N4BP2 gene cDNA ORF clone, Microcebus murinus(gray mouse lemur)

mRNA	Protein	Name
XM_012753930.2	XP_012609384.1	NEDD4-binding protein 2 isoform X1
XM_012753931.2	XP_012609385.1	NEDD4-binding protein 2 isoform X1
XM_020282433.1	XP_020138022.1	NEDD4-binding protein 2 isoform X1
XM_012753948.2	XP_012609402.1	NEDD4-binding protein 2 isoform X1
XM_012753950.2	XP_012609404.1	NEDD4-binding protein 2 isoform X1
XM_012753961.2	XP_012609415.1	NEDD4-binding protein 2 isoform X3
XM_012753965.2	XP_012609419.1	NEDD4-binding protein 2 isoform X3
XM_012753939.1	XP_012609393.1	NEDD4-binding protein 2 isoform X1
XM_012753941.2	XP_012609395.1	NEDD4-binding protein 2 isoform X1
XM_012753952.2	XP_012609406.1	NEDD4-binding protein 2 isoform X1
XM_012753936.2	XP_012609390.1	NEDD4-binding protein 2 isoform X1
XM_012753937.2	XP_012609391.1	NEDD4-binding protein 2 isoform X1
XM_012753940.2	XP_012609394.1	NEDD4-binding protein 2 isoform X1
XM_012753943.2	XP_012609397.1	NEDD4-binding protein 2 isoform X1
XM_012753945.2	XP_012609399.1	NEDD4-binding protein 2 isoform X1
XM_012753951.2	XP_012609405.1	NEDD4-binding protein 2 isoform X1
XM_012753956.2	XP_012609410.1	NEDD4-binding protein 2 isoform X3
XM_012753932.1	XP_012609386.1	NEDD4-binding protein 2 isoform X1
XM_012753933.2	XP_012609387.1	NEDD4-binding protein 2 isoform X1
XM_012753934.1	XP_012609388.1	NEDD4-binding protein 2 isoform X1
XM_012753942.2	XP_012609396.1	NEDD4-binding protein 2 isoform X1
XM_012753949.1	XP_012609403.1	NEDD4-binding protein 2 isoform X1
XM_012753953.1	XP_012609407.1	NEDD4-binding protein 2 isoform X1
XM_012753954.2	XP_012609408.1	NEDD4-binding protein 2 isoform X2
XM_012753955.2	XP_012609409.1	NEDD4-binding protein 2 isoform X2
XM_012753958.2	XP_012609412.1	NEDD4-binding protein 2 isoform X3
XM_012753959.1	XP_012609413.1	NEDD4-binding protein 2 isoform X3
XM_012753963.2	XP_012609417.1	NEDD4-binding protein 2 isoform X3
XM_012753964.1	XP_012609418.1	NEDD4-binding protein 2 isoform X3
XM_012753938.1	XP_012609392.1	NEDD4-binding protein 2 isoform X1
XM_012753944.1	XP_012609398.1	NEDD4-binding protein 2 isoform X1
XM_012753947.2	XP_012609401.1	NEDD4-binding protein 2 isoform X1
XM_012753957.2	XP_012609411.1	NEDD4-binding protein 2 isoform X3
XM_012753960.1	XP_012609414.1	NEDD4-binding protein 2 isoform X3
XM_012753966.2	XP_012609420.1	NEDD4-binding protein 2 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMi70581	XM_012753945.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753930.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753944.1 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753931.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753940.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753941.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753942.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753943.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753952.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753938.1 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753939.1 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753953.1 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753936.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753937.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753933.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753949.1 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753932.1 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753947.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753950.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_020282433.1 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753948.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753951.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70581	XM_012753934.1 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70582	XM_012753955.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70582	XM_012753954.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70583	XM_012753960.1 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70583	XM_012753959.1 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70583	XM_012753961.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70583	XM_012753957.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70583	XM_012753958.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70583	XM_012753956.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70583	XM_012753964.1 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70583	XM_012753963.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70583	XM_012753966.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMi70583	XM_012753965.2 Latest version!	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

N4BP2 ( XM_012753930.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609384.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753930.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753930.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609384.1
CDS	1361..6676
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753930.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753930.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753931.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609385.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X2, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609385.1
CDS	1397..6712
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753931.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753931.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_020282433.1 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_020138022.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X10, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_020282433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020138022.1
CDS	1571..6886
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_020282433.1
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020282433.1

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753948.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609402.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X18, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753948.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753948.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609402.1
CDS	1361..6676
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753948.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753948.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753950.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609404.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X20, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753950.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753950.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609404.1
CDS	444..5759
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753950.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753950.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753939.1 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609393.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X9, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753939.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609393.1
CDS	551..5866
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753939.1
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753939.1

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753941.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609395.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X12, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753941.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753941.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609395.1
CDS	1460..6775
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753941.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753941.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753952.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609406.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X22, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609406.1
CDS	260..5575
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753952.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753952.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753936.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609390.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X6, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753936.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609390.1
CDS	622..5937
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753936.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753936.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753937.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609391.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X7, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753937.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753937.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609391.1
CDS	438..5753
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753937.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753937.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753940.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609394.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X11, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753940.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753940.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609394.1
CDS	1486..6801
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753940.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753940.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753943.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609397.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X14, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753943.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753943.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609397.1
CDS	1297..6612
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753943.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753943.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753945.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609399.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X16, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753945.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609399.1
CDS	1263..6578
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753945.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753945.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753951.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609405.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X21, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753951.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753951.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609405.1
CDS	211..5526
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753951.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753951.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753932.1 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609386.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X3, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609386.1
CDS	1185..6500
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753932.1
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753932.1

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753933.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609387.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X4, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753933.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753933.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609387.1
CDS	1281..6596
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753933.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753933.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753934.1 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609388.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X5, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609388.1
CDS	445..5760
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753934.1
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753934.1

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753942.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609396.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X13, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753942.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753942.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609396.1
CDS	1361..6676
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753942.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753942.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753949.1 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609403.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X19, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609403.1
CDS	267..5582
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753949.1
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753949.1

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753953.1 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609407.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X23, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609407.1
CDS	373..5688
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753953.1
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753953.1

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753938.1 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609392.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X8, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609392.1
CDS	390..5705
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753938.1
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753938.1

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753944.1 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609398.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X15, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753944.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609398.1
CDS	570..5885
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753944.1
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753944.1

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753947.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609401.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X17, mRNA.

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CloneID	OMi70581
Clone ID Related Accession (Same CDS sequence)	XM_012753930.2 , XM_012753931.2 , XM_020282433.1 , XM_012753948.2 , XM_012753950.2 , XM_012753939.1 , XM_012753941.2 , XM_012753952.2 , XM_012753936.2 , XM_012753937.2 , XM_012753940.2 , XM_012753943.2 , XM_012753945.2 , XM_012753951.2 , XM_012753932.1 , XM_012753933.2 , XM_012753934.1 , XM_012753942.2 , XM_012753949.1 , XM_012753953.1 , XM_012753938.1 , XM_012753944.1 , XM_012753947.2
Accession Version	XM_012753947.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753947.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609401.1
CDS	1183..6498
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753947.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753947.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753954.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609408.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X24, mRNA.

