LOC100882084 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100882084
Entrez Gene ID	100882084
Full Name	transforming growth factor beta regulator 1
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003705544.2	XP_003705592.1	transforming growth factor beta regulator 1

Pathways from BioSystems
Gene Ontology

The following LOC100882084 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100882084 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe109308	XM_003705544.2 Latest version!	Megachile rotundata transforming growth factor beta regulator 1 (LOC100882084), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100882084 ( XM_003705544.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003705592.1 Megachile rotundata transforming growth factor beta regulator 1 (LOC100882084), mRNA.

Price & Availability↑

CloneID	OMe109308
Clone ID Related Accession (Same CDS sequence)	XM_003705544.2
Accession Version	XM_003705544.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	transforming growth factor beta regulator 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797307.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003705544.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACAGG CTTATGATAA TTATTATAAT GATTTTCAAA AGAATGCCGA ATTACAACAA 
AATCTGAAAT ATAAAAAGAA ATATCGAAGA TTAAAGCGAA TCATTAAGGA TACAGTATTC 
GAAAATGCAG CTCTTTGTGA TCAAGTAGCA CAAATGCAAG AAAATCTTAT GTTAGTGAAA 
GAGGAAAGAT TATTCCTTTT ACGTAAATTA TGTCAACAAC AAGGAGATAT AGATCCTTCC 
TCTATGGTAG CTCGATCTCA GTCAAATAGT ATTAATTCCA GTTCATTTAA TCCAGAGTGC 
TCCACACCTA AAAAGACTGC AAAAAAGAGA GTCTCCACTG ATGGTTCAGA AACAAAAAAT 
AAAGCTAAGC GTTATAATAA AACAACAAGA AAAGTAGTTC AATTAATACC ATTAGATGTA 
CATGGAAGAC CTATATTTCC TATTGCTTTG GGTGATCTCA CTATTTATTC TCTCGGTGAA 
GTAGTTTCCG ATAGAATTGC ATATCATACA GAAGATCTTA TTTATCCAGT AGGTTATTGC 
AGTACTAGAG TATATGCCAA CTTAAGAGAT GTAAGAACAA AAAGCTTATA TACATGTAAA 
ATATTGGATG GTGGACAGAA ACCAAGGTTT GAAATAGTAT CTGATAATGA CCTTGATCAA 
TCATTAGTGG GATCTACCCC TGATGAGTGT CATTCAAAGT TGTTAATGGC AATTTCTCCT 
GCACTTCGTT CAATAACTCC AAAGGGTGCT GATTTTTTTG GAATTTCACA CCCTACTGTA 
CAAAATCTCA TACAAAGTAC ACCTGGGACG CGTAAGCTTG CTATGTACAA ACAACAACGA 
TTTGAAGTAA GTAAAAATCA ATCAGTGGAA AGAGGAACAA GTCCAATAAC AGAAGAAGAA 
ACAGATCCAG GTCTTGGATT TGCTGCTTTA CATAGGCATT ATGCTTTAAC AAACTCTTAT 
CGAATCAAAG AAGAGCCAGC TGATAAAGAC CTTTTAACTC TTCAAGAATT TCTAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003705592.1
CDS	226..1245
Translation	MTQAYDNYYNDFQKNAELQQNLKYKKKYRRLKRIIKDTVFENAALCDQVAQMQENLMLVKEERLFLLRKLCQQQGDIDPSSMVARSQSNSINSSSFNPECSTPKKTAKKRVSTDGSETKNKAKRYNKTTRKVVQLIPLDVHGRPIFPIALGDLTIYSLGEVVSDRIAYHTEDLIYPVGYCSTRVYANLRDVRTKSLYTCKILDGGQKPRFEIVSDNDLDQSLVGSTPDECHSKLLMAISPALRSITPKGADFFGISHPTVQNLIQSTPGTRKLAMYKQQRFEVSKNQSVERGTSPITEEETDPGLGFAALHRHYALTNSYRIKEEPADKDLLTLQEFLA

Target ORF information:

RefSeq Version	XM_003705544.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata transforming growth factor beta regulator 1 (LOC100882084), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003705544.2

ORF Insert Sequence:

ATGACACAGG CTTATGATAA TTATTATAAT GATTTTCAAA AGAATGCCGA ATTACAACAA 
AATCTGAAAT ATAAAAAGAA ATATCGAAGA TTAAAGCGAA TCATTAAGGA TACAGTATTC 
GAAAATGCAG CTCTTTGTGA TCAAGTAGCA CAAATGCAAG AAAATCTTAT GTTAGTGAAA 
GAGGAAAGAT TATTCCTTTT ACGTAAATTA TGTCAACAAC AAGGAGATAT AGATCCTTCC 
TCTATGGTAG CTCGATCTCA GTCAAATAGT ATTAATTCCA GTTCATTTAA TCCAGAGTGC 
TCCACACCTA AAAAGACTGC AAAAAAGAGA GTCTCCACTG ATGGTTCAGA AACAAAAAAT 
AAAGCTAAGC GTTATAATAA AACAACAAGA AAAGTAGTTC AATTAATACC ATTAGATGTA 
CATGGAAGAC CTATATTTCC TATTGCTTTG GGTGATCTCA CTATTTATTC TCTCGGTGAA 
GTAGTTTCCG ATAGAATTGC ATATCATACA GAAGATCTTA TTTATCCAGT AGGTTATTGC 
AGTACTAGAG TATATGCCAA CTTAAGAGAT GTAAGAACAA AAAGCTTATA TACATGTAAA 
ATATTGGATG GTGGACAGAA ACCAAGGTTT GAAATAGTAT CTGATAATGA CCTTGATCAA 
TCATTAGTGG GATCTACCCC TGATGAGTGT CATTCAAAGT TGTTAATGGC AATTTCTCCT 
GCACTTCGTT CAATAACTCC AAAGGGTGCT GATTTTTTTG GAATTTCACA CCCTACTGTA 
CAAAATCTCA TACAAAGTAC ACCTGGGACG CGTAAGCTTG CTATGTACAA ACAACAACGA 
TTTGAAGTAA GTAAAAATCA ATCAGTGGAA AGAGGAACAA GTCCAATAAC AGAAGAAGAA 
ACAGATCCAG GTCTTGGATT TGCTGCTTTA CATAGGCATT ATGCTTTAAC AAACTCTTAT 
CGAATCAAAG AAGAGCCAGC TGATAAAGAC CTTTTAACTC TTCAAGAATT TCTAGCATAA