ANKLE2 cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKLE2
Entrez Gene ID	108497667
Full Name	ankyrin repeat and LEM domain containing 2
Gene Type	protein-coding
Organism	Lepidothrix coronata(blue-crowned manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017815501.1	XP_017670990.1	ankyrin repeat and LEM domain-containing protein 2
XM_017815500.1	XP_017670989.1	ankyrin repeat and LEM domain-containing protein 2

Pathways from BioSystems
Gene Ontology

The following ANKLE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKLE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLe58709	XM_017815501.1 Latest version!	Lepidothrix coronata ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OLe58709	XM_017815500.1 Latest version!	Lepidothrix coronata ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKLE2 ( XM_017815501.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017670990.1 Lepidothrix coronata ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLe58709
Clone ID Related Accession (Same CDS sequence)	XM_017815501.1 , XM_017815500.1
Accession Version	XM_017815501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	ankyrin repeat and LEM domain-containing protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690231.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACAA TACTATCCCG ACTGAAGAAG CTTGGCCCGG ATGAGCTTAG GGAAGAGATT 
GTGAGAGCAG GACTGAAATG CGGCCCCATT ACTGCAACTA CCAGGTTTAT TTTTGAAAAG 
AAATTGGCTC AGGCGTTGTT GGAGCAGCAA GGAGCACTGG AGGAACCAGG ATCTGCTCTG 
CCAGAGGAGG CTGCTGGAAA TTTGGCAGCA AATCACAACG GCCATGGAAG TGCTGCTGAG 
GAGCCAGACT TTGGGTACTG TGTGGGTCTG AACCCTCCAG AAGAAGATGC AGTGACACAC 
ATGAACTGTT CAGCTGCAGC CTGTGGAGCA GGAAGTGTTG ATTCACAAAC CAGTGCTCAG 
ACACCATCCA GAGATCCTCC ACTGTTCTAC GGTGTCTGTC CAGTTTATGA TGACATACTG 
GCCAGGAATG AGAGAGTACA TGTTTATGAA GATAAAAAGG AAGCTCTCCA AGCTGTTAAG 
ATGATTAAGG GTTCCCGTTT TAAGGCTTTT ACAAACAGAG AAGATGCTGA GAAATTTGCT 
AAGGGAATCT GTGATTATTT CCCATCTCCA AGCAAGTCCT CCTTATGTTT GTCACCAGTG 
AAAATGGGAT CATTTAACAG AGATGGCTTG TGCTCCCCTG AAACCGAAAC TGCTAATAAG 
