UBE3B gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011538959.1	XP_011537261.1	ubiquitin-protein ligase E3B isoform X1
XM_011538959.2	XP_011537261.1	ubiquitin-protein ligase E3B isoform X1
XM_024449269.1	XP_024305037.1	ubiquitin-protein ligase E3B isoform X5
NM_183415.3	NP_904324.1	ubiquitin-protein ligase E3B isoform 1
NM_130466.4	NP_569733.2	ubiquitin-protein ligase E3B isoform 1
NM_001270451.1	NP_001257380.1	ubiquitin-protein ligase E3B isoform 3
XM_005253987.2	XP_005254044.1	ubiquitin-protein ligase E3B isoform X1
XM_017020196.1	XP_016875685.1	ubiquitin-protein ligase E3B isoform X6
NM_130466.3	NP_569733.2	ubiquitin-protein ligase E3B isoform 1
NM_183415.2	NP_904324.1	ubiquitin-protein ligase E3B isoform 1
NM_001270449.1	NP_001257378.1	ubiquitin-protein ligase E3B isoform 3
XM_006719681.3	XP_006719744.1	ubiquitin-protein ligase E3B isoform X2
XM_005253987.1	XP_005254044.1	ubiquitin-protein ligase E3B isoform X1
NM_001270450.1	NP_001257379.1	ubiquitin-protein ligase E3B isoform 3
XM_006719682.2	XP_006719745.1	ubiquitin-protein ligase E3B isoform X3
XM_011538961.1	XP_011537263.1	ubiquitin-protein ligase E3B isoform X4
XM_017020195.1	XP_016875684.1	ubiquitin-protein ligase E3B isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09547	NM_130466.4 Latest version!	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu09547	NM_183415.3 Latest version!	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu09629	NM_001270450.1 Latest version!	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu09629	NM_001270449.1 Latest version!	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu09629	NM_001270451.1 Latest version!	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu09547	XM_005253987.2 Latest version!	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu09547	XM_011538959.2 Latest version!	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu09629	XM_017020196.1 Latest version!	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu84312	XM_017020195.1 Latest version!	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu84312	XM_024449269.1 Latest version!	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu46963	XM_006719681.3 Latest version!	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu46964	XM_006719682.2 Latest version!	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu74142	XM_011538961.1 Latest version!	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu09547	XM_011538959.1	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu09547	NM_130466.3	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu09547	NM_183415.2	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu09547	XM_005253987.1	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
Hide Old Accession Versions >>

UBE3B ( NM_183415.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_904324.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu09547
Clone ID Related Accession (Same CDS sequence)	XM_011538959.1 , XM_011538959.2 , NM_183415.3 , NM_130466.4 , XM_005253987.2 , NM_130466.3 , NM_183415.2 , XM_005253987.1
Accession Version	NM_183415.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA771082.1, BC032301.1, AF251046.2, BQ889238.1 and DR760197.1. On May 31, 2019 this sequence version replaced NM_183415.2. Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR, as compared to variant 1. Variants 1 and 3 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537403.1, SRR1803612.148695.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_904324.1
CDS	259..3465
Translation	MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLESHDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCRKYVSQKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASPQNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQELDRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDITDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	NM_183415.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183415.3

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( NM_130466.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_569733.2 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09547
Clone ID Related Accession (Same CDS sequence)	XM_011538959.1 , XM_011538959.2 , NM_183415.3 , NM_130466.4 , XM_005253987.2 , NM_130466.3 , NM_183415.2 , XM_005253987.1
Accession Version	NM_130466.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA771082.1, BC144684.1, BC032301.1, AF251046.2, BQ889238.1 and DR760197.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_130466.3. Transcript Variant: This variant (1) represents the longest transcript, and encodes the longer protein (isoform 1). Variants 1 and 3 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF251046.2, SRR1660805.197067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000342494.8/ ENSP00000340596.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569733.2
CDS	604..3810
Translation	MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLESHDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCRKYVSQKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASPQNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQELDRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDITDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	NM_130466.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130466.4

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( NM_130466.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_569733.2 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 1, mRNA.

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CloneID	OHu09547
Clone ID Related Accession (Same CDS sequence)	XM_011538959.1 , XM_011538959.2 , NM_183415.3 , NM_130466.4 , XM_005253987.2 , NM_130466.3 , NM_183415.2 , XM_005253987.1
Accession Version	NM_130466.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-02
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA771082.1, BC144684.1, BC032301.1, AF251046.2, BQ889238.1 and DR760197.1. This sequence is a reference standard in the RefSeqGene project. On Jul 7, 2012 this sequence version replaced NM_130466.2. Transcript Variant: This variant (1) represents the longest transcript, and encodes the longer protein (isoform 1). Variants 1 and 3 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.197067.1, AF251046.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569733.2
CDS	615..3821
Translation	MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLESHDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCRKYVSQKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASPQNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQELDRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDITDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	NM_130466.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130466.3

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( NM_183415.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_904324.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 3, mRNA.

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CloneID	OHu09547
Clone ID Related Accession (Same CDS sequence)	XM_011538959.1 , XM_011538959.2 , NM_183415.3 , NM_130466.4 , XM_005253987.2 , NM_130466.3 , NM_183415.2 , XM_005253987.1
Accession Version	NM_183415.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA771082.1, BC032301.1, AF251046.2, BQ889238.1 and DR760197.1. On Jul 7, 2012 this sequence version replaced NM_183415.1. Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR, as compared to variant 1. Variants 1 and 3 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537403.1, SRR1803612.148695.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_904324.1
CDS	270..3476
Translation	MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLESHDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCRKYVSQKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASPQNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQELDRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDITDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	NM_183415.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183415.2

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( NM_001270451.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257380.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 6, mRNA.

