LOC11443135 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC11443135
Entrez Gene ID	11443135
Full Name	abnormal spindle-like microcephaly-associated-like protein, putative
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003610366.2	XP_003610414.2	abnormal spindle-like microcephaly-associated-like protein, putative
XM_024782542.1	XP_024638310.1	abnormal spindle-like microcephaly-associated protein homolog isoform X1
XM_003610366.3	XP_003610414.2	abnormal spindle-like microcephaly-associated protein homolog isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC11443135 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC11443135 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe185979	XM_003610366.3 Latest version!	Medicago truncatula abnormal spindle-like microcephaly-associated protein homolog (LOC11443135), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OMe264161	XM_024782542.1 Latest version!	Medicago truncatula abnormal spindle-like microcephaly-associated protein homolog (LOC11443135), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OMe185979	XM_003610366.2	Medicago truncatula abnormal spindle-like microcephaly-associated-like protein, putative mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC11443135 ( XM_003610366.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003610414.2 Medicago truncatula abnormal spindle-like microcephaly-associated-like protein, putative mRNA.

Price & Availability↑

CloneID	OMe185979
Clone ID Related Accession (Same CDS sequence)	XM_003610366.2 , XM_003610366.3
Accession Version	XM_003610366.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	abnormal spindle-like microcephaly-associated-like protein, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016410). On Aug 25, 2015 this sequence version replaced XM_003610366.1. ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGGAAGAAG GTAACAACAA CAACGAGCTT CCATGTCCAT CACCTTCACC TTTTCGCTCT 
TCTTCACTTT TCAAAGACAT TTCTAACTTC AAAACCCCCA AACGCGCTTC TTCTTCTTCT 
TCTTTTCTCT CTCAACAACA TACTCCTCAA ACTCAATTTT TCACCGCTTC CAAATCCAAA 
CACACCACAA CACTCCCGAG GAGGCCGAGA CTCAAAGAAA ATCATACCAT TTCAACTGCT 
TCAAAGAAAC TCAAAGCTTT TCAACTCGAA CAATCTCACT CCTCCAGAAT CGAACAAATC 
AAACAACAAC AATCGCTTAA ATCGTTAGCC AAATCACTCA CTGTTTGGCT TAATTTCCTT 
CTCGAATCAC CTGCTTCTTG CGGTTGCGAT GTTTCAATCG CCGGTGGAAG TCAGATCGCA 
GATGCTTCTC CGGTGACGAG TAAGGGGAAA CGTGATAATG TTCCGGGAAA TTCATTTGGA 
GTTGATTCTA CTTGGAGAAC TCCGAAACGA CAGAGGAAGA CGACGATGAC AACTTCTTCG 
TCGCGTTTTG GTAAGGAGAA TGTGTCTGCT GTGGATATGC AGAATTCATC GTTTTCGCGT 
TTGAAGGATT CTTTGAAGGA TGTATGTAGC TTCGATGATT TTAAGCAGCG GATGAGTGTT 
TATTTGAGTC TCGGAACTTG TGAAGATATT TTCCATGTCA TGAATCAAGT CACTAAGACA 
ATTGATGAAG GAAGGCTAAA TATGAAGGCA CATTGCCCGA TCGTGACGGA TTTAGGGTTG 
AAGGATAAGG CCATAAAAGT CCTTATGTGT TATAATCCAA GTTGGCTGCG AATTGGTTTA 
TATATAATTT TCGGTGGGGA CTCTTTAGTG TCAAATGGGG ATGGTGATTC TGACAAAGAT 
GTTACATTTT TAAAAATGGT CATTGATAAA CTGTTTTTTA CACACGAAGG TTTCGCAAAA 
TCATATGCAT ATAACAAGAT GGTTGAAGGA GTTTATAGGT CTGGTTACTA TGAAAATTTG 
GGGAATGTGA TTTTGAAGAG AATACTGTTG CTTGTGCTTA TACTTGATAA AGCTAAGTGT 
CAAAGTTGCC TCCCGATTGA GTATGGTATT GACGGACTGG ACAGTGGTTC CCCTTTATTA 
TTCAAAGCAG AATCTTGGGT TAAAGCAAGT AGTCAAGTGA TCCAGGAATT TCTGTCATCT 
GATGTAATGC GCGGAGAAGG CAATCTTCTA ACACATCTGG TTATTTTAGG TTACAAACTA 
ACTCACCAAC AGGGACCTCT TGTTGAGTAT GATTTCCGAG TCAAGGATTT ATTTATAGAT 
CTCCAAGATG GGCTAAAATT GTGTAGAGCT ATTCATCTCT TGCAAAATGA TTCTTCTATA 
CTCAAGAAAA TTGCAGTTCC TTCTGACACC CGTAAGAAAA ATATGGTAAA CAGTGGTGTT 
GCCCTACAAT ATCTTAGACT AGCCGGGGTC TCACTACTTG ATGAAGACGG CACAATGATT 
GTAGCAGATG ATATTGTTAA TGGAGACAGA GAACTCACAA TTTCTTTACT CTGGAATATG 
TTCATTCACT TGCAGCTACC TTTGTTGGTT GATAAAACAA GTTTAGTTGG GGAAATTTCA 
AAAATTCGCG GACTTGGCAC GGAACTCATG ACCGGTGCAA ATTCTAGTTC TCTGGAATTG 
CTTTTGAAAT GGATTCAGGC TGTTTGTGAC CATTATAACT GCCCAGTTGA CAATTTTCAC 
TCCCTTGTTG ATGGCAAAGC AATCTGGTGC TTACTTGATC ATTACTTCCA GAAGGAACTT 
CACAATGTTT GTTCGTTGAA GGAATTTTAT GAAAAAAGTG GCAAGACATC AATTATGTCA 
GTGAATGAGT ATTCAGATGC ACTGTACAAT TTCATATTGT CCCAGAAATT GACCACATTA 
CTGGGGAATT TTCCTGAGGT ACTTCAGATT AGTGAGCTAC TTCAATATAA TGGTGCTTGC 
AGCGATCGAA GTGTGGTGAT ATTGGTTGTT TTCCTGGCAA GCCAGTTATT TGTCAAGGAA 
AAAGTGGACA ACTTAAATTT TCATAAGCTA TTAGGTTATG ACTGCCAAAA CACAAATCGC 
