MTR_3g465600 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTR_3g465600
Entrez Gene ID	25490984
Full Name	Rab3 GTPase-activating protein catalytic subunit
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013604897.2	XP_013460351.1	rab3 GTPase-activating protein catalytic subunit
XM_013604897.1	XP_013460351.1	Rab3 GTPase-activating protein catalytic subunit

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MTR_3g465600 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTR_3g465600 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe196621	XM_013604897.2 Latest version!	Medicago truncatula rab3 GTPase-activating protein catalytic subunit (LOC25490984), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OMe196621	XM_013604897.1	Medicago truncatula Rab3 GTPase-activating protein catalytic subunit mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTR_3g465600 ( XM_013604897.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013460351.1 Medicago truncatula rab3 GTPase-activating protein catalytic subunit (LOC25490984), mRNA.

Price & Availability↑

CloneID	OMe196621
Clone ID Related Accession (Same CDS sequence)	XM_013604897.2 , XM_013604897.1
Accession Version	XM_013604897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	rab3 GTPase-activating protein catalytic subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016409.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_013604897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCAT CCTCCACCAA CACCAACACC GGCAAGACGA AGATCCTACC TGATTCTACT 
GATGACGATG AACACACCGA AGTCGAAGAT ATGGAAGAGG ATGAAGATGA AGACCTCGAG 
CATTTCGATG ATTTCACCCT TGCTTCTTCG TGGGAACGGT TTATTTCTGA AATAGAGGCT 
GTTTGCCGGC TTTGGATGTC TGATGGGCCA AAAAATTTAA TGGTAAAAGG TGCAGTTCTC 
TTGGAGTATT CTGGTAATTT GTACAAGGTG ACATCTGAGA CGAAGTATGC CTTGAAAACT 
TACTGCATGG AGTATTATTT TGAGACCAAC CCTTCTGATG ACGCAGGCAA GCCTGCAAAT 
TGGAATTTGG ATTTGCACGA TCTGCAGTTA TGTTTCGGTG TAAAGGAATT TTTGGTGATT 
GCTCCTCAGA GTGCAAGTGG TGTAGTTCTT GATGCACCAG AGGCTAGTAA ACTGTTGAGT 
GCTGTTGCGA TTGCTTTGTC AAATTGTTCA AGTTTGTGGC CAGCATTTGT TCCAGTTCAC 
GATCCTTCTC GGAAAGCCTA TATTGGAATT CAGAGCATGG GCACTGTATT CACTAGAAGG 
TTTGAAGCAG ATCGCATTGG TAGTCAGGTT CCAATAAAAC TTATGCATTT GGAGGGGCTG 
TACGAATTAT TTGTATCCAA ATTTGCATAC TCCACATTGG ATCTATCTGT TCATAATTTC 
AAAGTCCGAA TTGCAATGAA GCTAACATTC AGAACACTCC CCTTTGATGA GGACTATATG 