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CloneID	OHu09629
Clone ID Related Accession (Same CDS sequence)	NM_001270451.1 , XM_017020196.1 , NM_001270449.1 , NM_001270450.1
Accession Version	NM_001270451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA771082.1, BC068221.1 and BC108705.1. Transcript Variant: This variant (6) uses an alternate splice site in the 5' UTR and contains an alternate 3' exon, compared to variant 1. These differences result in a distinct 3' UTR and 3' coding region and a protein (isoform 3) with a shorter and distinct C-terminus, compared to isoform 1. Variants 4-6 encode the same protein (isoform 3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC068221.1, BQ928951.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG TGCTGTGATG GGCTGTTTCC TGATTTGGTT 
TCATATGCTC CTCACAACAA CCCTGTGAGG TGGTCCGTTG GCAGAAGCTG GTATGACTGG 
CAGTTGTCTC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257380.1
CDS	282..1016
Translation	MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQCCDGLFPDLVSYAPHNNPVRWSVGRSWYDWQLSR

Target ORF information:

RefSeq Version	NM_001270451.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270451.1

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG TGCTGTGATG GGCTGTTTCC TGATTTGGTT 
TCATATGCTC CTCACAACAA CCCTGTGAGG TGGTCCGTTG GCAGAAGCTG GTATGACTGG 
CAGTTGTCTC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( NM_001270449.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257378.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 4, mRNA.

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CloneID	OHu09629
Clone ID Related Accession (Same CDS sequence)	NM_001270451.1 , XM_017020196.1 , NM_001270449.1 , NM_001270450.1
Accession Version	NM_001270449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA771082.1 and BC051266.1. Transcript Variant: This variant (4) contains an alternate 3' exon, compared to variant 1. This difference results in a distinct 3' UTR and 3' coding region and a protein (isoform 3) with a shorter and distinct C-terminus, compared to isoform 1. Variants 4-6 encode the same protein (isoform 3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051266.1, SRR1163658.359587.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG TGCTGTGATG GGCTGTTTCC TGATTTGGTT 
TCATATGCTC CTCACAACAA CCCTGTGAGG TGGTCCGTTG GCAGAAGCTG GTATGACTGG 
CAGTTGTCTC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257378.1
CDS	615..1349
Translation	MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQCCDGLFPDLVSYAPHNNPVRWSVGRSWYDWQLSR

Target ORF information:

RefSeq Version	NM_001270449.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270449.1

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG TGCTGTGATG GGCTGTTTCC TGATTTGGTT 
TCATATGCTC CTCACAACAA CCCTGTGAGG TGGTCCGTTG GCAGAAGCTG GTATGACTGG 
CAGTTGTCTC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( NM_001270450.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257379.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu09629
Clone ID Related Accession (Same CDS sequence)	NM_001270451.1 , XM_017020196.1 , NM_001270449.1 , NM_001270450.1
Accession Version	NM_001270450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA771082.1 and BC108705.1. Transcript Variant: This variant (5) uses an alternate splice site in the 5' UTR and contains an alternate 3' exon, compared to variant 1. These differences result in a distinct 3' UTR and 3' coding region and a protein (isoform 3) with a shorter and distinct C-terminus, compared to isoform 1. Variants 4-6 encode the same protein (isoform 3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108705.1, SRR1660807.245938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG TGCTGTGATG GGCTGTTTCC TGATTTGGTT 
TCATATGCTC CTCACAACAA CCCTGTGAGG TGGTCCGTTG GCAGAAGCTG GTATGACTGG 
CAGTTGTCTC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257379.1
CDS	270..1004
Translation	MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQCCDGLFPDLVSYAPHNNPVRWSVGRSWYDWQLSR

Target ORF information:

RefSeq Version	NM_001270450.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270450.1

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG TGCTGTGATG GGCTGTTTCC TGATTTGGTT 
TCATATGCTC CTCACAACAA CCCTGTGAGG TGGTCCGTTG GCAGAAGCTG GTATGACTGG 
CAGTTGTCTC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_011538959.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537261.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu09547
Clone ID Related Accession (Same CDS sequence)	XM_011538959.1 , XM_011538959.2 , NM_183415.3 , NM_130466.4 , XM_005253987.2 , NM_130466.3 , NM_183415.2 , XM_005253987.1
Accession Version	XM_011538959.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	ubiquitin-protein ligase E3B isoform X1
Comment

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011537261.1

Translation

MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLESHDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCRKYVSQKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASPQNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQELDRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDITDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_011538959.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538959.1

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_011538959.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537261.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu09547
Clone ID Related Accession (Same CDS sequence)	XM_011538959.1 , XM_011538959.2 , NM_183415.3 , NM_130466.4 , XM_005253987.2 , NM_130466.3 , NM_183415.2 , XM_005253987.1
Accession Version	XM_011538959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538959.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537261.1
CDS	377..3583
Translation	MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLESHDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCRKYVSQKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASPQNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQELDRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDITDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_011538959.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538959.2

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_005253987.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254044.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X1, mRNA.

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CloneID	OHu09547
Clone ID Related Accession (Same CDS sequence)	XM_011538959.1 , XM_011538959.2 , NM_183415.3 , NM_130466.4 , XM_005253987.2 , NM_130466.3 , NM_183415.2 , XM_005253987.1
Accession Version	XM_005253987.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253987.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254044.1
CDS	495..3701
Translation	MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLESHDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCRKYVSQKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASPQNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQELDRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDITDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_005253987.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253987.2

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_005253987.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254044.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu09547
Clone ID Related Accession (Same CDS sequence)	XM_011538959.1 , XM_011538959.2 , NM_183415.3 , NM_130466.4 , XM_005253987.2 , NM_130466.3 , NM_183415.2 , XM_005253987.1
Accession Version	XM_005253987.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	ubiquitin-protein ligase E3B isoform X1
Comment

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005254044.1

Translation

MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLESHDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCRKYVSQKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASPQNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQELDRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDITDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_005253987.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253987.1

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGACAC CGGGGACACT CTGGGCAGCG TCCTCCGGGG CTTCTTCACC 
ATCCGCAAGC GGGAGCCAGG CGGCCGCCTG CCCACCTCCT CCACCTGCTT CAACCTGCTC 
AAGCTGCCCA ACTACAGCAA GAAGAGCGTC CTCCGCGAGA AGCTGCGCTA CGCCATCAGC 
ATGAACACGG GCTTTGAACT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_017020196.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875685.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X10, mRNA.