AGGAACTTGA GGACTGTACA GTGCCATTCC CGTTCTGAAT CAGCACAAAA ACCTTATGAT 
TCAGATGTGG GTGACAATGA AGATACGGCA AGAAAATTCA AGGCCATTCA GACTTGGTGG 
CAAGATATGG CTGATCGAAA CTGTATTATG CAGCCAACTA TCTCTATTTT GCAGACATCT 
ATGACCACAG AATGCAACAC TTCTGTTAGA AGAGAAAATG CTGCAAGAAC AATACAATCA 
CGTATCCGAG GACTGGTTGT ACACCGCAAG TTTCACAAGA TGGTAAATAG TGTTACCCTT 
TTGCAGACCG TTTTCAGAGC TTGGCTGAAA GTGAGGCAGG AGTCGGTCTG CATTATATTC 
ACTACCGGTC CAATCTATGA TTTCTCATGT GAAATATTGA AGAAATCCGA AGTATACGAG 
AAGTATGTCA TGCTTTTCTA CCAAAGGCAT TCTTTTTTGA GGTTGAAAAG ATCAGCGCAG 
CTTATCCAGC AAGCAGTGAG GAGTTGGTTC TATTGGAGGG CTCGACAAGG ATGTAGAAGT 
CCTGACCTCT TGACTGCAGA CACTGTGAAT GCTGCCACTT CTATTCAGAA ATTCCTTCGT 
GGTTGGATGG CACGATCTAG ATATATTTAT CTGCTAGATC AAAAAGAGAA AACTCTACAT 
TTAGCCGAAC AGAAACTTAT CTTTGATCTT AAAACAAAGG CAGCCATTGG CATTCAGGTT 
GCTTGGAAAA ACTACATACG TTGCAAGTCT ACTAGGAAGG AGCACTTGTT TGCAACTAAA 
ATTCAATGTA ATTTCCGTAG GTGGTTATTG AGAAAACGAT TTATAAATCA GATTCAAGCT 
GTCATAAAGA TTCAATCATA TTTTCGAATG TGGAGATGTG TGATTGCTAT TCAGAACTTC 
AAAACCATGT CTAAGGCTGC TATTGTCATT CAATCTTTTT TCCGTGGATG GATTGCTCGG 
AAGAATGCTT GTGCTCGTAA GAATCAAATT GTTGAGATTC AAAGGCATTG CCGTGGTTGG 
CTTGTGAAAC GGAATTTCTT GTTTCAAAGA GATGCTATAG TAAAGATCCA GAGTGTCAGT 
CGAAGTTTGA AATGCCAGAA GACACTCAAT TGTCAGAAAG ATGCTGCACT GGAGATCCAG 
CGCTTCATCA GGGGACATTT AACCCGAAAT CGGCTATTAG GTAGTGCTTT AAGGTTACTT 
TCAGCTGATA CTGGTAGTTG CATTTCAAGA CCAGCTGGCT TATGTAGTTT TCAACTTGAA 
GCATTCATGT TCGCAGTAGT GAAATTGCAA CGGTGGTGGA GGGGTCTCTT GTTGCTTAAA 
TTAATGACTA GATCGGCCAT CATAATTCAA TCTTGTACTC GTGGGTGGAT AGCCAGACGG 
AAGGCCATAG TTGAGACACA GAATATTAAT GTCATGGAAG AGTATGCCGC ATTGGAGCTC 
CAGCGCTATA TTAGGGGGCA TTTAACTCGA AATTTGATAT TAGGCAGTGC TTCTAAGCTA 
CGTGCAGTTG CTGCTGGATG CATTTCAAAA CGAACTGGCT TTTGTAGTTT TCAGTTGGAA 
CTATTCTTGT TTCAAGTAGT GAAATTGCAA CGATGGTGGA AGCATCTCTT GTTACATAAA 
TTGATGACAA AGTCTGCAAT CATTATACAG TCCCACATTC GTGGGTGGGC AGCTAGGCGA 
AAGGCGGTTG TTTATAGGCA TCATATCGTT GTCATACAGT CCCACTGGAA AGGTTACGTT 
GCACGTCAGC AGTCAACAAA ACAATTAATG GATTTGCGCT CAAGACTTCA AGAGTCTTCC 
AAAAATGTAG ATGACAGCAA GCGTCTCATT AACAGACTCC TGGCAGCGCT TTCAGAGCTG 
CTTAGTATGA AGAGTCTTAG TGACATTCTT CATACTTGTT CAACGTTGGA TTTGGCAACA 
TGGCATTCTC AGAGATGTTG TGAAGAACTT GTGGCTGCTG GGGCCATTGA CACCTTGCTG 
CGGCTTATTC GATTAATCAG CAGGAGTATC CCTGATCAAG AGGTTTTAAA GCATGTTTTG 
TCAACTCTCC GAAATCTTGC TCGCTATCCT CATCTGCTAG AAGTGCTGAT ACAAAGACAG 
GGTTCTATAC AGACAATCGT CTTGGAGTTA CTGAGGAATA AGGAGGAGGG CTTCTTCATT 
GCTTCTGAGC TTTTGAAGAA GATATGCTCA ACTCAAAAAG GCGTTGAGAC CATACTCAAA 
TCTCCTGCCT TTCTAAAGAG ACTGCACAGT CTTGTTGAGG AGCTTACTAG GAAGGCCACT 
TACCAGAAAA GAAATGTTCG GGGTCCTACT CCTAGTTCAA TTGTTATTGT TAGAGAAAAC 
ACAGATAGAA GATTGAAGGA GGTTACTGAG ATTCTGAAGC TACTAGCACA CCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003610414.2
CDS	102..4598
Translation	MEEGNNNNELPCPSPSPFRSSSLFKDISNFKTPKRASSSSSFLSQQHTPQTQFFTASKSKHTTTLPRRPRLKENHTISTASKKLKAFQLEQSHSSRIEQIKQQQSLKSLAKSLTVWLNFLLESPASCGCDVSIAGGSQIADASPVTSKGKRDNVPGNSFGVDSTWRTPKRQRKTTMTTSSSRFGKENVSAVDMQNSSFSRLKDSLKDVCSFDDFKQRMSVYLSLGTCEDIFHVMNQVTKTIDEGRLNMKAHCPIVTDLGLKDKAIKVLMCYNPSWLRIGLYIIFGGDSLVSNGDGDSDKDVTFLKMVIDKLFFTHEGFAKSYAYNKMVEGVYRSGYYENLGNVILKRILLLVLILDKAKCQSCLPIEYGIDGLDSGSPLLFKAESWVKASSQVIQEFLSSDVMRGEGNLLTHLVILGYKLTHQQGPLVEYDFRVKDLFIDLQDGLKLCRAIHLLQNDSSILKKIAVPSDTRKKNMVNSGVALQYLRLAGVSLLDEDGTMIVADDIVNGDRELTISLLWNMFIHLQLPLLVDKTSLVGEISKIRGLGTELMTGANSSSLELLLKWIQAVCDHYNCPVDNFHSLVDGKAIWCLLDHYFQKELHNVCSLKEFYEKSGKTSIMSVNEYSDALYNFILSQKLTTLLGNFPEVLQISELLQYNGACSDRSVVILVVFLASQLFVKEKVDNLNFHKLLGYDCQNTNRRNLRTVQCHSRSESAQKPYDSDVGDNEDTARKFKAIQTWWQDMADRNCIMQPTISILQTSMTTECNTSVRRENAARTIQSRIRGLVVHRKFHKMVNSVTLLQTVFRAWLKVRQESVCIIFTTGPIYDFSCEILKKSEVYEKYVMLFYQRHSFLRLKRSAQLIQQAVRSWFYWRARQGCRSPDLLTADTVNAATSIQKFLRGWMARSRYIYLLDQKEKTLHLAEQKLIFDLKTKAAIGIQVAWKNYIRCKSTRKEHLFATKIQCNFRRWLLRKRFINQIQAVIKIQSYFRMWRCVIAIQNFKTMSKAAIVIQSFFRGWIARKNACARKNQIVEIQRHCRGWLVKRNFLFQRDAIVKIQSVSRSLKCQKTLNCQKDAALEIQRFIRGHLTRNRLLGSALRLLSADTGSCISRPAGLCSFQLEAFMFAVVKLQRWWRGLLLLKLMTRSAIIIQSCTRGWIARRKAIVETQNINVMEEYAALELQRYIRGHLTRNLILGSASKLRAVAAGCISKRTGFCSFQLELFLFQVVKLQRWWKHLLLHKLMTKSAIIIQSHIRGWAARRKAVVYRHHIVVIQSHWKGYVARQQSTKQLMDLRSRLQESSKNVDDSKRLINRLLAALSELLSMKSLSDILHTCSTLDLATWHSQRCCEELVAAGAIDTLLRLIRLISRSIPDQEVLKHVLSTLRNLARYPHLLEVLIQRQGSIQTIVLELLRNKEEGFFIASELLKKICSTQKGVETILKSPAFLKRLHSLVEELTRKATYQKRNVRGPTPSSIVIVRENTDRRLKEVTEILKLLAHP