AAAGACTTTG ATTCTAAAAT AAACAAATCT GAAGAAAATC TTACTGGTGA GACATCGAGT 
GGGACACAAT GGGATGATGA TTGTTCTTGG AGTGAGTGGT ATTCTGCAGA AGATCCTGTT 
AAAGGTTTTG AACTGATCAC AATATGGTCA GAGAAAATGG TTGAAAGTTC TATGGAAATG 
GCTGAACTGG AAAATGCTTC ACCTCATGAA GCTGAGAAGT GGTTGATCTC TCTAAGACTT 
GAAGGTTCTA AAGAGAGTCG GGTTGGATTT GCCTCCCAGT TGCATCTTCT TGTTGATGCA 
TTGCAAATGT CATTTGAGGC ACACTTTATA GAAGATTTTG TTTCAGCTGA AAATCCTGGT 
TCCGATAATT TGAAATCCTC AATGGTTATA CCTTCACCAG CTGTTAGAGA TCGTGTGCTG 
AAAGAACTGT TTATTGAAGG CGTACAATTC TCAGATTTTA CTGATGGCGG ATATAAGACT 
TCGCGATCTG TAAAAGGCGC ACCTCTTGAG TCACTCTTTG CACAGTTTTG TTTACATTCT 
CTTTGGTTTG GCAATTGCAA TATACGTGCT ATTTCTGTGC TATGGATAGA ATTTGTTCGA 
GAGGTTAGGT GGTGTTGGGA AGAGTCACAA CCATTACCTA GGATGGCACC TAACGGATCA 
ATAGACCTTT CTACTTCTTT GATTTATCAG AAACTTCAGA TGCTTGCAAT ATGCATCGAG 
AGGAAGTGTC AGATGAATGA AGATTATCAG GACTGTATCG GAAGTATTGA TCATATAGAT 
TCCATGACTG AGGAAGAAAG TGTGGTTGGG GATGACTTGT TGAGTATACA GACACCCAGT 
GAGATTTTTC CCGGGAAAGT TGACAGAAAA CCTGAAGATA TGGACCTGTA TAAAGACAAA 
AAATCTTCAA ACTTTACTAG GAGAGGTTCA GCTGGTATTG TTGATTCTAT GATGCTTCTC 
AAGTCATATC AAAGTATGCA TGCCCCATAC ACACAGGAAC CACCTCTTAT GACAGAAGAT 
ATGCATGAAG AGAGGATGCA AGCTGTTGAA GCCATTGGTG ATTCATTTAA CTTTTCTGCT 
CAGCTGGAAA AGGATATCCT AACTTCAGAT ATGTCAGCAT TTAAAGCTGC GAATCCAGAT 
GCTATTTTTG AAGACTTTAT TAGGTGGCAT TCACCTGGAG ATTGGGAAGC GGATGATGAT 
CCCGAGAGCA GTGGATCAGC ATCATCCAAT GCTCTTGAAA TCAACAAGTC GAAAGATAGT 
TGGCCTCCAC GTGGGAAGCT TTCTAAGAGA ATGTCGGAGC ACGGAAATAT GTGGAGAAAG 
ATTTGGAGTA GCTCACCTGC TCTTCCTGCT TCGGAACAGA AGCCTCTTCT TGATCCAAAT 
AGAGAAGGAG AAAAAGTTCT TCATTATCTT GAAACCTTAC GACCACATCA ATTGCTTGAA 
CAAATGGTGT CTACTGCCTT CAGAGCAGCA GCCGACACGC TAAGTCAGAC TAGTTATGGA 
GAACTGAAAC AGATGGAGAC TAAGATTCAA CAACTTTATC CTACCATGGC ATCTGCTTTG 
AGGCCTTTAC AAGTAAATCG ATTATCTGCT GACAGTGAGA CCATTGAAGA CTTCAGACGA 
CTATGTGTTG TTTTTGAACG TGTTGAAAAA TTACTGACTG TCGCAGCTTC TCTTCATCGC 
AAGCTTATAC GAGCACCACG GCTCTCGAGA GAAATTTTTA ATGATTACTA CAACTTTTAT 
ATTCCAACAA TGGGAACAAG CTTGATTGAA GAGGTTGTTG AAAAGGAATT TGACAAGAAA 
CAAGAAGTAA GGAACCATGA AAGAGAAGTG TTGTCAAATA TGTTTGTTCC TCCCACAGCT 
AATCAGTCTT GGAGAAAGGT TTTGAGCATG GGGAATCTTC TCAATGGTCA CGAACCAATC 
CTCAGGGAGA TTATTTTTTC ACTCCACGAC AGAGTGAGTG GTAATCACTA TGCAGCTCGT 
AGCGGTAGTG TTTCTCAAGA AATTGAAACT TACAGAATGT ATATATGTGG AACATCTAAT 
GACCTACGTG TAGCACTTTC GGTTGTCTCG TGTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013460351.1
