NAE1 cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	NAE1
Entrez Gene ID	108505871
Full Name	NEDD8 activating enzyme E1 subunit 1
Gene Type	protein-coding
Organism	Lepidothrix coronata(blue-crowned manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017832278.1	XP_017687767.1	NEDD8-activating enzyme E1 regulatory subunit

Pathways from BioSystems
Gene Ontology

The following NAE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLe70340	XM_017832278.1 Latest version!	Lepidothrix coronata NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAE1 ( XM_017832278.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017687767.1 Lepidothrix coronata NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.

Price & Availability↑

CloneID	OLe70340
Clone ID Related Accession (Same CDS sequence)	XM_017832278.1
Accession Version	XM_017832278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	NEDD8-activating enzyme E1 regulatory subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690380.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGC CGGGCCGGGC CGCGGCCAAG GAGCAGCGCT ACGACCGCCA GCTCAGACTG 
TGGGGTGACC ATGGCCAGGA AGCATTAGAA TCTGCCCATG TTTGTGTGAT AAATGCCACA 
GCAACAGGAA CTGAAATACT CAAAAACTTA GTGCTGCCAG GTATTGGTTC CTTTACAATT 
GTCGACGGGA ATCAAGTCTC TGGAGAAGAT GTTGGAAATA ATTTCTTTCT ACAAAAAAGC 
CATATTGGCC AGAGTCGTGC CCAGAGTGCC ATGGAGCTCC TGCAGGAGTT GAATAATGAT 
GTTTCTGGAC ATTTTGTTGA AGAGAGTCCA GAAAAGCTCC TGGACAACAA CCCTTCCTTT 
TTTAATCGGT TTAACTTGGT GATTGCAACA CAACTACCAG AAAGTACTTT GCTGCGCTTG 
GCTGAACTTC TCTGGAATTC TGACATTCCT CTGCTGGTCT GCAGGACTTA TGGACTGGTT 
GGTTATATGA GAGTCGTTAT TAAAGAACAT ACAGTTGTTG AATCTCACCC TGACAATACA 
TTGGAAGATC TGAGACTGGA CAAGCCATTT CCAGAACTGA CAGAACACAT TCAGTCCTAC 
GACTTGGATC ATATGGACAA GAAGGACCAC AGCCACACTC CATGGGTTGT GATTGTAGCC 
AAGTATCTGA CAAAATGGTT CAATGAGAAA AGTGAACAGC TGCCCAAAAG TTACAAGGAA 
AAAGAAGCCT TCAGACAACT GATTCGACAA GGTATCTTAA AGAATGAAAA TGGAACCCCA 
GAAGATGAGG AAAACTTTGA AGAAGCTATA AAAAATGTGA ACACAGCGTT GAATACTACA 
GAGGTTCCAA GAGGCATTCA GGAGCTTTTT GATGATGATT GCTGTGTGAA GCTCACAGAG 
CAGTCACCTT CCTTCTGGAT TTTGGTTCGA GCTTTAAAGG AATTTGTGGC AAATGAAGGG 
CAAGGAAGCT TGCCTGTGAG GGGCACTATT CCTGATATGA TTGCAGACTC CAGTAAATTT 
ATCAAACTGC AAAATGTATA CCGGGAGAAA GCCAAGAAGG ACATTGCTGC TGTGGGTGCA 
CATGCTGCTA AATTGCTGCA GTCCCTGGGC AAGGCACCAG AGTCTATTTC AGAGAGAGAA 
TTAAAATTGC TTTGCAGCAA CGCAGCCTTT CTGCGGGTGG TGCGGTGCCG ATCCCTGGCT 
GAGGAGCACG GCCCGAACAC CTTCCACAGG GATGAAATCA TTTCCCACAT GGATAACCCA 
GACAGTGAGA TAGTGCTGTA CTTGATGCTG AGGGCTGTAA ATAGATTTTA CAAGCAGCAT 
GGGAGATATC CAGGTGTCTA CAACTACCAG GTGGAAGATG ATATTGGAAA ACTAAACTCT 
TGCCTTACTG GTTTCCTGCA AGAGCACGGG CTGTCGGTGG TGGTGAAGGA TGACTATGTT 
CGTGAGTTCT GTCGCTATGG AGCTGCTGAG CCCCACGCTG TTGCTGCCTT CATGGGAGGG 
GCTGCTGCAC AGGAGGTTAT CAAAGTCATT ACAGGGCAGT TCGTAATTTT TAATAACACC 
TTTATTTACA ATGGAATGTC ACAGACTTCA GCAACTTTCC AGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017687767.1
CDS	17..1624
Translation	MARPGRAAAKEQRYDRQLRLWGDHGQEALESAHVCVINATATGTEILKNLVLPGIGSFTIVDGNQVSGEDVGNNFFLQKSHIGQSRAQSAMELLQELNNDVSGHFVEESPEKLLDNNPSFFNRFNLVIATQLPESTLLRLAELLWNSDIPLLVCRTYGLVGYMRVVIKEHTVVESHPDNTLEDLRLDKPFPELTEHIQSYDLDHMDKKDHSHTPWVVIVAKYLTKWFNEKSEQLPKSYKEKEAFRQLIRQGILKNENGTPEDEENFEEAIKNVNTALNTTEVPRGIQELFDDDCCVKLTEQSPSFWILVRALKEFVANEGQGSLPVRGTIPDMIADSSKFIKLQNVYREKAKKDIAAVGAHAAKLLQSLGKAPESISERELKLLCSNAAFLRVVRCRSLAEEHGPNTFHRDEIISHMDNPDSEIVLYLMLRAVNRFYKQHGRYPGVYNYQVEDDIGKLNSCLTGFLQEHGLSVVVKDDYVREFCRYGAAEPHAVAAFMGGAAAQEVIKVITGQFVIFNNTFIYNGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_017832278.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017832278.1