GAGAGAGCCA ACAGCTATAA AAGTCCACGG ACTCAAGATC TCACGGCTAA ACTTCGGAAA 
GCTGTCGAGA AGGGAGACAC AGCAACGTTT TCAGAACTTA TTTGGAGTAA CCCTCGCTAC 
CTGATCGGGT CAGGAGACAA TCCAACGGTT GTACAGGAAG GGTGTAGGTA CAATGTCATG 
CATGTTGCTG CCAAGGAGAA TCAACCTGGG ATCTGCCAGC TGCTGCTGGA CACGTTGGAA 
AATCCTGAGT TTATGAGGCT CATGTACCCC GATGATAACG ATGCCATGCT GAAGAATCGC 
ATCCAATATA TTGTTGACCT TTACCTTAAC ACACCAGATA AAATGGGATT TGATACTCCA 
CTGCATTTTG CCTGCAAGTT TGGGAATTTG GATGTTGTTA ACGTGCTTAC CTCACACCCA 
GCTATTGTGA AAAATCCAAG AAACAAATAT GATCAAACTC CAGCAGAAGT AATTTGTGAA 
AGAAGCAAGA ATAAATCTGC AGAATTGAAA GAAAAGCTAA GAGAGTACTT GAAAGGCCGA 
TACTATGTGC CACTCTTGAG AGCAGAAGAC AACTCCTCAG CTCCTGTCAT TGGAGCGCCC 
TGGTCACCGG ATCAGATGGA TGATGGTCCC CAGAGGACTT TATTTAAATA CCCTGGCAAC 
CCCAAAGATC CAGTGTTGTC CATAAGAGCT TTTGCAGGCC CCATGAGCCC CTCAAAGGCT 
GAAGAATTTC GCAGGCTTTG GAAAACTCCA CCTCGAGAGA GAGCTGGCTT CTTTCACAAT 
GTCAGGAAAT CTGATCTGGA GAGAGGTGTT GAAAGAGTTG GAAGGGAGTT AGCTCATGAA 
CTGGGGTTCC CGTGGGTTGA ATACTGGGAA TTTCTGGGCT GTTTTGTTGA TCTGTCTTCC 
CAGGAGGGGC TGCGAAAATT AGAAGAGTAC CTGAGTCATC ATGAAATGAG CGCAAAGGCT 
CAGCAGGAGA CAGGGGAAAA TGAAACCTGC AACAGATATA AAACACCCCA GCCCTCGGGG 
AAGAGCAAAA AGTCCTGCAA TTCCATTTCC GTGGGTGCAT TTTTGGACGA GGATGAGGAT 
GACATGAGCT TAGAGGAAAT CAAAAACAGG CAGAACGCAG CACGGAACGT CAGCCCCGGG 
GCGGCGTCCC GGGAGCCCGT GGGCGACATC GCCGAGCGCG ACATCCTGGC CGTGGGGCGC 
GCGGCAAACA TCCTGCCCAC GGCCCCGCAC CACGGCAAGG AGGCTGCCCC CAGCAAAAAC 
GGGTTCTGTA GCCCCCTGTC CAGCGAGAGG ATCATCAGTG ACAGGAAGCA GCTGCACGAT 
GGGGAGGAAC GTCTCGTGTC TCCCGTCTCC AATTTGATGT CGGAGTTCGA AAGCCTGTCC 
TTCCAGGAAC AGGAGCTTGC AGGAGGGTCT CATCAAATCA CTGAAAAAAC TGAGAACGAG 
GGGATTCTGG ACAAGGCCTG CTGTGCAGTG TCACTGGCAA GTTCTTCTGA GCCGAGTGTT 
ACAGGAAAAT CTGGAGAGGC CAAGTTAAGG ACAGAACACA AAATGCCATT AGAAAAGGAG 
AGGCTGTCTA GGCAATCTGG GATTCTGACT AAGGAGGCAC AACAACGTCA AGAACTTTCT 
TCCCAGAAGT TTCTCTTGAA GCCTTTACCC ACAAATGACA ATTCTAAGTT ATTCCTGCTT 
GGGGAGCAGC CCTCGAAGCT GGATGGGGAT GTCCTGGCAG CTCTGGAAGG AGCAGAGATC 
GATGCTCAGA AATACCCGAT GATTTCCAGG TGGAAGCTCG CAGTGCAGTC CTACTCCTCT 