CDS	149..3007
Translation	MASSSTNTNTGKTKILPDSTDDDEHTEVEDMEEDEDEDLEHFDDFTLASSWERFISEIEAVCRLWMSDGPKNLMVKGAVLLEYSGNLYKVTSETKYALKTYCMEYYFETNPSDDAGKPANWNLDLHDLQLCFGVKEFLVIAPQSASGVVLDAPEASKLLSAVAIALSNCSSLWPAFVPVHDPSRKAYIGIQSMGTVFTRRFEADRIGSQVPIKLMHLEGLYELFVSKFAYSTLDLSVHNFKVRIAMKLTFRTLPFDEDYMKDFDSKINKSEENLTGETSSGTQWDDDCSWSEWYSAEDPVKGFELITIWSEKMVESSMEMAELENASPHEAEKWLISLRLEGSKESRVGFASQLHLLVDALQMSFEAHFIEDFVSAENPGSDNLKSSMVIPSPAVRDRVLKELFIEGVQFSDFTDGGYKTSRSVKGAPLESLFAQFCLHSLWFGNCNIRAISVLWIEFVREVRWCWEESQPLPRMAPNGSIDLSTSLIYQKLQMLAICIERKCQMNEDYQDCIGSIDHIDSMTEEESVVGDDLLSIQTPSEIFPGKVDRKPEDMDLYKDKKSSNFTRRGSAGIVDSMMLLKSYQSMHAPYTQEPPLMTEDMHEERMQAVEAIGDSFNFSAQLEKDILTSDMSAFKAANPDAIFEDFIRWHSPGDWEADDDPESSGSASSNALEINKSKDSWPPRGKLSKRMSEHGNMWRKIWSSSPALPASEQKPLLDPNREGEKVLHYLETLRPHQLLEQMVSTAFRAAADTLSQTSYGELKQMETKIQQLYPTMASALRPLQVNRLSADSETIEDFRRLCVVFERVEKLLTVAASLHRKLIRAPRLSREIFNDYYNFYIPTMGTSLIEEVVEKEFDKKQEVRNHEREVLSNMFVPPTANQSWRKVLSMGNLLNGHEPILREIIFSLHDRVSGNHYAARSGSVSQEIETYRMYICGTSNDLRVALSVVSCD

Target ORF information:

RefSeq Version	XM_013604897.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula rab3 GTPase-activating protein catalytic subunit (LOC25490984), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013604897.2

ORF Insert Sequence:

ATGGCGTCAT CCTCCACCAA CACCAACACC GGCAAGACGA AGATCCTACC TGATTCTACT 
GATGACGATG AACACACCGA AGTCGAAGAT ATGGAAGAGG ATGAAGATGA AGACCTCGAG 
CATTTCGATG ATTTCACCCT TGCTTCTTCG TGGGAACGGT TTATTTCTGA AATAGAGGCT 
GTTTGCCGGC TTTGGATGTC TGATGGGCCA AAAAATTTAA TGGTAAAAGG TGCAGTTCTC 
TTGGAGTATT CTGGTAATTT GTACAAGGTG ACATCTGAGA CGAAGTATGC CTTGAAAACT 
TACTGCATGG AGTATTATTT TGAGACCAAC CCTTCTGATG ACGCAGGCAA GCCTGCAAAT 
TGGAATTTGG ATTTGCACGA TCTGCAGTTA TGTTTCGGTG TAAAGGAATT TTTGGTGATT 
GCTCCTCAGA GTGCAAGTGG TGTAGTTCTT GATGCACCAG AGGCTAGTAA ACTGTTGAGT 
GCTGTTGCGA TTGCTTTGTC AAATTGTTCA AGTTTGTGGC CAGCATTTGT TCCAGTTCAC 
GATCCTTCTC GGAAAGCCTA TATTGGAATT CAGAGCATGG GCACTGTATT CACTAGAAGG 
TTTGAAGCAG ATCGCATTGG TAGTCAGGTT CCAATAAAAC TTATGCATTT GGAGGGGCTG 
TACGAATTAT TTGTATCCAA ATTTGCATAC TCCACATTGG ATCTATCTGT TCATAATTTC 
AAAGTCCGAA TTGCAATGAA GCTAACATTC AGAACACTCC CCTTTGATGA GGACTATATG 
AAAGACTTTG ATTCTAAAAT AAACAAATCT GAAGAAAATC TTACTGGTGA GACATCGAGT 
GGGACACAAT GGGATGATGA TTGTTCTTGG AGTGAGTGGT ATTCTGCAGA AGATCCTGTT 
AAAGGTTTTG AACTGATCAC AATATGGTCA GAGAAAATGG TTGAAAGTTC TATGGAAATG 
