA TATGGCATGC CACCTCAGGA TGCTTTTACC 
ACTCAGTGGC TTGTGAGAGA TCTTCGAGGC AAGTCAGAGA AAGAATTTAA GGCTTATGTG 
TCACTCTTCA TGCGACATTT GTGTGAGCCT GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGCGTCCCAC GAGAAGGCCT GTCTCGTCAA CATGTTCTCA CTAGGATCGG TGTCATGTCT 
CTGATTCGAA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GACGCTGGAG CATGCCCGAA 
CTGGCTGAAG TAGAGGAAAA CAAAAAAATG TCTCAGCCAG GTTCACCTTC TCCAAAGACT 
CCTACACCTT CCACACCAGG GGACACACAA CCCAATACTC CTGCGCCTGT GCCACCTGCT 
GAGGATGGGA TAAAAATAGA GGAAAATAGC CTTAAAGAAG AAGAGAGCAC AGAAGGAGAG 
AAGGAAGTTA AATCTACAGC CCCTGAGGCG ACTGTGGAGT GTGCACAGCC CCCTGCCCCT 
GCCCCTGCCA CTGCCCCTGC CACTGCCACT GCCCCTGAGG ATGACAAGGC TCCTGCTGAG 
CCTCCTGAGG GGGAGGAAAA AGTGGAAAAG GCAGAGGTGA AGGAGAGAAC AGAGGAGCCT 
ATGGAGACAG AGGCCAAAGG TACTACTGAG GTGGAGAAAG TGGAGGAGAA GTCTGCGGTA 
GACCTGACCC CGATCGTGGT GGAAGACAAA GAAGAGAAGA AGGAGGAGGA GGAGAAAAAG 
GACGTGATGC TTCAGAATGG AGAAACTCCC AAGGATCTTA GTGATGAGAA GCAGAAGAAA 
AACAGCAAAC AGCGTTTCAT GTTCAACATT GCAGATGGTG GTTTTACTGA GTTGCACTCA 
CTGTGGCAGA ATGAAGAGCG GGCAGCCACA GTCACTAAGA AGACTTACGA GATCTGGCAC 
CGTCGCCATG ATTACTGGCT GCTGGCTGGC ATTATAAACC ACGGCTATGC CCGGTGGCAG 
GACATCCAGA ATGACCCACG GTATGCCATC CTCAATGAGC CTTTCAAGGG TGAAATGAAT 
CGTGGCAATT TTCTAGAGAT CAAGAATAAA TTTTTAGCTC GAAGATTCAA GCTCTTAGAA 
CAAGCCCTGG TGATCGAGGA GCAGCTTCGC AGGGCAGCTT ACCTGAACAT GTCCGAGGAC 
CCCTCTCACC CGTCTATGGC CCTTAACACC CGCTTCGCTG AGGTGGAGTG TTTGGCAGAA 
AGTCACCAGC ACCTGTCCAA GGAGTCGATG GCAGGGAATA AGCCGGCCAA TGCAGTCCTA 
CACAAAGTCC TGAAACAGCT GGAGGAACTG TTGAGTGACA TGAAAGCTGA TGTGACGCGG 
CTCCCAGCCA CCATTGCCCG CATCCCCCCA GTCGCTGTGA GGTTACAGAT GTCAGAGCGG 
AACATTCTCA GCCGCCTGGC AAACAGGGCG CCAGAACCTC CCCCACAGCA GGTAGCCCAG 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176785.1
CDS	361..6069
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017321296.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321296.2

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGG 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTA 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCAT TTCTTAATGC AATTATGCGA TATGGCATGC CACCTCAGGA TGCTTTTACC 
ACTCAGTGGC TTGTGAGAGA TCTTCGAGGC AAGTCAGAGA AAGAATTTAA GGCTTATGTG 
TCACTCTTCA TGCGACATTT GTGTGAGCCT GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGCGTCCCAC GAGAAGGCCT GTCTCGTCAA CATGTTCTCA CTAGGATCGG TGTCATGTCT 
CTGATTCGAA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GACGCTGGAG CATGCCCGAA 
CTGGCTGAAG TAGAGGAAAA CAAAAAAATG TCTCAGCCAG GTTCACCTTC TCCAAAGACT 
CCTACACCTT CCACACCAGG GGACACACAA CCCAATACTC CTGCGCCTGT GCCACCTGCT 
GAGGATGGGA TAAAAATAGA GGAAAATAGC CTTAAAGAAG AAGAGAGCAC AGAAGGAGAG 
AAGGAAGTTA AATCTACAGC CCCTGAGGCG ACTGTGGAGT GTGCACAGCC CCCTGCCCCT 
GCCCCTGCCA CTGCCCCTGC CACTGCCACT GCCCCTGAGG ATGACAAGGC TCCTGCTGAG 
CCTCCTGAGG GGGAGGAAAA AGTGGAAAAG GCAGAGGTGA AGGAGAGAAC AGAGGAGCCT 
ATGGAGACAG AGGCCAAAGG TACTACTGAG GTGGAGAAAG TGGAGGAGAA GTCTGCGGTA 
GACCTGACCC CGATCGTGGT GGAAGACAAA GAAGAGAAGA AGGAGGAGGA GGAGAAAAAG 
GACGTGATGC TTCAGAATGG AGAAACTCCC AAGGATCTTA GTGATGAGAA GCAGAAGAAA 
AACAGCAAAC AGCGTTTCAT GTTCAACATT GCAGATGGTG GTTTTACTGA GTTGCACTCA 
CTGTGGCAGA ATGAAGAGCG GGCAGCCACA GTCACTAAGA AGACTTACGA GATCTGGCAC 
CGTCGCCATG ATTACTGGCT GCTGGCTGGC ATTATAAACC ACGGCTATGC CCGGTGGCAG 
GACATCCAGA ATGACCCACG GTATGCCATC CTCAATGAGC CTTTCAAGGG TGAAATGAAT 
CGTGGCAATT TTCTAGAGAT CAAGAATAAA TTTTTAGCTC GAAGATTCAA GCTCTTAGAA 
CAAGCCCTGG TGATCGAGGA GCAGCTTCGC AGGGCAGCTT ACCTGAACAT GTCCGAGGAC 
CCCTCTCACC CGTCTATGGC CCTTAACACC CGCTTCGCTG AGGTGGAGTG TTTGGCAGAA 
AGTCACCAGC ACCTGTCCAA GGAGTCGATG GCAGGGAATA AGCCGGCCAA TGCAGTCCTA 
CACAAAGTCC TGAAACAGCT GGAGGAACTG TTGAGTGACA TGAAAGCTGA TGTGACGCGG 
CTCCCAGCCA CCATTGCCCG CATCCCCCCA GTCGCTGTGA GGTTACAGAT GTCAGAGCGG 
AACATTCTCA GCCGCCTGGC AAACAGGGCG CCAGAACCTC CCCCACAGCA GGTAGCCCAG 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_030255053.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110913.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X7, mRNA.

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CloneID	OMu89091
Clone ID Related Accession (Same CDS sequence)	XM_030255053.1
Accession Version	XM_030255053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG CGAGATCATC CTGTGTGATA CCTGTCCCCG AGCCTACCAT 
ATGGTGTGCC TGGACCCAGA CATGGAGAAG GCCCCGGAGG GCAAGTGGAG CTGTCCGCAC 
TGTGAGAAGG AGGGGATCCA GTGGGAAGCT AAGGAGGACA ATTCTGAGGG TGAGGAGATT 
CTGGAAGAAG TCGGGGGGGA CCCAGAAGAG GAGGATGACC ATCACATGGA ATTCTGTCGC 
GTCTGCAAGG ACGGCGGGGA GCTCCTGTGC TGTGACACAT GCCCTTCTTC CTACCACATC 
CACTGCCTGA ACCCCCCGCT GCCAGAGATC CCAAACGGCG AATGGCTCTG TCCCCGCTGT 
ACTTGTCCAG CTCTTAAGGG CAAAGTGCAG AAGATCCTAA TTTGGAAGTG GGGCCAGCCA 
CCGTCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGACC CTAATACCCC GTCGCCCAAG 
CCCTTGGAGG GGCGGCCAGA GAGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTGTCAGA ACTGCAGTTG GAGCTGCACT GTCAGGTGAT GTTCAGAAAC 
TACCAGCGGA AGAATGATAT GGACGAGCCG CCTTCTGGGG ACTTTGGTGG AGATGAAGAG 
AAGAGCCGGA AGCGAAAGAA CAAGGACCCT AAGTTTGCAG AGATGGAAGA GCGCTTCTAC 
CGCTATGGAA TAAAACCGGA GTGGATGATG ATCCACCGCA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTTCACTA TTTAATCAAG TGGCGAGACC TGCCCTATGA CCAGGCGTCC 
TGGGAGAGCG AGGACGTGGA GATTCAGGAC TACGACCTGT TCAAGCAGAG CTACTGGAAC 
CACAGGGAGT TAATGAGGGG TGAAGAAGGA CGACCCGGCA AGAAGCTTAA GAAGGTGAAG 
CTACGGAAAC TGGAAAGGCC TCCGGAGACC CCAACTGTTG ATCCCACCGT GAAGTATGAG 
CGACAGCCGG AGTATCTGGA TGCGACGGGT GGAACCCTGC ATCCCTACCA AATGGAAGGC 
TTAAACTGGC TCCGCTTCTC CTGGGCTCAG GGAACCGATA CTATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTGCA GACAGCAGTC TTCCTCTACT CCCTCTATAA GGAGGGTCAC 
TCCAAAGGCC CCTTCTTAGT GAGCGCTCCT CTGTCCACCA TCATCAACTG GGAGAGAGAG 
TTTGAGATGT GGGCTCCTGA TATGTATGTG GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GGGAAAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCGAGGA TGAAGAAAGA AGCATCGGTG AAATTCCATG TTCTGCTGAC ATCCTATGAG 
TTGATCACCA TTGATATGGC CATCTTGGGT TCTATTGACT GGGCCTGCCT CATTGTGGAT 
GAAGCCCATC GGCTGAAGAA CAACCAGTCT AAGTTCTTTC GAGTTTTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT AACTGGAACT CCGTTACAGA ACAATCTAGA GGAACTGTTT 
CATCTGCTCA ACTTTCTCAC CCCTGAGAGG TTCCACAACT TAGAAGGCTT CTTGGAGGAG 
TTTGCAGACA TTGCCAAAGA GGACCAGATT AAAAAACTCC ACGACATGCT GGGGCCTCAT 
ATGTTGCGGC GGCTCAAAGC TGACGTCTTC AAGAATATGC CATCCAAGAC AGAGCTGATT 
GTGCGCGTGG AGTTGAGTCC TATGCAGAAG AAGTACTACA AGTATATTCT CACGCGGAAT 
TTCGAAGCAC TTAATGCTCG AGGTGGTGGC AACCAGGTTT CTCTGCTGAA CGTGGTGATG 
GATCTTAAGA AATGCTGCAA CCACCCTTAT CTCTTCCCTG TGGCAGCAAT GGAAGCCCCT 
AAGATGCCTA ATGGTATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAGTTGTTG 
CTGCTGCAGA AGATGCTTAA GAACCTGAAA GAGGGAGGGC ATCGTGTGCT CATCTTCTCC 
CAGATGACCA AGATGTTGGA CTTGCTAGAG GATTTTTTAG AACATGAAGG TTATAAATAT 
GAACGTATTG ATGGTGGAAT CACTGGGAAC ATGCGTCAGG AGGCCATTGA TCGCTTCAAT 
GCACCGGGTG CTCAACAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGGATC 
AATCTGGCCA CTGCGGACAC AGTTATTATA TATGACTCTG ACTGGAACCC CCATAATGAC 
ATCCAGGCCT TTAGCAGAGC CCACCGTATT GGGCAAAATA AGAAGGTGAT GATCTACCGG 
TTTGTGACCC GTGCATCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAGATGATG 
CTGACACATT TAGTGGTACG GCCTGGCCTG GGCTCCAAGA CAGGCTCAAT GTCTAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAGCTAT TCAAGGATGA AGCCACGGAT 
GGAGGAGGAG ACAACAAGGA GGGAGAAGAC AGCAGTGTTA TCCACTATGA CGATAAGGCC 
ATTGAACGAC TGCTGGATCG AAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCT CAGTATGTGG TACGGGAAGA AGAGATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATA AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCACA GGAGGACCGA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTACT CAGTGGCCTC AGAGGAAGGT 
GATGAAGACT TTGACGAACG GTCAGAAGCT CCCCGCAGGC CCAGTCGCAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT ACCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTA 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCA TTTCTTAATG CAATTATGCG ATATGGCATG 
CCACCTCAGG ATGCTTTTAC CACTCAGTGG CTTGTGAGAG ATCTTCGAGG CAAGTCAGAG 
AAAGAATTTA AGGCTTATGT GTCACTCTTC ATGCGACATT TGTGTGAGCC TGGGGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGCGTCCCA CGAGAAGGCC TGTCTCGTCA ACATGTTCTC 
ACTAGGATCG GTGTCATGTC TCTGATTCGA AAGAAGGTTC AGGAGTTTGA ACATGTTAAT 
GGACGCTGGA GCATGCCCGA ACTGGCTGAA GTAGAGGAAA ACAAAAAAAT GTCTCAGCCA 
GGTTCACCTT CTCCAAAGAC TCCTACACCT TCCACACCAG GGGACACACA ACCCAATACT 
CCTGCGCCTG TGCCACCTGC TGAGGATGGG ATAAAAATAG AGGAAAATAG CCTTAAAGAA 
GAAGAGAGCA CAGAAGGAGA GAAGGAAGTT AAATCTACAG CCCCTGAGGC GACTGTGGAG 
TGTGCACAGC CCCCTGCCCC TGCCCCTGCC ACTGCCCCTG CCACTGCCAC TGCCCCTGAG 
GATGACAAGG CTCCTGCTGA GCCTCCTGAG GGGGAGGAAA AAGTGGAAAA GGCAGAGGTG 
AAGGAGAGAA CAGAGGAGCC TATGGAGACA GAGGCCAAAG GTACTACTGA GGTGGAGAAA 
GTGGAGGAGA AGTCTGCGGT AGACCTGACC CCGATCGTGG TGGAAGACAA AGAAGAGAAG 
AAGGAGGAGG AGGAGAAAAA GGACGTGATG CTTCAGAATG GAGAAACTCC CAAGGATCTT 
AGTGATGAGA AGCAGAAGAA AAACAGCAAA CAGCGTTTCA TGTTCAACAT TGCAGATGGT 
GGTTTTACTG AGTTGCACTC ACTGTGGCAG AATGAAGAGC GGGCAGCCAC AGTCACTAAG 
AAGACTTACG AGATCTGGCA CCGTCGCCAT GATTACTGGC TGCTGGCTGG CATTATAAAC 
CACGGCTATG CCCGGTGGCA GGACATCCAG AATGACCCAC GGTATGCCAT CCTCAATGAG 
CCTTTCAAGG GTGAAATGAA TCGTGGCAAT TTTCTAGAGA TCAAGAATAA ATTTTTAGCT 
CGAAGATTCA AGCTCTTAGA ACAAGCCCTG GTGATCGAGG AGCAGCTTCG CAGGGCAGCT 
TACCTGAACA TGTCCGAGGA CCCCTCTCAC CCGTCTATGG CCCTTAACAC CCGCTTCGCT 
GAGGTGGAGT GTTTGGCAGA AAGTCACCAG CACCTGTCCA AGGAGTCGAT GGCAGGGAAT 
AAGCCGGCCA ATGCAGTCCT ACACAAAGGT CTCCTGAAAC AGCTGGAGGA ACTGTTGAGT 
GACATGAAAG CTGATGTGAC GCGGCTCCCA GCCACCATTG CCCGCATCCC CCCAGTCGCT 
GTGAGGTTAC AGATGTCAGA GCGGAACATT CTCAGCCGCC TGGCAAACAG GGCGCCAGAA 
CCTCCCCCAC AGCAGGTAGC CCAGCAGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110913.1
CDS	355..6087
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGLLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_030255053.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255053.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG CGAGATCATC CTGTGTGATA CCTGTCCCCG AGCCTACCAT 
ATGGTGTGCC TGGACCCAGA CATGGAGAAG GCCCCGGAGG GCAAGTGGAG CTGTCCGCAC 
TGTGAGAAGG AGGGGATCCA GTGGGAAGCT AAGGAGGACA ATTCTGAGGG TGAGGAGATT 
CTGGAAGAAG TCGGGGGGGA CCCAGAAGAG GAGGATGACC ATCACATGGA ATTCTGTCGC 
GTCTGCAAGG ACGGCGGGGA GCTCCTGTGC TGTGACACAT GCCCTTCTTC CTACCACATC 
CACTGCCTGA ACCCCCCGCT GCCAGAGATC CCAAACGGCG AATGGCTCTG TCCCCGCTGT 
ACTTGTCCAG CTCTTAAGGG CAAAGTGCAG AAGATCCTAA TTTGGAAGTG GGGCCAGCCA 
CCGTCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGACC CTAATACCCC GTCGCCCAAG 
CCCTTGGAGG GGCGGCCAGA GAGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTGTCAGA ACTGCAGTTG GAGCTGCACT GTCAGGTGAT GTTCAGAAAC 
TACCAGCGGA AGAATGATAT GGACGAGCCG CCTTCTGGGG ACTTTGGTGG AGATGAAGAG 
AAGAGCCGGA AGCGAAAGAA CAAGGACCCT AAGTTTGCAG AGATGGAAGA GCGCTTCTAC 
CGCTATGGAA TAAAACCGGA GTGGATGATG ATCCACCGCA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTTCACTA TTTAATCAAG TGGCGAGACC TGCCCTATGA CCAGGCGTCC 
TGGGAGAGCG AGGACGTGGA GATTCAGGAC TACGACCTGT TCAAGCAGAG CTACTGGAAC 
CACAGGGAGT TAATGAGGGG TGAAGAAGGA CGACCCGGCA AGAAGCTTAA GAAGGTGAAG 
CTACGGAAAC TGGAAAGGCC TCCGGAGACC CCAACTGTTG ATCCCACCGT GAAGTATGAG 
CGACAGCCGG AGTATCTGGA TGCGACGGGT GGAACCCTGC ATCCCTACCA AATGGAAGGC 
TTAAACTGGC TCCGCTTCTC CTGGGCTCAG GGAACCGATA CTATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTGCA GACAGCAGTC TTCCTCTACT CCCTCTATAA GGAGGGTCAC 
TCCAAAGGCC CCTTCTTAGT GAGCGCTCCT CTGTCCACCA TCATCAACTG GGAGAGAGAG 
TTTGAGATGT GGGCTCCTGA TATGTATGTG GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GGGAAAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCGAGGA TGAAGAAAGA AGCATCGGTG AAATTCCATG TTCTGCTGAC ATCCTATGAG 
TTGATCACCA TTGATATGGC CATCTTGGGT TCTATTGACT GGGCCTGCCT CATTGTGGAT 
GAAGCCCATC GGCTGAAGAA CAACCAGTCT AAGTTCTTTC GAGTTTTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT AACTGGAACT CCGTTACAGA ACAATCTAGA GGAACTGTTT 
CATCTGCTCA ACTTTCTCAC CCCTGAGAGG TTCCACAACT TAGAAGGCTT CTTGGAGGAG 
TTTGCAGACA TTGCCAAAGA GGACCAGATT AAAAAACTCC ACGACATGCT GGGGCCTCAT 
ATGTTGCGGC GGCTCAAAGC TGACGTCTTC AAGAATATGC CATCCAAGAC AGAGCTGATT 
GTGCGCGTGG AGTTGAGTCC TATGCAGAAG AAGTACTACA AGTATATTCT CACGCGGAAT 
TTCGAAGCAC TTAATGCTCG AGGTGGTGGC AACCAGGTTT CTCTGCTGAA CGTGGTGATG 
GATCTTAAGA AATGCTGCAA CCACCCTTAT CTCTTCCCTG TGGCAGCAAT GGAAGCCCCT 
AAGATGCCTA ATGGTATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAGTTGTTG 
CTGCTGCAGA AGATGCTTAA GAACCTGAAA GAGGGAGGGC ATCGTGTGCT CATCTTCTCC 
CAGATGACCA AGATGTTGGA CTTGCTAGAG GATTTTTTAG AACATGAAGG TTATAAATAT 
GAACGTATTG ATGGTGGAAT CACTGGGAAC ATGCGTCAGG AGGCCATTGA TCGCTTCAAT 
GCACCGGGTG CTCAACAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGGATC 
AATCTGGCCA CTGCGGACAC AGTTATTATA TATGACTCTG ACTGGAACCC CCATAATGAC 
ATCCAGGCCT TTAGCAGAGC CCACCGTATT GGGCAAAATA AGAAGGTGAT GATCTACCGG 
TTTGTGACCC GTGCATCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAGATGATG 
CTGACACATT TAGTGGTACG GCCTGGCCTG GGCTCCAAGA CAGGCTCAAT GTCTAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAGCTAT TCAAGGATGA AGCCACGGAT 
GGAGGAGGAG ACAACAAGGA GGGAGAAGAC AGCAGTGTTA TCCACTATGA CGATAAGGCC 
ATTGAACGAC TGCTGGATCG AAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCT CAGTATGTGG TACGGGAAGA AGAGATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATA AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCACA GGAGGACCGA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTACT CAGTGGCCTC AGAGGAAGGT 
GATGAAGACT TTGACGAACG GTCAGAAGCT CCCCGCAGGC CCAGTCGCAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT ACCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTA 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCA TTTCTTAATG CAATTATGCG ATATGGCATG 
CCACCTCAGG ATGCTTTTAC CACTCAGTGG CTTGTGAGAG ATCTTCGAGG CAAGTCAGAG 
AAAGAATTTA AGGCTTATGT GTCACTCTTC ATGCGACATT TGTGTGAGCC TGGGGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGCGTCCCA CGAGAAGGCC TGTCTCGTCA ACATGTTCTC 
ACTAGGATCG GTGTCATGTC TCTGATTCGA AAGAAGGTTC AGGAGTTTGA ACATGTTAAT 
GGACGCTGGA GCATGCCCGA ACTGGCTGAA GTAGAGGAAA ACAAAAAAAT GTCTCAGCCA 
GGTTCACCTT CTCCAAAGAC TCCTACACCT TCCACACCAG GGGACACACA ACCCAATACT 
CCTGCGCCTG TGCCACCTGC TGAGGATGGG ATAAAAATAG AGGAAAATAG CCTTAAAGAA 
GAAGAGAGCA CAGAAGGAGA GAAGGAAGTT AAATCTACAG CCCCTGAGGC GACTGTGGAG 
TGTGCACAGC CCCCTGCCCC TGCCCCTGCC ACTGCCCCTG CCACTGCCAC TGCCCCTGAG 
GATGACAAGG CTCCTGCTGA GCCTCCTGAG GGGGAGGAAA AAGTGGAAAA GGCAGAGGTG 
AAGGAGAGAA CAGAGGAGCC TATGGAGACA GAGGCCAAAG GTACTACTGA GGTGGAGAAA 
GTGGAGGAGA AGTCTGCGGT AGACCTGACC CCGATCGTGG TGGAAGACAA AGAAGAGAAG 
AAGGAGGAGG AGGAGAAAAA GGACGTGATG CTTCAGAATG GAGAAACTCC CAAGGATCTT 
AGTGATGAGA AGCAGAAGAA AAACAGCAAA CAGCGTTTCA TGTTCAACAT TGCAGATGGT 
GGTTTTACTG AGTTGCACTC ACTGTGGCAG AATGAAGAGC GGGCAGCCAC AGTCACTAAG 
AAGACTTACG AGATCTGGCA CCGTCGCCAT GATTACTGGC TGCTGGCTGG CATTATAAAC 
CACGGCTATG CCCGGTGGCA GGACATCCAG AATGACCCAC GGTATGCCAT CCTCAATGAG 
CCTTTCAAGG GTGAAATGAA TCGTGGCAAT TTTCTAGAGA TCAAGAATAA ATTTTTAGCT 
CGAAGATTCA AGCTCTTAGA ACAAGCCCTG GTGATCGAGG AGCAGCTTCG CAGGGCAGCT 
TACCTGAACA TGTCCGAGGA CCCCTCTCAC CCGTCTATGG CCCTTAACAC CCGCTTCGCT 
GAGGTGGAGT GTTTGGCAGA AAGTCACCAG CACCTGTCCA AGGAGTCGAT GGCAGGGAAT 
AAGCCGGCCA ATGCAGTCCT ACACAAAGGT CTCCTGAAAC AGCTGGAGGA ACTGTTGAGT 
GACATGAAAG CTGATGTGAC GCGGCTCCCA GCCACCATTG CCCGCATCCC CCCAGTCGCT 
GTGAGGTTAC AGATGTCAGA GCGGAACATT CTCAGCCGCC TGGCAAACAG GGCGCCAGAA 
CCTCCCCCAC AGCAGGTAGC CCAGCAGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_030255054.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110914.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X8, mRNA.

