EIF2B5 cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF2B5
Entrez Gene ID	108495187
Full Name	eukaryotic translation initiation factor 2B subunit epsilon
Gene Type	protein-coding
Organism	Lepidothrix coronata(blue-crowned manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017810547.1	XP_017666036.1	translation initiation factor eIF-2B subunit epsilon

Pathways from BioSystems
Gene Ontology

The following EIF2B5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF2B5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLe55274	XM_017810547.1 Latest version!	Lepidothrix coronata eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF2B5 ( XM_017810547.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017666036.1 Lepidothrix coronata eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), mRNA.

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CloneID	OLe55274
Clone ID Related Accession (Same CDS sequence)	XM_017810547.1
Accession Version	XM_017810547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	translation initiation factor eIF-2B subunit epsilon
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690209.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC GCGGCACCGC GCGGCGCGGG CCGGGCGCGG GGGCCGCCCG CGGGCCCGAC 
CCGCAGGAGG AGCCGCCGCC GCCGCTCCAG GCCGTGCTAG TCGCCGACAG CTTCAACCGC 
CGCTTTTTCC CGATCTCCAA GGACCGGCCA CGGGCTCTTT TACCTATGGC AAATGTGGCT 
ATGATTGACT ATACCTTGGA ATTCCTAACA GCAACTGGAG TGGAAGAAAC TTTTGTTTTC 
TGCTGTTGGA AGTCAGCTGA GATAAAAGAG CATTTGCAAA AATCCAAGTG GTGCCGCCAC 
ACGTCTCCCA ACACGGTGCG GTTTGTGACC TCGGACCTGT ACCGCTCCCT CGGTGACGTC 
CTGCGGGACG TGGATGCCAA GTCCCTCGTT CGTTCCGACT TCATCCTCGT CACTGGGGAT 
GTGGTGTCCA ATTTCAATAT ATCCAAAGCC CTGGAGGAGC ACAAGTTGCG TCGTAAGATG 
GAGAAGAATG TTTCTGTGAT GACTATGATA TTCAAGGAGT CCTCCCCTGG CCACCATGCC 
AGGTGCAAAG AGGATGACAT TGTTATTGCT ATGGACAGTG CCACAAACCG TGTCCTTCAC 
TACCAGAGAA CCCAGGGGCT GAAACGATTC CGCTTTCCTA TGAGTCTGTT CCAGAGCTCT 
ATTGAGAATG TCGAGGTGCG CCATGACTTG CTGGATTGTC ACATCAGCAT CTGCTCTCCA 
CAGGTGGCTG AGCTGTTCAC AGACAATTTT GACTACCAGA CACGGGATGA CTTTGTGCGT 
GGTCTGTTGG TGAATGAGGA GGTCCTGGGG AACCAGATCC ATATGCATGT AACGACGGAA 
GAGTATGGTG CTCATATATG CAACCTGCTG ATGTATGAGG CTGTGTGCTC CGACATCATC 