GCTGAACTGG AAAATGCTTC ACCTCATGAA GCTGAGAAGT GGTTGATCTC TCTAAGACTT 
GAAGGTTCTA AAGAGAGTCG GGTTGGATTT GCCTCCCAGT TGCATCTTCT TGTTGATGCA 
TTGCAAATGT CATTTGAGGC ACACTTTATA GAAGATTTTG TTTCAGCTGA AAATCCTGGT 
TCCGATAATT TGAAATCCTC AATGGTTATA CCTTCACCAG CTGTTAGAGA TCGTGTGCTG 
AAAGAACTGT TTATTGAAGG CGTACAATTC TCAGATTTTA CTGATGGCGG ATATAAGACT 
TCGCGATCTG TAAAAGGCGC ACCTCTTGAG TCACTCTTTG CACAGTTTTG TTTACATTCT 
CTTTGGTTTG GCAATTGCAA TATACGTGCT ATTTCTGTGC TATGGATAGA ATTTGTTCGA 
GAGGTTAGGT GGTGTTGGGA AGAGTCACAA CCATTACCTA GGATGGCACC TAACGGATCA 
ATAGACCTTT CTACTTCTTT GATTTATCAG AAACTTCAGA TGCTTGCAAT ATGCATCGAG 
AGGAAGTGTC AGATGAATGA AGATTATCAG GACTGTATCG GAAGTATTGA TCATATAGAT 
TCCATGACTG AGGAAGAAAG TGTGGTTGGG GATGACTTGT TGAGTATACA GACACCCAGT 
GAGATTTTTC CCGGGAAAGT TGACAGAAAA CCTGAAGATA TGGACCTGTA TAAAGACAAA 
AAATCTTCAA ACTTTACTAG GAGAGGTTCA GCTGGTATTG TTGATTCTAT GATGCTTCTC 
AAGTCATATC AAAGTATGCA TGCCCCATAC ACACAGGAAC CACCTCTTAT GACAGAAGAT 
ATGCATGAAG AGAGGATGCA AGCTGTTGAA GCCATTGGTG ATTCATTTAA CTTTTCTGCT 
CAGCTGGAAA AGGATATCCT AACTTCAGAT ATGTCAGCAT TTAAAGCTGC GAATCCAGAT 
GCTATTTTTG AAGACTTTAT TAGGTGGCAT TCACCTGGAG ATTGGGAAGC GGATGATGAT 
CCCGAGAGCA GTGGATCAGC ATCATCCAAT GCTCTTGAAA TCAACAAGTC GAAAGATAGT 
TGGCCTCCAC GTGGGAAGCT TTCTAAGAGA ATGTCGGAGC ACGGAAATAT GTGGAGAAAG 
ATTTGGAGTA GCTCACCTGC TCTTCCTGCT TCGGAACAGA AGCCTCTTCT TGATCCAAAT 
AGAGAAGGAG AAAAAGTTCT TCATTATCTT GAAACCTTAC GACCACATCA ATTGCTTGAA 
CAAATGGTGT CTACTGCCTT CAGAGCAGCA GCCGACACGC TAAGTCAGAC TAGTTATGGA 
GAACTGAAAC AGATGGAGAC TAAGATTCAA CAACTTTATC CTACCATGGC ATCTGCTTTG 
AGGCCTTTAC AAGTAAATCG ATTATCTGCT GACAGTGAGA CCATTGAAGA CTTCAGACGA 
CTATGTGTTG TTTTTGAACG TGTTGAAAAA TTACTGACTG TCGCAGCTTC TCTTCATCGC 
AAGCTTATAC GAGCACCACG GCTCTCGAGA GAAATTTTTA ATGATTACTA CAACTTTTAT 
ATTCCAACAA TGGGAACAAG CTTGATTGAA GAGGTTGTTG AAAAGGAATT TGACAAGAAA 
CAAGAAGTAA GGAACCATGA AAGAGAAGTG TTGTCAAATA TGTTTGTTCC TCCCACAGCT 
AATCAGTCTT GGAGAAAGGT TTTGAGCATG GGGAATCTTC TCAATGGTCA CGAACCAATC 
CTCAGGGAGA TTATTTTTTC ACTCCACGAC AGAGTGAGTG GTAATCACTA TGCAGCTCGT 
AGCGGTAGTG TTTCTCAAGA AATTGAAACT TACAGAATGT ATATATGTGG AACATCTAAT 
GACCTACGTG TAGCACTTTC GGTTGTCTCG TGTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR_3g465600 ( XM_013604897.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013460351.1 Medicago truncatula Rab3 GTPase-activating protein catalytic subunit mRNA.

