DGKD cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKD
Entrez Gene ID	8527
Full Name	diacylglycerol kinase delta
Synonyms	DGKdelta,dgkd-2
General protein information	Preferred Names diacylglycerol kinase delta Names diacylglycerol kinase delta DAG kinase delta DGK-delta diacylglycerol kinase, delta 130kDa diglyceride kinase delta
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q37.1
Summary	This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
Disorder MIM:	601826

mRNA and Protein(s)

mRNA	Protein	Name
XM_005246110.3	XP_005246167.1	diacylglycerol kinase delta isoform X11
NM_152879.2	NP_690618.2	diacylglycerol kinase delta isoform 2
XM_011512028.3	XP_011510330.1	diacylglycerol kinase delta isoform X1
XM_011512029.3	XP_011510331.1	diacylglycerol kinase delta isoform X2
XM_011512032.3	XP_011510334.1	diacylglycerol kinase delta isoform X5
NM_001377259.1	NP_001364188.1	diacylglycerol kinase delta isoform 3
XM_011512032.2	XP_011510334.1	diacylglycerol kinase delta isoform X5
XM_011512028.2	XP_011510330.1	diacylglycerol kinase delta isoform X1
XM_017005138.2	XP_016860627.1	diacylglycerol kinase delta isoform X3
XM_011512035.1	XP_011510337.1	diacylglycerol kinase delta isoform X9
XM_024453189.1	XP_024308957.1	diacylglycerol kinase delta isoform X11
XM_011512031.2	XP_011510333.1	diacylglycerol kinase delta isoform X5
XM_011512030.2	XP_011510332.1	diacylglycerol kinase delta isoform X4
XM_017005139.1	XP_016860628.1	diacylglycerol kinase delta isoform X6
NM_152879.3	NP_690618.2	diacylglycerol kinase delta isoform 2
XM_005246107.3	XP_005246164.1	diacylglycerol kinase delta isoform X8
XM_017005140.1	XP_016860629.1	diacylglycerol kinase delta isoform X9
NM_003648.3	NP_003639.2	diacylglycerol kinase delta isoform 1
XM_011512040.2	XP_011510342.1	diacylglycerol kinase delta isoform X9
XM_011512037.2	XP_011510339.1	diacylglycerol kinase delta isoform X9
XM_011512029.2	XP_011510331.1	diacylglycerol kinase delta isoform X2
XM_017005138.1	XP_016860627.1	diacylglycerol kinase delta isoform X3
XM_011512033.2	XP_011510335.1	diacylglycerol kinase delta isoform X7
NM_003648.2	NP_003639.2	diacylglycerol kinase delta isoform 1
XM_005246108.1	XP_005246165.1	diacylglycerol kinase delta isoform X9
XM_011512031.3	XP_011510333.1	diacylglycerol kinase delta isoform X5
XM_011512033.3	XP_011510335.1	diacylglycerol kinase delta isoform X7
XM_011512039.2	XP_011510341.1	diacylglycerol kinase delta isoform X10

Pathways from BioSystems

Homologies

Homo sapiens (human)	DGKD	NP_690618.2
Pan troglodytes (chimpanzee)	DGKD	XP_003309576.1
Gallus gallus (chicken)	DGKD	XP_004937002.1
Xenopus tropicalis (tropical clawed frog)	dgkd	XP_002936660.1
Rattus norvegicus (Norway rat)	Dgkd	XP_006245566.1
Mus musculus (house mouse)	Dgkd	NP_808314.2
	DGKD	XP_001114920.1
Canis lupus familiaris (dog)	DGKD	XP_005635924.1
Bos taurus (cattle)	DGKD	NP_001030359.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression, Purification, and Properties of a Human Arachidonoyl-Specific Isoform of Diacylglycerol Kinase.
Jennings W, Doshi S, Hota PK, Prodeus A, Black S, Epand RM
Biochemistry56(9)1337-1347(2017 03)

A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome.
Blandin G, Marchand S, Charton K, Dani?le N, Gicquel E, Boucheteil JB, Bentaib A, Barrault L, Stockholm D, Bartoli M, Richard I
Skeletal muscle3(1)3(2013 Feb)

Diacylglycerol kinase ? modulates Akt phosphorylation through pleckstrin homology domain leucine-rich repeat protein phosphatase 2 (PHLPP2).
Crotty TM, Nakano T, Stafforini DM, Topham MK
The Journal of biological chemistry288(3)1439-47(2013 Jan)

Meta-analysis of genome-wide association studies identifies six new Loci for serum calcium concentrations.
O'Seaghdha CM, Wu H, Yang Q, Kapur K, Guessous I, Zuber AM, K?ttgen A, Stoudmann C, Teumer A, Kutalik Z, Mangino M, Dehghan A, Zhang W, Eiriksdottir G, Li G, Tanaka T, Portas L, Lopez LM, Hayward C, Lohman K, Matsuda K, Padmanabhan S, Firsov D, Sorice R, Ulivi S, Brockhaus AC, Kleber ME, Mahajan A, Ernst FD, Gudnason V, Launer LJ, Mace A, Boerwinckle E, Arking DE, Tanikawa C, Nakamura Y, Brown MJ, Gaspoz JM, Theler JM, Siscovick DS, Psaty BM, Bergmann S, Vollenweider P, Vitart V, Wright AF, Zemunik T, Boban M, Kolcic I, Navarro P, Brown EM, Estrada K, Ding J, Harris TB, Bandinelli S, Hernandez D, Singleton AB, Girotto G, Ruggiero D, d'Adamo AP, Robino A, Meitinger T, Meisinger C, Davies G, Starr JM, Chambers JC, Boehm BO, Winkelmann BR, Huang J, Murgia F, Wild SH, Campbell H, Morris AP, Franco OH, Hofman A, Uitterlinden AG, Rivadeneira F, V?lker U, Hannemann A, Biffar R, Hoffmann W, Shin SY, Lescuyer P, Henry H, Schurmann C, , , Munroe PB, Gasparini P, Pirastu N, Ciullo M, Gieger C, M?rz W, Lind L, Spector TD, Smith AV, Rudan I, Wilson JF, Polasek O, Deary IJ, Pirastu M, Ferrucci L, Liu Y, Kestenbaum B, Kooner JS, Witteman JC, Nauck M, Kao WH, Wallaschofski H, Bonny O, Fox CS, Bochud M
PLoS genetics9(9)e1003796(2013)

A genome-wide scan of Ashkenazi Jewish Crohn's disease suggests novel susceptibility loci.
Kenny EE, Pe'er I, Karban A, Ozelius L, Mitchell AA, Ng SM, Erazo M, Ostrer H, Abraham C, Abreu MT, Atzmon G, Barzilai N, Brant SR, Bressman S, Burns ER, Chowers Y, Clark LN, Darvasi A, Doheny D, Duerr RH, Eliakim R, Giladi N, Gregersen PK, Hakonarson H, Jones MR, Marder K, McGovern DP, Mulle J, Orr-Urtreger A, Proctor DD, Pulver A, Rotter JI, Silverberg MS, Ullman T, Warren ST, Waterman M, Zhang W, Bergman A, Mayer L, Katz S, Desnick RJ, Cho JH, Peter I
PLoS genetics8(3)e1002559(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

purification and characterization of DGKepsilon's enzymatic and conformational properties
Title: Expression, Purification, and Properties of a Human Arachidonoyl-Specific Isoform of Diacylglycerol Kinase.

Diacylglycerol signaling limits Akt activation through diacylglycerol kinase delta and PHLPP2.
Title: Diacylglycerol kinase ? modulates Akt phosphorylation through pleckstrin homology domain leucine-rich repeat protein phosphatase 2 (PHLPP2).

This study demonistrated that the lack of association of DGKD SNPs with PD in the Han Chinese population.
Title: Analysis of the genotype of diacylglycerol kinase delta single-nucleotide polymorphisms in Parkinson disease in the Han Chinese population.

Zinc site mutations impair DGKdelta localization to cytoplasmic puncta and enhance localization the plasma membrane.
Title: Zinc binding drives sheet formation by the SAM domain of diacylglycerol kinase ?.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following DGKD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu36705	XM_005246110.3 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu61930	XM_011512039.2 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu104988	XM_024453189.1 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu36703	NM_001377259.1 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu90009	XM_017005139.1 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu14020	NM_003648.3 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36702	XM_005246107.3 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu36703	XM_011512037.2 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu36703	XM_011512035.1 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu36703	XM_011512040.2 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu36703	XM_017005140.1 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu36703	XM_005246108.1 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu61923	XM_011512028.3 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61924	XM_011512029.3 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61925	XM_011512030.2 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61926	XM_011512032.3 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu61926	XM_011512031.3 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu61927	XM_011512033.3 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu61928	XM_017005138.2 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu17931	NM_152879.3 Latest version!	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$860.30 $1229.00
View Old Accession Versions >>
OHu61926	XM_011512032.2	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu61923	XM_011512028.2	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu17931	NM_152879.2	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$860.30 $1229.00
OHu61924	XM_011512029.2	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61928	XM_017005138.1	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu14020	NM_003648.2	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61926	XM_011512031.2	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu61927	XM_011512033.2	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKD ( XM_005246110.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246167.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu36705
Clone ID Related Accession (Same CDS sequence)	XM_005246110.3
Accession Version	XM_005246110.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005246110.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGA AGTGCTCTGT CCTGAAAGAG AAGCTGGATT CCCTTCTCAA GACCTTGGAC 
GATGAGTCCC AGGCCTCGTC CTCTCTGCCC AACCCGCCCC CCACCATTGC CGAGGAGGCT 
GAAGATGGAG ATGGGTCGGG CAGCATCTGC GGTTCCACCG GAGACCGCTT GGTGGCATCA 
GCTTGCCCGG CCCGGCCGCA GATATTCCGG CCTCGAGAAC AGCTCATGCT GAGAGCCAAC 
AGCCTGAAGA AAGCAATTCG TCAGATCATA GAACACACAG AAAAAGCTGT CGATGAGCAG 
AATGCCCAGA CCCAGGAGCA GGAGGGCTTC GTCCTGGGCC TCTCTGAGTC AGAGGAGAAG 
ATGGACCACA GAGTGTGCCC ACCACTGTCC CACAGCGAGA GCTTCGGGGT CCCCAAGGGG 
AGGAGCCAGC GCAAAGTGTC GAAATCTCCG TGTGAAAAGC TGATCAGCAA AGGGAGTCTG 
TCCCTAGGCA GTTCTGCTTC CCTTCCGCCC CAGCCGGGAA GCCGGGACGG CCTGCCTGCG 
CTCAACACCA AGATCCTGTA CCCAAATGTC CGGGCTGGAA TGTCTGGTTC CTTACCCGGT 
GGCTCAGTCA TCAGTCGCCT GTTAATTAAT GCTGATCCCT TCAACTCTGA ACCAGAAACC 
CTAGAGTATT ACACGGAGAA ATGTGTCATG AACAACTATT TTGGCATTGG CCTGGATGCG 
AAGATATCCC TGGACTTTAA CAACAAGCGC GATGAGCACC CAGAGAAGTG CAGGAGCCGA 
ACCAAGAACA TGATGTGGTA TGGAGTTCTT GGAACCAAAG AGTTGCTGCA CAGAACCTAC 
AAGAACCTGG AGCAAAAGGT CTTGCTGGAG TGTGACGGGC GACCCATCCC ACTCCCCAGT 
CTTCAGGGAA TTGCTGTCCT TAACATTCCC AGCTATGCCG GAGGAACCAA CTTCTGGGGG 
GGTACCAAGG AAGATGATAC TTTCGCAGCT CCATCATTCG ATGACAAGAT TCTGGAGGTG 
GTCGCCGTGT TCGGCAGCAT GCAGATGGCC GTCTCTCGAG TCATCAGGCT ACAGCATCAT 
CGGATCGCCC AGTGTCGCAC GGTGAAGATC TCCATCCTTG GGGATGAGGG CGTGCCTGTG 
CAGGTGGACG GAGAGGCCTG GGTCCAGCCG CCAGGGTACA TTCGGATTGT CCACAAGAAC 
CGGGCACAGA CACTGACCAG AGACAGGGCA TTTGAGAGCA CCCTGAAGTC CTGGGAAGAC 
AAGCAGAAGT GCGAGCTGCC CCGCCCTCCA TCCTGTTCCC TGCACCCGGA GATGCTGTCC 
GAGGAGGAGG CCACCCAGAT GGACCAGTTT GGGCAGGCAG CAGGGGTCCT CATTCACAGT 
ATCCGAGAAA TAGCTCAGTC TCACCGGGAC ATGGAGCAGG AACTGGCCCA CGCCGTCAAT 
GCCAGCTCCA AGTCCATGGA CCGTGTGTAT GGCAAGCCCA GAACCACAGA GGGGCTCAAC 
TGCAGCTTCG TCCTGGAAAT GGTGAATAAC TTCAGAGCTC TGCGCAGTGA GACGGAGCTG 
CTGCTGTCTG GGAAGATGGC CCTGCAGCTG GATCCGCCTC AGAAGGAGCA GCTGGGGAGT 
GCTCTTGCCG AGATGGACCG ACAGCTCAGG AGGCTGGCAG ACACCCCGTG GCTCTGCCAG 
TCCGCAGAGC CCGGCGACGA AGAGAGTGTG ATGCTGGATC TTGCCAAGCG CAGTCGCAGT 
GGTAAATTCC GCCTCGTGAC CAAGTTTAAA AAGGAGAAAA ACAACAAGAA CAAAGAAGCT 
CACAGTAGCC TGGGAGCCCC GGTTCACCTC TGGGGGACAG AGGAGGTTGC TGCCTGGCTG 
GAGCACCTCA GTCTCTGTGA GTATAAGGAC ATCTTCACAC GGCACGACAT CCGGGGCTCT 
GAGCTCCTGC ACCTGGAGCG GAGGGACCTC AAGGACCTGG GCGTGACCAA GGTGGGCCAC 
ATGAAGAGGA TCCTGTGTGG CATCAAGGAG CTGAGCCGCA GCGCCCCCGC CGTCGAGGCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246167.1
CDS	1287..3329
Translation	MAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_005246110.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246110.3

ORF Insert Sequence:

ATGGCCAAGA AGTGCTCTGT CCTGAAAGAG AAGCTGGATT CCCTTCTCAA GACCTTGGAC 
GATGAGTCCC AGGCCTCGTC CTCTCTGCCC AACCCGCCCC CCACCATTGC CGAGGAGGCT 
GAAGATGGAG ATGGGTCGGG CAGCATCTGC GGTTCCACCG GAGACCGCTT GGTGGCATCA 
GCTTGCCCGG CCCGGCCGCA GATATTCCGG CCTCGAGAAC AGCTCATGCT GAGAGCCAAC 
AGCCTGAAGA AAGCAATTCG TCAGATCATA GAACACACAG AAAAAGCTGT CGATGAGCAG 
AATGCCCAGA CCCAGGAGCA GGAGGGCTTC GTCCTGGGCC TCTCTGAGTC AGAGGAGAAG 
ATGGACCACA GAGTGTGCCC ACCACTGTCC CACAGCGAGA GCTTCGGGGT CCCCAAGGGG 
AGGAGCCAGC GCAAAGTGTC GAAATCTCCG TGTGAAAAGC TGATCAGCAA AGGGAGTCTG 
TCCCTAGGCA GTTCTGCTTC CCTTCCGCCC CAGCCGGGAA GCCGGGACGG CCTGCCTGCG 
CTCAACACCA AGATCCTGTA CCCAAATGTC CGGGCTGGAA TGTCTGGTTC CTTACCCGGT 
GGCTCAGTCA TCAGTCGCCT GTTAATTAAT GCTGATCCCT TCAACTCTGA ACCAGAAACC 
CTAGAGTATT ACACGGAGAA ATGTGTCATG AACAACTATT TTGGCATTGG CCTGGATGCG 
AAGATATCCC TGGACTTTAA CAACAAGCGC GATGAGCACC CAGAGAAGTG CAGGAGCCGA 
ACCAAGAACA TGATGTGGTA TGGAGTTCTT GGAACCAAAG AGTTGCTGCA CAGAACCTAC 
AAGAACCTGG AGCAAAAGGT CTTGCTGGAG TGTGACGGGC GACCCATCCC ACTCCCCAGT 
CTTCAGGGAA TTGCTGTCCT TAACATTCCC AGCTATGCCG GAGGAACCAA CTTCTGGGGG 
GGTACCAAGG AAGATGATAC TTTCGCAGCT CCATCATTCG ATGACAAGAT TCTGGAGGTG 
GTCGCCGTGT TCGGCAGCAT GCAGATGGCC GTCTCTCGAG TCATCAGGCT ACAGCATCAT 
CGGATCGCCC AGTGTCGCAC GGTGAAGATC TCCATCCTTG GGGATGAGGG CGTGCCTGTG 
CAGGTGGACG GAGAGGCCTG GGTCCAGCCG CCAGGGTACA TTCGGATTGT CCACAAGAAC 
CGGGCACAGA CACTGACCAG AGACAGGGCA TTTGAGAGCA CCCTGAAGTC CTGGGAAGAC 
AAGCAGAAGT GCGAGCTGCC CCGCCCTCCA TCCTGTTCCC TGCACCCGGA GATGCTGTCC 
GAGGAGGAGG CCACCCAGAT GGACCAGTTT GGGCAGGCAG CAGGGGTCCT CATTCACAGT 
ATCCGAGAAA TAGCTCAGTC TCACCGGGAC ATGGAGCAGG AACTGGCCCA CGCCGTCAAT 
GCCAGCTCCA AGTCCATGGA CCGTGTGTAT GGCAAGCCCA GAACCACAGA GGGGCTCAAC 
TGCAGCTTCG TCCTGGAAAT GGTGAATAAC TTCAGAGCTC TGCGCAGTGA GACGGAGCTG 
CTGCTGTCTG GGAAGATGGC CCTGCAGCTG GATCCGCCTC AGAAGGAGCA GCTGGGGAGT 
GCTCTTGCCG AGATGGACCG ACAGCTCAGG AGGCTGGCAG ACACCCCGTG GCTCTGCCAG 
TCCGCAGAGC CCGGCGACGA AGAGAGTGTG ATGCTGGATC TTGCCAAGCG CAGTCGCAGT 
GGTAAATTCC GCCTCGTGAC CAAGTTTAAA AAGGAGAAAA ACAACAAGAA CAAAGAAGCT 
CACAGTAGCC TGGGAGCCCC GGTTCACCTC TGGGGGACAG AGGAGGTTGC TGCCTGGCTG 
GAGCACCTCA GTCTCTGTGA GTATAAGGAC ATCTTCACAC GGCACGACAT CCGGGGCTCT 
GAGCTCCTGC ACCTGGAGCG GAGGGACCTC AAGGACCTGG GCGTGACCAA GGTGGGCCAC 
ATGAAGAGGA TCCTGTGTGG CATCAAGGAG CTGAGCCGCA GCGCCCCCGC CGTCGAGGCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512039.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510341.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu61930
Clone ID Related Accession (Same CDS sequence)	XM_011512039.2
Accession Version	XM_011512039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512039.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTTC AGACTCCAGG GACAGCTGTC ACCCACCAAA AACTGGGGTC ACAAGGGGTC 
CATCTGGCAG TGGCTGGAGC CTTGCAAGGG CACAGCTGCT GCTGCGGATG TACTGTTCCC 
CTCCTGCCTG GGAACCCTGG CCTTGCTGGG GTCACCTCCT CCACAACTTG GACTGGAGCA 
GAAGGACAAA GAATGGATCT GAGGACAAGC TGGCCCAGGA CCAGAACTAC CATCATCAAA 
GAGGGGATGC TGACCAAACA GAACAATTCA TTCCAGCGAT CAAAAAGGAG ATACTTTAAG 
CTTCGAGGGC GAACGCTTTA CTATGCCAAA ACGGCAAAGT CAATCATATT TGATGAGGTG 
GATCTGACAG ATGCCAGCGT AGCTGAATCC AGTACCAAAA ACGTCAACAA CAGTTTTACG 
GTCATAACTC CATGCAGGAA GCTCATCTTG TGTGCTGATA ACAGAAAAGA AATGGAAGAT 
TGGATTGCAG CATTAAAGAC TGTGCAGAAC AGGGAGCACT TTGAGCCCAC CCAGTACAGC 
ATGGACCACT TCTCAGGGAT GCACAATTGG TACGCCTGTT CCCACGCGAG GCCGACCTAC 
TGCAATGTGT GCCGTGAGGC TCTGTCTGGG GTCACGTCGC ACGGGCTGTC CTGCGAGGTG 
TGCAAATTTA AGGCCCACAA GCGCTGTGCT GTGCGTGCAA CCAATAACTG CAAGTGGACC 
ACACTGGCCT CGATCGGGAA GGACATCATT GAAGATGCAG ATGGGATTGC AATGCCCCAC 
CAGTGGTTGG AAGGAAACCT ACCTGTGAGC GCCAAGTGCA CTGTGTGCGA CAAGACCTGT 
GGCAGTGTGC TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCGTGTAAAG AATCCTTGCT GACCAAGTGC CCACTTGGCC TGTGCAAAGT GTCAGTCATC 
CCACCCACGG CTCTCAACAG CATCGACTCC GATGGGTTCT GGAAGGCCAG CTGTCCTCCT 
TCTTGCACAA GCCCACTGTT GGTCTTCGTC AATTCAAAAA GTGGGGACAA CCAGGGTGTG 
AAGTTCCTCA GAAGATTCAA ACAGCTACTA AACCCCGCCC AGGTCTTCGA CCTCATGAAC 
GGAGGCCCAC ACCTCGGCTT ACGGTTATTC CAGAAGTTTG ACACATTCCG GATTCTGGTT 
TGTGGCGGGG ATGGAAGTGT TGGCTGGGTC CTCTCCGAAA TCGACAGCCT CAACCTTCAT 
AAACAGTGTC AGCTGGGAGT GCTGCCGCTC GGCACAGGGA ACGACTTGGC CCGAGTACTG 
GGCTGGGGCT CAGCCTGCGA TGACGACACC CAGCTCCCCC AGATCTTGGA GAAGTTGGAG 
AGAGCCAGCA CCAAGATGCT GGACAGGTGG AGCGTCATGG CATACGAGGC CAAGCTCCCC 
CGGCAGGCCT CCTCCTCTAC CGTCACCGAA GACTTCAGCG AGGATTCCGA GGTACAGCAG 
ATTCTCTTCT ATGAAGACTC GGTTGCAGCC CACCTTTCTA AAATCCTCAC CTCGGACCAG 
CACTCGGTGG TCATCTCCTC GGCCAAAGTG CTCTGTGAGA CGGTGAAGGA CTTCGTGGCA 
CGGGTGGGGA AGGCCTATGA GAAGACGACC GAGAGCTCGG AGGAGTCAGA GGTCATGGCC 
AAGAAGTGCT CTGTCCTGAA AGAGAAGCTG GATTCCCTTC TCAAGACCTT GGACGATGAG 
TCCCAGGCCT CGTCCTCTCT GCCCAACCCG CCCCCCACCA TTGCCGAGGA GGCTGAAGAT 
GGAGATGGGT CGGGCAGCAT CTGCGGTTCC ACCGGAGACC GCTTGGTGGC ATCAGCTTGC 
CCGGCCCGGC CGCAGATATT CCGGCCTCGA GAACAGCTCA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGTCAGAT CATAGAACAC ACAGAAAAAG CTGTCGATGA GCAGAATGCC 
CAGACCCAGG AGCAGGAGGG CTTCGTCCTG GGCCTCTCTG AGTCAGAGGA GAAGATGGAC 
CACAGAGTGT GCCCACCACT GTCCCACAGC GAGAGCTTCG GGGTCCCCAA GGGGAGGAGC 
CAGCGCAAAG TGTCGAAATC TCCGTGTGAA AAGCTGATCA GCAAAGGGAG TCTGTCCCTA 
GGCAGTTCTG CTTCCCTTCC GCCCCAGCCG GGAAGCCGGG ACGGCCTGCC TGCGCTCAAC 
ACCAAGATCC TGTACCCAAA TGTCCGGGCT GGAATGTCTG GTTCCTTACC CGGTGGCTCA 
GTCATCAGTC GCCTGTTAAT TAATGCTGAT CCCTTCAACT CTGAACCAGA AACCCTAGAG 
TATTACACGG AGAAATGTGT CATGAACAAC TATTTTGGCA TTGGCCTGGA TGCGAAGATA 
TCCCTGGACT TTAACAACAA GCGCGATGAG CACCCAGAGA AGTGCAGGAG CCGAACCAAG 
AACATGATGT GGTATGGAGT TCTTGGAACC AAAGAGTTGC TGCACAGAAC CTACAAGAAC 
CTGGAGCAAA AGGTCTTGCT GGAGTGTGAC GGGCGACCCA TCCCACTCCC CAGTCTTCAG 
GGAATTGCTG TCCTTAACAT TCCCAGCTAT GCCGGAGGAA CCAACTTCTG GGGGGGTACC 
AAGGAAGATG ATACTTTCGC AGCTCCATCA TTCGATGACA AGATTCTGGA GGTGGTCGCC 
GTGTTCGGCA GCATGCAGAT GGCCGTCTCT CGAGTCATCA GGCTACAGCA TCATCGGATC 
GCCCAGTGTC GCACGGTGAA GATCTCCATC CTTGGGGATG AGGGCGTGCC TGTGCAGGTG 
GACGGAGAGG CCTGGGTCCA GCCGCCAGGG TACATTCGGA TTGTCCACAA GAACCGGGCA 
CAGACACTGA CCAGAGACAG GTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510341.1
CDS	1402..4308
Translation	MAFQTPGTAVTHQKLGSQGVHLAVAGALQGHSCCCGCTVPLLPGNPGLAGVTSSTTWTGAEGQRMDLRTSWPRTRTTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRL

Target ORF information:

RefSeq Version	XM_011512039.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512039.2

ORF Insert Sequence:

ATGGCATTTC AGACTCCAGG GACAGCTGTC ACCCACCAAA AACTGGGGTC ACAAGGGGTC 
CATCTGGCAG TGGCTGGAGC CTTGCAAGGG CACAGCTGCT GCTGCGGATG TACTGTTCCC 
CTCCTGCCTG GGAACCCTGG CCTTGCTGGG GTCACCTCCT CCACAACTTG GACTGGAGCA 
GAAGGACAAA GAATGGATCT GAGGACAAGC TGGCCCAGGA CCAGAACTAC CATCATCAAA 
GAGGGGATGC TGACCAAACA GAACAATTCA TTCCAGCGAT CAAAAAGGAG ATACTTTAAG 
CTTCGAGGGC GAACGCTTTA CTATGCCAAA ACGGCAAAGT CAATCATATT TGATGAGGTG 
GATCTGACAG ATGCCAGCGT AGCTGAATCC AGTACCAAAA ACGTCAACAA CAGTTTTACG 
GTCATAACTC CATGCAGGAA GCTCATCTTG TGTGCTGATA ACAGAAAAGA AATGGAAGAT 
TGGATTGCAG CATTAAAGAC TGTGCAGAAC AGGGAGCACT TTGAGCCCAC CCAGTACAGC 
ATGGACCACT TCTCAGGGAT GCACAATTGG TACGCCTGTT CCCACGCGAG GCCGACCTAC 
TGCAATGTGT GCCGTGAGGC TCTGTCTGGG GTCACGTCGC ACGGGCTGTC CTGCGAGGTG 
TGCAAATTTA AGGCCCACAA GCGCTGTGCT GTGCGTGCAA CCAATAACTG CAAGTGGACC 
ACACTGGCCT CGATCGGGAA GGACATCATT GAAGATGCAG ATGGGATTGC AATGCCCCAC 
CAGTGGTTGG AAGGAAACCT ACCTGTGAGC GCCAAGTGCA CTGTGTGCGA CAAGACCTGT 
GGCAGTGTGC TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCGTGTAAAG AATCCTTGCT GACCAAGTGC CCACTTGGCC TGTGCAAAGT GTCAGTCATC 
CCACCCACGG CTCTCAACAG CATCGACTCC GATGGGTTCT GGAAGGCCAG CTGTCCTCCT 
TCTTGCACAA GCCCACTGTT GGTCTTCGTC AATTCAAAAA GTGGGGACAA CCAGGGTGTG 
AAGTTCCTCA GAAGATTCAA ACAGCTACTA AACCCCGCCC AGGTCTTCGA CCTCATGAAC 
GGAGGCCCAC ACCTCGGCTT ACGGTTATTC CAGAAGTTTG ACACATTCCG GATTCTGGTT 
TGTGGCGGGG ATGGAAGTGT TGGCTGGGTC CTCTCCGAAA TCGACAGCCT CAACCTTCAT 
AAACAGTGTC AGCTGGGAGT GCTGCCGCTC GGCACAGGGA ACGACTTGGC CCGAGTACTG 
GGCTGGGGCT CAGCCTGCGA TGACGACACC CAGCTCCCCC AGATCTTGGA GAAGTTGGAG 
AGAGCCAGCA CCAAGATGCT GGACAGGTGG AGCGTCATGG CATACGAGGC CAAGCTCCCC 
CGGCAGGCCT CCTCCTCTAC CGTCACCGAA GACTTCAGCG AGGATTCCGA GGTACAGCAG 
ATTCTCTTCT ATGAAGACTC GGTTGCAGCC CACCTTTCTA AAATCCTCAC CTCGGACCAG 
CACTCGGTGG TCATCTCCTC GGCCAAAGTG CTCTGTGAGA CGGTGAAGGA CTTCGTGGCA 
CGGGTGGGGA AGGCCTATGA GAAGACGACC GAGAGCTCGG AGGAGTCAGA GGTCATGGCC 
AAGAAGTGCT CTGTCCTGAA AGAGAAGCTG GATTCCCTTC TCAAGACCTT GGACGATGAG 
TCCCAGGCCT CGTCCTCTCT GCCCAACCCG CCCCCCACCA TTGCCGAGGA GGCTGAAGAT 
GGAGATGGGT CGGGCAGCAT CTGCGGTTCC ACCGGAGACC GCTTGGTGGC ATCAGCTTGC 
CCGGCCCGGC CGCAGATATT CCGGCCTCGA GAACAGCTCA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGTCAGAT CATAGAACAC ACAGAAAAAG CTGTCGATGA GCAGAATGCC 
CAGACCCAGG AGCAGGAGGG CTTCGTCCTG GGCCTCTCTG AGTCAGAGGA GAAGATGGAC 
CACAGAGTGT GCCCACCACT GTCCCACAGC GAGAGCTTCG GGGTCCCCAA GGGGAGGAGC 
CAGCGCAAAG TGTCGAAATC TCCGTGTGAA AAGCTGATCA GCAAAGGGAG TCTGTCCCTA 
GGCAGTTCTG CTTCCCTTCC GCCCCAGCCG GGAAGCCGGG ACGGCCTGCC TGCGCTCAAC 
ACCAAGATCC TGTACCCAAA TGTCCGGGCT GGAATGTCTG GTTCCTTACC CGGTGGCTCA 
GTCATCAGTC GCCTGTTAAT TAATGCTGAT CCCTTCAACT CTGAACCAGA AACCCTAGAG 
TATTACACGG AGAAATGTGT CATGAACAAC TATTTTGGCA TTGGCCTGGA TGCGAAGATA 
TCCCTGGACT TTAACAACAA GCGCGATGAG CACCCAGAGA AGTGCAGGAG CCGAACCAAG 
AACATGATGT GGTATGGAGT TCTTGGAACC AAAGAGTTGC TGCACAGAAC CTACAAGAAC 
CTGGAGCAAA AGGTCTTGCT GGAGTGTGAC GGGCGACCCA TCCCACTCCC CAGTCTTCAG 
GGAATTGCTG TCCTTAACAT TCCCAGCTAT GCCGGAGGAA CCAACTTCTG GGGGGGTACC 
AAGGAAGATG ATACTTTCGC AGCTCCATCA TTCGATGACA AGATTCTGGA GGTGGTCGCC 
GTGTTCGGCA GCATGCAGAT GGCCGTCTCT CGAGTCATCA GGCTACAGCA TCATCGGATC 
GCCCAGTGTC GCACGGTGAA GATCTCCATC CTTGGGGATG AGGGCGTGCC TGTGCAGGTG 
GACGGAGAGG CCTGGGTCCA GCCGCCAGGG TACATTCGGA TTGTCCACAA GAACCGGGCA 
CAGACACTGA CCAGAGACAG GTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_024453189.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308957.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X14, mRNA.