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CloneID	OMi70582
Clone ID Related Accession (Same CDS sequence)	XM_012753954.2 , XM_012753955.2
Accession Version	XM_012753954.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGGCG TAACATTCAT 
GGTGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACACT ATCAACGTTT TGTTTCAGTG 
CCAATAATCA TGAGTTCTTC GGTTCCAGAG AAAATTGAAC GTATTGAGTT GTGTGCATAT 
TCTTGTGAGG ATAAAAGTAC TGGCCCAAGA GAAAATGAAG ATATCACCTC TGAAAAAGAA 
GAAAATGTTT TATCCTCATC GTTGAAGCAC CTAGAGTTAA TTGAAGAGAA GAGTCTTGAA 
GTGACCAAAG AAACTATGTT ACCTGAGAAT GTTACGTATC TCCCTGATGC AGATTTAAAC 
AAAGGAAGAA AAGAAATAAG TGGTATGAGG CAAAACATTC AAAGTACTAA CATTCAGGAA 
GTTCCACACA TGTATTTTTC TGACTCTGAA AGCAAAGTAA GAGTGATGGA CAAAAGTGAA 
GAAGAAGTAG AAATGTCATC TGAAAAAGAG TGTGGTAAAA CTGATGCAGA TAGTTTTGTT 
GAGAGAGTAT CACTGAGTGT TTGCTATGAT GAAAATAATC AAGAAAATTG TGATCTTGTA 
AATACTGTAT CCCTTCAAAA CGAAAAATCT TCACCTGGCG AAACATTAGA AGAAAGAGCA 
ACAGTAAAGA AAAAAGCCTT TGGGAAACAA AAAAGTAAAT TGACTTTGGA AAAGTTCCCA 
AGACATGAGC TATCAGATTT TGTTGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG 
ACAAAAAGGA ACCGAAAAAC TGAAAAAACA TCTGTACAAA GTGACAGAAA GTATAATTGC 
CCTCAGTCAT GCAAAGTATT AGATAATAGT GTATCTGTGA ATATAGATTG TATCCAGAAA 
CAAGGATCTC CACACGAAAG TGTAAAGGAT GGCAAGAAGT CACAGGGTGA TGCTGTTTCA 
GAGTCACTCA ATAGCTGTAA ATGCAATCCT TGTAAAAATA CTGAAAAAAA CTCATTCAGC 
ATTGTGGGAG ATTGGCCTTC ATCTGACTCT TTAGCTCCGA GGGAGCACAG ATCAAGAATG 
ACAAAGACTG GTTTAAATGA ACCCAACCTA GAACTTGGAA CAAATGACAA TATGAGTGAA 
ATATTTTTAT ACACAGCACG TGAGGCCTGT TGGGACACAA ACCTTGAAAA ACTAAGAACA 
TTGGGCAGCT CCAGTCTAGA AAATTCCGAA ATGCTACCCA GTGAAATGAG CTGTGAGAGT 
CAGAGTTGTC TAAGTAAAAA GATTCATGGG CAATACACGT TGCCTCTTAC CAATAGCGCA 
CCAACTATTC CTGGAATAGT AGGACCACAA ACTTCAGCTG AATTTCCAGA GGGAATGCCT 
CAAGGAGTCC CTGGAATAGA AGTAGGCATA TGTACTCAGA CTGAACCACA GGATTTTGCT 
CTCTTATGGA AAATAGAAAA GAATAAAATT AGTATTTCAG ATTCTGTCAA AGTATTAACA 
GGAAGATTAG ATGGATTTAA GCCGAAAGTT TTCAATATTA ACACAAACTC AGACGTTCAA 
GAAACAATTC CATATAGGGT GATGTATGAT AAAAGCACAT ATGTTGAAGA AAGTGAGCTT 
ACCAGTGCTG ATGAATCTGA AAATCTTAAC ATTCTTTGTA AGCTATTTGG ATCTGTTTCA 
TTAGAAGCCC TAAAAGACTT ATATGAGAGG TGTAATAAAG ATATTATCTG GGCCACAAGC 
CTTTTGTTGG ATTCTGAAAC TAAATTATGT GAAGATACTG AATTTGAGAA TTTCCAGAAA 
TCATATGATG AATCACAAAT TGGGCCATTT TCTCTGGGAT TGAATTTGAA AGAAATTATT 
AGCCACAGGG GAACTTTAGA GGATTCTAAT TCTTCCTTGC CGGAATTTAG CCATGGATTT 
GCTGTCAGTA ACACTAATAC ACAGTCCACC TGTGATGCAG AAAATGAAAA CTCAGAGCAG 
GCAGAAATAA GAGCTATAAC TCCTGAAAAA CCTGATTTAA TAACAAGTAT ATTTCCTAGT 
GCTGCTGTAG ATCTAAAGAA TAATAGTGAA ACACTTCCTA ACAGTCGGGC AGAACTTTTA 
CATAGCATTA AGCAGACTTT TCCAGGTATT TCAAAAGCTA CTACCCCTAA AGATGTGTGT 
GAAATGGAGA AAAATCTAGT AATCACAGAG ACTGGAGACA ATATACATTC TTCTTTAAAT 
CTGTCTATTT TAAACTCTGT GTCAAGCACT TCAAATCTTG AATTAAATGA AGAAATTTAT 
TTTATGGACT CTATTGAAAT AAAGAAAAAC AAAAATCTTC CAAAGGATTA TATGAAATTT 
CCAAACACAG AAGAATTTAT GAATGAAGAT GAGCAGGAAA TGGAGAAAAT TCTAATGGCA 
GGGAGTAGTT TGTCAGCTGG AGTTAGTGAA GAAGATAAAA CTGAAATATT GAATCCCACT 
CCAGTGATGA CCAAATCTCT AACCATAGAC TGTCTGGAAT TGGCATTACC CCCTGAACTG 
GCTTTTCAAC TCAATGAATT ATTTGGCCCT GTTGGTATTG ATTCAGGATC TCTAACAGTT 
GAGGATTGTG TGGTTCATAT AGATCTGAAT CTGGCTAAAG TGATTCATGA GAAATGGAAA 
GAATCTGTGA TGGAGCGACA AAGACAAGAA GAGGTATCTT GTGGCAAGTT TAAGCGAGAT 
TCTTCCCTGG TTGGACATAT TGGTCTTGAT AATCCAGAAC AAAAATCATC TCAGAGAACA 
GGCAAAAAAT TACTGAAGAC TTTAGCAGCA TCTGAAATGC TACCTTTATT GGATCATTGG 
AATACTCAAA CTAAAAAAGT ATCACTAAGA GAAATAATGT CAGAAGAAAT TGCCTTACAG 
GAGAAACATG ATCTGAAAAG GGAGACACTT ATGTTTGAAA AAGATTGTGC CACTAAACTA 
AAGGAGAAGC AGCTCTTTAA GATATTTCCA GCCATTAACC AAAATTTCCT GGTGGACATT 
TTCAAGGACC ACAACTATTC ATTAGAACAC ACAGTACAAT TTCTAAACTG TGTTCTTGAA 
GGGGACCCTG TAAAAACAGT TGTAGCACAA GAGTTTGTTC ACCAAAATGA GAATGTCACT 
TCTCATACTA CCCAGAAGTC TAAAGAGAAA AAGGCAAAGA AATCAAAAGA GTCTGAAGAT 
ACACCAAGCG AACCATCTTT TCAGGATTTC GAGTACCCAG AATATGACGA CTATAGGGCA 
GAGGCTTTCC TGCACCAACA GAAGAGGATA GAATGCTACA GTAAGGCAAA AGAAGCTTAT 
CGGATGGGGA AGAAAAATGT TGCCACCTTT TACGCCCAAC AGGGTAGTCT TCATGAGCAG 
AAGATGAAAG AAGCCAATCA CCTTGCTGCT GTGGAGATCT TTGAGAAAGT CAATGCCTCG 
CTGCTGCCAC AGAATGTTTT AGACCTCCAT GGGCTGCATG TGGATGAAGC TATAGAACAA 
TTGATGACAG TTTTACAGCA GAAAACTGAA GAATTTAAGC AGAATGGTGG TAAGCCCTAT 
CTGTCTGTGA TCACGGGGAG AGGAAACCAC AGCCAGGGAG GAGTTGCTCG CATCAAGCCA 
GCCGTCATTA AATACCTCAC AAGCCATAGC TTCAGGTTCT CTGAAATTAA ACCAGGGTGC 
TTGAAAGTCA TGCTAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609408.1
CDS	1361..6661
