crim1 cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus)

Gene
Clones
gRNAs

Summary

Gene Symbol	crim1
Entrez Gene ID	108233997
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Kryptolebias marmoratus(mangrove rivulus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017412805.1	XP_017268294.1	cysteine-rich motor neuron 1 protein
XM_017412805.2	XP_017268294.1	cysteine-rich motor neuron 1 protein

Pathways from BioSystems
Gene Ontology

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OKr00921	XM_017412805.2 Latest version!	Kryptolebias marmoratus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OKr00921	XM_017412805.1	Kryptolebias marmoratus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_017412805.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017268294.1 Kryptolebias marmoratus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Price & Availability↑

CloneID	OKr00921
Clone ID Related Accession (Same CDS sequence)	XM_017412805.1 , XM_017412805.2
Accession Version	XM_017412805.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094241.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGGCA AAGGCTCCGT TATCTCTCTG AGCGTCCTCA TTCTGGTGCG CGCGCTCCTG 
GCTCACGAGT GCACGCCTTG TAACCTCCGG ACGTGTCCCC TCAAAGCCCC GCGCGGTGCC 
TGCGAGGGCG GCCGGAGTGT GGCGAGGGAC CCCTGCGGAT GCTGCGAGCA GTGCGCGCGG 
CTGGAGTGGG AAACGTGCGG CGGTCCGGAC TGGACGCACG GATACTGCGC CCTGGGGCTG 
AGCTGCGCCT CCGTCAACGC GACAGGAGCT GCCACCGTCC CTGAAACTGG AGTGTGTAAA 
GTGCTTCCTG ACCATCCTAA TGCAGGTCTC GAGGATGAAC GCTGTCCACT GATGACAGGC 
TGCGACAGAT CGGGGGGTCA GTGTGTGTGC GATGCACGTC ACTCCTGCTT GGGATCTTTT 
ACCTATCCGA ACCGAGAAGC CTGTTTGAAG GCCAGCAAGT CAGATGGTCG AAAGCACGAG 
CACCGGGAGA GACACAGGGA GAAGTACGTC GGACCGGCCA ATCCAGAGTG CATGTTCTCC 
GGGTGTAACC TGACTGCTGA GGGCTGCATT TGCGAGTCTC AGAGCTGCCA CCACCACTTT 
GCCTACATCA ATCGCAGCCA GTGCCAGGAA GCAGCAGAAC AGCTGAGGTG TGCTGGGGTG 
ACATGTCCTG CTCTGCCGGT GCCCTCCTGC CCCAAAGGGT CAGTCCTGAC CAAGAGTTAC 
ACGCCCCCTG CTGGTTGCTG CCCTTCCATA CCACCCCAGT GCACTTGTGA CTTTGGTGCC 
TGCCAGAAGC CCCACTGCCC AAGCGGACAG CACCCCGTTC CACTCAGTCA GGCCAGCGGT 
CAGCCAGGAG ACTGCTGTGA TGTCTATGAG TGCCAGAAAG TCTCTCCTAT GTGTGACTTC 
GAAGGGAAAA AGTTCTCTGA AGGAGAGGTG TATCGCATGG ACAGCTGCTG GCTGTGTCAG 
TGCCGTGGAG GAATCTCCTT CTGCTCCAAG GCAGAGTGTG CTGAGCTAGA ATGTAAAAAC 
GTCTACACTC CTGAGGGCGA GTGCTGCCCC GTCTGCATAG ATGTAGAGCT GTTGTCATCA 
GACAGCACAA AGGCCAGTTG TTGGGTGAAT AATAAGCTGC GAGCCCATGA GGAGCAGTGG 
AAAGAGGATG ACTGCACCTT CTGTCAGTGT GTGGATGGGG ATCCTCACTG TACAGCCATG 
GCCTGCAAAC AGAGCTGCCA AAACCCTGTG AAGATCCCTG GAGAGTGTTG TCCCTTCTGT 
GAAGAACCTT CTTATGAGAC AATTAGCCCC ATTCTATGTC CACCCCTGGA AAACTGCTCC 
TTGTCAGTGA ATGACTGTCC TCATGGCTTC CAACAAGACA AAAATGGGTG TCTCCTCTGC 