Target ORF information:

RefSeq Version	XM_003610366.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula abnormal spindle-like microcephaly-associated-like protein, putative mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003610366.2

ORF Insert Sequence:

ATGGAAGAAG GTAACAACAA CAACGAGCTT CCATGTCCAT CACCTTCACC TTTTCGCTCT 
TCTTCACTTT TCAAAGACAT TTCTAACTTC AAAACCCCCA AACGCGCTTC TTCTTCTTCT 
TCTTTTCTCT CTCAACAACA TACTCCTCAA ACTCAATTTT TCACCGCTTC CAAATCCAAA 
CACACCACAA CACTCCCGAG GAGGCCGAGA CTCAAAGAAA ATCATACCAT TTCAACTGCT 
TCAAAGAAAC TCAAAGCTTT TCAACTCGAA CAATCTCACT CCTCCAGAAT CGAACAAATC 
AAACAACAAC AATCGCTTAA ATCGTTAGCC AAATCACTCA CTGTTTGGCT TAATTTCCTT 
CTCGAATCAC CTGCTTCTTG CGGTTGCGAT GTTTCAATCG CCGGTGGAAG TCAGATCGCA 
GATGCTTCTC CGGTGACGAG TAAGGGGAAA CGTGATAATG TTCCGGGAAA TTCATTTGGA 
GTTGATTCTA CTTGGAGAAC TCCGAAACGA CAGAGGAAGA CGACGATGAC AACTTCTTCG 
TCGCGTTTTG GTAAGGAGAA TGTGTCTGCT GTGGATATGC AGAATTCATC GTTTTCGCGT 
TTGAAGGATT CTTTGAAGGA TGTATGTAGC TTCGATGATT TTAAGCAGCG GATGAGTGTT 
TATTTGAGTC TCGGAACTTG TGAAGATATT TTCCATGTCA TGAATCAAGT CACTAAGACA 
ATTGATGAAG GAAGGCTAAA TATGAAGGCA CATTGCCCGA TCGTGACGGA TTTAGGGTTG 
AAGGATAAGG CCATAAAAGT CCTTATGTGT TATAATCCAA GTTGGCTGCG AATTGGTTTA 
TATATAATTT TCGGTGGGGA CTCTTTAGTG TCAAATGGGG ATGGTGATTC TGACAAAGAT 
GTTACATTTT TAAAAATGGT CATTGATAAA CTGTTTTTTA CACACGAAGG TTTCGCAAAA 
TCATATGCAT ATAACAAGAT GGTTGAAGGA GTTTATAGGT CTGGTTACTA TGAAAATTTG 
GGGAATGTGA TTTTGAAGAG AATACTGTTG CTTGTGCTTA TACTTGATAA AGCTAAGTGT 
CAAAGTTGCC TCCCGATTGA GTATGGTATT GACGGACTGG ACAGTGGTTC CCCTTTATTA 
TTCAAAGCAG AATCTTGGGT TAAAGCAAGT AGTCAAGTGA TCCAGGAATT TCTGTCATCT 
GATGTAATGC GCGGAGAAGG CAATCTTCTA ACACATCTGG TTATTTTAGG TTACAAACTA 
ACTCACCAAC AGGGACCTCT TGTTGAGTAT GATTTCCGAG TCAAGGATTT ATTTATAGAT 
CTCCAAGATG GGCTAAAATT GTGTAGAGCT ATTCATCTCT TGCAAAATGA TTCTTCTATA 
CTCAAGAAAA TTGCAGTTCC TTCTGACACC CGTAAGAAAA ATATGGTAAA CAGTGGTGTT 
GCCCTACAAT ATCTTAGACT AGCCGGGGTC TCACTACTTG ATGAAGACGG CACAATGATT 
GTAGCAGATG ATATTGTTAA TGGAGACAGA GAACTCACAA TTTCTTTACT CTGGAATATG 
TTCATTCACT TGCAGCTACC TTTGTTGGTT GATAAAACAA GTTTAGTTGG GGAAATTTCA 
AAAATTCGCG GACTTGGCAC GGAACTCATG ACCGGTGCAA ATTCTAGTTC TCTGGAATTG 
CTTTTGAAAT GGATTCAGGC TGTTTGTGAC CATTATAACT GCCCAGTTGA CAATTTTCAC 
TCCCTTGTTG ATGGCAAAGC AATCTGGTGC TTACTTGATC ATTACTTCCA GAAGGAACTT 
CACAATGTTT GTTCGTTGAA GGAATTTTAT GAAAAAAGTG GCAAGACATC AATTATGTCA 
GTGAATGAGT ATTCAGATGC ACTGTACAAT TTCATATTGT CCCAGAAATT GACCACATTA 
CTGGGGAATT TTCCTGAGGT ACTTCAGATT AGTGAGCTAC TTCAATATAA TGGTGCTTGC 
AGCGATCGAA GTGTGGTGAT ATTGGTTGTT TTCCTGGCAA GCCAGTTATT TGTCAAGGAA 
AAAGTGGACA ACTTAAATTT TCATAAGCTA TTAGGTTATG ACTGCCAAAA CACAAATCGC 
AGGAACTTGA GGACTGTACA GTGCCATTCC CGTTCTGAAT CAGCACAAAA ACCTTATGAT 
TCAGATGTGG GTGACAATGA AGATACGGCA AGAAAATTCA AGGCCATTCA GACTTGGTGG 
CAAGATATGG CTGATCGAAA CTGTATTATG CAGCCAACTA TCTCTATTTT GCAGACATCT 
ATGACCACAG AATGCAACAC TTCTGTTAGA AGAGAAAATG CTGCAAGAAC AATACAATCA 
CGTATCCGAG GACTGGTTGT ACACCGCAAG TTTCACAAGA TGGTAAATAG TGTTACCCTT 
TTGCAGACCG TTTTCAGAGC TTGGCTGAAA GTGAGGCAGG AGTCGGTCTG CATTATATTC 
ACTACCGGTC CAATCTATGA TTTCTCATGT GAAATATTGA AGAAATCCGA AGTATACGAG 
AAGTATGTCA TGCTTTTCTA CCAAAGGCAT TCTTTTTTGA GGTTGAAAAG ATCAGCGCAG 
CTTATCCAGC AAGCAGTGAG GAGTTGGTTC TATTGGAGGG CTCGACAAGG ATGTAGAAGT 
CCTGACCTCT TGACTGCAGA CACTGTGAAT GCTGCCACTT CTATTCAGAA ATTCCTTCGT 
GGTTGGATGG CACGATCTAG ATATATTTAT CTGCTAGATC AAAAAGAGAA AACTCTACAT 
TTAGCCGAAC AGAAACTTAT CTTTGATCTT AAAACAAAGG CAGCCATTGG CATTCAGGTT 
GCTTGGAAAA ACTACATACG TTGCAAGTCT ACTAGGAAGG AGCACTTGTT TGCAACTAAA 
ATTCAATGTA ATTTCCGTAG GTGGTTATTG AGAAAACGAT TTATAAATCA GATTCAAGCT 
GTCATAAAGA TTCAATCATA TTTTCGAATG TGGAGATGTG TGATTGCTAT TCAGAACTTC 
AAAACCATGT CTAAGGCTGC TATTGTCATT CAATCTTTTT TCCGTGGATG GATTGCTCGG 
AAGAATGCTT GTGCTCGTAA GAATCAAATT GTTGAGATTC AAAGGCATTG CCGTGGTTGG 
CTTGTGAAAC GGAATTTCTT GTTTCAAAGA GATGCTATAG TAAAGATCCA GAGTGTCAGT 
CGAAGTTTGA AATGCCAGAA GACACTCAAT TGTCAGAAAG ATGCTGCACT GGAGATCCAG 
CGCTTCATCA GGGGACATTT AACCCGAAAT CGGCTATTAG GTAGTGCTTT AAGGTTACTT 
TCAGCTGATA CTGGTAGTTG CATTTCAAGA CCAGCTGGCT TATGTAGTTT TCAACTTGAA 
GCATTCATGT TCGCAGTAGT GAAATTGCAA CGGTGGTGGA GGGGTCTCTT GTTGCTTAAA 
TTAATGACTA GATCGGCCAT CATAATTCAA TCTTGTACTC GTGGGTGGAT AGCCAGACGG 
AAGGCCATAG TTGAGACACA GAATATTAAT GTCATGGAAG AGTATGCCGC ATTGGAGCTC 
