VPS4B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS4B
Entrez Gene ID	9525
Full Name	vacuolar protein sorting 4 homolog B
Synonyms	MIG1,SKD1,SKD1B,VPS4-2
General protein information	Preferred Names vacuolar protein sorting 4 homolog B Names vacuolar protein sorting-associated protein 4B cell migration-inducing 1 cell migration-inducing gene 1 protein suppressor of K(+) transport growth defect 1 suppressor of K+ transport defect 1 vacuolar protein sorting 4B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18q21.33
Summary	The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq, Jul 2008].
Disorder MIM:	609983

mRNA and Protein(s)

mRNA	Protein	Name
XM_006722582.2	XP_006722645.1	vacuolar protein sorting-associated protein 4B isoform X2
NM_004869.3	NP_004860.2	vacuolar protein sorting-associated protein 4B
XM_011526268.3	XP_011524570.1	vacuolar protein sorting-associated protein 4B isoform X1
NM_004869.4	NP_004860.2	vacuolar protein sorting-associated protein 4B
XM_011526268.2	XP_011524570.1	vacuolar protein sorting-associated protein 4B isoform X1
XM_006722582.3	XP_006722645.1	vacuolar protein sorting-associated protein 4B isoform X2

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os01g0141100	NP_001041983.1
Homo sapiens (human)	VPS4B	NP_004860.2
Canis lupus familiaris (dog)	VPS4B	XP_005615402.1
Eremothecium gossypii	AGOS_AEL265W	NP_984595.1
Xenopus tropicalis (tropical clawed frog)	vps4b	NP_001011154.1
Rattus norvegicus (Norway rat)	Vps4b	NP_001020887.1
Gallus gallus (chicken)	VPS4B	NP_001006378.2
Caenorhabditis elegans (roundworm)	vps-4	NP_490816.4
Schizosaccharomyces pombe (fission yeast)	vps4	NP_593086.1
Pan troglodytes (chimpanzee)	VPS4B	XP_523954.2
	VPS4B	XP_002801004.1
Bos taurus (cattle)	VPS4B	NP_001069624.1
Neurospora crassa	NCU06942	XP_958048.1
Arabidopsis thaliana (thale cress)	SKD1	NP_180328.1
Mus musculus (house mouse)	Vps4b	NP_033216.2
Danio rerio (zebrafish)	vps4b	NP_957200.1
Saccharomyces cerevisiae (baker's yeast)	VPS4	NP_015499.1
Kluyveromyces lactis	KLLA0B10846g	XP_452011.1
Magnaporthe oryzae (rice blast fungus)	MGG_07916	XP_003713158.1

Gene Ontology
Bibliography

Related articles in PubMed

Vacuolar Protein Sorting 4B (VPS4B) Regulates Apoptosis of Chondrocytes via p38 Mitogen-Activated Protein Kinases (MAPK) in Osteoarthritis.
Xu L, Zhai L, Ge Q, Liu Z, Tao R
Inflammation40(6)1924-1932(2017 Dec)

A splicing mutation in VPS4B causes dentin dysplasia I.
Yang Q, Chen D, Xiong F, Chen D, Liu C, Liu Y, Yu Q, Xiong J, Liu J, Li K, Zhao L, Ye Y, Zhou H, Hu L, Tian Z, Shang X, Zhang L, Wei X, Zhou W, Li D, Zhang W, Xu X
Journal of medical genetics53(9)624-33(2016 Sep)

Cell adhesion down-regulates the expression of vacuolar protein sorting 4B (VPS4B) and contributes to drug resistance in multiple myeloma cells.
Tang J, Ji L, Wang Y, Huang Y, Yin H, He Y, Liu J, Miao X, Wu Y, Xu X, He S, Cheng C
International journal of hematology102(1)25-34(2015 Jul)

Distinct mechanisms of recognizing endosomal sorting complex required for transport III (ESCRT-III) protein IST1 by different microtubule interacting and trafficking (MIT) domains.
Guo EZ, Xu Z
The Journal of biological chemistry290(13)8396-408(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of vps4b in zebrafish recapitulated the reduction of tooth size.
Title: A splicing mutation in VPS4B causes dentin dysplasia I.

Our findings support a role for VPS4B in MM cell proliferation, adhesion, and drug resistance, and pave the way for a novel therapeutic approach targeting this molecule.
Title: Cell adhesion down-regulates the expression of vacuolar protein sorting 4B (VPS4B) and contributes to drug resistance in multiple myeloma cells.

Crystal structures of three molecular complexes reveal that IST1 binds to the MIT domains of VPS4 and LIP5.
Title: Distinct mechanisms of recognizing endosomal sorting complex required for transport III (ESCRT-III) protein IST1 by different microtubule interacting and trafficking (MIT) domains.

ESCRT-III protein CHMP5 inhibits LIP5-mediated VPS4 activation by inducing a moderate conformational change within LIP5.
Title: A novel mechanism of regulating the ATPase VPS4 by its cofactor LIP5 and the endosomal sorting complex required for transport (ESCRT)-III protein CHMP5.

Lack of ALG-2, ALIX or Vps4B each prevents shedding, and repair of the injured cell membrane
Title: Mechanism of Ca??-triggered ESCRT assembly and regulation of cell membrane repair.

The following VPS4B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS4B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17423	NM_004869.4 Latest version!	Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu51362	XM_006722582.3 Latest version!	Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu62917	XM_011526268.3 Latest version!	Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu17423	NM_004869.3	Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu62917	XM_011526268.2	Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu51362	XM_006722582.2	Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS4B ( NM_004869.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004860.2 Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), mRNA.