CAGTGCCTCA ACAATGACTC CTGTCCTGAC TTGGGAAAAT ACTGTTCTCT GCAATGTCCA 
ATGGGATATG AGCGGGATGA TTTTGGCTGT CAGGTGTGTG AGTGCAGCAT TCCCACATCC 
AAGTGCCGAC CTCTGACCTG CACAAAGACC TGCCCCTACG GATATGTGAG GAACAAACAC 
GGCTGTGAAA TGTGTCGTTG TGTGAAATGT CCTCCCTTCA CCTGCGACAA GCACTGCAGC 
AATGGCTATC GACAGAACCG GAAGGGCTGC TCCATCTGCA CGTGTAAAGA AAGTAGCCAT 
GTCCCAACCA CAACCATCCC AGCTCCAACA CCCAGCTTCT GCCTCACCTC CAACGGGCGC 
CATTACGAGG AGGGCGACAG CTGGCATGAC GGCTGCCGGG ACTGCTTCTG CCACTCGGGA 
CGGGAGATGT GCGTGCTCAT ATCTTGCCCC ATGCCCAGCT GCCCTCATCC GGTTGTCAAG 
CCTGATCATT GTTGTCCAAC CTGTGAGGAT GAGTCCGGCA GTGGCCAGCC AGAGGTGGTT 
TGCAGAGCAC CTGGGGGGGA ATTTTACGTG GAGGGGGAGA CCTGGAACCT GGACGAGTGC 
ACACGCTGCA CCTGCAGGAA GGGTCGCGTC CTGTGCGACA CTGAAGTGTG TCCCCCGGCT 
CTCTGTCAGA CGCCCATTAG GAACAAGGAC ACATGTTGCC ATGTGTGCCA AGAGGAGACG 
CTAAGCTCTT TGCTGCCAGT AAACAGCAGC CAGCAGCAGT ACTGCATGAC CAGTGAGGGG 
GACGTGCTGC TAGCGGGGGA CTCCTGGAAG GCCAACGCCT GCACTAGCTG CACCTGCAAC 
AACGGCACCA TTCAGTGTTT CTCTCAGCGC TGCCCGGCTG CCAACTGCAG GGTACCGGTT 
TTACGCAAGG GCCAGTGCTG TCCACATTGT CTCGATGTGA CGACGTCGGT GCCAGTAATA 
GTGTCCACCA GCGCTCCAAA ATCAGTGATC ACCACTACAA GGGGACACAC ACCTTGGATC 
ACCACAGTCA CTGTCCCACT TGTCATTGCT GATACAGATG AGCCAGGTTT GGGTGAGAAT 
TACCCCAGTC AAACAGAGAT GGCCCTTGTT TACCAATCAG CAGCCTGGAT CCTGGCAGGA 
ATCCTCCTGG CCATCATCAT CTTCCTCATT GTGGCGATTC TCATCAACAA GAAGAAGAAG 
TGGGTGCAGA TGTCTTGCTA CAGTGCACCA AAGAAGACAG TGATCCTGAA GAACCACATA 
AACAAGAACT CCGTCATCTA CATGGAGCCC TCCAAGGAGA ACAAATTCCA GAACGTAAAA 
AAAGACGGCG GCATCATCCA CTTCTCTCCA GACATGAGCT CCCCTGACAA GATGACCATC 
CCCAGGGCCA AGCTGGGCTT GAACGACTGG ACGCCCCGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017268294.1
CDS	334..3135
Translation	MFGKGSVISLSVLILVRALLAHECTPCNLRTCPLKAPRGACEGGRSVARDPCGCCEQCARLEWETCGGPDWTHGYCALGLSCASVNATGAATVPETGVCKVLPDHPNAGLEDERCPLMTGCDRSGGQCVCDARHSCLGSFTYPNREACLKASKSDGRKHEHRERHREKYVGPANPECMFSGCNLTAEGCICESQSCHHHFAYINRSQCQEAAEQLRCAGVTCPALPVPSCPKGSVLTKSYTPPAGCCPSIPPQCTCDFGACQKPHCPSGQHPVPLSQASGQPGDCCDVYECQKVSPMCDFEGKKFSEGEVYRMDSCWLCQCRGGISFCSKAECAELECKNVYTPEGECCPVCIDVELLSSDSTKASCWVNNKLRAHEEQWKEDDCTFCQCVDGDPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETISPILCPPLENCSLSVNDCPHGFQQDKNGCLLCQCLNNDSCPDLGKYCSLQCPMGYERDDFGCQVCECSIPTSKCRPLTCTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCSNGYRQNRKGCSICTCKESSHVPTTTIPAPTPSFCLTSNGRHYEEGDSWHDGCRDCFCHSGREMCVLISCPMPSCPHPVVKPDHCCPTCEDESGSGQPEVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQTPIRNKDTCCHVCQEETLSSLLPVNSSQQQYCMTSEGDVLLAGDSWKANACTSCTCNNGTIQCFSQRCPAANCRVPVLRKGQCCPHCLDVTTSVPVIVSTSAPKSVITTTRGHTPWITTVTVPLVIADTDEPGLGENYPSQTEMALVYQSAAWILAGILLAIIIFLIVAILINKKKKWVQMSCYSAPKKTVILKNHINKNSVIYMEPSKENKFQNVKKDGGIIHFSPDMSSPDKMTIPRAKLGLNDWTPR

