LOC105661748 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105661748
Entrez Gene ID	105661748
Full Name	uncharacterized LOC105661748
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012280604.1	XP_012135994.1	uncharacterized protein LOC105661748

Pathways from BioSystems
Gene Ontology

The following LOC105661748 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105661748 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe100527	XM_012280604.1 Latest version!	Megachile rotundata uncharacterized LOC105661748 (LOC105661748), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105661748 ( XM_012280604.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012135994.1 Megachile rotundata uncharacterized LOC105661748 (LOC105661748), mRNA.

Price & Availability↑

CloneID	OMe100527
Clone ID Related Accession (Same CDS sequence)	XM_012280604.1
Accession Version	XM_012280604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	uncharacterized protein LOC105661748
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797109.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGGATAACC CGAAACAAAT GTTGCAACGA CAAAATTGTA TTTCTAACGG TAAGGAGGAC 
GATGACGTGA GCGTGATTGC TATAATAGAA AAAGAAGACG ATCGTTCATC GCGACAGCAA 
TCCGAAAGAC CCAGTGAGAA ACAAGAACGA CCGGAGGAAA CTGTCGCGGA GGAATATAGG 
AACTTGGAAC TTTTACCGAA ACTGCCTCTC GGTAGGTTTA ATATCAAGCA GGATAAAAAG 
TATAAAGACG ACAGTGATTA CGTGCGTGAT TCCACGGAAA CGAGCAGTGG CGGTTCATCT 
TCCGGTAGTG GCGTTGCTAA AGTGAGGAGA GTTCGAACCG TAAGGAAAAC CCGCAGCGCA 
GCAGAAGTAA GAAGAAATCC AGTACGCGCG AAGAAGAAGG ACCCCGACAA AAATTTCCCG 
CGATTAAGAC AGCGGTTACT TTCCGCGAAA AGGAAAGAGA TGCTTTTCGA CCCAGAGCCC 
AGTAAACGAG GCAAATCTTC CACAAAGCCG TACATGAACA CGAGGTCCGT CACGAGAAAA 
ATGTACAACG TAGGAGCGAC TTATCAGGCG CCCACCATGA AGGATGCTCT GGAATGGAAG 
GAATGGCCTG TGCATGGAAT GCACGAGAGA CCAGTCTTCC ATCCACAGCT TGGTCTGGCT 
GCTGAATATT TGGGACGGTA TTTTACGAGC TTCGACGGAC TTTCCTATTG CGAAATCATA 
GATCGGCCGG AAGTGGAGGT AGTGTCTGTC GATCCCCTAC GCGATTCTCT TCCTTCGTTC 
TACGAGAAAA GAAGAGGCAA AATTAAGACC AAAAGCTCCA CGAATGTTAA ACCAAAGAAT 
TCTTCAGAAA AGAAAAAATC GTTTGAAAAT TGTATGCACG AAAGTTTTCA TTGTGTTCTC 
GGTTACTGCT CGCAGGTAAT GACTCCTACT TATAAGAACA CCGTGGAGGA AAAGCTGGAT 