CGGCGCTGGG TTTATCCTCT CACTCCAGAG ATGAACTTCA CTGATGACAA GAACCAGAGT 
TACACGCATT CCAAACACAA CATTTACCGA GGGGTGGATG TTAGCCTTGG CCACGGCAGC 
ATCCTGGAAG AGAATGTGCT CATCGGGCAG GGAACGGTCA TTGGCAGCAA CTGCTCCATC 
ACAAACAGTG TTATTGGGCA GAACTGTAGG ATAGGTGATA AAGTTGTTTT GGACGGAGCT 
TTCCTGTGGG ACAGAGTACA CATAGCTGAT AATGTGGAGA TCTACCATTC TGTTGTCTGT 
GACGAGGCTG AAGTGAAGGA GAAAGTAAAG CTAAAGCCCC GCTGTGTCCT CTCTTCTCAG 
GTAGTAGTAG GTCCTGGCAT TACTCTCTCT GAGGGCACAG TGATCTCTCT GCACCCCCCA 
GATGAGGAGG AAGAGGACGA TGACCAGTTC AGCGATGATT CTGGTGTGAA CAAGGAGGAG 
AGCAAAGTGA AACTGAAAGG TTACAATAAA AAGGACGTAG GCTCAGAGGG CAGAGGCTAT 
CTCTGGAAAC CAGATGACAA GAATGAAGAG GATGAAGAAG AGCAGAGACA AAGCCTATGG 
GGCCCAGCTA TGCATTCAGA GGAAGAGAGC GAGTCAGACA GTGACCTGAG CATGGGCTCT 
GAGGAGCCAG ATAGTCGGGC AGCATCCCCT CAGCTGGATG ACATCAAAGT TTTCCAGAAT 
GAGGTTCTGG GTACATTACA GAGGGGTGAA GAAGAAAACA TTTCCTGTGA CAACCTGGTC 
CTGGAAATAA ACTCCCTCAA ATACGCATAT AACATTGGCC TGAAGGAGAT GATGCAGGTG 
CTCAGTAAAG TGATCCTGGA GTTCCCACTG CAGCAGCTGG ATGCAAACCT GGACTCCCAA 
AGCTATTTCT CAGCGTTACT TCCTCTGTTG AAGAACTGGA CCCCGTTGTT TAAGAACTAC 
ATAAAACGAG CATCAGATCA CTTGAATGCC TTATGCGCCA TTGAGGAATT CTTCCTGGAA 
CATGACAGTC TCTGTGCATC GATAGCTAAA GTGTTGATGA CATTTTACCA GCTGGAAATT 
CTGGAAGAAG ATGTAATTCT CAATTGGTTC TCTTTGCGGG ACACGTCTGA CAAAGGAAAG 
CAGCTTCGGA AAAACCAACG GCTCCAGAAG TTTATCCAAT GGCTGGAGGA GGCAGAGGAG 
GAGTCGTCTG ATGGCGACCA AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017666036.1
CDS	1..2127
Translation	MAARGTARRGPGAGAARGPDPQEEPPPPLQAVLVADSFNRRFFPISKDRPRALLPMANVAMIDYTLEFLTATGVEETFVFCCWKSAEIKEHLQKSKWCRHTSPNTVRFVTSDLYRSLGDVLRDVDAKSLVRSDFILVTGDVVSNFNISKALEEHKLRRKMEKNVSVMTMIFKESSPGHHARCKEDDIVIAMDSATNRVLHYQRTQGLKRFRFPMSLFQSSIENVEVRHDLLDCHISICSPQVAELFTDNFDYQTRDDFVRGLLVNEEVLGNQIHMHVTTEEYGAHICNLLMYEAVCSDIIRRWVYPLTPEMNFTDDKNQSYTHSKHNIYRGVDVSLGHGSILEENVLIGQGTVIGSNCSITNSVIGQNCRIGDKVVLDGAFLWDRVHIADNVEIYHSVVCDEAEVKEKVKLKPRCVLSSQVVVGPGITLSEGTVISLHPPDEEEEDDDQFSDDSGVNKEESKVKLKGYNKKDVGSEGRGYLWKPDDKNEEDEEEQRQSLWGPAMHSEEESESDSDLSMGSEEPDSRAASPQLDDIKVFQNEVLGTLQRGEEENISCDNLVLEINSLKYAYNIGLKEMMQVLSKVILEFPLQQLDANLDSQSYFSALLPLLKNWTPLFKNYIKRASDHLNALCAIEEFFLEHDSLCASIAKVLMTFYQLEILEEDVILNWFSLRDTSDKGKQLRKNQRLQKFIQWLEEAEEESSDGDQD