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELARRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753954.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753954.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGGCG TAACATTCAT 
GGTGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACACT ATCAACGTTT TGTTTCAGTG 
CCAATAATCA TGAGTTCTTC GGTTCCAGAG AAAATTGAAC GTATTGAGTT GTGTGCATAT 
TCTTGTGAGG ATAAAAGTAC TGGCCCAAGA GAAAATGAAG ATATCACCTC TGAAAAAGAA 
GAAAATGTTT TATCCTCATC GTTGAAGCAC CTAGAGTTAA TTGAAGAGAA GAGTCTTGAA 
GTGACCAAAG AAACTATGTT ACCTGAGAAT GTTACGTATC TCCCTGATGC AGATTTAAAC 
AAAGGAAGAA AAGAAATAAG TGGTATGAGG CAAAACATTC AAAGTACTAA CATTCAGGAA 
GTTCCACACA TGTATTTTTC TGACTCTGAA AGCAAAGTAA GAGTGATGGA CAAAAGTGAA 
GAAGAAGTAG AAATGTCATC TGAAAAAGAG TGTGGTAAAA CTGATGCAGA TAGTTTTGTT 
GAGAGAGTAT CACTGAGTGT TTGCTATGAT GAAAATAATC AAGAAAATTG TGATCTTGTA 
AATACTGTAT CCCTTCAAAA CGAAAAATCT TCACCTGGCG AAACATTAGA AGAAAGAGCA 
ACAGTAAAGA AAAAAGCCTT TGGGAAACAA AAAAGTAAAT TGACTTTGGA AAAGTTCCCA 
AGACATGAGC TATCAGATTT TGTTGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG 
ACAAAAAGGA ACCGAAAAAC TGAAAAAACA TCTGTACAAA GTGACAGAAA GTATAATTGC 
CCTCAGTCAT GCAAAGTATT AGATAATAGT GTATCTGTGA ATATAGATTG TATCCAGAAA 
CAAGGATCTC CACACGAAAG TGTAAAGGAT GGCAAGAAGT CACAGGGTGA TGCTGTTTCA 
GAGTCACTCA ATAGCTGTAA ATGCAATCCT TGTAAAAATA CTGAAAAAAA CTCATTCAGC 
ATTGTGGGAG ATTGGCCTTC ATCTGACTCT TTAGCTCCGA GGGAGCACAG ATCAAGAATG 
ACAAAGACTG GTTTAAATGA ACCCAACCTA GAACTTGGAA CAAATGACAA TATGAGTGAA 
ATATTTTTAT ACACAGCACG TGAGGCCTGT TGGGACACAA ACCTTGAAAA ACTAAGAACA 
TTGGGCAGCT CCAGTCTAGA AAATTCCGAA ATGCTACCCA GTGAAATGAG CTGTGAGAGT 
CAGAGTTGTC TAAGTAAAAA GATTCATGGG CAATACACGT TGCCTCTTAC CAATAGCGCA 
CCAACTATTC CTGGAATAGT AGGACCACAA ACTTCAGCTG AATTTCCAGA GGGAATGCCT 
CAAGGAGTCC CTGGAATAGA AGTAGGCATA TGTACTCAGA CTGAACCACA GGATTTTGCT 
CTCTTATGGA AAATAGAAAA GAATAAAATT AGTATTTCAG ATTCTGTCAA AGTATTAACA 
GGAAGATTAG ATGGATTTAA GCCGAAAGTT TTCAATATTA ACACAAACTC AGACGTTCAA 
GAAACAATTC CATATAGGGT GATGTATGAT AAAAGCACAT ATGTTGAAGA AAGTGAGCTT 
ACCAGTGCTG ATGAATCTGA AAATCTTAAC ATTCTTTGTA AGCTATTTGG ATCTGTTTCA 
TTAGAAGCCC TAAAAGACTT ATATGAGAGG TGTAATAAAG ATATTATCTG GGCCACAAGC 
CTTTTGTTGG ATTCTGAAAC TAAATTATGT GAAGATACTG AATTTGAGAA TTTCCAGAAA 
TCATATGATG AATCACAAAT TGGGCCATTT TCTCTGGGAT TGAATTTGAA AGAAATTATT 
AGCCACAGGG GAACTTTAGA GGATTCTAAT TCTTCCTTGC CGGAATTTAG CCATGGATTT 
GCTGTCAGTA ACACTAATAC ACAGTCCACC TGTGATGCAG AAAATGAAAA CTCAGAGCAG 
GCAGAAATAA GAGCTATAAC TCCTGAAAAA CCTGATTTAA TAACAAGTAT ATTTCCTAGT 
GCTGCTGTAG ATCTAAAGAA TAATAGTGAA ACACTTCCTA ACAGTCGGGC AGAACTTTTA 
CATAGCATTA AGCAGACTTT TCCAGGTATT TCAAAAGCTA CTACCCCTAA AGATGTGTGT 
GAAATGGAGA AAAATCTAGT AATCACAGAG ACTGGAGACA ATATACATTC TTCTTTAAAT 
CTGTCTATTT TAAACTCTGT GTCAAGCACT TCAAATCTTG AATTAAATGA AGAAATTTAT 
TTTATGGACT CTATTGAAAT AAAGAAAAAC AAAAATCTTC CAAAGGATTA TATGAAATTT 
CCAAACACAG AAGAATTTAT GAATGAAGAT GAGCAGGAAA TGGAGAAAAT TCTAATGGCA 
GGGAGTAGTT TGTCAGCTGG AGTTAGTGAA GAAGATAAAA CTGAAATATT GAATCCCACT 
CCAGTGATGA CCAAATCTCT AACCATAGAC TGTCTGGAAT TGGCATTACC CCCTGAACTG 
GCTTTTCAAC TCAATGAATT ATTTGGCCCT GTTGGTATTG ATTCAGGATC TCTAACAGTT 
GAGGATTGTG TGGTTCATAT AGATCTGAAT CTGGCTAAAG TGATTCATGA GAAATGGAAA 
GAATCTGTGA TGGAGCGACA AAGACAAGAA GAGGTATCTT GTGGCAAGTT TAAGCGAGAT 
TCTTCCCTGG TTGGACATAT TGGTCTTGAT AATCCAGAAC AAAAATCATC TCAGAGAACA 
GGCAAAAAAT TACTGAAGAC TTTAGCAGCA TCTGAAATGC TACCTTTATT GGATCATTGG 
AATACTCAAA CTAAAAAAGT ATCACTAAGA GAAATAATGT CAGAAGAAAT TGCCTTACAG 
GAGAAACATG ATCTGAAAAG GGAGACACTT ATGTTTGAAA AAGATTGTGC CACTAAACTA 
AAGGAGAAGC AGCTCTTTAA GATATTTCCA GCCATTAACC AAAATTTCCT GGTGGACATT 
TTCAAGGACC ACAACTATTC ATTAGAACAC ACAGTACAAT TTCTAAACTG TGTTCTTGAA 
GGGGACCCTG TAAAAACAGT TGTAGCACAA GAGTTTGTTC ACCAAAATGA GAATGTCACT 
TCTCATACTA CCCAGAAGTC TAAAGAGAAA AAGGCAAAGA AATCAAAAGA GTCTGAAGAT 
ACACCAAGCG AACCATCTTT TCAGGATTTC GAGTACCCAG AATATGACGA CTATAGGGCA 
GAGGCTTTCC TGCACCAACA GAAGAGGATA GAATGCTACA GTAAGGCAAA AGAAGCTTAT 
CGGATGGGGA AGAAAAATGT TGCCACCTTT TACGCCCAAC AGGGTAGTCT TCATGAGCAG 
AAGATGAAAG AAGCCAATCA CCTTGCTGCT GTGGAGATCT TTGAGAAAGT CAATGCCTCG 
CTGCTGCCAC AGAATGTTTT AGACCTCCAT GGGCTGCATG TGGATGAAGC TATAGAACAA 
TTGATGACAG TTTTACAGCA GAAAACTGAA GAATTTAAGC AGAATGGTGG TAAGCCCTAT 
CTGTCTGTGA TCACGGGGAG AGGAAACCAC AGCCAGGGAG GAGTTGCTCG CATCAAGCCA 
GCCGTCATTA AATACCTCAC AAGCCATAGC TTCAGGTTCT CTGAAATTAA ACCAGGGTGC 
TTGAAAGTCA TGCTAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753955.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609409.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X25, mRNA.