CAGCGCTATA TTAGGGGGCA TTTAACTCGA AATTTGATAT TAGGCAGTGC TTCTAAGCTA 
CGTGCAGTTG CTGCTGGATG CATTTCAAAA CGAACTGGCT TTTGTAGTTT TCAGTTGGAA 
CTATTCTTGT TTCAAGTAGT GAAATTGCAA CGATGGTGGA AGCATCTCTT GTTACATAAA 
TTGATGACAA AGTCTGCAAT CATTATACAG TCCCACATTC GTGGGTGGGC AGCTAGGCGA 
AAGGCGGTTG TTTATAGGCA TCATATCGTT GTCATACAGT CCCACTGGAA AGGTTACGTT 
GCACGTCAGC AGTCAACAAA ACAATTAATG GATTTGCGCT CAAGACTTCA AGAGTCTTCC 
AAAAATGTAG ATGACAGCAA GCGTCTCATT AACAGACTCC TGGCAGCGCT TTCAGAGCTG 
CTTAGTATGA AGAGTCTTAG TGACATTCTT CATACTTGTT CAACGTTGGA TTTGGCAACA 
TGGCATTCTC AGAGATGTTG TGAAGAACTT GTGGCTGCTG GGGCCATTGA CACCTTGCTG 
CGGCTTATTC GATTAATCAG CAGGAGTATC CCTGATCAAG AGGTTTTAAA GCATGTTTTG 
TCAACTCTCC GAAATCTTGC TCGCTATCCT CATCTGCTAG AAGTGCTGAT ACAAAGACAG 
GGTTCTATAC AGACAATCGT CTTGGAGTTA CTGAGGAATA AGGAGGAGGG CTTCTTCATT 
GCTTCTGAGC TTTTGAAGAA GATATGCTCA ACTCAAAAAG GCGTTGAGAC CATACTCAAA 
TCTCCTGCCT TTCTAAAGAG ACTGCACAGT CTTGTTGAGG AGCTTACTAG GAAGGCCACT 
TACCAGAAAA GAAATGTTCG GGGTCCTACT CCTAGTTCAA TTGTTATTGT TAGAGAAAAC 
ACAGATAGAA GATTGAAGGA GGTTACTGAG ATTCTGAAGC TACTAGCACA CCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11443135 ( XM_003610366.3 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003610414.2 Medicago truncatula abnormal spindle-like microcephaly-associated protein homolog (LOC11443135), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe185979
Clone ID Related Accession (Same CDS sequence)	XM_003610366.2 , XM_003610366.3
Accession Version	XM_003610366.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	abnormal spindle-like microcephaly-associated protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016410.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_003610366.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG GTAACAACAA CAACGAGCTT CCATGTCCAT CACCTTCACC TTTTCGCTCT 
TCTTCACTTT TCAAAGACAT TTCTAACTTC AAAACCCCCA AACGCGCTTC TTCTTCTTCT 
TCTTTTCTCT CTCAACAACA TACTCCTCAA ACTCAATTTT TCACCGCTTC CAAATCCAAA 
CACACCACAA CACTCCCGAG GAGGCCGAGA CTCAAAGAAA ATCATACCAT TTCAACTGCT 
TCAAAGAAAC TCAAAGCTTT TCAACTCGAA CAATCTCACT CCTCCAGAAT CGAACAAATC 
AAACAACAAC AATCGCTTAA ATCGTTAGCC AAATCACTCA CTGTTTGGCT TAATTTCCTT 
CTCGAATCAC CTGCTTCTTG CGGTTGCGAT GTTTCAATCG CCGGTGGAAG TCAGATCGCA 
GATGCTTCTC CGGTGACGAG TAAGGGGAAA CGTGATAATG TTCCGGGAAA TTCATTTGGA 
GTTGATTCTA CTTGGAGAAC TCCGAAACGA CAGAGGAAGA CGACGATGAC AACTTCTTCG 
TCGCGTTTTG GTAAGGAGAA TGTGTCTGCT GTGGATATGC AGAATTCATC GTTTTCGCGT 
TTGAAGGATT CTTTGAAGGA TGTATGTAGC TTCGATGATT TTAAGCAGCG GATGAGTGTT 
TATTTGAGTC TCGGAACTTG TGAAGATATT TTCCATGTCA TGAATCAAGT CACTAAGACA 
ATTGATGAAG GAAGGCTAAA TATGAAGGCA CATTGCCCGA TCGTGACGGA TTTAGGGTTG 
AAGGATAAGG CCATAAAAGT CCTTATGTGT TATAATCCAA GTTGGCTGCG AATTGGTTTA 
TATATAATTT TCGGTGGGGA CTCTTTAGTG TCAAATGGGG ATGGTGATTC TGACAAAGAT 
GTTACATTTT TAAAAATGGT CATTGATAAA CTGTTTTTTA CACACGAAGG TTTCGCAAAA 
TCATATGCAT ATAACAAGAT GGTTGAAGGA GTTTATAGGT CTGGTTACTA TGAAAATTTG 
GGGAATGTGA TTTTGAAGAG AATACTGTTG CTTGTGCTTA TACTTGATAA AGCTAAGTGT 
CAAAGTTGCC TCCCGATTGA GTATGGTATT GACGGACTGG ACAGTGGTTC CCCTTTATTA 
TTCAAAGCAG AATCTTGGGT TAAAGCAAGT AGTCAAGTGA TCCAGGAATT TCTGTCATCT 
GATGTAATGC GCGGAGAAGG CAATCTTCTA ACACATCTGG TTATTTTAGG TTACAAACTA 
ACTCACCAAC AGGGACCTCT TGTTGAGTAT GATTTCCGAG TCAAGGATTT ATTTATAGAT 
CTCCAAGATG GGCTAAAATT GTGTAGAGCT ATTCATCTCT TGCAAAATGA TTCTTCTATA 
CTCAAGAAAA TTGCAGTTCC TTCTGACACC CGTAAGAAAA ATATGGTAAA CAGTGGTGTT 
GCCCTACAAT ATCTTAGACT AGCCGGGGTC TCACTACTTG ATGAAGACGG CACAATGATT 
GTAGCAGATG ATATTGTTAA TGGAGACAGA GAACTCACAA TTTCTTTACT CTGGAATATG 
TTCATTCACT TGCAGCTACC TTTGTTGGTT GATAAAACAA GTTTAGTTGG GGAAATTTCA 
AAAATTCGCG GACTTGGCAC GGAACTCATG ACCGGTGCAA ATTCTAGTTC TCTGGAATTG 
CTTTTGAAAT GGATTCAGGC TGTTTGTGAC CATTATAACT GCCCAGTTGA CAATTTTCAC 
TCCCTTGTTG ATGGCAAAGC AATCTGGTGC TTACTTGATC ATTACTTCCA GAAGGAACTT 
CACAATGTTT GTTCGTTGAA GGAATTTTAT GAAAAAAGTG GCAAGACATC AATTATGTCA 
GTGAATGAGT ATTCAGATGC ACTGTACAAT TTCATATTGT CCCAGAAATT GACCACATTA 
CTGGGGAATT TTCCTGAGGT ACTTCAGATT AGTGAGCTAC TTCAATATAA TGGTGCTTGC 
AGCGATCGAA GTGTGGTGAT ATTGGTTGTT TTCCTGGCAA GCCAGTTATT TGTCAAGGAA 
AAAGTGGACA ACTTAAATTT TCATAAGCTA TTAGGTTATG ACTGCCAAAA CACAAATCGC 
AGGAACTTGA GGACTGTACA GTGCCATTCC CGTTCTGAAT CAGCACAAAA ACCTTATGAT 