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CloneID	OMu89092
Clone ID Related Accession (Same CDS sequence)	XM_030255054.1
Accession Version	XM_030255054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGG 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTA 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCAT TTCTTAATGC AATTATGCGA TATGGCATGC CACCTCAGGA TGCTTTTACC 
ACTCAGTGGC TTGTGAGAGA TCTTCGAGGC AAGTCAGAGA AAGAATTTAA GGCTTATGTG 
TCACTCTTCA TGCGACATTT GTGTGAGCCT GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGCGTCCCAC GAGAAGGCCT GTCTCGTCAA CATGTTCTCA CTAGGATCGG TGTCATGTCT 
CTGATTCGAA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GACGCTGGAG CATGCCCGAA 
CTGGCTGAAG TAGAGGAAAA CAAAAAAATG TCTCAGCCAG GTTCACCTTC TCCAAAGACT 
CCTACACCTT CCACACCAGG GGACACACAA CCCAATACTC CTGCGCCTGT GCCACCTGCT 
GAGGATGGGA TAAAAATAGA GGAAAATAGC CTTAAAGAAG AAGAGAGCAC AGAAGGAGAG 
AAGGAAGTTA AATCTACAGC CCCTGAGGCG ACTGTGGAGT GTGCACAGCC CCCTGCCCCT 
GCCCCTGCCA CTGCCCCTGC CACTGCCACT GCCCCTGAGG ATGACAAGGC TCCTGCTGAG 
CCTCCTGAGG GGGAGGAAAA AGTGGAAAAG GCAGAGGTGA AGGAGAGAAC AGAGGAGCCT 
ATGGAGACAG AGGCCAAAGG TACTACTGAG GTGGAGAAAG TGGAGGAGAA GTCTGCGGTA 
GACCTGACCC CGATCGTGGT GGAAGACAAA GAAGAGAAGA AGGAGGAGGA GGAGAAAAAG 
GACGTGATGC TTCAGAATGG AGAAACTCCC AAGGATCTTA GTGATGAGAA GCAGAAGAAA 
AACAGCAAAC AGCGTTTCAT GTTCAACATT GCAGATGGTG GTTTTACTGA GTTGCACTCA 
CTGTGGCAGA ATGAAGAGCG GGCAGCCACA GTCACTAAGA AGACTTACGA GATCTGGCAC 
CGTCGCCATG ATTACTGGCT GCTGGCTGGC ATTATAAACC ACGGCTATGC CCGGTGGCAG 
GACATCCAGA ATGACCCACG GTATGCCATC CTCAATGAGC CTTTCAAGGG TGAAATGAAT 
CGTGGCAATT TTCTAGAGAT CAAGAATAAA TTTTTAGCTC GAAGATTCAA GCTCTTAGAA 
CAAGCCCTGG TGATCGAGGA GCAGCTTCGC AGGGCAGCTT ACCTGAACAT GTCCGAGGAC 
CCCTCTCACC CGTCTATGGC CCTTAACACC CGCTTCGCTG AGGTGGAGTG TTTGGCAGAA 
AGTCACCAGC ACCTGTCCAA GGAGTCGATG GCAGGGAATA AGCCGGCCAA TGCAGTCCTA 
CACAAAGGTC TCCTGAAACA GCTGGAGGAA CTGTTGAGTG ACATGAAAGC TGATGTGACG 
CGGCTCCCAG CCACCATTGC CCGCATCCCC CCAGTCGCTG TGAGGTTACA GATGTCAGAG 
CGGAACATTC TCAGCCGCCT GGCAAACAGG GCGCCAGAAC CTCCCCCACA GCAGGTAGCC 
CAGCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110914.1
CDS	361..6072
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGLLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_030255054.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255054.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGG 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTA 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCAT TTCTTAATGC AATTATGCGA TATGGCATGC CACCTCAGGA TGCTTTTACC 
ACTCAGTGGC TTGTGAGAGA TCTTCGAGGC AAGTCAGAGA AAGAATTTAA GGCTTATGTG 
TCACTCTTCA TGCGACATTT GTGTGAGCCT GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGCGTCCCAC GAGAAGGCCT GTCTCGTCAA CATGTTCTCA CTAGGATCGG TGTCATGTCT 
CTGATTCGAA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GACGCTGGAG CATGCCCGAA 
CTGGCTGAAG TAGAGGAAAA CAAAAAAATG TCTCAGCCAG GTTCACCTTC TCCAAAGACT 
CCTACACCTT CCACACCAGG GGACACACAA CCCAATACTC CTGCGCCTGT GCCACCTGCT 
GAGGATGGGA TAAAAATAGA GGAAAATAGC CTTAAAGAAG AAGAGAGCAC AGAAGGAGAG 
AAGGAAGTTA AATCTACAGC CCCTGAGGCG ACTGTGGAGT GTGCACAGCC CCCTGCCCCT 
GCCCCTGCCA CTGCCCCTGC CACTGCCACT GCCCCTGAGG ATGACAAGGC TCCTGCTGAG 
CCTCCTGAGG GGGAGGAAAA AGTGGAAAAG GCAGAGGTGA AGGAGAGAAC AGAGGAGCCT 
ATGGAGACAG AGGCCAAAGG TACTACTGAG GTGGAGAAAG TGGAGGAGAA GTCTGCGGTA 
GACCTGACCC CGATCGTGGT GGAAGACAAA GAAGAGAAGA AGGAGGAGGA GGAGAAAAAG 
GACGTGATGC TTCAGAATGG AGAAACTCCC AAGGATCTTA GTGATGAGAA GCAGAAGAAA 
AACAGCAAAC AGCGTTTCAT GTTCAACATT GCAGATGGTG GTTTTACTGA GTTGCACTCA 
CTGTGGCAGA ATGAAGAGCG GGCAGCCACA GTCACTAAGA AGACTTACGA GATCTGGCAC 
CGTCGCCATG ATTACTGGCT GCTGGCTGGC ATTATAAACC ACGGCTATGC CCGGTGGCAG 
GACATCCAGA ATGACCCACG GTATGCCATC CTCAATGAGC CTTTCAAGGG TGAAATGAAT 
CGTGGCAATT TTCTAGAGAT CAAGAATAAA TTTTTAGCTC GAAGATTCAA GCTCTTAGAA 
CAAGCCCTGG TGATCGAGGA GCAGCTTCGC AGGGCAGCTT ACCTGAACAT GTCCGAGGAC 
CCCTCTCACC CGTCTATGGC CCTTAACACC CGCTTCGCTG AGGTGGAGTG TTTGGCAGAA 
AGTCACCAGC ACCTGTCCAA GGAGTCGATG GCAGGGAATA AGCCGGCCAA TGCAGTCCTA 
CACAAAGGTC TCCTGAAACA GCTGGAGGAA CTGTTGAGTG ACATGAAAGC TGATGTGACG 
CGGCTCCCAG CCACCATTGC CCGCATCCCC CCAGTCGCTG TGAGGTTACA GATGTCAGAG 
CGGAACATTC TCAGCCGCCT GGCAAACAGG GCGCCAGAAC CTCCCCCACA GCAGGTAGCC 
CAGCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( NM_145979.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666091.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), mRNA.

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CloneID	OMu19517
Clone ID Related Accession (Same CDS sequence)	NM_145979.2 , NM_001346610.1
Accession Version	NM_145979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC134529.6 and AC166162.6. On Nov 17, 2006 this sequence version replaced NM_145979.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058578.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGCG AGATCATCCT GTGTGATACC 
TGTCCCCGAG CCTACCATAT GGTGTGCCTG GACCCAGACA TGGAGAAGGC CCCGGAGGGC 
AAGTGGAGCT GTCCGCACTG TGAGAAGGAG GGGATCCAGT GGGAAGCTAA GGAGGACAAT 
TCTGAGGGTG AGGAGATTCT GGAAGAAGTC GGGGGGGACC CAGAAGAGGA GGATGACCAT 
CACATGGAAT TCTGTCGCGT CTGCAAGGAC GGCGGGGAGC TCCTGTGCTG TGACACATGC 
CCTTCTTCCT ACCACATCCA CTGCCTGAAC CCCCCGCTGC CAGAGATCCC AAACGGCGAA 
TGGCTCTGTC CCCGCTGTAC TTGTCCAGCT CTTAAGGGCA AAGTGCAGAA GATCCTAATT 
TGGAAGTGGG GCCAGCCACC GTCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGACCCT 
AATACCCCGT CGCCCAAGCC CTTGGAGGGG CGGCCAGAGA GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTGTCAGAAC TGCAGTTGGA GCTGCACTGT 
CAGGTGATGT TCAGAAACTA CCAGCGGAAG AATGATATGG ACGAGCCGCC TTCTGGGGAC 
TTTGGTGGAG ATGAAGAGAA GAGCCGGAAG CGAAAGAACA AGGACCCTAA GTTTGCAGAG 
ATGGAAGAGC GCTTCTACCG CTATGGAATA AAACCGGAGT GGATGATGAT CCACCGCATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTTCACTATT TAATCAAGTG GCGAGACCTG 
CCCTATGACC AGGCGTCCTG GGAGAGCGAG GACGTGGAGA TTCAGGACTA CGACCTGTTC 
AAGCAGAGCT ACTGGAACCA CAGGGAGTTA ATGAGGGGTG AAGAAGGACG ACCCGGCAAG 
AAGCTTAAGA AGGTGAAGCT ACGGAAACTG GAAAGGCCTC CGGAGACCCC AACTGTTGAT 
CCCACCGTGA AGTATGAGCG ACAGCCGGAG TATCTGGATG CGACGGGTGG AACCCTGCAT 
CCCTACCAAA TGGAAGGCTT AAACTGGCTC CGCTTCTCCT GGGCTCAGGG AACCGATACT 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTGCAGA CAGCAGTCTT CCTCTACTCC 
CTCTATAAGG AGGGTCACTC CAAAGGCCCC TTCTTAGTGA GCGCTCCTCT GTCCACCATC 
ATCAACTGGG AGAGAGAGTT TGAGATGTGG GCTCCTGATA TGTATGTGGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGG GAAAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCGAGGATG AAGAAAGAAG CATCGGTGAA ATTCCATGTT 
CTGCTGACAT CCTATGAGTT GATCACCATT GATATGGCCA TCTTGGGTTC TATTGACTGG 
GCCTGCCTCA TTGTGGATGA AGCCCATCGG CTGAAGAACA ACCAGTCTAA GTTCTTTCGA 
GTTTTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTAA CTGGAACTCC GTTACAGAAC 
AATCTAGAGG AACTGTTTCA TCTGCTCAAC TTTCTCACCC CTGAGAGGTT CCACAACTTA 
GAAGGCTTCT TGGAGGAGTT TGCAGACATT GCCAAAGAGG ACCAGATTAA AAAACTCCAC 
GACATGCTGG GGCCTCATAT GTTGCGGCGG CTCAAAGCTG ACGTCTTCAA GAATATGCCA 
TCCAAGACAG AGCTGATTGT GCGCGTGGAG TTGAGTCCTA TGCAGAAGAA GTACTACAAG 
TATATTCTCA CGCGGAATTT CGAAGCACTT AATGCTCGAG GTGGTGGCAA CCAGGTTTCT 
CTGCTGAACG TGGTGATGGA TCTTAAGAAA TGCTGCAACC ACCCTTATCT CTTCCCTGTG 
GCAGCAATGG AAGCCCCTAA GATGCCTAAT GGTATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AGTTGTTGCT GCTGCAGAAG ATGCTTAAGA ACCTGAAAGA GGGAGGGCAT 
CGTGTGCTCA TCTTCTCCCA GATGACCAAG ATGTTGGACT TGCTAGAGGA TTTTTTAGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGTGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGATC GCTTCAATGC ACCGGGTGCT CAACAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGGATCAA TCTGGCCACT GCGGACACAG TTATTATATA TGACTCTGAC 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCCC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TCTACCGGTT TGTGACCCGT GCATCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AGATGATGCT GACACATTTA GTGGTACGGC CTGGCCTGGG CTCCAAGACA 
GGCTCAATGT CTAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAGCTATTC 
AAGGATGAAG CCACGGATGG AGGAGGAGAC AACAAGGAGG GAGAAGACAG CAGTGTTATC 
CACTATGACG ATAAGGCCAT TGAACGACTG CTGGATCGAA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCTCA GTATGTGGTA 
CGGGAAGAAG AGATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATAAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCACAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCCTCAG AGGAAGGTGA TGAAGACTTT GACGAACGGT CAGAAGCTCC CCGCAGGCCC 
AGTCGCAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTAC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCATT TCTTAATGCA 
ATTATGCGAT ATGGCATGCC ACCTCAGGAT GCTTTTACCA CTCAGTGGCT TGTGAGAGAT 
CTTCGAGGCA AGTCAGAGAA AGAATTTAAG GCTTATGTGT CACTCTTCAT GCGACATTTG 
TGTGAGCCTG GGGCAGATGG GGCTGAGACC TTTGCTGATG GCGTCCCACG AGAAGGCCTG 
TCTCGTCAAC ATGTTCTCAC TAGGATCGGT GTCATGTCTC TGATTCGAAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG ACGCTGGAGC ATGCCCGAAC TGGCTGAAGT AGAGGAAAAC 
AAAAAAATGT CTCAGCCAGG TTCACCTTCT CCAAAGACTC CTACACCTTC CACACCAGGG 
GACACACAAC CCAATACTCC TGCGCCTGTG CCACCTGCTG AGGATGGGAT AAAAATAGAG 
GAAAATAGCC TTAAAGAAGA AGAGAGCACA GAAGGAGAGA AGGAAGTTAA ATCTACAGCC 
CCTGAGGCGA CTGTGGAGTG TGCACAGCCC CCTGCCCCTG CCCCTGCCAC TGCCCCTGCC 
ACTGCCACTG CCCCTGAGGA TGACAAGGCT CCTGCTGAGC CTCCTGAGGG GGAGGAAAAA 
GTGGAAAAGG CAGAGGTGAA GGAGAGAACA GAGGAGCCTA TGGAGACAGA GGCCAAAGGT 
ACTACTGAGG TGGAGAAAGT GGAGGAGAAG TCTGCGGTAG ACCTGACCCC GATCGTGGTG 
GAAGACAAAG AAGAGAAGAA GGAGGAGGAG GAGAAAAAGG ACGTGATGCT TCAGAATGGA 
GAAACTCCCA AGGATCTTAG TGATGAGAAG CAGAAGAAAA ACAGCAAACA GCGTTTCATG 
TTCAACATTG CAGATGGTGG TTTTACTGAG TTGCACTCAC TGTGGCAGAA TGAAGAGCGG 
GCAGCCACAG TCACTAAGAA GACTTACGAG ATCTGGCACC GTCGCCATGA TTACTGGCTG 
CTGGCTGGCA TTATAAACCA CGGCTATGCC CGGTGGCAGG ACATCCAGAA TGACCCACGG 
TATGCCATCC TCAATGAGCC TTTCAAGGGT GAAATGAATC GTGGCAATTT TCTAGAGATC 
AAGAATAAAT TTTTAGCTCG AAGATTCAAG CTCTTAGAAC AAGCCCTGGT GATCGAGGAG 
CAGCTTCGCA GGGCAGCTTA CCTGAACATG TCCGAGGACC CCTCTCACCC GTCTATGGCC 
CTTAACACCC GCTTCGCTGA GGTGGAGTGT TTGGCAGAAA GTCACCAGCA CCTGTCCAAG 
GAGTCGATGG CAGGGAATAA GCCGGCCAAT GCAGTCCTAC ACAAAGTCCT GAAACAGCTG 
GAGGAACTGT TGAGTGACAT GAAAGCTGAT GTGACGCGGC TCCCAGCCAC CATTGCCCGC 
ATCCCCCCAG TCGCTGTGAG GTTACAGATG TCAGAGCGGA ACATTCTCAG CCGCCTGGCA 
AACAGGGCGC CAGAACCTCC CCCACAGCAG GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666091.1
CDS	178..5925
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	NM_145979.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145979.2

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGCG AGATCATCCT GTGTGATACC 
TGTCCCCGAG CCTACCATAT GGTGTGCCTG GACCCAGACA TGGAGAAGGC CCCGGAGGGC 
AAGTGGAGCT GTCCGCACTG TGAGAAGGAG GGGATCCAGT GGGAAGCTAA GGAGGACAAT 
TCTGAGGGTG AGGAGATTCT GGAAGAAGTC GGGGGGGACC CAGAAGAGGA GGATGACCAT 
CACATGGAAT TCTGTCGCGT CTGCAAGGAC GGCGGGGAGC TCCTGTGCTG TGACACATGC 
CCTTCTTCCT ACCACATCCA CTGCCTGAAC CCCCCGCTGC CAGAGATCCC AAACGGCGAA 
TGGCTCTGTC CCCGCTGTAC TTGTCCAGCT CTTAAGGGCA AAGTGCAGAA GATCCTAATT 
TGGAAGTGGG GCCAGCCACC GTCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGACCCT 
AATACCCCGT CGCCCAAGCC CTTGGAGGGG CGGCCAGAGA GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTGTCAGAAC TGCAGTTGGA GCTGCACTGT 
CAGGTGATGT TCAGAAACTA CCAGCGGAAG AATGATATGG ACGAGCCGCC TTCTGGGGAC 
TTTGGTGGAG ATGAAGAGAA GAGCCGGAAG CGAAAGAACA AGGACCCTAA GTTTGCAGAG 
ATGGAAGAGC GCTTCTACCG CTATGGAATA AAACCGGAGT GGATGATGAT CCACCGCATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTTCACTATT TAATCAAGTG GCGAGACCTG 
CCCTATGACC AGGCGTCCTG GGAGAGCGAG GACGTGGAGA TTCAGGACTA CGACCTGTTC 
AAGCAGAGCT ACTGGAACCA CAGGGAGTTA ATGAGGGGTG AAGAAGGACG ACCCGGCAAG 
AAGCTTAAGA AGGTGAAGCT ACGGAAACTG GAAAGGCCTC CGGAGACCCC AACTGTTGAT 
CCCACCGTGA AGTATGAGCG ACAGCCGGAG TATCTGGATG CGACGGGTGG AACCCTGCAT 
CCCTACCAAA TGGAAGGCTT AAACTGGCTC CGCTTCTCCT GGGCTCAGGG AACCGATACT 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTGCAGA CAGCAGTCTT CCTCTACTCC 
CTCTATAAGG AGGGTCACTC CAAAGGCCCC TTCTTAGTGA GCGCTCCTCT GTCCACCATC 
ATCAACTGGG AGAGAGAGTT TGAGATGTGG GCTCCTGATA TGTATGTGGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGG GAAAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCGAGGATG AAGAAAGAAG CATCGGTGAA ATTCCATGTT 
CTGCTGACAT CCTATGAGTT GATCACCATT GATATGGCCA TCTTGGGTTC TATTGACTGG 
GCCTGCCTCA TTGTGGATGA AGCCCATCGG CTGAAGAACA ACCAGTCTAA GTTCTTTCGA 
GTTTTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTAA CTGGAACTCC GTTACAGAAC 
AATCTAGAGG AACTGTTTCA TCTGCTCAAC TTTCTCACCC CTGAGAGGTT CCACAACTTA 
GAAGGCTTCT TGGAGGAGTT TGCAGACATT GCCAAAGAGG ACCAGATTAA AAAACTCCAC 
GACATGCTGG GGCCTCATAT GTTGCGGCGG CTCAAAGCTG ACGTCTTCAA GAATATGCCA 
TCCAAGACAG AGCTGATTGT GCGCGTGGAG TTGAGTCCTA TGCAGAAGAA GTACTACAAG 
TATATTCTCA CGCGGAATTT CGAAGCACTT AATGCTCGAG GTGGTGGCAA CCAGGTTTCT 
CTGCTGAACG TGGTGATGGA TCTTAAGAAA TGCTGCAACC ACCCTTATCT CTTCCCTGTG 
GCAGCAATGG AAGCCCCTAA GATGCCTAAT GGTATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AGTTGTTGCT GCTGCAGAAG ATGCTTAAGA ACCTGAAAGA GGGAGGGCAT 
CGTGTGCTCA TCTTCTCCCA GATGACCAAG ATGTTGGACT TGCTAGAGGA TTTTTTAGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGTGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGATC GCTTCAATGC ACCGGGTGCT CAACAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGGATCAA TCTGGCCACT GCGGACACAG TTATTATATA TGACTCTGAC 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCCC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TCTACCGGTT TGTGACCCGT GCATCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AGATGATGCT GACACATTTA GTGGTACGGC CTGGCCTGGG CTCCAAGACA 
GGCTCAATGT CTAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAGCTATTC 
AAGGATGAAG CCACGGATGG AGGAGGAGAC AACAAGGAGG GAGAAGACAG CAGTGTTATC 
CACTATGACG ATAAGGCCAT TGAACGACTG CTGGATCGAA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCTCA GTATGTGGTA 
CGGGAAGAAG AGATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATAAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCACAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCCTCAG AGGAAGGTGA TGAAGACTTT GACGAACGGT CAGAAGCTCC CCGCAGGCCC 
AGTCGCAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTAC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCATT TCTTAATGCA 
ATTATGCGAT ATGGCATGCC ACCTCAGGAT GCTTTTACCA CTCAGTGGCT TGTGAGAGAT 
CTTCGAGGCA AGTCAGAGAA AGAATTTAAG GCTTATGTGT CACTCTTCAT GCGACATTTG 
TGTGAGCCTG GGGCAGATGG GGCTGAGACC TTTGCTGATG GCGTCCCACG AGAAGGCCTG 
TCTCGTCAAC ATGTTCTCAC TAGGATCGGT GTCATGTCTC TGATTCGAAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG ACGCTGGAGC ATGCCCGAAC TGGCTGAAGT AGAGGAAAAC 
AAAAAAATGT CTCAGCCAGG TTCACCTTCT CCAAAGACTC CTACACCTTC CACACCAGGG 
GACACACAAC CCAATACTCC TGCGCCTGTG CCACCTGCTG AGGATGGGAT AAAAATAGAG 
GAAAATAGCC TTAAAGAAGA AGAGAGCACA GAAGGAGAGA AGGAAGTTAA ATCTACAGCC 
CCTGAGGCGA CTGTGGAGTG TGCACAGCCC CCTGCCCCTG CCCCTGCCAC TGCCCCTGCC 
ACTGCCACTG CCCCTGAGGA TGACAAGGCT CCTGCTGAGC CTCCTGAGGG GGAGGAAAAA 
GTGGAAAAGG CAGAGGTGAA GGAGAGAACA GAGGAGCCTA TGGAGACAGA GGCCAAAGGT 
ACTACTGAGG TGGAGAAAGT GGAGGAGAAG TCTGCGGTAG ACCTGACCCC GATCGTGGTG 
GAAGACAAAG AAGAGAAGAA GGAGGAGGAG GAGAAAAAGG ACGTGATGCT TCAGAATGGA 
GAAACTCCCA AGGATCTTAG TGATGAGAAG CAGAAGAAAA ACAGCAAACA GCGTTTCATG 
TTCAACATTG CAGATGGTGG TTTTACTGAG TTGCACTCAC TGTGGCAGAA TGAAGAGCGG 
GCAGCCACAG TCACTAAGAA GACTTACGAG ATCTGGCACC GTCGCCATGA TTACTGGCTG 
CTGGCTGGCA TTATAAACCA CGGCTATGCC CGGTGGCAGG ACATCCAGAA TGACCCACGG 
TATGCCATCC TCAATGAGCC TTTCAAGGGT GAAATGAATC GTGGCAATTT TCTAGAGATC 
AAGAATAAAT TTTTAGCTCG AAGATTCAAG CTCTTAGAAC AAGCCCTGGT GATCGAGGAG 
CAGCTTCGCA GGGCAGCTTA CCTGAACATG TCCGAGGACC CCTCTCACCC GTCTATGGCC 
CTTAACACCC GCTTCGCTGA GGTGGAGTGT TTGGCAGAAA GTCACCAGCA CCTGTCCAAG 
GAGTCGATGG CAGGGAATAA GCCGGCCAAT GCAGTCCTAC ACAAAGTCCT GAAACAGCTG 
GAGGAACTGT TGAGTGACAT GAAAGCTGAT GTGACGCGGC TCCCAGCCAC CATTGCCCGC 
ATCCCCCCAG TCGCTGTGAG GTTACAGATG TCAGAGCGGA ACATTCTCAG CCGCCTGGCA 
AACAGGGCGC CAGAACCTCC CCCACAGCAG GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( NM_001346610.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001333539.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), mRNA.