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CloneID	OHu09629
Clone ID Related Accession (Same CDS sequence)	NM_001270451.1 , XM_017020196.1 , NM_001270449.1 , NM_001270450.1
Accession Version	XM_017020196.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG TGCTGTGATG GGCTGTTTCC TGATTTGGTT 
TCATATGCTC CTCACAACAA CCCTGTGAGG TGGTCCGTTG GCAGAAGCTG GTATGACTGG 
CAGTTGTCTC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875685.1
CDS	377..1111
Translation	MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQCCDGLFPDLVSYAPHNNPVRWSVGRSWYDWQLSR

Target ORF information:

RefSeq Version	XM_017020196.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020196.1

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG TGCTGTGATG GGCTGTTTCC TGATTTGGTT 
TCATATGCTC CTCACAACAA CCCTGTGAGG TGGTCCGTTG GCAGAAGCTG GTATGACTGG 
CAGTTGTCTC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_024449269.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305037.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X7, mRNA.

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CloneID	OHu84312
Clone ID Related Accession (Same CDS sequence)	XM_024449269.1 , XM_017020195.1
Accession Version	XM_024449269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATAA AGTTGATATC ATCCTCTGAC CAATTAAAAC ATTCTTTTCA GATATTGTTA 
ACCCGTGGCC TGGCAAGACC CCGTCCTTGT CTATCCAAAG GCACTTTAAC AGCAGCTTTT 
TCTCTAGCGT TACGCCCTGT GATTGCTGCA CAGTTCTCAG ACAATCTGAT TCGGCCGTTC 
CTCATCCACA TCATGTCTGT GCCTGCTCTG GTGACTCATC TCAGCACAGT GACCCCTGAG 
CGCCTCACTG TTTTAGAATC CCATGACATG CTTCGTAAAT TCATCATATT TTTAAGAGAC 
CAAGATCGAT GCCGTGATGT ATGTGAAAGT TTAGAAGGAT GCCATACGCT TTGTCTAATG 
GGCAACCTCC TACACTTGGG CTCCCTCAGC CCCAGAGTGT TAGAGGAGGA GACAGATGGG 
TTCGTGAGTT TGCTCACCCA GACGCTGTGC TACTGTCGGA AGTATGTGTC TCAGAAGAAG 
TCCAACCTGA CCCACTGGCA TCCTGTCCTT GGCTGGTTCT CCCAATCTGT GGATTATGGC 
CTTAACGAGT CAATGCACTT GATCACCAAA CAGCTGCAGT TCTTGTGGGG GGTGCCTCTG 
ATCCGGATCT TCTTCTGTGA CATCCTGAGC AAGAAGCTAC TGGAGAGCCA GGAGCCAGCC 
CACGCACAGC CAGCATCCCC TCAGAATGTG CTCCCAGTGA AGAGTCTCCT AAAGCGTGCT 
TTTCAAAAGT CGGCATCAGT CCGGAATATT CTCAGGCCTG TCGGGGGTAA ACGGGTCGAC 
TCTGCAGAAG TCCAGAAGGT TTGCAACATC TGTGTCCTCT ACCAGACCTC GCTGACAACT 
CTCACACAGA TTCGGCTGCA GATACTCACA GGTCTCACTT ACCTTGATGA CCTGCTTCCC 
AAACTGTGGG CATTTATCTG TGAGCTCGGG CCCCACGGAG GGTTAAAGCT CTTCTTGGAA 
TGCCTGAACA ATGACACTGA AGAGTCCAAG CAACTCTTGG CCATGCTGAT GCTGTTCTGT 
GACTGTTCGC GGCACCTCAT CACAATCCTT GATGACATTG AAGTTTATGA AGAACAGATT 
TCATTCAAAC TGGAAGAGCT GGTCACTATC TCCTCTTTCC TGAATTCTTT TGTGTTTAAG 
ATGATCTGGG ATGGAATTGT AGAGAACGCC AAGGGTGAGA CCTTGGAGCT GTTCCAGTCT 
GTCCACGGGT GGCTTATGGT GCTGTACGAG CGGGACTGCC GGCGGCGCTT CACCCCCGAG 
GACCACTGGC TGCGAAAGGA TCTCAAACCT AGCGTGCTCT TCCAAGAACT CGACAGGGAC 
AGAAAACGGG CACAGTTGAT CCTGCAGTAC ATCCCACATG TCATCCCTCA CAAAAACAGA 
GTTCTACTGT TTCGAACCAT GGTTACCAAG GAGAAGGAGA AACTGGGGCT GGTGGAAACC 
AGCTCTGCCT CCCCGCATGT CACTCACATC ACCATCCGCC GGTCCAGGAT GCTGGAGGAC 
GGCTACGAGC AGCTTAGGCA GCTCTCCCAG CACGCCATGA AGGGGGTCAT CCGTGTGAAG 
TTTGTCAATG ACCTCGGGGT GGACGAAGCA GGGATTGATC AAGACGGTGT TTTTAAGGAG 
TTCTTGGAAG AGATCATCAA GAGAGTTTTT GACCCAGCAC TCAATCTGTT CAAGACAACC 
AGTGGGGATG AGAGGCTGTA CCCCTCACCC ACATCCTACA TCCATGAGAA TTACCTGCAG 
CTCTTCGAGT TTGTGGGGAA GATGCTGGGG AAGGCTGTGT ATGAGGGAAT TGTGGTGGAC 
GTGCCATTTG CATCCTTCTT CCTGAGCCAA CTGCTTGGGC ACCACCACAG CGTCTTCTAT 
AGCTCGGTGG ATGAACTGCC TTCTCTGGAC TCCGAGTTCT ATAAAAACCT CACCTCCATC 
AAGCGCTATG ATGGGGACAT CACTGACCTG GGCCTGACGC TGTCTTACGA CGAGGACGTC 
ATGGGTCAGC TTGTTTGCCA TGAACTGATT CCTGGAGGGA AGACCATTCC TGTTACAAAT 
GAAAATAAAA TTAGCTACAT CCATCTGATG GCACATTTTC GAATGCACAC TCAAATAAAA 
AACCAAACAG CTGCCCTCAT TAGCGGATTC CGTTCCATTA TCAAACCCGA GTGGATCCGA 
ATGTTCTCAA CTCCTGAACT GCAGCGTCTC ATCTCTGGCG ACAATGCTGA GATTGATCTG 
GAAGATTTAA AGAAGCACAC AGTCTACTAC GGTGGTTTCC ATGGAAGTCA CAGAGTCATC 
ATCTGGCTCT GGGATATTCT GGCCTCCGAC TTCACACCGG ATGAGAGAGC TATGTTTCTG 
AAGTTCGTGA CCAGCTGCTC CAGACCCCCG CTCCTGGGAT TCGCCTACCT CAAGCCTCCA 
TTCTCCATCC GCTGCGTGGA GGTGTCGGAC GATCAGGACA CCGGGGACAC TCTGGGCAGC 
GTCCTCCGGG GCTTCTTCAC CATCCGCAAG CGGGAGCCAG GCGGCCGCCT GCCCACCTCC 
TCCACCTGCT TCAACCTGCT CAAGCTGCCC AACTACAGCA AGAAGAGCGT CCTCCGCGAG 
AAGCTGCGCT ACGCCATCAG CATGAACACG GGCTTTGAAC TCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305037.1
CDS	1962..4589
Translation	MNIKLISSSDQLKHSFQILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLESHDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCRKYVSQKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASPQNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQELDRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDITDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_024449269.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449269.1