TCTGACAGGC AAAGTTGGCC CAGTCCAGCT CTGAAGGCAA AATTCCGGTC CCAAACCACC 
GCTGCTGGCC TTCCAAATGC TTCAGTGTAT CCCAGTTCAG GAAGAAACAG TCCCATACCC 
GGAAGTCCAG GAAAACACGG CAGTGCCACC TCGTTCCCTG CAGAGCCGGG CAGCCCCGGG 
CGCTACAGCC CCGCCTCTGT GCACCAGGCC CTGCTCAAAC TGCGCTACCC GGCAGAGCCC 
CCTGCCCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017670990.1
CDS	26..2737
Translation	MDTILSRLKKLGPDELREEIVRAGLKCGPITATTRFIFEKKLAQALLEQQGALEEPGSALPEEAAGNLAANHNGHGSAAEEPDFGYCVGLNPPEEDAVTHMNCSAAACGAGSVDSQTSAQTPSRDPPLFYGVCPVYDDILARNERVHVYEDKKEALQAVKMIKGSRFKAFTNREDAEKFAKGICDYFPSPSKSSLCLSPVKMGSFNRDGLCSPETETANKERANSYKSPRTQDLTAKLRKAVEKGDTATFSELIWSNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQPGICQLLLDTLENPEFMRLMYPDDNDAMLKNRIQYIVDLYLNTPDKMGFDTPLHFACKFGNLDVVNVLTSHPAIVKNPRNKYDQTPAEVICERSKNKSAELKEKLREYLKGRYYVPLLRAEDNSSAPVIGAPWSPDQMDDGPQRTLFKYPGNPKDPVLSIRAFAGPMSPSKAEEFRRLWKTPPRERAGFFHNVRKSDLERGVERVGRELAHELGFPWVEYWEFLGCFVDLSSQEGLRKLEEYLSHHEMSAKAQQETGENETCNRYKTPQPSGKSKKSCNSISVGAFLDEDEDDMSLEEIKNRQNAARNVSPGAASREPVGDIAERDILAVGRAANILPTAPHHGKEAAPSKNGFCSPLSSERIISDRKQLHDGEERLVSPVSNLMSEFESLSFQEQELAGGSHQITEKTENEGILDKACCAVSLASSSEPSVTGKSGEAKLRTEHKMPLEKERLSRQSGILTKEAQQRQELSSQKFLLKPLPTNDNSKLFLLGEQPSKLDGDVLAALEGAEIDAQKYPMISRWKLAVQSYSSSDRQSWPSPALKAKFRSQTTAAGLPNASVYPSSGRNSPIPGSPGKHGSATSFPAEPGSPGRYSPASVHQALLKLRYPAEPPAH

Target ORF information:

RefSeq Version	XM_017815501.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017815501.1

ORF Insert Sequence:

ATGGATACAA TACTATCCCG ACTGAAGAAG CTTGGCCCGG ATGAGCTTAG GGAAGAGATT 
GTGAGAGCAG GACTGAAATG CGGCCCCATT ACTGCAACTA CCAGGTTTAT TTTTGAAAAG 
AAATTGGCTC AGGCGTTGTT GGAGCAGCAA GGAGCACTGG AGGAACCAGG ATCTGCTCTG 
CCAGAGGAGG CTGCTGGAAA TTTGGCAGCA AATCACAACG GCCATGGAAG TGCTGCTGAG 
GAGCCAGACT TTGGGTACTG TGTGGGTCTG AACCCTCCAG AAGAAGATGC AGTGACACAC 
ATGAACTGTT CAGCTGCAGC CTGTGGAGCA GGAAGTGTTG ATTCACAAAC CAGTGCTCAG 
ACACCATCCA GAGATCCTCC ACTGTTCTAC GGTGTCTGTC CAGTTTATGA TGACATACTG 
GCCAGGAATG AGAGAGTACA TGTTTATGAA GATAAAAAGG AAGCTCTCCA AGCTGTTAAG 
ATGATTAAGG GTTCCCGTTT TAAGGCTTTT ACAAACAGAG AAGATGCTGA GAAATTTGCT 