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CloneID	OMu19517
Clone ID Related Accession (Same CDS sequence)	NM_145979.2 , NM_001346610.1
Accession Version	NM_001346610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147695.1, AK145532.1, AC134529.6, AK034549.1, CN527871.1, DV661376.1, CX565499.1 and AI844224.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058578.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGCG AGATCATCCT GTGTGATACC 
TGTCCCCGAG CCTACCATAT GGTGTGCCTG GACCCAGACA TGGAGAAGGC CCCGGAGGGC 
AAGTGGAGCT GTCCGCACTG TGAGAAGGAG GGGATCCAGT GGGAAGCTAA GGAGGACAAT 
TCTGAGGGTG AGGAGATTCT GGAAGAAGTC GGGGGGGACC CAGAAGAGGA GGATGACCAT 
CACATGGAAT TCTGTCGCGT CTGCAAGGAC GGCGGGGAGC TCCTGTGCTG TGACACATGC 
CCTTCTTCCT ACCACATCCA CTGCCTGAAC CCCCCGCTGC CAGAGATCCC AAACGGCGAA 
TGGCTCTGTC CCCGCTGTAC TTGTCCAGCT CTTAAGGGCA AAGTGCAGAA GATCCTAATT 
TGGAAGTGGG GCCAGCCACC GTCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGACCCT 
AATACCCCGT CGCCCAAGCC CTTGGAGGGG CGGCCAGAGA GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTGTCAGAAC TGCAGTTGGA GCTGCACTGT 
CAGGTGATGT TCAGAAACTA CCAGCGGAAG AATGATATGG ACGAGCCGCC TTCTGGGGAC 
TTTGGTGGAG ATGAAGAGAA GAGCCGGAAG CGAAAGAACA AGGACCCTAA GTTTGCAGAG 
ATGGAAGAGC GCTTCTACCG CTATGGAATA AAACCGGAGT GGATGATGAT CCACCGCATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTTCACTATT TAATCAAGTG GCGAGACCTG 
CCCTATGACC AGGCGTCCTG GGAGAGCGAG GACGTGGAGA TTCAGGACTA CGACCTGTTC 
AAGCAGAGCT ACTGGAACCA CAGGGAGTTA ATGAGGGGTG AAGAAGGACG ACCCGGCAAG 
AAGCTTAAGA AGGTGAAGCT ACGGAAACTG GAAAGGCCTC CGGAGACCCC AACTGTTGAT 
CCCACCGTGA AGTATGAGCG ACAGCCGGAG TATCTGGATG CGACGGGTGG AACCCTGCAT 
CCCTACCAAA TGGAAGGCTT AAACTGGCTC CGCTTCTCCT GGGCTCAGGG AACCGATACT 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTGCAGA CAGCAGTCTT CCTCTACTCC 
CTCTATAAGG AGGGTCACTC CAAAGGCCCC TTCTTAGTGA GCGCTCCTCT GTCCACCATC 
ATCAACTGGG AGAGAGAGTT TGAGATGTGG GCTCCTGATA TGTATGTGGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGG GAAAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCGAGGATG AAGAAAGAAG CATCGGTGAA ATTCCATGTT 
CTGCTGACAT CCTATGAGTT GATCACCATT GATATGGCCA TCTTGGGTTC TATTGACTGG 
GCCTGCCTCA TTGTGGATGA AGCCCATCGG CTGAAGAACA ACCAGTCTAA GTTCTTTCGA 
GTTTTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTAA CTGGAACTCC GTTACAGAAC 
AATCTAGAGG AACTGTTTCA TCTGCTCAAC TTTCTCACCC CTGAGAGGTT CCACAACTTA 
GAAGGCTTCT TGGAGGAGTT TGCAGACATT GCCAAAGAGG ACCAGATTAA AAAACTCCAC 
GACATGCTGG GGCCTCATAT GTTGCGGCGG CTCAAAGCTG ACGTCTTCAA GAATATGCCA 
TCCAAGACAG AGCTGATTGT GCGCGTGGAG TTGAGTCCTA TGCAGAAGAA GTACTACAAG 
TATATTCTCA CGCGGAATTT CGAAGCACTT AATGCTCGAG GTGGTGGCAA CCAGGTTTCT 
CTGCTGAACG TGGTGATGGA TCTTAAGAAA TGCTGCAACC ACCCTTATCT CTTCCCTGTG 
GCAGCAATGG AAGCCCCTAA GATGCCTAAT GGTATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AGTTGTTGCT GCTGCAGAAG ATGCTTAAGA ACCTGAAAGA GGGAGGGCAT 
CGTGTGCTCA TCTTCTCCCA GATGACCAAG ATGTTGGACT TGCTAGAGGA TTTTTTAGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGTGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGATC GCTTCAATGC ACCGGGTGCT CAACAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGGATCAA TCTGGCCACT GCGGACACAG TTATTATATA TGACTCTGAC 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCCC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TCTACCGGTT TGTGACCCGT GCATCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AGATGATGCT GACACATTTA GTGGTACGGC CTGGCCTGGG CTCCAAGACA 
GGCTCAATGT CTAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAGCTATTC 
AAGGATGAAG CCACGGATGG AGGAGGAGAC AACAAGGAGG GAGAAGACAG CAGTGTTATC 
CACTATGACG ATAAGGCCAT TGAACGACTG CTGGATCGAA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCTCA GTATGTGGTA 
CGGGAAGAAG AGATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATAAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCACAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCCTCAG AGGAAGGTGA TGAAGACTTT GACGAACGGT CAGAAGCTCC CCGCAGGCCC 
AGTCGCAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTAC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCATT TCTTAATGCA 
ATTATGCGAT ATGGCATGCC ACCTCAGGAT GCTTTTACCA CTCAGTGGCT TGTGAGAGAT 
CTTCGAGGCA AGTCAGAGAA AGAATTTAAG GCTTATGTGT CACTCTTCAT GCGACATTTG 
TGTGAGCCTG GGGCAGATGG GGCTGAGACC TTTGCTGATG GCGTCCCACG AGAAGGCCTG 
TCTCGTCAAC ATGTTCTCAC TAGGATCGGT GTCATGTCTC TGATTCGAAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG ACGCTGGAGC ATGCCCGAAC TGGCTGAAGT AGAGGAAAAC 
AAAAAAATGT CTCAGCCAGG TTCACCTTCT CCAAAGACTC CTACACCTTC CACACCAGGG 
GACACACAAC CCAATACTCC TGCGCCTGTG CCACCTGCTG AGGATGGGAT AAAAATAGAG 
GAAAATAGCC TTAAAGAAGA AGAGAGCACA GAAGGAGAGA AGGAAGTTAA ATCTACAGCC 
CCTGAGGCGA CTGTGGAGTG TGCACAGCCC CCTGCCCCTG CCCCTGCCAC TGCCCCTGCC 
ACTGCCACTG CCCCTGAGGA TGACAAGGCT CCTGCTGAGC CTCCTGAGGG GGAGGAAAAA 
GTGGAAAAGG CAGAGGTGAA GGAGAGAACA GAGGAGCCTA TGGAGACAGA GGCCAAAGGT 
ACTACTGAGG TGGAGAAAGT GGAGGAGAAG TCTGCGGTAG ACCTGACCCC GATCGTGGTG 
GAAGACAAAG AAGAGAAGAA GGAGGAGGAG GAGAAAAAGG ACGTGATGCT TCAGAATGGA 
GAAACTCCCA AGGATCTTAG TGATGAGAAG CAGAAGAAAA ACAGCAAACA GCGTTTCATG 
TTCAACATTG CAGATGGTGG TTTTACTGAG TTGCACTCAC TGTGGCAGAA TGAAGAGCGG 
GCAGCCACAG TCACTAAGAA GACTTACGAG ATCTGGCACC GTCGCCATGA TTACTGGCTG 
CTGGCTGGCA TTATAAACCA CGGCTATGCC CGGTGGCAGG ACATCCAGAA TGACCCACGG 
TATGCCATCC TCAATGAGCC TTTCAAGGGT GAAATGAATC GTGGCAATTT TCTAGAGATC 
AAGAATAAAT TTTTAGCTCG AAGATTCAAG CTCTTAGAAC AAGCCCTGGT GATCGAGGAG 
CAGCTTCGCA GGGCAGCTTA CCTGAACATG TCCGAGGACC CCTCTCACCC GTCTATGGCC 
CTTAACACCC GCTTCGCTGA GGTGGAGTGT TTGGCAGAAA GTCACCAGCA CCTGTCCAAG 
GAGTCGATGG CAGGGAATAA GCCGGCCAAT GCAGTCCTAC ACAAAGTCCT GAAACAGCTG 
GAGGAACTGT TGAGTGACAT GAAAGCTGAT GTGACGCGGC TCCCAGCCAC CATTGCCCGC 
ATCCCCCCAG TCGCTGTGAG GTTACAGATG TCAGAGCGGA ACATTCTCAG CCGCCTGGCA 
AACAGGGCGC CAGAACCTCC CCCACAGCAG GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333539.1
CDS	360..6107
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	NM_001346610.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346610.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGCG AGATCATCCT GTGTGATACC 
TGTCCCCGAG CCTACCATAT GGTGTGCCTG GACCCAGACA TGGAGAAGGC CCCGGAGGGC 
AAGTGGAGCT GTCCGCACTG TGAGAAGGAG GGGATCCAGT GGGAAGCTAA GGAGGACAAT 
TCTGAGGGTG AGGAGATTCT GGAAGAAGTC GGGGGGGACC CAGAAGAGGA GGATGACCAT 
CACATGGAAT TCTGTCGCGT CTGCAAGGAC GGCGGGGAGC TCCTGTGCTG TGACACATGC 
CCTTCTTCCT ACCACATCCA CTGCCTGAAC CCCCCGCTGC CAGAGATCCC AAACGGCGAA 
TGGCTCTGTC CCCGCTGTAC TTGTCCAGCT CTTAAGGGCA AAGTGCAGAA GATCCTAATT 
TGGAAGTGGG GCCAGCCACC GTCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGACCCT 
AATACCCCGT CGCCCAAGCC CTTGGAGGGG CGGCCAGAGA GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTGTCAGAAC TGCAGTTGGA GCTGCACTGT 
CAGGTGATGT TCAGAAACTA CCAGCGGAAG AATGATATGG ACGAGCCGCC TTCTGGGGAC 
TTTGGTGGAG ATGAAGAGAA GAGCCGGAAG CGAAAGAACA AGGACCCTAA GTTTGCAGAG 
ATGGAAGAGC GCTTCTACCG CTATGGAATA AAACCGGAGT GGATGATGAT CCACCGCATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTTCACTATT TAATCAAGTG GCGAGACCTG 
CCCTATGACC AGGCGTCCTG GGAGAGCGAG GACGTGGAGA TTCAGGACTA CGACCTGTTC 
AAGCAGAGCT ACTGGAACCA CAGGGAGTTA ATGAGGGGTG AAGAAGGACG ACCCGGCAAG 
AAGCTTAAGA AGGTGAAGCT ACGGAAACTG GAAAGGCCTC CGGAGACCCC AACTGTTGAT 
CCCACCGTGA AGTATGAGCG ACAGCCGGAG TATCTGGATG CGACGGGTGG AACCCTGCAT 
CCCTACCAAA TGGAAGGCTT AAACTGGCTC CGCTTCTCCT GGGCTCAGGG AACCGATACT 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTGCAGA CAGCAGTCTT CCTCTACTCC 
CTCTATAAGG AGGGTCACTC CAAAGGCCCC TTCTTAGTGA GCGCTCCTCT GTCCACCATC 
ATCAACTGGG AGAGAGAGTT TGAGATGTGG GCTCCTGATA TGTATGTGGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGG GAAAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCGAGGATG AAGAAAGAAG CATCGGTGAA ATTCCATGTT 
CTGCTGACAT CCTATGAGTT GATCACCATT GATATGGCCA TCTTGGGTTC TATTGACTGG 
GCCTGCCTCA TTGTGGATGA AGCCCATCGG CTGAAGAACA ACCAGTCTAA GTTCTTTCGA 
GTTTTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTAA CTGGAACTCC GTTACAGAAC 
AATCTAGAGG AACTGTTTCA TCTGCTCAAC TTTCTCACCC CTGAGAGGTT CCACAACTTA 
GAAGGCTTCT TGGAGGAGTT TGCAGACATT GCCAAAGAGG ACCAGATTAA AAAACTCCAC 
GACATGCTGG GGCCTCATAT GTTGCGGCGG CTCAAAGCTG ACGTCTTCAA GAATATGCCA 
TCCAAGACAG AGCTGATTGT GCGCGTGGAG TTGAGTCCTA TGCAGAAGAA GTACTACAAG 
TATATTCTCA CGCGGAATTT CGAAGCACTT AATGCTCGAG GTGGTGGCAA CCAGGTTTCT 
CTGCTGAACG TGGTGATGGA TCTTAAGAAA TGCTGCAACC ACCCTTATCT CTTCCCTGTG 
GCAGCAATGG AAGCCCCTAA GATGCCTAAT GGTATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AGTTGTTGCT GCTGCAGAAG ATGCTTAAGA ACCTGAAAGA GGGAGGGCAT 
CGTGTGCTCA TCTTCTCCCA GATGACCAAG ATGTTGGACT TGCTAGAGGA TTTTTTAGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGTGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGATC GCTTCAATGC ACCGGGTGCT CAACAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGGATCAA TCTGGCCACT GCGGACACAG TTATTATATA TGACTCTGAC 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCCC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TCTACCGGTT TGTGACCCGT GCATCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AGATGATGCT GACACATTTA GTGGTACGGC CTGGCCTGGG CTCCAAGACA 
GGCTCAATGT CTAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAGCTATTC 
AAGGATGAAG CCACGGATGG AGGAGGAGAC AACAAGGAGG GAGAAGACAG CAGTGTTATC 
CACTATGACG ATAAGGCCAT TGAACGACTG CTGGATCGAA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCTCA GTATGTGGTA 
CGGGAAGAAG AGATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATAAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCACAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCCTCAG AGGAAGGTGA TGAAGACTTT GACGAACGGT CAGAAGCTCC CCGCAGGCCC 
AGTCGCAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTAC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCATT TCTTAATGCA 
ATTATGCGAT ATGGCATGCC ACCTCAGGAT GCTTTTACCA CTCAGTGGCT TGTGAGAGAT 
CTTCGAGGCA AGTCAGAGAA AGAATTTAAG GCTTATGTGT CACTCTTCAT GCGACATTTG 
TGTGAGCCTG GGGCAGATGG GGCTGAGACC TTTGCTGATG GCGTCCCACG AGAAGGCCTG 
TCTCGTCAAC ATGTTCTCAC TAGGATCGGT GTCATGTCTC TGATTCGAAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG ACGCTGGAGC ATGCCCGAAC TGGCTGAAGT AGAGGAAAAC 
AAAAAAATGT CTCAGCCAGG TTCACCTTCT CCAAAGACTC CTACACCTTC CACACCAGGG 
GACACACAAC CCAATACTCC TGCGCCTGTG CCACCTGCTG AGGATGGGAT AAAAATAGAG 
GAAAATAGCC TTAAAGAAGA AGAGAGCACA GAAGGAGAGA AGGAAGTTAA ATCTACAGCC 
CCTGAGGCGA CTGTGGAGTG TGCACAGCCC CCTGCCCCTG CCCCTGCCAC TGCCCCTGCC 
ACTGCCACTG CCCCTGAGGA TGACAAGGCT CCTGCTGAGC CTCCTGAGGG GGAGGAAAAA 
GTGGAAAAGG CAGAGGTGAA GGAGAGAACA GAGGAGCCTA TGGAGACAGA GGCCAAAGGT 
ACTACTGAGG TGGAGAAAGT GGAGGAGAAG TCTGCGGTAG ACCTGACCCC GATCGTGGTG 
GAAGACAAAG AAGAGAAGAA GGAGGAGGAG GAGAAAAAGG ACGTGATGCT TCAGAATGGA 
GAAACTCCCA AGGATCTTAG TGATGAGAAG CAGAAGAAAA ACAGCAAACA GCGTTTCATG 
TTCAACATTG CAGATGGTGG TTTTACTGAG TTGCACTCAC TGTGGCAGAA TGAAGAGCGG 
GCAGCCACAG TCACTAAGAA GACTTACGAG ATCTGGCACC GTCGCCATGA TTACTGGCTG 
CTGGCTGGCA TTATAAACCA CGGCTATGCC CGGTGGCAGG ACATCCAGAA TGACCCACGG 
TATGCCATCC TCAATGAGCC TTTCAAGGGT GAAATGAATC GTGGCAATTT TCTAGAGATC 
AAGAATAAAT TTTTAGCTCG AAGATTCAAG CTCTTAGAAC AAGCCCTGGT GATCGAGGAG 
CAGCTTCGCA GGGCAGCTTA CCTGAACATG TCCGAGGACC CCTCTCACCC GTCTATGGCC 
CTTAACACCC GCTTCGCTGA GGTGGAGTGT TTGGCAGAAA GTCACCAGCA CCTGTCCAAG 
GAGTCGATGG CAGGGAATAA GCCGGCCAAT GCAGTCCTAC ACAAAGTCCT GAAACAGCTG 
GAGGAACTGT TGAGTGACAT GAAAGCTGAT GTGACGCGGC TCCCAGCCAC CATTGCCCGC 
ATCCCCCCAG TCGCTGTGAG GTTACAGATG TCAGAGCGGA ACATTCTCAG CCGCCTGGCA 
AACAGGGCGC CAGAACCTCC CCCACAGCAG GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_006505280.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505343.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu36718
Clone ID Related Accession (Same CDS sequence)	XM_006505280.1
Accession Version	XM_006505280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA CGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ACTCAGTGGC CTCAGAGGAA 
GGTGATGAAG ACTTTGACGA ACGGTCAGAA GCTCCCCGCA GGCCCAGTCG CAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTACCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTACTTGGTT TTAATGCTCG TCAGCGAAAA GCATTTCTTA ATGCAATTAT GCGATATGGC 
ATGCCACCTC AGGATGCTTT TACCACTCAG TGGCTTGTGA GAGATCTTCG AGGCAAGTCA 
GAGAAAGAAT TTAAGGCTTA TGTGTCACTC TTCATGCGAC ATTTGTGTGA GCCTGGGGCA 
GATGGGGCTG AGACCTTTGC TGATGGCGTC CCACGAGAAG GCCTGTCTCG TCAACATGTT 
CTCACTAGGA TCGGTGTCAT GTCTCTGATT CGAAAGAAGG TTCAGGAGTT TGAACATGTT 
AATGGACGCT GGAGCATGCC CGAACTGGCT GAAGTAGAGG AAAACAAAAA AATGTCTCAG 
CCAGGTTCAC CTTCTCCAAA GACTCCTACA CCTTCCACAC CAGGGGACAC ACAACCCAAT 
ACTCCTGCGC CTGTGCCACC TGCTGAGGAT GGGATAAAAA TAGAGGAAAA TAGCCTTAAA 
GAAGAAGAGA GCACAGAAGG AGAGAAGGAA GTTAAATCTA CAGCCCCTGA GGCGACTGTG 
GAGTGTGCAC AGCCCCCTGC CCCTGCCCCT GCCACTGCCC CTGCCACTGC CACTGCCCCT 
GAGGATGACA AGGCTCCTGC TGAGCCTCCT GAGGGGGAGG AAAAAGTGGA AAAGGCAGAG 
GTGAAGGAGA GAACAGAGGA GCCTATGGAG ACAGAGGCCA AAGGTACTAC TGAGGTGGAG 
AAAGTGGAGG AGAAGTCTGC GGTAGACCTG ACCCCGATCG TGGTGGAAGA CAAAGAAGAG 
AAGAAGGAGG AGGAGGAGAA AAAGGACGTG ATGCTTCAGA ATGGAGAAAC TCCCAAGGAT 
CTTAGTGATG AGAAGCAGAA GAAAAACAGC AAACAGCGTT TCATGTTCAA CATTGCAGAT 
GGTGGTTTTA CTGAGTTGCA CTCACTGTGG CAGAATGAAG AGCGGGCAGC CACAGTCACT 
AAGAAGACTT ACGAGATCTG GCACCGTCGC CATGATTACT GGCTGCTGGC TGGCATTATA 
AACCACGGCT ATGCCCGGTG GCAGGACATC CAGAATGACC CACGGTATGC CATCCTCAAT 
GAGCCTTTCA AGGGTGAAAT GAATCGTGGC AATTTTCTAG AGATCAAGAA TAAATTTTTA 
GCTCGAAGAT TCAAGCTCTT AGAACAAGCC CTGGTGATCG AGGAGCAGCT TCGCAGGGCA 
GCTTACCTGA ACATGTCCGA GGACCCCTCT CACCCGTCTA TGGCCCTTAA CACCCGCTTC 
GCTGAGGTGG AGTGTTTGGC AGAAAGTCAC CAGCACCTGT CCAAGGAGTC GATGGCAGGG 
AATAAGCCGG CCAATGCAGT CCTACACAAA GGTCTCCTGA AACAGCTGGA GGAACTGTTG 
AGTGACATGA AAGCTGATGT GACGCGGCTC CCAGCCACCA TTGCCCGCAT CCCCCCAGTC 
GCTGTGAGGT TACAGATGTC AGAGCGGAAC ATTCTCAGCC GCCTGGCAAA CAGGGCGCCA 
GAACCTCCCC CACAGCAGGT AGCCCAGCAG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505343.1
CDS	355..6210
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPTGRSTRAVGPAHLPSLPPDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGLLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006505280.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505280.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA CGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ACTCAGTGGC CTCAGAGGAA 
GGTGATGAAG ACTTTGACGA ACGGTCAGAA GCTCCCCGCA GGCCCAGTCG CAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTACCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTACTTGGTT TTAATGCTCG TCAGCGAAAA GCATTTCTTA ATGCAATTAT GCGATATGGC 
ATGCCACCTC AGGATGCTTT TACCACTCAG TGGCTTGTGA GAGATCTTCG AGGCAAGTCA 
GAGAAAGAAT TTAAGGCTTA TGTGTCACTC TTCATGCGAC ATTTGTGTGA GCCTGGGGCA 
GATGGGGCTG AGACCTTTGC TGATGGCGTC CCACGAGAAG GCCTGTCTCG TCAACATGTT 
CTCACTAGGA TCGGTGTCAT GTCTCTGATT CGAAAGAAGG TTCAGGAGTT TGAACATGTT 
AATGGACGCT GGAGCATGCC CGAACTGGCT GAAGTAGAGG AAAACAAAAA AATGTCTCAG 
CCAGGTTCAC CTTCTCCAAA GACTCCTACA CCTTCCACAC CAGGGGACAC ACAACCCAAT 
ACTCCTGCGC CTGTGCCACC TGCTGAGGAT GGGATAAAAA TAGAGGAAAA TAGCCTTAAA 
GAAGAAGAGA GCACAGAAGG AGAGAAGGAA GTTAAATCTA CAGCCCCTGA GGCGACTGTG 
GAGTGTGCAC AGCCCCCTGC CCCTGCCCCT GCCACTGCCC CTGCCACTGC CACTGCCCCT 
GAGGATGACA AGGCTCCTGC TGAGCCTCCT GAGGGGGAGG AAAAAGTGGA AAAGGCAGAG 
GTGAAGGAGA GAACAGAGGA GCCTATGGAG ACAGAGGCCA AAGGTACTAC TGAGGTGGAG 
AAAGTGGAGG AGAAGTCTGC GGTAGACCTG ACCCCGATCG TGGTGGAAGA CAAAGAAGAG 
AAGAAGGAGG AGGAGGAGAA AAAGGACGTG ATGCTTCAGA ATGGAGAAAC TCCCAAGGAT 
CTTAGTGATG AGAAGCAGAA GAAAAACAGC AAACAGCGTT TCATGTTCAA CATTGCAGAT 
GGTGGTTTTA CTGAGTTGCA CTCACTGTGG CAGAATGAAG AGCGGGCAGC CACAGTCACT 
AAGAAGACTT ACGAGATCTG GCACCGTCGC CATGATTACT GGCTGCTGGC TGGCATTATA 
AACCACGGCT ATGCCCGGTG GCAGGACATC CAGAATGACC CACGGTATGC CATCCTCAAT 
GAGCCTTTCA AGGGTGAAAT GAATCGTGGC AATTTTCTAG AGATCAAGAA TAAATTTTTA 
GCTCGAAGAT TCAAGCTCTT AGAACAAGCC CTGGTGATCG AGGAGCAGCT TCGCAGGGCA 
GCTTACCTGA ACATGTCCGA GGACCCCTCT CACCCGTCTA TGGCCCTTAA CACCCGCTTC 
GCTGAGGTGG AGTGTTTGGC AGAAAGTCAC CAGCACCTGT CCAAGGAGTC GATGGCAGGG 
AATAAGCCGG CCAATGCAGT CCTACACAAA GGTCTCCTGA AACAGCTGGA GGAACTGTTG 
AGTGACATGA AAGCTGATGT GACGCGGCTC CCAGCCACCA TTGCCCGCAT CCCCCCAGTC 
GCTGTGAGGT TACAGATGTC AGAGCGGAAC ATTCTCAGCC GCCTGGCAAA CAGGGCGCCA 
GAACCTCCCC CACAGCAGGT AGCCCAGCAG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_006505281.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505344.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X1, mRNA.