ORF Insert Sequence:

ATGAATATAA AGTTGATATC ATCCTCTGAC CAATTAAAAC ATTCTTTTCA GATATTGTTA 
ACCCGTGGCC TGGCAAGACC CCGTCCTTGT CTATCCAAAG GCACTTTAAC AGCAGCTTTT 
TCTCTAGCGT TACGCCCTGT GATTGCTGCA CAGTTCTCAG ACAATCTGAT TCGGCCGTTC 
CTCATCCACA TCATGTCTGT GCCTGCTCTG GTGACTCATC TCAGCACAGT GACCCCTGAG 
CGCCTCACTG TTTTAGAATC CCATGACATG CTTCGTAAAT TCATCATATT TTTAAGAGAC 
CAAGATCGAT GCCGTGATGT ATGTGAAAGT TTAGAAGGAT GCCATACGCT TTGTCTAATG 
GGCAACCTCC TACACTTGGG CTCCCTCAGC CCCAGAGTGT TAGAGGAGGA GACAGATGGG 
TTCGTGAGTT TGCTCACCCA GACGCTGTGC TACTGTCGGA AGTATGTGTC TCAGAAGAAG 
TCCAACCTGA CCCACTGGCA TCCTGTCCTT GGCTGGTTCT CCCAATCTGT GGATTATGGC 
CTTAACGAGT CAATGCACTT GATCACCAAA CAGCTGCAGT TCTTGTGGGG GGTGCCTCTG 
ATCCGGATCT TCTTCTGTGA CATCCTGAGC AAGAAGCTAC TGGAGAGCCA GGAGCCAGCC 
CACGCACAGC CAGCATCCCC TCAGAATGTG CTCCCAGTGA AGAGTCTCCT AAAGCGTGCT 
TTTCAAAAGT CGGCATCAGT CCGGAATATT CTCAGGCCTG TCGGGGGTAA ACGGGTCGAC 
TCTGCAGAAG TCCAGAAGGT TTGCAACATC TGTGTCCTCT ACCAGACCTC GCTGACAACT 
CTCACACAGA TTCGGCTGCA GATACTCACA GGTCTCACTT ACCTTGATGA CCTGCTTCCC 
AAACTGTGGG CATTTATCTG TGAGCTCGGG CCCCACGGAG GGTTAAAGCT CTTCTTGGAA 
TGCCTGAACA ATGACACTGA AGAGTCCAAG CAACTCTTGG CCATGCTGAT GCTGTTCTGT 
GACTGTTCGC GGCACCTCAT CACAATCCTT GATGACATTG AAGTTTATGA AGAACAGATT 
TCATTCAAAC TGGAAGAGCT GGTCACTATC TCCTCTTTCC TGAATTCTTT TGTGTTTAAG 
ATGATCTGGG ATGGAATTGT AGAGAACGCC AAGGGTGAGA CCTTGGAGCT GTTCCAGTCT 
GTCCACGGGT GGCTTATGGT GCTGTACGAG CGGGACTGCC GGCGGCGCTT CACCCCCGAG 
GACCACTGGC TGCGAAAGGA TCTCAAACCT AGCGTGCTCT TCCAAGAACT CGACAGGGAC 
AGAAAACGGG CACAGTTGAT CCTGCAGTAC ATCCCACATG TCATCCCTCA CAAAAACAGA 
GTTCTACTGT TTCGAACCAT GGTTACCAAG GAGAAGGAGA AACTGGGGCT GGTGGAAACC 
AGCTCTGCCT CCCCGCATGT CACTCACATC ACCATCCGCC GGTCCAGGAT GCTGGAGGAC 
GGCTACGAGC AGCTTAGGCA GCTCTCCCAG CACGCCATGA AGGGGGTCAT CCGTGTGAAG 
TTTGTCAATG ACCTCGGGGT GGACGAAGCA GGGATTGATC AAGACGGTGT TTTTAAGGAG 
TTCTTGGAAG AGATCATCAA GAGAGTTTTT GACCCAGCAC TCAATCTGTT CAAGACAACC 
AGTGGGGATG AGAGGCTGTA CCCCTCACCC ACATCCTACA TCCATGAGAA TTACCTGCAG 
CTCTTCGAGT TTGTGGGGAA GATGCTGGGG AAGGCTGTGT ATGAGGGAAT TGTGGTGGAC 
GTGCCATTTG CATCCTTCTT CCTGAGCCAA CTGCTTGGGC ACCACCACAG CGTCTTCTAT 
AGCTCGGTGG ATGAACTGCC TTCTCTGGAC TCCGAGTTCT ATAAAAACCT CACCTCCATC 
AAGCGCTATG ATGGGGACAT CACTGACCTG GGCCTGACGC TGTCTTACGA CGAGGACGTC 
ATGGGTCAGC TTGTTTGCCA TGAACTGATT CCTGGAGGGA AGACCATTCC TGTTACAAAT 
GAAAATAAAA TTAGCTACAT CCATCTGATG GCACATTTTC GAATGCACAC TCAAATAAAA 
AACCAAACAG CTGCCCTCAT TAGCGGATTC CGTTCCATTA TCAAACCCGA GTGGATCCGA 
ATGTTCTCAA CTCCTGAACT GCAGCGTCTC ATCTCTGGCG ACAATGCTGA GATTGATCTG 
GAAGATTTAA AGAAGCACAC AGTCTACTAC GGTGGTTTCC ATGGAAGTCA CAGAGTCATC 
ATCTGGCTCT GGGATATTCT GGCCTCCGAC TTCACACCGG ATGAGAGAGC TATGTTTCTG 
AAGTTCGTGA CCAGCTGCTC CAGACCCCCG CTCCTGGGAT TCGCCTACCT CAAGCCTCCA 
TTCTCCATCC GCTGCGTGGA GGTGTCGGAC GATCAGGACA CCGGGGACAC TCTGGGCAGC 
GTCCTCCGGG GCTTCTTCAC CATCCGCAAG CGGGAGCCAG GCGGCCGCCT GCCCACCTCC 
TCCACCTGCT TCAACCTGCT CAAGCTGCCC AACTACAGCA AGAAGAGCGT CCTCCGCGAG 
AAGCTGCGCT ACGCCATCAG CATGAACACG GGCTTTGAAC TCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_017020195.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875684.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X8, mRNA.

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CloneID	OHu84312
Clone ID Related Accession (Same CDS sequence)	XM_024449269.1 , XM_017020195.1
Accession Version	XM_017020195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATAA AGTTGATATC ATCCTCTGAC CAATTAAAAC ATTCTTTTCA GATATTGTTA 
ACCCGTGGCC TGGCAAGACC CCGTCCTTGT CTATCCAAAG GCACTTTAAC AGCAGCTTTT 
TCTCTAGCGT TACGCCCTGT GATTGCTGCA CAGTTCTCAG ACAATCTGAT TCGGCCGTTC 
CTCATCCACA TCATGTCTGT GCCTGCTCTG GTGACTCATC TCAGCACAGT GACCCCTGAG 
CGCCTCACTG TTTTAGAATC CCATGACATG CTTCGTAAAT TCATCATATT TTTAAGAGAC 
CAAGATCGAT GCCGTGATGT ATGTGAAAGT TTAGAAGGAT GCCATACGCT TTGTCTAATG 
GGCAACCTCC TACACTTGGG CTCCCTCAGC CCCAGAGTGT TAGAGGAGGA GACAGATGGG 
TTCGTGAGTT TGCTCACCCA GACGCTGTGC TACTGTCGGA AGTATGTGTC TCAGAAGAAG 
TCCAACCTGA CCCACTGGCA TCCTGTCCTT GGCTGGTTCT CCCAATCTGT GGATTATGGC 
CTTAACGAGT CAATGCACTT GATCACCAAA CAGCTGCAGT TCTTGTGGGG GGTGCCTCTG 
ATCCGGATCT TCTTCTGTGA CATCCTGAGC AAGAAGCTAC TGGAGAGCCA GGAGCCAGCC 
CACGCACAGC CAGCATCCCC TCAGAATGTG CTCCCAGTGA AGAGTCTCCT AAAGCGTGCT 
TTTCAAAAGT CGGCATCAGT CCGGAATATT CTCAGGCCTG TCGGGGGTAA ACGGGTCGAC 
TCTGCAGAAG TCCAGAAGGT TTGCAACATC TGTGTCCTCT ACCAGACCTC GCTGACAACT 
CTCACACAGA TTCGGCTGCA GATACTCACA GGTCTCACTT ACCTTGATGA CCTGCTTCCC 
AAACTGTGGG CATTTATCTG TGAGCTCGGG CCCCACGGAG GGTTAAAGCT CTTCTTGGAA 
TGCCTGAACA ATGACACTGA AGAGTCCAAG CAACTCTTGG CCATGCTGAT GCTGTTCTGT 
GACTGTTCGC GGCACCTCAT CACAATCCTT GATGACATTG AAGTTTATGA AGAACAGATT 
TCATTCAAAC TGGAAGAGCT GGTCACTATC TCCTCTTTCC TGAATTCTTT TGTGTTTAAG 
ATGATCTGGG ATGGAATTGT AGAGAACGCC AAGGGTGAGA CCTTGGAGCT GTTCCAGTCT 
GTCCACGGGT GGCTTATGGT GCTGTACGAG CGGGACTGCC GGCGGCGCTT CACCCCCGAG 
GACCACTGGC TGCGAAAGGA TCTCAAACCT AGCGTGCTCT TCCAAGAACT CGACAGGGAC 
AGAAAACGGG CACAGTTGAT CCTGCAGTAC ATCCCACATG TCATCCCTCA CAAAAACAGA 
GTTCTACTGT TTCGAACCAT GGTTACCAAG GAGAAGGAGA AACTGGGGCT GGTGGAAACC 
AGCTCTGCCT CCCCGCATGT CACTCACATC ACCATCCGCC GGTCCAGGAT GCTGGAGGAC 
GGCTACGAGC AGCTTAGGCA GCTCTCCCAG CACGCCATGA AGGGGGTCAT CCGTGTGAAG 
TTTGTCAATG ACCTCGGGGT GGACGAAGCA GGGATTGATC AAGACGGTGT TTTTAAGGAG 
TTCTTGGAAG AGATCATCAA GAGAGTTTTT GACCCAGCAC TCAATCTGTT CAAGACAACC 
AGTGGGGATG AGAGGCTGTA CCCCTCACCC ACATCCTACA TCCATGAGAA TTACCTGCAG 
CTCTTCGAGT TTGTGGGGAA GATGCTGGGG AAGGCTGTGT ATGAGGGAAT TGTGGTGGAC 
GTGCCATTTG CATCCTTCTT CCTGAGCCAA CTGCTTGGGC ACCACCACAG CGTCTTCTAT 
AGCTCGGTGG ATGAACTGCC TTCTCTGGAC TCCGAGTTCT ATAAAAACCT CACCTCCATC 
AAGCGCTATG ATGGGGACAT CACTGACCTG GGCCTGACGC TGTCTTACGA CGAGGACGTC 
ATGGGTCAGC TTGTTTGCCA TGAACTGATT CCTGGAGGGA AGACCATTCC TGTTACAAAT 
GAAAATAAAA TTAGCTACAT CCATCTGATG GCACATTTTC GAATGCACAC TCAAATAAAA 
AACCAAACAG CTGCCCTCAT TAGCGGATTC CGTTCCATTA TCAAACCCGA GTGGATCCGA 
ATGTTCTCAA CTCCTGAACT GCAGCGTCTC ATCTCTGGCG ACAATGCTGA GATTGATCTG 
GAAGATTTAA AGAAGCACAC AGTCTACTAC GGTGGTTTCC ATGGAAGTCA CAGAGTCATC 
ATCTGGCTCT GGGATATTCT GGCCTCCGAC TTCACACCGG ATGAGAGAGC TATGTTTCTG 
AAGTTCGTGA CCAGCTGCTC CAGACCCCCG CTCCTGGGAT TCGCCTACCT CAAGCCTCCA 
TTCTCCATCC GCTGCGTGGA GGTGTCGGAC GATCAGGACA CCGGGGACAC TCTGGGCAGC 
GTCCTCCGGG GCTTCTTCAC CATCCGCAAG CGGGAGCCAG GCGGCCGCCT GCCCACCTCC 
TCCACCTGCT TCAACCTGCT CAAGCTGCCC AACTACAGCA AGAAGAGCGT CCTCCGCGAG 
AAGCTGCGCT ACGCCATCAG CATGAACACG GGCTTTGAAC TCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875684.1
CDS	1617..4244
Translation	MNIKLISSSDQLKHSFQILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLESHDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCRKYVSQKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASPQNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQELDRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDITDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_017020195.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020195.1

ORF Insert Sequence:

ATGAATATAA AGTTGATATC ATCCTCTGAC CAATTAAAAC ATTCTTTTCA GATATTGTTA 
ACCCGTGGCC TGGCAAGACC CCGTCCTTGT CTATCCAAAG GCACTTTAAC AGCAGCTTTT 
TCTCTAGCGT TACGCCCTGT GATTGCTGCA CAGTTCTCAG ACAATCTGAT TCGGCCGTTC 
CTCATCCACA TCATGTCTGT GCCTGCTCTG GTGACTCATC TCAGCACAGT GACCCCTGAG 
CGCCTCACTG TTTTAGAATC CCATGACATG CTTCGTAAAT TCATCATATT TTTAAGAGAC 
CAAGATCGAT GCCGTGATGT ATGTGAAAGT TTAGAAGGAT GCCATACGCT TTGTCTAATG 
GGCAACCTCC TACACTTGGG CTCCCTCAGC CCCAGAGTGT TAGAGGAGGA GACAGATGGG 
TTCGTGAGTT TGCTCACCCA GACGCTGTGC TACTGTCGGA AGTATGTGTC TCAGAAGAAG 
TCCAACCTGA CCCACTGGCA TCCTGTCCTT GGCTGGTTCT CCCAATCTGT GGATTATGGC 
CTTAACGAGT CAATGCACTT GATCACCAAA CAGCTGCAGT TCTTGTGGGG GGTGCCTCTG 
ATCCGGATCT TCTTCTGTGA CATCCTGAGC AAGAAGCTAC TGGAGAGCCA GGAGCCAGCC 
CACGCACAGC CAGCATCCCC TCAGAATGTG CTCCCAGTGA AGAGTCTCCT AAAGCGTGCT 
TTTCAAAAGT CGGCATCAGT CCGGAATATT CTCAGGCCTG TCGGGGGTAA ACGGGTCGAC 
TCTGCAGAAG TCCAGAAGGT TTGCAACATC TGTGTCCTCT ACCAGACCTC GCTGACAACT 
CTCACACAGA TTCGGCTGCA GATACTCACA GGTCTCACTT ACCTTGATGA CCTGCTTCCC 
AAACTGTGGG CATTTATCTG TGAGCTCGGG CCCCACGGAG GGTTAAAGCT CTTCTTGGAA 
TGCCTGAACA ATGACACTGA AGAGTCCAAG CAACTCTTGG CCATGCTGAT GCTGTTCTGT 
GACTGTTCGC GGCACCTCAT CACAATCCTT GATGACATTG AAGTTTATGA AGAACAGATT 
TCATTCAAAC TGGAAGAGCT GGTCACTATC TCCTCTTTCC TGAATTCTTT TGTGTTTAAG 
ATGATCTGGG ATGGAATTGT AGAGAACGCC AAGGGTGAGA CCTTGGAGCT GTTCCAGTCT 
GTCCACGGGT GGCTTATGGT GCTGTACGAG CGGGACTGCC GGCGGCGCTT CACCCCCGAG 
GACCACTGGC TGCGAAAGGA TCTCAAACCT AGCGTGCTCT TCCAAGAACT CGACAGGGAC 
AGAAAACGGG CACAGTTGAT CCTGCAGTAC ATCCCACATG TCATCCCTCA CAAAAACAGA 
GTTCTACTGT TTCGAACCAT GGTTACCAAG GAGAAGGAGA AACTGGGGCT GGTGGAAACC 
AGCTCTGCCT CCCCGCATGT CACTCACATC ACCATCCGCC GGTCCAGGAT GCTGGAGGAC 
GGCTACGAGC AGCTTAGGCA GCTCTCCCAG CACGCCATGA AGGGGGTCAT CCGTGTGAAG 
TTTGTCAATG ACCTCGGGGT GGACGAAGCA GGGATTGATC AAGACGGTGT TTTTAAGGAG 
TTCTTGGAAG AGATCATCAA GAGAGTTTTT GACCCAGCAC TCAATCTGTT CAAGACAACC 
AGTGGGGATG AGAGGCTGTA CCCCTCACCC ACATCCTACA TCCATGAGAA TTACCTGCAG 
CTCTTCGAGT TTGTGGGGAA GATGCTGGGG AAGGCTGTGT ATGAGGGAAT TGTGGTGGAC 
GTGCCATTTG CATCCTTCTT CCTGAGCCAA CTGCTTGGGC ACCACCACAG CGTCTTCTAT 
AGCTCGGTGG ATGAACTGCC TTCTCTGGAC TCCGAGTTCT ATAAAAACCT CACCTCCATC 
AAGCGCTATG ATGGGGACAT CACTGACCTG GGCCTGACGC TGTCTTACGA CGAGGACGTC 
ATGGGTCAGC TTGTTTGCCA TGAACTGATT CCTGGAGGGA AGACCATTCC TGTTACAAAT 
GAAAATAAAA TTAGCTACAT CCATCTGATG GCACATTTTC GAATGCACAC TCAAATAAAA 
AACCAAACAG CTGCCCTCAT TAGCGGATTC CGTTCCATTA TCAAACCCGA GTGGATCCGA 
ATGTTCTCAA CTCCTGAACT GCAGCGTCTC ATCTCTGGCG ACAATGCTGA GATTGATCTG 
GAAGATTTAA AGAAGCACAC AGTCTACTAC GGTGGTTTCC ATGGAAGTCA CAGAGTCATC 
ATCTGGCTCT GGGATATTCT GGCCTCCGAC TTCACACCGG ATGAGAGAGC TATGTTTCTG 
AAGTTCGTGA CCAGCTGCTC CAGACCCCCG CTCCTGGGAT TCGCCTACCT CAAGCCTCCA 
TTCTCCATCC GCTGCGTGGA GGTGTCGGAC GATCAGGACA CCGGGGACAC TCTGGGCAGC 
GTCCTCCGGG GCTTCTTCAC CATCCGCAAG CGGGAGCCAG GCGGCCGCCT GCCCACCTCC 
TCCACCTGCT TCAACCTGCT CAAGCTGCCC AACTACAGCA AGAAGAGCGT CCTCCGCGAG 
AAGCTGCGCT ACGCCATCAG CATGAACACG GGCTTTGAAC TCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_006719681.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719744.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu46963
Clone ID Related Accession (Same CDS sequence)	XM_006719681.3
Accession Version	XM_006719681.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719681.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGCAGGC TGTGCACGTC CAGGCTCGCT GCTTCATTTC CTCATTGGCC 
AAAGGAGAAG GAAAGCCTTC AGGGTTACGG AGCTCTGTGT GTCTCAAGCC TGGCCCACTG 
TGGGTGCTCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719744.1
CDS	828..3962
Translation	MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLESHDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCRKYVSQKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASPQNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQELDRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDITDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQQAVHVQARCFISSLAKGEGKPSGLRSSVCLKPGPLWVLK