Target ORF information:

RefSeq Version	XM_017412805.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017412805.1

ORF Insert Sequence:

ATGTTCGGCA AAGGCTCCGT TATCTCTCTG AGCGTCCTCA TTCTGGTGCG CGCGCTCCTG 
GCTCACGAGT GCACGCCTTG TAACCTCCGG ACGTGTCCCC TCAAAGCCCC GCGCGGTGCC 
TGCGAGGGCG GCCGGAGTGT GGCGAGGGAC CCCTGCGGAT GCTGCGAGCA GTGCGCGCGG 
CTGGAGTGGG AAACGTGCGG CGGTCCGGAC TGGACGCACG GATACTGCGC CCTGGGGCTG 
AGCTGCGCCT CCGTCAACGC GACAGGAGCT GCCACCGTCC CTGAAACTGG AGTGTGTAAA 
GTGCTTCCTG ACCATCCTAA TGCAGGTCTC GAGGATGAAC GCTGTCCACT GATGACAGGC 
TGCGACAGAT CGGGGGGTCA GTGTGTGTGC GATGCACGTC ACTCCTGCTT GGGATCTTTT 
ACCTATCCGA ACCGAGAAGC CTGTTTGAAG GCCAGCAAGT CAGATGGTCG AAAGCACGAG 
CACCGGGAGA GACACAGGGA GAAGTACGTC GGACCGGCCA ATCCAGAGTG CATGTTCTCC 
GGGTGTAACC TGACTGCTGA GGGCTGCATT TGCGAGTCTC AGAGCTGCCA CCACCACTTT 
GCCTACATCA ATCGCAGCCA GTGCCAGGAA GCAGCAGAAC AGCTGAGGTG TGCTGGGGTG 
ACATGTCCTG CTCTGCCGGT GCCCTCCTGC CCCAAAGGGT CAGTCCTGAC CAAGAGTTAC 
ACGCCCCCTG CTGGTTGCTG CCCTTCCATA CCACCCCAGT GCACTTGTGA CTTTGGTGCC 
TGCCAGAAGC CCCACTGCCC AAGCGGACAG CACCCCGTTC CACTCAGTCA GGCCAGCGGT 
CAGCCAGGAG ACTGCTGTGA TGTCTATGAG TGCCAGAAAG TCTCTCCTAT GTGTGACTTC 
GAAGGGAAAA AGTTCTCTGA AGGAGAGGTG TATCGCATGG ACAGCTGCTG GCTGTGTCAG 
TGCCGTGGAG GAATCTCCTT CTGCTCCAAG GCAGAGTGTG CTGAGCTAGA ATGTAAAAAC 
GTCTACACTC CTGAGGGCGA GTGCTGCCCC GTCTGCATAG ATGTAGAGCT GTTGTCATCA 
GACAGCACAA AGGCCAGTTG TTGGGTGAAT AATAAGCTGC GAGCCCATGA GGAGCAGTGG 
AAAGAGGATG ACTGCACCTT CTGTCAGTGT GTGGATGGGG ATCCTCACTG TACAGCCATG 
GCCTGCAAAC AGAGCTGCCA AAACCCTGTG AAGATCCCTG GAGAGTGTTG TCCCTTCTGT 
GAAGAACCTT CTTATGAGAC AATTAGCCCC ATTCTATGTC CACCCCTGGA AAACTGCTCC 
TTGTCAGTGA ATGACTGTCC TCATGGCTTC CAACAAGACA AAAATGGGTG TCTCCTCTGC 
CAGTGCCTCA ACAATGACTC CTGTCCTGAC TTGGGAAAAT ACTGTTCTCT GCAATGTCCA 
ATGGGATATG AGCGGGATGA TTTTGGCTGT CAGGTGTGTG AGTGCAGCAT TCCCACATCC 
AAGTGCCGAC CTCTGACCTG CACAAAGACC TGCCCCTACG GATATGTGAG GAACAAACAC 
GGCTGTGAAA TGTGTCGTTG TGTGAAATGT CCTCCCTTCA CCTGCGACAA GCACTGCAGC 
AATGGCTATC GACAGAACCG GAAGGGCTGC TCCATCTGCA CGTGTAAAGA AAGTAGCCAT 
GTCCCAACCA CAACCATCCC AGCTCCAACA CCCAGCTTCT GCCTCACCTC CAACGGGCGC 
CATTACGAGG AGGGCGACAG CTGGCATGAC GGCTGCCGGG ACTGCTTCTG CCACTCGGGA 
CGGGAGATGT GCGTGCTCAT ATCTTGCCCC ATGCCCAGCT GCCCTCATCC GGTTGTCAAG 
CCTGATCATT GTTGTCCAAC CTGTGAGGAT GAGTCCGGCA GTGGCCAGCC AGAGGTGGTT 
TGCAGAGCAC CTGGGGGGGA ATTTTACGTG GAGGGGGAGA CCTGGAACCT GGACGAGTGC 
ACACGCTGCA CCTGCAGGAA GGGTCGCGTC CTGTGCGACA CTGAAGTGTG TCCCCCGGCT 
CTCTGTCAGA CGCCCATTAG GAACAAGGAC ACATGTTGCC ATGTGTGCCA AGAGGAGACG 
CTAAGCTCTT TGCTGCCAGT AAACAGCAGC CAGCAGCAGT ACTGCATGAC CAGTGAGGGG 
GACGTGCTGC TAGCGGGGGA CTCCTGGAAG GCCAACGCCT GCACTAGCTG CACCTGCAAC 
AACGGCACCA TTCAGTGTTT CTCTCAGCGC TGCCCGGCTG CCAACTGCAG GGTACCGGTT 
TTACGCAAGG GCCAGTGCTG TCCACATTGT CTCGATGTGA CGACGTCGGT GCCAGTAATA 
GTGTCCACCA GCGCTCCAAA ATCAGTGATC ACCACTACAA GGGGACACAC ACCTTGGATC 
ACCACAGTCA CTGTCCCACT TGTCATTGCT GATACAGATG AGCCAGGTTT GGGTGAGAAT 
TACCCCAGTC AAACAGAGAT GGCCCTTGTT TACCAATCAG CAGCCTGGAT CCTGGCAGGA 
ATCCTCCTGG CCATCATCAT CTTCCTCATT GTGGCGATTC TCATCAACAA GAAGAAGAAG 
TGGGTGCAGA TGTCTTGCTA CAGTGCACCA AAGAAGACAG TGATCCTGAA GAACCACATA 
AACAAGAACT CCGTCATCTA CATGGAGCCC TCCAAGGAGA ACAAATTCCA GAACGTAAAA 
AAAGACGGCG GCATCATCCA CTTCTCTCCA GACATGAGCT CCCCTGACAA GATGACCATC 
CCCAGGGCCA AGCTGGGCTT GAACGACTGG ACGCCCCGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crim1 ( XM_017412805.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017268294.1 Kryptolebias marmoratus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Price & Availability↑

