AMY2B cDNA ORF clone, Miniopterus natalensis

Gene
Clones
gRNAs

Summary

Gene Symbol	AMY2B
Entrez Gene ID	107537180
Full Name	amylase, alpha 2B (pancreatic)
Gene Type	protein-coding
Organism	Miniopterus natalensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_016213127.1	XP_016068613.1	alpha-amylase 2B

Pathways from BioSystems
Gene Ontology

The following AMY2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AMY2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMi44466	XM_016213127.1 Latest version!	Miniopterus natalensis amylase, alpha 2B (pancreatic) (AMY2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AMY2B ( XM_016213127.1 ) cDNA ORF clone, Miniopterus natalensis -> XP_016068613.1 Miniopterus natalensis amylase, alpha 2B (pancreatic) (AMY2B), mRNA.

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CloneID	OMi44466
Clone ID Related Accession (Same CDS sequence)	XM_016213127.1
Accession Version	XM_016213127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Miniopterus natalensis
Product	alpha-amylase 2B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015504538.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Miniopterus natalensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTCC TTCTGCTGCT CTCAGCCCTT GGGCTCTGCT GGGCCCAGTG GGACCCCAAC 
ACCAGGCCGG GGCGCACGGT CATCGTGCAC CTGTTTGAGT GGCGCTGGGC CGACATCGCT 
CTCGAGTGCG AGCGATACTT GGCTCCCAGA GGATTCGGAG GGGTCCAGAT CTCTCCACCC 
AACGAAAACG TTATAATTAA TGACCCTTTA AGACCTTGGT GGGAAAGATA CCAACCGATT 
AGCTACAAGT TATGTACAAG ATCAGGAAAT GAAGATGAAT TCAGAGACAT GGTGAAGAGA 
TGCAACGACG TGGGCGTTCA CATCTACGTG GACGCCGTCA TCAACCACAT GTGTGGGAAC 
GGCGTGAGCG CGGGAACCAG GAGCACCTGC GGGAGCTTCT TCAACCCGGG AGCCAGGGAC 
TTTCCCTCCG TCCCCTACTC CGCTTGGGAC TTTAACGACC ATAAATGCAG AACTGCCAGT 
GGCGACATTG AGAACTACAA CGATGCTTAT CAGGTCCGAG ACTGTCGCCT GGTCGGGCTG 
CTTGACCTGG CCCTAGAGAA GGACTACGTG CGGTCCACGG TGGCCGAGTA CATGAACCGC 
CTCATCGACA TCGGCGTGGC AGGCTTCCGA ATCGACGCTG CCAAGCACAT GTGGCCCGGA 
GACATGAAGG CCGTCCTGGA TAAGCTGCGC CATCTGAATA CAACCTGGTT CCCAGCAGAA 
ACGAAGCCTT TCATTTACCA GGAGGTAATT GATCTGGGCG GTGAGTCCAT TAAAAGCAGT 
GAATACTTTG ACAACGGCTT TGTGACGGAG TTCAAGTACG GAGCAAAGCT GGGCACAGTG 
CTGCGCAAGT GGAGCGGAGA GAGGATGGCC TACCTCAAGA ACTGGGGAGA AGGCTGGGGC 
TTCATGCCTT CCAACAGAGC GCTCGTCTTT GTGGACAACC ATGACAACCA GCGGGGCCAC 
GGCGCGGGGG GCGCGTCCAT CCTCACGTTC TGGGACTCGA GACTCTACAA AATGGGAGTA 
GCATTTATGC TCGCCCATCC TTACGGGTTC ACGCGCGTCA TGTCAAGCTT CCATTGGCCA 
AGGTATTTTG TGAATGGAAA AGATATTAAC GACTGGATGG GGCCACCGAA CGATGATGGG 
GTGATTAAAG AGGTCACCAT CAACCCGGAC ACCACCTGTG GCAACGGCTG GGTCTGTGAG 
CACCGGTGGC GCCAGATAAG GAACATGGTC ACGTTCCGCA ACGTGGCGGA CGGCCAGCCC 
TTCGCCAACT GGTGGGATAA CGGCAGCAAC CAGGTCGCCT TCGGGAGAGG GAACAAGGGC 
TTCATCGTCT TCAACAATGA CGAGTGGCCA TTAAGTGCAT ATTTGCAAAC TGGTCTTCCT 
GCTGGCACAT ACTGCGATGT GATTTCCGGA GATAAAATTG ATAACAAATG TACAGGAATG 
AAGATCTACG TCTCTGCTGA TGGCAATGCT CACTTTTCTA TCAGCAACTC TGCCGAAGAT 
CCGTTTATCG CTATTCATGA GGAGTCTAAA TTAGAAAACT TGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016068613.1
CDS	1723..3270
Translation	MKFLLLLSALGLCWAQWDPNTRPGRTVIVHLFEWRWADIALECERYLAPRGFGGVQISPPNENVIINDPLRPWWERYQPISYKLCTRSGNEDEFRDMVKRCNDVGVHIYVDAVINHMCGNGVSAGTRSTCGSFFNPGARDFPSVPYSAWDFNDHKCRTASGDIENYNDAYQVRDCRLVGLLDLALEKDYVRSTVAEYMNRLIDIGVAGFRIDAAKHMWPGDMKAVLDKLRHLNTTWFPAETKPFIYQEVIDLGGESIKSSEYFDNGFVTEFKYGAKLGTVLRKWSGERMAYLKNWGEGWGFMPSNRALVFVDNHDNQRGHGAGGASILTFWDSRLYKMGVAFMLAHPYGFTRVMSSFHWPRYFVNGKDINDWMGPPNDDGVIKEVTINPDTTCGNGWVCEHRWRQIRNMVTFRNVADGQPFANWWDNGSNQVAFGRGNKGFIVFNNDEWPLSAYLQTGLPAGTYCDVISGDKIDNKCTGMKIYVSADGNAHFSISNSAEDPFIAIHEESKLENLH