Price & Availability↑

CloneID	OHu17423
Clone ID Related Accession (Same CDS sequence)	NM_004869.3 , NM_004869.4
Accession Version	NM_004869.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-11
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 4B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA306069.1 and AF195514.1. On Apr 7, 2006 this sequence version replaced NM_004869.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF195514.1, SRR1660803.10147.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCATCCA CTTCGCCCAA CCTCCAGAAA GCGATAGATC TGGCTAGCAA AGCAGCGCAA 
GAAGACAAGG CTGGGAACTA CGAAGAAGCC CTTCAGCTCT ATCAGCATGC TGTGCAGTAT 
TTTCTTCATG TCGTTAAATA TGAAGCACAG GGTGATAAAG CCAAGCAAAG TATCAGGGCA 
AAGTGTACAG AATATCTTGA TAGAGCAGAA AAACTAAAGG AGTACCTGAA AAATAAAGAG 
AAAAAAGCAC AGAAGCCAGT GAAAGAAGGA CAGCCGAGTC CAGCAGATGA GAAGGGGAAT 
GACAGTGATG GGGAAGGAGA ATCTGATGAT CCTGAAAAAA AGAAACTACA GAATCAACTT 
CAAGGTGCCA TTGTTATAGA ACGACCAAAT GTGAAATGGA GTGACGTTGC TGGACTTGAA 
GGAGCCAAAG AAGCACTGAA AGAGGCTGTG ATACTGCCTA TTAAATTTCC TCATCTTTTT 
ACAGGCAAGA GAACACCTTG GAGGGGAATC CTATTATTTG GGCCGCCTGG AACAGGAAAG 
TCCTACTTAG CCAAAGCTGT AGCAACAGAA GCCAACAACT CAACATTTTT TTCAATATCT 
TCCTCTGATC TTGTTTCTAA GTGGCTAGGT GAAAGTGAAA AACTGGTTAA GAATTTATTC 
CAACTTGCCA GAGAGAACAA GCCCTCCATT ATCTTCATTG ATGAAATTGA TTCTCTCTGT 
GGTTCAAGAA GTGAAAATGA AAGTGAAGCC GCACGTAGAA TTAAGACGGA GTTCCTAGTG 
CAAATGCAAG GGGTTGGTGT AGACAATGAT GGAATTTTGG TTCTGGGAGC TACAAATATA 
CCCTGGGTTC TGGATTCTGC CATTAGGCGA AGATTTGAGA AACGAATTTA TATTCCCTTG 
CCGGAACCCC ATGCCCGAGC AGCAATGTTT AAACTGCACC TAGGGACCAC TCAGAACAGT 
CTCACGGAAG CAGACTTTCG GGAACTTGGG AGGAAAACAG ATGGTTATTC AGGGGCAGAT 
ATAAGTATCA TTGTACGTGA TGCCCTTATG CAGCCTGTTA GGAAAGTACA GTCAGCTACT 
CATTTTAAAA AGGTTCGCGG ACCTTCCCGA GCTGATCCTA ACCATCTTGT AGATGATCTG 
CTAACACCTT GCTCTCCAGG TGACCCTGGT GCCATTGAAA TGACATGGAT GGATGTCCCT 
GGAGATAAAC TTTTGGAGCC AGTTGTTTCC ATGTCGGATA TGTTGCGGTC ACTATCTAAC 
ACAAAACCTA CAGTCAATGA ACATGACTTG TTGAAATTAA AGAAGTTTAC AGAAGATTTT 
GGTCAAGAAG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004860.2
CDS	261..1595
Translation	MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG

Target ORF information:

RefSeq Version	NM_004869.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004869.3

ORF Insert Sequence:

ATGTCATCCA CTTCGCCCAA CCTCCAGAAA GCGATAGATC TGGCTAGCAA AGCAGCGCAA 
GAAGACAAGG CTGGGAACTA CGAAGAAGCC CTTCAGCTCT ATCAGCATGC TGTGCAGTAT 
TTTCTTCATG TCGTTAAATA TGAAGCACAG GGTGATAAAG CCAAGCAAAG TATCAGGGCA 
AAGTGTACAG AATATCTTGA TAGAGCAGAA AAACTAAAGG AGTACCTGAA AAATAAAGAG 
AAAAAAGCAC AGAAGCCAGT GAAAGAAGGA CAGCCGAGTC CAGCAGATGA GAAGGGGAAT 
GACAGTGATG GGGAAGGAGA ATCTGATGAT CCTGAAAAAA AGAAACTACA GAATCAACTT 
CAAGGTGCCA TTGTTATAGA ACGACCAAAT GTGAAATGGA GTGACGTTGC TGGACTTGAA 
GGAGCCAAAG AAGCACTGAA AGAGGCTGTG ATACTGCCTA TTAAATTTCC TCATCTTTTT 
ACAGGCAAGA GAACACCTTG GAGGGGAATC CTATTATTTG GGCCGCCTGG AACAGGAAAG 
TCCTACTTAG CCAAAGCTGT AGCAACAGAA GCCAACAACT CAACATTTTT TTCAATATCT 
TCCTCTGATC TTGTTTCTAA GTGGCTAGGT GAAAGTGAAA AACTGGTTAA GAATTTATTC 
CAACTTGCCA GAGAGAACAA GCCCTCCATT ATCTTCATTG ATGAAATTGA TTCTCTCTGT 
GGTTCAAGAA GTGAAAATGA AAGTGAAGCC GCACGTAGAA TTAAGACGGA GTTCCTAGTG 
CAAATGCAAG GGGTTGGTGT AGACAATGAT GGAATTTTGG TTCTGGGAGC TACAAATATA 
CCCTGGGTTC TGGATTCTGC CATTAGGCGA AGATTTGAGA AACGAATTTA TATTCCCTTG 
CCGGAACCCC ATGCCCGAGC AGCAATGTTT AAACTGCACC TAGGGACCAC TCAGAACAGT 
CTCACGGAAG CAGACTTTCG GGAACTTGGG AGGAAAACAG ATGGTTATTC AGGGGCAGAT 
ATAAGTATCA TTGTACGTGA TGCCCTTATG CAGCCTGTTA GGAAAGTACA GTCAGCTACT 
CATTTTAAAA AGGTTCGCGG ACCTTCCCGA GCTGATCCTA ACCATCTTGT AGATGATCTG 
CTAACACCTT GCTCTCCAGG TGACCCTGGT GCCATTGAAA TGACATGGAT GGATGTCCCT 
GGAGATAAAC TTTTGGAGCC AGTTGTTTCC ATGTCGGATA TGTTGCGGTC ACTATCTAAC 
ACAAAACCTA CAGTCAATGA ACATGACTTG TTGAAATTAA AGAAGTTTAC AGAAGATTTT 
GGTCAAGAAG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS4B ( NM_004869.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004860.2 Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), mRNA.