CloneID	OKr00921
Clone ID Related Accession (Same CDS sequence)	XM_017412805.1 , XM_017412805.2
Accession Version	XM_017412805.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094241.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017412805.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGGCA AAGGCTCCGT TATCTCTCTG AGCGTCCTCA TTCTGGTGCG CGCGCTCCTG 
GCTCACGAGT GCACGCCTTG TAACCTCCGG ACGTGTCCCC TCAAAGCCCC GCGCGGTGCC 
TGCGAGGGCG GCCGGAGTGT GGCGAGGGAC CCCTGCGGAT GCTGCGAGCA GTGCGCGCGG 
CTGGAGTGGG AAACGTGCGG CGGTCCGGAC TGGACGCACG GATACTGCGC CCTGGGGCTG 
AGCTGCGCCT CCGTCAACGC GACAGGAGCT GCCACCGTCC CTGAAACTGG AGTGTGTAAA 
GTGCTTCCTG ACCATCCTAA TGCAGGTCTC GAGGATGAAC GCTGTCCACT GATGACAGGC 
TGCGACAGAT CGGGGGGTCA GTGTGTGTGC GATGCACGTC ACTCCTGCTT GGGATCTTTT 
ACCTATCCGA ACCGAGAAGC CTGTTTGAAG GCCAGCAAGT CAGATGGTCG AAAGCACGAG 
CACCGGGAGA GACACAGGGA GAAGTACGTC GGACCGGCCA ATCCAGAGTG CATGTTCTCC 
GGGTGTAACC TGACTGCTGA GGGCTGCATT TGCGAGTCTC AGAGCTGCCA CCACCACTTT 
GCCTACATCA ATCGCAGCCA GTGCCAGGAA GCAGCAGAAC AGCTGAGGTG TGCTGGGGTG 
ACATGTCCTG CTCTGCCGGT GCCCTCCTGC CCCAAAGGGT CAGTCCTGAC CAAGAGTTAC 
ACGCCCCCTG CTGGTTGCTG CCCTTCCATA CCACCCCAGT GCACTTGTGA CTTTGGTGCC 
TGCCAGAAGC CCCACTGCCC AAGCGGACAG CACCCCGTTC CACTCAGTCA GGCCAGCGGT 
CAGCCAGGAG ACTGCTGTGA TGTCTATGAG TGCCAGAAAG TCTCTCCTAT GTGTGACTTC 
GAAGGGAAAA AGTTCTCTGA AGGAGAGGTG TATCGCATGG ACAGCTGCTG GCTGTGTCAG 
TGCCGTGGAG GAATCTCCTT CTGCTCCAAG GCAGAGTGTG CTGAGCTAGA ATGTAAAAAC 
GTCTACACTC CTGAGGGCGA GTGCTGCCCC GTCTGCATAG ATGTAGAGCT GTTGTCATCA 
GACAGCACAA AGGCCAGTTG TTGGGTGAAT AATAAGCTGC GAGCCCATGA GGAGCAGTGG 
AAAGAGGATG ACTGCACCTT CTGTCAGTGT GTGGATGGGG ATCCTCACTG TACAGCCATG 
GCCTGCAAAC AGAGCTGCCA AAACCCTGTG AAGATCCCTG GAGAGTGTTG TCCCTTCTGT 
GAAGAACCTT CTTATGAGAC AATTAGCCCC ATTCTATGTC CACCCCTGGA AAACTGCTCC 
TTGTCAGTGA ATGACTGTCC TCATGGCTTC CAACAAGACA AAAATGGGTG TCTCCTCTGC 
CAGTGCCTCA ACAATGACTC CTGTCCTGAC TTGGGAAAAT ACTGTTCTCT GCAATGTCCA 
ATGGGATATG AGCGGGATGA TTTTGGCTGT CAGGTGTGTG AGTGCAGCAT TCCCACATCC 
AAGTGCCGAC CTCTGACCTG CACAAAGACC TGCCCCTACG GATATGTGAG GAACAAACAC 
GGCTGTGAAA TGTGTCGTTG TGTGAAATGT CCTCCCTTCA CCTGCGACAA GCACTGCAGC 
AATGGCTATC GACAGAACCG GAAGGGCTGC TCCATCTGCA CGTGTAAAGA AAGTAGCCAT 
GTCCCAACCA CAACCATCCC AGCTCCAACA CCCAGCTTCT GCCTCACCTC CAACGGGCGC 
CATTACGAGG AGGGCGACAG CTGGCATGAC GGCTGCCGGG ACTGCTTCTG CCACTCGGGA 
CGGGAGATGT GCGTGCTCAT ATCTTGCCCC ATGCCCAGCT GCCCTCATCC GGTTGTCAAG 
CCTGATCATT GTTGTCCAAC CTGTGAGGAT GAGTCCGGCA GTGGCCAGCC AGAGGTGGTT 
TGCAGAGCAC CTGGGGGGGA ATTTTACGTG GAGGGGGAGA CCTGGAACCT GGACGAGTGC 
ACACGCTGCA CCTGCAGGAA GGGTCGCGTC CTGTGCGACA CTGAAGTGTG TCCCCCGGCT 
CTCTGTCAGA CGCCCATTAG GAACAAGGAC ACATGTTGCC ATGTGTGCCA AGAGGAGACG 
CTAAGCTCTT TGCTGCCAGT AAACAGCAGC CAGCAGCAGT ACTGCATGAC CAGTGAGGGG 
GACGTGCTGC TAGCGGGGGA CTCCTGGAAG GCCAACGCCT GCACTAGCTG CACCTGCAAC 
AACGGCACCA TTCAGTGTTT CTCTCAGCGC TGCCCGGCTG CCAACTGCAG GGTACCGGTT 
TTACGCAAGG GCCAGTGCTG TCCACATTGT CTCGATGTGA CGACGTCGGT GCCAGTAATA 
GTGTCCACCA GCGCTCCAAA ATCAGTGATC ACCACTACAA GGGGACACAC ACCTTGGATC 
ACCACAGTCA CTGTCCCACT TGTCATTGCT GATACAGATG AGCCAGGTTT GGGTGAGAAT 
TACCCCAGTC AAACAGAGAT GGCCCTTGTT TACCAATCAG CAGCCTGGAT CCTGGCAGGA 
ATCCTCCTGG CCATCATCAT CTTCCTCATT GTGGCGATTC TCATCAACAA GAAGAAGAAG 
TGGGTGCAGA TGTCTTGCTA CAGTGCACCA AAGAAGACAG TGATCCTGAA GAACCACATA 
AACAAGAACT CCGTCATCTA CATGGAGCCC TCCAAGGAGA ACAAATTCCA GAACGTAAAA 
AAAGACGGCG GCATCATCCA CTTCTCTCCA GACATGAGCT CCCCTGACAA GATGACCATC 
CCCAGGGCCA AGCTGGGCTT GAACGACTGG ACGCCCCGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017268294.1
CDS	334..3135
Translation	MFGKGSVISLSVLILVRALLAHECTPCNLRTCPLKAPRGACEGGRSVARDPCGCCEQCARLEWETCGGPDWTHGYCALGLSCASVNATGAATVPETGVCKVLPDHPNAGLEDERCPLMTGCDRSGGQCVCDARHSCLGSFTYPNREACLKASKSDGRKHEHRERHREKYVGPANPECMFSGCNLTAEGCICESQSCHHHFAYINRSQCQEAAEQLRCAGVTCPALPVPSCPKGSVLTKSYTPPAGCCPSIPPQCTCDFGACQKPHCPSGQHPVPLSQASGQPGDCCDVYECQKVSPMCDFEGKKFSEGEVYRMDSCWLCQCRGGISFCSKAECAELECKNVYTPEGECCPVCIDVELLSSDSTKASCWVNNKLRAHEEQWKEDDCTFCQCVDGDPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETISPILCPPLENCSLSVNDCPHGFQQDKNGCLLCQCLNNDSCPDLGKYCSLQCPMGYERDDFGCQVCECSIPTSKCRPLTCTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCSNGYRQNRKGCSICTCKESSHVPTTTIPAPTPSFCLTSNGRHYEEGDSWHDGCRDCFCHSGREMCVLISCPMPSCPHPVVKPDHCCPTCEDESGSGQPEVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQTPIRNKDTCCHVCQEETLSSLLPVNSSQQQYCMTSEGDVLLAGDSWKANACTSCTCNNGTIQCFSQRCPAANCRVPVLRKGQCCPHCLDVTTSVPVIVSTSAPKSVITTTRGHTPWITTVTVPLVIADTDEPGLGENYPSQTEMALVYQSAAWILAGILLAIIIFLIVAILINKKKKWVQMSCYSAPKKTVILKNHINKNSVIYMEPSKENKFQNVKKDGGIIHFSPDMSSPDKMTIPRAKLGLNDWTPR

Target ORF information:

RefSeq Version	XM_017412805.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017412805.2

ORF Insert Sequence:

ATGTTCGGCA AAGGCTCCGT TATCTCTCTG AGCGTCCTCA TTCTGGTGCG CGCGCTCCTG 
GCTCACGAGT GCACGCCTTG TAACCTCCGG ACGTGTCCCC TCAAAGCCCC GCGCGGTGCC 
TGCGAGGGCG GCCGGAGTGT GGCGAGGGAC CCCTGCGGAT GCTGCGAGCA GTGCGCGCGG 
CTGGAGTGGG AAACGTGCGG CGGTCCGGAC TGGACGCACG GATACTGCGC CCTGGGGCTG 
AGCTGCGCCT CCGTCAACGC GACAGGAGCT GCCACCGTCC CTGAAACTGG AGTGTGTAAA 
GTGCTTCCTG ACCATCCTAA TGCAGGTCTC GAGGATGAAC GCTGTCCACT GATGACAGGC 
TGCGACAGAT CGGGGGGTCA GTGTGTGTGC GATGCACGTC ACTCCTGCTT GGGATCTTTT 
ACCTATCCGA ACCGAGAAGC CTGTTTGAAG GCCAGCAAGT CAGATGGTCG AAAGCACGAG 
CACCGGGAGA GACACAGGGA GAAGTACGTC GGACCGGCCA ATCCAGAGTG CATGTTCTCC 
GGGTGTAACC TGACTGCTGA GGGCTGCATT TGCGAGTCTC AGAGCTGCCA CCACCACTTT 
GCCTACATCA ATCGCAGCCA GTGCCAGGAA GCAGCAGAAC AGCTGAGGTG TGCTGGGGTG 
ACATGTCCTG CTCTGCCGGT GCCCTCCTGC CCCAAAGGGT CAGTCCTGAC CAAGAGTTAC 
ACGCCCCCTG CTGGTTGCTG CCCTTCCATA CCACCCCAGT GCACTTGTGA CTTTGGTGCC 
TGCCAGAAGC CCCACTGCCC AAGCGGACAG CACCCCGTTC CACTCAGTCA GGCCAGCGGT 
CAGCCAGGAG ACTGCTGTGA TGTCTATGAG TGCCAGAAAG TCTCTCCTAT GTGTGACTTC 
GAAGGGAAAA AGTTCTCTGA AGGAGAGGTG TATCGCATGG ACAGCTGCTG GCTGTGTCAG 
TGCCGTGGAG GAATCTCCTT CTGCTCCAAG GCAGAGTGTG CTGAGCTAGA ATGTAAAAAC 
GTCTACACTC CTGAGGGCGA GTGCTGCCCC GTCTGCATAG ATGTAGAGCT GTTGTCATCA 
GACAGCACAA AGGCCAGTTG TTGGGTGAAT AATAAGCTGC GAGCCCATGA GGAGCAGTGG 
AAAGAGGATG ACTGCACCTT CTGTCAGTGT GTGGATGGGG ATCCTCACTG TACAGCCATG 
GCCTGCAAAC AGAGCTGCCA AAACCCTGTG AAGATCCCTG GAGAGTGTTG TCCCTTCTGT 
GAAGAACCTT CTTATGAGAC AATTAGCCCC ATTCTATGTC CACCCCTGGA AAACTGCTCC 