Target ORF information:

RefSeq Version	XM_016213127.1
Organism	Miniopterus natalensis
Definition	Miniopterus natalensis amylase, alpha 2B (pancreatic) (AMY2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016213127.1

ORF Insert Sequence:

ATGAAGTTCC TTCTGCTGCT CTCAGCCCTT GGGCTCTGCT GGGCCCAGTG GGACCCCAAC 
ACCAGGCCGG GGCGCACGGT CATCGTGCAC CTGTTTGAGT GGCGCTGGGC CGACATCGCT 
CTCGAGTGCG AGCGATACTT GGCTCCCAGA GGATTCGGAG GGGTCCAGAT CTCTCCACCC 
AACGAAAACG TTATAATTAA TGACCCTTTA AGACCTTGGT GGGAAAGATA CCAACCGATT 
AGCTACAAGT TATGTACAAG ATCAGGAAAT GAAGATGAAT TCAGAGACAT GGTGAAGAGA 
TGCAACGACG TGGGCGTTCA CATCTACGTG GACGCCGTCA TCAACCACAT GTGTGGGAAC 
GGCGTGAGCG CGGGAACCAG GAGCACCTGC GGGAGCTTCT TCAACCCGGG AGCCAGGGAC 
TTTCCCTCCG TCCCCTACTC CGCTTGGGAC TTTAACGACC ATAAATGCAG AACTGCCAGT 
GGCGACATTG AGAACTACAA CGATGCTTAT CAGGTCCGAG ACTGTCGCCT GGTCGGGCTG 
CTTGACCTGG CCCTAGAGAA GGACTACGTG CGGTCCACGG TGGCCGAGTA CATGAACCGC 
CTCATCGACA TCGGCGTGGC AGGCTTCCGA ATCGACGCTG CCAAGCACAT GTGGCCCGGA 
GACATGAAGG CCGTCCTGGA TAAGCTGCGC CATCTGAATA CAACCTGGTT CCCAGCAGAA 
ACGAAGCCTT TCATTTACCA GGAGGTAATT GATCTGGGCG GTGAGTCCAT TAAAAGCAGT 
GAATACTTTG ACAACGGCTT TGTGACGGAG TTCAAGTACG GAGCAAAGCT GGGCACAGTG 
CTGCGCAAGT GGAGCGGAGA GAGGATGGCC TACCTCAAGA ACTGGGGAGA AGGCTGGGGC 
TTCATGCCTT CCAACAGAGC GCTCGTCTTT GTGGACAACC ATGACAACCA GCGGGGCCAC 
GGCGCGGGGG GCGCGTCCAT CCTCACGTTC TGGGACTCGA GACTCTACAA AATGGGAGTA 
GCATTTATGC TCGCCCATCC TTACGGGTTC ACGCGCGTCA TGTCAAGCTT CCATTGGCCA 
AGGTATTTTG TGAATGGAAA AGATATTAAC GACTGGATGG GGCCACCGAA CGATGATGGG 
GTGATTAAAG AGGTCACCAT CAACCCGGAC ACCACCTGTG GCAACGGCTG GGTCTGTGAG 
CACCGGTGGC GCCAGATAAG GAACATGGTC ACGTTCCGCA ACGTGGCGGA CGGCCAGCCC 
TTCGCCAACT GGTGGGATAA CGGCAGCAAC CAGGTCGCCT TCGGGAGAGG GAACAAGGGC 
TTCATCGTCT TCAACAATGA CGAGTGGCCA TTAAGTGCAT ATTTGCAAAC TGGTCTTCCT 
GCTGGCACAT ACTGCGATGT GATTTCCGGA GATAAAATTG ATAACAAATG TACAGGAATG 
AAGATCTACG TCTCTGCTGA TGGCAATGCT CACTTTTCTA TCAGCAACTC TGCCGAAGAT 
CCGTTTATCG CTATTCATGA GGAGTCTAAA TTAGAAAACT TGCATTAA