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CloneID	OMi70582
Clone ID Related Accession (Same CDS sequence)	XM_012753954.2 , XM_012753955.2
Accession Version	XM_012753955.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGGCG TAACATTCAT 
GGTGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACACT ATCAACGTTT TGTTTCAGTG 
CCAATAATCA TGAGTTCTTC GGTTCCAGAG AAAATTGAAC GTATTGAGTT GTGTGCATAT 
TCTTGTGAGG ATAAAAGTAC TGGCCCAAGA GAAAATGAAG ATATCACCTC TGAAAAAGAA 
GAAAATGTTT TATCCTCATC GTTGAAGCAC CTAGAGTTAA TTGAAGAGAA GAGTCTTGAA 
GTGACCAAAG AAACTATGTT ACCTGAGAAT GTTACGTATC TCCCTGATGC AGATTTAAAC 
AAAGGAAGAA AAGAAATAAG TGGTATGAGG CAAAACATTC AAAGTACTAA CATTCAGGAA 
GTTCCACACA TGTATTTTTC TGACTCTGAA AGCAAAGTAA GAGTGATGGA CAAAAGTGAA 
GAAGAAGTAG AAATGTCATC TGAAAAAGAG TGTGGTAAAA CTGATGCAGA TAGTTTTGTT 
GAGAGAGTAT CACTGAGTGT TTGCTATGAT GAAAATAATC AAGAAAATTG TGATCTTGTA 
AATACTGTAT CCCTTCAAAA CGAAAAATCT TCACCTGGCG AAACATTAGA AGAAAGAGCA 
ACAGTAAAGA AAAAAGCCTT TGGGAAACAA AAAAGTAAAT TGACTTTGGA AAAGTTCCCA 
AGACATGAGC TATCAGATTT TGTTGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG 
ACAAAAAGGA ACCGAAAAAC TGAAAAAACA TCTGTACAAA GTGACAGAAA GTATAATTGC 
CCTCAGTCAT GCAAAGTATT AGATAATAGT GTATCTGTGA ATATAGATTG TATCCAGAAA 
CAAGGATCTC CACACGAAAG TGTAAAGGAT GGCAAGAAGT CACAGGGTGA TGCTGTTTCA 
GAGTCACTCA ATAGCTGTAA ATGCAATCCT TGTAAAAATA CTGAAAAAAA CTCATTCAGC 
ATTGTGGGAG ATTGGCCTTC ATCTGACTCT TTAGCTCCGA GGGAGCACAG ATCAAGAATG 
ACAAAGACTG GTTTAAATGA ACCCAACCTA GAACTTGGAA CAAATGACAA TATGAGTGAA 
ATATTTTTAT ACACAGCACG TGAGGCCTGT TGGGACACAA ACCTTGAAAA ACTAAGAACA 
TTGGGCAGCT CCAGTCTAGA AAATTCCGAA ATGCTACCCA GTGAAATGAG CTGTGAGAGT 
CAGAGTTGTC TAAGTAAAAA GATTCATGGG CAATACACGT TGCCTCTTAC CAATAGCGCA 
CCAACTATTC CTGGAATAGT AGGACCACAA ACTTCAGCTG AATTTCCAGA GGGAATGCCT 
CAAGGAGTCC CTGGAATAGA AGTAGGCATA TGTACTCAGA CTGAACCACA GGATTTTGCT 
CTCTTATGGA AAATAGAAAA GAATAAAATT AGTATTTCAG ATTCTGTCAA AGTATTAACA 
GGAAGATTAG ATGGATTTAA GCCGAAAGTT TTCAATATTA ACACAAACTC AGACGTTCAA 
GAAACAATTC CATATAGGGT GATGTATGAT AAAAGCACAT ATGTTGAAGA AAGTGAGCTT 
ACCAGTGCTG ATGAATCTGA AAATCTTAAC ATTCTTTGTA AGCTATTTGG ATCTGTTTCA 
TTAGAAGCCC TAAAAGACTT ATATGAGAGG TGTAATAAAG ATATTATCTG GGCCACAAGC 
CTTTTGTTGG ATTCTGAAAC TAAATTATGT GAAGATACTG AATTTGAGAA TTTCCAGAAA 
TCATATGATG AATCACAAAT TGGGCCATTT TCTCTGGGAT TGAATTTGAA AGAAATTATT 
AGCCACAGGG GAACTTTAGA GGATTCTAAT TCTTCCTTGC CGGAATTTAG CCATGGATTT 
GCTGTCAGTA ACACTAATAC ACAGTCCACC TGTGATGCAG AAAATGAAAA CTCAGAGCAG 
GCAGAAATAA GAGCTATAAC TCCTGAAAAA CCTGATTTAA TAACAAGTAT ATTTCCTAGT 
GCTGCTGTAG ATCTAAAGAA TAATAGTGAA ACACTTCCTA ACAGTCGGGC AGAACTTTTA 
CATAGCATTA AGCAGACTTT TCCAGGTATT TCAAAAGCTA CTACCCCTAA AGATGTGTGT 
GAAATGGAGA AAAATCTAGT AATCACAGAG ACTGGAGACA ATATACATTC TTCTTTAAAT 
CTGTCTATTT TAAACTCTGT GTCAAGCACT TCAAATCTTG AATTAAATGA AGAAATTTAT 
TTTATGGACT CTATTGAAAT AAAGAAAAAC AAAAATCTTC CAAAGGATTA TATGAAATTT 
CCAAACACAG AAGAATTTAT GAATGAAGAT GAGCAGGAAA TGGAGAAAAT TCTAATGGCA 
GGGAGTAGTT TGTCAGCTGG AGTTAGTGAA GAAGATAAAA CTGAAATATT GAATCCCACT 
CCAGTGATGA CCAAATCTCT AACCATAGAC TGTCTGGAAT TGGCATTACC CCCTGAACTG 
GCTTTTCAAC TCAATGAATT ATTTGGCCCT GTTGGTATTG ATTCAGGATC TCTAACAGTT 
GAGGATTGTG TGGTTCATAT AGATCTGAAT CTGGCTAAAG TGATTCATGA GAAATGGAAA 
GAATCTGTGA TGGAGCGACA AAGACAAGAA GAGGTATCTT GTGGCAAGTT TAAGCGAGAT 
TCTTCCCTGG TTGGACATAT TGGTCTTGAT AATCCAGAAC AAAAATCATC TCAGAGAACA 
GGCAAAAAAT TACTGAAGAC TTTAGCAGCA TCTGAAATGC TACCTTTATT GGATCATTGG 
AATACTCAAA CTAAAAAAGT ATCACTAAGA GAAATAATGT CAGAAGAAAT TGCCTTACAG 
GAGAAACATG ATCTGAAAAG GGAGACACTT ATGTTTGAAA AAGATTGTGC CACTAAACTA 
AAGGAGAAGC AGCTCTTTAA GATATTTCCA GCCATTAACC AAAATTTCCT GGTGGACATT 
TTCAAGGACC ACAACTATTC ATTAGAACAC ACAGTACAAT TTCTAAACTG TGTTCTTGAA 
GGGGACCCTG TAAAAACAGT TGTAGCACAA GAGTTTGTTC ACCAAAATGA GAATGTCACT 
TCTCATACTA CCCAGAAGTC TAAAGAGAAA AAGGCAAAGA AATCAAAAGA GTCTGAAGAT 
ACACCAAGCG AACCATCTTT TCAGGATTTC GAGTACCCAG AATATGACGA CTATAGGGCA 
GAGGCTTTCC TGCACCAACA GAAGAGGATA GAATGCTACA GTAAGGCAAA AGAAGCTTAT 
CGGATGGGGA AGAAAAATGT TGCCACCTTT TACGCCCAAC AGGGTAGTCT TCATGAGCAG 
AAGATGAAAG AAGCCAATCA CCTTGCTGCT GTGGAGATCT TTGAGAAAGT CAATGCCTCG 
CTGCTGCCAC AGAATGTTTT AGACCTCCAT GGGCTGCATG TGGATGAAGC TATAGAACAA 
TTGATGACAG TTTTACAGCA GAAAACTGAA GAATTTAAGC AGAATGGTGG TAAGCCCTAT 
CTGTCTGTGA TCACGGGGAG AGGAAACCAC AGCCAGGGAG GAGTTGCTCG CATCAAGCCA 
GCCGTCATTA AATACCTCAC AAGCCATAGC TTCAGGTTCT CTGAAATTAA ACCAGGGTGC 
TTGAAAGTCA TGCTAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609409.1
CDS	1281..6581
Translation	MPRRKKNVGGNPFRKTANSKEVVVSSVTSHEEPTATLPSMGETKVDQEELFTSISEMFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELARRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753955.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753955.2