TCAGATGTGG GTGACAATGA AGATACGGCA AGAAAATTCA AGGCCATTCA GACTTGGTGG 
CAAGATATGG CTGATCGAAA CTGTATTATG CAGCCAACTA TCTCTATTTT GCAGACATCT 
ATGACCACAG AATGCAACAC TTCTGTTAGA AGAGAAAATG CTGCAAGAAC AATACAATCA 
CGTATCCGAG GACTGGTTGT ACACCGCAAG TTTCACAAGA TGGTAAATAG TGTTACCCTT 
TTGCAGACCG TTTTCAGAGC TTGGCTGAAA GTGAGGCAGG AGTCGGTCTG CATTATATTC 
ACTACCGGTC CAATCTATGA TTTCTCATGT GAAATATTGA AGAAATCCGA AGTATACGAG 
AAGTATGTCA TGCTTTTCTA CCAAAGGCAT TCTTTTTTGA GGTTGAAAAG ATCAGCGCAG 
CTTATCCAGC AAGCAGTGAG GAGTTGGTTC TATTGGAGGG CTCGACAAGG ATGTAGAAGT 
CCTGACCTCT TGACTGCAGA CACTGTGAAT GCTGCCACTT CTATTCAGAA ATTCCTTCGT 
GGTTGGATGG CACGATCTAG ATATATTTAT CTGCTAGATC AAAAAGAGAA AACTCTACAT 
TTAGCCGAAC AGAAACTTAT CTTTGATCTT AAAACAAAGG CAGCCATTGG CATTCAGGTT 
GCTTGGAAAA ACTACATACG TTGCAAGTCT ACTAGGAAGG AGCACTTGTT TGCAACTAAA 
ATTCAATGTA ATTTCCGTAG GTGGTTATTG AGAAAACGAT TTATAAATCA GATTCAAGCT 
GTCATAAAGA TTCAATCATA TTTTCGAATG TGGAGATGTG TGATTGCTAT TCAGAACTTC 
AAAACCATGT CTAAGGCTGC TATTGTCATT CAATCTTTTT TCCGTGGATG GATTGCTCGG 
AAGAATGCTT GTGCTCGTAA GAATCAAATT GTTGAGATTC AAAGGCATTG CCGTGGTTGG 
CTTGTGAAAC GGAATTTCTT GTTTCAAAGA GATGCTATAG TAAAGATCCA GAGTGTCAGT 
CGAAGTTTGA AATGCCAGAA GACACTCAAT TGTCAGAAAG ATGCTGCACT GGAGATCCAG 
CGCTTCATCA GGGGACATTT AACCCGAAAT CGGCTATTAG GTAGTGCTTT AAGGTTACTT 
TCAGCTGATA CTGGTAGTTG CATTTCAAGA CCAGCTGGCT TATGTAGTTT TCAACTTGAA 
GCATTCATGT TCGCAGTAGT GAAATTGCAA CGGTGGTGGA GGGGTCTCTT GTTGCTTAAA 
TTAATGACTA GATCGGCCAT CATAATTCAA TCTTGTACTC GTGGGTGGAT AGCCAGACGG 
AAGGCCATAG TTGAGACACA GAATATTAAT GTCATGGAAG AGTATGCCGC ATTGGAGCTC 
CAGCGCTATA TTAGGGGGCA TTTAACTCGA AATTTGATAT TAGGCAGTGC TTCTAAGCTA 
CGTGCAGTTG CTGCTGGATG CATTTCAAAA CGAACTGGCT TTTGTAGTTT TCAGTTGGAA 
CTATTCTTGT TTCAAGTAGT GAAATTGCAA CGATGGTGGA AGCATCTCTT GTTACATAAA 
TTGATGACAA AGTCTGCAAT CATTATACAG TCCCACATTC GTGGGTGGGC AGCTAGGCGA 
AAGGCGGTTG TTTATAGGCA TCATATCGTT GTCATACAGT CCCACTGGAA AGGTTACGTT 
GCACGTCAGC AGTCAACAAA ACAATTAATG GATTTGCGCT CAAGACTTCA AGAGTCTTCC 
AAAAATGTAG ATGACAGCAA GCGTCTCATT AACAGACTCC TGGCAGCGCT TTCAGAGCTG 
CTTAGTATGA AGAGTCTTAG TGACATTCTT CATACTTGTT CAACGTTGGA TTTGGCAACA 
TGGCATTCTC AGAGATGTTG TGAAGAACTT GTGGCTGCTG GGGCCATTGA CACCTTGCTG 
CGGCTTATTC GATTAATCAG CAGGAGTATC CCTGATCAAG AGGTTTTAAA GCATGTTTTG 
TCAACTCTCC GAAATCTTGC TCGCTATCCT CATCTGCTAG AAGTGCTGAT ACAAAGACAG 
GGTTCTATAC AGACAATCGT CTTGGAGTTA CTGAGGAATA AGGAGGAGGG CTTCTTCATT 
GCTTCTGAGC TTTTGAAGAA GATATGCTCA ACTCAAAAAG GCGTTGAGAC CATACTCAAA 
TCTCCTGCCT TTCTAAAGAG ACTGCACAGT CTTGTTGAGG AGCTTACTAG GAAGGCCACT 
TACCAGAAAA GAAATGTTCG GGGTCCTACT CCTAGTTCAA TTGTTATTGT TAGAGAAAAC 
ACAGATAGAA GATTGAAGGA GGTTACTGAG ATTCTGAAGC TACTAGCACA CCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003610414.2
CDS	111..4607
Translation	MEEGNNNNELPCPSPSPFRSSSLFKDISNFKTPKRASSSSSFLSQQHTPQTQFFTASKSKHTTTLPRRPRLKENHTISTASKKLKAFQLEQSHSSRIEQIKQQQSLKSLAKSLTVWLNFLLESPASCGCDVSIAGGSQIADASPVTSKGKRDNVPGNSFGVDSTWRTPKRQRKTTMTTSSSRFGKENVSAVDMQNSSFSRLKDSLKDVCSFDDFKQRMSVYLSLGTCEDIFHVMNQVTKTIDEGRLNMKAHCPIVTDLGLKDKAIKVLMCYNPSWLRIGLYIIFGGDSLVSNGDGDSDKDVTFLKMVIDKLFFTHEGFAKSYAYNKMVEGVYRSGYYENLGNVILKRILLLVLILDKAKCQSCLPIEYGIDGLDSGSPLLFKAESWVKASSQVIQEFLSSDVMRGEGNLLTHLVILGYKLTHQQGPLVEYDFRVKDLFIDLQDGLKLCRAIHLLQNDSSILKKIAVPSDTRKKNMVNSGVALQYLRLAGVSLLDEDGTMIVADDIVNGDRELTISLLWNMFIHLQLPLLVDKTSLVGEISKIRGLGTELMTGANSSSLELLLKWIQAVCDHYNCPVDNFHSLVDGKAIWCLLDHYFQKELHNVCSLKEFYEKSGKTSIMSVNEYSDALYNFILSQKLTTLLGNFPEVLQISELLQYNGACSDRSVVILVVFLASQLFVKEKVDNLNFHKLLGYDCQNTNRRNLRTVQCHSRSESAQKPYDSDVGDNEDTARKFKAIQTWWQDMADRNCIMQPTISILQTSMTTECNTSVRRENAARTIQSRIRGLVVHRKFHKMVNSVTLLQTVFRAWLKVRQESVCIIFTTGPIYDFSCEILKKSEVYEKYVMLFYQRHSFLRLKRSAQLIQQAVRSWFYWRARQGCRSPDLLTADTVNAATSIQKFLRGWMARSRYIYLLDQKEKTLHLAEQKLIFDLKTKAAIGIQVAWKNYIRCKSTRKEHLFATKIQCNFRRWLLRKRFINQIQAVIKIQSYFRMWRCVIAIQNFKTMSKAAIVIQSFFRGWIARKNACARKNQIVEIQRHCRGWLVKRNFLFQRDAIVKIQSVSRSLKCQKTLNCQKDAALEIQRFIRGHLTRNRLLGSALRLLSADTGSCISRPAGLCSFQLEAFMFAVVKLQRWWRGLLLLKLMTRSAIIIQSCTRGWIARRKAIVETQNINVMEEYAALELQRYIRGHLTRNLILGSASKLRAVAAGCISKRTGFCSFQLELFLFQVVKLQRWWKHLLLHKLMTKSAIIIQSHIRGWAARRKAVVYRHHIVVIQSHWKGYVARQQSTKQLMDLRSRLQESSKNVDDSKRLINRLLAALSELLSMKSLSDILHTCSTLDLATWHSQRCCEELVAAGAIDTLLRLIRLISRSIPDQEVLKHVLSTLRNLARYPHLLEVLIQRQGSIQTIVLELLRNKEEGFFIASELLKKICSTQKGVETILKSPAFLKRLHSLVEELTRKATYQKRNVRGPTPSSIVIVRENTDRRLKEVTEILKLLAHP