ATGCTGAATG AAAACAAAAC GTGTACTATC GTCAAAGAAA CACAGCAGCC TGTAAACGCT 
GTTGAAAACA GTCCTATAAG CAAGTCACCA AATAATTCTA AAGAGAATAT ACAGAAATCT 
CAAGAAAGTA AAGCTGCGGA TAGTTACACA GCAGTTGTGA TACCACAGAA CGTTAAGAAC 
TTCAATCAGA TTCCAAAAAC GCAATTGTTT CCAGCGCACA AAGTCTACCT TGCAAATACA 
CAAAAGTCTA TCGCCTTCAG TAACGTGAGA ACGCTTCAGG GAGGACAAAT GAAGATTCAA 
GAGGTTATAC GACCTAGCAA GAATCCTTTC ATATTAGTTA ATACAGATAC CAAGGATACG 
CGGTTTCTAA AATCTACATC AAGCAACGTG AACGTAAAAC CAGCGGATAC ACCGGTTCTT 
GAAGAAAATT TAGGCGAGTT ATCATCGATA TACAAACACT TCGACCTCAG CGAACAGATG 
TCCTCGAAGA AGACTCCACC GAAACGCGAG AAGAAGGTAG AATTCGCCAT GACCAGATAC 
TTGCTGAACA CCGCAAAGCA AACCAAGATT CAGATACCAA ATGTTGGCGA AGGGAATTTG 
TATTGCGAGG TGCCTAAAAA GGGTCAAGGA CAAGGTGTGA ACAAGACTTG TTACACCAAC 
GAAGCTTCAG AGATTGCAAA GATCCTCTCT GAGTACAACA AGAACACTGC TAAAAAGTCA 
ACCCCAGAGA ACACCCCTTT CTGTTCTGTG AAGAATATCA GAGTCAATGA ACTTTGCAAC 
AAAGACGAGA ACACGAACCA AAACTCCCCG GAAAGAAATA GAAATATTTC GTTTCCCCAG 
GGAAAGTGGA GGCGGTTCCA CTTGACTATG GGAAAGCCAA AGGACTTGAA AAGTAATACG 
ACTGATCAAA GATTTTTATC GGGTATTCAA AACAGACAGT CGCTCTTCAG AATCAATCCG 
ACAGGCTTGG ATTCCACGCC TGGGAGAATG ATCGAAGTGT CGAAGCACGT GGAGACGCTT 
CAGCGAAACC AGACTACAAC GAATGCTGAG AAGAAAGAGA GCACGTCTAC GGTGAATGAC 
GATGCTGATT CGACGGGGAC CAAGGCACAG TACCTGCAGG ACTTGCTTGA AGACACAGCC 
ATGTTGTACT GTGCAGCCAC TGGCACACAT CAGGACGATC TAGCTACTTA TATTGACACT 
TTGGATGCTG TGCAGAGTGT GCAATGGTTG GACACTTGCA AGAATCCGAG TAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012135994.1
CDS	259..2475
Translation	MDNPKQMLQRQNCISNGKEDDDVSVIAIIEKEDDRSSRQQSERPSEKQERPEETVAEEYRNLELLPKLPLGRFNIKQDKKYKDDSDYVRDSTETSSGGSSSGSGVAKVRRVRTVRKTRSAAEVRRNPVRAKKKDPDKNFPRLRQRLLSAKRKEMLFDPEPSKRGKSSTKPYMNTRSVTRKMYNVGATYQAPTMKDALEWKEWPVHGMHERPVFHPQLGLAAEYLGRYFTSFDGLSYCEIIDRPEVEVVSVDPLRDSLPSFYEKRRGKIKTKSSTNVKPKNSSEKKKSFENCMHESFHCVLGYCSQVMTPTYKNTVEEKLDMLNENKTCTIVKETQQPVNAVENSPISKSPNNSKENIQKSQESKAADSYTAVVIPQNVKNFNQIPKTQLFPAHKVYLANTQKSIAFSNVRTLQGGQMKIQEVIRPSKNPFILVNTDTKDTRFLKSTSSNVNVKPADTPVLEENLGELSSIYKHFDLSEQMSSKKTPPKREKKVEFAMTRYLLNTAKQTKIQIPNVGEGNLYCEVPKKGQGQGVNKTCYTNEASEIAKILSEYNKNTAKKSTPENTPFCSVKNIRVNELCNKDENTNQNSPERNRNISFPQGKWRRFHLTMGKPKDLKSNTTDQRFLSGIQNRQSLFRINPTGLDSTPGRMIEVSKHVETLQRNQTTTNAEKKESTSTVNDDADSTGTKAQYLQDLLEDTAMLYCAATGTHQDDLATYIDTLDAVQSVQWLDTCKNPSR