Price & Availability↑

CloneID	OMe196621
Clone ID Related Accession (Same CDS sequence)	XM_013604897.2 , XM_013604897.1
Accession Version	XM_013604897.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	Rab3 GTPase-activating protein catalytic subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016409). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGGCGTCAT CCTCCACCAA CACCAACACC GGCAAGACGA AGATCCTACC TGATTCTACT 
GATGACGATG AACACACCGA AGTCGAAGAT ATGGAAGAGG ATGAAGATGA AGACCTCGAG 
CATTTCGATG ATTTCACCCT TGCTTCTTCG TGGGAACGGT TTATTTCTGA AATAGAGGCT 
GTTTGCCGGC TTTGGATGTC TGATGGGCCA AAAAATTTAA TGGTAAAAGG TGCAGTTCTC 
TTGGAGTATT CTGGTAATTT GTACAAGGTG ACATCTGAGA CGAAGTATGC CTTGAAAACT 
TACTGCATGG AGTATTATTT TGAGACCAAC CCTTCTGATG ACGCAGGCAA GCCTGCAAAT 
TGGAATTTGG ATTTGCACGA TCTGCAGTTA TGTTTCGGTG TAAAGGAATT TTTGGTGATT 
GCTCCTCAGA GTGCAAGTGG TGTAGTTCTT GATGCACCAG AGGCTAGTAA ACTGTTGAGT 
GCTGTTGCGA TTGCTTTGTC AAATTGTTCA AGTTTGTGGC CAGCATTTGT TCCAGTTCAC 
GATCCTTCTC GGAAAGCCTA TATTGGAATT CAGAGCATGG GCACTGTATT CACTAGAAGG 
TTTGAAGCAG ATCGCATTGG TAGTCAGGTT CCAATAAAAC TTATGCATTT GGAGGGGCTG 
TACGAATTAT TTGTATCCAA ATTTGCATAC TCCACATTGG ATCTATCTGT TCATAATTTC 
AAAGTCCGAA TTGCAATGAA GCTAACATTC AGAACACTCC CCTTTGATGA GGACTATATG 
AAAGACTTTG ATTCTAAAAT AAACAAATCT GAAGAAAATC TTACTGGTGA GACATCGAGT 
GGGACACAAT GGGATGATGA TTGTTCTTGG AGTGAGTGGT ATTCTGCAGA AGATCCTGTT 
AAAGGTTTTG AACTGATCAC AATATGGTCA GAGAAAATGG TTGAAAGTTC TATGGAAATG 
GCTGAACTGG AAAATGCTTC ACCTCATGAA GCTGAGAAGT GGTTGATCTC TCTAAGACTT 
GAAGGTTCTA AAGAGAGTCG GGTTGGATTT GCCTCCCAGT TGCATCTTCT TGTTGATGCA 
TTGCAAATGT CATTTGAGGC ACACTTTATA GAAGATTTTG TTTCAGCTGA AAATCCTGGT 
TCCGATAATT TGAAATCCTC AATGGTTATA CCTTCACCAG CTGTTAGAGA TCGTGTGCTG 
AAAGAACTGT TTATTGAAGG CGTACAATTC TCAGATTTTA CTGATGGCGG ATATAAGACT 
TCGCGATCTG TAAAAGGCGC ACCTCTTGAG TCACTCTTTG CACAGTTTTG TTTACATTCT 
CTTTGGTTTG GCAATTGCAA TATACGTGCT ATTTCTGTGC TATGGATAGA ATTTGTTCGA 
GAGGTTAGGT GGTGTTGGGA AGAGTCACAA CCATTACCTA GGATGGCACC TAACGGATCA 
ATAGACCTTT CTACTTCTTT GATTTATCAG AAACTTCAGA TGCTTGCAAT ATGCATCGAG 
AGGAAGTGTC AGATGAATGA AGATTATCAG GACTGTATCG GAAGTATTGA TCATATAGAT 
TCCATGACTG AGGAAGAAAG TGTGGTTGGG GATGACTTGT TGAGTATACA GACACCCAGT 
GAGATTTTTC CCGGGAAAGT TGACAGAAAA CCTGAAGATA TGGACCTGTA TAAAGACAAA 
AAATCTTCAA ACTTTACTAG GAGAGGTTCA GCTGGTATTG TTGATTCTAT GATGCTTCTC 
AAGTCATATC AAAGTATGCA TGCCCCATAC ACACAGGAAC CACCTCTTAT GACAGAAGAT 
ATGCATGAAG AGAGGATGCA AGCTGTTGAA GCCATTGGTG ATTCATTTAA CTTTTCTGCT 
CAGCTGGAAA AGGATATCCT AACTTCAGAT ATGTCAGCAT TTAAAGCTGC GAATCCAGAT 
GCTATTTTTG AAGACTTTAT TAGGTGGCAT TCACCTGGAG ATTGGGAAGC GGATGATGAT 
CCCGAGAGCA GTGGATCAGC ATCATCCAAT GCTCTTGAAA TCAACAAGTC GAAAGATAGT 
TGGCCTCCAC GTGGGAAGCT TTCTAAGAGA ATGTCGGAGC ACGGAAATAT GTGGAGAAAG 
ATTTGGAGTA GCTCACCTGC TCTTCCTGCT TCGGAACAGA AGCCTCTTCT TGATCCAAAT 
AGAGAAGGAG AAAAAGTTCT TCATTATCTT GAAACCTTAC GACCACATCA ATTGCTTGAA 
CAAATGGTGT CTACTGCCTT CAGAGCAGCA GCCGACACGC TAAGTCAGAC TAGTTATGGA 
GAACTGAAAC AGATGGAGAC TAAGATTCAA CAACTTTATC CTACCATGGC ATCTGCTTTG 
AGGCCTTTAC AAGTAAATCG ATTATCTGCT GACAGTGAGA CCATTGAAGA CTTCAGACGA 
CTATGTGTTG TTTTTGAACG TGTTGAAAAA TTACTGACTG TCGCAGCTTC TCTTCATCGC 
AAGCTTATAC GAGCACCACG GCTCTCGAGA GAAATTTTTA ATGATTACTA CAACTTTTAT 
ATTCCAACAA TGGGAACAAG CTTGATTGAA GAGGTTGTTG AAAAGGAATT TGACAAGAAA 
CAAGAAGTAA GGAACCATGA AAGAGAAGTG TTGTCAAATA TGTTTGTTCC TCCCACAGCT 
AATCAGTCTT GGAGAAAGGT TTTGAGCATG GGGAATCTTC TCAATGGTCA CGAACCAATC 
CTCAGGGAGA TTATTTTTTC ACTCCACGAC AGAGTGAGTG GTAATCACTA TGCAGCTCGT 
AGCGGTAGTG TTTCTCAAGA AATTGAAACT TACAGAATGT ATATATGTGG AACATCTAAT 
GACCTACGTG TAGCACTTTC GGTTGTCTCG TGTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013460351.1
CDS	152..3010
Translation	MASSSTNTNTGKTKILPDSTDDDEHTEVEDMEEDEDEDLEHFDDFTLASSWERFISEIEAVCRLWMSDGPKNLMVKGAVLLEYSGNLYKVTSETKYALKTYCMEYYFETNPSDDAGKPANWNLDLHDLQLCFGVKEFLVIAPQSASGVVLDAPEASKLLSAVAIALSNCSSLWPAFVPVHDPSRKAYIGIQSMGTVFTRRFEADRIGSQVPIKLMHLEGLYELFVSKFAYSTLDLSVHNFKVRIAMKLTFRTLPFDEDYMKDFDSKINKSEENLTGETSSGTQWDDDCSWSEWYSAEDPVKGFELITIWSEKMVESSMEMAELENASPHEAEKWLISLRLEGSKESRVGFASQLHLLVDALQMSFEAHFIEDFVSAENPGSDNLKSSMVIPSPAVRDRVLKELFIEGVQFSDFTDGGYKTSRSVKGAPLESLFAQFCLHSLWFGNCNIRAISVLWIEFVREVRWCWEESQPLPRMAPNGSIDLSTSLIYQKLQMLAICIERKCQMNEDYQDCIGSIDHIDSMTEEESVVGDDLLSIQTPSEIFPGKVDRKPEDMDLYKDKKSSNFTRRGSAGIVDSMMLLKSYQSMHAPYTQEPPLMTEDMHEERMQAVEAIGDSFNFSAQLEKDILTSDMSAFKAANPDAIFEDFIRWHSPGDWEADDDPESSGSASSNALEINKSKDSWPPRGKLSKRMSEHGNMWRKIWSSSPALPASEQKPLLDPNREGEKVLHYLETLRPHQLLEQMVSTAFRAAADTLSQTSYGELKQMETKIQQLYPTMASALRPLQVNRLSADSETIEDFRRLCVVFERVEKLLTVAASLHRKLIRAPRLSREIFNDYYNFYIPTMGTSLIEEVVEKEFDKKQEVRNHEREVLSNMFVPPTANQSWRKVLSMGNLLNGHEPILREIIFSLHDRVSGNHYAARSGSVSQEIETYRMYICGTSNDLRVALSVVSCD

Target ORF information:

RefSeq Version	XM_013604897.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula Rab3 GTPase-activating protein catalytic subunit mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013604897.1

ORF Insert Sequence:

ATGGCGTCAT CCTCCACCAA CACCAACACC GGCAAGACGA AGATCCTACC TGATTCTACT 
GATGACGATG AACACACCGA AGTCGAAGAT ATGGAAGAGG ATGAAGATGA AGACCTCGAG 
CATTTCGATG ATTTCACCCT TGCTTCTTCG TGGGAACGGT TTATTTCTGA AATAGAGGCT 
GTTTGCCGGC TTTGGATGTC TGATGGGCCA AAAAATTTAA TGGTAAAAGG TGCAGTTCTC 
TTGGAGTATT CTGGTAATTT GTACAAGGTG ACATCTGAGA CGAAGTATGC CTTGAAAACT 
TACTGCATGG AGTATTATTT TGAGACCAAC CCTTCTGATG ACGCAGGCAA GCCTGCAAAT 
TGGAATTTGG ATTTGCACGA TCTGCAGTTA TGTTTCGGTG TAAAGGAATT TTTGGTGATT 
GCTCCTCAGA GTGCAAGTGG TGTAGTTCTT GATGCACCAG AGGCTAGTAA ACTGTTGAGT 
GCTGTTGCGA TTGCTTTGTC AAATTGTTCA AGTTTGTGGC CAGCATTTGT TCCAGTTCAC 
GATCCTTCTC GGAAAGCCTA TATTGGAATT CAGAGCATGG GCACTGTATT CACTAGAAGG 
TTTGAAGCAG ATCGCATTGG TAGTCAGGTT CCAATAAAAC TTATGCATTT GGAGGGGCTG 
TACGAATTAT TTGTATCCAA ATTTGCATAC TCCACATTGG ATCTATCTGT TCATAATTTC 
AAAGTCCGAA TTGCAATGAA GCTAACATTC AGAACACTCC CCTTTGATGA GGACTATATG 
AAAGACTTTG ATTCTAAAAT AAACAAATCT GAAGAAAATC TTACTGGTGA GACATCGAGT 
GGGACACAAT GGGATGATGA TTGTTCTTGG AGTGAGTGGT ATTCTGCAGA AGATCCTGTT 
AAAGGTTTTG AACTGATCAC AATATGGTCA GAGAAAATGG TTGAAAGTTC TATGGAAATG 
GCTGAACTGG AAAATGCTTC ACCTCATGAA GCTGAGAAGT GGTTGATCTC TCTAAGACTT 
GAAGGTTCTA AAGAGAGTCG GGTTGGATTT GCCTCCCAGT TGCATCTTCT TGTTGATGCA 
TTGCAAATGT CATTTGAGGC ACACTTTATA GAAGATTTTG TTTCAGCTGA AAATCCTGGT 
TCCGATAATT TGAAATCCTC AATGGTTATA CCTTCACCAG CTGTTAGAGA TCGTGTGCTG 
AAAGAACTGT TTATTGAAGG CGTACAATTC TCAGATTTTA CTGATGGCGG ATATAAGACT 
TCGCGATCTG TAAAAGGCGC ACCTCTTGAG TCACTCTTTG CACAGTTTTG TTTACATTCT 
CTTTGGTTTG GCAATTGCAA TATACGTGCT ATTTCTGTGC TATGGATAGA ATTTGTTCGA 
GAGGTTAGGT GGTGTTGGGA AGAGTCACAA CCATTACCTA GGATGGCACC TAACGGATCA 
ATAGACCTTT CTACTTCTTT GATTTATCAG AAACTTCAGA TGCTTGCAAT ATGCATCGAG 
AGGAAGTGTC AGATGAATGA AGATTATCAG GACTGTATCG GAAGTATTGA TCATATAGAT 
TCCATGACTG AGGAAGAAAG TGTGGTTGGG GATGACTTGT TGAGTATACA GACACCCAGT 
GAGATTTTTC CCGGGAAAGT TGACAGAAAA CCTGAAGATA TGGACCTGTA TAAAGACAAA 
AAATCTTCAA ACTTTACTAG GAGAGGTTCA GCTGGTATTG TTGATTCTAT GATGCTTCTC 
AAGTCATATC AAAGTATGCA TGCCCCATAC ACACAGGAAC CACCTCTTAT GACAGAAGAT 
ATGCATGAAG AGAGGATGCA AGCTGTTGAA GCCATTGGTG ATTCATTTAA CTTTTCTGCT 
CAGCTGGAAA AGGATATCCT AACTTCAGAT ATGTCAGCAT TTAAAGCTGC GAATCCAGAT 
GCTATTTTTG AAGACTTTAT TAGGTGGCAT TCACCTGGAG ATTGGGAAGC GGATGATGAT 
CCCGAGAGCA GTGGATCAGC ATCATCCAAT GCTCTTGAAA TCAACAAGTC GAAAGATAGT 
TGGCCTCCAC GTGGGAAGCT TTCTAAGAGA ATGTCGGAGC ACGGAAATAT GTGGAGAAAG 
ATTTGGAGTA GCTCACCTGC TCTTCCTGCT TCGGAACAGA AGCCTCTTCT TGATCCAAAT 
AGAGAAGGAG AAAAAGTTCT TCATTATCTT GAAACCTTAC GACCACATCA ATTGCTTGAA 
CAAATGGTGT CTACTGCCTT CAGAGCAGCA GCCGACACGC TAAGTCAGAC TAGTTATGGA 
GAACTGAAAC AGATGGAGAC TAAGATTCAA CAACTTTATC CTACCATGGC ATCTGCTTTG 
AGGCCTTTAC AAGTAAATCG ATTATCTGCT GACAGTGAGA CCATTGAAGA CTTCAGACGA 
CTATGTGTTG TTTTTGAACG TGTTGAAAAA TTACTGACTG TCGCAGCTTC TCTTCATCGC 
AAGCTTATAC GAGCACCACG GCTCTCGAGA GAAATTTTTA ATGATTACTA CAACTTTTAT 
ATTCCAACAA TGGGAACAAG CTTGATTGAA GAGGTTGTTG AAAAGGAATT TGACAAGAAA 
CAAGAAGTAA GGAACCATGA AAGAGAAGTG TTGTCAAATA TGTTTGTTCC TCCCACAGCT 
AATCAGTCTT GGAGAAAGGT TTTGAGCATG GGGAATCTTC TCAATGGTCA CGAACCAATC 
CTCAGGGAGA TTATTTTTTC ACTCCACGAC AGAGTGAGTG GTAATCACTA TGCAGCTCGT 
AGCGGTAGTG TTTCTCAAGA AATTGAAACT TACAGAATGT ATATATGTGG AACATCTAAT 
GACCTACGTG TAGCACTTTC GGTTGTCTCG TGTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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MTR_3g465600 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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