Target ORF information:

RefSeq Version	XM_003610366.3
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula abnormal spindle-like microcephaly-associated protein homolog (LOC11443135), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003610366.3

ORF Insert Sequence:

ATGGAAGAAG GTAACAACAA CAACGAGCTT CCATGTCCAT CACCTTCACC TTTTCGCTCT 
TCTTCACTTT TCAAAGACAT TTCTAACTTC AAAACCCCCA AACGCGCTTC TTCTTCTTCT 
TCTTTTCTCT CTCAACAACA TACTCCTCAA ACTCAATTTT TCACCGCTTC CAAATCCAAA 
CACACCACAA CACTCCCGAG GAGGCCGAGA CTCAAAGAAA ATCATACCAT TTCAACTGCT 
TCAAAGAAAC TCAAAGCTTT TCAACTCGAA CAATCTCACT CCTCCAGAAT CGAACAAATC 
AAACAACAAC AATCGCTTAA ATCGTTAGCC AAATCACTCA CTGTTTGGCT TAATTTCCTT 
CTCGAATCAC CTGCTTCTTG CGGTTGCGAT GTTTCAATCG CCGGTGGAAG TCAGATCGCA 
GATGCTTCTC CGGTGACGAG TAAGGGGAAA CGTGATAATG TTCCGGGAAA TTCATTTGGA 
GTTGATTCTA CTTGGAGAAC TCCGAAACGA CAGAGGAAGA CGACGATGAC AACTTCTTCG 
TCGCGTTTTG GTAAGGAGAA TGTGTCTGCT GTGGATATGC AGAATTCATC GTTTTCGCGT 
TTGAAGGATT CTTTGAAGGA TGTATGTAGC TTCGATGATT TTAAGCAGCG GATGAGTGTT 
TATTTGAGTC TCGGAACTTG TGAAGATATT TTCCATGTCA TGAATCAAGT CACTAAGACA 
ATTGATGAAG GAAGGCTAAA TATGAAGGCA CATTGCCCGA TCGTGACGGA TTTAGGGTTG 
AAGGATAAGG CCATAAAAGT CCTTATGTGT TATAATCCAA GTTGGCTGCG AATTGGTTTA 
TATATAATTT TCGGTGGGGA CTCTTTAGTG TCAAATGGGG ATGGTGATTC TGACAAAGAT 
GTTACATTTT TAAAAATGGT CATTGATAAA CTGTTTTTTA CACACGAAGG TTTCGCAAAA 
TCATATGCAT ATAACAAGAT GGTTGAAGGA GTTTATAGGT CTGGTTACTA TGAAAATTTG 
GGGAATGTGA TTTTGAAGAG AATACTGTTG CTTGTGCTTA TACTTGATAA AGCTAAGTGT 
CAAAGTTGCC TCCCGATTGA GTATGGTATT GACGGACTGG ACAGTGGTTC CCCTTTATTA 
TTCAAAGCAG AATCTTGGGT TAAAGCAAGT AGTCAAGTGA TCCAGGAATT TCTGTCATCT 
GATGTAATGC GCGGAGAAGG CAATCTTCTA ACACATCTGG TTATTTTAGG TTACAAACTA 
ACTCACCAAC AGGGACCTCT TGTTGAGTAT GATTTCCGAG TCAAGGATTT ATTTATAGAT 
CTCCAAGATG GGCTAAAATT GTGTAGAGCT ATTCATCTCT TGCAAAATGA TTCTTCTATA 
CTCAAGAAAA TTGCAGTTCC TTCTGACACC CGTAAGAAAA ATATGGTAAA CAGTGGTGTT 
GCCCTACAAT ATCTTAGACT AGCCGGGGTC TCACTACTTG ATGAAGACGG CACAATGATT 
GTAGCAGATG ATATTGTTAA TGGAGACAGA GAACTCACAA TTTCTTTACT CTGGAATATG 
TTCATTCACT TGCAGCTACC TTTGTTGGTT GATAAAACAA GTTTAGTTGG GGAAATTTCA 
AAAATTCGCG GACTTGGCAC GGAACTCATG ACCGGTGCAA ATTCTAGTTC TCTGGAATTG 
CTTTTGAAAT GGATTCAGGC TGTTTGTGAC CATTATAACT GCCCAGTTGA CAATTTTCAC 
TCCCTTGTTG ATGGCAAAGC AATCTGGTGC TTACTTGATC ATTACTTCCA GAAGGAACTT 
CACAATGTTT GTTCGTTGAA GGAATTTTAT GAAAAAAGTG GCAAGACATC AATTATGTCA 
GTGAATGAGT ATTCAGATGC ACTGTACAAT TTCATATTGT CCCAGAAATT GACCACATTA 
CTGGGGAATT TTCCTGAGGT ACTTCAGATT AGTGAGCTAC TTCAATATAA TGGTGCTTGC 
AGCGATCGAA GTGTGGTGAT ATTGGTTGTT TTCCTGGCAA GCCAGTTATT TGTCAAGGAA 
AAAGTGGACA ACTTAAATTT TCATAAGCTA TTAGGTTATG ACTGCCAAAA CACAAATCGC 
AGGAACTTGA GGACTGTACA GTGCCATTCC CGTTCTGAAT CAGCACAAAA ACCTTATGAT 
TCAGATGTGG GTGACAATGA AGATACGGCA AGAAAATTCA AGGCCATTCA GACTTGGTGG 
CAAGATATGG CTGATCGAAA CTGTATTATG CAGCCAACTA TCTCTATTTT GCAGACATCT 
ATGACCACAG AATGCAACAC TTCTGTTAGA AGAGAAAATG CTGCAAGAAC AATACAATCA 
CGTATCCGAG GACTGGTTGT ACACCGCAAG TTTCACAAGA TGGTAAATAG TGTTACCCTT 
TTGCAGACCG TTTTCAGAGC TTGGCTGAAA GTGAGGCAGG AGTCGGTCTG CATTATATTC 
ACTACCGGTC CAATCTATGA TTTCTCATGT GAAATATTGA AGAAATCCGA AGTATACGAG 
AAGTATGTCA TGCTTTTCTA CCAAAGGCAT TCTTTTTTGA GGTTGAAAAG ATCAGCGCAG 
CTTATCCAGC AAGCAGTGAG GAGTTGGTTC TATTGGAGGG CTCGACAAGG ATGTAGAAGT 
CCTGACCTCT TGACTGCAGA CACTGTGAAT GCTGCCACTT CTATTCAGAA ATTCCTTCGT 
GGTTGGATGG CACGATCTAG ATATATTTAT CTGCTAGATC AAAAAGAGAA AACTCTACAT 
TTAGCCGAAC AGAAACTTAT CTTTGATCTT AAAACAAAGG CAGCCATTGG CATTCAGGTT 
GCTTGGAAAA ACTACATACG TTGCAAGTCT ACTAGGAAGG AGCACTTGTT TGCAACTAAA 
ATTCAATGTA ATTTCCGTAG GTGGTTATTG AGAAAACGAT TTATAAATCA GATTCAAGCT 
GTCATAAAGA TTCAATCATA TTTTCGAATG TGGAGATGTG TGATTGCTAT TCAGAACTTC 
AAAACCATGT CTAAGGCTGC TATTGTCATT CAATCTTTTT TCCGTGGATG GATTGCTCGG 
AAGAATGCTT GTGCTCGTAA GAATCAAATT GTTGAGATTC AAAGGCATTG CCGTGGTTGG 
CTTGTGAAAC GGAATTTCTT GTTTCAAAGA GATGCTATAG TAAAGATCCA GAGTGTCAGT 
CGAAGTTTGA AATGCCAGAA GACACTCAAT TGTCAGAAAG ATGCTGCACT GGAGATCCAG 
CGCTTCATCA GGGGACATTT AACCCGAAAT CGGCTATTAG GTAGTGCTTT AAGGTTACTT 
TCAGCTGATA CTGGTAGTTG CATTTCAAGA CCAGCTGGCT TATGTAGTTT TCAACTTGAA 
GCATTCATGT TCGCAGTAGT GAAATTGCAA CGGTGGTGGA GGGGTCTCTT GTTGCTTAAA 
TTAATGACTA GATCGGCCAT CATAATTCAA TCTTGTACTC GTGGGTGGAT AGCCAGACGG 
AAGGCCATAG TTGAGACACA GAATATTAAT GTCATGGAAG AGTATGCCGC ATTGGAGCTC 
CAGCGCTATA TTAGGGGGCA TTTAACTCGA AATTTGATAT TAGGCAGTGC TTCTAAGCTA 
CGTGCAGTTG CTGCTGGATG CATTTCAAAA CGAACTGGCT TTTGTAGTTT TCAGTTGGAA 
CTATTCTTGT TTCAAGTAGT GAAATTGCAA CGATGGTGGA AGCATCTCTT GTTACATAAA 
TTGATGACAA AGTCTGCAAT CATTATACAG TCCCACATTC GTGGGTGGGC AGCTAGGCGA 
AAGGCGGTTG TTTATAGGCA TCATATCGTT GTCATACAGT CCCACTGGAA AGGTTACGTT 
GCACGTCAGC AGTCAACAAA ACAATTAATG GATTTGCGCT CAAGACTTCA AGAGTCTTCC 
AAAAATGTAG ATGACAGCAA GCGTCTCATT AACAGACTCC TGGCAGCGCT TTCAGAGCTG 
CTTAGTATGA AGAGTCTTAG TGACATTCTT CATACTTGTT CAACGTTGGA TTTGGCAACA 
TGGCATTCTC AGAGATGTTG TGAAGAACTT GTGGCTGCTG GGGCCATTGA CACCTTGCTG 
CGGCTTATTC GATTAATCAG CAGGAGTATC CCTGATCAAG AGGTTTTAAA GCATGTTTTG 
TCAACTCTCC GAAATCTTGC TCGCTATCCT CATCTGCTAG AAGTGCTGAT ACAAAGACAG 
GGTTCTATAC AGACAATCGT CTTGGAGTTA CTGAGGAATA AGGAGGAGGG CTTCTTCATT 
GCTTCTGAGC TTTTGAAGAA GATATGCTCA ACTCAAAAAG GCGTTGAGAC CATACTCAAA 
TCTCCTGCCT TTCTAAAGAG ACTGCACAGT CTTGTTGAGG AGCTTACTAG GAAGGCCACT 
TACCAGAAAA GAAATGTTCG GGGTCCTACT CCTAGTTCAA TTGTTATTGT TAGAGAAAAC 
ACAGATAGAA GATTGAAGGA GGTTACTGAG ATTCTGAAGC TACTAGCACA CCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11443135 ( XM_024782542.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024638310.1 Medicago truncatula abnormal spindle-like microcephaly-associated protein homolog (LOC11443135), transcript variant X1, mRNA.