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CloneID	OHu104988
Clone ID Related Accession (Same CDS sequence)	XM_024453189.1
Accession Version	XM_024453189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGACA GGTGGAGCGT CATGGCATAC GAGGCCAAGC TCCCCCGGCA GGCCTCCTCC 
TCTACCGTCA CCGAAGACTT CAGCGAGGAT TCCGAGGTAC AGCAGATTCT CTTCTATGAA 
GACTCGGTTG CAGCCCACCT TTCTAAAATC CTCACCTCGG ACCAGCACTC GGTGGTCATC 
TCCTCGGCCA AAGTGCTCTG TGAGACGGTG AAGGACTTCG TGGCACGGGT GGGGAAGGCC 
TATGAGAAGA CGACCGAGAG CTCGGAGGAG TCAGAGGTCA TGGCCAAGAA GTGCTCTGTC 
CTGAAAGAGA AGCTGGATTC CCTTCTCAAG ACCTTGGACG ATGAGTCCCA GGCCTCGTCC 
TCTCTGCCCA ACCCGCCCCC CACCATTGCC GAGGAGGCTG AAGATGGAGA TGGGTCGGGC 
AGCATCTGCG GTTCCACCGG AGACCGCTTG GTGGCATCAG CTTGCCCGGC CCGGCCGCAG 
ATATTCCGGC CTCGAGAACA GCTCATGCTG AGAGCCAACA GCCTGAAGAA AGCAATTCGT 
CAGATCATAG AACACACAGA AAAAGCTGTC GATGAGCAGA ATGCCCAGAC CCAGGAGCAG 
GAGGGCTTCG TCCTGGGCCT CTCTGAGTCA GAGGAGAAGA TGGACCACAG AGTGTGCCCA 
CCACTGTCCC ACAGCGAGAG CTTCGGGGTC CCCAAGGGGA GGAGCCAGCG CAAAGTGTCG 
AAATCTCCGT GTGAAAAGCT GATCAGCAAA GGGAGTCTGT CCCTAGGCAG TTCTGCTTCC 
CTTCCGCCCC AGCCGGGAAG CCGGGACGGC CTGCCTGCGC TCAACACCAA GATCCTGTAC 
CCAAATGTCC GGGCTGGAAT GTCTGGTTCC TTACCCGGTG GCTCAGTCAT CAGTCGCCTG 
TTAATTAATG CTGATCCCTT CAACTCTGAA CCAGAAACCC TAGAGTATTA CACGGAGAAA 
TGTGTCATGA ACAACTATTT TGGCATTGGC CTGGATGCGA AGATATCCCT GGACTTTAAC 
AACAAGCGCG ATGAGCACCC AGAGAAGTGC AGGAGCCGAA CCAAGAACAT GATGTGGTAT 
GGAGTTCTTG GAACCAAAGA GTTGCTGCAC AGAACCTACA AGAACCTGGA GCAAAAGGTC 
TTGCTGGAGT GTGACGGGCG ACCCATCCCA CTCCCCAGTC TTCAGGGAAT TGCTGTCCTT 
AACATTCCCA GCTATGCCGG AGGAACCAAC TTCTGGGGGG GTACCAAGGA AGATGATACT 
TTCGCAGCTC CATCATTCGA TGACAAGATT CTGGAGGTGG TCGCCGTGTT CGGCAGCATG 
CAGATGGCCG TCTCTCGAGT CATCAGGCTA CAGCATCATC GGATCGCCCA GTGTCGCACG 
GTGAAGATCT CCATCCTTGG GGATGAGGGC GTGCCTGTGC AGGTGGACGG AGAGGCCTGG 
GTCCAGCCGC CAGGGTACAT TCGGATTGTC CACAAGAACC GGGCACAGAC ACTGACCAGA 
GACAGGGCAT TTGAGAGCAC CCTGAAGTCC TGGGAAGACA AGCAGAAGTG CGAGCTGCCC 
CGCCCTCCAT CCTGTTCCCT GCACCCGGAG ATGCTGTCCG AGGAGGAGGC CACCCAGATG 
GACCAGTTTG GGCAGGCAGC AGGGGTCCTC ATTCACAGTA TCCGAGAAAT AGCTCAGTCT 
CACCGGGACA TGGAGCAGGA ACTGGCCCAC GCCGTCAATG CCAGCTCCAA GTCCATGGAC 
CGTGTGTATG GCAAGCCCAG AACCACAGAG GGGCTCAACT GCAGCTTCGT CCTGGAAATG 
GTGAATAACT TCAGAGCTCT GCGCAGTGAG ACGGAGCTGC TGCTGTCTGG GAAGATGGCC 
CTGCAGCTGG ATCCGCCTCA GAAGGAGCAG CTGGGGAGTG CTCTTGCCGA GATGGACCGA 
CAGCTCAGGA GGCTGGCAGA CACCCCGTGG CTCTGCCAGT CCGCAGAGCC CGGCGACGAA 
GAGAGTGTGA TGCTGGATCT TGCCAAGCGC AGTCGCAGTG GTAAATTCCG CCTCGTGACC 
AAGTTTAAAA AGGAGAAAAA CAACAAGAAC AAAGAAGCTC ACAGTAGCCT GGGAGCCCCG 
GTTCACCTCT GGGGGACAGA GGAGGTTGCT GCCTGGCTGG AGCACCTCAG TCTCTGTGAG 
TATAAGGACA TCTTCACACG GCACGACATC CGGGGCTCTG AGCTCCTGCA CCTGGAGCGG 
AGGGACCTCA AGGACCTGGG CGTGACCAAG GTGGGCCACA TGAAGAGGAT CCTGTGTGGC 
ATCAAGGAGC TGAGCCGCAG CGCCCCCGCC GTCGAGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308957.1
CDS	531..2852
Translation	MLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_024453189.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453189.1

ORF Insert Sequence:

ATGCTGGACA GGTGGAGCGT CATGGCATAC GAGGCCAAGC TCCCCCGGCA GGCCTCCTCC 
TCTACCGTCA CCGAAGACTT CAGCGAGGAT TCCGAGGTAC AGCAGATTCT CTTCTATGAA 
GACTCGGTTG CAGCCCACCT TTCTAAAATC CTCACCTCGG ACCAGCACTC GGTGGTCATC 
TCCTCGGCCA AAGTGCTCTG TGAGACGGTG AAGGACTTCG TGGCACGGGT GGGGAAGGCC 
TATGAGAAGA CGACCGAGAG CTCGGAGGAG TCAGAGGTCA TGGCCAAGAA GTGCTCTGTC 
CTGAAAGAGA AGCTGGATTC CCTTCTCAAG ACCTTGGACG ATGAGTCCCA GGCCTCGTCC 
TCTCTGCCCA ACCCGCCCCC CACCATTGCC GAGGAGGCTG AAGATGGAGA TGGGTCGGGC 
AGCATCTGCG GTTCCACCGG AGACCGCTTG GTGGCATCAG CTTGCCCGGC CCGGCCGCAG 
ATATTCCGGC CTCGAGAACA GCTCATGCTG AGAGCCAACA GCCTGAAGAA AGCAATTCGT 
CAGATCATAG AACACACAGA AAAAGCTGTC GATGAGCAGA ATGCCCAGAC CCAGGAGCAG 
GAGGGCTTCG TCCTGGGCCT CTCTGAGTCA GAGGAGAAGA TGGACCACAG AGTGTGCCCA 
CCACTGTCCC ACAGCGAGAG CTTCGGGGTC CCCAAGGGGA GGAGCCAGCG CAAAGTGTCG 
AAATCTCCGT GTGAAAAGCT GATCAGCAAA GGGAGTCTGT CCCTAGGCAG TTCTGCTTCC 
CTTCCGCCCC AGCCGGGAAG CCGGGACGGC CTGCCTGCGC TCAACACCAA GATCCTGTAC 
CCAAATGTCC GGGCTGGAAT GTCTGGTTCC TTACCCGGTG GCTCAGTCAT CAGTCGCCTG 
TTAATTAATG CTGATCCCTT CAACTCTGAA CCAGAAACCC TAGAGTATTA CACGGAGAAA 
TGTGTCATGA ACAACTATTT TGGCATTGGC CTGGATGCGA AGATATCCCT GGACTTTAAC 
AACAAGCGCG ATGAGCACCC AGAGAAGTGC AGGAGCCGAA CCAAGAACAT GATGTGGTAT 
GGAGTTCTTG GAACCAAAGA GTTGCTGCAC AGAACCTACA AGAACCTGGA GCAAAAGGTC 
TTGCTGGAGT GTGACGGGCG ACCCATCCCA CTCCCCAGTC TTCAGGGAAT TGCTGTCCTT 
AACATTCCCA GCTATGCCGG AGGAACCAAC TTCTGGGGGG GTACCAAGGA AGATGATACT 
TTCGCAGCTC CATCATTCGA TGACAAGATT CTGGAGGTGG TCGCCGTGTT CGGCAGCATG 
CAGATGGCCG TCTCTCGAGT CATCAGGCTA CAGCATCATC GGATCGCCCA GTGTCGCACG 
GTGAAGATCT CCATCCTTGG GGATGAGGGC GTGCCTGTGC AGGTGGACGG AGAGGCCTGG 
GTCCAGCCGC CAGGGTACAT TCGGATTGTC CACAAGAACC GGGCACAGAC ACTGACCAGA 
GACAGGGCAT TTGAGAGCAC CCTGAAGTCC TGGGAAGACA AGCAGAAGTG CGAGCTGCCC 
CGCCCTCCAT CCTGTTCCCT GCACCCGGAG ATGCTGTCCG AGGAGGAGGC CACCCAGATG 
GACCAGTTTG GGCAGGCAGC AGGGGTCCTC ATTCACAGTA TCCGAGAAAT AGCTCAGTCT 
CACCGGGACA TGGAGCAGGA ACTGGCCCAC GCCGTCAATG CCAGCTCCAA GTCCATGGAC 
CGTGTGTATG GCAAGCCCAG AACCACAGAG GGGCTCAACT GCAGCTTCGT CCTGGAAATG 
GTGAATAACT TCAGAGCTCT GCGCAGTGAG ACGGAGCTGC TGCTGTCTGG GAAGATGGCC 
CTGCAGCTGG ATCCGCCTCA GAAGGAGCAG CTGGGGAGTG CTCTTGCCGA GATGGACCGA 
CAGCTCAGGA GGCTGGCAGA CACCCCGTGG CTCTGCCAGT CCGCAGAGCC CGGCGACGAA 
GAGAGTGTGA TGCTGGATCT TGCCAAGCGC AGTCGCAGTG GTAAATTCCG CCTCGTGACC 
AAGTTTAAAA AGGAGAAAAA CAACAAGAAC AAAGAAGCTC ACAGTAGCCT GGGAGCCCCG 
GTTCACCTCT GGGGGACAGA GGAGGTTGCT GCCTGGCTGG AGCACCTCAG TCTCTGTGAG 
TATAAGGACA TCTTCACACG GCACGACATC CGGGGCTCTG AGCTCCTGCA CCTGGAGCGG 
AGGGACCTCA AGGACCTGGG CGTGACCAAG GTGGGCCACA TGAAGAGGAT CCTGTGTGGC 
ATCAAGGAGC TGAGCCGCAG CGCCCCCGCC GTCGAGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( NM_001377259.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001364188.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 3, mRNA.