Target ORF information:

RefSeq Version	XM_012280604.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata uncharacterized LOC105661748 (LOC105661748), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012280604.1

ORF Insert Sequence:

ATGGATAACC CGAAACAAAT GTTGCAACGA CAAAATTGTA TTTCTAACGG TAAGGAGGAC 
GATGACGTGA GCGTGATTGC TATAATAGAA AAAGAAGACG ATCGTTCATC GCGACAGCAA 
TCCGAAAGAC CCAGTGAGAA ACAAGAACGA CCGGAGGAAA CTGTCGCGGA GGAATATAGG 
AACTTGGAAC TTTTACCGAA ACTGCCTCTC GGTAGGTTTA ATATCAAGCA GGATAAAAAG 
TATAAAGACG ACAGTGATTA CGTGCGTGAT TCCACGGAAA CGAGCAGTGG CGGTTCATCT 
TCCGGTAGTG GCGTTGCTAA AGTGAGGAGA GTTCGAACCG TAAGGAAAAC CCGCAGCGCA 
GCAGAAGTAA GAAGAAATCC AGTACGCGCG AAGAAGAAGG ACCCCGACAA AAATTTCCCG 
CGATTAAGAC AGCGGTTACT TTCCGCGAAA AGGAAAGAGA TGCTTTTCGA CCCAGAGCCC 
AGTAAACGAG GCAAATCTTC CACAAAGCCG TACATGAACA CGAGGTCCGT CACGAGAAAA 
ATGTACAACG TAGGAGCGAC TTATCAGGCG CCCACCATGA AGGATGCTCT GGAATGGAAG 
GAATGGCCTG TGCATGGAAT GCACGAGAGA CCAGTCTTCC ATCCACAGCT TGGTCTGGCT 
GCTGAATATT TGGGACGGTA TTTTACGAGC TTCGACGGAC TTTCCTATTG CGAAATCATA 
GATCGGCCGG AAGTGGAGGT AGTGTCTGTC GATCCCCTAC GCGATTCTCT TCCTTCGTTC 
TACGAGAAAA GAAGAGGCAA AATTAAGACC AAAAGCTCCA CGAATGTTAA ACCAAAGAAT 
TCTTCAGAAA AGAAAAAATC GTTTGAAAAT TGTATGCACG AAAGTTTTCA TTGTGTTCTC 
GGTTACTGCT CGCAGGTAAT GACTCCTACT TATAAGAACA CCGTGGAGGA AAAGCTGGAT 
ATGCTGAATG AAAACAAAAC GTGTACTATC GTCAAAGAAA CACAGCAGCC TGTAAACGCT 
GTTGAAAACA GTCCTATAAG CAAGTCACCA AATAATTCTA AAGAGAATAT ACAGAAATCT 
CAAGAAAGTA AAGCTGCGGA TAGTTACACA GCAGTTGTGA TACCACAGAA CGTTAAGAAC 
TTCAATCAGA TTCCAAAAAC GCAATTGTTT CCAGCGCACA AAGTCTACCT TGCAAATACA 
CAAAAGTCTA TCGCCTTCAG TAACGTGAGA ACGCTTCAGG GAGGACAAAT GAAGATTCAA 
GAGGTTATAC GACCTAGCAA GAATCCTTTC ATATTAGTTA ATACAGATAC CAAGGATACG 
CGGTTTCTAA AATCTACATC AAGCAACGTG AACGTAAAAC CAGCGGATAC ACCGGTTCTT 
GAAGAAAATT TAGGCGAGTT ATCATCGATA TACAAACACT TCGACCTCAG CGAACAGATG 
TCCTCGAAGA AGACTCCACC GAAACGCGAG AAGAAGGTAG AATTCGCCAT GACCAGATAC 
TTGCTGAACA CCGCAAAGCA AACCAAGATT CAGATACCAA ATGTTGGCGA AGGGAATTTG 
TATTGCGAGG TGCCTAAAAA GGGTCAAGGA CAAGGTGTGA ACAAGACTTG TTACACCAAC 
GAAGCTTCAG AGATTGCAAA GATCCTCTCT GAGTACAACA AGAACACTGC TAAAAAGTCA 
ACCCCAGAGA ACACCCCTTT CTGTTCTGTG AAGAATATCA GAGTCAATGA ACTTTGCAAC 
AAAGACGAGA ACACGAACCA AAACTCCCCG GAAAGAAATA GAAATATTTC GTTTCCCCAG 
GGAAAGTGGA GGCGGTTCCA CTTGACTATG GGAAAGCCAA AGGACTTGAA AAGTAATACG 
ACTGATCAAA GATTTTTATC GGGTATTCAA AACAGACAGT CGCTCTTCAG AATCAATCCG 
ACAGGCTTGG ATTCCACGCC TGGGAGAATG ATCGAAGTGT CGAAGCACGT GGAGACGCTT 
CAGCGAAACC AGACTACAAC GAATGCTGAG AAGAAAGAGA GCACGTCTAC GGTGAATGAC 
GATGCTGATT CGACGGGGAC CAAGGCACAG TACCTGCAGG ACTTGCTTGA AGACACAGCC 
ATGTTGTACT GTGCAGCCAC TGGCACACAT CAGGACGATC TAGCTACTTA TATTGACACT 
TTGGATGCTG TGCAGAGTGT GCAATGGTTG GACACTTGCA AGAATCCGAG TAGATAA