ORF Insert Sequence:

ATGGCGCGGC CGGGCCGGGC CGCGGCCAAG GAGCAGCGCT ACGACCGCCA GCTCAGACTG 
TGGGGTGACC ATGGCCAGGA AGCATTAGAA TCTGCCCATG TTTGTGTGAT AAATGCCACA 
GCAACAGGAA CTGAAATACT CAAAAACTTA GTGCTGCCAG GTATTGGTTC CTTTACAATT 
GTCGACGGGA ATCAAGTCTC TGGAGAAGAT GTTGGAAATA ATTTCTTTCT ACAAAAAAGC 
CATATTGGCC AGAGTCGTGC CCAGAGTGCC ATGGAGCTCC TGCAGGAGTT GAATAATGAT 
GTTTCTGGAC ATTTTGTTGA AGAGAGTCCA GAAAAGCTCC TGGACAACAA CCCTTCCTTT 
TTTAATCGGT TTAACTTGGT GATTGCAACA CAACTACCAG AAAGTACTTT GCTGCGCTTG 
GCTGAACTTC TCTGGAATTC TGACATTCCT CTGCTGGTCT GCAGGACTTA TGGACTGGTT 
GGTTATATGA GAGTCGTTAT TAAAGAACAT ACAGTTGTTG AATCTCACCC TGACAATACA 
TTGGAAGATC TGAGACTGGA CAAGCCATTT CCAGAACTGA CAGAACACAT TCAGTCCTAC 
GACTTGGATC ATATGGACAA GAAGGACCAC AGCCACACTC CATGGGTTGT GATTGTAGCC 
AAGTATCTGA CAAAATGGTT CAATGAGAAA AGTGAACAGC TGCCCAAAAG TTACAAGGAA 
AAAGAAGCCT TCAGACAACT GATTCGACAA GGTATCTTAA AGAATGAAAA TGGAACCCCA 
GAAGATGAGG AAAACTTTGA AGAAGCTATA AAAAATGTGA ACACAGCGTT GAATACTACA 
GAGGTTCCAA GAGGCATTCA GGAGCTTTTT GATGATGATT GCTGTGTGAA GCTCACAGAG 
CAGTCACCTT CCTTCTGGAT TTTGGTTCGA GCTTTAAAGG AATTTGTGGC AAATGAAGGG 
CAAGGAAGCT TGCCTGTGAG GGGCACTATT CCTGATATGA TTGCAGACTC CAGTAAATTT 
ATCAAACTGC AAAATGTATA CCGGGAGAAA GCCAAGAAGG ACATTGCTGC TGTGGGTGCA 
CATGCTGCTA AATTGCTGCA GTCCCTGGGC AAGGCACCAG AGTCTATTTC AGAGAGAGAA 
TTAAAATTGC TTTGCAGCAA CGCAGCCTTT CTGCGGGTGG TGCGGTGCCG ATCCCTGGCT 
GAGGAGCACG GCCCGAACAC CTTCCACAGG GATGAAATCA TTTCCCACAT GGATAACCCA 
GACAGTGAGA TAGTGCTGTA CTTGATGCTG AGGGCTGTAA ATAGATTTTA CAAGCAGCAT 
GGGAGATATC CAGGTGTCTA CAACTACCAG GTGGAAGATG ATATTGGAAA ACTAAACTCT 
TGCCTTACTG GTTTCCTGCA AGAGCACGGG CTGTCGGTGG TGGTGAAGGA TGACTATGTT 
CGTGAGTTCT GTCGCTATGG AGCTGCTGAG CCCCACGCTG TTGCTGCCTT CATGGGAGGG 
GCTGCTGCAC AGGAGGTTAT CAAAGTCATT ACAGGGCAGT TCGTAATTTT TAATAACACC 
TTTATTTACA ATGGAATGTC ACAGACTTCA GCAACTTTCC AGCTGTAG