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CloneID	OMe264161
Clone ID Related Accession (Same CDS sequence)	XM_024782542.1
Accession Version	XM_024782542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	abnormal spindle-like microcephaly-associated protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016410.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG GTAACAACAA CAACGAGCTT CCATGTCCAT CACCTTCACC TTTTCGCTCT 
TCTTCACTTT TCAAAGACAT TTCTAACTTC AAAACCCCCA AACGCGCTTC TTCTTCTTCT 
TCTTTTCTCT CTCAACAACA TACTCCTCAA ACTCAATTTT TCACCGCTTC CAAATCCAAA 
CACACCACAA CACTCCCGAG GAGGCCGAGA CTCAAAGAAA ATCATACCAT TTCAACTGCT 
TCAAAGAAAC TCAAAGCTTT TCAACTCGAA CAATCTCACT CCTCCAGAAT CGAACAAATC 
AAACAACAAC AATCGCTTAA ATCGTTAGCC AAATCACTCA CTGTTTGGCT TAATTTCCTT 
CTCGAATCAC CTGCTTCTTG CGGTTGCGAT GTTTCAATCG CCGGTGGAAG TCAGATCGCA 
GATGCTTCTC CGGTGACGAG TAAGGGGAAA CGTGATAATG TTCCGGGAAA TTCATTTGGA 
GTTGATTCTA CTTGGAGAAC TCCGAAACGA CAGAGGAAGA CGACGATGAC AACTTCTTCG 
TCGCGTTTTG GTAAGGAGAA TGTGTCTGCT GTGGATATGC AGAATTCATC GTTTTCGCGT 
TTGAAGGATT CTTTGAAGGA TGTATGTAGC TTCGATGATT TTAAGCAGCG GATGAGTGTT 
TATTTGAGTC TCGGAACTTG TGAAGATATT TTCCATGTCA TGAATCAAGT CACTAAGACA 
ATTGATGAAG GAAGGCTAAA TATGAAGGCA CATTGCCCGA TCGTGACGGA TTTAGGGTTG 
AAGGATAAGG CCATAAAAGT CCTTATGTGT TATAATCCAA GTTGGCTGCG AATTGGTTTA 
TATATAATTT TCGGTGGGGA CTCTTTAGTG TCAAATGGGG ATGGTGATTC TGACAAAGAT 
GTTACATTTT TAAAAATGGT CATTGATAAA CTGTTTTTTA CACACGAAGG TTTCGCAAAA 
TCATATGCAT ATAACAAGAT GGTTGAAGGA GTTTATAGGT CTGGTTACTA TGAAAATTTG 
GGGAATGTGA TTTTGAAGAG AATACTGTTG CTTGTGCTTA TACTTGATAA AGCTAAGTGT 
CAAAGTTGCC TCCCGATTGA GTATGGTATT GACGGACTGG ACAGTGGTTC CCCTTTATTA 
TTCAAAGCAG AATCTTGGGT TAAAGCAAGT AGTCAAGTGA TCCAGGAATT TCTGTCATCT 
GATGTAATGC GCGGAGAAGG CAATCTTCTA ACACATCTGG TTATTTTAGG TTACAAACTA 
ACTCACCAAC AGGGACCTCT TGTTGAGTAT GATTTCCGAG TCAAGGATTT ATTTATAGAT 
CTCCAAGATG GGCTAAAATT GTGTAGAGCT ATTCATCTCT TGCAAAATGA TTCTTCTATA 
CTCAAGAAAA TTGCAGTTCC TTCTGACACC CGTAAGAAAA ATATGGTAAA CAGTGGTGTT 
GCCCTACAAT ATCTTAGACT AGCCGGGGTC TCACTACTTG ATGAAGACGG CACAATGATT 
GTAGCAGATG ATATTGTTAA TGGAGACAGA GAACTCACAA TTTCTTTACT CTGGAATATG 
TTCATTCACT TGCAGCTACC TTTGTTGGTT GATAAAACAA GTTTAGTTGG GGAAATTTCA 
AAAATTCGCG GACTTGGCAC GGAACTCATG ACCGGTGCAA ATTCTAGTTC TCTGGAATTG 
CTTTTGAAAT GGATTCAGGC TGTTTGTGAC CATTATAACT GCCCAGTTGA CAATTTTCAC 
TCCCTTGTTG ATGGCAAAGC AATCTGGTGC TTACTTGATC ATTACTTCCA GAAGGAACTT 
CACAATGTTT GTTCGTTGAA GGAATTTTAT GAAAAAAGTG GCAAGACATC AATTATGTCA 
GTGAATGAGT ATTCAGATGC ACTGTACAAT TTCATATTGT CCCAGAAATT GACCACATTA 
CTGGGGAATT TTCCTGAGGT ACTTCAGATT AGTGAGCTAC TTCAATATAA TGGTGCTTGC 
AGCGATCGAA GTGTGGTGAT ATTGGTTGTT TTCCTGGCAA GCCAGTTATT TGTCAAGGAA 
AAAGTGGACA ACTTAAATTT TCATAAGCTA TTAGGTTATG ACTGCCAAAA CACAAATCGC 
AGGAACTTGA GGACTGTACA GTGCCATTCC CGTTCTGAAT CAGCACAAAA ACCTTATGAT 
TCAGATGTGG GTGACAATGA AGATACGGCA AGAAAATTCA AGGCCATTCA GACTTGGTGG 
CAAGATATGG CTGATCGAAA CTGTATTATG CAGCCAACTA TCTCTATTTT GCAGACATCT 
ATGACCACAG AATGCAACAC TTCTGTTAGA AGAGAAAATG CTGCAAGAAC AATACAATCA 
CGTATCCGAG GACTGGTTGT ACACCGCAAG TTTCACAAGA TGGTAAATAG TGTTACCCTT 
TTGCAGACCG TTTTCAGAGC TTGGCTGAAA GTGAGGCAGG AGTCGGTCTG CATTATATTC 
ACTACCGGTC CAATCTATGA TTTCTCATGT GAAATATTGA AGAAATCCGA AGTATACGAG 
AAGTATGTCA TGCTTTTCTA CCAAAGGCAT TCTTTTTTGA GGTTGAAAAG ATCAGCGCAG 
CTTATCCAGC AAGCAGTGAG GAGTTGGTTC TATTGGAGGG CTCGACAAGG ATGTAGAAGT 
CCTGACCTCT TGACTGCAGA CACTGTGAAT GCTGCCACTT CTATTCAGAA ATTCCTTCGT 
GGTTGGATGG CACGATCTAG ATATATTTAT CTGCTAGATC AAAAAGAGAA AACTCTACAT 
TTAGCCGAAC AGAAACTTAT CTTTGATCTT AAAACAAAGG CAGCCATTGG CATTCAGGTT 
GCTTGGAAAA ACTACATACG TTGCAAGTCT ACTAGGAAGG AGCACTTGTT TGCAACTAAA 
ATTCAATGTA ATTTCCGTAG GTGGTTATTG AGAAAACGAT TTATAAATCA GATTCAAGCT 
GTCATAAAGA TTCAATCATA TTTTCGAATG TGGAGATGTG TGATTGCTAT TCAGAACTTC 
AAAACCATGT CTAAGGCTGC TATTGTCATT CAATCTTTTT TCCGTGGATG GATTGCTCGG 
AAGAATGCTT GTGCTCGTAA GAATCAAATT GTTGAGATTC AAAGGCATTG CCGTGGTTGG 
CTTGTGAAAC GGAATTTCTT GTTTCAAAGA GATGCTATAG TAAAGATCCA GAGTGTCAGT 
CGAAGTTTGA AATGCCAGAA GACACTCAAT TGTCAGAAAG ATGCTGCACT GGAGATCCAG 
CGCTTCATCA GGGGACATTT AACCCGAAAT CGGCTATTAG GTAGTGCTTT AAGGTTACTT 
TCAGCTGATA CTGGTAGTTG CATTTCAAGA CCAGCTGGCT TATGTAGTTT TCAACTTGAA 
GCATTCATGT TCGCAGTAGT GAAATTGCAA CGGTGGTGGA GGGGTCTCTT GTTGCTTAAA 
TTAATGACTA GATCGGCCAT CATAATTCAA TCTTGTACTC GTGGGTGGAT AGCCAGACGG 
AAGGCCATAG TTGAGACACA GAATATTAAT GTCATGGAAG TTCGTAACTG CCAGGAGTAT 
GCCGCATTGG AGCTCCAGCG CTATATTAGG GGGCATTTAA CTCGAAATTT GATATTAGGC 
AGTGCTTCTA AGCTACGTGC AGTTGCTGCT GGATGCATTT CAAAACGAAC TGGCTTTTGT 
AGTTTTCAGT TGGAACTATT CTTGTTTCAA GTAGTGAAAT TGCAACGATG GTGGAAGCAT 
CTCTTGTTAC ATAAATTGAT GACAAAGTCT GCAATCATTA TACAGTCCCA CATTCGTGGG 
TGGGCAGCTA GGCGAAAGGC GGTTGTTTAT AGGCATCATA TCGTTGTCAT ACAGTCCCAC 
TGGAAAGGTT ACGTTGCACG TCAGCAGTCA ACAAAACAAT TAATGGATTT GCGCTCAAGA 
CTTCAAGAGT CTTCCAAAAA TGTAGATGAC AGCAAGCGTC TCATTAACAG ACTCCTGGCA 
GCGCTTTCAG AGCTGCTTAG TATGAAGAGT CTTAGTGACA TTCTTCATAC TTGTTCAACG 
TTGGATTTGG CAACATGGCA TTCTCAGAGA TGTTGTGAAG AACTTGTGGC TGCTGGGGCC 
ATTGACACCT TGCTGCGGCT TATTCGATTA ATCAGCAGGA GTATCCCTGA TCAAGAGGTT 
TTAAAGCATG TTTTGTCAAC TCTCCGAAAT CTTGCTCGCT ATCCTCATCT GCTAGAAGTG 
CTGATACAAA GACAGGGTTC TATACAGACA ATCGTCTTGG AGTTACTGAG GAATAAGGAG 
GAGGGCTTCT TCATTGCTTC TGAGCTTTTG AAGAAGATAT GCTCAACTCA AAAAGGCGTT 
GAGACCATAC TCAAATCTCC TGCCTTTCTA AAGAGACTGC ACAGTCTTGT TGAGGAGCTT 
ACTAGGAAGG CCACTTACCA GAAAAGAAAT GTTCGGGGTC CTACTCCTAG TTCAATTGTT 
ATTGTTAGAG AAAACACAGA TAGAAGATTG AAGGAGGTTA CTGAGATTCT GAAGCTACTA 
GCACACCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024638310.1
CDS	111..4622
Translation	MEEGNNNNELPCPSPSPFRSSSLFKDISNFKTPKRASSSSSFLSQQHTPQTQFFTASKSKHTTTLPRRPRLKENHTISTASKKLKAFQLEQSHSSRIEQIKQQQSLKSLAKSLTVWLNFLLESPASCGCDVSIAGGSQIADASPVTSKGKRDNVPGNSFGVDSTWRTPKRQRKTTMTTSSSRFGKENVSAVDMQNSSFSRLKDSLKDVCSFDDFKQRMSVYLSLGTCEDIFHVMNQVTKTIDEGRLNMKAHCPIVTDLGLKDKAIKVLMCYNPSWLRIGLYIIFGGDSLVSNGDGDSDKDVTFLKMVIDKLFFTHEGFAKSYAYNKMVEGVYRSGYYENLGNVILKRILLLVLILDKAKCQSCLPIEYGIDGLDSGSPLLFKAESWVKASSQVIQEFLSSDVMRGEGNLLTHLVILGYKLTHQQGPLVEYDFRVKDLFIDLQDGLKLCRAIHLLQNDSSILKKIAVPSDTRKKNMVNSGVALQYLRLAGVSLLDEDGTMIVADDIVNGDRELTISLLWNMFIHLQLPLLVDKTSLVGEISKIRGLGTELMTGANSSSLELLLKWIQAVCDHYNCPVDNFHSLVDGKAIWCLLDHYFQKELHNVCSLKEFYEKSGKTSIMSVNEYSDALYNFILSQKLTTLLGNFPEVLQISELLQYNGACSDRSVVILVVFLASQLFVKEKVDNLNFHKLLGYDCQNTNRRNLRTVQCHSRSESAQKPYDSDVGDNEDTARKFKAIQTWWQDMADRNCIMQPTISILQTSMTTECNTSVRRENAARTIQSRIRGLVVHRKFHKMVNSVTLLQTVFRAWLKVRQESVCIIFTTGPIYDFSCEILKKSEVYEKYVMLFYQRHSFLRLKRSAQLIQQAVRSWFYWRARQGCRSPDLLTADTVNAATSIQKFLRGWMARSRYIYLLDQKEKTLHLAEQKLIFDLKTKAAIGIQVAWKNYIRCKSTRKEHLFATKIQCNFRRWLLRKRFINQIQAVIKIQSYFRMWRCVIAIQNFKTMSKAAIVIQSFFRGWIARKNACARKNQIVEIQRHCRGWLVKRNFLFQRDAIVKIQSVSRSLKCQKTLNCQKDAALEIQRFIRGHLTRNRLLGSALRLLSADTGSCISRPAGLCSFQLEAFMFAVVKLQRWWRGLLLLKLMTRSAIIIQSCTRGWIARRKAIVETQNINVMEVRNCQEYAALELQRYIRGHLTRNLILGSASKLRAVAAGCISKRTGFCSFQLELFLFQVVKLQRWWKHLLLHKLMTKSAIIIQSHIRGWAARRKAVVYRHHIVVIQSHWKGYVARQQSTKQLMDLRSRLQESSKNVDDSKRLINRLLAALSELLSMKSLSDILHTCSTLDLATWHSQRCCEELVAAGAIDTLLRLIRLISRSIPDQEVLKHVLSTLRNLARYPHLLEVLIQRQGSIQTIVLELLRNKEEGFFIASELLKKICSTQKGVETILKSPAFLKRLHSLVEELTRKATYQKRNVRGPTPSSIVIVRENTDRRLKEVTEILKLLAHP