Price & Availability↑

CloneID	OHu17423
Clone ID Related Accession (Same CDS sequence)	NM_004869.3 , NM_004869.4
Accession Version	NM_004869.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 4B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA306069.1 and AF195514.1. On Nov 23, 2018 this sequence version replaced NM_004869.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF195514.1, SRR1660803.10147.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000238497.10/ ENSP00000238497.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCATCCA CTTCGCCCAA CCTCCAGAAA GCGATAGATC TGGCTAGCAA AGCAGCGCAA 
GAAGACAAGG CTGGGAACTA CGAAGAAGCC CTTCAGCTCT ATCAGCATGC TGTGCAGTAT 
TTTCTTCATG TCGTTAAATA TGAAGCACAG GGTGATAAAG CCAAGCAAAG TATCAGGGCA 
AAGTGTACAG AATATCTTGA TAGAGCAGAA AAACTAAAGG AGTACCTGAA AAATAAAGAG 
AAAAAAGCAC AGAAGCCAGT GAAAGAAGGA CAGCCGAGTC CAGCAGATGA GAAGGGGAAT 
GACAGTGATG GGGAAGGAGA ATCTGATGAT CCTGAAAAAA AGAAACTACA GAATCAACTT 
CAAGGTGCCA TTGTTATAGA ACGACCAAAT GTGAAATGGA GTGACGTTGC TGGACTTGAA 
GGAGCCAAAG AAGCACTGAA AGAGGCTGTG ATACTGCCTA TTAAATTTCC TCATCTTTTT 
ACAGGCAAGA GAACACCTTG GAGGGGAATC CTATTATTTG GGCCGCCTGG AACAGGAAAG 
TCCTACTTAG CCAAAGCTGT AGCAACAGAA GCCAACAACT CAACATTTTT TTCAATATCT 
TCCTCTGATC TTGTTTCTAA GTGGCTAGGT GAAAGTGAAA AACTGGTTAA GAATTTATTC 
CAACTTGCCA GAGAGAACAA GCCCTCCATT ATCTTCATTG ATGAAATTGA TTCTCTCTGT 
GGTTCAAGAA GTGAAAATGA AAGTGAAGCC GCACGTAGAA TTAAGACGGA GTTCCTAGTG 
CAAATGCAAG GGGTTGGTGT AGACAATGAT GGAATTTTGG TTCTGGGAGC TACAAATATA 
CCCTGGGTTC TGGATTCTGC CATTAGGCGA AGATTTGAGA AACGAATTTA TATTCCCTTG 
CCGGAACCCC ATGCCCGAGC AGCAATGTTT AAACTGCACC TAGGGACCAC TCAGAACAGT 
CTCACGGAAG CAGACTTTCG GGAACTTGGG AGGAAAACAG ATGGTTATTC AGGGGCAGAT 
ATAAGTATCA TTGTACGTGA TGCCCTTATG CAGCCTGTTA GGAAAGTACA GTCAGCTACT 
CATTTTAAAA AGGTTCGCGG ACCTTCCCGA GCTGATCCTA ACCATCTTGT AGATGATCTG 
CTAACACCTT GCTCTCCAGG TGACCCTGGT GCCATTGAAA TGACATGGAT GGATGTCCCT 
GGAGATAAAC TTTTGGAGCC AGTTGTTTCC ATGTCGGATA TGTTGCGGTC ACTATCTAAC 
ACAAAACCTA CAGTCAATGA ACATGACTTG TTGAAATTAA AGAAGTTTAC AGAAGATTTT 
GGTCAAGAAG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004860.2
CDS	218..1552
Translation	MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG

Target ORF information:

RefSeq Version	NM_004869.4
Organism	Homo sapiens(human)
Definition	Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004869.4

ORF Insert Sequence:

ATGTCATCCA CTTCGCCCAA CCTCCAGAAA GCGATAGATC TGGCTAGCAA AGCAGCGCAA 
GAAGACAAGG CTGGGAACTA CGAAGAAGCC CTTCAGCTCT ATCAGCATGC TGTGCAGTAT 
TTTCTTCATG TCGTTAAATA TGAAGCACAG GGTGATAAAG CCAAGCAAAG TATCAGGGCA 
AAGTGTACAG AATATCTTGA TAGAGCAGAA AAACTAAAGG AGTACCTGAA AAATAAAGAG 
AAAAAAGCAC AGAAGCCAGT GAAAGAAGGA CAGCCGAGTC CAGCAGATGA GAAGGGGAAT 
GACAGTGATG GGGAAGGAGA ATCTGATGAT CCTGAAAAAA AGAAACTACA GAATCAACTT 
CAAGGTGCCA TTGTTATAGA ACGACCAAAT GTGAAATGGA GTGACGTTGC TGGACTTGAA 
GGAGCCAAAG AAGCACTGAA AGAGGCTGTG ATACTGCCTA TTAAATTTCC TCATCTTTTT 
ACAGGCAAGA GAACACCTTG GAGGGGAATC CTATTATTTG GGCCGCCTGG AACAGGAAAG 
TCCTACTTAG CCAAAGCTGT AGCAACAGAA GCCAACAACT CAACATTTTT TTCAATATCT 
TCCTCTGATC TTGTTTCTAA GTGGCTAGGT GAAAGTGAAA AACTGGTTAA GAATTTATTC 
CAACTTGCCA GAGAGAACAA GCCCTCCATT ATCTTCATTG ATGAAATTGA TTCTCTCTGT 
GGTTCAAGAA GTGAAAATGA AAGTGAAGCC GCACGTAGAA TTAAGACGGA GTTCCTAGTG 
CAAATGCAAG GGGTTGGTGT AGACAATGAT GGAATTTTGG TTCTGGGAGC TACAAATATA 
CCCTGGGTTC TGGATTCTGC CATTAGGCGA AGATTTGAGA AACGAATTTA TATTCCCTTG 
CCGGAACCCC ATGCCCGAGC AGCAATGTTT AAACTGCACC TAGGGACCAC TCAGAACAGT 
CTCACGGAAG CAGACTTTCG GGAACTTGGG AGGAAAACAG ATGGTTATTC AGGGGCAGAT 
ATAAGTATCA TTGTACGTGA TGCCCTTATG CAGCCTGTTA GGAAAGTACA GTCAGCTACT 
CATTTTAAAA AGGTTCGCGG ACCTTCCCGA GCTGATCCTA ACCATCTTGT AGATGATCTG 
CTAACACCTT GCTCTCCAGG TGACCCTGGT GCCATTGAAA TGACATGGAT GGATGTCCCT 
GGAGATAAAC TTTTGGAGCC AGTTGTTTCC ATGTCGGATA TGTTGCGGTC ACTATCTAAC 
ACAAAACCTA CAGTCAATGA ACATGACTTG TTGAAATTAA AGAAGTTTAC AGAAGATTTT 
GGTCAAGAAG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS4B ( XM_006722582.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722645.1 Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu51362
Clone ID Related Accession (Same CDS sequence)	XM_006722582.2 , XM_006722582.3
Accession Version	XM_006722582.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722582.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGGG GTGCCATTGT TATAGAACGA CCAAATGTGA AATGGAGTGA CGTTGCTGGA 
CTTGAAGGAG CCAAAGAAGC ACTGAAAGAG GCTGTGATAC TGCCTATTAA ATTTCCTCAT 
CTTTTTACAG GCAAGAGAAC ACCTTGGAGG GGAATCCTAT TATTTGGGCC GCCTGGAACA 
GGAAAGTCCT ACTTAGCCAA AGCTGTAGCA ACAGAAGCCA ACAACTCAAC ATTTTTTTCA 
ATATCTTCCT CTGATCTTGT TTCTAAGTGG CTAGGTGAAA GTGAAAAACT GGTTAAGAAT 
TTATTCCAAC TTGCCAGAGA GAACAAGCCC TCCATTATCT TCATTGATGA AATTGATTCT 
CTCTGTGGTT CAAGAAGTGA AAATGAAAGT GAAGCCGCAC GTAGAATTAA GACGGAGTTC 
CTAGTGCAAA TGCAAGGGGT TGGTGTAGAC AATGATGGAA TTTTGGTTCT GGGAGCTACA 
AATATACCCT GGGTTCTGGA TTCTGCCATT AGGCGAAGAT TTGAGAAACG AATTTATATT 
CCCTTGCCGG AACCCCATGC CCGAGCAGCA ATGTTTAAAC TGCACCTAGG GACCACTCAG 
AACAGTCTCA CGGAAGCAGA CTTTCGGGAA CTTGGGAGGA AAACAGATGG TTATTCAGGG 
GCAGATATAA GTATCATTGT ACGTGATGCC CTTATGCAGC CTGTTAGGAA AGTACAGTCA 
GCTACTCATT TTAAAAAGGT TCGCGGACCT TCCCGAGCTG ATCCTAACCA TCTTGTAGAT 
GATCTGCTAA CACCTTGCTC TCCAGGTGAC CCTGGTGCCA TTGAAATGAC ATGGATGGAT 
GTCCCTGGAG ATAAACTTTT GGAGCCAGTT GTTTCCATGT CGGATATGTT GCGGTCACTA 
TCTAACACAA AACCTACAGT CAATGAACAT GACTTGTTGA AATTAAAGAA GTTTACAGAA 
GATTTTGGTC AAGAAGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722645.1
CDS	491..1471
Translation	MRRGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG

Target ORF information:

RefSeq Version	XM_006722582.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722582.2

ORF Insert Sequence:

ATGAGAAGGG GTGCCATTGT TATAGAACGA CCAAATGTGA AATGGAGTGA CGTTGCTGGA 
CTTGAAGGAG CCAAAGAAGC ACTGAAAGAG GCTGTGATAC TGCCTATTAA ATTTCCTCAT 
CTTTTTACAG GCAAGAGAAC ACCTTGGAGG GGAATCCTAT TATTTGGGCC GCCTGGAACA 
GGAAAGTCCT ACTTAGCCAA AGCTGTAGCA ACAGAAGCCA ACAACTCAAC ATTTTTTTCA 
ATATCTTCCT CTGATCTTGT TTCTAAGTGG CTAGGTGAAA GTGAAAAACT GGTTAAGAAT 
TTATTCCAAC TTGCCAGAGA GAACAAGCCC TCCATTATCT TCATTGATGA AATTGATTCT 
CTCTGTGGTT CAAGAAGTGA AAATGAAAGT GAAGCCGCAC GTAGAATTAA GACGGAGTTC 
CTAGTGCAAA TGCAAGGGGT TGGTGTAGAC AATGATGGAA TTTTGGTTCT GGGAGCTACA 
AATATACCCT GGGTTCTGGA TTCTGCCATT AGGCGAAGAT TTGAGAAACG AATTTATATT 
CCCTTGCCGG AACCCCATGC CCGAGCAGCA ATGTTTAAAC TGCACCTAGG GACCACTCAG 
AACAGTCTCA CGGAAGCAGA CTTTCGGGAA CTTGGGAGGA AAACAGATGG TTATTCAGGG 
GCAGATATAA GTATCATTGT ACGTGATGCC CTTATGCAGC CTGTTAGGAA AGTACAGTCA 
GCTACTCATT TTAAAAAGGT TCGCGGACCT TCCCGAGCTG ATCCTAACCA TCTTGTAGAT 
GATCTGCTAA CACCTTGCTC TCCAGGTGAC CCTGGTGCCA TTGAAATGAC ATGGATGGAT 
GTCCCTGGAG ATAAACTTTT GGAGCCAGTT GTTTCCATGT CGGATATGTT GCGGTCACTA 
TCTAACACAA AACCTACAGT CAATGAACAT GACTTGTTGA AATTAAAGAA GTTTACAGAA 
GATTTTGGTC AAGAAGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS4B ( XM_006722582.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722645.1 Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu51362
Clone ID Related Accession (Same CDS sequence)	XM_006722582.2 , XM_006722582.3
Accession Version	XM_006722582.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006722582.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGGG GTGCCATTGT TATAGAACGA CCAAATGTGA AATGGAGTGA CGTTGCTGGA 
CTTGAAGGAG CCAAAGAAGC ACTGAAAGAG GCTGTGATAC TGCCTATTAA ATTTCCTCAT 
CTTTTTACAG GCAAGAGAAC ACCTTGGAGG GGAATCCTAT TATTTGGGCC GCCTGGAACA 
GGAAAGTCCT ACTTAGCCAA AGCTGTAGCA ACAGAAGCCA ACAACTCAAC ATTTTTTTCA 
ATATCTTCCT CTGATCTTGT TTCTAAGTGG CTAGGTGAAA GTGAAAAACT GGTTAAGAAT 
TTATTCCAAC TTGCCAGAGA GAACAAGCCC TCCATTATCT TCATTGATGA AATTGATTCT 
CTCTGTGGTT CAAGAAGTGA AAATGAAAGT GAAGCCGCAC GTAGAATTAA GACGGAGTTC 
CTAGTGCAAA TGCAAGGGGT TGGTGTAGAC AATGATGGAA TTTTGGTTCT GGGAGCTACA 
AATATACCCT GGGTTCTGGA TTCTGCCATT AGGCGAAGAT TTGAGAAACG AATTTATATT 
CCCTTGCCGG AACCCCATGC CCGAGCAGCA ATGTTTAAAC TGCACCTAGG GACCACTCAG 
AACAGTCTCA CGGAAGCAGA CTTTCGGGAA CTTGGGAGGA AAACAGATGG TTATTCAGGG 
GCAGATATAA GTATCATTGT ACGTGATGCC CTTATGCAGC CTGTTAGGAA AGTACAGTCA 
GCTACTCATT TTAAAAAGGT TCGCGGACCT TCCCGAGCTG ATCCTAACCA TCTTGTAGAT 
GATCTGCTAA CACCTTGCTC TCCAGGTGAC CCTGGTGCCA TTGAAATGAC ATGGATGGAT 
GTCCCTGGAG ATAAACTTTT GGAGCCAGTT GTTTCCATGT CGGATATGTT GCGGTCACTA 
TCTAACACAA AACCTACAGT CAATGAACAT GACTTGTTGA AATTAAAGAA GTTTACAGAA 
GATTTTGGTC AAGAAGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722645.1
CDS	491..1471
Translation	MRRGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG

Target ORF information:

RefSeq Version	XM_006722582.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722582.3

ORF Insert Sequence:

ATGAGAAGGG GTGCCATTGT TATAGAACGA CCAAATGTGA AATGGAGTGA CGTTGCTGGA 
CTTGAAGGAG CCAAAGAAGC ACTGAAAGAG GCTGTGATAC TGCCTATTAA ATTTCCTCAT 
CTTTTTACAG GCAAGAGAAC ACCTTGGAGG GGAATCCTAT TATTTGGGCC GCCTGGAACA 
GGAAAGTCCT ACTTAGCCAA AGCTGTAGCA ACAGAAGCCA ACAACTCAAC ATTTTTTTCA 
ATATCTTCCT CTGATCTTGT TTCTAAGTGG CTAGGTGAAA GTGAAAAACT GGTTAAGAAT 
TTATTCCAAC TTGCCAGAGA GAACAAGCCC TCCATTATCT TCATTGATGA AATTGATTCT 
CTCTGTGGTT CAAGAAGTGA AAATGAAAGT GAAGCCGCAC GTAGAATTAA GACGGAGTTC 
CTAGTGCAAA TGCAAGGGGT TGGTGTAGAC AATGATGGAA TTTTGGTTCT GGGAGCTACA 
AATATACCCT GGGTTCTGGA TTCTGCCATT AGGCGAAGAT TTGAGAAACG AATTTATATT 
CCCTTGCCGG AACCCCATGC CCGAGCAGCA ATGTTTAAAC TGCACCTAGG GACCACTCAG 
AACAGTCTCA CGGAAGCAGA CTTTCGGGAA CTTGGGAGGA AAACAGATGG TTATTCAGGG 
GCAGATATAA GTATCATTGT ACGTGATGCC CTTATGCAGC CTGTTAGGAA AGTACAGTCA 
GCTACTCATT TTAAAAAGGT TCGCGGACCT TCCCGAGCTG ATCCTAACCA TCTTGTAGAT 
GATCTGCTAA CACCTTGCTC TCCAGGTGAC CCTGGTGCCA TTGAAATGAC ATGGATGGAT 
GTCCCTGGAG ATAAACTTTT GGAGCCAGTT GTTTCCATGT CGGATATGTT GCGGTCACTA 
TCTAACACAA AACCTACAGT CAATGAACAT GACTTGTTGA AATTAAAGAA GTTTACAGAA 
GATTTTGGTC AAGAAGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS4B ( XM_011526268.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524570.1 Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu62917
Clone ID Related Accession (Same CDS sequence)	XM_011526268.3 , XM_011526268.2
Accession Version	XM_011526268.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011526268.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCCA CTTCGCCCAA CCTCCAGAAA GCGATAGATC TGGCTAGCAA AGCAGCGCAA 
GAAGACAAGG CTGGGAACTA CGAAGAAGCC CTTCAGCTCT ATCAGCATGC TGTGCAGTAT 
TTTCTTCATG TCGTTAAATA TGAAGCACAG GGTGATAAAG CCAAGCAAAG TATCAGGGCA 
AAGTGTACAG AATATCTTGA TAGAGCAGAA AAACTAAAGG AGTACCTGAA AAATAAAGAG 
AAAAAAGCAC AGAAGCCAGT GAAAGAAGGA CAGCCGAGTC CAGCAGATGA GAAGGGGAAT 
GACAGTGATG GGGAAGGAGA ATCTGATGAT CCTGAAAAAA AGAAACTACA GAATCAACTT 
CAAGGTGCCA TTGTTATAGA ACGACCAAAT GTGAAATGGA GTGACGTTGC TGGACTTGAA 
GGAGCCAAAG AAGCACTGAA AGAGGCTGTG ATACTGCCTA TTAAATTTCC TCATCTTTTT 
ACAGGCAAGA GAACACCTTG GAGGGGAATC CTATTATTTG GGCCGCCTGG AACAGGAAAG 
TCCTACTTAG CCAAAGCTGT AGCAACAGAA GCCAACAACT CAACATTTTT TTCAATATCT 
TCCTCTGATC TTGTTTCTAA GTGGCTAGGT GAAAGTGAAA AACTGGTTAA GAATTTATTC 
CAACTTGCCA GAGAGAACAA GCCCTCCATT ATCTTCATTG ATGAAATTGA TTCTCTCTGT 
GGTTCAAGAA GTGAAAATGA AAGTGAAGCC GCACGTAGAA TTAAGACGGA GTTCCTAGTG 
CAAATGCAAG GGGTTGGTGT AGACAATGAT GGAATTTTGG TTCTGGGAGC TACAAATATA 
CCCTGGGTTC TGGATTCTGC CATTAGGCGA AGATTTGAGA AACGAATTTA TATTCCCTTG 
CCGGAACCCC ATGCCCGAGC AGCAATGTTT AAACTGCACC TAGGGACCAC TCAGAACAGT 
CTCACGGAAG CAGACTTTCG GGAACTTGGG AGGAAAACAG ATGGTTATTC AGGGGCAGAT 
ATAAGTATCA TTGTACGTGA TGCCCTTATG CAGCCTGTTA GGAAAGTACA GTCAGCTACT 
CATTTTAAAA AGGTTCGCGG ACCTTCCCGA GCTGATCCTA ACCATCTTGT AGATGATCTG 
CTAACACCTT GCTCTCCAGG TGACCCTGGT GCCATTGAAA TGACATGGAT GGATGTCCCT 
GGAGATAAAC TTTTGGAGCC AGTTGTTTCC ATGTTTCAGA TCTTCAAAGA CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524570.1
CDS	237..1493
Translation	MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMFQIFKDL

Target ORF information:

RefSeq Version	XM_011526268.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526268.3

ORF Insert Sequence:

ATGTCATCCA CTTCGCCCAA CCTCCAGAAA GCGATAGATC TGGCTAGCAA AGCAGCGCAA 
GAAGACAAGG CTGGGAACTA CGAAGAAGCC CTTCAGCTCT ATCAGCATGC TGTGCAGTAT 
TTTCTTCATG TCGTTAAATA TGAAGCACAG GGTGATAAAG CCAAGCAAAG TATCAGGGCA 
AAGTGTACAG AATATCTTGA TAGAGCAGAA AAACTAAAGG AGTACCTGAA AAATAAAGAG 
AAAAAAGCAC AGAAGCCAGT GAAAGAAGGA CAGCCGAGTC CAGCAGATGA GAAGGGGAAT 
GACAGTGATG GGGAAGGAGA ATCTGATGAT CCTGAAAAAA AGAAACTACA GAATCAACTT 
CAAGGTGCCA TTGTTATAGA ACGACCAAAT GTGAAATGGA GTGACGTTGC TGGACTTGAA 
GGAGCCAAAG AAGCACTGAA AGAGGCTGTG ATACTGCCTA TTAAATTTCC TCATCTTTTT 
ACAGGCAAGA GAACACCTTG GAGGGGAATC CTATTATTTG GGCCGCCTGG AACAGGAAAG 
TCCTACTTAG CCAAAGCTGT AGCAACAGAA GCCAACAACT CAACATTTTT TTCAATATCT 
TCCTCTGATC TTGTTTCTAA GTGGCTAGGT GAAAGTGAAA AACTGGTTAA GAATTTATTC 
CAACTTGCCA GAGAGAACAA GCCCTCCATT ATCTTCATTG ATGAAATTGA TTCTCTCTGT 
GGTTCAAGAA GTGAAAATGA AAGTGAAGCC GCACGTAGAA TTAAGACGGA GTTCCTAGTG 
CAAATGCAAG GGGTTGGTGT AGACAATGAT GGAATTTTGG TTCTGGGAGC TACAAATATA 
CCCTGGGTTC TGGATTCTGC CATTAGGCGA AGATTTGAGA AACGAATTTA TATTCCCTTG 
CCGGAACCCC ATGCCCGAGC AGCAATGTTT AAACTGCACC TAGGGACCAC TCAGAACAGT 
CTCACGGAAG CAGACTTTCG GGAACTTGGG AGGAAAACAG ATGGTTATTC AGGGGCAGAT 
ATAAGTATCA TTGTACGTGA TGCCCTTATG CAGCCTGTTA GGAAAGTACA GTCAGCTACT 
CATTTTAAAA AGGTTCGCGG ACCTTCCCGA GCTGATCCTA ACCATCTTGT AGATGATCTG 
CTAACACCTT GCTCTCCAGG TGACCCTGGT GCCATTGAAA TGACATGGAT GGATGTCCCT 
GGAGATAAAC TTTTGGAGCC AGTTGTTTCC ATGTTTCAGA TCTTCAAAGA CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS4B ( XM_011526268.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524570.1 Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu62917
Clone ID Related Accession (Same CDS sequence)	XM_011526268.3 , XM_011526268.2
Accession Version	XM_011526268.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011526268.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCCA CTTCGCCCAA CCTCCAGAAA GCGATAGATC TGGCTAGCAA AGCAGCGCAA 
GAAGACAAGG CTGGGAACTA CGAAGAAGCC CTTCAGCTCT ATCAGCATGC TGTGCAGTAT 
TTTCTTCATG TCGTTAAATA TGAAGCACAG GGTGATAAAG CCAAGCAAAG TATCAGGGCA 
AAGTGTACAG AATATCTTGA TAGAGCAGAA AAACTAAAGG AGTACCTGAA AAATAAAGAG 
AAAAAAGCAC AGAAGCCAGT GAAAGAAGGA CAGCCGAGTC CAGCAGATGA GAAGGGGAAT 
GACAGTGATG GGGAAGGAGA ATCTGATGAT CCTGAAAAAA AGAAACTACA GAATCAACTT 
CAAGGTGCCA TTGTTATAGA ACGACCAAAT GTGAAATGGA GTGACGTTGC TGGACTTGAA 
GGAGCCAAAG AAGCACTGAA AGAGGCTGTG ATACTGCCTA TTAAATTTCC TCATCTTTTT 
ACAGGCAAGA GAACACCTTG GAGGGGAATC CTATTATTTG GGCCGCCTGG AACAGGAAAG 
TCCTACTTAG CCAAAGCTGT AGCAACAGAA GCCAACAACT CAACATTTTT TTCAATATCT 
TCCTCTGATC TTGTTTCTAA GTGGCTAGGT GAAAGTGAAA AACTGGTTAA GAATTTATTC 
CAACTTGCCA GAGAGAACAA GCCCTCCATT ATCTTCATTG ATGAAATTGA TTCTCTCTGT 
GGTTCAAGAA GTGAAAATGA AAGTGAAGCC GCACGTAGAA TTAAGACGGA GTTCCTAGTG 
CAAATGCAAG GGGTTGGTGT AGACAATGAT GGAATTTTGG TTCTGGGAGC TACAAATATA 
CCCTGGGTTC TGGATTCTGC CATTAGGCGA AGATTTGAGA AACGAATTTA TATTCCCTTG 
CCGGAACCCC ATGCCCGAGC AGCAATGTTT AAACTGCACC TAGGGACCAC TCAGAACAGT 
CTCACGGAAG CAGACTTTCG GGAACTTGGG AGGAAAACAG ATGGTTATTC AGGGGCAGAT 
ATAAGTATCA TTGTACGTGA TGCCCTTATG CAGCCTGTTA GGAAAGTACA GTCAGCTACT 
CATTTTAAAA AGGTTCGCGG ACCTTCCCGA GCTGATCCTA ACCATCTTGT AGATGATCTG 
CTAACACCTT GCTCTCCAGG TGACCCTGGT GCCATTGAAA TGACATGGAT GGATGTCCCT 
GGAGATAAAC TTTTGGAGCC AGTTGTTTCC ATGTTTCAGA TCTTCAAAGA CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524570.1
CDS	261..1517
Translation	MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMFQIFKDL

Target ORF information:

RefSeq Version	XM_011526268.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vacuolar protein sorting 4 homolog B (VPS4B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526268.2

ORF Insert Sequence:

ATGTCATCCA CTTCGCCCAA CCTCCAGAAA GCGATAGATC TGGCTAGCAA AGCAGCGCAA 
GAAGACAAGG CTGGGAACTA CGAAGAAGCC CTTCAGCTCT ATCAGCATGC TGTGCAGTAT 
TTTCTTCATG TCGTTAAATA TGAAGCACAG GGTGATAAAG CCAAGCAAAG TATCAGGGCA 
AAGTGTACAG AATATCTTGA TAGAGCAGAA AAACTAAAGG AGTACCTGAA AAATAAAGAG 
AAAAAAGCAC AGAAGCCAGT GAAAGAAGGA CAGCCGAGTC CAGCAGATGA GAAGGGGAAT 
GACAGTGATG GGGAAGGAGA ATCTGATGAT CCTGAAAAAA AGAAACTACA GAATCAACTT 
CAAGGTGCCA TTGTTATAGA ACGACCAAAT GTGAAATGGA GTGACGTTGC TGGACTTGAA 
GGAGCCAAAG AAGCACTGAA AGAGGCTGTG ATACTGCCTA TTAAATTTCC TCATCTTTTT 
ACAGGCAAGA GAACACCTTG GAGGGGAATC CTATTATTTG GGCCGCCTGG AACAGGAAAG 
TCCTACTTAG CCAAAGCTGT AGCAACAGAA GCCAACAACT CAACATTTTT TTCAATATCT 
TCCTCTGATC TTGTTTCTAA GTGGCTAGGT GAAAGTGAAA AACTGGTTAA GAATTTATTC 
CAACTTGCCA GAGAGAACAA GCCCTCCATT ATCTTCATTG ATGAAATTGA TTCTCTCTGT 
GGTTCAAGAA GTGAAAATGA AAGTGAAGCC GCACGTAGAA TTAAGACGGA GTTCCTAGTG 
CAAATGCAAG GGGTTGGTGT AGACAATGAT GGAATTTTGG TTCTGGGAGC TACAAATATA 
CCCTGGGTTC TGGATTCTGC CATTAGGCGA AGATTTGAGA AACGAATTTA TATTCCCTTG 
CCGGAACCCC ATGCCCGAGC AGCAATGTTT AAACTGCACC TAGGGACCAC TCAGAACAGT 
CTCACGGAAG CAGACTTTCG GGAACTTGGG AGGAAAACAG ATGGTTATTC AGGGGCAGAT 
ATAAGTATCA TTGTACGTGA TGCCCTTATG CAGCCTGTTA GGAAAGTACA GTCAGCTACT 
CATTTTAAAA AGGTTCGCGG ACCTTCCCGA GCTGATCCTA ACCATCTTGT AGATGATCTG 
CTAACACCTT GCTCTCCAGG TGACCCTGGT GCCATTGAAA TGACATGGAT GGATGTCCCT 
GGAGATAAAC TTTTGGAGCC AGTTGTTTCC ATGTTTCAGA TCTTCAAAGA CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Vacuolar Protein Sorting 4B (VPS4B) Regulates Apoptosis of Chondrocytes via p38 Mitogen-Activated Protein Kinases (MAPK) in Osteoarthritis.
Inflammation40(6)1924-1932(2017 Dec)
Xu L,Zhai L,Ge Q,Liu Z,Tao R

A splicing mutation in VPS4B causes dentin dysplasia I.
Journal of medical genetics53(9)624-33(2016 Sep)
Yang Q,Chen D,Xiong F,Chen D,Liu C,Liu Y,Yu Q,Xiong J,Liu J,Li K,Zhao L,Ye Y,Zhou H,Hu L,Tian Z,Shang X,Zhang L,Wei X,Zhou W,Li D,Zhang W,Xu X

Cell adhesion down-regulates the expression of vacuolar protein sorting 4B (VPS4B) and contributes to drug resistance in multiple myeloma cells.
International journal of hematology102(1)25-34(2015 Jul)
Tang J,Ji L,Wang Y,Huang Y,Yin H,He Y,Liu J,Miao X,Wu Y,Xu X,He S,Cheng C

Distinct mechanisms of recognizing endosomal sorting complex required for transport III (ESCRT-III) protein IST1 by different microtubule interacting and trafficking (MIT) domains.
The Journal of biological chemistry290(13)8396-408(2015 Mar)
Guo EZ,Xu Z

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VPS4B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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