Target ORF information:

RefSeq Version	XM_017810547.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017810547.1

ORF Insert Sequence:

ATGGCGGCCC GCGGCACCGC GCGGCGCGGG CCGGGCGCGG GGGCCGCCCG CGGGCCCGAC 
CCGCAGGAGG AGCCGCCGCC GCCGCTCCAG GCCGTGCTAG TCGCCGACAG CTTCAACCGC 
CGCTTTTTCC CGATCTCCAA GGACCGGCCA CGGGCTCTTT TACCTATGGC AAATGTGGCT 
ATGATTGACT ATACCTTGGA ATTCCTAACA GCAACTGGAG TGGAAGAAAC TTTTGTTTTC 
TGCTGTTGGA AGTCAGCTGA GATAAAAGAG CATTTGCAAA AATCCAAGTG GTGCCGCCAC 
ACGTCTCCCA ACACGGTGCG GTTTGTGACC TCGGACCTGT ACCGCTCCCT CGGTGACGTC 
CTGCGGGACG TGGATGCCAA GTCCCTCGTT CGTTCCGACT TCATCCTCGT CACTGGGGAT 
GTGGTGTCCA ATTTCAATAT ATCCAAAGCC CTGGAGGAGC ACAAGTTGCG TCGTAAGATG 
GAGAAGAATG TTTCTGTGAT GACTATGATA TTCAAGGAGT CCTCCCCTGG CCACCATGCC 
AGGTGCAAAG AGGATGACAT TGTTATTGCT ATGGACAGTG CCACAAACCG TGTCCTTCAC 
TACCAGAGAA CCCAGGGGCT GAAACGATTC CGCTTTCCTA TGAGTCTGTT CCAGAGCTCT 
ATTGAGAATG TCGAGGTGCG CCATGACTTG CTGGATTGTC ACATCAGCAT CTGCTCTCCA 
CAGGTGGCTG AGCTGTTCAC AGACAATTTT GACTACCAGA CACGGGATGA CTTTGTGCGT 
GGTCTGTTGG TGAATGAGGA GGTCCTGGGG AACCAGATCC ATATGCATGT AACGACGGAA 
GAGTATGGTG CTCATATATG CAACCTGCTG ATGTATGAGG CTGTGTGCTC CGACATCATC 
CGGCGCTGGG TTTATCCTCT CACTCCAGAG ATGAACTTCA CTGATGACAA GAACCAGAGT 
TACACGCATT CCAAACACAA CATTTACCGA GGGGTGGATG TTAGCCTTGG CCACGGCAGC 
ATCCTGGAAG AGAATGTGCT CATCGGGCAG GGAACGGTCA TTGGCAGCAA CTGCTCCATC 
ACAAACAGTG TTATTGGGCA GAACTGTAGG ATAGGTGATA AAGTTGTTTT GGACGGAGCT 
TTCCTGTGGG ACAGAGTACA CATAGCTGAT AATGTGGAGA TCTACCATTC TGTTGTCTGT 
GACGAGGCTG AAGTGAAGGA GAAAGTAAAG CTAAAGCCCC GCTGTGTCCT CTCTTCTCAG 
GTAGTAGTAG GTCCTGGCAT TACTCTCTCT GAGGGCACAG TGATCTCTCT GCACCCCCCA 
GATGAGGAGG AAGAGGACGA TGACCAGTTC AGCGATGATT CTGGTGTGAA CAAGGAGGAG 
AGCAAAGTGA AACTGAAAGG TTACAATAAA AAGGACGTAG GCTCAGAGGG CAGAGGCTAT 
CTCTGGAAAC CAGATGACAA GAATGAAGAG GATGAAGAAG AGCAGAGACA AAGCCTATGG 
GGCCCAGCTA TGCATTCAGA GGAAGAGAGC GAGTCAGACA GTGACCTGAG CATGGGCTCT 
GAGGAGCCAG ATAGTCGGGC AGCATCCCCT CAGCTGGATG ACATCAAAGT TTTCCAGAAT 
GAGGTTCTGG GTACATTACA GAGGGGTGAA GAAGAAAACA TTTCCTGTGA CAACCTGGTC 
CTGGAAATAA ACTCCCTCAA ATACGCATAT AACATTGGCC TGAAGGAGAT GATGCAGGTG 
CTCAGTAAAG TGATCCTGGA GTTCCCACTG CAGCAGCTGG ATGCAAACCT GGACTCCCAA 
AGCTATTTCT CAGCGTTACT TCCTCTGTTG AAGAACTGGA CCCCGTTGTT TAAGAACTAC 
ATAAAACGAG CATCAGATCA CTTGAATGCC TTATGCGCCA TTGAGGAATT CTTCCTGGAA 
CATGACAGTC TCTGTGCATC GATAGCTAAA GTGTTGATGA CATTTTACCA GCTGGAAATT 
CTGGAAGAAG ATGTAATTCT CAATTGGTTC TCTTTGCGGG ACACGTCTGA CAAAGGAAAG 
CAGCTTCGGA AAAACCAACG GCTCCAGAAG TTTATCCAAT GGCTGGAGGA GGCAGAGGAG 
GAGTCGTCTG ATGGCGACCA AGACTGA