Target ORF information:

RefSeq Version	XM_024782542.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula abnormal spindle-like microcephaly-associated protein homolog (LOC11443135), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024782542.1

ORF Insert Sequence:

ATGGAAGAAG GTAACAACAA CAACGAGCTT CCATGTCCAT CACCTTCACC TTTTCGCTCT 
TCTTCACTTT TCAAAGACAT TTCTAACTTC AAAACCCCCA AACGCGCTTC TTCTTCTTCT 
TCTTTTCTCT CTCAACAACA TACTCCTCAA ACTCAATTTT TCACCGCTTC CAAATCCAAA 
CACACCACAA CACTCCCGAG GAGGCCGAGA CTCAAAGAAA ATCATACCAT TTCAACTGCT 
TCAAAGAAAC TCAAAGCTTT TCAACTCGAA CAATCTCACT CCTCCAGAAT CGAACAAATC 
AAACAACAAC AATCGCTTAA ATCGTTAGCC AAATCACTCA CTGTTTGGCT TAATTTCCTT 
CTCGAATCAC CTGCTTCTTG CGGTTGCGAT GTTTCAATCG CCGGTGGAAG TCAGATCGCA 
GATGCTTCTC CGGTGACGAG TAAGGGGAAA CGTGATAATG TTCCGGGAAA TTCATTTGGA 
GTTGATTCTA CTTGGAGAAC TCCGAAACGA CAGAGGAAGA CGACGATGAC AACTTCTTCG 
TCGCGTTTTG GTAAGGAGAA TGTGTCTGCT GTGGATATGC AGAATTCATC GTTTTCGCGT 
TTGAAGGATT CTTTGAAGGA TGTATGTAGC TTCGATGATT TTAAGCAGCG GATGAGTGTT 
TATTTGAGTC TCGGAACTTG TGAAGATATT TTCCATGTCA TGAATCAAGT CACTAAGACA 
ATTGATGAAG GAAGGCTAAA TATGAAGGCA CATTGCCCGA TCGTGACGGA TTTAGGGTTG 
AAGGATAAGG CCATAAAAGT CCTTATGTGT TATAATCCAA GTTGGCTGCG AATTGGTTTA 
TATATAATTT TCGGTGGGGA CTCTTTAGTG TCAAATGGGG ATGGTGATTC TGACAAAGAT 
GTTACATTTT TAAAAATGGT CATTGATAAA CTGTTTTTTA CACACGAAGG TTTCGCAAAA 
TCATATGCAT ATAACAAGAT GGTTGAAGGA GTTTATAGGT CTGGTTACTA TGAAAATTTG 
GGGAATGTGA TTTTGAAGAG AATACTGTTG CTTGTGCTTA TACTTGATAA AGCTAAGTGT 
CAAAGTTGCC TCCCGATTGA GTATGGTATT GACGGACTGG ACAGTGGTTC CCCTTTATTA 
TTCAAAGCAG AATCTTGGGT TAAAGCAAGT AGTCAAGTGA TCCAGGAATT TCTGTCATCT 
GATGTAATGC GCGGAGAAGG CAATCTTCTA ACACATCTGG TTATTTTAGG TTACAAACTA 
ACTCACCAAC AGGGACCTCT TGTTGAGTAT GATTTCCGAG TCAAGGATTT ATTTATAGAT 
CTCCAAGATG GGCTAAAATT GTGTAGAGCT ATTCATCTCT TGCAAAATGA TTCTTCTATA 
CTCAAGAAAA TTGCAGTTCC TTCTGACACC CGTAAGAAAA ATATGGTAAA CAGTGGTGTT 
GCCCTACAAT ATCTTAGACT AGCCGGGGTC TCACTACTTG ATGAAGACGG CACAATGATT 
GTAGCAGATG ATATTGTTAA TGGAGACAGA GAACTCACAA TTTCTTTACT CTGGAATATG 
TTCATTCACT TGCAGCTACC TTTGTTGGTT GATAAAACAA GTTTAGTTGG GGAAATTTCA 
AAAATTCGCG GACTTGGCAC GGAACTCATG ACCGGTGCAA ATTCTAGTTC TCTGGAATTG 
CTTTTGAAAT GGATTCAGGC TGTTTGTGAC CATTATAACT GCCCAGTTGA CAATTTTCAC 
TCCCTTGTTG ATGGCAAAGC AATCTGGTGC TTACTTGATC ATTACTTCCA GAAGGAACTT 
CACAATGTTT GTTCGTTGAA GGAATTTTAT GAAAAAAGTG GCAAGACATC AATTATGTCA 
GTGAATGAGT ATTCAGATGC ACTGTACAAT TTCATATTGT CCCAGAAATT GACCACATTA 
CTGGGGAATT TTCCTGAGGT ACTTCAGATT AGTGAGCTAC TTCAATATAA TGGTGCTTGC 
AGCGATCGAA GTGTGGTGAT ATTGGTTGTT TTCCTGGCAA GCCAGTTATT TGTCAAGGAA 
AAAGTGGACA ACTTAAATTT TCATAAGCTA TTAGGTTATG ACTGCCAAAA CACAAATCGC 
AGGAACTTGA GGACTGTACA GTGCCATTCC CGTTCTGAAT CAGCACAAAA ACCTTATGAT 
TCAGATGTGG GTGACAATGA AGATACGGCA AGAAAATTCA AGGCCATTCA GACTTGGTGG 
CAAGATATGG CTGATCGAAA CTGTATTATG CAGCCAACTA TCTCTATTTT GCAGACATCT 
ATGACCACAG AATGCAACAC TTCTGTTAGA AGAGAAAATG CTGCAAGAAC AATACAATCA 
CGTATCCGAG GACTGGTTGT ACACCGCAAG TTTCACAAGA TGGTAAATAG TGTTACCCTT 
TTGCAGACCG TTTTCAGAGC TTGGCTGAAA GTGAGGCAGG AGTCGGTCTG CATTATATTC 
ACTACCGGTC CAATCTATGA TTTCTCATGT GAAATATTGA AGAAATCCGA AGTATACGAG 
AAGTATGTCA TGCTTTTCTA CCAAAGGCAT TCTTTTTTGA GGTTGAAAAG ATCAGCGCAG 
CTTATCCAGC AAGCAGTGAG GAGTTGGTTC TATTGGAGGG CTCGACAAGG ATGTAGAAGT 
CCTGACCTCT TGACTGCAGA CACTGTGAAT GCTGCCACTT CTATTCAGAA ATTCCTTCGT 
GGTTGGATGG CACGATCTAG ATATATTTAT CTGCTAGATC AAAAAGAGAA AACTCTACAT 
TTAGCCGAAC AGAAACTTAT CTTTGATCTT AAAACAAAGG CAGCCATTGG CATTCAGGTT 
GCTTGGAAAA ACTACATACG TTGCAAGTCT ACTAGGAAGG AGCACTTGTT TGCAACTAAA 
ATTCAATGTA ATTTCCGTAG GTGGTTATTG AGAAAACGAT TTATAAATCA GATTCAAGCT 
GTCATAAAGA TTCAATCATA TTTTCGAATG TGGAGATGTG TGATTGCTAT TCAGAACTTC 
AAAACCATGT CTAAGGCTGC TATTGTCATT CAATCTTTTT TCCGTGGATG GATTGCTCGG 
AAGAATGCTT GTGCTCGTAA GAATCAAATT GTTGAGATTC AAAGGCATTG CCGTGGTTGG 
CTTGTGAAAC GGAATTTCTT GTTTCAAAGA GATGCTATAG TAAAGATCCA GAGTGTCAGT 
CGAAGTTTGA AATGCCAGAA GACACTCAAT TGTCAGAAAG ATGCTGCACT GGAGATCCAG 
CGCTTCATCA GGGGACATTT AACCCGAAAT CGGCTATTAG GTAGTGCTTT AAGGTTACTT 
TCAGCTGATA CTGGTAGTTG CATTTCAAGA CCAGCTGGCT TATGTAGTTT TCAACTTGAA 
GCATTCATGT TCGCAGTAGT GAAATTGCAA CGGTGGTGGA GGGGTCTCTT GTTGCTTAAA 
TTAATGACTA GATCGGCCAT CATAATTCAA TCTTGTACTC GTGGGTGGAT AGCCAGACGG 
AAGGCCATAG TTGAGACACA GAATATTAAT GTCATGGAAG TTCGTAACTG CCAGGAGTAT 
GCCGCATTGG AGCTCCAGCG CTATATTAGG GGGCATTTAA CTCGAAATTT GATATTAGGC 
AGTGCTTCTA AGCTACGTGC AGTTGCTGCT GGATGCATTT CAAAACGAAC TGGCTTTTGT 
AGTTTTCAGT TGGAACTATT CTTGTTTCAA GTAGTGAAAT TGCAACGATG GTGGAAGCAT 
CTCTTGTTAC ATAAATTGAT GACAAAGTCT GCAATCATTA TACAGTCCCA CATTCGTGGG 
TGGGCAGCTA GGCGAAAGGC GGTTGTTTAT AGGCATCATA TCGTTGTCAT ACAGTCCCAC 
TGGAAAGGTT ACGTTGCACG TCAGCAGTCA ACAAAACAAT TAATGGATTT GCGCTCAAGA 
CTTCAAGAGT CTTCCAAAAA TGTAGATGAC AGCAAGCGTC TCATTAACAG ACTCCTGGCA 
GCGCTTTCAG AGCTGCTTAG TATGAAGAGT CTTAGTGACA TTCTTCATAC TTGTTCAACG 
TTGGATTTGG CAACATGGCA TTCTCAGAGA TGTTGTGAAG AACTTGTGGC TGCTGGGGCC 
ATTGACACCT TGCTGCGGCT TATTCGATTA ATCAGCAGGA GTATCCCTGA TCAAGAGGTT 
TTAAAGCATG TTTTGTCAAC TCTCCGAAAT CTTGCTCGCT ATCCTCATCT GCTAGAAGTG 
CTGATACAAA GACAGGGTTC TATACAGACA ATCGTCTTGG AGTTACTGAG GAATAAGGAG 
GAGGGCTTCT TCATTGCTTC TGAGCTTTTG AAGAAGATAT GCTCAACTCA AAAAGGCGTT 
GAGACCATAC TCAAATCTCC TGCCTTTCTA AAGAGACTGC ACAGTCTTGT TGAGGAGCTT 
ACTAGGAAGG CCACTTACCA GAAAAGAAAT GTTCGGGGTC CTACTCCTAG TTCAATTGTT 
ATTGTTAGAG AAAACACAGA TAGAAGATTG AAGGAGGTTA CTGAGATTCT GAAGCTACTA 
GCACACCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC11443135 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム