NUF2 cDNA ORF clone, Leptosomus discolor(cuckoo roller)

Gene
Clones
gRNAs

Summary

Gene Symbol	NUF2
Entrez Gene ID	104352814
Full Name	NUF2, NDC80 kinetochore complex component
Gene Type	protein-coding
Organism	Leptosomus discolor(cuckoo roller)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009959233.1	XP_009957535.1	kinetochore protein Nuf2

Pathways from BioSystems
Gene Ontology

The following NUF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLe127081	XM_009959233.1 Latest version!	Leptosomus discolor NUF2, NDC80 kinetochore complex component (NUF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUF2 ( XM_009959233.1 ) cDNA ORF clone, Leptosomus discolor(cuckoo roller) -> XP_009957535.1 Leptosomus discolor NUF2, NDC80 kinetochore complex component (NUF2), mRNA.

Price & Availability↑

CloneID	OLe127081
Clone ID Related Accession (Same CDS sequence)	XM_009959233.1
Accession Version	XM_009959233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-27
Organism	Leptosomus discolor(cuckoo roller)
Product	kinetochore protein Nuf2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009883543.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Leptosomus discolor Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTACA TGAGAATCCT GCAGAAGGTA TATGGAATCC GCCTGGAGCA TTTCTACATG 
ATGCCAGTCA ACGTCGACAT AATGTATCCA CAGATATTTG AAGGCTTTCT ACCTGTTTGT 
AATTTGTATA TTCACATGGA ACGCTTACTT CCAGTGTGCC GGATTAATGA TTTTCAAATT 
GCTGATGTTT TAAACCCAAA AACAAAGAGG ACAGTTCGCT TCCTGAGTGG CATTCTCAAC 
TTTGTAAATT TCAGAGAATT CCGACGTGAG GTCTACCTGG AGTTACAACT AAACTATAAA 
TCAGCCATGG AGAAACACCA ACAACTGGAA ACATCAAATC GTGAGGCAGC ACTGAAGCTG 
GAGAAACTGA ATACTGTTCC AGTTGAACAC CAAGCAGAGG TCAAGCAACT GACAGACAAC 
ATTCGAGAAC TGGAGCAACT ACTAAGGCAA GACTATCGTC GGAAACAAAC AGCTTTGCAA 
GAAGTGATTT CGCAGAAGAA GTCAGATATT GCGGAGAGTA CCCGAAAACT GAACGAACTT 
AAAGTGACAA TGGCTACTTT GAAAGAAGAA CAAGAGCAGC TGAAATCAAA AATTGTGGAG 
AGTCCAGAGG AACTGAAAAA TTACAAAGAA CTGATGAAAG AAACTGTTAA GAAACTCAAG 
AAATCCAAGC AAGAAGTTAT TGAGAAATAT GAAGGTTATA GAGACCTAGT TGAGGTTCTG 
CCGTCGTGCC AAATGGAGGT GCAGTCATAT CAAAAGAAGA TGGAAAGACA GGGAGCAAAT 
GTGGAGAGAC TGGCTAGTGT ATTATCAGAG GTCAGAAATC TGGAGGACCA GCTAGAGAGT 
GCTCAGATAG AGCTGAAAAA GGGGAAGACA GATGAAATGT CCTTAAAGAG ATTGGTCACT 
GCAAAACATG AGAAGCTGTC TACAGCTGAA ATAAGGATAA AAAAGAAGCG TGAAGATGTT 
GAGCAATATA AGCACACTAT ATTTGAATAT TGTAACAGAG TTCAGGAGAA GAGGGGTGCT 
GTGTATGATA AGGTGACGGC CATTCACAAG GAAATCCAAC AGACAAGATT CAAAATTCAG 
CAGCTGAATG AGAATGCTGA AAAAGAAGAC ATGAAAGCCA AGGAAATATA CCTAAATCTG 
AAGGCTGGGT TGGAGAAGCA TCATGACTCT CTCATTAAGA CAGCAAAGAG TTACGCAGCC 
TCAAGAGAAG ATAAAATTGC TGAACTGAAG AAGGGGCTGC TTAGAGTTCA GTCTCCCGGA 
AATAGTTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009957535.1
CDS	12..1283
Translation	MIYMRILQKVYGIRLEHFYMMPVNVDIMYPQIFEGFLPVCNLYIHMERLLPVCRINDFQIADVLNPKTKRTVRFLSGILNFVNFREFRREVYLELQLNYKSAMEKHQQLETSNREAALKLEKLNTVPVEHQAEVKQLTDNIRELEQLLRQDYRRKQTALQEVISQKKSDIAESTRKLNELKVTMATLKEEQEQLKSKIVESPEELKNYKELMKETVKKLKKSKQEVIEKYEGYRDLVEVLPSCQMEVQSYQKKMERQGANVERLASVLSEVRNLEDQLESAQIELKKGKTDEMSLKRLVTAKHEKLSTAEIRIKKKREDVEQYKHTIFEYCNRVQEKRGAVYDKVTAIHKEIQQTRFKIQQLNENAEKEDMKAKEIYLNLKAGLEKHHDSLIKTAKSYAASREDKIAELKKGLLRVQSPGNSS