Target ORF information:

RefSeq Version	XM_006719681.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719681.3

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGCAGGC TGTGCACGTC CAGGCTCGCT GCTTCATTTC CTCATTGGCC 
AAAGGAGAAG GAAAGCCTTC AGGGTTACGG AGCTCTGTGT GTCTCAAGCC TGGCCCACTG 
TGGGTGCTCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_006719682.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719745.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu46964
Clone ID Related Accession (Same CDS sequence)	XM_006719682.2
Accession Version	XM_006719682.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719682.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGCTGT GCACGTCCAG GCTCGCTGCT TCATTTCCTC ATTGGCCAAA 
GGAGAAGGAA AGCCTTCAGG GTTACGGAGC TCTGTGTGTC TCAAGCCTGG CCCACTGTGG 
GTGCTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719745.1
CDS	828..3959
Translation	MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLESHDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCRKYVSQKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASPQNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQELDRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDITDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQAVHVQARCFISSLAKGEGKPSGLRSSVCLKPGPLWVLK

Target ORF information:

RefSeq Version	XM_006719682.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719682.2

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGCTGT GCACGTCCAG GCTCGCTGCT TCATTTCCTC ATTGGCCAAA 
GGAGAAGGAA AGCCTTCAGG GTTACGGAGC TCTGTGTGTC TCAAGCCTGG CCCACTGTGG 
GTGCTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_011538961.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537263.1 Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu74142
Clone ID Related Accession (Same CDS sequence)	XM_011538961.1
Accession Version	XM_011538961.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin-protein ligase E3B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGAGCC ACACTTTAAG AAGGATGCAG GAGGCTGGGC GTGGTGGCTC 
ACGCCTGTAA TCCCAACACT TTCGGAGGCA GATGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537263.1
CDS	828..3926
Translation	MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTKRSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWYCCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLESHDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCRKYVSQKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASPQNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQELDRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDITDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQEPHFKKDAGGWAWWLTPVIPTLSEADG

Target ORF information:

RefSeq Version	XM_011538961.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin protein ligase E3B (UBE3B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538961.1

ORF Insert Sequence:

ATGTTCACCC TGTCTCAGAC CTCGAGAGCA TGGTTCATCG ATAGAGCCCG TCAGGCACGA 
GAAGAAAGGC TTGTGCAGAA GGAACGGGAG CGGGCAGCTG TTGTGATCCA GGCCCATGTC 
CGGAGTTTTC TCTGTCGGAG TCGACTGCAG AGAGATATCA GGAGAGAGAT TGATGACTTT 
TTTAAAGCAG ATGACCCTGA GTCCACTAAA AGAAGTGCAC TTTGTATTTT CAAGATTGCC 
AGGAAACTGC TGTTCCTATT CAGAATCAAA GAGGATAATG AGAGATTTGA GAAGTTGTGT 
CGCAGCATCC TGAGCAGCAT GGATGCTGAG AATGAGCCTA AGGTGTGGTA TGTGTCCCTG 
GCTTGTTCTA AGGACCTCAC CCTCCTTTGG ATTCAACAGA TCAAGAACAT TTTGTGGTAC 
TGCTGTGATT TTCTCAAGCA GCTCAAGCCT GAAATCCTGC AGGACTCCCG ACTCATCACC 
CTGTACCTCA CGATGCTTGT CACCTTCACA GACACTTCAA CGTGGAAAAT TCTTCGGGGA 
AAAGGTGAAA GTCTTCGACC AGCGATGAAC CACATTTGTG CAAATATAAT GGGACATCTC 
AACCAGCATG GATTTTATTC TGTGCTGCAG ATATTGTTAA CCCGTGGCCT GGCAAGACCC 
CGTCCTTGTC TATCCAAAGG CACTTTAACA GCAGCTTTTT CTCTAGCGTT ACGCCCTGTG 
ATTGCTGCAC AGTTCTCAGA CAATCTGATT CGGCCGTTCC TCATCCACAT CATGTCTGTG 
CCTGCTCTGG TGACTCATCT CAGCACAGTG ACCCCTGAGC GCCTCACTGT TTTAGAATCC 
CATGACATGC TTCGTAAATT CATCATATTT TTAAGAGACC AAGATCGATG CCGTGATGTA 
TGTGAAAGTT TAGAAGGATG CCATACGCTT TGTCTAATGG GCAACCTCCT ACACTTGGGC 
TCCCTCAGCC CCAGAGTGTT AGAGGAGGAG ACAGATGGGT TCGTGAGTTT GCTCACCCAG 
ACGCTGTGCT ACTGTCGGAA GTATGTGTCT CAGAAGAAGT CCAACCTGAC CCACTGGCAT 
CCTGTCCTTG GCTGGTTCTC CCAATCTGTG GATTATGGCC TTAACGAGTC AATGCACTTG 
ATCACCAAAC AGCTGCAGTT CTTGTGGGGG GTGCCTCTGA TCCGGATCTT CTTCTGTGAC 
ATCCTGAGCA AGAAGCTACT GGAGAGCCAG GAGCCAGCCC ACGCACAGCC AGCATCCCCT 
CAGAATGTGC TCCCAGTGAA GAGTCTCCTA AAGCGTGCTT TTCAAAAGTC GGCATCAGTC 
CGGAATATTC TCAGGCCTGT CGGGGGTAAA CGGGTCGACT CTGCAGAAGT CCAGAAGGTT 
TGCAACATCT GTGTCCTCTA CCAGACCTCG CTGACAACTC TCACACAGAT TCGGCTGCAG 
ATACTCACAG GTCTCACTTA CCTTGATGAC CTGCTTCCCA AACTGTGGGC ATTTATCTGT 
GAGCTCGGGC CCCACGGAGG GTTAAAGCTC TTCTTGGAAT GCCTGAACAA TGACACTGAA 
GAGTCCAAGC AACTCTTGGC CATGCTGATG CTGTTCTGTG ACTGTTCGCG GCACCTCATC 
ACAATCCTTG ATGACATTGA AGTTTATGAA GAACAGATTT CATTCAAACT GGAAGAGCTG 
GTCACTATCT CCTCTTTCCT GAATTCTTTT GTGTTTAAGA TGATCTGGGA TGGAATTGTA 
GAGAACGCCA AGGGTGAGAC CTTGGAGCTG TTCCAGTCTG TCCACGGGTG GCTTATGGTG 
CTGTACGAGC GGGACTGCCG GCGGCGCTTC ACCCCCGAGG ACCACTGGCT GCGAAAGGAT 
CTCAAACCTA GCGTGCTCTT CCAAGAACTC GACAGGGACA GAAAACGGGC ACAGTTGATC 
CTGCAGTACA TCCCACATGT CATCCCTCAC AAAAACAGAG TTCTACTGTT TCGAACCATG 
GTTACCAAGG AGAAGGAGAA ACTGGGGCTG GTGGAAACCA GCTCTGCCTC CCCGCATGTC 
ACTCACATCA CCATCCGCCG GTCCAGGATG CTGGAGGACG GCTACGAGCA GCTTAGGCAG 
CTCTCCCAGC ACGCCATGAA GGGGGTCATC CGTGTGAAGT TTGTCAATGA CCTCGGGGTG 
GACGAAGCAG GGATTGATCA AGACGGTGTT TTTAAGGAGT TCTTGGAAGA GATCATCAAG 
AGAGTTTTTG ACCCAGCACT CAATCTGTTC AAGACAACCA GTGGGGATGA GAGGCTGTAC 
CCCTCACCCA CATCCTACAT CCATGAGAAT TACCTGCAGC TCTTCGAGTT TGTGGGGAAG 
ATGCTGGGGA AGGCTGTGTA TGAGGGAATT GTGGTGGACG TGCCATTTGC ATCCTTCTTC 
CTGAGCCAAC TGCTTGGGCA CCACCACAGC GTCTTCTATA GCTCGGTGGA TGAACTGCCT 
TCTCTGGACT CCGAGTTCTA TAAAAACCTC ACCTCCATCA AGCGCTATGA TGGGGACATC 
ACTGACCTGG GCCTGACGCT GTCTTACGAC GAGGACGTCA TGGGTCAGCT TGTTTGCCAT 
GAACTGATTC CTGGAGGGAA GACCATTCCT GTTACAAATG AAAATAAAAT TAGCTACATC 
CATCTGATGG CACATTTTCG AATGCACACT CAAATAAAAA ACCAAACAGC TGCCCTCATT 
AGCGGATTCC GTTCCATTAT CAAACCCGAG TGGATCCGAA TGTTCTCAAC TCCTGAACTG 
CAGCGTCTCA TCTCTGGCGA CAATGCTGAG ATTGATCTGG AAGATTTAAA GAAGCACACA 
GTCTACTACG GTGGTTTCCA TGGAAGTCAC AGAGTCATCA TCTGGCTCTG GGATATTCTG 
GCCTCCGACT TCACACCGGA TGAGAGAGCT ATGTTTCTGA AGTTCGTGAC CAGCTGCTCC 
AGACCCCCGC TCCTGGGATT CGCCTACCTC AAGCCTCCAT TCTCCATCCG CTGCGTGGAG 
GTGTCGGACG ATCAGGAGCC ACACTTTAAG AAGGATGCAG GAGGCTGGGC GTGGTGGCTC 
ACGCCTGTAA TCCCAACACT TTCGGAGGCA GATGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	UBE3B
Entrez Gene ID	89910
Full Name	ubiquitin protein ligase E3B
Synonyms	BPIDS,KOS
General protein information	Preferred Names ubiquitin protein ligase E3B Names ubiquitin-protein ligase E3B HECT-type ubiquitin transferase E3B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.11
Summary	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2012].
Disorder MIM:	608047
Disorder Html:	Kaufman oculocerebrofacial syndrome, 244450 (3)

Xenopus tropicalis (tropical clawed frog)	ube3b	NP_001072875.1
Pan troglodytes (chimpanzee)	UBE3B	XP_001137003.2
Danio rerio (zebrafish)	ube3b	NP_001108154.1
	UBE3B	XP_002798818.1
Homo sapiens (human)	UBE3B	NP_569733.2
Canis lupus familiaris (dog)	UBE3B	XP_543437.2
Bos taurus (cattle)	UBE3B	NP_001193161.1
Drosophila melanogaster (fruit fly)	CG5087	NP_001261610.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005897	XP_315926.2
Caenorhabditis elegans (roundworm)	oxi-1	NP_499392.1
Mus musculus (house mouse)	Ube3b	NP_473434.2
Rattus norvegicus (Norway rat)	Ube3b	NP_001137366.1
Gallus gallus (chicken)	LOC776286	XP_003642246.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

UBE3B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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