AAGGGAATCT GTGATTATTT CCCATCTCCA AGCAAGTCCT CCTTATGTTT GTCACCAGTG 
AAAATGGGAT CATTTAACAG AGATGGCTTG TGCTCCCCTG AAACCGAAAC TGCTAATAAG 
GAGAGAGCCA ACAGCTATAA AAGTCCACGG ACTCAAGATC TCACGGCTAA ACTTCGGAAA 
GCTGTCGAGA AGGGAGACAC AGCAACGTTT TCAGAACTTA TTTGGAGTAA CCCTCGCTAC 
CTGATCGGGT CAGGAGACAA TCCAACGGTT GTACAGGAAG GGTGTAGGTA CAATGTCATG 
CATGTTGCTG CCAAGGAGAA TCAACCTGGG ATCTGCCAGC TGCTGCTGGA CACGTTGGAA 
AATCCTGAGT TTATGAGGCT CATGTACCCC GATGATAACG ATGCCATGCT GAAGAATCGC 
ATCCAATATA TTGTTGACCT TTACCTTAAC ACACCAGATA AAATGGGATT TGATACTCCA 
CTGCATTTTG CCTGCAAGTT TGGGAATTTG GATGTTGTTA ACGTGCTTAC CTCACACCCA 
GCTATTGTGA AAAATCCAAG AAACAAATAT GATCAAACTC CAGCAGAAGT AATTTGTGAA 
AGAAGCAAGA ATAAATCTGC AGAATTGAAA GAAAAGCTAA GAGAGTACTT GAAAGGCCGA 
TACTATGTGC CACTCTTGAG AGCAGAAGAC AACTCCTCAG CTCCTGTCAT TGGAGCGCCC 
TGGTCACCGG ATCAGATGGA TGATGGTCCC CAGAGGACTT TATTTAAATA CCCTGGCAAC 
CCCAAAGATC CAGTGTTGTC CATAAGAGCT TTTGCAGGCC CCATGAGCCC CTCAAAGGCT 
GAAGAATTTC GCAGGCTTTG GAAAACTCCA CCTCGAGAGA GAGCTGGCTT CTTTCACAAT 
GTCAGGAAAT CTGATCTGGA GAGAGGTGTT GAAAGAGTTG GAAGGGAGTT AGCTCATGAA 
CTGGGGTTCC CGTGGGTTGA ATACTGGGAA TTTCTGGGCT GTTTTGTTGA TCTGTCTTCC 
CAGGAGGGGC TGCGAAAATT AGAAGAGTAC CTGAGTCATC ATGAAATGAG CGCAAAGGCT 
CAGCAGGAGA CAGGGGAAAA TGAAACCTGC AACAGATATA AAACACCCCA GCCCTCGGGG 
AAGAGCAAAA AGTCCTGCAA TTCCATTTCC GTGGGTGCAT TTTTGGACGA GGATGAGGAT 
GACATGAGCT TAGAGGAAAT CAAAAACAGG CAGAACGCAG CACGGAACGT CAGCCCCGGG 
GCGGCGTCCC GGGAGCCCGT GGGCGACATC GCCGAGCGCG ACATCCTGGC CGTGGGGCGC 
GCGGCAAACA TCCTGCCCAC GGCCCCGCAC CACGGCAAGG AGGCTGCCCC CAGCAAAAAC 
GGGTTCTGTA GCCCCCTGTC CAGCGAGAGG ATCATCAGTG ACAGGAAGCA GCTGCACGAT 
GGGGAGGAAC GTCTCGTGTC TCCCGTCTCC AATTTGATGT CGGAGTTCGA AAGCCTGTCC 
TTCCAGGAAC AGGAGCTTGC AGGAGGGTCT CATCAAATCA CTGAAAAAAC TGAGAACGAG 
GGGATTCTGG ACAAGGCCTG CTGTGCAGTG TCACTGGCAA GTTCTTCTGA GCCGAGTGTT 
ACAGGAAAAT CTGGAGAGGC CAAGTTAAGG ACAGAACACA AAATGCCATT AGAAAAGGAG 
AGGCTGTCTA GGCAATCTGG GATTCTGACT AAGGAGGCAC AACAACGTCA AGAACTTTCT 
TCCCAGAAGT TTCTCTTGAA GCCTTTACCC ACAAATGACA ATTCTAAGTT ATTCCTGCTT 
