E2f4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	E2f4
Entrez Gene ID	104394
Full Name	E2F transcription factor 4
Synonyms	2010111M04Rik,AI427446
General protein information	Preferred Names E2F transcription factor 4 Names transcription factor E2F4 E2F-4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_148952.1	NP_683754.1	transcription factor E2F4

Pathways from BioSystems

Homologies

Homo sapiens (human)	E2F4	NP_001941.2
	E2F4	XP_001088067.1
Canis lupus familiaris (dog)	E2F4	XP_853975.2
Mus musculus (house mouse)	E2f4	NP_683754.1
Danio rerio (zebrafish)	e2f4	NP_998597.1
Rattus norvegicus (Norway rat)	E2f4	NP_001258274.1
Gallus gallus (chicken)	E2F4	NP_001005835.1
Xenopus tropicalis (tropical clawed frog)	e2f4	NP_001107966.1
Pan troglodytes (chimpanzee)	E2F4	XP_511025.4
Bos taurus (cattle)	E2F4	NP_001069341.1

Gene Ontology
Bibliography

Related articles in PubMed

Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.
Mori M, Hazan R, Danielian PS, Mahoney JE, Li H, Lu J, Miller ES, Zhu X, Lees JA, Cardoso WV
Nature communications815857(2017 Jul)

E2f4 and E2f5 are essential for the development of the male reproductive system.
Danielian PS, Hess RA, Lees JA
Cell cycle (Georgetown, Tex.)15(2)250-60(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

E2f4 and E2f5 display overlapping roles in controlling the normal development of the male reproductive system.
Title: E2f4 and E2f5 are essential for the development of the male reproductive system.

Following stress exposure, E2F4-p130 complexes are lost rapidly along with the presence of E2F4 at E2F-containing B-Myb promoter sites.
Title: Regulation of ischemic neuronal death by E2F4-p130 protein complexes.

E2F4 is part of a transcriptional repressor complex comprising p130 and HDAC1 in cardiac myocytes. Relief from E2F4-dependent gene silencing induces the expression of pro-apoptotic genes.
Title: Anti-apoptotic function of the E2F transcription factor 4 (E2F4)/p130, a member of retinoblastoma gene family in cardiac myocytes.

In terminally differentiated cells, common KDM5A and E2F4 gene targets were bound by the pRB-related protein p130, a DREAM complex component.
Title: Coordinated repression of cell cycle genes by KDM5A and E2F4 during differentiation.

We report here a new cellular function of p27 as a transcriptional regulator in association with p130/E2F4 complexes that could be relevant for tumorigenesis.
Title: p27Kip1 represses transcription by direct interaction with p130/E2F4 at the promoters of target genes.

The following E2f4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E2f4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02058	NM_148952.1 Latest version!	Mus musculus E2F transcription factor 4 (E2f4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E2f4 ( NM_148952.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_683754.1 Mus musculus E2F transcription factor 4 (E2f4), mRNA.

Price & Availability↑

CloneID	OMu02058
Clone ID Related Accession (Same CDS sequence)	NM_148952.1
Accession Version	NM_148952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	transcription factor E2F4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC026649.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026649.1, AK145950.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGG CCGGGCCACA GGCGCCGCCG CCCCCGGGGA CTCCAAGCCG GCACGAGAAG 
AGTCTGGGAC TTCTCACCAC CAAGTTCGTG TCGCTCTTGC AGGAAGCCAA GGACGGCGTG 
CTTGACCTCA AGCTGGCAGC CGACACTCTT GCTGTGCGCC AGAAACGGCG GATCTACGAC 
ATCACCAACG TGCTGGAAGG CATCGGTCTG ATCGAGAAGA AATCCAAGAA CAGCATCCAG 
TGGAAGGGCG TCGGGCCAGG TTGCAATACC CGGGAGATCG CTGACAAGCT GATTGAGCTC 
AAGGCAGAGA TCGAGGAGCT GCAGCAACGA GAGCAAGAAC TGGACCAGCA CAAGGTGTGG 
GTGCAGCAGA GCATCCGGAA TGTCACTGAG GACGTCCAGA ACAGCTGCTT GGCCTACGTG 
ACTCATGAAG ACATCTGCAG ATGCTTTGCT GGAGATACCC TCCTTGCCAT CCGGGCGCCA 
TCGGGCACCA GTCTAGAGGT GCCCATCCCA GAGGGTCTCA ATGGCCAGAA GAAGTACCAG 
ATTCACTTAA AGAGCATGAG TGGGCCTATC GAGGTGCTGC TAGTGAACAA GGAGGCCTGG 
AGTTCACCAC CTGTGGCTGT GCCTGTCCCT CCCCCTGACG ATCTACTCCA GAGTCCACCT 
GCTGTTTCTA CACCTCCACC TCTGCCCAAG CCTGCCTTAG CTCAACCCCA AGAATCCTCT 
CCTCCAAGCA GCCCCCAGCT AACTACCCCC ACTCCTGTCC TTGGCAGCAC TCAAGTCTCA 
GAGGTGGCAT GCCAGACATC TGAGATTGCA GTGAGTGGTA GCCCTGGAAC TGAGAACAAG 
GACAGTGGTG AAGTCAGCTC ACTCCCACTG GGCCTGACAG CACTGGACAC TCGGCCTCTG 
CAGTCCTCTG CACTGCTGGA TAGTAGTAGC AGCAGCAGCA GCAGCAGCAG TAGCAGCAGC 
AGCAGTTCAT CTGGACCCAA CCCTTCTACC TCCTTTGAGC CCATCAAAGC AGACCCCACA 
GGCGTTCTGG ATCTCCCCAA AGAGCTGTCA GAAATCTTCG ACCCCACGAG AGAGTGCATG 
AGCTCCGAGC TGCTGGAAGA ACTGATGTCT TCAGAAGTGT TTGCCCCCCT CCTCCGACTT 
TCTCCACCTC CCGGAGACCA CGATTACATC TACAACCTGG ACGAGAGTGA AGGTGTCTGT 
GATCTCTTTG ATGTTCCTGT TCTCAAACTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_683754.1
CDS	79..1311
Translation	MAEAGPQAPPPPGTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGLIEKKSKNSIQWKGVGPGCNTREIADKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYVTHEDICRCFAGDTLLAIRAPSGTSLEVPIPEGLNGQKKYQIHLKSMSGPIEVLLVNKEAWSSPPVAVPVPPPDDLLQSPPAVSTPPPLPKPALAQPQESSPPSSPQLTTPTPVLGSTQVSEVACQTSEIAVSGSPGTENKDSGEVSSLPLGLTALDTRPLQSSALLDSSSSSSSSSSSSSSSSSGPNPSTSFEPIKADPTGVLDLPKELSEIFDPTRECMSSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESEGVCDLFDVPVLKL

Target ORF information:

RefSeq Version	NM_148952.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus E2F transcription factor 4 (E2f4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148952.1

ORF Insert Sequence:

ATGGCGGAGG CCGGGCCACA GGCGCCGCCG CCCCCGGGGA CTCCAAGCCG GCACGAGAAG 
AGTCTGGGAC TTCTCACCAC CAAGTTCGTG TCGCTCTTGC AGGAAGCCAA GGACGGCGTG 
CTTGACCTCA AGCTGGCAGC CGACACTCTT GCTGTGCGCC AGAAACGGCG GATCTACGAC 
ATCACCAACG TGCTGGAAGG CATCGGTCTG ATCGAGAAGA AATCCAAGAA CAGCATCCAG 
TGGAAGGGCG TCGGGCCAGG TTGCAATACC CGGGAGATCG CTGACAAGCT GATTGAGCTC 
AAGGCAGAGA TCGAGGAGCT GCAGCAACGA GAGCAAGAAC TGGACCAGCA CAAGGTGTGG 
GTGCAGCAGA GCATCCGGAA TGTCACTGAG GACGTCCAGA ACAGCTGCTT GGCCTACGTG 
ACTCATGAAG ACATCTGCAG ATGCTTTGCT GGAGATACCC TCCTTGCCAT CCGGGCGCCA 
TCGGGCACCA GTCTAGAGGT GCCCATCCCA GAGGGTCTCA ATGGCCAGAA GAAGTACCAG 
ATTCACTTAA AGAGCATGAG TGGGCCTATC GAGGTGCTGC TAGTGAACAA GGAGGCCTGG 
AGTTCACCAC CTGTGGCTGT GCCTGTCCCT CCCCCTGACG ATCTACTCCA GAGTCCACCT 
GCTGTTTCTA CACCTCCACC TCTGCCCAAG CCTGCCTTAG CTCAACCCCA AGAATCCTCT 
CCTCCAAGCA GCCCCCAGCT AACTACCCCC ACTCCTGTCC TTGGCAGCAC TCAAGTCTCA 
GAGGTGGCAT GCCAGACATC TGAGATTGCA GTGAGTGGTA GCCCTGGAAC TGAGAACAAG 
GACAGTGGTG AAGTCAGCTC ACTCCCACTG GGCCTGACAG CACTGGACAC TCGGCCTCTG 
CAGTCCTCTG CACTGCTGGA TAGTAGTAGC AGCAGCAGCA GCAGCAGCAG TAGCAGCAGC 
AGCAGTTCAT CTGGACCCAA CCCTTCTACC TCCTTTGAGC CCATCAAAGC AGACCCCACA 
GGCGTTCTGG ATCTCCCCAA AGAGCTGTCA GAAATCTTCG ACCCCACGAG AGAGTGCATG 
AGCTCCGAGC TGCTGGAAGA ACTGATGTCT TCAGAAGTGT TTGCCCCCCT CCTCCGACTT 
TCTCCACCTC CCGGAGACCA CGATTACATC TACAACCTGG ACGAGAGTGA AGGTGTCTGT 
GATCTCTTTG ATGTTCCTGT TCTCAAACTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.
Nature communications815857(2017 Jul)
Mori M,Hazan R,Danielian PS,Mahoney JE,Li H,Lu J,Miller ES,Zhu X,Lees JA,Cardoso WV

E2f4 and E2f5 are essential for the development of the male reproductive system.
Cell cycle (Georgetown, Tex.)15(2)250-60(2016)
Danielian PS,Hess RA,Lees JA

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E2f4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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