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CloneID	OHu36703
Clone ID Related Accession (Same CDS sequence)	NM_001377259.1 , XM_011512035.1 , XM_017005140.1 , XM_011512040.2 , XM_011512037.2 , XM_005246108.1
Accession Version	NM_001377259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC013726.7 and AC019221.4. On Dec 20, 2019 this sequence version replaced XM_005246108.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.120094.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAAGATT GGATTGCAGC ATTAAAGACT GTGCAGAACA GGGAGCACTT TGAGCCCACC 
CAGTACAGCA TGGACCACTT CTCAGGGATG CACAATTGGT ACGCCTGTTC CCACGCGAGG 
CCGACCTACT GCAATGTGTG CCGTGAGGCT CTGTCTGGGG TCACGTCGCA CGGGCTGTCC 
TGCGAGGTGT GCAAATTTAA GGCCCACAAG CGCTGTGCTG TGCGTGCAAC CAATAACTGC 
AAGTGGACCA CACTGGCCTC GATCGGGAAG GACATCATTG AAGATGCAGA TGGGATTGCA 
ATGCCCCACC AGTGGTTGGA AGGAAACCTA CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC 
AAGACCTGTG GCAGTGTGCT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CGTGTAAAGA ATCCTTGCTG ACCAAGTGCC CACTTGGCCT GTGCAAAGTG 
TCAGTCATCC CACCCACGGC TCTCAACAGC ATCGACTCCG ATGGGTTCTG GAAGGCCAGC 
TGTCCTCCTT CTTGCACAAG CCCACTGTTG GTCTTCGTCA ATTCAAAAAG TGGGGACAAC 
CAGGGTGTGA AGTTCCTCAG AAGATTCAAA CAGCTACTAA ACCCCGCCCA GGTCTTCGAC 
CTCATGAACG GAGGCCCACA CCTCGGCTTA CGGTTATTCC AGAAGTTTGA CACATTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT GGCTGGGTCC TCTCCGAAAT CGACAGCCTC 
AACCTTCATA AACAGTGTCA GCTGGGAGTG CTGCCGCTCG GCACAGGGAA CGACTTGGCC 
CGAGTACTGG GCTGGGGCTC AGCCTGCGAT GACGACACCC AGCTCCCCCA GATCTTGGAG 
AAGTTGGAGA GAGCCAGCAC CAAGATGCTG GACAGGTGGA GCGTCATGGC ATACGAGGCC 
AAGCTCCCCC GGCAGGCCTC CTCCTCTACC GTCACCGAAG ACTTCAGCGA GGATTCCGAG 
GTACAGCAGA TTCTCTTCTA TGAAGACTCG GTTGCAGCCC ACCTTTCTAA AATCCTCACC 
TCGGACCAGC ACTCGGTGGT CATCTCCTCG GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC 
TTCGTGGCAC GGGTGGGGAA GGCCTATGAG AAGACGACCG AGAGCTCGGA GGAGTCAGAG 
GTCATGGCCA AGAAGTGCTC TGTCCTGAAA GAGAAGCTGG ATTCCCTTCT CAAGACCTTG 
GACGATGAGT CCCAGGCCTC GTCCTCTCTG CCCAACCCGC CCCCCACCAT TGCCGAGGAG 
GCTGAAGATG GAGATGGGTC GGGCAGCATC TGCGGTTCCA CCGGAGACCG CTTGGTGGCA 
TCAGCTTGCC CGGCCCGGCC GCAGATATTC CGGCCTCGAG AACAGCTCAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGTCAGATC ATAGAACACA CAGAAAAAGC TGTCGATGAG 
CAGAATGCCC AGACCCAGGA GCAGGAGGGC TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG 
AAGATGGACC ACAGAGTGTG CCCACCACTG TCCCACAGCG AGAGCTTCGG GGTCCCCAAG 
GGGAGGAGCC AGCGCAAAGT GTCGAAATCT CCGTGTGAAA AGCTGATCAG CAAAGGGAGT 
CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT 
GCGCTCAACA CCAAGATCCT GTACCCAAAT GTCCGGGCTG GAATGTCTGG TTCCTTACCC 
GGTGGCTCAG TCATCAGTCG CCTGTTAATT AATGCTGATC CCTTCAACTC TGAACCAGAA 
ACCCTAGAGT ATTACACGGA GAAATGTGTC ATGAACAACT ATTTTGGCAT TGGCCTGGAT 
GCGAAGATAT CCCTGGACTT TAACAACAAG CGCGATGAGC ACCCAGAGAA GTGCAGGAGC 
CGAACCAAGA ACATGATGTG GTATGGAGTT CTTGGAACCA AAGAGTTGCT GCACAGAACC 
TACAAGAACC TGGAGCAAAA GGTCTTGCTG GAGTGTGACG GGCGACCCAT CCCACTCCCC 
AGTCTTCAGG GAATTGCTGT CCTTAACATT CCCAGCTATG CCGGAGGAAC CAACTTCTGG 
GGGGGTACCA AGGAAGATGA TACTTTCGCA GCTCCATCAT TCGATGACAA GATTCTGGAG 
GTGGTCGCCG TGTTCGGCAG CATGCAGATG GCCGTCTCTC GAGTCATCAG GCTACAGCAT 
CATCGGATCG CCCAGTGTCG CACGGTGAAG ATCTCCATCC TTGGGGATGA GGGCGTGCCT 
GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG CCGCCAGGGT ACATTCGGAT TGTCCACAAG 
AACCGGGCAC AGACACTGAC CAGAGACAGG GCATTTGAGA GCACCCTGAA GTCCTGGGAA 
GACAAGCAGA AGTGCGAGCT GCCCCGCCCT CCATCCTGTT CCCTGCACCC GGAGATGCTG 
TCCGAGGAGG AGGCCACCCA GATGGACCAG TTTGGGCAGG CAGCAGGGGT CCTCATTCAC 
AGTATCCGAG AAATAGCTCA GTCTCACCGG GACATGGAGC AGGAACTGGC CCACGCCGTC 
AATGCCAGCT CCAAGTCCAT GGACCGTGTG TATGGCAAGC CCAGAACCAC AGAGGGGCTC 
AACTGCAGCT TCGTCCTGGA AATGGTGAAT AACTTCAGAG CTCTGCGCAG TGAGACGGAG 
CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG CTGGATCCGC CTCAGAAGGA GCAGCTGGGG 
AGTGCTCTTG CCGAGATGGA CCGACAGCTC AGGAGGCTGG CAGACACCCC GTGGCTCTGC 
CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT GTGATGCTGG ATCTTGCCAA GCGCAGTCGC 
AGTGGTAAAT TCCGCCTCGT GACCAAGTTT AAAAAGGAGA AAAACAACAA GAACAAAGAA 
GCTCACAGTA GCCTGGGAGC CCCGGTTCAC CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG 
CTGGAGCACC TCAGTCTCTG TGAGTATAAG GACATCTTCA CACGGCACGA CATCCGGGGC 
TCTGAGCTCC TGCACCTGGA GCGGAGGGAC CTCAAGGACC TGGGCGTGAC CAAGGTGGGC 
CACATGAAGA GGATCCTGTG TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001364188.1
CDS	262..3507
Translation	MEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	NM_001377259.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377259.1

ORF Insert Sequence:

ATGGAAGATT GGATTGCAGC ATTAAAGACT GTGCAGAACA GGGAGCACTT TGAGCCCACC 
CAGTACAGCA TGGACCACTT CTCAGGGATG CACAATTGGT ACGCCTGTTC CCACGCGAGG 
CCGACCTACT GCAATGTGTG CCGTGAGGCT CTGTCTGGGG TCACGTCGCA CGGGCTGTCC 
TGCGAGGTGT GCAAATTTAA GGCCCACAAG CGCTGTGCTG TGCGTGCAAC CAATAACTGC 
AAGTGGACCA CACTGGCCTC GATCGGGAAG GACATCATTG AAGATGCAGA TGGGATTGCA 
ATGCCCCACC AGTGGTTGGA AGGAAACCTA CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC 
AAGACCTGTG GCAGTGTGCT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CGTGTAAAGA ATCCTTGCTG ACCAAGTGCC CACTTGGCCT GTGCAAAGTG 
TCAGTCATCC CACCCACGGC TCTCAACAGC ATCGACTCCG ATGGGTTCTG GAAGGCCAGC 
TGTCCTCCTT CTTGCACAAG CCCACTGTTG GTCTTCGTCA ATTCAAAAAG TGGGGACAAC 
CAGGGTGTGA AGTTCCTCAG AAGATTCAAA CAGCTACTAA ACCCCGCCCA GGTCTTCGAC 
CTCATGAACG GAGGCCCACA CCTCGGCTTA CGGTTATTCC AGAAGTTTGA CACATTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT GGCTGGGTCC TCTCCGAAAT CGACAGCCTC 
AACCTTCATA AACAGTGTCA GCTGGGAGTG CTGCCGCTCG GCACAGGGAA CGACTTGGCC 
CGAGTACTGG GCTGGGGCTC AGCCTGCGAT GACGACACCC AGCTCCCCCA GATCTTGGAG 
AAGTTGGAGA GAGCCAGCAC CAAGATGCTG GACAGGTGGA GCGTCATGGC ATACGAGGCC 
AAGCTCCCCC GGCAGGCCTC CTCCTCTACC GTCACCGAAG ACTTCAGCGA GGATTCCGAG 
GTACAGCAGA TTCTCTTCTA TGAAGACTCG GTTGCAGCCC ACCTTTCTAA AATCCTCACC 
TCGGACCAGC ACTCGGTGGT CATCTCCTCG GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC 
TTCGTGGCAC GGGTGGGGAA GGCCTATGAG AAGACGACCG AGAGCTCGGA GGAGTCAGAG 
GTCATGGCCA AGAAGTGCTC TGTCCTGAAA GAGAAGCTGG ATTCCCTTCT CAAGACCTTG 
GACGATGAGT CCCAGGCCTC GTCCTCTCTG CCCAACCCGC CCCCCACCAT TGCCGAGGAG 
GCTGAAGATG GAGATGGGTC GGGCAGCATC TGCGGTTCCA CCGGAGACCG CTTGGTGGCA 
TCAGCTTGCC CGGCCCGGCC GCAGATATTC CGGCCTCGAG AACAGCTCAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGTCAGATC ATAGAACACA CAGAAAAAGC TGTCGATGAG 
CAGAATGCCC AGACCCAGGA GCAGGAGGGC TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG 
AAGATGGACC ACAGAGTGTG CCCACCACTG TCCCACAGCG AGAGCTTCGG GGTCCCCAAG 
GGGAGGAGCC AGCGCAAAGT GTCGAAATCT CCGTGTGAAA AGCTGATCAG CAAAGGGAGT 
CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT 
GCGCTCAACA CCAAGATCCT GTACCCAAAT GTCCGGGCTG GAATGTCTGG TTCCTTACCC 
GGTGGCTCAG TCATCAGTCG CCTGTTAATT AATGCTGATC CCTTCAACTC TGAACCAGAA 
ACCCTAGAGT ATTACACGGA GAAATGTGTC ATGAACAACT ATTTTGGCAT TGGCCTGGAT 
GCGAAGATAT CCCTGGACTT TAACAACAAG CGCGATGAGC ACCCAGAGAA GTGCAGGAGC 
CGAACCAAGA ACATGATGTG GTATGGAGTT CTTGGAACCA AAGAGTTGCT GCACAGAACC 
TACAAGAACC TGGAGCAAAA GGTCTTGCTG GAGTGTGACG GGCGACCCAT CCCACTCCCC 
AGTCTTCAGG GAATTGCTGT CCTTAACATT CCCAGCTATG CCGGAGGAAC CAACTTCTGG 
GGGGGTACCA AGGAAGATGA TACTTTCGCA GCTCCATCAT TCGATGACAA GATTCTGGAG 
GTGGTCGCCG TGTTCGGCAG CATGCAGATG GCCGTCTCTC GAGTCATCAG GCTACAGCAT 
CATCGGATCG CCCAGTGTCG CACGGTGAAG ATCTCCATCC TTGGGGATGA GGGCGTGCCT 
GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG CCGCCAGGGT ACATTCGGAT TGTCCACAAG 
AACCGGGCAC AGACACTGAC CAGAGACAGG GCATTTGAGA GCACCCTGAA GTCCTGGGAA 
GACAAGCAGA AGTGCGAGCT GCCCCGCCCT CCATCCTGTT CCCTGCACCC GGAGATGCTG 
TCCGAGGAGG AGGCCACCCA GATGGACCAG TTTGGGCAGG CAGCAGGGGT CCTCATTCAC 
AGTATCCGAG AAATAGCTCA GTCTCACCGG GACATGGAGC AGGAACTGGC CCACGCCGTC 
AATGCCAGCT CCAAGTCCAT GGACCGTGTG TATGGCAAGC CCAGAACCAC AGAGGGGCTC 
AACTGCAGCT TCGTCCTGGA AATGGTGAAT AACTTCAGAG CTCTGCGCAG TGAGACGGAG 
CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG CTGGATCCGC CTCAGAAGGA GCAGCTGGGG 
AGTGCTCTTG CCGAGATGGA CCGACAGCTC AGGAGGCTGG CAGACACCCC GTGGCTCTGC 
CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT GTGATGCTGG ATCTTGCCAA GCGCAGTCGC 
AGTGGTAAAT TCCGCCTCGT GACCAAGTTT AAAAAGGAGA AAAACAACAA GAACAAAGAA 
GCTCACAGTA GCCTGGGAGC CCCGGTTCAC CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG 
CTGGAGCACC TCAGTCTCTG TGAGTATAAG GACATCTTCA CACGGCACGA CATCCGGGGC 
TCTGAGCTCC TGCACCTGGA GCGGAGGGAC CTCAAGGACC TGGGCGTGAC CAAGGTGGGC 
CACATGAAGA GGATCCTGTG TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_017005139.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860628.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X7, mRNA.

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CloneID	OHu90009
Clone ID Related Accession (Same CDS sequence)	XM_017005139.1
Accession Version	XM_017005139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAACT CCAAGAGCTT GTTTGTGTGG TGGTGTCTAT TTTTGTGTTT TGTGTGGTGC 
TCTGTGAGGA AGTCTGTTGT CTACGCACAT CCCAGCTGCT TTGAATGGTC TGTCTCTGGG 
GGCTTCCAGG TCATAACTCC ATGCAGGAAG CTCATCTTGT GTGCTGATAA CAGAAAAGAA 
ATGGAAGATT GGATTGCAGC ATTAAAGACT GTGCAGAACA GGGAGCACTT TGAGCCCACC 
CAGTACAGCA TGGACCACTT CTCAGGGATG CACAATTGGT ACGCCTGTTC CCACGCGAGG 
CCGACCTACT GCAATGTGTG CCGTGAGGCT CTGTCTGGGG TCACGTCGCA CGGGCTGTCC 
TGCGAGGTGT GCAAATTTAA GGCCCACAAG CGCTGTGCTG TGCGTGCAAC CAATAACTGC 
AAGTGGACCA CACTGGCCTC GATCGGGAAG GACATCATTG AAGATGCAGA TGGGATTGCA 
ATGCCCCACC AGTGGTTGGA AGGAAACCTA CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC 
AAGACCTGTG GCAGTGTGCT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CGTGTAAAGA ATCCTTGCTG ACCAAGTGCC CACTTGGCCT GTGCAAAGTG 
TCAGTCATCC CACCCACGGC TCTCAACAGC ATCGACTCCG ATGGGTTCTG GAAGGCCAGC 
TGTCCTCCTT CTTGCACAAG CCCACTGTTG GTCTTCGTCA ATTCAAAAAG TGGGGACAAC 
CAGGGTGTGA AGTTCCTCAG AAGATTCAAA CAGCTACTAA ACCCCGCCCA GGTCTTCGAC 
CTCATGAACG GAGGCCCACA CCTCGGCTTA CGGTTATTCC AGAAGTTTGA CACATTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT GGCTGGGTCC TCTCCGAAAT CGACAGCCTC 
AACCTTCATA AACAGTGTCA GCTGGGAGTG CTGCCGCTCG GCACAGGGAA CGACTTGGCC 
CGAGTACTGG GCTGGGGCTC AGCCTGCGAT GACGACACCC AGCTCCCCCA GATCTTGGAG 
AAGTTGGAGA GAGCCAGCAC CAAGATGCTG GACAGGTGGA GCGTCATGGC ATACGAGGCC 
AAGCTCCCCC GGCAGGCCTC CTCCTCTACC GTCACCGAAG ACTTCAGCGA GGATTCCGAG 
GTACAGCAGA TTCTCTTCTA TGAAGACTCG GTTGCAGCCC ACCTTTCTAA AATCCTCACC 
TCGGACCAGC ACTCGGTGGT CATCTCCTCG GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC 
TTCGTGGCAC GGGTGGGGAA GGCCTATGAG AAGACGACCG AGAGCTCGGA GGAGTCAGAG 
GTCATGGCCA AGAAGTGCTC TGTCCTGAAA GAGAAGCTGG ATTCCCTTCT CAAGACCTTG 
GACGATGAGT CCCAGGCCTC GTCCTCTCTG CCCAACCCGC CCCCCACCAT TGCCGAGGAG 
GCTGAAGATG GAGATGGGTC GGGCAGCATC TGCGGTTCCA CCGGAGACCG CTTGGTGGCA 
TCAGCTTGCC CGGCCCGGCC GCAGATATTC CGGCCTCGAG AACAGCTCAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGTCAGATC ATAGAACACA CAGAAAAAGC TGTCGATGAG 
CAGAATGCCC AGACCCAGGA GCAGGAGGGC TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG 
AAGATGGACC ACAGAGTGTG CCCACCACTG TCCCACAGCG AGAGCTTCGG GGTCCCCAAG 
GGGAGGAGCC AGCGCAAAGT GTCGAAATCT CCGTGTGAAA AGCTGATCAG CAAAGGGAGT 
CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT 
GCGCTCAACA CCAAGATCCT GTACCCAAAT GTCCGGGCTG GAATGTCTGG TTCCTTACCC 
GGTGGCTCAG TCATCAGTCG CCTGTTAATT AATGCTGATC CCTTCAACTC TGAACCAGAA 
ACCCTAGAGT ATTACACGGA GAAATGTGTC ATGAACAACT ATTTTGGCAT TGGCCTGGAT 
GCGAAGATAT CCCTGGACTT TAACAACAAG CGCGATGAGC ACCCAGAGAA GTGCAGGAGC 
CGAACCAAGA ACATGATGTG GTATGGAGTT CTTGGAACCA AAGAGTTGCT GCACAGAACC 
TACAAGAACC TGGAGCAAAA GGTCTTGCTG GAGTGTGACG GGCGACCCAT CCCACTCCCC 
AGTCTTCAGG GAATTGCTGT CCTTAACATT CCCAGCTATG CCGGAGGAAC CAACTTCTGG 
GGGGGTACCA AGGAAGATGA TACTTTCGCA GCTCCATCAT TCGATGACAA GATTCTGGAG 
GTGGTCGCCG TGTTCGGCAG CATGCAGATG GCCGTCTCTC GAGTCATCAG GCTACAGCAT 
CATCGGATCG CCCAGTGTCG CACGGTGAAG ATCTCCATCC TTGGGGATGA GGGCGTGCCT 
GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG CCGCCAGGGT ACATTCGGAT TGTCCACAAG 
AACCGGGCAC AGACACTGAC CAGAGACAGG GCATTTGAGA GCACCCTGAA GTCCTGGGAA 
GACAAGCAGA AGTGCGAGCT GCCCCGCCCT CCATCCTGTT CCCTGCACCC GGAGATGCTG 
TCCGAGGAGG AGGCCACCCA GATGGACCAG TTTGGGCAGG CAGCAGGGGT CCTCATTCAC 
AGTATCCGAG AAATAGCTCA GTCTCACCGG GACATGGAGC AGGAACTGGC CCACGCCGTC 
AATGCCAGCT CCAAGTCCAT GGACCGTGTG TATGGCAAGC CCAGAACCAC AGAGGGGCTC 
AACTGCAGCT TCGTCCTGGA AATGGTGAAT AACTTCAGAG CTCTGCGCAG TGAGACGGAG 
CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG CTGGATCCGC CTCAGAAGGA GCAGCTGGGG 
AGTGCTCTTG CCGAGATGGA CCGACAGCTC AGGAGGCTGG CAGACACCCC GTGGCTCTGC 
CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT GTGATGCTGG ATCTTGCCAA GCGCAGTCGC 
AGTGGTAAAT TCCGCCTCGT GACCAAGTTT AAAAAGGAGA AAAACAACAA GAACAAAGAA 
GCTCACAGTA GCCTGGGAGC CCCGGTTCAC CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG 
CTGGAGCACC TCAGTCTCTG TGAGTATAAG GACATCTTCA CACGGCACGA CATCCGGGGC 
TCTGAGCTCC TGCACCTGGA GCGGAGGGAC CTCAAGGACC TGGGCGTGAC CAAGGTGGGC 
CACATGAAGA GGATCCTGTG TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860628.1
CDS	62..3487
Translation	MGNSKSLFVWWCLFLCFVWCSVRKSVVYAHPSCFEWSVSGGFQVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_017005139.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005139.1

ORF Insert Sequence:

ATGGGGAACT CCAAGAGCTT GTTTGTGTGG TGGTGTCTAT TTTTGTGTTT TGTGTGGTGC 
TCTGTGAGGA AGTCTGTTGT CTACGCACAT CCCAGCTGCT TTGAATGGTC TGTCTCTGGG 
GGCTTCCAGG TCATAACTCC ATGCAGGAAG CTCATCTTGT GTGCTGATAA CAGAAAAGAA 
ATGGAAGATT GGATTGCAGC ATTAAAGACT GTGCAGAACA GGGAGCACTT TGAGCCCACC 
CAGTACAGCA TGGACCACTT CTCAGGGATG CACAATTGGT ACGCCTGTTC CCACGCGAGG 
CCGACCTACT GCAATGTGTG CCGTGAGGCT CTGTCTGGGG TCACGTCGCA CGGGCTGTCC 
TGCGAGGTGT GCAAATTTAA GGCCCACAAG CGCTGTGCTG TGCGTGCAAC CAATAACTGC 
AAGTGGACCA CACTGGCCTC GATCGGGAAG GACATCATTG AAGATGCAGA TGGGATTGCA 
ATGCCCCACC AGTGGTTGGA AGGAAACCTA CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC 
AAGACCTGTG GCAGTGTGCT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CGTGTAAAGA ATCCTTGCTG ACCAAGTGCC CACTTGGCCT GTGCAAAGTG 
TCAGTCATCC CACCCACGGC TCTCAACAGC ATCGACTCCG ATGGGTTCTG GAAGGCCAGC 
TGTCCTCCTT CTTGCACAAG CCCACTGTTG GTCTTCGTCA ATTCAAAAAG TGGGGACAAC 
CAGGGTGTGA AGTTCCTCAG AAGATTCAAA CAGCTACTAA ACCCCGCCCA GGTCTTCGAC 
CTCATGAACG GAGGCCCACA CCTCGGCTTA CGGTTATTCC AGAAGTTTGA CACATTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT GGCTGGGTCC TCTCCGAAAT CGACAGCCTC 
AACCTTCATA AACAGTGTCA GCTGGGAGTG CTGCCGCTCG GCACAGGGAA CGACTTGGCC 
CGAGTACTGG GCTGGGGCTC AGCCTGCGAT GACGACACCC AGCTCCCCCA GATCTTGGAG 
AAGTTGGAGA GAGCCAGCAC CAAGATGCTG GACAGGTGGA GCGTCATGGC ATACGAGGCC 
AAGCTCCCCC GGCAGGCCTC CTCCTCTACC GTCACCGAAG ACTTCAGCGA GGATTCCGAG 
GTACAGCAGA TTCTCTTCTA TGAAGACTCG GTTGCAGCCC ACCTTTCTAA AATCCTCACC 
TCGGACCAGC ACTCGGTGGT CATCTCCTCG GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC 
TTCGTGGCAC GGGTGGGGAA GGCCTATGAG AAGACGACCG AGAGCTCGGA GGAGTCAGAG 
GTCATGGCCA AGAAGTGCTC TGTCCTGAAA GAGAAGCTGG ATTCCCTTCT CAAGACCTTG 
GACGATGAGT CCCAGGCCTC GTCCTCTCTG CCCAACCCGC CCCCCACCAT TGCCGAGGAG 
GCTGAAGATG GAGATGGGTC GGGCAGCATC TGCGGTTCCA CCGGAGACCG CTTGGTGGCA 
TCAGCTTGCC CGGCCCGGCC GCAGATATTC CGGCCTCGAG AACAGCTCAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGTCAGATC ATAGAACACA CAGAAAAAGC TGTCGATGAG 
CAGAATGCCC AGACCCAGGA GCAGGAGGGC TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG 
AAGATGGACC ACAGAGTGTG CCCACCACTG TCCCACAGCG AGAGCTTCGG GGTCCCCAAG 
GGGAGGAGCC AGCGCAAAGT GTCGAAATCT CCGTGTGAAA AGCTGATCAG CAAAGGGAGT 
CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT 
GCGCTCAACA CCAAGATCCT GTACCCAAAT GTCCGGGCTG GAATGTCTGG TTCCTTACCC 
GGTGGCTCAG TCATCAGTCG CCTGTTAATT AATGCTGATC CCTTCAACTC TGAACCAGAA 
ACCCTAGAGT ATTACACGGA GAAATGTGTC ATGAACAACT ATTTTGGCAT TGGCCTGGAT 
GCGAAGATAT CCCTGGACTT TAACAACAAG CGCGATGAGC ACCCAGAGAA GTGCAGGAGC 
CGAACCAAGA ACATGATGTG GTATGGAGTT CTTGGAACCA AAGAGTTGCT GCACAGAACC 
TACAAGAACC TGGAGCAAAA GGTCTTGCTG GAGTGTGACG GGCGACCCAT CCCACTCCCC 
AGTCTTCAGG GAATTGCTGT CCTTAACATT CCCAGCTATG CCGGAGGAAC CAACTTCTGG 
GGGGGTACCA AGGAAGATGA TACTTTCGCA GCTCCATCAT TCGATGACAA GATTCTGGAG 
GTGGTCGCCG TGTTCGGCAG CATGCAGATG GCCGTCTCTC GAGTCATCAG GCTACAGCAT 
CATCGGATCG CCCAGTGTCG CACGGTGAAG ATCTCCATCC TTGGGGATGA GGGCGTGCCT 
GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG CCGCCAGGGT ACATTCGGAT TGTCCACAAG 
AACCGGGCAC AGACACTGAC CAGAGACAGG GCATTTGAGA GCACCCTGAA GTCCTGGGAA 
GACAAGCAGA AGTGCGAGCT GCCCCGCCCT CCATCCTGTT CCCTGCACCC GGAGATGCTG 
TCCGAGGAGG AGGCCACCCA GATGGACCAG TTTGGGCAGG CAGCAGGGGT CCTCATTCAC 
AGTATCCGAG AAATAGCTCA GTCTCACCGG GACATGGAGC AGGAACTGGC CCACGCCGTC 
AATGCCAGCT CCAAGTCCAT GGACCGTGTG TATGGCAAGC CCAGAACCAC AGAGGGGCTC 
AACTGCAGCT TCGTCCTGGA AATGGTGAAT AACTTCAGAG CTCTGCGCAG TGAGACGGAG 
CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG CTGGATCCGC CTCAGAAGGA GCAGCTGGGG 
AGTGCTCTTG CCGAGATGGA CCGACAGCTC AGGAGGCTGG CAGACACCCC GTGGCTCTGC 
CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT GTGATGCTGG ATCTTGCCAA GCGCAGTCGC 
AGTGGTAAAT TCCGCCTCGT GACCAAGTTT AAAAAGGAGA AAAACAACAA GAACAAAGAA 
GCTCACAGTA GCCTGGGAGC CCCGGTTCAC CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG 
CTGGAGCACC TCAGTCTCTG TGAGTATAAG GACATCTTCA CACGGCACGA CATCCGGGGC 
TCTGAGCTCC TGCACCTGGA GCGGAGGGAC CTCAAGGACC TGGGCGTGAC CAAGGTGGGC 
CACATGAAGA GGATCCTGTG TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( NM_003648.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003639.2 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14020
Clone ID Related Accession (Same CDS sequence)	NM_003648.3 , NM_003648.2
Accession Version	NM_003648.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D73409.1, D63479.3, BC006561.2 and AC019221.4. On Jun 2, 2019 this sequence version replaced NM_003648.2. Transcript Variant: This variant (1) differs in the 5' UTR and uses a different in-frame start codon, compared to variant 2. The encoded isoform (1) has a shorter, distinct N-terminus, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D73409.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATATGT TTCTGTACTT TCAGACCATC ATCAAAGAGG GGATGCTGAC CAAACAGAAC 
AATTCATTCC AGCGATCAAA AAGGAGATAC TTTAAGCTTC GAGGGCGAAC GCTTTACTAT 
GCCAAAACGG CAAAGTCAAT CATATTTGAT GAGGTGGATC TGACAGATGC CAGCGTAGCT 
GAATCCAGTA CCAAAAACGT CAACAACAGT TTTACGGTCA TAACTCCATG CAGGAAGCTC 
ATCTTGTGTG CTGATAACAG AAAAGAAATG GAAGATTGGA TTGCAGCATT AAAGACTGTG 
CAGAACAGGG AGCACTTTGA GCCCACCCAG TACAGCATGG ACCACTTCTC AGGGATGCAC 
AATTGGTACG CCTGTTCCCA CGCGAGGCCG ACCTACTGCA ATGTGTGCCG TGAGGCTCTG 
TCTGGGGTCA CGTCGCACGG GCTGTCCTGC GAGGTGTGCA AATTTAAGGC CCACAAGCGC 
TGTGCTGTGC GTGCAACCAA TAACTGCAAG TGGACCACAC TGGCCTCGAT CGGGAAGGAC 
ATCATTGAAG ATGCAGATGG GATTGCAATG CCCCACCAGT GGTTGGAAGG AAACCTACCT 
GTGAGCGCCA AGTGCACTGT GTGCGACAAG ACCTGTGGCA GTGTGCTGCG CCTGCAGGAC 
TGGCGCTGCC TCTGGTGCAA GGCCATGGTT CACACATCGT GTAAAGAATC CTTGCTGACC 
AAGTGCCCAC TTGGCCTGTG CAAAGTGTCA GTCATCCCAC CCACGGCTCT CAACAGCATC 
GACTCCGATG GGTTCTGGAA GGCCAGCTGT CCTCCTTCTT GCACAAGCCC ACTGTTGGTC 
TTCGTCAATT CAAAAAGTGG GGACAACCAG GGTGTGAAGT TCCTCAGAAG ATTCAAACAG 
CTACTAAACC CCGCCCAGGT CTTCGACCTC ATGAACGGAG GCCCACACCT CGGCTTACGG 
TTATTCCAGA AGTTTGACAC ATTCCGGATT CTGGTTTGTG GCGGGGATGG AAGTGTTGGC 
TGGGTCCTCT CCGAAATCGA CAGCCTCAAC CTTCATAAAC AGTGTCAGCT GGGAGTGCTG 
CCGCTCGGCA CAGGGAACGA CTTGGCCCGA GTACTGGGCT GGGGCTCAGC CTGCGATGAC 
GACACCCAGC TCCCCCAGAT CTTGGAGAAG TTGGAGAGAG CCAGCACCAA GATGCTGGAC 
AGGTGGAGCG TCATGGCATA CGAGGCCAAG CTCCCCCGGC AGGCCTCCTC CTCTACCGTC 
ACCGAAGACT TCAGCGAGGA TTCCGAGGTA CAGCAGATTC TCTTCTATGA AGACTCGGTT 
GCAGCCCACC TTTCTAAAAT CCTCACCTCG GACCAGCACT CGGTGGTCAT CTCCTCGGCC 
AAAGTGCTCT GTGAGACGGT GAAGGACTTC GTGGCACGGG TGGGGAAGGC CTATGAGAAG 
ACGACCGAGA GCTCGGAGGA GTCAGAGGTC ATGGCCAAGA AGTGCTCTGT CCTGAAAGAG 
AAGCTGGATT CCCTTCTCAA GACCTTGGAC GATGAGTCCC AGGCCTCGTC CTCTCTGCCC 
AACCCGCCCC CCACCATTGC CGAGGAGGCT GAAGATGGAG ATGGGTCGGG CAGCATCTGC 
GGTTCCACCG GAGACCGCTT GGTGGCATCA GCTTGCCCGG CCCGGCCGCA GATATTCCGG 
CCTCGAGAAC AGCTCATGCT GAGAGCCAAC AGCCTGAAGA AAGCAATTCG TCAGATCATA 
GAACACACAG AAAAAGCTGT CGATGAGCAG AATGCCCAGA CCCAGGAGCA GGAGGGCTTC 
GTCCTGGGCC TCTCTGAGTC AGAGGAGAAG ATGGACCACA GAGTGTGCCC ACCACTGTCC 
CACAGCGAGA GCTTCGGGGT CCCCAAGGGG AGGAGCCAGC GCAAAGTGTC GAAATCTCCG 
TGTGAAAAGC TGATCAGCAA AGGGAGTCTG TCCCTAGGCA GTTCTGCTTC CCTTCCGCCC 
CAGCCGGGAA GCCGGGACGG CCTGCCTGCG CTCAACACCA AGATCCTGTA CCCAAATGTC 
CGGGCTGGAA TGTCTGGTTC CTTACCCGGT GGCTCAGTCA TCAGTCGCCT GTTAATTAAT 
GCTGATCCCT TCAACTCTGA ACCAGAAACC CTAGAGTATT ACACGGAGAA ATGTGTCATG 
AACAACTATT TTGGCATTGG CCTGGATGCG AAGATATCCC TGGACTTTAA CAACAAGCGC 
GATGAGCACC CAGAGAAGTG CAGGAGCCGA ACCAAGAACA TGATGTGGTA TGGAGTTCTT 
GGAACCAAAG AGTTGCTGCA CAGAACCTAC AAGAACCTGG AGCAAAAGGT CTTGCTGGAG 
TGTGACGGGC GACCCATCCC ACTCCCCAGT CTTCAGGGAA TTGCTGTCCT TAACATTCCC 
AGCTATGCCG GAGGAACCAA CTTCTGGGGG GGTACCAAGG AAGATGATAC TTTCGCAGCT 
CCATCATTCG ATGACAAGAT TCTGGAGGTG GTCGCCGTGT TCGGCAGCAT GCAGATGGCC 
GTCTCTCGAG TCATCAGGCT ACAGCATCAT CGGATCGCCC AGTGTCGCAC GGTGAAGATC 
TCCATCCTTG GGGATGAGGG CGTGCCTGTG CAGGTGGACG GAGAGGCCTG GGTCCAGCCG 
CCAGGGTACA TTCGGATTGT CCACAAGAAC CGGGCACAGA CACTGACCAG AGACAGGGCA 
TTTGAGAGCA CCCTGAAGTC CTGGGAAGAC AAGCAGAAGT GCGAGCTGCC CCGCCCTCCA 
TCCTGTTCCC TGCACCCGGA GATGCTGTCC GAGGAGGAGG CCACCCAGAT GGACCAGTTT 
GGGCAGGCAG CAGGGGTCCT CATTCACAGT ATCCGAGAAA TAGCTCAGTC TCACCGGGAC 
ATGGAGCAGG AACTGGCCCA CGCCGTCAAT GCCAGCTCCA AGTCCATGGA CCGTGTGTAT 
GGCAAGCCCA GAACCACAGA GGGGCTCAAC TGCAGCTTCG TCCTGGAAAT GGTGAATAAC 
TTCAGAGCTC TGCGCAGTGA GACGGAGCTG CTGCTGTCTG GGAAGATGGC CCTGCAGCTG 
GATCCGCCTC AGAAGGAGCA GCTGGGGAGT GCTCTTGCCG AGATGGACCG ACAGCTCAGG 
AGGCTGGCAG ACACCCCGTG GCTCTGCCAG TCCGCAGAGC CCGGCGACGA AGAGAGTGTG 
ATGCTGGATC TTGCCAAGCG CAGTCGCAGT GGTAAATTCC GCCTCGTGAC CAAGTTTAAA 
AAGGAGAAAA ACAACAAGAA CAAAGAAGCT CACAGTAGCC TGGGAGCCCC GGTTCACCTC 
TGGGGGACAG AGGAGGTTGC TGCCTGGCTG GAGCACCTCA GTCTCTGTGA GTATAAGGAC 
ATCTTCACAC GGCACGACAT CCGGGGCTCT GAGCTCCTGC ACCTGGAGCG GAGGGACCTC 
AAGGACCTGG GCGTGACCAA GGTGGGCCAC ATGAAGAGGA TCCTGTGTGG CATCAAGGAG 
CTGAGCCGCA GCGCCCCCGC CGTCGAGGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003639.2
CDS	80..3592
Translation	MNMFLYFQTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	NM_003648.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003648.3

ORF Insert Sequence:

ATGAATATGT TTCTGTACTT TCAGACCATC ATCAAAGAGG GGATGCTGAC CAAACAGAAC 
AATTCATTCC AGCGATCAAA AAGGAGATAC TTTAAGCTTC GAGGGCGAAC GCTTTACTAT 
GCCAAAACGG CAAAGTCAAT CATATTTGAT GAGGTGGATC TGACAGATGC CAGCGTAGCT 
GAATCCAGTA CCAAAAACGT CAACAACAGT TTTACGGTCA TAACTCCATG CAGGAAGCTC 
ATCTTGTGTG CTGATAACAG AAAAGAAATG GAAGATTGGA TTGCAGCATT AAAGACTGTG 
CAGAACAGGG AGCACTTTGA GCCCACCCAG TACAGCATGG ACCACTTCTC AGGGATGCAC 
AATTGGTACG CCTGTTCCCA CGCGAGGCCG ACCTACTGCA ATGTGTGCCG TGAGGCTCTG 
TCTGGGGTCA CGTCGCACGG GCTGTCCTGC GAGGTGTGCA AATTTAAGGC CCACAAGCGC 
TGTGCTGTGC GTGCAACCAA TAACTGCAAG TGGACCACAC TGGCCTCGAT CGGGAAGGAC 
ATCATTGAAG ATGCAGATGG GATTGCAATG CCCCACCAGT GGTTGGAAGG AAACCTACCT 
GTGAGCGCCA AGTGCACTGT GTGCGACAAG ACCTGTGGCA GTGTGCTGCG CCTGCAGGAC 
TGGCGCTGCC TCTGGTGCAA GGCCATGGTT CACACATCGT GTAAAGAATC CTTGCTGACC 
AAGTGCCCAC TTGGCCTGTG CAAAGTGTCA GTCATCCCAC CCACGGCTCT CAACAGCATC 
GACTCCGATG GGTTCTGGAA GGCCAGCTGT CCTCCTTCTT GCACAAGCCC ACTGTTGGTC 
TTCGTCAATT CAAAAAGTGG GGACAACCAG GGTGTGAAGT TCCTCAGAAG ATTCAAACAG 
CTACTAAACC CCGCCCAGGT CTTCGACCTC ATGAACGGAG GCCCACACCT CGGCTTACGG 
TTATTCCAGA AGTTTGACAC ATTCCGGATT CTGGTTTGTG GCGGGGATGG AAGTGTTGGC 
TGGGTCCTCT CCGAAATCGA CAGCCTCAAC CTTCATAAAC AGTGTCAGCT GGGAGTGCTG 
CCGCTCGGCA CAGGGAACGA CTTGGCCCGA GTACTGGGCT GGGGCTCAGC CTGCGATGAC 
GACACCCAGC TCCCCCAGAT CTTGGAGAAG TTGGAGAGAG CCAGCACCAA GATGCTGGAC 
AGGTGGAGCG TCATGGCATA CGAGGCCAAG CTCCCCCGGC AGGCCTCCTC CTCTACCGTC 
ACCGAAGACT TCAGCGAGGA TTCCGAGGTA CAGCAGATTC TCTTCTATGA AGACTCGGTT 
GCAGCCCACC TTTCTAAAAT CCTCACCTCG GACCAGCACT CGGTGGTCAT CTCCTCGGCC 
AAAGTGCTCT GTGAGACGGT GAAGGACTTC GTGGCACGGG TGGGGAAGGC CTATGAGAAG 
ACGACCGAGA GCTCGGAGGA GTCAGAGGTC ATGGCCAAGA AGTGCTCTGT CCTGAAAGAG 
AAGCTGGATT CCCTTCTCAA GACCTTGGAC GATGAGTCCC AGGCCTCGTC CTCTCTGCCC 
AACCCGCCCC CCACCATTGC CGAGGAGGCT GAAGATGGAG ATGGGTCGGG CAGCATCTGC 
GGTTCCACCG GAGACCGCTT GGTGGCATCA GCTTGCCCGG CCCGGCCGCA GATATTCCGG 
CCTCGAGAAC AGCTCATGCT GAGAGCCAAC AGCCTGAAGA AAGCAATTCG TCAGATCATA 
GAACACACAG AAAAAGCTGT CGATGAGCAG AATGCCCAGA CCCAGGAGCA GGAGGGCTTC 
GTCCTGGGCC TCTCTGAGTC AGAGGAGAAG ATGGACCACA GAGTGTGCCC ACCACTGTCC 
CACAGCGAGA GCTTCGGGGT CCCCAAGGGG AGGAGCCAGC GCAAAGTGTC GAAATCTCCG 
TGTGAAAAGC TGATCAGCAA AGGGAGTCTG TCCCTAGGCA GTTCTGCTTC CCTTCCGCCC 
CAGCCGGGAA GCCGGGACGG CCTGCCTGCG CTCAACACCA AGATCCTGTA CCCAAATGTC 
CGGGCTGGAA TGTCTGGTTC CTTACCCGGT GGCTCAGTCA TCAGTCGCCT GTTAATTAAT 
GCTGATCCCT TCAACTCTGA ACCAGAAACC CTAGAGTATT ACACGGAGAA ATGTGTCATG 
AACAACTATT TTGGCATTGG CCTGGATGCG AAGATATCCC TGGACTTTAA CAACAAGCGC 
GATGAGCACC CAGAGAAGTG CAGGAGCCGA ACCAAGAACA TGATGTGGTA TGGAGTTCTT 
GGAACCAAAG AGTTGCTGCA CAGAACCTAC AAGAACCTGG AGCAAAAGGT CTTGCTGGAG 
TGTGACGGGC GACCCATCCC ACTCCCCAGT CTTCAGGGAA TTGCTGTCCT TAACATTCCC 
AGCTATGCCG GAGGAACCAA CTTCTGGGGG GGTACCAAGG AAGATGATAC TTTCGCAGCT 
CCATCATTCG ATGACAAGAT TCTGGAGGTG GTCGCCGTGT TCGGCAGCAT GCAGATGGCC 
GTCTCTCGAG TCATCAGGCT ACAGCATCAT CGGATCGCCC AGTGTCGCAC GGTGAAGATC 
TCCATCCTTG GGGATGAGGG CGTGCCTGTG CAGGTGGACG GAGAGGCCTG GGTCCAGCCG 
CCAGGGTACA TTCGGATTGT CCACAAGAAC CGGGCACAGA CACTGACCAG AGACAGGGCA 
TTTGAGAGCA CCCTGAAGTC CTGGGAAGAC AAGCAGAAGT GCGAGCTGCC CCGCCCTCCA 
TCCTGTTCCC TGCACCCGGA GATGCTGTCC GAGGAGGAGG CCACCCAGAT GGACCAGTTT 
GGGCAGGCAG CAGGGGTCCT CATTCACAGT ATCCGAGAAA TAGCTCAGTC TCACCGGGAC 
ATGGAGCAGG AACTGGCCCA CGCCGTCAAT GCCAGCTCCA AGTCCATGGA CCGTGTGTAT 
GGCAAGCCCA GAACCACAGA GGGGCTCAAC TGCAGCTTCG TCCTGGAAAT GGTGAATAAC 
TTCAGAGCTC TGCGCAGTGA GACGGAGCTG CTGCTGTCTG GGAAGATGGC CCTGCAGCTG 
GATCCGCCTC AGAAGGAGCA GCTGGGGAGT GCTCTTGCCG AGATGGACCG ACAGCTCAGG 
AGGCTGGCAG ACACCCCGTG GCTCTGCCAG TCCGCAGAGC CCGGCGACGA AGAGAGTGTG 
ATGCTGGATC TTGCCAAGCG CAGTCGCAGT GGTAAATTCC GCCTCGTGAC CAAGTTTAAA 
AAGGAGAAAA ACAACAAGAA CAAAGAAGCT CACAGTAGCC TGGGAGCCCC GGTTCACCTC 
TGGGGGACAG AGGAGGTTGC TGCCTGGCTG GAGCACCTCA GTCTCTGTGA GTATAAGGAC 
ATCTTCACAC GGCACGACAT CCGGGGCTCT GAGCTCCTGC ACCTGGAGCG GAGGGACCTC 
AAGGACCTGG GCGTGACCAA GGTGGGCCAC ATGAAGAGGA TCCTGTGTGG CATCAAGGAG 
CTGAGCCGCA GCGCCCCCGC CGTCGAGGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( NM_003648.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003639.2 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14020
Clone ID Related Accession (Same CDS sequence)	NM_003648.3 , NM_003648.2
Accession Version	NM_003648.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D73409.1, D63479.3 and BC006561.2. On Nov 27, 2002 this sequence version replaced NM_003648.1. Transcript Variant: This variant (1) differs in the 5' UTR and uses a different in-frame start codon, compared to variant 2. The encoded isoform (1) has a shorter, distinct N-terminus, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D73409.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATATGT TTCTGTACTT TCAGACCATC ATCAAAGAGG GGATGCTGAC CAAACAGAAC 
AATTCATTCC AGCGATCAAA AAGGAGATAC TTTAAGCTTC GAGGGCGAAC GCTTTACTAT 
GCCAAAACGG CAAAGTCAAT CATATTTGAT GAGGTGGATC TGACAGATGC CAGCGTAGCT 
GAATCCAGTA CCAAAAACGT CAACAACAGT TTTACGGTCA TAACTCCATG CAGGAAGCTC 
ATCTTGTGTG CTGATAACAG AAAAGAAATG GAAGATTGGA TTGCAGCATT AAAGACTGTG 
CAGAACAGGG AGCACTTTGA GCCCACCCAG TACAGCATGG ACCACTTCTC AGGGATGCAC 
AATTGGTACG CCTGTTCCCA CGCGAGGCCG ACCTACTGCA ATGTGTGCCG TGAGGCTCTG 
TCTGGGGTCA CGTCGCACGG GCTGTCCTGC GAGGTGTGCA AATTTAAGGC CCACAAGCGC 
TGTGCTGTGC GTGCAACCAA TAACTGCAAG TGGACCACAC TGGCCTCGAT CGGGAAGGAC 
ATCATTGAAG ATGCAGATGG GATTGCAATG CCCCACCAGT GGTTGGAAGG AAACCTACCT 
GTGAGCGCCA AGTGCACTGT GTGCGACAAG ACCTGTGGCA GTGTGCTGCG CCTGCAGGAC 
TGGCGCTGCC TCTGGTGCAA GGCCATGGTT CACACATCGT GTAAAGAATC CTTGCTGACC 
AAGTGCCCAC TTGGCCTGTG CAAAGTGTCA GTCATCCCAC CCACGGCTCT CAACAGCATC 
GACTCCGATG GGTTCTGGAA GGCCAGCTGT CCTCCTTCTT GCACAAGCCC ACTGTTGGTC 
TTCGTCAATT CAAAAAGTGG GGACAACCAG GGTGTGAAGT TCCTCAGAAG ATTCAAACAG 
CTACTAAACC CCGCCCAGGT CTTCGACCTC ATGAACGGAG GCCCACACCT CGGCTTACGG 
TTATTCCAGA AGTTTGACAC ATTCCGGATT CTGGTTTGTG GCGGGGATGG AAGTGTTGGC 
TGGGTCCTCT CCGAAATCGA CAGCCTCAAC CTTCATAAAC AGTGTCAGCT GGGAGTGCTG 
CCGCTCGGCA CAGGGAACGA CTTGGCCCGA GTACTGGGCT GGGGCTCAGC CTGCGATGAC 
GACACCCAGC TCCCCCAGAT CTTGGAGAAG TTGGAGAGAG CCAGCACCAA GATGCTGGAC 
AGGTGGAGCG TCATGGCATA CGAGGCCAAG CTCCCCCGGC AGGCCTCCTC CTCTACCGTC 
ACCGAAGACT TCAGCGAGGA TTCCGAGGTA CAGCAGATTC TCTTCTATGA AGACTCGGTT 
GCAGCCCACC TTTCTAAAAT CCTCACCTCG GACCAGCACT CGGTGGTCAT CTCCTCGGCC 
AAAGTGCTCT GTGAGACGGT GAAGGACTTC GTGGCACGGG TGGGGAAGGC CTATGAGAAG 
ACGACCGAGA GCTCGGAGGA GTCAGAGGTC ATGGCCAAGA AGTGCTCTGT CCTGAAAGAG 
AAGCTGGATT CCCTTCTCAA GACCTTGGAC GATGAGTCCC AGGCCTCGTC CTCTCTGCCC 
AACCCGCCCC CCACCATTGC CGAGGAGGCT GAAGATGGAG ATGGGTCGGG CAGCATCTGC 
GGTTCCACCG GAGACCGCTT GGTGGCATCA GCTTGCCCGG CCCGGCCGCA GATATTCCGG 
CCTCGAGAAC AGCTCATGCT GAGAGCCAAC AGCCTGAAGA AAGCAATTCG TCAGATCATA 
GAACACACAG AAAAAGCTGT CGATGAGCAG AATGCCCAGA CCCAGGAGCA GGAGGGCTTC 
GTCCTGGGCC TCTCTGAGTC AGAGGAGAAG ATGGACCACA GAGTGTGCCC ACCACTGTCC 
CACAGCGAGA GCTTCGGGGT CCCCAAGGGG AGGAGCCAGC GCAAAGTGTC GAAATCTCCG 
TGTGAAAAGC TGATCAGCAA AGGGAGTCTG TCCCTAGGCA GTTCTGCTTC CCTTCCGCCC 
CAGCCGGGAA GCCGGGACGG CCTGCCTGCG CTCAACACCA AGATCCTGTA CCCAAATGTC 
CGGGCTGGAA TGTCTGGTTC CTTACCCGGT GGCTCAGTCA TCAGTCGCCT GTTAATTAAT 
GCTGATCCCT TCAACTCTGA ACCAGAAACC CTAGAGTATT ACACGGAGAA ATGTGTCATG 
AACAACTATT TTGGCATTGG CCTGGATGCG AAGATATCCC TGGACTTTAA CAACAAGCGC 
GATGAGCACC CAGAGAAGTG CAGGAGCCGA ACCAAGAACA TGATGTGGTA TGGAGTTCTT 
GGAACCAAAG AGTTGCTGCA CAGAACCTAC AAGAACCTGG AGCAAAAGGT CTTGCTGGAG 
TGTGACGGGC GACCCATCCC ACTCCCCAGT CTTCAGGGAA TTGCTGTCCT TAACATTCCC 
AGCTATGCCG GAGGAACCAA CTTCTGGGGG GGTACCAAGG AAGATGATAC TTTCGCAGCT 
CCATCATTCG ATGACAAGAT TCTGGAGGTG GTCGCCGTGT TCGGCAGCAT GCAGATGGCC 
GTCTCTCGAG TCATCAGGCT ACAGCATCAT CGGATCGCCC AGTGTCGCAC GGTGAAGATC 
TCCATCCTTG GGGATGAGGG CGTGCCTGTG CAGGTGGACG GAGAGGCCTG GGTCCAGCCG 
CCAGGGTACA TTCGGATTGT CCACAAGAAC CGGGCACAGA CACTGACCAG AGACAGGGCA 
TTTGAGAGCA CCCTGAAGTC CTGGGAAGAC AAGCAGAAGT GCGAGCTGCC CCGCCCTCCA 
TCCTGTTCCC TGCACCCGGA GATGCTGTCC GAGGAGGAGG CCACCCAGAT GGACCAGTTT 
GGGCAGGCAG CAGGGGTCCT CATTCACAGT ATCCGAGAAA TAGCTCAGTC TCACCGGGAC 
ATGGAGCAGG AACTGGCCCA CGCCGTCAAT GCCAGCTCCA AGTCCATGGA CCGTGTGTAT 
GGCAAGCCCA GAACCACAGA GGGGCTCAAC TGCAGCTTCG TCCTGGAAAT GGTGAATAAC 
TTCAGAGCTC TGCGCAGTGA GACGGAGCTG CTGCTGTCTG GGAAGATGGC CCTGCAGCTG 
GATCCGCCTC AGAAGGAGCA GCTGGGGAGT GCTCTTGCCG AGATGGACCG ACAGCTCAGG 
AGGCTGGCAG ACACCCCGTG GCTCTGCCAG TCCGCAGAGC CCGGCGACGA AGAGAGTGTG 
ATGCTGGATC TTGCCAAGCG CAGTCGCAGT GGTAAATTCC GCCTCGTGAC CAAGTTTAAA 
AAGGAGAAAA ACAACAAGAA CAAAGAAGCT CACAGTAGCC TGGGAGCCCC GGTTCACCTC 
TGGGGGACAG AGGAGGTTGC TGCCTGGCTG GAGCACCTCA GTCTCTGTGA GTATAAGGAC 
ATCTTCACAC GGCACGACAT CCGGGGCTCT GAGCTCCTGC ACCTGGAGCG GAGGGACCTC 
AAGGACCTGG GCGTGACCAA GGTGGGCCAC ATGAAGAGGA TCCTGTGTGG CATCAAGGAG 
CTGAGCCGCA GCGCCCCCGC CGTCGAGGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003639.2
CDS	80..3592
Translation	MNMFLYFQTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	NM_003648.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003648.2

ORF Insert Sequence:

ATGAATATGT TTCTGTACTT TCAGACCATC ATCAAAGAGG GGATGCTGAC CAAACAGAAC 
AATTCATTCC AGCGATCAAA AAGGAGATAC TTTAAGCTTC GAGGGCGAAC GCTTTACTAT 
GCCAAAACGG CAAAGTCAAT CATATTTGAT GAGGTGGATC TGACAGATGC CAGCGTAGCT 
GAATCCAGTA CCAAAAACGT CAACAACAGT TTTACGGTCA TAACTCCATG CAGGAAGCTC 
ATCTTGTGTG CTGATAACAG AAAAGAAATG GAAGATTGGA TTGCAGCATT AAAGACTGTG 
CAGAACAGGG AGCACTTTGA GCCCACCCAG TACAGCATGG ACCACTTCTC AGGGATGCAC 
AATTGGTACG CCTGTTCCCA CGCGAGGCCG ACCTACTGCA ATGTGTGCCG TGAGGCTCTG 
TCTGGGGTCA CGTCGCACGG GCTGTCCTGC GAGGTGTGCA AATTTAAGGC CCACAAGCGC 
TGTGCTGTGC GTGCAACCAA TAACTGCAAG TGGACCACAC TGGCCTCGAT CGGGAAGGAC 
ATCATTGAAG ATGCAGATGG GATTGCAATG CCCCACCAGT GGTTGGAAGG AAACCTACCT 
GTGAGCGCCA AGTGCACTGT GTGCGACAAG ACCTGTGGCA GTGTGCTGCG CCTGCAGGAC 
TGGCGCTGCC TCTGGTGCAA GGCCATGGTT CACACATCGT GTAAAGAATC CTTGCTGACC 
AAGTGCCCAC TTGGCCTGTG CAAAGTGTCA GTCATCCCAC CCACGGCTCT CAACAGCATC 
GACTCCGATG GGTTCTGGAA GGCCAGCTGT CCTCCTTCTT GCACAAGCCC ACTGTTGGTC 
TTCGTCAATT CAAAAAGTGG GGACAACCAG GGTGTGAAGT TCCTCAGAAG ATTCAAACAG 
CTACTAAACC CCGCCCAGGT CTTCGACCTC ATGAACGGAG GCCCACACCT CGGCTTACGG 
TTATTCCAGA AGTTTGACAC ATTCCGGATT CTGGTTTGTG GCGGGGATGG AAGTGTTGGC 
TGGGTCCTCT CCGAAATCGA CAGCCTCAAC CTTCATAAAC AGTGTCAGCT GGGAGTGCTG 
CCGCTCGGCA CAGGGAACGA CTTGGCCCGA GTACTGGGCT GGGGCTCAGC CTGCGATGAC 
GACACCCAGC TCCCCCAGAT CTTGGAGAAG TTGGAGAGAG CCAGCACCAA GATGCTGGAC 
AGGTGGAGCG TCATGGCATA CGAGGCCAAG CTCCCCCGGC AGGCCTCCTC CTCTACCGTC 
ACCGAAGACT TCAGCGAGGA TTCCGAGGTA CAGCAGATTC TCTTCTATGA AGACTCGGTT 
GCAGCCCACC TTTCTAAAAT CCTCACCTCG GACCAGCACT CGGTGGTCAT CTCCTCGGCC 
AAAGTGCTCT GTGAGACGGT GAAGGACTTC GTGGCACGGG TGGGGAAGGC CTATGAGAAG 
ACGACCGAGA GCTCGGAGGA GTCAGAGGTC ATGGCCAAGA AGTGCTCTGT CCTGAAAGAG 
AAGCTGGATT CCCTTCTCAA GACCTTGGAC GATGAGTCCC AGGCCTCGTC CTCTCTGCCC 
AACCCGCCCC CCACCATTGC CGAGGAGGCT GAAGATGGAG ATGGGTCGGG CAGCATCTGC 
GGTTCCACCG GAGACCGCTT GGTGGCATCA GCTTGCCCGG CCCGGCCGCA GATATTCCGG 
CCTCGAGAAC AGCTCATGCT GAGAGCCAAC AGCCTGAAGA AAGCAATTCG TCAGATCATA 
GAACACACAG AAAAAGCTGT CGATGAGCAG AATGCCCAGA CCCAGGAGCA GGAGGGCTTC 
GTCCTGGGCC TCTCTGAGTC AGAGGAGAAG ATGGACCACA GAGTGTGCCC ACCACTGTCC 
CACAGCGAGA GCTTCGGGGT CCCCAAGGGG AGGAGCCAGC GCAAAGTGTC GAAATCTCCG 
TGTGAAAAGC TGATCAGCAA AGGGAGTCTG TCCCTAGGCA GTTCTGCTTC CCTTCCGCCC 
CAGCCGGGAA GCCGGGACGG CCTGCCTGCG CTCAACACCA AGATCCTGTA CCCAAATGTC 
CGGGCTGGAA TGTCTGGTTC CTTACCCGGT GGCTCAGTCA TCAGTCGCCT GTTAATTAAT 
GCTGATCCCT TCAACTCTGA ACCAGAAACC CTAGAGTATT ACACGGAGAA ATGTGTCATG 
AACAACTATT TTGGCATTGG CCTGGATGCG AAGATATCCC TGGACTTTAA CAACAAGCGC 
GATGAGCACC CAGAGAAGTG CAGGAGCCGA ACCAAGAACA TGATGTGGTA TGGAGTTCTT 
GGAACCAAAG AGTTGCTGCA CAGAACCTAC AAGAACCTGG AGCAAAAGGT CTTGCTGGAG 
TGTGACGGGC GACCCATCCC ACTCCCCAGT CTTCAGGGAA TTGCTGTCCT TAACATTCCC 
AGCTATGCCG GAGGAACCAA CTTCTGGGGG GGTACCAAGG AAGATGATAC TTTCGCAGCT 
CCATCATTCG ATGACAAGAT TCTGGAGGTG GTCGCCGTGT TCGGCAGCAT GCAGATGGCC 
GTCTCTCGAG TCATCAGGCT ACAGCATCAT CGGATCGCCC AGTGTCGCAC GGTGAAGATC 
TCCATCCTTG GGGATGAGGG CGTGCCTGTG CAGGTGGACG GAGAGGCCTG GGTCCAGCCG 
CCAGGGTACA TTCGGATTGT CCACAAGAAC CGGGCACAGA CACTGACCAG AGACAGGGCA 
TTTGAGAGCA CCCTGAAGTC CTGGGAAGAC AAGCAGAAGT GCGAGCTGCC CCGCCCTCCA 
TCCTGTTCCC TGCACCCGGA GATGCTGTCC GAGGAGGAGG CCACCCAGAT GGACCAGTTT 
GGGCAGGCAG CAGGGGTCCT CATTCACAGT ATCCGAGAAA TAGCTCAGTC TCACCGGGAC 
ATGGAGCAGG AACTGGCCCA CGCCGTCAAT GCCAGCTCCA AGTCCATGGA CCGTGTGTAT 
GGCAAGCCCA GAACCACAGA GGGGCTCAAC TGCAGCTTCG TCCTGGAAAT GGTGAATAAC 
TTCAGAGCTC TGCGCAGTGA GACGGAGCTG CTGCTGTCTG GGAAGATGGC CCTGCAGCTG 
GATCCGCCTC AGAAGGAGCA GCTGGGGAGT GCTCTTGCCG AGATGGACCG ACAGCTCAGG 
AGGCTGGCAG ACACCCCGTG GCTCTGCCAG TCCGCAGAGC CCGGCGACGA AGAGAGTGTG 
ATGCTGGATC TTGCCAAGCG CAGTCGCAGT GGTAAATTCC GCCTCGTGAC CAAGTTTAAA 
AAGGAGAAAA ACAACAAGAA CAAAGAAGCT CACAGTAGCC TGGGAGCCCC GGTTCACCTC 
TGGGGGACAG AGGAGGTTGC TGCCTGGCTG GAGCACCTCA GTCTCTGTGA GTATAAGGAC 
ATCTTCACAC GGCACGACAT CCGGGGCTCT GAGCTCCTGC ACCTGGAGCG GAGGGACCTC 
AAGGACCTGG GCGTGACCAA GGTGGGCCAC ATGAAGAGGA TCCTGTGTGG CATCAAGGAG 
CTGAGCCGCA GCGCCCCCGC CGTCGAGGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_005246107.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246164.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X9, mRNA.