ORF Insert Sequence:

ATGCCAAGGA GAAAGAAAAA TGTTGGGGGA AATCCTTTTC GGAAGACTGC AAACTCTAAG 
GAAGTTGTCG TATCCAGTGT TACTAGTCAT GAGGAGCCAA CTGCTACTCT TCCTTCCATG 
GGTGAGACAA AAGTTGATCA GGAAGAACTC TTCACCAGTA TCTCAGAGAT GTTTTCTGAT 
CTGGATCCTG ATGTAGTGTA TTTGATGCTT TCTGAATGTG ATTTCAAAGT TGAAAATGCT 
ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGGCG TAACATTCAT 
GGTGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACACT ATCAACGTTT TGTTTCAGTG 
CCAATAATCA TGAGTTCTTC GGTTCCAGAG AAAATTGAAC GTATTGAGTT GTGTGCATAT 
TCTTGTGAGG ATAAAAGTAC TGGCCCAAGA GAAAATGAAG ATATCACCTC TGAAAAAGAA 
GAAAATGTTT TATCCTCATC GTTGAAGCAC CTAGAGTTAA TTGAAGAGAA GAGTCTTGAA 
GTGACCAAAG AAACTATGTT ACCTGAGAAT GTTACGTATC TCCCTGATGC AGATTTAAAC 
AAAGGAAGAA AAGAAATAAG TGGTATGAGG CAAAACATTC AAAGTACTAA CATTCAGGAA 
GTTCCACACA TGTATTTTTC TGACTCTGAA AGCAAAGTAA GAGTGATGGA CAAAAGTGAA 
GAAGAAGTAG AAATGTCATC TGAAAAAGAG TGTGGTAAAA CTGATGCAGA TAGTTTTGTT 
GAGAGAGTAT CACTGAGTGT TTGCTATGAT GAAAATAATC AAGAAAATTG TGATCTTGTA 
AATACTGTAT CCCTTCAAAA CGAAAAATCT TCACCTGGCG AAACATTAGA AGAAAGAGCA 
ACAGTAAAGA AAAAAGCCTT TGGGAAACAA AAAAGTAAAT TGACTTTGGA AAAGTTCCCA 
AGACATGAGC TATCAGATTT TGTTGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG 
ACAAAAAGGA ACCGAAAAAC TGAAAAAACA TCTGTACAAA GTGACAGAAA GTATAATTGC 
CCTCAGTCAT GCAAAGTATT AGATAATAGT GTATCTGTGA ATATAGATTG TATCCAGAAA 
CAAGGATCTC CACACGAAAG TGTAAAGGAT GGCAAGAAGT CACAGGGTGA TGCTGTTTCA 
GAGTCACTCA ATAGCTGTAA ATGCAATCCT TGTAAAAATA CTGAAAAAAA CTCATTCAGC 
ATTGTGGGAG ATTGGCCTTC ATCTGACTCT TTAGCTCCGA GGGAGCACAG ATCAAGAATG 
ACAAAGACTG GTTTAAATGA ACCCAACCTA GAACTTGGAA CAAATGACAA TATGAGTGAA 
ATATTTTTAT ACACAGCACG TGAGGCCTGT TGGGACACAA ACCTTGAAAA ACTAAGAACA 
TTGGGCAGCT CCAGTCTAGA AAATTCCGAA ATGCTACCCA GTGAAATGAG CTGTGAGAGT 
CAGAGTTGTC TAAGTAAAAA GATTCATGGG CAATACACGT TGCCTCTTAC CAATAGCGCA 
CCAACTATTC CTGGAATAGT AGGACCACAA ACTTCAGCTG AATTTCCAGA GGGAATGCCT 
CAAGGAGTCC CTGGAATAGA AGTAGGCATA TGTACTCAGA CTGAACCACA GGATTTTGCT 
CTCTTATGGA AAATAGAAAA GAATAAAATT AGTATTTCAG ATTCTGTCAA AGTATTAACA 
GGAAGATTAG ATGGATTTAA GCCGAAAGTT TTCAATATTA ACACAAACTC AGACGTTCAA 
GAAACAATTC CATATAGGGT GATGTATGAT AAAAGCACAT ATGTTGAAGA AAGTGAGCTT 
ACCAGTGCTG ATGAATCTGA AAATCTTAAC ATTCTTTGTA AGCTATTTGG ATCTGTTTCA 
TTAGAAGCCC TAAAAGACTT ATATGAGAGG TGTAATAAAG ATATTATCTG GGCCACAAGC 
CTTTTGTTGG ATTCTGAAAC TAAATTATGT GAAGATACTG AATTTGAGAA TTTCCAGAAA 
TCATATGATG AATCACAAAT TGGGCCATTT TCTCTGGGAT TGAATTTGAA AGAAATTATT 
AGCCACAGGG GAACTTTAGA GGATTCTAAT TCTTCCTTGC CGGAATTTAG CCATGGATTT 
GCTGTCAGTA ACACTAATAC ACAGTCCACC TGTGATGCAG AAAATGAAAA CTCAGAGCAG 
GCAGAAATAA GAGCTATAAC TCCTGAAAAA CCTGATTTAA TAACAAGTAT ATTTCCTAGT 
GCTGCTGTAG ATCTAAAGAA TAATAGTGAA ACACTTCCTA ACAGTCGGGC AGAACTTTTA 
CATAGCATTA AGCAGACTTT TCCAGGTATT TCAAAAGCTA CTACCCCTAA AGATGTGTGT 
GAAATGGAGA AAAATCTAGT AATCACAGAG ACTGGAGACA ATATACATTC TTCTTTAAAT 
CTGTCTATTT TAAACTCTGT GTCAAGCACT TCAAATCTTG AATTAAATGA AGAAATTTAT 
TTTATGGACT CTATTGAAAT AAAGAAAAAC AAAAATCTTC CAAAGGATTA TATGAAATTT 
CCAAACACAG AAGAATTTAT GAATGAAGAT GAGCAGGAAA TGGAGAAAAT TCTAATGGCA 
GGGAGTAGTT TGTCAGCTGG AGTTAGTGAA GAAGATAAAA CTGAAATATT GAATCCCACT 
CCAGTGATGA CCAAATCTCT AACCATAGAC TGTCTGGAAT TGGCATTACC CCCTGAACTG 
GCTTTTCAAC TCAATGAATT ATTTGGCCCT GTTGGTATTG ATTCAGGATC TCTAACAGTT 
GAGGATTGTG TGGTTCATAT AGATCTGAAT CTGGCTAAAG TGATTCATGA GAAATGGAAA 
GAATCTGTGA TGGAGCGACA AAGACAAGAA GAGGTATCTT GTGGCAAGTT TAAGCGAGAT 
TCTTCCCTGG TTGGACATAT TGGTCTTGAT AATCCAGAAC AAAAATCATC TCAGAGAACA 
GGCAAAAAAT TACTGAAGAC TTTAGCAGCA TCTGAAATGC TACCTTTATT GGATCATTGG 
AATACTCAAA CTAAAAAAGT ATCACTAAGA GAAATAATGT CAGAAGAAAT TGCCTTACAG 
GAGAAACATG ATCTGAAAAG GGAGACACTT ATGTTTGAAA AAGATTGTGC CACTAAACTA 
AAGGAGAAGC AGCTCTTTAA GATATTTCCA GCCATTAACC AAAATTTCCT GGTGGACATT 
TTCAAGGACC ACAACTATTC ATTAGAACAC ACAGTACAAT TTCTAAACTG TGTTCTTGAA 
GGGGACCCTG TAAAAACAGT TGTAGCACAA GAGTTTGTTC ACCAAAATGA GAATGTCACT 
TCTCATACTA CCCAGAAGTC TAAAGAGAAA AAGGCAAAGA AATCAAAAGA GTCTGAAGAT 
ACACCAAGCG AACCATCTTT TCAGGATTTC GAGTACCCAG AATATGACGA CTATAGGGCA 
GAGGCTTTCC TGCACCAACA GAAGAGGATA GAATGCTACA GTAAGGCAAA AGAAGCTTAT 
CGGATGGGGA AGAAAAATGT TGCCACCTTT TACGCCCAAC AGGGTAGTCT TCATGAGCAG 
AAGATGAAAG AAGCCAATCA CCTTGCTGCT GTGGAGATCT TTGAGAAAGT CAATGCCTCG 
CTGCTGCCAC AGAATGTTTT AGACCTCCAT GGGCTGCATG TGGATGAAGC TATAGAACAA 
TTGATGACAG TTTTACAGCA GAAAACTGAA GAATTTAAGC AGAATGGTGG TAAGCCCTAT 
CTGTCTGTGA TCACGGGGAG AGGAAACCAC AGCCAGGGAG GAGTTGCTCG CATCAAGCCA 
GCCGTCATTA AATACCTCAC AAGCCATAGC TTCAGGTTCT CTGAAATTAA ACCAGGGTGC 
TTGAAAGTCA TGCTAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753961.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609415.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X31, mRNA.

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CloneID	OMi70583
Clone ID Related Accession (Same CDS sequence)	XM_012753961.2 , XM_012753965.2 , XM_012753956.2 , XM_012753958.2 , XM_012753959.1 , XM_012753963.2 , XM_012753964.1 , XM_012753957.2 , XM_012753960.1 , XM_012753966.2
Accession Version	XM_012753961.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609415.1
CDS	385..5460
Translation	MDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753961.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753961.2

ORF Insert Sequence:

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753965.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609419.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X34, mRNA.

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CloneID	OMi70583
Clone ID Related Accession (Same CDS sequence)	XM_012753961.2 , XM_012753965.2 , XM_012753956.2 , XM_012753958.2 , XM_012753959.1 , XM_012753963.2 , XM_012753964.1 , XM_012753957.2 , XM_012753960.1 , XM_012753966.2
Accession Version	XM_012753965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609419.1
CDS	338..5413
Translation	MDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753965.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753965.2

ORF Insert Sequence:

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753956.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609410.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X26, mRNA.

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CloneID	OMi70583
Clone ID Related Accession (Same CDS sequence)	XM_012753961.2 , XM_012753965.2 , XM_012753956.2 , XM_012753958.2 , XM_012753959.1 , XM_012753963.2 , XM_012753964.1 , XM_012753957.2 , XM_012753960.1 , XM_012753966.2
Accession Version	XM_012753956.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609410.1
CDS	1270..6345
Translation	MDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753956.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753956.2

ORF Insert Sequence:

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753958.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609412.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OMi70583
Clone ID Related Accession (Same CDS sequence)	XM_012753961.2 , XM_012753965.2 , XM_012753956.2 , XM_012753958.2 , XM_012753959.1 , XM_012753963.2 , XM_012753964.1 , XM_012753957.2 , XM_012753960.1 , XM_012753966.2
Accession Version	XM_012753958.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609412.1
CDS	1172..6247
Translation	MDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753958.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753958.2

ORF Insert Sequence:

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753959.1 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609413.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OMi70583
Clone ID Related Accession (Same CDS sequence)	XM_012753961.2 , XM_012753965.2 , XM_012753956.2 , XM_012753958.2 , XM_012753959.1 , XM_012753963.2 , XM_012753964.1 , XM_012753957.2 , XM_012753960.1 , XM_012753966.2
Accession Version	XM_012753959.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609413.1
CDS	193..5268
Translation	MDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753959.1
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753959.1

ORF Insert Sequence:

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753963.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609417.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X32, mRNA.

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CloneID	OMi70583
Clone ID Related Accession (Same CDS sequence)	XM_012753961.2 , XM_012753965.2 , XM_012753956.2 , XM_012753958.2 , XM_012753959.1 , XM_012753963.2 , XM_012753964.1 , XM_012753957.2 , XM_012753960.1 , XM_012753966.2
Accession Version	XM_012753963.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753963.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609417.1
CDS	1080..6155
Translation	MDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753963.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753963.2

ORF Insert Sequence:

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753964.1 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609418.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X33, mRNA.

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CloneID	OMi70583
Clone ID Related Accession (Same CDS sequence)	XM_012753961.2 , XM_012753965.2 , XM_012753956.2 , XM_012753958.2 , XM_012753959.1 , XM_012753963.2 , XM_012753964.1 , XM_012753957.2 , XM_012753960.1 , XM_012753966.2
Accession Version	XM_012753964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609418.1
CDS	164..5239
Translation	MDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753964.1
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753964.1

ORF Insert Sequence:

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753957.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609411.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X27, mRNA.

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CloneID	OMi70583
Clone ID Related Accession (Same CDS sequence)	XM_012753961.2 , XM_012753965.2 , XM_012753956.2 , XM_012753958.2 , XM_012753959.1 , XM_012753963.2 , XM_012753964.1 , XM_012753957.2 , XM_012753960.1 , XM_012753966.2
Accession Version	XM_012753957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609411.1
CDS	1178..6253
Translation	MDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753957.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753957.2

ORF Insert Sequence:

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753960.1 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609414.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X30, mRNA.

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CloneID	OMi70583
Clone ID Related Accession (Same CDS sequence)	XM_012753961.2 , XM_012753965.2 , XM_012753956.2 , XM_012753958.2 , XM_012753959.1 , XM_012753963.2 , XM_012753964.1 , XM_012753957.2 , XM_012753960.1 , XM_012753966.2
Accession Version	XM_012753960.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609414.1
CDS	256..5331
Translation	MDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753960.1
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753960.1

ORF Insert Sequence:

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N4BP2 ( XM_012753966.2 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_012609420.1 Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X35, mRNA.