GGGGAGCAGC CCTCGAAGCT GGATGGGGAT GTCCTGGCAG CTCTGGAAGG AGCAGAGATC 
GATGCTCAGA AATACCCGAT GATTTCCAGG TGGAAGCTCG CAGTGCAGTC CTACTCCTCT 
TCTGACAGGC AAAGTTGGCC CAGTCCAGCT CTGAAGGCAA AATTCCGGTC CCAAACCACC 
GCTGCTGGCC TTCCAAATGC TTCAGTGTAT CCCAGTTCAG GAAGAAACAG TCCCATACCC 
GGAAGTCCAG GAAAACACGG CAGTGCCACC TCGTTCCCTG CAGAGCCGGG CAGCCCCGGG 
CGCTACAGCC CCGCCTCTGT GCACCAGGCC CTGCTCAAAC TGCGCTACCC GGCAGAGCCC 
CCTGCCCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE2 ( XM_017815500.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017670989.1 Lepidothrix coronata ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLe58709
Clone ID Related Accession (Same CDS sequence)	XM_017815501.1 , XM_017815500.1
Accession Version	XM_017815500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	ankyrin repeat and LEM domain-containing protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690231.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACAA TACTATCCCG ACTGAAGAAG CTTGGCCCGG ATGAGCTTAG GGAAGAGATT 
GTGAGAGCAG GACTGAAATG CGGCCCCATT ACTGCAACTA CCAGGTTTAT TTTTGAAAAG 
AAATTGGCTC AGGCGTTGTT GGAGCAGCAA GGAGCACTGG AGGAACCAGG ATCTGCTCTG 
CCAGAGGAGG CTGCTGGAAA TTTGGCAGCA AATCACAACG GCCATGGAAG TGCTGCTGAG 
GAGCCAGACT TTGGGTACTG TGTGGGTCTG AACCCTCCAG AAGAAGATGC AGTGACACAC 
ATGAACTGTT CAGCTGCAGC CTGTGGAGCA GGAAGTGTTG ATTCACAAAC CAGTGCTCAG 
ACACCATCCA GAGATCCTCC ACTGTTCTAC GGTGTCTGTC CAGTTTATGA TGACATACTG 
GCCAGGAATG AGAGAGTACA TGTTTATGAA GATAAAAAGG AAGCTCTCCA AGCTGTTAAG 
ATGATTAAGG GTTCCCGTTT TAAGGCTTTT ACAAACAGAG AAGATGCTGA GAAATTTGCT 
AAGGGAATCT GTGATTATTT CCCATCTCCA AGCAAGTCCT CCTTATGTTT GTCACCAGTG 
AAAATGGGAT CATTTAACAG AGATGGCTTG TGCTCCCCTG AAACCGAAAC TGCTAATAAG 
GAGAGAGCCA ACAGCTATAA AAGTCCACGG ACTCAAGATC TCACGGCTAA ACTTCGGAAA 
GCTGTCGAGA AGGGAGACAC AGCAACGTTT TCAGAACTTA TTTGGAGTAA CCCTCGCTAC 
CTGATCGGGT CAGGAGACAA TCCAACGGTT GTACAGGAAG GGTGTAGGTA CAATGTCATG 
CATGTTGCTG CCAAGGAGAA TCAACCTGGG ATCTGCCAGC TGCTGCTGGA CACGTTGGAA 
AATCCTGAGT TTATGAGGCT CATGTACCCC GATGATAACG ATGCCATGCT GAAGAATCGC 
ATCCAATATA TTGTTGACCT TTACCTTAAC ACACCAGATA AAATGGGATT TGATACTCCA 
CTGCATTTTG CCTGCAAGTT TGGGAATTTG GATGTTGTTA ACGTGCTTAC CTCACACCCA 
GCTATTGTGA AAAATCCAAG AAACAAATAT GATCAAACTC CAGCAGAAGT AATTTGTGAA 
AGAAGCAAGA ATAAATCTGC AGAATTGAAA GAAAAGCTAA GAGAGTACTT GAAAGGCCGA 
TACTATGTGC CACTCTTGAG AGCAGAAGAC AACTCCTCAG CTCCTGTCAT TGGAGCGCCC 
TGGTCACCGG ATCAGATGGA TGATGGTCCC CAGAGGACTT TATTTAAATA CCCTGGCAAC 
CCCAAAGATC CAGTGTTGTC CATAAGAGCT TTTGCAGGCC CCATGAGCCC CTCAAAGGCT 
GAAGAATTTC GCAGGCTTTG GAAAACTCCA CCTCGAGAGA GAGCTGGCTT CTTTCACAAT 
GTCAGGAAAT CTGATCTGGA GAGAGGTGTT GAAAGAGTTG GAAGGGAGTT AGCTCATGAA 
CTGGGGTTCC CGTGGGTTGA ATACTGGGAA TTTCTGGGCT GTTTTGTTGA TCTGTCTTCC 
CAGGAGGGGC TGCGAAAATT AGAAGAGTAC CTGAGTCATC ATGAAATGAG CGCAAAGGCT 
CAGCAGGAGA CAGGGGAAAA TGAAACCTGC AACAGATATA AAACACCCCA GCCCTCGGGG 
AAGAGCAAAA AGTCCTGCAA TTCCATTTCC GTGGGTGCAT TTTTGGACGA GGATGAGGAT 
GACATGAGCT TAGAGGAAAT CAAAAACAGG CAGAACGCAG CACGGAACGT CAGCCCCGGG 
GCGGCGTCCC GGGAGCCCGT GGGCGACATC GCCGAGCGCG ACATCCTGGC CGTGGGGCGC 
GCGGCAAACA TCCTGCCCAC GGCCCCGCAC CACGGCAAGG AGGCTGCCCC CAGCAAAAAC 
GGGTTCTGTA GCCCCCTGTC CAGCGAGAGG ATCATCAGTG ACAGGAAGCA GCTGCACGAT 
GGGGAGGAAC GTCTCGTGTC TCCCGTCTCC AATTTGATGT CGGAGTTCGA AAGCCTGTCC 
TTCCAGGAAC AGGAGCTTGC AGGAGGGTCT CATCAAATCA CTGAAAAAAC TGAGAACGAG 
GGGATTCTGG ACAAGGCCTG CTGTGCAGTG TCACTGGCAA GTTCTTCTGA GCCGAGTGTT 
ACAGGAAAAT CTGGAGAGGC CAAGTTAAGG ACAGAACACA AAATGCCATT AGAAAAGGAG 
AGGCTGTCTA GGCAATCTGG GATTCTGACT AAGGAGGCAC AACAACGTCA AGAACTTTCT 
TCCCAGAAGT TTCTCTTGAA GCCTTTACCC ACAAATGACA ATTCTAAGTT ATTCCTGCTT 
GGGGAGCAGC CCTCGAAGCT GGATGGGGAT GTCCTGGCAG CTCTGGAAGG AGCAGAGATC 
GATGCTCAGA AATACCCGAT GATTTCCAGG TGGAAGCTCG CAGTGCAGTC CTACTCCTCT 
TCTGACAGGC AAAGTTGGCC CAGTCCAGCT CTGAAGGCAA AATTCCGGTC CCAAACCACC 
GCTGCTGGCC TTCCAAATGC TTCAGTGTAT CCCAGTTCAG GAAGAAACAG TCCCATACCC 
GGAAGTCCAG GAAAACACGG CAGTGCCACC TCGTTCCCTG CAGAGCCGGG CAGCCCCGGG 
CGCTACAGCC CCGCCTCTGT GCACCAGGCC CTGCTCAAAC TGCGCTACCC GGCAGAGCCC 
CCTGCCCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017670989.1
CDS	80..2791
Translation	MDTILSRLKKLGPDELREEIVRAGLKCGPITATTRFIFEKKLAQALLEQQGALEEPGSALPEEAAGNLAANHNGHGSAAEEPDFGYCVGLNPPEEDAVTHMNCSAAACGAGSVDSQTSAQTPSRDPPLFYGVCPVYDDILARNERVHVYEDKKEALQAVKMIKGSRFKAFTNREDAEKFAKGICDYFPSPSKSSLCLSPVKMGSFNRDGLCSPETETANKERANSYKSPRTQDLTAKLRKAVEKGDTATFSELIWSNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQPGICQLLLDTLENPEFMRLMYPDDNDAMLKNRIQYIVDLYLNTPDKMGFDTPLHFACKFGNLDVVNVLTSHPAIVKNPRNKYDQTPAEVICERSKNKSAELKEKLREYLKGRYYVPLLRAEDNSSAPVIGAPWSPDQMDDGPQRTLFKYPGNPKDPVLSIRAFAGPMSPSKAEEFRRLWKTPPRERAGFFHNVRKSDLERGVERVGRELAHELGFPWVEYWEFLGCFVDLSSQEGLRKLEEYLSHHEMSAKAQQETGENETCNRYKTPQPSGKSKKSCNSISVGAFLDEDEDDMSLEEIKNRQNAARNVSPGAASREPVGDIAERDILAVGRAANILPTAPHHGKEAAPSKNGFCSPLSSERIISDRKQLHDGEERLVSPVSNLMSEFESLSFQEQELAGGSHQITEKTENEGILDKACCAVSLASSSEPSVTGKSGEAKLRTEHKMPLEKERLSRQSGILTKEAQQRQELSSQKFLLKPLPTNDNSKLFLLGEQPSKLDGDVLAALEGAEIDAQKYPMISRWKLAVQSYSSSDRQSWPSPALKAKFRSQTTAAGLPNASVYPSSGRNSPIPGSPGKHGSATSFPAEPGSPGRYSPASVHQALLKLRYPAEPPAH

Target ORF information:

RefSeq Version	XM_017815500.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017815500.1

ORF Insert Sequence:

ATGGATACAA TACTATCCCG ACTGAAGAAG CTTGGCCCGG ATGAGCTTAG GGAAGAGATT 
GTGAGAGCAG GACTGAAATG CGGCCCCATT ACTGCAACTA CCAGGTTTAT TTTTGAAAAG 
AAATTGGCTC AGGCGTTGTT GGAGCAGCAA GGAGCACTGG AGGAACCAGG ATCTGCTCTG 
CCAGAGGAGG CTGCTGGAAA TTTGGCAGCA AATCACAACG GCCATGGAAG TGCTGCTGAG 
GAGCCAGACT TTGGGTACTG TGTGGGTCTG AACCCTCCAG AAGAAGATGC AGTGACACAC 
ATGAACTGTT CAGCTGCAGC CTGTGGAGCA GGAAGTGTTG ATTCACAAAC CAGTGCTCAG 
ACACCATCCA GAGATCCTCC ACTGTTCTAC GGTGTCTGTC CAGTTTATGA TGACATACTG 
GCCAGGAATG AGAGAGTACA TGTTTATGAA GATAAAAAGG AAGCTCTCCA AGCTGTTAAG 
ATGATTAAGG GTTCCCGTTT TAAGGCTTTT ACAAACAGAG AAGATGCTGA GAAATTTGCT 
AAGGGAATCT GTGATTATTT CCCATCTCCA AGCAAGTCCT CCTTATGTTT GTCACCAGTG 
AAAATGGGAT CATTTAACAG AGATGGCTTG TGCTCCCCTG AAACCGAAAC TGCTAATAAG 
GAGAGAGCCA ACAGCTATAA AAGTCCACGG ACTCAAGATC TCACGGCTAA ACTTCGGAAA 
GCTGTCGAGA AGGGAGACAC AGCAACGTTT TCAGAACTTA TTTGGAGTAA CCCTCGCTAC 
CTGATCGGGT CAGGAGACAA TCCAACGGTT GTACAGGAAG GGTGTAGGTA CAATGTCATG 
CATGTTGCTG CCAAGGAGAA TCAACCTGGG ATCTGCCAGC TGCTGCTGGA CACGTTGGAA 
AATCCTGAGT TTATGAGGCT CATGTACCCC GATGATAACG ATGCCATGCT GAAGAATCGC 
ATCCAATATA TTGTTGACCT TTACCTTAAC ACACCAGATA AAATGGGATT TGATACTCCA 
CTGCATTTTG CCTGCAAGTT TGGGAATTTG GATGTTGTTA ACGTGCTTAC CTCACACCCA 
GCTATTGTGA AAAATCCAAG AAACAAATAT GATCAAACTC CAGCAGAAGT AATTTGTGAA 
AGAAGCAAGA ATAAATCTGC AGAATTGAAA GAAAAGCTAA GAGAGTACTT GAAAGGCCGA 
TACTATGTGC CACTCTTGAG AGCAGAAGAC AACTCCTCAG CTCCTGTCAT TGGAGCGCCC 
TGGTCACCGG ATCAGATGGA TGATGGTCCC CAGAGGACTT TATTTAAATA CCCTGGCAAC 
CCCAAAGATC CAGTGTTGTC CATAAGAGCT TTTGCAGGCC CCATGAGCCC CTCAAAGGCT 
GAAGAATTTC GCAGGCTTTG GAAAACTCCA CCTCGAGAGA GAGCTGGCTT CTTTCACAAT 
GTCAGGAAAT CTGATCTGGA GAGAGGTGTT GAAAGAGTTG GAAGGGAGTT AGCTCATGAA 
CTGGGGTTCC CGTGGGTTGA ATACTGGGAA TTTCTGGGCT GTTTTGTTGA TCTGTCTTCC 
CAGGAGGGGC TGCGAAAATT AGAAGAGTAC CTGAGTCATC ATGAAATGAG CGCAAAGGCT 
CAGCAGGAGA CAGGGGAAAA TGAAACCTGC AACAGATATA AAACACCCCA GCCCTCGGGG 
AAGAGCAAAA AGTCCTGCAA TTCCATTTCC GTGGGTGCAT TTTTGGACGA GGATGAGGAT 
GACATGAGCT TAGAGGAAAT CAAAAACAGG CAGAACGCAG CACGGAACGT CAGCCCCGGG 
GCGGCGTCCC GGGAGCCCGT GGGCGACATC GCCGAGCGCG ACATCCTGGC CGTGGGGCGC 
GCGGCAAACA TCCTGCCCAC GGCCCCGCAC CACGGCAAGG AGGCTGCCCC CAGCAAAAAC 
GGGTTCTGTA GCCCCCTGTC CAGCGAGAGG ATCATCAGTG ACAGGAAGCA GCTGCACGAT 
GGGGAGGAAC GTCTCGTGTC TCCCGTCTCC AATTTGATGT CGGAGTTCGA AAGCCTGTCC 
TTCCAGGAAC AGGAGCTTGC AGGAGGGTCT CATCAAATCA CTGAAAAAAC TGAGAACGAG 
GGGATTCTGG ACAAGGCCTG CTGTGCAGTG TCACTGGCAA GTTCTTCTGA GCCGAGTGTT 
ACAGGAAAAT CTGGAGAGGC CAAGTTAAGG ACAGAACACA AAATGCCATT AGAAAAGGAG 
AGGCTGTCTA GGCAATCTGG GATTCTGACT AAGGAGGCAC AACAACGTCA AGAACTTTCT 
TCCCAGAAGT TTCTCTTGAA GCCTTTACCC ACAAATGACA ATTCTAAGTT ATTCCTGCTT 
GGGGAGCAGC CCTCGAAGCT GGATGGGGAT GTCCTGGCAG CTCTGGAAGG AGCAGAGATC 
GATGCTCAGA AATACCCGAT GATTTCCAGG TGGAAGCTCG CAGTGCAGTC CTACTCCTCT 
TCTGACAGGC AAAGTTGGCC CAGTCCAGCT CTGAAGGCAA AATTCCGGTC CCAAACCACC 
GCTGCTGGCC TTCCAAATGC TTCAGTGTAT CCCAGTTCAG GAAGAAACAG TCCCATACCC 
GGAAGTCCAG GAAAACACGG CAGTGCCACC TCGTTCCCTG CAGAGCCGGG CAGCCCCGGG 
CGCTACAGCC CCGCCTCTGT GCACCAGGCC CTGCTCAAAC TGCGCTACCC GGCAGAGCCC 
CCTGCCCACT AA