TTGTCAGTGA ATGACTGTCC TCATGGCTTC CAACAAGACA AAAATGGGTG TCTCCTCTGC 
CAGTGCCTCA ACAATGACTC CTGTCCTGAC TTGGGAAAAT ACTGTTCTCT GCAATGTCCA 
ATGGGATATG AGCGGGATGA TTTTGGCTGT CAGGTGTGTG AGTGCAGCAT TCCCACATCC 
AAGTGCCGAC CTCTGACCTG CACAAAGACC TGCCCCTACG GATATGTGAG GAACAAACAC 
GGCTGTGAAA TGTGTCGTTG TGTGAAATGT CCTCCCTTCA CCTGCGACAA GCACTGCAGC 
AATGGCTATC GACAGAACCG GAAGGGCTGC TCCATCTGCA CGTGTAAAGA AAGTAGCCAT 
GTCCCAACCA CAACCATCCC AGCTCCAACA CCCAGCTTCT GCCTCACCTC CAACGGGCGC 
CATTACGAGG AGGGCGACAG CTGGCATGAC GGCTGCCGGG ACTGCTTCTG CCACTCGGGA 
CGGGAGATGT GCGTGCTCAT ATCTTGCCCC ATGCCCAGCT GCCCTCATCC GGTTGTCAAG 
CCTGATCATT GTTGTCCAAC CTGTGAGGAT GAGTCCGGCA GTGGCCAGCC AGAGGTGGTT 
TGCAGAGCAC CTGGGGGGGA ATTTTACGTG GAGGGGGAGA CCTGGAACCT GGACGAGTGC 
ACACGCTGCA CCTGCAGGAA GGGTCGCGTC CTGTGCGACA CTGAAGTGTG TCCCCCGGCT 
CTCTGTCAGA CGCCCATTAG GAACAAGGAC ACATGTTGCC ATGTGTGCCA AGAGGAGACG 
CTAAGCTCTT TGCTGCCAGT AAACAGCAGC CAGCAGCAGT ACTGCATGAC CAGTGAGGGG 
GACGTGCTGC TAGCGGGGGA CTCCTGGAAG GCCAACGCCT GCACTAGCTG CACCTGCAAC 
AACGGCACCA TTCAGTGTTT CTCTCAGCGC TGCCCGGCTG CCAACTGCAG GGTACCGGTT 
TTACGCAAGG GCCAGTGCTG TCCACATTGT CTCGATGTGA CGACGTCGGT GCCAGTAATA 
GTGTCCACCA GCGCTCCAAA ATCAGTGATC ACCACTACAA GGGGACACAC ACCTTGGATC 
ACCACAGTCA CTGTCCCACT TGTCATTGCT GATACAGATG AGCCAGGTTT GGGTGAGAAT 
TACCCCAGTC AAACAGAGAT GGCCCTTGTT TACCAATCAG CAGCCTGGAT CCTGGCAGGA 
ATCCTCCTGG CCATCATCAT CTTCCTCATT GTGGCGATTC TCATCAACAA GAAGAAGAAG 
TGGGTGCAGA TGTCTTGCTA CAGTGCACCA AAGAAGACAG TGATCCTGAA GAACCACATA 
AACAAGAACT CCGTCATCTA CATGGAGCCC TCCAAGGAGA ACAAATTCCA GAACGTAAAA 
AAAGACGGCG GCATCATCCA CTTCTCTCCA GACATGAGCT CCCCTGACAA GATGACCATC 
CCCAGGGCCA AGCTGGGCTT GAACGACTGG ACGCCCCGCT AA