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CloneID	OMi70583
Clone ID Related Accession (Same CDS sequence)	XM_012753961.2 , XM_012753965.2 , XM_012753956.2 , XM_012753958.2 , XM_012753959.1 , XM_012753963.2 , XM_012753964.1 , XM_012753957.2 , XM_012753960.1 , XM_012753966.2
Accession Version	XM_012753966.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	NEDD4-binding protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 24, 2017 this sequence version replaced XM_012753966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012609420.1
CDS	86..5161
Translation	MDCLLELSATDAKIEESSSQSFIASENQVSAAESEVMEKCPPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQVYSFLPLQDVSSFNDPSEFINSDLSTMTPILSTHSMNLNSENLENSASTLSSNAFPSDLVFNESKHFIKDNMLALEGKYPEDSLLSSSLNPANDSIVGCSNLDQKQKELLESECAEAQFPQASVHLDASEPQAPLSLPGLDLPGTGGDQKSTFVSDVFVPSEGFNFKPHRHPELPPKGKDVSYCPVLTPLPLLLPPPPPPVWSPGVPALDLFHGSRGFVAPVVTTAAHWRPVNYTFPPSVISHTSPTKVWRNKEGTSAYQVQETPVSQVVRKKTTSYVGLVLVLLRGLPGSGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAREAFEKKITPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPETWWKFKPKELASQTRLRRNIHGVSKEKITRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDKSTGPRENEDITSEKEENVLSSSLKHLELIEEKSLEVTKETMLPENVTYLPDADLNKGRKEISGMRQNIQSTNIQEVPHMYFSDSESKVRVMDKSEEEVEMSSEKECGKTDADSFVERVSLSVCYDENNQENCDLVNTVSLQNEKSSPGETLEERATVKKKAFGKQKSKLTLEKFPRHELSDFVGDWPVDKTIGQRTKRNRKTEKTSVQSDRKYNCPQSCKVLDNSVSVNIDCIQKQGSPHESVKDGKKSQGDAVSESLNSCKCNPCKNTEKNSFSIVGDWPSSDSLAPREHRSRMTKTGLNEPNLELGTNDNMSEIFLYTAREACWDTNLEKLRTLGSSSLENSEMLPSEMSCESQSCLSKKIHGQYTLPLTNSAPTIPGIVGPQTSAEFPEGMPQGVPGIEVGICTQTEPQDFALLWKIEKNKISISDSVKVLTGRLDGFKPKVFNINTNSDVQETIPYRVMYDKSTYVEESELTSADESENLNILCKLFGSVSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSYDESQIGPFSLGLNLKEIISHRGTLEDSNSSLPEFSHGFAVSNTNTQSTCDAENENSEQAEIRAITPEKPDLITSIFPSAAVDLKNNSETLPNSRAELLHSIKQTFPGISKATTPKDVCEMEKNLVITETGDNIHSSLNLSILNSVSSTSNLELNEEIYFMDSIEIKKNKNLPKDYMKFPNTEEFMNEDEQEMEKILMAGSSLSAGVSEEDKTEILNPTPVMTKSLTIDCLELALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEVSCGKFKRDSSLVGHIGLDNPEQKSSQRTGKKLLKTLAASEMLPLLDHWNTQTKKVSLREIMSEEIALQEKHDLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSLEHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTTQKSKEKKAKKSKESEDTPSEPSFQDFEYPEYDDYRAEAFLHQQKRIECYSKAKEAYRMGKKNVATFYAQQGSLHEQKMKEANHLAAVEIFEKVNASLLPQNVLDLHGLHVDEAIEQLMTVLQQKTEEFKQNGGKPYLSVITGRGNHSQGGVARIKPAVIKYLTSHSFRFSEIKPGCLKVMLK

Target ORF information:

RefSeq Version	XM_012753966.2
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus NEDD4 binding protein 2 (N4BP2), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012753966.2

ORF Insert Sequence:

ATGGATTGTC TGTTAGAGTT ATCTGCCACT GATGCCAAGA TAGAAGAATC GTCTTCACAA 
AGTTTCATTG CTTCTGAGAA CCAAGTAAGT GCAGCAGAAA GTGAAGTAAT GGAAAAGTGT 
CCTCCTGAAG AAGAGAGTGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT 
GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TAAACTCTTC TCCTGATGAC 
CAAGTATACT CATTTTTGCC TTTGCAAGAT GTTAGTAGTT TTAATGACCC GAGTGAGTTT 
ATAAATTCTG ATTTAAGTAC TATGACTCCC ATTCTTTCTA CACACAGTAT GAATTTGAAC 
AGTGAAAATT TAGAGAATTC TGCCTCCACA TTAAGTTCAA ACGCATTTCC TTCAGATTTG 
GTTTTCAATG AGTCCAAACA TTTTATAAAG GATAACATGT TGGCATTGGA AGGTAAATAC 
CCAGAAGATT CTCTTCTCAG TAGTTCTTTA AATCCAGCAA ATGACTCTAT TGTAGGTTGT 
AGCAATCTCG ATCAAAAACA GAAAGAACTT TTAGAATCTG AGTGTGCTGA GGCTCAGTTC 
CCTCAAGCTT CTGTGCATCT GGATGCCAGT GAACCTCAGG CACCTTTAAG CCTTCCAGGG 
CTTGATTTAC CAGGTACAGG TGGCGATCAG AAATCGACTT TTGTCTCTGA TGTGTTTGTA 
CCTTCTGAAG GGTTCAATTT TAAGCCACAC AGGCATCCTG AACTGCCACC AAAGGGGAAG 
GATGTGAGTT ACTGCCCGGT ACTCACTCCC CTCCCTTTGC TGCTGCCGCC TCCCCCTCCG 
CCAGTGTGGA GCCCAGGGGT TCCTGCTTTG GACCTCTTTC ATGGGAGCCG TGGCTTTGTA 
GCTCCTGTTG TGACCACAGC TGCACACTGG AGACCTGTCA ACTATACGTT TCCACCCTCA 
GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGGAACA AAGAAGGAAC AAGTGCTTAT 
CAAGTACAAG AAACCCCCGT TTCTCAGGTT GTAAGAAAGA AGACAACATC TTACGTAGGA 
CTAGTTCTTG TTCTTCTCAG AGGCCTTCCT GGATCAGGAA AATCTTTTCT GGCAAGGACT 
TTGCAAGAGG ATAATCCGAG TGGAGTCATT CTTAGTACTG ACGATTATTT TTACATAAAT 
GGACAGTATC AGTTTGATGT AAAGTACTTG GGAGAAGCAC ATGAATGGAA TCAGAATCGT 
GCAAGAGAAG CATTTGAGAA GAAGATAACT CCTATAATAA TAGACAATAC AAATCTACAG 
GCATGGGAAA TGAAGCCATA TGTTGCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC 
CGGGAACCAG AAACATGGTG GAAGTTCAAA CCAAAGGAAC TTGCAAGCCA GACAAGATTG 
AGGCGTAACA TTCATGGTGT AAGCAAAGAA AAAATAACAA GAATGTTGGA ACACTATCAA 
CGTTTTGTTT CAGTGCCAAT AATCATGAGT TCTTCGGTTC CAGAGAAAAT TGAACGTATT 
GAGTTGTGTG CATATTCTTG TGAGGATAAA AGTACTGGCC CAAGAGAAAA TGAAGATATC 
ACCTCTGAAA AAGAAGAAAA TGTTTTATCC TCATCGTTGA AGCACCTAGA GTTAATTGAA 
GAGAAGAGTC TTGAAGTGAC CAAAGAAACT ATGTTACCTG AGAATGTTAC GTATCTCCCT 
GATGCAGATT TAAACAAAGG AAGAAAAGAA ATAAGTGGTA TGAGGCAAAA CATTCAAAGT 
ACTAACATTC AGGAAGTTCC ACACATGTAT TTTTCTGACT CTGAAAGCAA AGTAAGAGTG 
ATGGACAAAA GTGAAGAAGA AGTAGAAATG TCATCTGAAA AAGAGTGTGG TAAAACTGAT 
GCAGATAGTT TTGTTGAGAG AGTATCACTG AGTGTTTGCT ATGATGAAAA TAATCAAGAA 
AATTGTGATC TTGTAAATAC TGTATCCCTT CAAAACGAAA AATCTTCACC TGGCGAAACA 
TTAGAAGAAA GAGCAACAGT AAAGAAAAAA GCCTTTGGGA AACAAAAAAG TAAATTGACT 
TTGGAAAAGT TCCCAAGACA TGAGCTATCA GATTTTGTTG GTGACTGGCC AGTTGATAAG 
ACTATTGGTC AGAGGACAAA AAGGAACCGA AAAACTGAAA AAACATCTGT ACAAAGTGAC 
AGAAAGTATA ATTGCCCTCA GTCATGCAAA GTATTAGATA ATAGTGTATC TGTGAATATA 
GATTGTATCC AGAAACAAGG ATCTCCACAC GAAAGTGTAA AGGATGGCAA GAAGTCACAG 
GGTGATGCTG TTTCAGAGTC ACTCAATAGC TGTAAATGCA ATCCTTGTAA AAATACTGAA 
AAAAACTCAT TCAGCATTGT GGGAGATTGG CCTTCATCTG ACTCTTTAGC TCCGAGGGAG 
CACAGATCAA GAATGACAAA GACTGGTTTA AATGAACCCA ACCTAGAACT TGGAACAAAT 
GACAATATGA GTGAAATATT TTTATACACA GCACGTGAGG CCTGTTGGGA CACAAACCTT 
GAAAAACTAA GAACATTGGG CAGCTCCAGT CTAGAAAATT CCGAAATGCT ACCCAGTGAA 
ATGAGCTGTG AGAGTCAGAG TTGTCTAAGT AAAAAGATTC ATGGGCAATA CACGTTGCCT 
CTTACCAATA GCGCACCAAC TATTCCTGGA ATAGTAGGAC CACAAACTTC AGCTGAATTT 
CCAGAGGGAA TGCCTCAAGG AGTCCCTGGA ATAGAAGTAG GCATATGTAC TCAGACTGAA 
CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAGTAT TTCAGATTCT 
GTCAAAGTAT TAACAGGAAG ATTAGATGGA TTTAAGCCGA AAGTTTTCAA TATTAACACA 
AACTCAGACG TTCAAGAAAC AATTCCATAT AGGGTGATGT ATGATAAAAG CACATATGTT 
GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAACATTCT TTGTAAGCTA 
TTTGGATCTG TTTCATTAGA AGCCCTAAAA GACTTATATG AGAGGTGTAA TAAAGATATT 
ATCTGGGCCA CAAGCCTTTT GTTGGATTCT GAAACTAAAT TATGTGAAGA TACTGAATTT 
GAGAATTTCC AGAAATCATA TGATGAATCA CAAATTGGGC CATTTTCTCT GGGATTGAAT 
TTGAAAGAAA TTATTAGCCA CAGGGGAACT TTAGAGGATT CTAATTCTTC CTTGCCGGAA 
TTTAGCCATG GATTTGCTGT CAGTAACACT AATACACAGT CCACCTGTGA TGCAGAAAAT 
GAAAACTCAG AGCAGGCAGA AATAAGAGCT ATAACTCCTG AAAAACCTGA TTTAATAACA 
AGTATATTTC CTAGTGCTGC TGTAGATCTA AAGAATAATA GTGAAACACT TCCTAACAGT 
CGGGCAGAAC TTTTACATAG CATTAAGCAG ACTTTTCCAG GTATTTCAAA AGCTACTACC 
CCTAAAGATG TGTGTGAAAT GGAGAAAAAT CTAGTAATCA CAGAGACTGG AGACAATATA 
CATTCTTCTT TAAATCTGTC TATTTTAAAC TCTGTGTCAA GCACTTCAAA TCTTGAATTA 
AATGAAGAAA TTTATTTTAT GGACTCTATT GAAATAAAGA AAAACAAAAA TCTTCCAAAG 
GATTATATGA AATTTCCAAA CACAGAAGAA TTTATGAATG AAGATGAGCA GGAAATGGAG 
AAAATTCTAA TGGCAGGGAG TAGTTTGTCA GCTGGAGTTA GTGAAGAAGA TAAAACTGAA 
ATATTGAATC CCACTCCAGT GATGACCAAA TCTCTAACCA TAGACTGTCT GGAATTGGCA 
TTACCCCCTG AACTGGCTTT TCAACTCAAT GAATTATTTG GCCCTGTTGG TATTGATTCA 
GGATCTCTAA CAGTTGAGGA TTGTGTGGTT CATATAGATC TGAATCTGGC TAAAGTGATT 
CATGAGAAAT GGAAAGAATC TGTGATGGAG CGACAAAGAC AAGAAGAGGT ATCTTGTGGC 
AAGTTTAAGC GAGATTCTTC CCTGGTTGGA CATATTGGTC TTGATAATCC AGAACAAAAA 
TCATCTCAGA GAACAGGCAA AAAATTACTG AAGACTTTAG CAGCATCTGA AATGCTACCT 
TTATTGGATC ATTGGAATAC TCAAACTAAA AAAGTATCAC TAAGAGAAAT AATGTCAGAA 
GAAATTGCCT TACAGGAGAA ACATGATCTG AAAAGGGAGA CACTTATGTT TGAAAAAGAT 
TGTGCCACTA AACTAAAGGA GAAGCAGCTC TTTAAGATAT TTCCAGCCAT TAACCAAAAT 
TTCCTGGTGG ACATTTTCAA GGACCACAAC TATTCATTAG AACACACAGT ACAATTTCTA 
AACTGTGTTC TTGAAGGGGA CCCTGTAAAA ACAGTTGTAG CACAAGAGTT TGTTCACCAA 
AATGAGAATG TCACTTCTCA TACTACCCAG AAGTCTAAAG AGAAAAAGGC AAAGAAATCA 
AAAGAGTCTG AAGATACACC AAGCGAACCA TCTTTTCAGG ATTTCGAGTA CCCAGAATAT 
GACGACTATA GGGCAGAGGC TTTCCTGCAC CAACAGAAGA GGATAGAATG CTACAGTAAG 
GCAAAAGAAG CTTATCGGAT GGGGAAGAAA AATGTTGCCA CCTTTTACGC CCAACAGGGT 
AGTCTTCATG AGCAGAAGAT GAAAGAAGCC AATCACCTTG CTGCTGTGGA GATCTTTGAG 
AAAGTCAATG CCTCGCTGCT GCCACAGAAT GTTTTAGACC TCCATGGGCT GCATGTGGAT 
GAAGCTATAG AACAATTGAT GACAGTTTTA CAGCAGAAAA CTGAAGAATT TAAGCAGAAT 
GGTGGTAAGC CCTATCTGTC TGTGATCACG GGGAGAGGAA ACCACAGCCA GGGAGGAGTT 
GCTCGCATCA AGCCAGCCGT CATTAAATAC CTCACAAGCC ATAGCTTCAG GTTCTCTGAA 
ATTAAACCAG GGTGCTTGAA AGTCATGCTA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	N4BP2
Entrez Gene ID	105865415
Full Name	NEDD4 binding protein 2
Gene Type	protein-coding
Organism	Microcebus murinus(gray mouse lemur)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

N4BP2 cDNA ORF clone, Microcebus murinus(gray mouse lemur)

Gene

Clones

gRNAs

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