Target ORF information:

RefSeq Version	XM_009959233.1
Organism	Leptosomus discolor(cuckoo roller)
Definition	Leptosomus discolor NUF2, NDC80 kinetochore complex component (NUF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009959233.1

ORF Insert Sequence:

ATGATTTACA TGAGAATCCT GCAGAAGGTA TATGGAATCC GCCTGGAGCA TTTCTACATG 
ATGCCAGTCA ACGTCGACAT AATGTATCCA CAGATATTTG AAGGCTTTCT ACCTGTTTGT 
AATTTGTATA TTCACATGGA ACGCTTACTT CCAGTGTGCC GGATTAATGA TTTTCAAATT 
GCTGATGTTT TAAACCCAAA AACAAAGAGG ACAGTTCGCT TCCTGAGTGG CATTCTCAAC 
TTTGTAAATT TCAGAGAATT CCGACGTGAG GTCTACCTGG AGTTACAACT AAACTATAAA 
TCAGCCATGG AGAAACACCA ACAACTGGAA ACATCAAATC GTGAGGCAGC ACTGAAGCTG 
GAGAAACTGA ATACTGTTCC AGTTGAACAC CAAGCAGAGG TCAAGCAACT GACAGACAAC 
ATTCGAGAAC TGGAGCAACT ACTAAGGCAA GACTATCGTC GGAAACAAAC AGCTTTGCAA 
GAAGTGATTT CGCAGAAGAA GTCAGATATT GCGGAGAGTA CCCGAAAACT GAACGAACTT 
AAAGTGACAA TGGCTACTTT GAAAGAAGAA CAAGAGCAGC TGAAATCAAA AATTGTGGAG 
AGTCCAGAGG AACTGAAAAA TTACAAAGAA CTGATGAAAG AAACTGTTAA GAAACTCAAG 
AAATCCAAGC AAGAAGTTAT TGAGAAATAT GAAGGTTATA GAGACCTAGT TGAGGTTCTG 
CCGTCGTGCC AAATGGAGGT GCAGTCATAT CAAAAGAAGA TGGAAAGACA GGGAGCAAAT 
GTGGAGAGAC TGGCTAGTGT ATTATCAGAG GTCAGAAATC TGGAGGACCA GCTAGAGAGT 
GCTCAGATAG AGCTGAAAAA GGGGAAGACA GATGAAATGT CCTTAAAGAG ATTGGTCACT 
GCAAAACATG AGAAGCTGTC TACAGCTGAA ATAAGGATAA AAAAGAAGCG TGAAGATGTT 
GAGCAATATA AGCACACTAT ATTTGAATAT TGTAACAGAG TTCAGGAGAA GAGGGGTGCT 
GTGTATGATA AGGTGACGGC CATTCACAAG GAAATCCAAC AGACAAGATT CAAAATTCAG 
CAGCTGAATG AGAATGCTGA AAAAGAAGAC ATGAAAGCCA AGGAAATATA CCTAAATCTG 
AAGGCTGGGT TGGAGAAGCA TCATGACTCT CTCATTAAGA CAGCAAAGAG TTACGCAGCC 
TCAAGAGAAG ATAAAATTGC TGAACTGAAG AAGGGGCTGC TTAGAGTTCA GTCTCCCGGA 
AATAGTTCTT AG