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CloneID	OMu36719
Clone ID Related Accession (Same CDS sequence)	XM_006505281.1
Accession Version	XM_006505281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA CGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ACTCAGTGGC CTCAGAGGAA 
GGTGATGAAG ACTTTGACGA ACGGTCAGAA GCTCCCCGCA GGCCCAGTCG CAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTACCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTACTTGGTT TTAATGCTCG TCAGCGAAAA GCATTTCTTA ATGCAATTAT GCGATATGGC 
ATGCCACCTC AGGATGCTTT TACCACTCAG TGGCTTGTGA GAGATCTTCG AGGCAAGTCA 
GAGAAAGAAT TTAAGGCTTA TGTGTCACTC TTCATGCGAC ATTTGTGTGA GCCTGGGGCA 
GATGGGGCTG AGACCTTTGC TGATGGCGTC CCACGAGAAG GCCTGTCTCG TCAACATGTT 
CTCACTAGGA TCGGTGTCAT GTCTCTGATT CGAAAGAAGG TTCAGGAGTT TGAACATGTT 
AATGGACGCT GGAGCATGCC CGAACTGGCT GAAGTAGAGG AAAACAAAAA AATGTCTCAG 
CCAGGTTCAC CTTCTCCAAA GACTCCTACA CCTTCCACAC CAGGGGACAC ACAACCCAAT 
ACTCCTGCGC CTGTGCCACC TGCTGAGGAT GGGATAAAAA TAGAGGAAAA TAGCCTTAAA 
GAAGAAGAGA GCACAGAAGG AGAGAAGGAA GTTAAATCTA CAGCCCCTGA GGCGACTGTG 
GAGTGTGCAC AGCCCCCTGC CCCTGCCCCT GCCACTGCCC CTGCCACTGC CACTGCCCCT 
GAGGATGACA AGGCTCCTGC TGAGCCTCCT GAGGGGGAGG AAAAAGTGGA AAAGGCAGAG 
GTGAAGGAGA GAACAGAGGA GCCTATGGAG ACAGAGGCCA AAGGTACTAC TGAGGTGGAG 
AAAGTGGAGG AGAAGTCTGC GGTAGACCTG ACCCCGATCG TGGTGGAAGA CAAAGAAGAG 
AAGAAGGAGG AGGAGGAGAA AAAGGACGTG ATGCTTCAGA ATGGAGAAAC TCCCAAGGAT 
CTTAGTGATG AGAAGCAGAA GAAAAACAGC AAACAGCGTT TCATGTTCAA CATTGCAGAT 
GGTGGTTTTA CTGAGTTGCA CTCACTGTGG CAGAATGAAG AGCGGGCAGC CACAGTCACT 
AAGAAGACTT ACGAGATCTG GCACCGTCGC CATGATTACT GGCTGCTGGC TGGCATTATA 
AACCACGGCT ATGCCCGGTG GCAGGACATC CAGAATGACC CACGGTATGC CATCCTCAAT 
GAGCCTTTCA AGGGTGAAAT GAATCGTGGC AATTTTCTAG AGATCAAGAA TAAATTTTTA 
GCTCGAAGAT TCAAGCTCTT AGAACAAGCC CTGGTGATCG AGGAGCAGCT TCGCAGGGCA 
GCTTACCTGA ACATGTCCGA GGACCCCTCT CACCCGTCTA TGGCCCTTAA CACCCGCTTC 
GCTGAGGTGG AGTGTTTGGC AGAAAGTCAC CAGCACCTGT CCAAGGAGTC GATGGCAGGG 
AATAAGCCGG CCAATGCAGT CCTACACAAA GTCCTGAAAC AGCTGGAGGA ACTGTTGAGT 
GACATGAAAG CTGATGTGAC GCGGCTCCCA GCCACCATTG CCCGCATCCC CCCAGTCGCT 
GTGAGGTTAC AGATGTCAGA GCGGAACATT CTCAGCCGCC TGGCAAACAG GGCGCCAGAA 
CCTCCCCCAC AGCAGGTAGC CCAGCAGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505344.1
CDS	355..6207
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPTGRSTRAVGPAHLPSLPPDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006505281.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505281.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA CGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ACTCAGTGGC CTCAGAGGAA 
GGTGATGAAG ACTTTGACGA ACGGTCAGAA GCTCCCCGCA GGCCCAGTCG CAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTACCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTACTTGGTT TTAATGCTCG TCAGCGAAAA GCATTTCTTA ATGCAATTAT GCGATATGGC 
ATGCCACCTC AGGATGCTTT TACCACTCAG TGGCTTGTGA GAGATCTTCG AGGCAAGTCA 
GAGAAAGAAT TTAAGGCTTA TGTGTCACTC TTCATGCGAC ATTTGTGTGA GCCTGGGGCA 
GATGGGGCTG AGACCTTTGC TGATGGCGTC CCACGAGAAG GCCTGTCTCG TCAACATGTT 
CTCACTAGGA TCGGTGTCAT GTCTCTGATT CGAAAGAAGG TTCAGGAGTT TGAACATGTT 
AATGGACGCT GGAGCATGCC CGAACTGGCT GAAGTAGAGG AAAACAAAAA AATGTCTCAG 
CCAGGTTCAC CTTCTCCAAA GACTCCTACA CCTTCCACAC CAGGGGACAC ACAACCCAAT 
ACTCCTGCGC CTGTGCCACC TGCTGAGGAT GGGATAAAAA TAGAGGAAAA TAGCCTTAAA 
GAAGAAGAGA GCACAGAAGG AGAGAAGGAA GTTAAATCTA CAGCCCCTGA GGCGACTGTG 
GAGTGTGCAC AGCCCCCTGC CCCTGCCCCT GCCACTGCCC CTGCCACTGC CACTGCCCCT 
GAGGATGACA AGGCTCCTGC TGAGCCTCCT GAGGGGGAGG AAAAAGTGGA AAAGGCAGAG 
GTGAAGGAGA GAACAGAGGA GCCTATGGAG ACAGAGGCCA AAGGTACTAC TGAGGTGGAG 
AAAGTGGAGG AGAAGTCTGC GGTAGACCTG ACCCCGATCG TGGTGGAAGA CAAAGAAGAG 
AAGAAGGAGG AGGAGGAGAA AAAGGACGTG ATGCTTCAGA ATGGAGAAAC TCCCAAGGAT 
CTTAGTGATG AGAAGCAGAA GAAAAACAGC AAACAGCGTT TCATGTTCAA CATTGCAGAT 
GGTGGTTTTA CTGAGTTGCA CTCACTGTGG CAGAATGAAG AGCGGGCAGC CACAGTCACT 
AAGAAGACTT ACGAGATCTG GCACCGTCGC CATGATTACT GGCTGCTGGC TGGCATTATA 
AACCACGGCT ATGCCCGGTG GCAGGACATC CAGAATGACC CACGGTATGC CATCCTCAAT 
GAGCCTTTCA AGGGTGAAAT GAATCGTGGC AATTTTCTAG AGATCAAGAA TAAATTTTTA 
GCTCGAAGAT TCAAGCTCTT AGAACAAGCC CTGGTGATCG AGGAGCAGCT TCGCAGGGCA 
GCTTACCTGA ACATGTCCGA GGACCCCTCT CACCCGTCTA TGGCCCTTAA CACCCGCTTC 
GCTGAGGTGG AGTGTTTGGC AGAAAGTCAC CAGCACCTGT CCAAGGAGTC GATGGCAGGG 
AATAAGCCGG CCAATGCAGT CCTACACAAA GTCCTGAAAC AGCTGGAGGA ACTGTTGAGT 
GACATGAAAG CTGATGTGAC GCGGCTCCCA GCCACCATTG CCCGCATCCC CCCAGTCGCT 
GTGAGGTTAC AGATGTCAGA GCGGAACATT CTCAGCCGCC TGGCAAACAG GGCGCCAGAA 
CCTCCCCCAC AGCAGGTAGC CCAGCAGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_006505282.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505345.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X4, mRNA.

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CloneID	OMu36720
Clone ID Related Accession (Same CDS sequence)	XM_006505282.1
Accession Version	XM_006505282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGCG AGATCATCCT GTGTGATACC 
TGTCCCCGAG CCTACCATAT GGTGTGCCTG GACCCAGACA TGGAGAAGGC CCCGGAGGGC 
AAGTGGAGCT GTCCGCACTG TGAGAAGGAG GGGATCCAGT GGGAAGCTAA GGAGGACAAT 
TCTGAGGGTG AGGAGATTCT GGAAGAAGTC GGGGGGGACC CAGAAGAGGA GGATGACCAT 
CACATGGAAT TCTGTCGCGT CTGCAAGGAC GGCGGGGAGC TCCTGTGCTG TGACACATGC 
CCTTCTTCCT ACCACATCCA CTGCCTGAAC CCCCCGCTGC CAGAGATCCC AAACGGCGAA 
TGGCTCTGTC CCCGCTGTAC TTGTCCAGCT CTTAAGGGCA AAGTGCAGAA GATCCTAATT 
TGGAAGTGGG GCCAGCCACC GTCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGACCCT 
AATACCCCGT CGCCCAAGCC CTTGGAGGGG CGGCCAGAGA GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTGTCAGAAC TGCAGTTGGA GCTGCACTGT 
CAGGTGATGT TCAGAAACTA CCAGCGGAAG AATGATATGG ACGAGCCGCC TTCTGGGGAC 
TTTGGTGGAG ATGAAGAGAA GAGCCGGAAG CGAAAGAACA AGGACCCTAA GTTTGCAGAG 
ATGGAAGAGC GCTTCTACCG CTATGGAATA AAACCGGAGT GGATGATGAT CCACCGCATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTTCACTATT TAATCAAGTG GCGAGACCTG 
CCCTATGACC AGGCGTCCTG GGAGAGCGAG GACGTGGAGA TTCAGGACTA CGACCTGTTC 
AAGCAGAGCT ACTGGAACCA CAGGGAGTTA ATGAGGGGTG AAGAAGGACG ACCCGGCAAG 
AAGCTTAAGA AGGTGAAGCT ACGGAAACTG GAAAGGCCTC CGGAGACCCC AACTGTTGAT 
CCCACCGTGA AGTATGAGCG ACAGCCGGAG TATCTGGATG CGACGGGTGG AACCCTGCAT 
CCCTACCAAA TGGAAGGCTT AAACTGGCTC CGCTTCTCCT GGGCTCAGGG AACCGATACT 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTGCAGA CAGCAGTCTT CCTCTACTCC 
CTCTATAAGG AGGGTCACTC CAAAGGCCCC TTCTTAGTGA GCGCTCCTCT GTCCACCATC 
ATCAACTGGG AGAGAGAGTT TGAGATGTGG GCTCCTGATA TGTATGTGGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGG GAAAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCGAGGATG AAGAAAGAAG CATCGGTGAA ATTCCATGTT 
CTGCTGACAT CCTATGAGTT GATCACCATT GATATGGCCA TCTTGGGTTC TATTGACTGG 
GCCTGCCTCA TTGTGGATGA AGCCCATCGG CTGAAGAACA ACCAGTCTAA GTTCTTTCGA 
GTTTTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTAA CTGGAACTCC GTTACAGAAC 
AATCTAGAGG AACTGTTTCA TCTGCTCAAC TTTCTCACCC CTGAGAGGTT CCACAACTTA 
GAAGGCTTCT TGGAGGAGTT TGCAGACATT GCCAAAGAGG ACCAGATTAA AAAACTCCAC 
GACATGCTGG GGCCTCATAT GTTGCGGCGG CTCAAAGCTG ACGTCTTCAA GAATATGCCA 
TCCAAGACAG AGCTGATTGT GCGCGTGGAG TTGAGTCCTA TGCAGAAGAA GTACTACAAG 
TATATTCTCA CGCGGAATTT CGAAGCACTT AATGCTCGAG GTGGTGGCAA CCAGGTTTCT 
CTGCTGAACG TGGTGATGGA TCTTAAGAAA TGCTGCAACC ACCCTTATCT CTTCCCTGTG 
GCAGCAATGG AAGCCCCTAA GATGCCTAAT GGTATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AGTTGTTGCT GCTGCAGAAG ATGCTTAAGA ACCTGAAAGA GGGAGGGCAT 
CGTGTGCTCA TCTTCTCCCA GATGACCAAG ATGTTGGACT TGCTAGAGGA TTTTTTAGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGTGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGATC GCTTCAATGC ACCGGGTGCT CAACAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGGATCAA TCTGGCCACT GCGGACACAG TTATTATATA TGACTCTGAC 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCCC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TCTACCGGTT TGTGACCCGT GCATCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AGATGATGCT GACACATTTA GTGGTACGGC CTGGCCTGGG CTCCAAGACA 
GGCTCAATGT CTAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAGCTATTC 
AAGGATGAAG CCACGGATGG AGGAGGAGAC AACAAGGAGG GAGAAGACAG CAGTGTTATC 
CACTATGACG ATAAGGCCAT TGAACGACTG CTGGATCGAA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCTCA GTATGTGGTA 
CGGGAAGAAG AGATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATAAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCACAGG AGGACCGAGG TGTGTGTGGC CGGCCCCGCC CCCCACCCAC GGGCCGTTCC 
ACTAGAGCAG TGGGCCCCGC TCATCTGCCC TCTCTCCCTC CAGATTGGCA GGACGACCAG 
TCCGACAACC AGTCCGATTA CTCAGTGGCC TCAGAGGAAG GTGATGAAGA CTTTGACGAA 
CGGTCAGAAG CTCCCCGCAG GCCCAGTCGC AAGGGCCTGC GGAATGATAA AGATAAGCCA 
TTACCTCCTC TGTTGGCCCG TGTTGGTGGG AATATTGAAG TACTTGGTTT TAATGCTCGT 
CAGCGAAAAG CATTTCTTAA TGCAATTATG CGATATGGCA TGCCACCTCA GGATGCTTTT 
ACCACTCAGT GGCTTGTGAG AGATCTTCGA GGCAAGTCAG AGAAAGAATT TAAGGCTTAT 
GTGTCACTCT TCATGCGACA TTTGTGTGAG CCTGGGGCAG ATGGGGCTGA GACCTTTGCT 
GATGGCGTCC CACGAGAAGG CCTGTCTCGT CAACATGTTC TCACTAGGAT CGGTGTCATG 
TCTCTGATTC GAAAGAAGGT TCAGGAGTTT GAACATGTTA ATGGACGCTG GAGCATGCCC 
GAACTGGCTG AAGTAGAGGA AAACAAAAAA ATGTCTCAGC CAGGTTCACC TTCTCCAAAG 
ACTCCTACAC CTTCCACACC AGGGGACACA CAACCCAATA CTCCTGCGCC TGTGCCACCT 
GCTGAGGATG GGATAAAAAT AGAGGAAAAT AGCCTTAAAG AAGAAGAGAG CACAGAAGGA 
GAGAAGGAAG TTAAATCTAC AGCCCCTGAG GCGACTGTGG AGTGTGCACA GCCCCCTGCC 
CCTGCCCCTG CCACTGCCCC TGCCACTGCC ACTGCCCCTG AGGATGACAA GGCTCCTGCT 
GAGCCTCCTG AGGGGGAGGA AAAAGTGGAA AAGGCAGAGG TGAAGGAGAG AACAGAGGAG 
CCTATGGAGA CAGAGGCCAA AGGTACTACT GAGGTGGAGA AAGTGGAGGA GAAGTCTGCG 
GTAGACCTGA CCCCGATCGT GGTGGAAGAC AAAGAAGAGA AGAAGGAGGA GGAGGAGAAA 
AAGGACGTGA TGCTTCAGAA TGGAGAAACT CCCAAGGATC TTAGTGATGA GAAGCAGAAG 
AAAAACAGCA AACAGCGTTT CATGTTCAAC ATTGCAGATG GTGGTTTTAC TGAGTTGCAC 
TCACTGTGGC AGAATGAAGA GCGGGCAGCC ACAGTCACTA AGAAGACTTA CGAGATCTGG 
CACCGTCGCC ATGATTACTG GCTGCTGGCT GGCATTATAA ACCACGGCTA TGCCCGGTGG 
CAGGACATCC AGAATGACCC ACGGTATGCC ATCCTCAATG AGCCTTTCAA GGGTGAAATG 
AATCGTGGCA ATTTTCTAGA GATCAAGAAT AAATTTTTAG CTCGAAGATT CAAGCTCTTA 
GAACAAGCCC TGGTGATCGA GGAGCAGCTT CGCAGGGCAG CTTACCTGAA CATGTCCGAG 
GACCCCTCTC ACCCGTCTAT GGCCCTTAAC ACCCGCTTCG CTGAGGTGGA GTGTTTGGCA 
GAAAGTCACC AGCACCTGTC CAAGGAGTCG ATGGCAGGGA ATAAGCCGGC CAATGCAGTC 
CTACACAAAG GTCTCCTGAA ACAGCTGGAG GAACTGTTGA GTGACATGAA AGCTGATGTG 
ACGCGGCTCC CAGCCACCAT TGCCCGCATC CCCCCAGTCG CTGTGAGGTT ACAGATGTCA 
GAGCGGAACA TTCTCAGCCG CCTGGCAAAC AGGGCGCCAG AACCTCCCCC ACAGCAGGTA 
GCCCAGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505345.1
CDS	355..6189
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPTGRSTRAVGPAHLPSLPPDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGLLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006505282.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505282.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGCG AGATCATCCT GTGTGATACC 
TGTCCCCGAG CCTACCATAT GGTGTGCCTG GACCCAGACA TGGAGAAGGC CCCGGAGGGC 
AAGTGGAGCT GTCCGCACTG TGAGAAGGAG GGGATCCAGT GGGAAGCTAA GGAGGACAAT 
TCTGAGGGTG AGGAGATTCT GGAAGAAGTC GGGGGGGACC CAGAAGAGGA GGATGACCAT 
CACATGGAAT TCTGTCGCGT CTGCAAGGAC GGCGGGGAGC TCCTGTGCTG TGACACATGC 
CCTTCTTCCT ACCACATCCA CTGCCTGAAC CCCCCGCTGC CAGAGATCCC AAACGGCGAA 
TGGCTCTGTC CCCGCTGTAC TTGTCCAGCT CTTAAGGGCA AAGTGCAGAA GATCCTAATT 
TGGAAGTGGG GCCAGCCACC GTCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGACCCT 
AATACCCCGT CGCCCAAGCC CTTGGAGGGG CGGCCAGAGA GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTGTCAGAAC TGCAGTTGGA GCTGCACTGT 
CAGGTGATGT TCAGAAACTA CCAGCGGAAG AATGATATGG ACGAGCCGCC TTCTGGGGAC 
TTTGGTGGAG ATGAAGAGAA GAGCCGGAAG CGAAAGAACA AGGACCCTAA GTTTGCAGAG 
ATGGAAGAGC GCTTCTACCG CTATGGAATA AAACCGGAGT GGATGATGAT CCACCGCATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTTCACTATT TAATCAAGTG GCGAGACCTG 
CCCTATGACC AGGCGTCCTG GGAGAGCGAG GACGTGGAGA TTCAGGACTA CGACCTGTTC 
AAGCAGAGCT ACTGGAACCA CAGGGAGTTA ATGAGGGGTG AAGAAGGACG ACCCGGCAAG 
AAGCTTAAGA AGGTGAAGCT ACGGAAACTG GAAAGGCCTC CGGAGACCCC AACTGTTGAT 
CCCACCGTGA AGTATGAGCG ACAGCCGGAG TATCTGGATG CGACGGGTGG AACCCTGCAT 
CCCTACCAAA TGGAAGGCTT AAACTGGCTC CGCTTCTCCT GGGCTCAGGG AACCGATACT 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTGCAGA CAGCAGTCTT CCTCTACTCC 
CTCTATAAGG AGGGTCACTC CAAAGGCCCC TTCTTAGTGA GCGCTCCTCT GTCCACCATC 
ATCAACTGGG AGAGAGAGTT TGAGATGTGG GCTCCTGATA TGTATGTGGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGG GAAAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCGAGGATG AAGAAAGAAG CATCGGTGAA ATTCCATGTT 
CTGCTGACAT CCTATGAGTT GATCACCATT GATATGGCCA TCTTGGGTTC TATTGACTGG 
GCCTGCCTCA TTGTGGATGA AGCCCATCGG CTGAAGAACA ACCAGTCTAA GTTCTTTCGA 
GTTTTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTAA CTGGAACTCC GTTACAGAAC 
AATCTAGAGG AACTGTTTCA TCTGCTCAAC TTTCTCACCC CTGAGAGGTT CCACAACTTA 
GAAGGCTTCT TGGAGGAGTT TGCAGACATT GCCAAAGAGG ACCAGATTAA AAAACTCCAC 
GACATGCTGG GGCCTCATAT GTTGCGGCGG CTCAAAGCTG ACGTCTTCAA GAATATGCCA 
TCCAAGACAG AGCTGATTGT GCGCGTGGAG TTGAGTCCTA TGCAGAAGAA GTACTACAAG 
TATATTCTCA CGCGGAATTT CGAAGCACTT AATGCTCGAG GTGGTGGCAA CCAGGTTTCT 
CTGCTGAACG TGGTGATGGA TCTTAAGAAA TGCTGCAACC ACCCTTATCT CTTCCCTGTG 
GCAGCAATGG AAGCCCCTAA GATGCCTAAT GGTATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AGTTGTTGCT GCTGCAGAAG ATGCTTAAGA ACCTGAAAGA GGGAGGGCAT 
CGTGTGCTCA TCTTCTCCCA GATGACCAAG ATGTTGGACT TGCTAGAGGA TTTTTTAGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGTGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGATC GCTTCAATGC ACCGGGTGCT CAACAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGGATCAA TCTGGCCACT GCGGACACAG TTATTATATA TGACTCTGAC 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCCC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TCTACCGGTT TGTGACCCGT GCATCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AGATGATGCT GACACATTTA GTGGTACGGC CTGGCCTGGG CTCCAAGACA 
GGCTCAATGT CTAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAGCTATTC 
AAGGATGAAG CCACGGATGG AGGAGGAGAC AACAAGGAGG GAGAAGACAG CAGTGTTATC 
CACTATGACG ATAAGGCCAT TGAACGACTG CTGGATCGAA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCTCA GTATGTGGTA 
CGGGAAGAAG AGATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATAAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCACAGG AGGACCGAGG TGTGTGTGGC CGGCCCCGCC CCCCACCCAC GGGCCGTTCC 
ACTAGAGCAG TGGGCCCCGC TCATCTGCCC TCTCTCCCTC CAGATTGGCA GGACGACCAG 
TCCGACAACC AGTCCGATTA CTCAGTGGCC TCAGAGGAAG GTGATGAAGA CTTTGACGAA 
CGGTCAGAAG CTCCCCGCAG GCCCAGTCGC AAGGGCCTGC GGAATGATAA AGATAAGCCA 
TTACCTCCTC TGTTGGCCCG TGTTGGTGGG AATATTGAAG TACTTGGTTT TAATGCTCGT 
CAGCGAAAAG CATTTCTTAA TGCAATTATG CGATATGGCA TGCCACCTCA GGATGCTTTT 
ACCACTCAGT GGCTTGTGAG AGATCTTCGA GGCAAGTCAG AGAAAGAATT TAAGGCTTAT 
GTGTCACTCT TCATGCGACA TTTGTGTGAG CCTGGGGCAG ATGGGGCTGA GACCTTTGCT 
GATGGCGTCC CACGAGAAGG CCTGTCTCGT CAACATGTTC TCACTAGGAT CGGTGTCATG 
TCTCTGATTC GAAAGAAGGT TCAGGAGTTT GAACATGTTA ATGGACGCTG GAGCATGCCC 
GAACTGGCTG AAGTAGAGGA AAACAAAAAA ATGTCTCAGC CAGGTTCACC TTCTCCAAAG 
ACTCCTACAC CTTCCACACC AGGGGACACA CAACCCAATA CTCCTGCGCC TGTGCCACCT 
GCTGAGGATG GGATAAAAAT AGAGGAAAAT AGCCTTAAAG AAGAAGAGAG CACAGAAGGA 
GAGAAGGAAG TTAAATCTAC AGCCCCTGAG GCGACTGTGG AGTGTGCACA GCCCCCTGCC 
CCTGCCCCTG CCACTGCCCC TGCCACTGCC ACTGCCCCTG AGGATGACAA GGCTCCTGCT 
GAGCCTCCTG AGGGGGAGGA AAAAGTGGAA AAGGCAGAGG TGAAGGAGAG AACAGAGGAG 
CCTATGGAGA CAGAGGCCAA AGGTACTACT GAGGTGGAGA AAGTGGAGGA GAAGTCTGCG 
GTAGACCTGA CCCCGATCGT GGTGGAAGAC AAAGAAGAGA AGAAGGAGGA GGAGGAGAAA 
AAGGACGTGA TGCTTCAGAA TGGAGAAACT CCCAAGGATC TTAGTGATGA GAAGCAGAAG 
AAAAACAGCA AACAGCGTTT CATGTTCAAC ATTGCAGATG GTGGTTTTAC TGAGTTGCAC 
TCACTGTGGC AGAATGAAGA GCGGGCAGCC ACAGTCACTA AGAAGACTTA CGAGATCTGG 
CACCGTCGCC ATGATTACTG GCTGCTGGCT GGCATTATAA ACCACGGCTA TGCCCGGTGG 
CAGGACATCC AGAATGACCC ACGGTATGCC ATCCTCAATG AGCCTTTCAA GGGTGAAATG 
AATCGTGGCA ATTTTCTAGA GATCAAGAAT AAATTTTTAG CTCGAAGATT CAAGCTCTTA 
GAACAAGCCC TGGTGATCGA GGAGCAGCTT CGCAGGGCAG CTTACCTGAA CATGTCCGAG 
GACCCCTCTC ACCCGTCTAT GGCCCTTAAC ACCCGCTTCG CTGAGGTGGA GTGTTTGGCA 
GAAAGTCACC AGCACCTGTC CAAGGAGTCG ATGGCAGGGA ATAAGCCGGC CAATGCAGTC 
CTACACAAAG GTCTCCTGAA ACAGCTGGAG GAACTGTTGA GTGACATGAA AGCTGATGTG 
ACGCGGCTCC CAGCCACCAT TGCCCGCATC CCCCCAGTCG CTGTGAGGTT ACAGATGTCA 
GAGCGGAACA TTCTCAGCCG CCTGGCAAAC AGGGCGCCAG AACCTCCCCC ACAGCAGGTA 
GCCCAGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_006505283.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505346.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X5, mRNA.

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CloneID	OMu36721
Clone ID Related Accession (Same CDS sequence)	XM_006505283.1
Accession Version	XM_006505283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG CGAGATCATC CTGTGTGATA CCTGTCCCCG AGCCTACCAT 
ATGGTGTGCC TGGACCCAGA CATGGAGAAG GCCCCGGAGG GCAAGTGGAG CTGTCCGCAC 
TGTGAGAAGG AGGGGATCCA GTGGGAAGCT AAGGAGGACA ATTCTGAGGG TGAGGAGATT 
CTGGAAGAAG TCGGGGGGGA CCCAGAAGAG GAGGATGACC ATCACATGGA ATTCTGTCGC 
GTCTGCAAGG ACGGCGGGGA GCTCCTGTGC TGTGACACAT GCCCTTCTTC CTACCACATC 
CACTGCCTGA ACCCCCCGCT GCCAGAGATC CCAAACGGCG AATGGCTCTG TCCCCGCTGT 
ACTTGTCCAG CTCTTAAGGG CAAAGTGCAG AAGATCCTAA TTTGGAAGTG GGGCCAGCCA 
CCGTCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGACC CTAATACCCC GTCGCCCAAG 
CCCTTGGAGG GGCGGCCAGA GAGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTGTCAGA ACTGCAGTTG GAGCTGCACT GTCAGGTGAT GTTCAGAAAC 
TACCAGCGGA AGAATGATAT GGACGAGCCG CCTTCTGGGG ACTTTGGTGG AGATGAAGAG 
AAGAGCCGGA AGCGAAAGAA CAAGGACCCT AAGTTTGCAG AGATGGAAGA GCGCTTCTAC 
CGCTATGGAA TAAAACCGGA GTGGATGATG ATCCACCGCA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTTCACTA TTTAATCAAG TGGCGAGACC TGCCCTATGA CCAGGCGTCC 
TGGGAGAGCG AGGACGTGGA GATTCAGGAC TACGACCTGT TCAAGCAGAG CTACTGGAAC 
CACAGGGAGT TAATGAGGGG TGAAGAAGGA CGACCCGGCA AGAAGCTTAA GAAGGTGAAG 
CTACGGAAAC TGGAAAGGCC TCCGGAGACC CCAACTGTTG ATCCCACCGT GAAGTATGAG 
CGACAGCCGG AGTATCTGGA TGCGACGGGT GGAACCCTGC ATCCCTACCA AATGGAAGGC 
TTAAACTGGC TCCGCTTCTC CTGGGCTCAG GGAACCGATA CTATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTGCA GACAGCAGTC TTCCTCTACT CCCTCTATAA GGAGGGTCAC 
TCCAAAGGCC CCTTCTTAGT GAGCGCTCCT CTGTCCACCA TCATCAACTG GGAGAGAGAG 
TTTGAGATGT GGGCTCCTGA TATGTATGTG GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GGGAAAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCGAGGA TGAAGAAAGA AGCATCGGTG AAATTCCATG TTCTGCTGAC ATCCTATGAG 
TTGATCACCA TTGATATGGC CATCTTGGGT TCTATTGACT GGGCCTGCCT CATTGTGGAT 
GAAGCCCATC GGCTGAAGAA CAACCAGTCT AAGTTCTTTC GAGTTTTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT AACTGGAACT CCGTTACAGA ACAATCTAGA GGAACTGTTT 
CATCTGCTCA ACTTTCTCAC CCCTGAGAGG TTCCACAACT TAGAAGGCTT CTTGGAGGAG 
TTTGCAGACA TTGCCAAAGA GGACCAGATT AAAAAACTCC ACGACATGCT GGGGCCTCAT 
ATGTTGCGGC GGCTCAAAGC TGACGTCTTC AAGAATATGC CATCCAAGAC AGAGCTGATT 
GTGCGCGTGG AGTTGAGTCC TATGCAGAAG AAGTACTACA AGTATATTCT CACGCGGAAT 
TTCGAAGCAC TTAATGCTCG AGGTGGTGGC AACCAGGTTT CTCTGCTGAA CGTGGTGATG 
GATCTTAAGA AATGCTGCAA CCACCCTTAT CTCTTCCCTG TGGCAGCAAT GGAAGCCCCT 
AAGATGCCTA ATGGTATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAGTTGTTG 
CTGCTGCAGA AGATGCTTAA GAACCTGAAA GAGGGAGGGC ATCGTGTGCT CATCTTCTCC 
CAGATGACCA AGATGTTGGA CTTGCTAGAG GATTTTTTAG AACATGAAGG TTATAAATAT 
GAACGTATTG ATGGTGGAAT CACTGGGAAC ATGCGTCAGG AGGCCATTGA TCGCTTCAAT 
GCACCGGGTG CTCAACAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGGATC 
AATCTGGCCA CTGCGGACAC AGTTATTATA TATGACTCTG ACTGGAACCC CCATAATGAC 
ATCCAGGCCT TTAGCAGAGC CCACCGTATT GGGCAAAATA AGAAGGTGAT GATCTACCGG 
TTTGTGACCC GTGCATCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAGATGATG 
CTGACACATT TAGTGGTACG GCCTGGCCTG GGCTCCAAGA CAGGCTCAAT GTCTAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAGCTAT TCAAGGATGA AGCCACGGAT 
GGAGGAGGAG ACAACAAGGA GGGAGAAGAC AGCAGTGTTA TCCACTATGA CGATAAGGCC 
ATTGAACGAC TGCTGGATCG AAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCT CAGTATGTGG TACGGGAAGA AGAGATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATA AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCACA GGAGGACCGA 
GGTGTGTGTG GCCGGCCCCG CCCCCCACCC ACGGGCCGTT CCACTAGAGC AGTGGGCCCC 
GCTCATCTGC CCTCTCTCCC TCCAGATTGG CAGGACGACC AGTCCGACAA CCAGTCCGAT 
TACTCAGTGG CCTCAGAGGA AGGTGATGAA GACTTTGACG AACGGTCAGA AGCTCCCCGC 
AGGCCCAGTC GCAAGGGCCT GCGGAATGAT AAAGATAAGC CATTACCTCC TCTGTTGGCC 
CGTGTTGGTG GGAATATTGA AGTACTTGGT TTTAATGCTC GTCAGCGAAA AGCATTTCTT 
AATGCAATTA TGCGATATGG CATGCCACCT CAGGATGCTT TTACCACTCA GTGGCTTGTG 
AGAGATCTTC GAGGCAAGTC AGAGAAAGAA TTTAAGGCTT ATGTGTCACT CTTCATGCGA 
CATTTGTGTG AGCCTGGGGC AGATGGGGCT GAGACCTTTG CTGATGGCGT CCCACGAGAA 
GGCCTGTCTC GTCAACATGT TCTCACTAGG ATCGGTGTCA TGTCTCTGAT TCGAAAGAAG 
GTTCAGGAGT TTGAACATGT TAATGGACGC TGGAGCATGC CCGAACTGGC TGAAGTAGAG 
GAAAACAAAA AAATGTCTCA GCCAGGTTCA CCTTCTCCAA AGACTCCTAC ACCTTCCACA 
CCAGGGGACA CACAACCCAA TACTCCTGCG CCTGTGCCAC CTGCTGAGGA TGGGATAAAA 
ATAGAGGAAA ATAGCCTTAA AGAAGAAGAG AGCACAGAAG GAGAGAAGGA AGTTAAATCT 
ACAGCCCCTG AGGCGACTGT GGAGTGTGCA CAGCCCCCTG CCCCTGCCCC TGCCACTGCC 
CCTGCCACTG CCACTGCCCC TGAGGATGAC AAGGCTCCTG CTGAGCCTCC TGAGGGGGAG 
GAAAAAGTGG AAAAGGCAGA GGTGAAGGAG AGAACAGAGG AGCCTATGGA GACAGAGGCC 
AAAGGTACTA CTGAGGTGGA GAAAGTGGAG GAGAAGTCTG CGGTAGACCT GACCCCGATC 
GTGGTGGAAG ACAAAGAAGA GAAGAAGGAG GAGGAGGAGA AAAAGGACGT GATGCTTCAG 
AATGGAGAAA CTCCCAAGGA TCTTAGTGAT GAGAAGCAGA AGAAAAACAG CAAACAGCGT 
TTCATGTTCA ACATTGCAGA TGGTGGTTTT ACTGAGTTGC ACTCACTGTG GCAGAATGAA 
GAGCGGGCAG CCACAGTCAC TAAGAAGACT TACGAGATCT GGCACCGTCG CCATGATTAC 
TGGCTGCTGG CTGGCATTAT AAACCACGGC TATGCCCGGT GGCAGGACAT CCAGAATGAC 
CCACGGTATG CCATCCTCAA TGAGCCTTTC AAGGGTGAAA TGAATCGTGG CAATTTTCTA 
GAGATCAAGA ATAAATTTTT AGCTCGAAGA TTCAAGCTCT TAGAACAAGC CCTGGTGATC 
GAGGAGCAGC TTCGCAGGGC AGCTTACCTG AACATGTCCG AGGACCCCTC TCACCCGTCT 
ATGGCCCTTA ACACCCGCTT CGCTGAGGTG GAGTGTTTGG CAGAAAGTCA CCAGCACCTG 
TCCAAGGAGT CGATGGCAGG GAATAAGCCG GCCAATGCAG TCCTACACAA AGGTCTCCTG 
AAACAGCTGG AGGAACTGTT GAGTGACATG AAAGCTGATG TGACGCGGCT CCCAGCCACC 
ATTGCCCGCA TCCCCCCAGT CGCTGTGAGG TTACAGATGT CAGAGCGGAA CATTCTCAGC 
CGCCTGGCAA ACAGGGCGCC AGAACCTCCC CCACAGCAGG TAGCCCAGCA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505346.1
CDS	355..6171
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPTGRSTRAVGPAHLPSLPPDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGLLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006505283.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505283.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG CGAGATCATC CTGTGTGATA CCTGTCCCCG AGCCTACCAT 
ATGGTGTGCC TGGACCCAGA CATGGAGAAG GCCCCGGAGG GCAAGTGGAG CTGTCCGCAC 
TGTGAGAAGG AGGGGATCCA GTGGGAAGCT AAGGAGGACA ATTCTGAGGG TGAGGAGATT 
CTGGAAGAAG TCGGGGGGGA CCCAGAAGAG GAGGATGACC ATCACATGGA ATTCTGTCGC 
GTCTGCAAGG ACGGCGGGGA GCTCCTGTGC TGTGACACAT GCCCTTCTTC CTACCACATC 
CACTGCCTGA ACCCCCCGCT GCCAGAGATC CCAAACGGCG AATGGCTCTG TCCCCGCTGT 
ACTTGTCCAG CTCTTAAGGG CAAAGTGCAG AAGATCCTAA TTTGGAAGTG GGGCCAGCCA 
CCGTCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGACC CTAATACCCC GTCGCCCAAG 
CCCTTGGAGG GGCGGCCAGA GAGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTGTCAGA ACTGCAGTTG GAGCTGCACT GTCAGGTGAT GTTCAGAAAC 
TACCAGCGGA AGAATGATAT GGACGAGCCG CCTTCTGGGG ACTTTGGTGG AGATGAAGAG 
AAGAGCCGGA AGCGAAAGAA CAAGGACCCT AAGTTTGCAG AGATGGAAGA GCGCTTCTAC 
CGCTATGGAA TAAAACCGGA GTGGATGATG ATCCACCGCA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTTCACTA TTTAATCAAG TGGCGAGACC TGCCCTATGA CCAGGCGTCC 
TGGGAGAGCG AGGACGTGGA GATTCAGGAC TACGACCTGT TCAAGCAGAG CTACTGGAAC 
CACAGGGAGT TAATGAGGGG TGAAGAAGGA CGACCCGGCA AGAAGCTTAA GAAGGTGAAG 
CTACGGAAAC TGGAAAGGCC TCCGGAGACC CCAACTGTTG ATCCCACCGT GAAGTATGAG 
CGACAGCCGG AGTATCTGGA TGCGACGGGT GGAACCCTGC ATCCCTACCA AATGGAAGGC 
TTAAACTGGC TCCGCTTCTC CTGGGCTCAG GGAACCGATA CTATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTGCA GACAGCAGTC TTCCTCTACT CCCTCTATAA GGAGGGTCAC 
TCCAAAGGCC CCTTCTTAGT GAGCGCTCCT CTGTCCACCA TCATCAACTG GGAGAGAGAG 
TTTGAGATGT GGGCTCCTGA TATGTATGTG GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GGGAAAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCGAGGA TGAAGAAAGA AGCATCGGTG AAATTCCATG TTCTGCTGAC ATCCTATGAG 
TTGATCACCA TTGATATGGC CATCTTGGGT TCTATTGACT GGGCCTGCCT CATTGTGGAT 
GAAGCCCATC GGCTGAAGAA CAACCAGTCT AAGTTCTTTC GAGTTTTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT AACTGGAACT CCGTTACAGA ACAATCTAGA GGAACTGTTT 
CATCTGCTCA ACTTTCTCAC CCCTGAGAGG TTCCACAACT TAGAAGGCTT CTTGGAGGAG 
TTTGCAGACA TTGCCAAAGA GGACCAGATT AAAAAACTCC ACGACATGCT GGGGCCTCAT 
ATGTTGCGGC GGCTCAAAGC TGACGTCTTC AAGAATATGC CATCCAAGAC AGAGCTGATT 
GTGCGCGTGG AGTTGAGTCC TATGCAGAAG AAGTACTACA AGTATATTCT CACGCGGAAT 
TTCGAAGCAC TTAATGCTCG AGGTGGTGGC AACCAGGTTT CTCTGCTGAA CGTGGTGATG 
GATCTTAAGA AATGCTGCAA CCACCCTTAT CTCTTCCCTG TGGCAGCAAT GGAAGCCCCT 
AAGATGCCTA ATGGTATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAGTTGTTG 
CTGCTGCAGA AGATGCTTAA GAACCTGAAA GAGGGAGGGC ATCGTGTGCT CATCTTCTCC 
CAGATGACCA AGATGTTGGA CTTGCTAGAG GATTTTTTAG AACATGAAGG TTATAAATAT 
GAACGTATTG ATGGTGGAAT CACTGGGAAC ATGCGTCAGG AGGCCATTGA TCGCTTCAAT 
GCACCGGGTG CTCAACAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGGATC 
AATCTGGCCA CTGCGGACAC AGTTATTATA TATGACTCTG ACTGGAACCC CCATAATGAC 
ATCCAGGCCT TTAGCAGAGC CCACCGTATT GGGCAAAATA AGAAGGTGAT GATCTACCGG 
TTTGTGACCC GTGCATCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAGATGATG 
CTGACACATT TAGTGGTACG GCCTGGCCTG GGCTCCAAGA CAGGCTCAAT GTCTAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAGCTAT TCAAGGATGA AGCCACGGAT 
GGAGGAGGAG ACAACAAGGA GGGAGAAGAC AGCAGTGTTA TCCACTATGA CGATAAGGCC 
ATTGAACGAC TGCTGGATCG AAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCT CAGTATGTGG TACGGGAAGA AGAGATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATA AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCACA GGAGGACCGA 
GGTGTGTGTG GCCGGCCCCG CCCCCCACCC ACGGGCCGTT CCACTAGAGC AGTGGGCCCC 
GCTCATCTGC CCTCTCTCCC TCCAGATTGG CAGGACGACC AGTCCGACAA CCAGTCCGAT 
TACTCAGTGG CCTCAGAGGA AGGTGATGAA GACTTTGACG AACGGTCAGA AGCTCCCCGC 
AGGCCCAGTC GCAAGGGCCT GCGGAATGAT AAAGATAAGC CATTACCTCC TCTGTTGGCC 
CGTGTTGGTG GGAATATTGA AGTACTTGGT TTTAATGCTC GTCAGCGAAA AGCATTTCTT 
AATGCAATTA TGCGATATGG CATGCCACCT CAGGATGCTT TTACCACTCA GTGGCTTGTG 
AGAGATCTTC GAGGCAAGTC AGAGAAAGAA TTTAAGGCTT ATGTGTCACT CTTCATGCGA 
CATTTGTGTG AGCCTGGGGC AGATGGGGCT GAGACCTTTG CTGATGGCGT CCCACGAGAA 
GGCCTGTCTC GTCAACATGT TCTCACTAGG ATCGGTGTCA TGTCTCTGAT TCGAAAGAAG 
GTTCAGGAGT TTGAACATGT TAATGGACGC TGGAGCATGC CCGAACTGGC TGAAGTAGAG 
GAAAACAAAA AAATGTCTCA GCCAGGTTCA CCTTCTCCAA AGACTCCTAC ACCTTCCACA 
CCAGGGGACA CACAACCCAA TACTCCTGCG CCTGTGCCAC CTGCTGAGGA TGGGATAAAA 
ATAGAGGAAA ATAGCCTTAA AGAAGAAGAG AGCACAGAAG GAGAGAAGGA AGTTAAATCT 
ACAGCCCCTG AGGCGACTGT GGAGTGTGCA CAGCCCCCTG CCCCTGCCCC TGCCACTGCC 
CCTGCCACTG CCACTGCCCC TGAGGATGAC AAGGCTCCTG CTGAGCCTCC TGAGGGGGAG 
GAAAAAGTGG AAAAGGCAGA GGTGAAGGAG AGAACAGAGG AGCCTATGGA GACAGAGGCC 
AAAGGTACTA CTGAGGTGGA GAAAGTGGAG GAGAAGTCTG CGGTAGACCT GACCCCGATC 
GTGGTGGAAG ACAAAGAAGA GAAGAAGGAG GAGGAGGAGA AAAAGGACGT GATGCTTCAG 
AATGGAGAAA CTCCCAAGGA TCTTAGTGAT GAGAAGCAGA AGAAAAACAG CAAACAGCGT 
TTCATGTTCA ACATTGCAGA TGGTGGTTTT ACTGAGTTGC ACTCACTGTG GCAGAATGAA 
GAGCGGGCAG CCACAGTCAC TAAGAAGACT TACGAGATCT GGCACCGTCG CCATGATTAC 
TGGCTGCTGG CTGGCATTAT AAACCACGGC TATGCCCGGT GGCAGGACAT CCAGAATGAC 
CCACGGTATG CCATCCTCAA TGAGCCTTTC AAGGGTGAAA TGAATCGTGG CAATTTTCTA 
GAGATCAAGA ATAAATTTTT AGCTCGAAGA TTCAAGCTCT TAGAACAAGC CCTGGTGATC 
GAGGAGCAGC TTCGCAGGGC AGCTTACCTG AACATGTCCG AGGACCCCTC TCACCCGTCT 
ATGGCCCTTA ACACCCGCTT CGCTGAGGTG GAGTGTTTGG CAGAAAGTCA CCAGCACCTG 
TCCAAGGAGT CGATGGCAGG GAATAAGCCG GCCAATGCAG TCCTACACAA AGGTCTCCTG 
AAACAGCTGG AGGAACTGTT GAGTGACATG AAAGCTGATG TGACGCGGCT CCCAGCCACC 
ATTGCCCGCA TCCCCCCAGT CGCTGTGAGG TTACAGATGT CAGAGCGGAA CATTCTCAGC 
CGCCTGGCAA ACAGGGCGCC AGAACCTCCC CCACAGCAGG TAGCCCAGCA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_006505284.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505347.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X6, mRNA.

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CloneID	OMu36722
Clone ID Related Accession (Same CDS sequence)	XM_006505284.1
Accession Version	XM_006505284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA CGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ACTCAGTGGC CTCAGAGGAA 
GGTGATGAAG ACTTTGACGA ACGGTCAGAA GCTCCCCGCA GGCCCAGTCG CAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTACCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTACTTGGTT TTAATGCTCG TCAGCGAAAA GCATTTCTTA ATGCAATTAT GCGATATGGC 
ATGCCACCTC AGGATGCTTT TACCACTCAG TGGCTTGTGA GAGATCTTCG AGGCAAGTCA 
GAGAAAGAAT TTAAGGCTTA TGTGTCACTC TTCATGCGAC ATTTGTGTGA GCCTGGGGCA 
GATGGGGCTG AGACCTTTGC TGATGGCGTC CCACGAGAAG GCCTGTCTCG TCAACATGTT 
CTCACTAGGA TCGGTGTCAT GTCTCTGATT CGAAAGAAGG TTCAGGAGTT TGAACATGTT 
AATGGACGCT GGAGCATGCC CGAACTGGCT GAAGTAGAGG AAAACAAAAA AATGTCTCAG 
CCAGGTTCAC CTTCTCCAAA GACTCCTACA CCTTCCACAC CAGGGGACAC ACAACCCAAT 
ACTCCTGCGC CTGTGCCACC TGCTGAGGAT GGGATAAAAA TAGAGGAAAA TAGCCTTAAA 
GAAGAAGAGA GCACAGAAGG AGAGAAGGAA GTTAAATCTA CAGCCCCTGA GGCGACTGTG 
GAGTGTGCAC AGCCCCCTGC CCCTGCCCCT GCCACTGCCC CTGCCACTGC CACTGCCCCT 
GAGGATGACA AGGCTCCTGC TGAGCCTCCT GAGGGGGAGG AAAAAGTGGA AAAGGCAGAG 
GTGAAGGAGA GAACAGAGGA GCCTATGGAG ACAGAGGCCA AAGGTACTAC TGAGGTGGAG 
AAAGTGGAGG AGAAGTCTGC GGTAGACCTG ACCCCGATCG TGGTGGAAGA CAAAGAAGAG 
AAGAAGGAGG AGGAGGAGAA AAAGGACGTG ATGCTTCAGA ATGGAGAAAC TCCCAAGGAT 
CTTAGTGATG AGAAGCAGAA GAAAAACAGC AAACAGCGTT TCATGTTCAA CATTGCAGAT 
GGTGGTTTTA CTGAGTTGCA CTCACTGTGG CAGAATGAAG AGCGGGCAGC CACAGTCACT 
AAGAAGACTT ACGAGATCTG GCACCGTCGC CATGATTACT GGCTGCTGGC TGGCATTATA 
AACCACGGCT ATGCCCGGTG GCAGGACATC CAGAATGACC CACGGTATGC CATCCTCAAT 
GAGCCTTTCA AGGGTGAAAT GAATCGTGGC AATTTTCTAG AGATCAAGAA TAAATTTTTA 
GCTCGAAGAT TCAAGCTCTT AGAACAAGCC CTGGTGATCG AGGAGCAGCT TCGCAGGGCA 
GCTTACCTGA ACATGTCCGA GGACCCCTCT CACCCGTCTA TGGCCCTTAA CACCCGCTTC 
GCTGAGGTGG AGTGTTTGGC AGAAAGTCAC CAGCACCTGT CCAAGGAGTC GATGGCAGGG 
AATAAGCCGG CCAATGCAGT CCTACACAAA GGTCTCCTGA AACAGCTGGA GGAACTGTTG 
AGTGACATGA AAGCTGATGT GACGCGGCTC CCAGCCACCA TTGCCCGCAT CCCCCCAGTC 
GCTGTGAGGT TACAGATGTC AGAGCGGAAC ATTCTCAGCC GCCTGGCAAA CAGGGCGCCA 
GAACCTCCCC CACAGCAGGT AGCCCAGCAG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505347.1
CDS	355..6150
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPTGRSTRAVGPAHLPSLPPDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGLLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006505284.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505284.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA CGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ACTCAGTGGC CTCAGAGGAA 
GGTGATGAAG ACTTTGACGA ACGGTCAGAA GCTCCCCGCA GGCCCAGTCG CAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTACCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTACTTGGTT TTAATGCTCG TCAGCGAAAA GCATTTCTTA ATGCAATTAT GCGATATGGC 
ATGCCACCTC AGGATGCTTT TACCACTCAG TGGCTTGTGA GAGATCTTCG AGGCAAGTCA 
GAGAAAGAAT TTAAGGCTTA TGTGTCACTC TTCATGCGAC ATTTGTGTGA GCCTGGGGCA 
GATGGGGCTG AGACCTTTGC TGATGGCGTC CCACGAGAAG GCCTGTCTCG TCAACATGTT 
CTCACTAGGA TCGGTGTCAT GTCTCTGATT CGAAAGAAGG TTCAGGAGTT TGAACATGTT 
AATGGACGCT GGAGCATGCC CGAACTGGCT GAAGTAGAGG AAAACAAAAA AATGTCTCAG 
CCAGGTTCAC CTTCTCCAAA GACTCCTACA CCTTCCACAC CAGGGGACAC ACAACCCAAT 
ACTCCTGCGC CTGTGCCACC TGCTGAGGAT GGGATAAAAA TAGAGGAAAA TAGCCTTAAA 
GAAGAAGAGA GCACAGAAGG AGAGAAGGAA GTTAAATCTA CAGCCCCTGA GGCGACTGTG 
GAGTGTGCAC AGCCCCCTGC CCCTGCCCCT GCCACTGCCC CTGCCACTGC CACTGCCCCT 
GAGGATGACA AGGCTCCTGC TGAGCCTCCT GAGGGGGAGG AAAAAGTGGA AAAGGCAGAG 
GTGAAGGAGA GAACAGAGGA GCCTATGGAG ACAGAGGCCA AAGGTACTAC TGAGGTGGAG 
AAAGTGGAGG AGAAGTCTGC GGTAGACCTG ACCCCGATCG TGGTGGAAGA CAAAGAAGAG 
AAGAAGGAGG AGGAGGAGAA AAAGGACGTG ATGCTTCAGA ATGGAGAAAC TCCCAAGGAT 
CTTAGTGATG AGAAGCAGAA GAAAAACAGC AAACAGCGTT TCATGTTCAA CATTGCAGAT 
GGTGGTTTTA CTGAGTTGCA CTCACTGTGG CAGAATGAAG AGCGGGCAGC CACAGTCACT 
AAGAAGACTT ACGAGATCTG GCACCGTCGC CATGATTACT GGCTGCTGGC TGGCATTATA 
AACCACGGCT ATGCCCGGTG GCAGGACATC CAGAATGACC CACGGTATGC CATCCTCAAT 
GAGCCTTTCA AGGGTGAAAT GAATCGTGGC AATTTTCTAG AGATCAAGAA TAAATTTTTA 
GCTCGAAGAT TCAAGCTCTT AGAACAAGCC CTGGTGATCG AGGAGCAGCT TCGCAGGGCA 
GCTTACCTGA ACATGTCCGA GGACCCCTCT CACCCGTCTA TGGCCCTTAA CACCCGCTTC 
GCTGAGGTGG AGTGTTTGGC AGAAAGTCAC CAGCACCTGT CCAAGGAGTC GATGGCAGGG 
AATAAGCCGG CCAATGCAGT CCTACACAAA GGTCTCCTGA AACAGCTGGA GGAACTGTTG 
AGTGACATGA AAGCTGATGT GACGCGGCTC CCAGCCACCA TTGCCCGCAT CCCCCCAGTC 
GCTGTGAGGT TACAGATGTC AGAGCGGAAC ATTCTCAGCC GCCTGGCAAA CAGGGCGCCA 
GAACCTCCCC CACAGCAGGT AGCCCAGCAG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_006505285.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505348.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X2, mRNA.

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CloneID	OMu36723
Clone ID Related Accession (Same CDS sequence)	XM_006505285.1
Accession Version	XM_006505285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGG 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTA 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCAT TTCTTAATGC AATTATGCGA TATGGCATGC CACCTCAGGA TGCTTTTACC 
ACTCAGTGGC TTGTGAGAGA TCTTCGAGGC AAGTCAGAGA AAGAATTTAA GGCTTATGTG 
TCACTCTTCA TGCGACATTT GTGTGAGCCT GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGCGTCCCAC GAGAAGGCCT GTCTCGTCAA CATGTTCTCA CTAGGATCGG TGTCATGTCT 
CTGATTCGAA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GACGCTGGAG CATGCCCGAA 
CTGGCTGAAG TAGAGGAAAA CAAAAAAATG TCTCAGCCAG GTTCACCTTC TCCAAAGACT 
CCTACACCTT CCACACCAGG GGACACACAA CCCAATACTC CTGCGCCTGT GCCACCTGCT 
GAGGATGGGA TAAAAATAGA GGAAAATAGC CTTAAAGAAG AAGAGAGCAC AGAAGGAGAG 
AAGGAAGTTA AATCTACAGC CCCTGAGGCG ACTGTGGAGT GTGCACAGCC CCCTGCCCCT 
GCCCCTGCCA CTGCCCCTGC CACTGCCACT GCCCCTGAGG ATGACAAGGC TCCTGCTGAG 
CCTCCTGAGG GGGAGGAAAA AGTGGAAAAG GCAGAGGTGA AGGAGAGAAC AGAGGAGCCT 
ATGGAGACAG AGGCCAAAGG TACTACTGAG GTGGAGAAAG TGGAGGAGAA GTCTGCGGTA 
GACCTGACCC CGATCGTGGT GGAAGACAAA GAAGAGAAGA AGGAGGAGGA GGAGAAAAAG 
GACGTGATGC TTCAGAATGG AGAAACTCCC AAGGATCTTA GTGATGAGAA GCAGAAGAAA 
AACAGCAAAC AGCGTTTCAT GTTCAACATT GCAGATGGTG GTTTTACTGA GTTGCACTCA 
CTGTGGCAGA ATGAAGAGCG GGCAGCCACA GTCACTAAGA AGACTTACGA GATCTGGCAC 
CGTCGCCATG ATTACTGGCT GCTGGCTGGC ATTATAAACC ACGGCTATGC CCGGTGGCAG 
GACATCCAGA ATGACCCACG GTATGCCATC CTCAATGAGC CTTTCAAGGG TGAAATGAAT 
CGTGGCAATT TTCTAGAGAT CAAGAATAAA TTTTTAGCTC GAAGATTCAA GCTCTTAGAA 
CAAGCCCTGG TGATCGAGGA GCAGCTTCGC AGGGCAGCTT ACCTGAACAT GTCCGAGGAC 
CCCTCTCACC CGTCTATGGC CCTTAACACC CGCTTCGCTG AGGTGGAGTG TTTGGCAGAA 
AGTCACCAGC ACCTGTCCAA GGAGTCGATG GCAGGGAATA AGCCGGCCAA TGCAGTCCTA 
CACAAAGGTC TCCTGAAACA GCTGGAGGAA CTGTTGAGTG ACATGAAAGC TGATGTGACG 
CGGCTCCCAG CCACCATTGC CCGCATCCCC CCAGTCGCTG TGAGGTTACA GATGTCAGAG 
CGGAACATTC TCAGCCGCCT GGCAAACAGG GCGCCAGAAC CTCCCCCACA GCAGGTAGCC 
CAGCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505348.1
CDS	355..6126
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGLLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006505285.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505285.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGG 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTA 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCAT TTCTTAATGC AATTATGCGA TATGGCATGC CACCTCAGGA TGCTTTTACC 
ACTCAGTGGC TTGTGAGAGA TCTTCGAGGC AAGTCAGAGA AAGAATTTAA GGCTTATGTG 
TCACTCTTCA TGCGACATTT GTGTGAGCCT GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGCGTCCCAC GAGAAGGCCT GTCTCGTCAA CATGTTCTCA CTAGGATCGG TGTCATGTCT 
CTGATTCGAA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GACGCTGGAG CATGCCCGAA 
CTGGCTGAAG TAGAGGAAAA CAAAAAAATG TCTCAGCCAG GTTCACCTTC TCCAAAGACT 
CCTACACCTT CCACACCAGG GGACACACAA CCCAATACTC CTGCGCCTGT GCCACCTGCT 
GAGGATGGGA TAAAAATAGA GGAAAATAGC CTTAAAGAAG AAGAGAGCAC AGAAGGAGAG 
AAGGAAGTTA AATCTACAGC CCCTGAGGCG ACTGTGGAGT GTGCACAGCC CCCTGCCCCT 
GCCCCTGCCA CTGCCCCTGC CACTGCCACT GCCCCTGAGG ATGACAAGGC TCCTGCTGAG 
CCTCCTGAGG GGGAGGAAAA AGTGGAAAAG GCAGAGGTGA AGGAGAGAAC AGAGGAGCCT 
ATGGAGACAG AGGCCAAAGG TACTACTGAG GTGGAGAAAG TGGAGGAGAA GTCTGCGGTA 
GACCTGACCC CGATCGTGGT GGAAGACAAA GAAGAGAAGA AGGAGGAGGA GGAGAAAAAG 
GACGTGATGC TTCAGAATGG AGAAACTCCC AAGGATCTTA GTGATGAGAA GCAGAAGAAA 
AACAGCAAAC AGCGTTTCAT GTTCAACATT GCAGATGGTG GTTTTACTGA GTTGCACTCA 
CTGTGGCAGA ATGAAGAGCG GGCAGCCACA GTCACTAAGA AGACTTACGA GATCTGGCAC 
CGTCGCCATG ATTACTGGCT GCTGGCTGGC ATTATAAACC ACGGCTATGC CCGGTGGCAG 
GACATCCAGA ATGACCCACG GTATGCCATC CTCAATGAGC CTTTCAAGGG TGAAATGAAT 
CGTGGCAATT TTCTAGAGAT CAAGAATAAA TTTTTAGCTC GAAGATTCAA GCTCTTAGAA 
CAAGCCCTGG TGATCGAGGA GCAGCTTCGC AGGGCAGCTT ACCTGAACAT GTCCGAGGAC 
CCCTCTCACC CGTCTATGGC CCTTAACACC CGCTTCGCTG AGGTGGAGTG TTTGGCAGAA 
AGTCACCAGC ACCTGTCCAA GGAGTCGATG GCAGGGAATA AGCCGGCCAA TGCAGTCCTA 
CACAAAGGTC TCCTGAAACA GCTGGAGGAA CTGTTGAGTG ACATGAAAGC TGATGTGACG 
CGGCTCCCAG CCACCATTGC CCGCATCCCC CCAGTCGCTG TGAGGTTACA GATGTCAGAG 
CGGAACATTC TCAGCCGCCT GGCAAACAGG GCGCCAGAAC CTCCCCCACA GCAGGTAGCC 
CAGCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_006505286.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505349.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X3, mRNA.

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CloneID	OMu36724
Clone ID Related Accession (Same CDS sequence)	XM_006505286.1
Accession Version	XM_006505286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGG 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTA 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCAT TTCTTAATGC AATTATGCGA TATGGCATGC CACCTCAGGA TGCTTTTACC 
ACTCAGTGGC TTGTGAGAGA TCTTCGAGGC AAGTCAGAGA AAGAATTTAA GGCTTATGTG 
TCACTCTTCA TGCGACATTT GTGTGAGCCT GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGCGTCCCAC GAGAAGGCCT GTCTCGTCAA CATGTTCTCA CTAGGATCGG TGTCATGTCT 
CTGATTCGAA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GACGCTGGAG CATGCCCGAA 
CTGGCTGAAG TAGAGGAAAA CAAAAAAATG TCTCAGCCAG GTTCACCTTC TCCAAAGACT 
CCTACACCTT CCACACCAGG GGACACACAA CCCAATACTC CTGCGCCTGT GCCACCTGCT 
GAGGATGGGA TAAAAATAGA GGAAAATAGC CTTAAAGAAG AAGAGAGCAC AGAAGGAGAG 
AAGGAAGTTA AATCTACAGC CCCTGAGGCG ACTGTGGAGT GTGCACAGCC CCCTGCCCCT 
GCCCCTGCCA CTGCCCCTGC CACTGCCACT GCCCCTGAGG ATGACAAGGC TCCTGCTGAG 
CCTCCTGAGG GGGAGGAAAA AGTGGAAAAG GCAGAGGTGA AGGAGAGAAC AGAGGAGCCT 
ATGGAGACAG AGGCCAAAGG TACTACTGAG GTGGAGAAAG TGGAGGAGAA GTCTGCGGTA 
GACCTGACCC CGATCGTGGT GGAAGACAAA GAAGAGAAGA AGGAGGAGGA GGAGAAAAAG 
GACGTGATGC TTCAGAATGG AGAAACTCCC AAGGATCTTA GTGATGAGAA GCAGAAGAAA 
AACAGCAAAC AGCGTTTCAT GTTCAACATT GCAGATGGTG GTTTTACTGA GTTGCACTCA 
CTGTGGCAGA ATGAAGAGCG GGCAGCCACA GTCACTAAGA AGACTTACGA GATCTGGCAC 
CGTCGCCATG ATTACTGGCT GCTGGCTGGC ATTATAAACC ACGGCTATGC CCGGTGGCAG 
GACATCCAGA ATGACCCACG GTATGCCATC CTCAATGAGC CTTTCAAGGG TGAAATGAAT 
CGTGGCAATT TTCTAGAGAT CAAGAATAAA TTTTTAGCTC GAAGATTCAA GCTCTTAGAA 
CAAGCCCTGG TGATCGAGGA GCAGCTTCGC AGGGCAGCTT ACCTGAACAT GTCCGAGGAC 
CCCTCTCACC CGTCTATGGC CCTTAACACC CGCTTCGCTG AGGTGGAGTG TTTGGCAGAA 
AGTCACCAGC ACCTGTCCAA GGAGTCGATG GCAGGGAATA AGCCGGCCAA TGCAGTCCTA 
CACAAAGTCC TGAAACAGCT GGAGGAACTG TTGAGTGACA TGAAAGCTGA TGTGACGCGG 
CTCCCAGCCA CCATTGCCCG CATCCCCCCA GTCGCTGTGA GGTTACAGAT GTCAGAGCGG 
AACATTCTCA GCCGCCTGGC AAACAGGGCG CCAGAACCTC CCCCACAGCA GGTAGCCCAG 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505349.1
CDS	355..6123
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006505286.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505286.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGG 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTA 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCAT TTCTTAATGC AATTATGCGA TATGGCATGC CACCTCAGGA TGCTTTTACC 
ACTCAGTGGC TTGTGAGAGA TCTTCGAGGC AAGTCAGAGA AAGAATTTAA GGCTTATGTG 
TCACTCTTCA TGCGACATTT GTGTGAGCCT GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGCGTCCCAC GAGAAGGCCT GTCTCGTCAA CATGTTCTCA CTAGGATCGG TGTCATGTCT 
CTGATTCGAA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GACGCTGGAG CATGCCCGAA 
CTGGCTGAAG TAGAGGAAAA CAAAAAAATG TCTCAGCCAG GTTCACCTTC TCCAAAGACT 
CCTACACCTT CCACACCAGG GGACACACAA CCCAATACTC CTGCGCCTGT GCCACCTGCT 
GAGGATGGGA TAAAAATAGA GGAAAATAGC CTTAAAGAAG AAGAGAGCAC AGAAGGAGAG 
AAGGAAGTTA AATCTACAGC CCCTGAGGCG ACTGTGGAGT GTGCACAGCC CCCTGCCCCT 
GCCCCTGCCA CTGCCCCTGC CACTGCCACT GCCCCTGAGG ATGACAAGGC TCCTGCTGAG 
CCTCCTGAGG GGGAGGAAAA AGTGGAAAAG GCAGAGGTGA AGGAGAGAAC AGAGGAGCCT 
ATGGAGACAG AGGCCAAAGG TACTACTGAG GTGGAGAAAG TGGAGGAGAA GTCTGCGGTA 
GACCTGACCC CGATCGTGGT GGAAGACAAA GAAGAGAAGA AGGAGGAGGA GGAGAAAAAG 
GACGTGATGC TTCAGAATGG AGAAACTCCC AAGGATCTTA GTGATGAGAA GCAGAAGAAA 
AACAGCAAAC AGCGTTTCAT GTTCAACATT GCAGATGGTG GTTTTACTGA GTTGCACTCA 
CTGTGGCAGA ATGAAGAGCG GGCAGCCACA GTCACTAAGA AGACTTACGA GATCTGGCAC 
CGTCGCCATG ATTACTGGCT GCTGGCTGGC ATTATAAACC ACGGCTATGC CCGGTGGCAG 
GACATCCAGA ATGACCCACG GTATGCCATC CTCAATGAGC CTTTCAAGGG TGAAATGAAT 
CGTGGCAATT TTCTAGAGAT CAAGAATAAA TTTTTAGCTC GAAGATTCAA GCTCTTAGAA 
CAAGCCCTGG TGATCGAGGA GCAGCTTCGC AGGGCAGCTT ACCTGAACAT GTCCGAGGAC 
CCCTCTCACC CGTCTATGGC CCTTAACACC CGCTTCGCTG AGGTGGAGTG TTTGGCAGAA 
AGTCACCAGC ACCTGTCCAA GGAGTCGATG GCAGGGAATA AGCCGGCCAA TGCAGTCCTA 
CACAAAGTCC TGAAACAGCT GGAGGAACTG TTGAGTGACA TGAAAGCTGA TGTGACGCGG 
CTCCCAGCCA CCATTGCCCG CATCCCCCCA GTCGCTGTGA GGTTACAGAT GTCAGAGCGG 
AACATTCTCA GCCGCCTGGC AAACAGGGCG CCAGAACCTC CCCCACAGCA GGTAGCCCAG 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_006505287.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505350.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X4, mRNA.

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CloneID	OMu36725
Clone ID Related Accession (Same CDS sequence)	XM_006505287.1
Accession Version	XM_006505287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AAGAGGAGGT AGAACGGGAA 
ATCATAAAAC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTAGCCCGA AATCTGGGCA AAGGAAAAAG AATCCGTAAA 
CAGGTCAACT ACAATGATGG CTCACAGGAG GACCGAGATT GGCAGGACGA CCAGTCCGAC 
AACCAGTCCG ATTACTCAGT GGCCTCAGAG GAAGGTGATG AAGACTTTGA CGAACGGTCA 
GAAGCTCCCC GCAGGCCCAG TCGCAAGGGC CTGCGGAATG ATAAAGATAA GCCATTACCT 
CCTCTGTTGG CCCGTGTTGG TGGGAATATT GAAGTACTTG GTTTTAATGC TCGTCAGCGA 
AAAGCATTTC TTAATGCAAT TATGCGATAT GGCATGCCAC CTCAGGATGC TTTTACCACT 
CAGTGGCTTG TGAGAGATCT TCGAGGCAAG TCAGAGAAAG AATTTAAGGC TTATGTGTCA 
CTCTTCATGC GACATTTGTG TGAGCCTGGG GCAGATGGGG CTGAGACCTT TGCTGATGGC 
GTCCCACGAG AAGGCCTGTC TCGTCAACAT GTTCTCACTA GGATCGGTGT CATGTCTCTG 
ATTCGAAAGA AGGTTCAGGA GTTTGAACAT GTTAATGGAC GCTGGAGCAT GCCCGAACTG 
GCTGAAGTAG AGGAAAACAA AAAAATGTCT CAGCCAGGTT CACCTTCTCC AAAGACTCCT 
ACACCTTCCA CACCAGGGGA CACACAACCC AATACTCCTG CGCCTGTGCC ACCTGCTGAG 
GATGGGATAA AAATAGAGGA AAATAGCCTT AAAGAAGAAG AGAGCACAGA AGGAGAGAAG 
GAAGTTAAAT CTACAGCCCC TGAGGCGACT GTGGAGTGTG CACAGCCCCC TGCCCCTGCC 
CCTGCCACTG CCCCTGCCAC TGCCACTGCC CCTGAGGATG ACAAGGCTCC TGCTGAGCCT 
CCTGAGGGGG AGGAAAAAGT GGAAAAGGCA GAGGTGAAGG AGAGAACAGA GGAGCCTATG 
GAGACAGAGG CCAAAGGTAC TACTGAGGTG GAGAAAGTGG AGGAGAAGTC TGCGGTAGAC 
CTGACCCCGA TCGTGGTGGA AGACAAAGAA GAGAAGAAGG AGGAGGAGGA GAAAAAGGAC 
GTGATGCTTC AGAATGGAGA AACTCCCAAG GATCTTAGTG ATGAGAAGCA GAAGAAAAAC 
AGCAAACAGC GTTTCATGTT CAACATTGCA GATGGTGGTT TTACTGAGTT GCACTCACTG 
TGGCAGAATG AAGAGCGGGC AGCCACAGTC ACTAAGAAGA CTTACGAGAT CTGGCACCGT 
CGCCATGATT ACTGGCTGCT GGCTGGCATT ATAAACCACG GCTATGCCCG GTGGCAGGAC 
ATCCAGAATG ACCCACGGTA TGCCATCCTC AATGAGCCTT TCAAGGGTGA AATGAATCGT 
GGCAATTTTC TAGAGATCAA GAATAAATTT TTAGCTCGAA GATTCAAGCT CTTAGAACAA 
GCCCTGGTGA TCGAGGAGCA GCTTCGCAGG GCAGCTTACC TGAACATGTC CGAGGACCCC 
TCTCACCCGT CTATGGCCCT TAACACCCGC TTCGCTGAGG TGGAGTGTTT GGCAGAAAGT 
CACCAGCACC TGTCCAAGGA GTCGATGGCA GGGAATAAGC CGGCCAATGC AGTCCTACAC 
AAAGGTCTCC TGAAACAGCT GGAGGAACTG TTGAGTGACA TGAAAGCTGA TGTGACGCGG 
CTCCCAGCCA CCATTGCCCG CATCCCCCCA GTCGCTGTGA GGTTACAGAT GTCAGAGCGG 
AACATTCTCA GCCGCCTGGC AAACAGGGCG CCAGAACCTC CCCCACAGCA GGTAGCCCAG 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505350.1
CDS	355..6123
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGLLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006505287.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505287.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AAGAGGAGGT AGAACGGGAA 
ATCATAAAAC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTAGCCCGA AATCTGGGCA AAGGAAAAAG AATCCGTAAA 
CAGGTCAACT ACAATGATGG CTCACAGGAG GACCGAGATT GGCAGGACGA CCAGTCCGAC 
AACCAGTCCG ATTACTCAGT GGCCTCAGAG GAAGGTGATG AAGACTTTGA CGAACGGTCA 
GAAGCTCCCC GCAGGCCCAG TCGCAAGGGC CTGCGGAATG ATAAAGATAA GCCATTACCT 
CCTCTGTTGG CCCGTGTTGG TGGGAATATT GAAGTACTTG GTTTTAATGC TCGTCAGCGA 
AAAGCATTTC TTAATGCAAT TATGCGATAT GGCATGCCAC CTCAGGATGC TTTTACCACT 
CAGTGGCTTG TGAGAGATCT TCGAGGCAAG TCAGAGAAAG AATTTAAGGC TTATGTGTCA 
CTCTTCATGC GACATTTGTG TGAGCCTGGG GCAGATGGGG CTGAGACCTT TGCTGATGGC 
GTCCCACGAG AAGGCCTGTC TCGTCAACAT GTTCTCACTA GGATCGGTGT CATGTCTCTG 
ATTCGAAAGA AGGTTCAGGA GTTTGAACAT GTTAATGGAC GCTGGAGCAT GCCCGAACTG 
GCTGAAGTAG AGGAAAACAA AAAAATGTCT CAGCCAGGTT CACCTTCTCC AAAGACTCCT 
ACACCTTCCA CACCAGGGGA CACACAACCC AATACTCCTG CGCCTGTGCC ACCTGCTGAG 
GATGGGATAA AAATAGAGGA AAATAGCCTT AAAGAAGAAG AGAGCACAGA AGGAGAGAAG 
GAAGTTAAAT CTACAGCCCC TGAGGCGACT GTGGAGTGTG CACAGCCCCC TGCCCCTGCC 
CCTGCCACTG CCCCTGCCAC TGCCACTGCC CCTGAGGATG ACAAGGCTCC TGCTGAGCCT 
CCTGAGGGGG AGGAAAAAGT GGAAAAGGCA GAGGTGAAGG AGAGAACAGA GGAGCCTATG 
GAGACAGAGG CCAAAGGTAC TACTGAGGTG GAGAAAGTGG AGGAGAAGTC TGCGGTAGAC 
CTGACCCCGA TCGTGGTGGA AGACAAAGAA GAGAAGAAGG AGGAGGAGGA GAAAAAGGAC 
GTGATGCTTC AGAATGGAGA AACTCCCAAG GATCTTAGTG ATGAGAAGCA GAAGAAAAAC 
AGCAAACAGC GTTTCATGTT CAACATTGCA GATGGTGGTT TTACTGAGTT GCACTCACTG 
TGGCAGAATG AAGAGCGGGC AGCCACAGTC ACTAAGAAGA CTTACGAGAT CTGGCACCGT 
CGCCATGATT ACTGGCTGCT GGCTGGCATT ATAAACCACG GCTATGCCCG GTGGCAGGAC 
ATCCAGAATG ACCCACGGTA TGCCATCCTC AATGAGCCTT TCAAGGGTGA AATGAATCGT 
GGCAATTTTC TAGAGATCAA GAATAAATTT TTAGCTCGAA GATTCAAGCT CTTAGAACAA 
GCCCTGGTGA TCGAGGAGCA GCTTCGCAGG GCAGCTTACC TGAACATGTC CGAGGACCCC 
TCTCACCCGT CTATGGCCCT TAACACCCGC TTCGCTGAGG TGGAGTGTTT GGCAGAAAGT 
CACCAGCACC TGTCCAAGGA GTCGATGGCA GGGAATAAGC CGGCCAATGC AGTCCTACAC 
AAAGGTCTCC TGAAACAGCT GGAGGAACTG TTGAGTGACA TGAAAGCTGA TGTGACGCGG 
CTCCCAGCCA CCATTGCCCG CATCCCCCCA GTCGCTGTGA GGTTACAGAT GTCAGAGCGG 
AACATTCTCA GCCGCCTGGC AAACAGGGCG CCAGAACCTC CCCCACAGCA GGTAGCCCAG 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_017321292.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176781.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X3, mRNA.

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CloneID	OMu81595
Clone ID Related Accession (Same CDS sequence)	XM_017321292.1
Accession Version	XM_017321292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AAGAGGAGGT AGAACGGGAA 
ATCATAAAAC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTAGCCCGA AATCTGGGCA AAGGAAAAAG AATCCGTAAA 
CAGGTCAACT ACAATGATGG CTCACAGGAG GACCGAGGTG TGTGTGGCCG GCCCCGCCCC 
CCACCCACGG GCCGTTCCAC TAGAGCAGTG GGCCCCGCTC ATCTGCCCTC TCTCCCTCCA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTACT CAGTGGCCTC AGAGGAAGGT 
GATGAAGACT TTGACGAACG GTCAGAAGCT CCCCGCAGGC CCAGTCGCAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT ACCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTA 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCA TTTCTTAATG CAATTATGCG ATATGGCATG 
CCACCTCAGG ATGCTTTTAC CACTCAGTGG CTTGTGAGAG ATCTTCGAGG CAAGTCAGAG 
AAAGAATTTA AGGCTTATGT GTCACTCTTC ATGCGACATT TGTGTGAGCC TGGGGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGCGTCCCA CGAGAAGGCC TGTCTCGTCA ACATGTTCTC 
ACTAGGATCG GTGTCATGTC TCTGATTCGA AAGAAGGTTC AGGAGTTTGA ACATGTTAAT 
GGACGCTGGA GCATGCCCGA ACTGGCTGAA GTAGAGGAAA ACAAAAAAAT GTCTCAGCCA 
GGTTCACCTT CTCCAAAGAC TCCTACACCT TCCACACCAG GGGACACACA ACCCAATACT 
CCTGCGCCTG TGCCACCTGC TGAGGATGGG ATAAAAATAG AGGAAAATAG CCTTAAAGAA 
GAAGAGAGCA CAGAAGGAGA GAAGGAAGTT AAATCTACAG CCCCTGAGGC GACTGTGGAG 
TGTGCACAGC CCCCTGCCCC TGCCCCTGCC ACTGCCCCTG CCACTGCCAC TGCCCCTGAG 
GATGACAAGG CTCCTGCTGA GCCTCCTGAG GGGGAGGAAA AAGTGGAAAA GGCAGAGGTG 
AAGGAGAGAA CAGAGGAGCC TATGGAGACA GAGGCCAAAG GTACTACTGA GGTGGAGAAA 
GTGGAGGAGA AGTCTGCGGT AGACCTGACC CCGATCGTGG TGGAAGACAA AGAAGAGAAG 
AAGGAGGAGG AGGAGAAAAA GGACGTGATG CTTCAGAATG GAGAAACTCC CAAGGATCTT 
AGTGATGAGA AGCAGAAGAA AAACAGCAAA CAGCGTTTCA TGTTCAACAT TGCAGATGGT 
GGTTTTACTG AGTTGCACTC ACTGTGGCAG AATGAAGAGC GGGCAGCCAC AGTCACTAAG 
AAGACTTACG AGATCTGGCA CCGTCGCCAT GATTACTGGC TGCTGGCTGG CATTATAAAC 
CACGGCTATG CCCGGTGGCA GGACATCCAG AATGACCCAC GGTATGCCAT CCTCAATGAG 
CCTTTCAAGG GTGAAATGAA TCGTGGCAAT TTTCTAGAGA TCAAGAATAA ATTTTTAGCT 
CGAAGATTCA AGCTCTTAGA ACAAGCCCTG GTGATCGAGG AGCAGCTTCG CAGGGCAGCT 
TACCTGAACA TGTCCGAGGA CCCCTCTCAC CCGTCTATGG CCCTTAACAC CCGCTTCGCT 
GAGGTGGAGT GTTTGGCAGA AAGTCACCAG CACCTGTCCA AGGAGTCGAT GGCAGGGAAT 
AAGCCGGCCA ATGCAGTCCT ACACAAAGTC CTGAAACAGC TGGAGGAACT GTTGAGTGAC 
ATGAAAGCTG ATGTGACGCG GCTCCCAGCC ACCATTGCCC GCATCCCCCC AGTCGCTGTG 
AGGTTACAGA TGTCAGAGCG GAACATTCTC AGCCGCCTGG CAAACAGGGC GCCAGAACCT 
CCCCCACAGC AGGTAGCCCA GCAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176781.1
CDS	355..6204
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPTGRSTRAVGPAHLPSLPPDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017321292.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321292.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AAGAGGAGGT AGAACGGGAA 
ATCATAAAAC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTAGCCCGA AATCTGGGCA AAGGAAAAAG AATCCGTAAA 
CAGGTCAACT ACAATGATGG CTCACAGGAG GACCGAGGTG TGTGTGGCCG GCCCCGCCCC 
CCACCCACGG GCCGTTCCAC TAGAGCAGTG GGCCCCGCTC ATCTGCCCTC TCTCCCTCCA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTACT CAGTGGCCTC AGAGGAAGGT 
GATGAAGACT TTGACGAACG GTCAGAAGCT CCCCGCAGGC CCAGTCGCAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT ACCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTA 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCA TTTCTTAATG CAATTATGCG ATATGGCATG 
CCACCTCAGG ATGCTTTTAC CACTCAGTGG CTTGTGAGAG ATCTTCGAGG CAAGTCAGAG 
AAAGAATTTA AGGCTTATGT GTCACTCTTC ATGCGACATT TGTGTGAGCC TGGGGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGCGTCCCA CGAGAAGGCC TGTCTCGTCA ACATGTTCTC 
ACTAGGATCG GTGTCATGTC TCTGATTCGA AAGAAGGTTC AGGAGTTTGA ACATGTTAAT 
GGACGCTGGA GCATGCCCGA ACTGGCTGAA GTAGAGGAAA ACAAAAAAAT GTCTCAGCCA 
GGTTCACCTT CTCCAAAGAC TCCTACACCT TCCACACCAG GGGACACACA ACCCAATACT 
CCTGCGCCTG TGCCACCTGC TGAGGATGGG ATAAAAATAG AGGAAAATAG CCTTAAAGAA 
GAAGAGAGCA CAGAAGGAGA GAAGGAAGTT AAATCTACAG CCCCTGAGGC GACTGTGGAG 
TGTGCACAGC CCCCTGCCCC TGCCCCTGCC ACTGCCCCTG CCACTGCCAC TGCCCCTGAG 
GATGACAAGG CTCCTGCTGA GCCTCCTGAG GGGGAGGAAA AAGTGGAAAA GGCAGAGGTG 
AAGGAGAGAA CAGAGGAGCC TATGGAGACA GAGGCCAAAG GTACTACTGA GGTGGAGAAA 
GTGGAGGAGA AGTCTGCGGT AGACCTGACC CCGATCGTGG TGGAAGACAA AGAAGAGAAG 
AAGGAGGAGG AGGAGAAAAA GGACGTGATG CTTCAGAATG GAGAAACTCC CAAGGATCTT 
AGTGATGAGA AGCAGAAGAA AAACAGCAAA CAGCGTTTCA TGTTCAACAT TGCAGATGGT 
GGTTTTACTG AGTTGCACTC ACTGTGGCAG AATGAAGAGC GGGCAGCCAC AGTCACTAAG 
AAGACTTACG AGATCTGGCA CCGTCGCCAT GATTACTGGC TGCTGGCTGG CATTATAAAC 
CACGGCTATG CCCGGTGGCA GGACATCCAG AATGACCCAC GGTATGCCAT CCTCAATGAG 
CCTTTCAAGG GTGAAATGAA TCGTGGCAAT TTTCTAGAGA TCAAGAATAA ATTTTTAGCT 
CGAAGATTCA AGCTCTTAGA ACAAGCCCTG GTGATCGAGG AGCAGCTTCG CAGGGCAGCT 
TACCTGAACA TGTCCGAGGA CCCCTCTCAC CCGTCTATGG CCCTTAACAC CCGCTTCGCT 
GAGGTGGAGT GTTTGGCAGA AAGTCACCAG CACCTGTCCA AGGAGTCGAT GGCAGGGAAT 
AAGCCGGCCA ATGCAGTCCT ACACAAAGTC CTGAAACAGC TGGAGGAACT GTTGAGTGAC 
ATGAAAGCTG ATGTGACGCG GCTCCCAGCC ACCATTGCCC GCATCCCCCC AGTCGCTGTG 
AGGTTACAGA TGTCAGAGCG GAACATTCTC AGCCGCCTGG CAAACAGGGC GCCAGAACCT 
CCCCCACAGC AGGTAGCCCA GCAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_017321294.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176783.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X5, mRNA.

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CloneID	OMu81596
Clone ID Related Accession (Same CDS sequence)	XM_017321294.1
Accession Version	XM_017321294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AAGAGGAGGT AGAACGGGAA 
ATCATAAAAC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTAGCCCGA AATCTGGGCA AAGGAAAAAG AATCCGTAAA 
CAGGTCAACT ACAATGATGG CTCACAGGAG GACCGAGATT GGCAGGACGA CCAGTCCGAC 
AACCAGTCCG ATTACTCAGT GGCCTCAGAG GAAGGTGATG AAGACTTTGA CGAACGGTCA 
GAAGCTCCCC GCAGGCCCAG TCGCAAGGGC CTGCGGAATG ATAAAGATAA GCCATTACCT 
CCTCTGTTGG CCCGTGTTGG TGGGAATATT GAAGTACTTG GTTTTAATGC TCGTCAGCGA 
AAAGCATTTC TTAATGCAAT TATGCGATAT GGCATGCCAC CTCAGGATGC TTTTACCACT 
CAGTGGCTTG TGAGAGATCT TCGAGGCAAG TCAGAGAAAG AATTTAAGGC TTATGTGTCA 
CTCTTCATGC GACATTTGTG TGAGCCTGGG GCAGATGGGG CTGAGACCTT TGCTGATGGC 
GTCCCACGAG AAGGCCTGTC TCGTCAACAT GTTCTCACTA GGATCGGTGT CATGTCTCTG 
ATTCGAAAGA AGGTTCAGGA GTTTGAACAT GTTAATGGAC GCTGGAGCAT GCCCGAACTG 
GCTGAAGTAG AGGAAAACAA AAAAATGTCT CAGCCAGGTT CACCTTCTCC AAAGACTCCT 
ACACCTTCCA CACCAGGGGA CACACAACCC AATACTCCTG CGCCTGTGCC ACCTGCTGAG 
GATGGGATAA AAATAGAGGA AAATAGCCTT AAAGAAGAAG AGAGCACAGA AGGAGAGAAG 
GAAGTTAAAT CTACAGCCCC TGAGGCGACT GTGGAGTGTG CACAGCCCCC TGCCCCTGCC 
CCTGCCACTG CCCCTGCCAC TGCCACTGCC CCTGAGGATG ACAAGGCTCC TGCTGAGCCT 
CCTGAGGGGG AGGAAAAAGT GGAAAAGGCA GAGGTGAAGG AGAGAACAGA GGAGCCTATG 
GAGACAGAGG CCAAAGGTAC TACTGAGGTG GAGAAAGTGG AGGAGAAGTC TGCGGTAGAC 
CTGACCCCGA TCGTGGTGGA AGACAAAGAA GAGAAGAAGG AGGAGGAGGA GAAAAAGGAC 
GTGATGCTTC AGAATGGAGA AACTCCCAAG GATCTTAGTG ATGAGAAGCA GAAGAAAAAC 
AGCAAACAGC GTTTCATGTT CAACATTGCA GATGGTGGTT TTACTGAGTT GCACTCACTG 
TGGCAGAATG AAGAGCGGGC AGCCACAGTC ACTAAGAAGA CTTACGAGAT CTGGCACCGT 
CGCCATGATT ACTGGCTGCT GGCTGGCATT ATAAACCACG GCTATGCCCG GTGGCAGGAC 
ATCCAGAATG ACCCACGGTA TGCCATCCTC AATGAGCCTT TCAAGGGTGA AATGAATCGT 
GGCAATTTTC TAGAGATCAA GAATAAATTT TTAGCTCGAA GATTCAAGCT CTTAGAACAA 
GCCCTGGTGA TCGAGGAGCA GCTTCGCAGG GCAGCTTACC TGAACATGTC CGAGGACCCC 
TCTCACCCGT CTATGGCCCT TAACACCCGC TTCGCTGAGG TGGAGTGTTT GGCAGAAAGT 
CACCAGCACC TGTCCAAGGA GTCGATGGCA GGGAATAAGC CGGCCAATGC AGTCCTACAC 
AAAGTCCTGA AACAGCTGGA GGAACTGTTG AGTGACATGA AAGCTGATGT GACGCGGCTC 
CCAGCCACCA TTGCCCGCAT CCCCCCAGTC GCTGTGAGGT TACAGATGTC AGAGCGGAAC 
ATTCTCAGCC GCCTGGCAAA CAGGGCGCCA GAACCTCCCC CACAGCAGGT AGCCCAGCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176783.1
CDS	355..6120
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKERLLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017321294.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321294.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTTTACT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA AAAGAAGCTC 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA AGAGGAGGAC 
GAGGATGATG ACTCGAAGGA ACCTAAGTCA TCAGCCCAGC TTTTAGAAGA CTGGGGTATG 
GAAGACATCG ACCATGTGTT CTCAGAGGAG GATTATCGGA CCCTCACCAA CTACAAGGCC 
TTCAGCCAAT TTGTTAGACC CCTGATTGCT GCTAAAAACC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TCTTGGGTGC AAAGTGGAGG GAATTCAGTA CCAACAACCC CTTCAAGGGC 
AGCTCCGGAG CATCAGTGGC AGCTGCGGCG GCGGCGGCTG TGGCTGTGGT TGAGAGCATG 
GTGACAGCTA CTGAAGTCGC CCCTCCCCCT CCCCCTGTGG AGGTGCCGAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CGAAAGGCAG CCCGCGAGTG 
CCTGACGCCA AGAAGCCCAA ACCCAAGAAA GTAGCTCCTC TGAAGATCAA GCTGGGAGGG 
TTCGGCTCCA AGCGGAAGAG ATCCTCGAGT GAGGACGACG ATTTAGATGT AGAGTCTGAC 
TTCGACGATG CCAGTATCAA CAGCTATTCT GTTTCGGATG GTTCCACCAG CCGCAGTAGT 
CGGAGCCGTA AGAAACTCCG GACCGCTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AAGAGGAGGT AGAACGGGAA 
ATCATAAAAC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTAGCCCGA AATCTGGGCA AAGGAAAAAG AATCCGTAAA 
CAGGTCAACT ACAATGATGG CTCACAGGAG GACCGAGATT GGCAGGACGA CCAGTCCGAC 
AACCAGTCCG ATTACTCAGT GGCCTCAGAG GAAGGTGATG AAGACTTTGA CGAACGGTCA 
GAAGCTCCCC GCAGGCCCAG TCGCAAGGGC CTGCGGAATG ATAAAGATAA GCCATTACCT 
CCTCTGTTGG CCCGTGTTGG TGGGAATATT GAAGTACTTG GTTTTAATGC TCGTCAGCGA 
AAAGCATTTC TTAATGCAAT TATGCGATAT GGCATGCCAC CTCAGGATGC TTTTACCACT 
CAGTGGCTTG TGAGAGATCT TCGAGGCAAG TCAGAGAAAG AATTTAAGGC TTATGTGTCA 
CTCTTCATGC GACATTTGTG TGAGCCTGGG GCAGATGGGG CTGAGACCTT TGCTGATGGC 
GTCCCACGAG AAGGCCTGTC TCGTCAACAT GTTCTCACTA GGATCGGTGT CATGTCTCTG 
ATTCGAAAGA AGGTTCAGGA GTTTGAACAT GTTAATGGAC GCTGGAGCAT GCCCGAACTG 
GCTGAAGTAG AGGAAAACAA AAAAATGTCT CAGCCAGGTT CACCTTCTCC AAAGACTCCT 
ACACCTTCCA CACCAGGGGA CACACAACCC AATACTCCTG CGCCTGTGCC ACCTGCTGAG 
GATGGGATAA AAATAGAGGA AAATAGCCTT AAAGAAGAAG AGAGCACAGA AGGAGAGAAG 
GAAGTTAAAT CTACAGCCCC TGAGGCGACT GTGGAGTGTG CACAGCCCCC TGCCCCTGCC 
CCTGCCACTG CCCCTGCCAC TGCCACTGCC CCTGAGGATG ACAAGGCTCC TGCTGAGCCT 
CCTGAGGGGG AGGAAAAAGT GGAAAAGGCA GAGGTGAAGG AGAGAACAGA GGAGCCTATG 
GAGACAGAGG CCAAAGGTAC TACTGAGGTG GAGAAAGTGG AGGAGAAGTC TGCGGTAGAC 
CTGACCCCGA TCGTGGTGGA AGACAAAGAA GAGAAGAAGG AGGAGGAGGA GAAAAAGGAC 
GTGATGCTTC AGAATGGAGA AACTCCCAAG GATCTTAGTG ATGAGAAGCA GAAGAAAAAC 
AGCAAACAGC GTTTCATGTT CAACATTGCA GATGGTGGTT TTACTGAGTT GCACTCACTG 
TGGCAGAATG AAGAGCGGGC AGCCACAGTC ACTAAGAAGA CTTACGAGAT CTGGCACCGT 
CGCCATGATT ACTGGCTGCT GGCTGGCATT ATAAACCACG GCTATGCCCG GTGGCAGGAC 
ATCCAGAATG ACCCACGGTA TGCCATCCTC AATGAGCCTT TCAAGGGTGA AATGAATCGT 
GGCAATTTTC TAGAGATCAA GAATAAATTT TTAGCTCGAA GATTCAAGCT CTTAGAACAA 
GCCCTGGTGA TCGAGGAGCA GCTTCGCAGG GCAGCTTACC TGAACATGTC CGAGGACCCC 
TCTCACCCGT CTATGGCCCT TAACACCCGC TTCGCTGAGG TGGAGTGTTT GGCAGAAAGT 
CACCAGCACC TGTCCAAGGA GTCGATGGCA GGGAATAAGC CGGCCAATGC AGTCCTACAC 
AAAGTCCTGA AACAGCTGGA GGAACTGTTG AGTGACATGA AAGCTGATGT GACGCGGCTC 
CCAGCCACCA TTGCCCGCAT CCCCCCAGTC GCTGTGAGGT TACAGATGTC AGAGCGGAAC 
ATTCTCAGCC GCCTGGCAAA CAGGGCGCCA GAACCTCCCC CACAGCAGGT AGCCCAGCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_017321295.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176784.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X11, mRNA.

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CloneID	OMu81597
Clone ID Related Accession (Same CDS sequence)	XM_017321295.1 , XM_017321295.2
Accession Version	XM_017321295.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGCG AGATCATCCT GTGTGATACC 
TGTCCCCGAG CCTACCATAT GGTGTGCCTG GACCCAGACA TGGAGAAGGC CCCGGAGGGC 
AAGTGGAGCT GTCCGCACTG TGAGAAGGAG GGGATCCAGT GGGAAGCTAA GGAGGACAAT 
TCTGAGGGTG AGGAGATTCT GGAAGAAGTC GGGGGGGACC CAGAAGAGGA GGATGACCAT 
CACATGGAAT TCTGTCGCGT CTGCAAGGAC GGCGGGGAGC TCCTGTGCTG TGACACATGC 
CCTTCTTCCT ACCACATCCA CTGCCTGAAC CCCCCGCTGC CAGAGATCCC AAACGGCGAA 
TGGCTCTGTC CCCGCTGTAC TTGTCCAGCT CTTAAGGGCA AAGTGCAGAA GATCCTAATT 
TGGAAGTGGG GCCAGCCACC GTCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGACCCT 
AATACCCCGT CGCCCAAGCC CTTGGAGGGG CGGCCAGAGA GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTGTCAGAAC TGCAGTTGGA GCTGCACTGT 
CAGGTGATGT TCAGAAACTA CCAGCGGAAG AATGATATGG ACGAGCCGCC TTCTGGGGAC 
TTTGGTGGAG ATGAAGAGAA GAGCCGGAAG CGAAAGAACA AGGACCCTAA GTTTGCAGAG 
ATGGAAGAGC GCTTCTACCG CTATGGAATA AAACCGGAGT GGATGATGAT CCACCGCATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTTCACTATT TAATCAAGTG GCGAGACCTG 
CCCTATGACC AGGCGTCCTG GGAGAGCGAG GACGTGGAGA TTCAGGACTA CGACCTGTTC 
AAGCAGAGCT ACTGGAACCA CAGGGAGTTA ATGAGGGGTG AAGAAGGACG ACCCGGCAAG 
AAGCTTAAGA AGGTGAAGCT ACGGAAACTG GAAAGGCCTC CGGAGACCCC AACTGTTGAT 
CCCACCGTGA AGTATGAGCG ACAGCCGGAG TATCTGGATG CGACGGGTGG AACCCTGCAT 
CCCTACCAAA TGGAAGGCTT AAACTGGCTC CGCTTCTCCT GGGCTCAGGG AACCGATACT 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTGCAGA CAGCAGTCTT CCTCTACTCC 
CTCTATAAGG AGGGTCACTC CAAAGGCCCC TTCTTAGTGA GCGCTCCTCT GTCCACCATC 
ATCAACTGGG AGAGAGAGTT TGAGATGTGG GCTCCTGATA TGTATGTGGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGG GAAAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCGAGGATG AAGAAAGAAG CATCGGTGAA ATTCCATGTT 
CTGCTGACAT CCTATGAGTT GATCACCATT GATATGGCCA TCTTGGGTTC TATTGACTGG 
GCCTGCCTCA TTGTGGATGA AGCCCATCGG CTGAAGAACA ACCAGTCTAA GTTCTTTCGA 
GTTTTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTAA CTGGAACTCC GTTACAGAAC 
AATCTAGAGG AACTGTTTCA TCTGCTCAAC TTTCTCACCC CTGAGAGGTT CCACAACTTA 
GAAGGCTTCT TGGAGGAGTT TGCAGACATT GCCAAAGAGG ACCAGATTAA AAAACTCCAC 
GACATGCTGG GGCCTCATAT GTTGCGGCGG CTCAAAGCTG ACGTCTTCAA GAATATGCCA 
TCCAAGACAG AGCTGATTGT GCGCGTGGAG TTGAGTCCTA TGCAGAAGAA GTACTACAAG 
TATATTCTCA CGCGGAATTT CGAAGCACTT AATGCTCGAG GTGGTGGCAA CCAGGTTTCT 
CTGCTGAACG TGGTGATGGA TCTTAAGAAA TGCTGCAACC ACCCTTATCT CTTCCCTGTG 
GCAGCAATGG AAGCCCCTAA GATGCCTAAT GGTATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AGTTGTTGCT GCTGCAGAAG ATGCTTAAGA ACCTGAAAGA GGGAGGGCAT 
CGTGTGCTCA TCTTCTCCCA GATGACCAAG ATGTTGGACT TGCTAGAGGA TTTTTTAGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGTGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGATC GCTTCAATGC ACCGGGTGCT CAACAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGGATCAA TCTGGCCACT GCGGACACAG TTATTATATA TGACTCTGAC 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCCC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TCTACCGGTT TGTGACCCGT GCATCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AGATGATGCT GACACATTTA GTGGTACGGC CTGGCCTGGG CTCCAAGACA 
GGCTCAATGT CTAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAGCTATTC 
AAGGATGAAG CCACGGATGG AGGAGGAGAC AACAAGGAGG GAGAAGACAG CAGTGTTATC 
CACTATGACG ATAAGGCCAT TGAACGACTG CTGGATCGAA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCTCA GTATGTGGTA 
CGGGAAGAAG AGATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATAAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCACAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCCTCAG AGGAAGGTGA TGAAGACTTT GACGAACGGT CAGAAGCTCC CCGCAGGCCC 
AGTCGCAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTAC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCATT TCTTAATGCA 
ATTATGCGAT ATGGCATGCC ACCTCAGGAT GCTTTTACCA CTCAGTGGCT TGTGAGAGAT 
CTTCGAGGCA AGTCAGAGAA AGAATTTAAG GCTTATGTGT CACTCTTCAT GCGACATTTG 
TGTGAGCCTG GGGCAGATGG GGCTGAGACC TTTGCTGATG GCGTCCCACG AGAAGGCCTG 
TCTCGTCAAC ATGTTCTCAC TAGGATCGGT GTCATGTCTC TGATTCGAAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG ACGCTGGAGC ATGCCCGAAC TGGCTGAAGT AGAGGAAAAC 
AAAAAAATGT CTCAGCCAGG TTCACCTTCT CCAAAGACTC CTACACCTTC CACACCAGGG 
GACACACAAC CCAATACTCC TGCGCCTGTG CCACCTGCTG AGGATGGGAT AAAAATAGAG 
GAAAATAGCC TTAAAGAAGA AGAGAGCACA GAAGGAGAGA AGGAAGTTAA ATCTACAGCC 
CCTGAGGCGA CTGTGGAGTG TGCACAGCCC CCTGCCCCTG CCCCTGCCAC TGCCCCTGCC 
ACTGCCACTG CCCCTGAGGA TGACAAGGCT CCTGCTGAGC CTCCTGAGGG GGAGGAAAAA 
GTGGAAAAGG CAGAGGTGAA GGAGAGAACA GAGGAGCCTA TGGAGACAGA GGCCAAAGGT 
ACTACTGAGG TGGAGAAAGT GGAGGAGAAG TCTGCGGTAG ACCTGACCCC GATCGTGGTG 
GAAGACAAAG AAGAGAAGAA GGAGGAGGAG GAGAAAAAGG ACGTGATGCT TCAGAATGGA 
GAAACTCCCA AGGATCTTAG TGATGAGAAG CAGAAGAAAA ACAGCAAACA GCGTTTCATG 
TTCAACATTG CAGATGGTGG TTTTACTGAG TTGCACTCAC TGTGGCAGAA TGAAGAGCGG 
GCAGCCACAG TCACTAAGAA GACTTACGAG ATCTGGCACC GTCGCCATGA TTACTGGCTG 
CTGGCTGGCA TTATAAACCA CGGCTATGCC CGGTGGCAGG ACATCCAGAA TGACCCACGG 
TATGCCATCC TCAATGAGCC TTTCAAGGGT GAAATGAATC GTGGCAATTT TCTAGAGATC 
AAGAATAAAT TTTTAGCTCG AAGATTCAAG CTCTTAGAAC AAGCCCTGGT GATCGAGGAG 
CAGCTTCGCA GGGCAGCTTA CCTGAACATG TCCGAGGACC CCTCTCACCC GTCTATGGCC 
CTTAACACCC GCTTCGCTGA GGTGGAGTGT TTGGCAGAAA GTCACCAGCA CCTGTCCAAG 
GAGTCGATGG CAGGGAATAA GCCGGCCAAT GCAGTCCTAC ACAAAGGTCT CCTGAAACAG 
CTGGAGGAAC TGTTGAGTGA CATGAAAGCT GATGTGACGC GGCTCCCAGC CACCATTGCC 
CGCATCCCCC CAGTCGCTGT GAGGTTACAG ATGTCAGAGC GGAACATTCT CAGCCGCCTG 
GCAAACAGGG CGCCAGAACC TCCCCCACAG CAGGTAGCCC AGCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176784.1
CDS	355..6105
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGLLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017321295.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321295.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGCG AGATCATCCT GTGTGATACC 
TGTCCCCGAG CCTACCATAT GGTGTGCCTG GACCCAGACA TGGAGAAGGC CCCGGAGGGC 
AAGTGGAGCT GTCCGCACTG TGAGAAGGAG GGGATCCAGT GGGAAGCTAA GGAGGACAAT 
TCTGAGGGTG AGGAGATTCT GGAAGAAGTC GGGGGGGACC CAGAAGAGGA GGATGACCAT 
CACATGGAAT TCTGTCGCGT CTGCAAGGAC GGCGGGGAGC TCCTGTGCTG TGACACATGC 
CCTTCTTCCT ACCACATCCA CTGCCTGAAC CCCCCGCTGC CAGAGATCCC AAACGGCGAA 
TGGCTCTGTC CCCGCTGTAC TTGTCCAGCT CTTAAGGGCA AAGTGCAGAA GATCCTAATT 
TGGAAGTGGG GCCAGCCACC GTCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGACCCT 
AATACCCCGT CGCCCAAGCC CTTGGAGGGG CGGCCAGAGA GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTGTCAGAAC TGCAGTTGGA GCTGCACTGT 
CAGGTGATGT TCAGAAACTA CCAGCGGAAG AATGATATGG ACGAGCCGCC TTCTGGGGAC 
TTTGGTGGAG ATGAAGAGAA GAGCCGGAAG CGAAAGAACA AGGACCCTAA GTTTGCAGAG 
ATGGAAGAGC GCTTCTACCG CTATGGAATA AAACCGGAGT GGATGATGAT CCACCGCATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTTCACTATT TAATCAAGTG GCGAGACCTG 
CCCTATGACC AGGCGTCCTG GGAGAGCGAG GACGTGGAGA TTCAGGACTA CGACCTGTTC 
AAGCAGAGCT ACTGGAACCA CAGGGAGTTA ATGAGGGGTG AAGAAGGACG ACCCGGCAAG 
AAGCTTAAGA AGGTGAAGCT ACGGAAACTG GAAAGGCCTC CGGAGACCCC AACTGTTGAT 
CCCACCGTGA AGTATGAGCG ACAGCCGGAG TATCTGGATG CGACGGGTGG AACCCTGCAT 
CCCTACCAAA TGGAAGGCTT AAACTGGCTC CGCTTCTCCT GGGCTCAGGG AACCGATACT 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTGCAGA CAGCAGTCTT CCTCTACTCC 
CTCTATAAGG AGGGTCACTC CAAAGGCCCC TTCTTAGTGA GCGCTCCTCT GTCCACCATC 
ATCAACTGGG AGAGAGAGTT TGAGATGTGG GCTCCTGATA TGTATGTGGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGG GAAAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCGAGGATG AAGAAAGAAG CATCGGTGAA ATTCCATGTT 
CTGCTGACAT CCTATGAGTT GATCACCATT GATATGGCCA TCTTGGGTTC TATTGACTGG 
GCCTGCCTCA TTGTGGATGA AGCCCATCGG CTGAAGAACA ACCAGTCTAA GTTCTTTCGA 
GTTTTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTAA CTGGAACTCC GTTACAGAAC 
AATCTAGAGG AACTGTTTCA TCTGCTCAAC TTTCTCACCC CTGAGAGGTT CCACAACTTA 
GAAGGCTTCT TGGAGGAGTT TGCAGACATT GCCAAAGAGG ACCAGATTAA AAAACTCCAC 
GACATGCTGG GGCCTCATAT GTTGCGGCGG CTCAAAGCTG ACGTCTTCAA GAATATGCCA 
TCCAAGACAG AGCTGATTGT GCGCGTGGAG TTGAGTCCTA TGCAGAAGAA GTACTACAAG 
TATATTCTCA CGCGGAATTT CGAAGCACTT AATGCTCGAG GTGGTGGCAA CCAGGTTTCT 
CTGCTGAACG TGGTGATGGA TCTTAAGAAA TGCTGCAACC ACCCTTATCT CTTCCCTGTG 
GCAGCAATGG AAGCCCCTAA GATGCCTAAT GGTATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AGTTGTTGCT GCTGCAGAAG ATGCTTAAGA ACCTGAAAGA GGGAGGGCAT 
CGTGTGCTCA TCTTCTCCCA GATGACCAAG ATGTTGGACT TGCTAGAGGA TTTTTTAGAA 
CATGAAGGTT ATAAATATGA ACGTATTGAT GGTGGAATCA CTGGGAACAT GCGTCAGGAG 
GCCATTGATC GCTTCAATGC ACCGGGTGCT CAACAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGGATCAA TCTGGCCACT GCGGACACAG TTATTATATA TGACTCTGAC 
TGGAACCCCC ATAATGACAT CCAGGCCTTT AGCAGAGCCC ACCGTATTGG GCAAAATAAG 
AAGGTGATGA TCTACCGGTT TGTGACCCGT GCATCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AGATGATGCT GACACATTTA GTGGTACGGC CTGGCCTGGG CTCCAAGACA 
GGCTCAATGT CTAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAGCTATTC 
AAGGATGAAG CCACGGATGG AGGAGGAGAC AACAAGGAGG GAGAAGACAG CAGTGTTATC 
CACTATGACG ATAAGGCCAT TGAACGACTG CTGGATCGAA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCTCA GTATGTGGTA 
CGGGAAGAAG AGATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATAAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCACAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCCTCAG AGGAAGGTGA TGAAGACTTT GACGAACGGT CAGAAGCTCC CCGCAGGCCC 
AGTCGCAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTAC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCATT TCTTAATGCA 
ATTATGCGAT ATGGCATGCC ACCTCAGGAT GCTTTTACCA CTCAGTGGCT TGTGAGAGAT 
CTTCGAGGCA AGTCAGAGAA AGAATTTAAG GCTTATGTGT CACTCTTCAT GCGACATTTG 
TGTGAGCCTG GGGCAGATGG GGCTGAGACC TTTGCTGATG GCGTCCCACG AGAAGGCCTG 
TCTCGTCAAC ATGTTCTCAC TAGGATCGGT GTCATGTCTC TGATTCGAAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG ACGCTGGAGC ATGCCCGAAC TGGCTGAAGT AGAGGAAAAC 
AAAAAAATGT CTCAGCCAGG TTCACCTTCT CCAAAGACTC CTACACCTTC CACACCAGGG 
GACACACAAC CCAATACTCC TGCGCCTGTG CCACCTGCTG AGGATGGGAT AAAAATAGAG 
GAAAATAGCC TTAAAGAAGA AGAGAGCACA GAAGGAGAGA AGGAAGTTAA ATCTACAGCC 
CCTGAGGCGA CTGTGGAGTG TGCACAGCCC CCTGCCCCTG CCCCTGCCAC TGCCCCTGCC 
ACTGCCACTG CCCCTGAGGA TGACAAGGCT CCTGCTGAGC CTCCTGAGGG GGAGGAAAAA 
GTGGAAAAGG CAGAGGTGAA GGAGAGAACA GAGGAGCCTA TGGAGACAGA GGCCAAAGGT 
ACTACTGAGG TGGAGAAAGT GGAGGAGAAG TCTGCGGTAG ACCTGACCCC GATCGTGGTG 
GAAGACAAAG AAGAGAAGAA GGAGGAGGAG GAGAAAAAGG ACGTGATGCT TCAGAATGGA 
GAAACTCCCA AGGATCTTAG TGATGAGAAG CAGAAGAAAA ACAGCAAACA GCGTTTCATG 
TTCAACATTG CAGATGGTGG TTTTACTGAG TTGCACTCAC TGTGGCAGAA TGAAGAGCGG 
GCAGCCACAG TCACTAAGAA GACTTACGAG ATCTGGCACC GTCGCCATGA TTACTGGCTG 
CTGGCTGGCA TTATAAACCA CGGCTATGCC CGGTGGCAGG ACATCCAGAA TGACCCACGG 
TATGCCATCC TCAATGAGCC TTTCAAGGGT GAAATGAATC GTGGCAATTT TCTAGAGATC 
AAGAATAAAT TTTTAGCTCG AAGATTCAAG CTCTTAGAAC AAGCCCTGGT GATCGAGGAG 
CAGCTTCGCA GGGCAGCTTA CCTGAACATG TCCGAGGACC CCTCTCACCC GTCTATGGCC 
CTTAACACCC GCTTCGCTGA GGTGGAGTGT TTGGCAGAAA GTCACCAGCA CCTGTCCAAG 
GAGTCGATGG CAGGGAATAA GCCGGCCAAT GCAGTCCTAC ACAAAGGTCT CCTGAAACAG 
CTGGAGGAAC TGTTGAGTGA CATGAAAGCT GATGTGACGC GGCTCCCAGC CACCATTGCC 
CGCATCCCCC CAGTCGCTGT GAGGTTACAG ATGTCAGAGC GGAACATTCT CAGCCGCCTG 
GCAAACAGGG CGCCAGAACC TCCCCCACAG CAGGTAGCCC AGCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chd4 ( XM_017321296.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176785.1 Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X12, mRNA.

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CloneID	OMu81598
Clone ID Related Accession (Same CDS sequence)	XM_017321296.1 , XM_017321296.2
Accession Version	XM_017321296.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGG 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTA 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCAT TTCTTAATGC AATTATGCGA TATGGCATGC CACCTCAGGA TGCTTTTACC 
ACTCAGTGGC TTGTGAGAGA TCTTCGAGGC AAGTCAGAGA AAGAATTTAA GGCTTATGTG 
TCACTCTTCA TGCGACATTT GTGTGAGCCT GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGCGTCCCAC GAGAAGGCCT GTCTCGTCAA CATGTTCTCA CTAGGATCGG TGTCATGTCT 
CTGATTCGAA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GACGCTGGAG CATGCCCGAA 
CTGGCTGAAG TAGAGGAAAA CAAAAAAATG TCTCAGCCAG GTTCACCTTC TCCAAAGACT 
CCTACACCTT CCACACCAGG GGACACACAA CCCAATACTC CTGCGCCTGT GCCACCTGCT 
GAGGATGGGA TAAAAATAGA GGAAAATAGC CTTAAAGAAG AAGAGAGCAC AGAAGGAGAG 
AAGGAAGTTA AATCTACAGC CCCTGAGGCG ACTGTGGAGT GTGCACAGCC CCCTGCCCCT 
GCCCCTGCCA CTGCCCCTGC CACTGCCACT GCCCCTGAGG ATGACAAGGC TCCTGCTGAG 
CCTCCTGAGG GGGAGGAAAA AGTGGAAAAG GCAGAGGTGA AGGAGAGAAC AGAGGAGCCT 
ATGGAGACAG AGGCCAAAGG TACTACTGAG GTGGAGAAAG TGGAGGAGAA GTCTGCGGTA 
GACCTGACCC CGATCGTGGT GGAAGACAAA GAAGAGAAGA AGGAGGAGGA GGAGAAAAAG 
GACGTGATGC TTCAGAATGG AGAAACTCCC AAGGATCTTA GTGATGAGAA GCAGAAGAAA 
AACAGCAAAC AGCGTTTCAT GTTCAACATT GCAGATGGTG GTTTTACTGA GTTGCACTCA 
CTGTGGCAGA ATGAAGAGCG GGCAGCCACA GTCACTAAGA AGACTTACGA GATCTGGCAC 
CGTCGCCATG ATTACTGGCT GCTGGCTGGC ATTATAAACC ACGGCTATGC CCGGTGGCAG 
GACATCCAGA ATGACCCACG GTATGCCATC CTCAATGAGC CTTTCAAGGG TGAAATGAAT 
CGTGGCAATT TTCTAGAGAT CAAGAATAAA TTTTTAGCTC GAAGATTCAA GCTCTTAGAA 
CAAGCCCTGG TGATCGAGGA GCAGCTTCGC AGGGCAGCTT ACCTGAACAT GTCCGAGGAC 
CCCTCTCACC CGTCTATGGC CCTTAACACC CGCTTCGCTG AGGTGGAGTG TTTGGCAGAA 
AGTCACCAGC ACCTGTCCAA GGAGTCGATG GCAGGGAATA AGCCGGCCAA TGCAGTCCTA 
CACAAAGTCC TGAAACAGCT GGAGGAACTG TTGAGTGACA TGAAAGCTGA TGTGACGCGG 
CTCCCAGCCA CCATTGCCCG CATCCCCCCA GTCGCTGTGA GGTTACAGAT GTCAGAGCGG 
AACATTCTCA GCCGCCTGGC AAACAGGGCG CCAGAACCTC CCCCACAGCA GGTAGCCCAG 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176785.1
CDS	355..6063
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPDEDLSEAETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDEDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTAKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDPEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESTEGEKEVKSTAPEATVECAQPPAPAPATAPATATAPEDDKAPAEPPEGEEKVEKAEVKERTEEPMETEAKGTTEVEKVEEKSAVDLTPIVVEDKEEKKEEEEKKDVMLQNGETPKDLSDEKQKKNSKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPPPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017321296.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 4 (Chd4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321296.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCAGCGG GCAGTGAGGA GGAGGATATG 
GACGCACTTC TGAACAACAG CCTGCCCCCA CCCCATCCAG AAAATGAGGA AGACCCAGAC 
GAGGATTTAT CCGAAGCAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGAGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAA AAGAAGCTCG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAA GAGGAGGACG AGGATGATGA CTCGAAGGAA 
CCTAAGTCAT CAGCCCAGCT TTTAGAAGAC TGGGGTATGG AAGACATCGA CCATGTGTTC 
TCAGAGGAGG ATTATCGGAC CCTCACCAAC TACAAGGCCT TCAGCCAATT TGTTAGACCC 
CTGATTGCTG CTAAAAACCC CAAGATTGCT GTCTCCAAGA TGATGATGGT CTTGGGTGCA 
AAGTGGAGGG AATTCAGTAC CAACAACCCC TTCAAGGGCA GCTCCGGAGC ATCAGTGGCA 
GCTGCGGCGG CGGCGGCTGT GGCTGTGGTT GAGAGCATGG TGACAGCTAC TGAAGTCGCC 
CCTCCCCCTC CCCCTGTGGA GGTGCCGATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC GAAAGGCAGC CCGCGAGTGC CTGACGCCAA GAAGCCCAAA 
CCCAAGAAAG TAGCTCCTCT GAAGATCAAG CTGGGAGGGT TCGGCTCCAA GCGGAAGAGA 
TCCTCGAGTG AGGACGACGA TTTAGATGTA GAGTCTGACT TCGACGATGC CAGTATCAAC 
AGCTATTCTG TTTCGGATGG TTCCACCAGC CGCAGTAGTC GGAGCCGTAA GAAACTCCGG 
ACCGCTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGC 
GAGATCATCC TGTGTGATAC CTGTCCCCGA GCCTACCATA TGGTGTGCCT GGACCCAGAC 
ATGGAGAAGG CCCCGGAGGG CAAGTGGAGC TGTCCGCACT GTGAGAAGGA GGGGATCCAG 
TGGGAAGCTA AGGAGGACAA TTCTGAGGGT GAGGAGATTC TGGAAGAAGT CGGGGGGGAC 
CCAGAAGAGG AGGATGACCA TCACATGGAA TTCTGTCGCG TCTGCAAGGA CGGCGGGGAG 
CTCCTGTGCT GTGACACATG CCCTTCTTCC TACCACATCC ACTGCCTGAA CCCCCCGCTG 
CCAGAGATCC CAAACGGCGA ATGGCTCTGT CCCCGCTGTA CTTGTCCAGC TCTTAAGGGC 
AAAGTGCAGA AGATCCTAAT TTGGAAGTGG GGCCAGCCAC CGTCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGACCC TAATACCCCG TCGCCCAAGC CCTTGGAGGG GCGGCCAGAG 
AGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTGTCAGAA 
CTGCAGTTGG AGCTGCACTG TCAGGTGATG TTCAGAAACT ACCAGCGGAA GAATGATATG 
GACGAGCCGC CTTCTGGGGA CTTTGGTGGA GATGAAGAGA AGAGCCGGAA GCGAAAGAAC 
AAGGACCCTA AGTTTGCAGA GATGGAAGAG CGCTTCTACC GCTATGGAAT AAAACCGGAG 
TGGATGATGA TCCACCGCAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTTCACTAT 
TTAATCAAGT GGCGAGACCT GCCCTATGAC CAGGCGTCCT GGGAGAGCGA GGACGTGGAG 
ATTCAGGACT ACGACCTGTT CAAGCAGAGC TACTGGAACC ACAGGGAGTT AATGAGGGGT 
GAAGAAGGAC GACCCGGCAA GAAGCTTAAG AAGGTGAAGC TACGGAAACT GGAAAGGCCT 
CCGGAGACCC CAACTGTTGA TCCCACCGTG AAGTATGAGC GACAGCCGGA GTATCTGGAT 
GCGACGGGTG GAACCCTGCA TCCCTACCAA ATGGAAGGCT TAAACTGGCT CCGCTTCTCC 
TGGGCTCAGG GAACCGATAC TATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTGCAG 
ACAGCAGTCT TCCTCTACTC CCTCTATAAG GAGGGTCACT CCAAAGGCCC CTTCTTAGTG 
AGCGCTCCTC TGTCCACCAT CATCAACTGG GAGAGAGAGT TTGAGATGTG GGCTCCTGAT 
ATGTATGTGG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG GGAAAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCGAGGAT GAAGAAAGAA 
GCATCGGTGA AATTCCATGT TCTGCTGACA TCCTATGAGT TGATCACCAT TGATATGGCC 
ATCTTGGGTT CTATTGACTG GGCCTGCCTC ATTGTGGATG AAGCCCATCG GCTGAAGAAC 
AACCAGTCTA AGTTCTTTCG AGTTTTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTA 
ACTGGAACTC CGTTACAGAA CAATCTAGAG GAACTGTTTC ATCTGCTCAA CTTTCTCACC 
CCTGAGAGGT TCCACAACTT AGAAGGCTTC TTGGAGGAGT TTGCAGACAT TGCCAAAGAG 
GACCAGATTA AAAAACTCCA CGACATGCTG GGGCCTCATA TGTTGCGGCG GCTCAAAGCT 
GACGTCTTCA AGAATATGCC ATCCAAGACA GAGCTGATTG TGCGCGTGGA GTTGAGTCCT 
ATGCAGAAGA AGTACTACAA GTATATTCTC ACGCGGAATT TCGAAGCACT TAATGCTCGA 
GGTGGTGGCA ACCAGGTTTC TCTGCTGAAC GTGGTGATGG ATCTTAAGAA ATGCTGCAAC 
CACCCTTATC TCTTCCCTGT GGCAGCAATG GAAGCCCCTA AGATGCCTAA TGGTATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAGTTGTTGC TGCTGCAGAA GATGCTTAAG 
AACCTGAAAG AGGGAGGGCA TCGTGTGCTC ATCTTCTCCC AGATGACCAA GATGTTGGAC 
TTGCTAGAGG ATTTTTTAGA ACATGAAGGT TATAAATATG AACGTATTGA TGGTGGAATC 
ACTGGGAACA TGCGTCAGGA GGCCATTGAT CGCTTCAATG CACCGGGTGC TCAACAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGGATCA ATCTGGCCAC TGCGGACACA 
GTTATTATAT ATGACTCTGA CTGGAACCCC CATAATGACA TCCAGGCCTT TAGCAGAGCC 
CACCGTATTG GGCAAAATAA GAAGGTGATG ATCTACCGGT TTGTGACCCG TGCATCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAGATGATGC TGACACATTT AGTGGTACGG 
CCTGGCCTGG GCTCCAAGAC AGGCTCAATG TCTAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAGCTATT CAAGGATGAA GCCACGGATG GAGGAGGAGA CAACAAGGAG 
GGAGAAGACA GCAGTGTTAT CCACTATGAC GATAAGGCCA TTGAACGACT GCTGGATCGA 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCTC AGTATGTGGT ACGGGAAGAA GAGATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATAA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCACAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCCTCA GAGGAAGGTG ATGAAGACTT TGACGAACGG 
TCAGAAGCTC CCCGCAGGCC CAGTCGCAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTA 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCAT TTCTTAATGC AATTATGCGA TATGGCATGC CACCTCAGGA TGCTTTTACC 
ACTCAGTGGC TTGTGAGAGA TCTTCGAGGC AAGTCAGAGA AAGAATTTAA GGCTTATGTG 
TCACTCTTCA TGCGACATTT GTGTGAGCCT GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGCGTCCCAC GAGAAGGCCT GTCTCGTCAA CATGTTCTCA CTAGGATCGG TGTCATGTCT 
CTGATTCGAA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GACGCTGGAG CATGCCCGAA 
CTGGCTGAAG TAGAGGAAAA CAAAAAAATG TCTCAGCCAG GTTCACCTTC TCCAAAGACT 
CCTACACCTT CCACACCAGG GGACACACAA CCCAATACTC CTGCGCCTGT GCCACCTGCT 
GAGGATGGGA TAAAAATAGA GGAAAATAGC CTTAAAGAAG AAGAGAGCAC AGAAGGAGAG 
AAGGAAGTTA AATCTACAGC CCCTGAGGCG ACTGTGGAGT GTGCACAGCC CCCTGCCCCT 
GCCCCTGCCA CTGCCCCTGC CACTGCCACT GCCCCTGAGG ATGACAAGGC TCCTGCTGAG 
CCTCCTGAGG GGGAGGAAAA AGTGGAAAAG GCAGAGGTGA AGGAGAGAAC AGAGGAGCCT 
ATGGAGACAG AGGCCAAAGG TACTACTGAG GTGGAGAAAG TGGAGGAGAA GTCTGCGGTA 
GACCTGACCC CGATCGTGGT GGAAGACAAA GAAGAGAAGA AGGAGGAGGA GGAGAAAAAG 
GACGTGATGC TTCAGAATGG AGAAACTCCC AAGGATCTTA GTGATGAGAA GCAGAAGAAA 
AACAGCAAAC AGCGTTTCAT GTTCAACATT GCAGATGGTG GTTTTACTGA GTTGCACTCA 
CTGTGGCAGA ATGAAGAGCG GGCAGCCACA GTCACTAAGA AGACTTACGA GATCTGGCAC 
CGTCGCCATG ATTACTGGCT GCTGGCTGGC ATTATAAACC ACGGCTATGC CCGGTGGCAG 
GACATCCAGA ATGACCCACG GTATGCCATC CTCAATGAGC CTTTCAAGGG TGAAATGAAT 
CGTGGCAATT TTCTAGAGAT CAAGAATAAA TTTTTAGCTC GAAGATTCAA GCTCTTAGAA 
CAAGCCCTGG TGATCGAGGA GCAGCTTCGC AGGGCAGCTT ACCTGAACAT GTCCGAGGAC 
CCCTCTCACC CGTCTATGGC CCTTAACACC CGCTTCGCTG AGGTGGAGTG TTTGGCAGAA 
AGTCACCAGC ACCTGTCCAA GGAGTCGATG GCAGGGAATA AGCCGGCCAA TGCAGTCCTA 
CACAAAGTCC TGAAACAGCT GGAGGAACTG TTGAGTGACA TGAAAGCTGA TGTGACGCGG 
CTCCCAGCCA CCATTGCCCG CATCCCCCCA GTCGCTGTGA GGTTACAGAT GTCAGAGCGG 
AACATTCTCA GCCGCCTGGC AAACAGGGCG CCAGAACCTC CCCCACAGCA GGTAGCCCAG 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Chd4
Entrez Gene ID	107932
Full Name	chromodomain helicase DNA binding protein 4
Synonyms	9530019N15Rik,AA617397,BC005710,D6Ertd380e,Mi-2beta,mKIAA4075
General protein information	Preferred Names chromodomain helicase DNA binding protein 4 Names chromodomain-helicase-DNA-binding protein 4 CHD-4 Mi-2 beta
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 F2\|6 59.28 cM

Mus musculus (house mouse)	Chd4	NP_666091.1
Caenorhabditis elegans (roundworm)	chd-3	NP_510140.1
Danio rerio (zebrafish)	chd4a	NP_001038323.1
Canis lupus familiaris (dog)	CHD4	XP_867754.2
Danio rerio (zebrafish)	chd4b	XP_685699.4
Arabidopsis thaliana (thale cress)	PKL	NP_565587.1
Arabidopsis thaliana (thale cress)	PKR2	NP_194918.2
Homo sapiens (human)	CHD4	NP_001264.2
Bos taurus (cattle)	CHD4	NP_001193430.1
Gallus gallus (chicken)	CHD4	XP_003640489.2
Caenorhabditis elegans (roundworm)	let-418	NP_504523.1
Xenopus tropicalis (tropical clawed frog)	chd4	NP_001096166.1
	CHD4	XP_001107252.2
Rattus norvegicus (Norway rat)	Chd4	XP_006237457.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Chd4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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