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CloneID	OHu36702
Clone ID Related Accession (Same CDS sequence)	XM_005246107.3
Accession Version	XM_005246107.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005246107.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGTA AGGTCATAAC TCCATGCAGG AAGCTCATCT TGTGTGCTGA TAACAGAAAA 
GAAATGGAAG ATTGGATTGC AGCATTAAAG ACTGTGCAGA ACAGGGAGCA CTTTGAGCCC 
ACCCAGTACA GCATGGACCA CTTCTCAGGG ATGCACAATT GGTACGCCTG TTCCCACGCG 
AGGCCGACCT ACTGCAATGT GTGCCGTGAG GCTCTGTCTG GGGTCACGTC GCACGGGCTG 
TCCTGCGAGG TGTGCAAATT TAAGGCCCAC AAGCGCTGTG CTGTGCGTGC AACCAATAAC 
TGCAAGTGGA CCACACTGGC CTCGATCGGG AAGGACATCA TTGAAGATGC AGATGGGATT 
GCAATGCCCC ACCAGTGGTT GGAAGGAAAC CTACCTGTGA GCGCCAAGTG CACTGTGTGC 
GACAAGACCT GTGGCAGTGT GCTGCGCCTG CAGGACTGGC GCTGCCTCTG GTGCAAGGCC 
ATGGTTCACA CATCGTGTAA AGAATCCTTG CTGACCAAGT GCCCACTTGG CCTGTGCAAA 
GTGTCAGTCA TCCCACCCAC GGCTCTCAAC AGCATCGACT CCGATGGGTT CTGGAAGGCC 
AGCTGTCCTC CTTCTTGCAC AAGCCCACTG TTGGTCTTCG TCAATTCAAA AAGTGGGGAC 
AACCAGGGTG TGAAGTTCCT CAGAAGATTC AAACAGCTAC TAAACCCCGC CCAGGTCTTC 
GACCTCATGA ACGGAGGCCC ACACCTCGGC TTACGGTTAT TCCAGAAGTT TGACACATTC 
CGGATTCTGG TTTGTGGCGG GGATGGAAGT GTTGGCTGGG TCCTCTCCGA AATCGACAGC 
CTCAACCTTC ATAAACAGTG TCAGCTGGGA GTGCTGCCGC TCGGCACAGG GAACGACTTG 
GCCCGAGTAC TGGGCTGGGG CTCAGCCTGC GATGACGACA CCCAGCTCCC CCAGATCTTG 
GAGAAGTTGG AGAGAGCCAG CACCAAGATG CTGGACAGGT GGAGCGTCAT GGCATACGAG 
GCCAAGCTCC CCCGGCAGGC CTCCTCCTCT ACCGTCACCG AAGACTTCAG CGAGGATTCC 
GAGGTACAGC AGATTCTCTT CTATGAAGAC TCGGTTGCAG CCCACCTTTC TAAAATCCTC 
ACCTCGGACC AGCACTCGGT GGTCATCTCC TCGGCCAAAG TGCTCTGTGA GACGGTGAAG 
GACTTCGTGG CACGGGTGGG GAAGGCCTAT GAGAAGACGA CCGAGAGCTC GGAGGAGTCA 
GAGGTCATGG CCAAGAAGTG CTCTGTCCTG AAAGAGAAGC TGGATTCCCT TCTCAAGACC 
TTGGACGATG AGTCCCAGGC CTCGTCCTCT CTGCCCAACC CGCCCCCCAC CATTGCCGAG 
GAGGCTGAAG ATGGAGATGG GTCGGGCAGC ATCTGCGGTT CCACCGGAGA CCGCTTGGTG 
GCATCAGCTT GCCCGGCCCG GCCGCAGATA TTCCGGCCTC GAGAACAGCT CATGCTGAGA 
GCCAACAGCC TGAAGAAAGC AATTCGTCAG ATCATAGAAC ACACAGAAAA AGCTGTCGAT 
GAGCAGAATG CCCAGACCCA GGAGCAGGAG GGCTTCGTCC TGGGCCTCTC TGAGTCAGAG 
GAGAAGATGG ACCACAGAGT GTGCCCACCA CTGTCCCACA GCGAGAGCTT CGGGGTCCCC 
AAGGGGAGGA GCCAGCGCAA AGTGTCGAAA TCTCCGTGTG AAAAGCTGAT CAGCAAAGGG 
AGTCTGTCCC TAGGCAGTTC TGCTTCCCTT CCGCCCCAGC CGGGAAGCCG GGACGGCCTG 
CCTGCGCTCA ACACCAAGAT CCTGTACCCA AATGTCCGGG CTGGAATGTC TGGTTCCTTA 
CCCGGTGGCT CAGTCATCAG TCGCCTGTTA ATTAATGCTG ATCCCTTCAA CTCTGAACCA 
GAAACCCTAG AGTATTACAC GGAGAAATGT GTCATGAACA ACTATTTTGG CATTGGCCTG 
GATGCGAAGA TATCCCTGGA CTTTAACAAC AAGCGCGATG AGCACCCAGA GAAGTGCAGG 
AGCCGAACCA AGAACATGAT GTGGTATGGA GTTCTTGGAA CCAAAGAGTT GCTGCACAGA 
ACCTACAAGA ACCTGGAGCA AAAGGTCTTG CTGGAGTGTG ACGGGCGACC CATCCCACTC 
CCCAGTCTTC AGGGAATTGC TGTCCTTAAC ATTCCCAGCT ATGCCGGAGG AACCAACTTC 
TGGGGGGGTA CCAAGGAAGA TGATACTTTC GCAGCTCCAT CATTCGATGA CAAGATTCTG 
GAGGTGGTCG CCGTGTTCGG CAGCATGCAG ATGGCCGTCT CTCGAGTCAT CAGGCTACAG 
CATCATCGGA TCGCCCAGTG TCGCACGGTG AAGATCTCCA TCCTTGGGGA TGAGGGCGTG 
CCTGTGCAGG TGGACGGAGA GGCCTGGGTC CAGCCGCCAG GGTACATTCG GATTGTCCAC 
AAGAACCGGG CACAGACACT GACCAGAGAC AGGGCATTTG AGAGCACCCT GAAGTCCTGG 
GAAGACAAGC AGAAGTGCGA GCTGCCCCGC CCTCCATCCT GTTCCCTGCA CCCGGAGATG 
CTGTCCGAGG AGGAGGCCAC CCAGATGGAC CAGTTTGGGC AGGCAGCAGG GGTCCTCATT 
CACAGTATCC GAGAAATAGC TCAGTCTCAC CGGGACATGG AGCAGGAACT GGCCCACGCC 
GTCAATGCCA GCTCCAAGTC CATGGACCGT GTGTATGGCA AGCCCAGAAC CACAGAGGGG 
CTCAACTGCA GCTTCGTCCT GGAAATGGTG AATAACTTCA GAGCTCTGCG CAGTGAGACG 
GAGCTGCTGC TGTCTGGGAA GATGGCCCTG CAGCTGGATC CGCCTCAGAA GGAGCAGCTG 
GGGAGTGCTC TTGCCGAGAT GGACCGACAG CTCAGGAGGC TGGCAGACAC CCCGTGGCTC 
TGCCAGTCCG CAGAGCCCGG CGACGAAGAG AGTGTGATGC TGGATCTTGC CAAGCGCAGT 
CGCAGTGGTA AATTCCGCCT CGTGACCAAG TTTAAAAAGG AGAAAAACAA CAAGAACAAA 
GAAGCTCACA GTAGCCTGGG AGCCCCGGTT CACCTCTGGG GGACAGAGGA GGTTGCTGCC 
TGGCTGGAGC ACCTCAGTCT CTGTGAGTAT AAGGACATCT TCACACGGCA CGACATCCGG 
GGCTCTGAGC TCCTGCACCT GGAGCGGAGG GACCTCAAGG ACCTGGGCGT GACCAAGGTG 
GGCCACATGA AGAGGATCCT GTGTGGCATC AAGGAGCTGA GCCGCAGCGC CCCCGCCGTC 
GAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246164.1
CDS	16..3324
Translation	MHSKVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_005246107.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246107.3

ORF Insert Sequence:

ATGCACAGTA AGGTCATAAC TCCATGCAGG AAGCTCATCT TGTGTGCTGA TAACAGAAAA 
GAAATGGAAG ATTGGATTGC AGCATTAAAG ACTGTGCAGA ACAGGGAGCA CTTTGAGCCC 
ACCCAGTACA GCATGGACCA CTTCTCAGGG ATGCACAATT GGTACGCCTG TTCCCACGCG 
AGGCCGACCT ACTGCAATGT GTGCCGTGAG GCTCTGTCTG GGGTCACGTC GCACGGGCTG 
TCCTGCGAGG TGTGCAAATT TAAGGCCCAC AAGCGCTGTG CTGTGCGTGC AACCAATAAC 
TGCAAGTGGA CCACACTGGC CTCGATCGGG AAGGACATCA TTGAAGATGC AGATGGGATT 
GCAATGCCCC ACCAGTGGTT GGAAGGAAAC CTACCTGTGA GCGCCAAGTG CACTGTGTGC 
GACAAGACCT GTGGCAGTGT GCTGCGCCTG CAGGACTGGC GCTGCCTCTG GTGCAAGGCC 
ATGGTTCACA CATCGTGTAA AGAATCCTTG CTGACCAAGT GCCCACTTGG CCTGTGCAAA 
GTGTCAGTCA TCCCACCCAC GGCTCTCAAC AGCATCGACT CCGATGGGTT CTGGAAGGCC 
AGCTGTCCTC CTTCTTGCAC AAGCCCACTG TTGGTCTTCG TCAATTCAAA AAGTGGGGAC 
AACCAGGGTG TGAAGTTCCT CAGAAGATTC AAACAGCTAC TAAACCCCGC CCAGGTCTTC 
GACCTCATGA ACGGAGGCCC ACACCTCGGC TTACGGTTAT TCCAGAAGTT TGACACATTC 
CGGATTCTGG TTTGTGGCGG GGATGGAAGT GTTGGCTGGG TCCTCTCCGA AATCGACAGC 
CTCAACCTTC ATAAACAGTG TCAGCTGGGA GTGCTGCCGC TCGGCACAGG GAACGACTTG 
GCCCGAGTAC TGGGCTGGGG CTCAGCCTGC GATGACGACA CCCAGCTCCC CCAGATCTTG 
GAGAAGTTGG AGAGAGCCAG CACCAAGATG CTGGACAGGT GGAGCGTCAT GGCATACGAG 
GCCAAGCTCC CCCGGCAGGC CTCCTCCTCT ACCGTCACCG AAGACTTCAG CGAGGATTCC 
GAGGTACAGC AGATTCTCTT CTATGAAGAC TCGGTTGCAG CCCACCTTTC TAAAATCCTC 
ACCTCGGACC AGCACTCGGT GGTCATCTCC TCGGCCAAAG TGCTCTGTGA GACGGTGAAG 
GACTTCGTGG CACGGGTGGG GAAGGCCTAT GAGAAGACGA CCGAGAGCTC GGAGGAGTCA 
GAGGTCATGG CCAAGAAGTG CTCTGTCCTG AAAGAGAAGC TGGATTCCCT TCTCAAGACC 
TTGGACGATG AGTCCCAGGC CTCGTCCTCT CTGCCCAACC CGCCCCCCAC CATTGCCGAG 
GAGGCTGAAG ATGGAGATGG GTCGGGCAGC ATCTGCGGTT CCACCGGAGA CCGCTTGGTG 
GCATCAGCTT GCCCGGCCCG GCCGCAGATA TTCCGGCCTC GAGAACAGCT CATGCTGAGA 
GCCAACAGCC TGAAGAAAGC AATTCGTCAG ATCATAGAAC ACACAGAAAA AGCTGTCGAT 
GAGCAGAATG CCCAGACCCA GGAGCAGGAG GGCTTCGTCC TGGGCCTCTC TGAGTCAGAG 
GAGAAGATGG ACCACAGAGT GTGCCCACCA CTGTCCCACA GCGAGAGCTT CGGGGTCCCC 
AAGGGGAGGA GCCAGCGCAA AGTGTCGAAA TCTCCGTGTG AAAAGCTGAT CAGCAAAGGG 
AGTCTGTCCC TAGGCAGTTC TGCTTCCCTT CCGCCCCAGC CGGGAAGCCG GGACGGCCTG 
CCTGCGCTCA ACACCAAGAT CCTGTACCCA AATGTCCGGG CTGGAATGTC TGGTTCCTTA 
CCCGGTGGCT CAGTCATCAG TCGCCTGTTA ATTAATGCTG ATCCCTTCAA CTCTGAACCA 
GAAACCCTAG AGTATTACAC GGAGAAATGT GTCATGAACA ACTATTTTGG CATTGGCCTG 
GATGCGAAGA TATCCCTGGA CTTTAACAAC AAGCGCGATG AGCACCCAGA GAAGTGCAGG 
AGCCGAACCA AGAACATGAT GTGGTATGGA GTTCTTGGAA CCAAAGAGTT GCTGCACAGA 
ACCTACAAGA ACCTGGAGCA AAAGGTCTTG CTGGAGTGTG ACGGGCGACC CATCCCACTC 
CCCAGTCTTC AGGGAATTGC TGTCCTTAAC ATTCCCAGCT ATGCCGGAGG AACCAACTTC 
TGGGGGGGTA CCAAGGAAGA TGATACTTTC GCAGCTCCAT CATTCGATGA CAAGATTCTG 
GAGGTGGTCG CCGTGTTCGG CAGCATGCAG ATGGCCGTCT CTCGAGTCAT CAGGCTACAG 
CATCATCGGA TCGCCCAGTG TCGCACGGTG AAGATCTCCA TCCTTGGGGA TGAGGGCGTG 
CCTGTGCAGG TGGACGGAGA GGCCTGGGTC CAGCCGCCAG GGTACATTCG GATTGTCCAC 
AAGAACCGGG CACAGACACT GACCAGAGAC AGGGCATTTG AGAGCACCCT GAAGTCCTGG 
GAAGACAAGC AGAAGTGCGA GCTGCCCCGC CCTCCATCCT GTTCCCTGCA CCCGGAGATG 
CTGTCCGAGG AGGAGGCCAC CCAGATGGAC CAGTTTGGGC AGGCAGCAGG GGTCCTCATT 
CACAGTATCC GAGAAATAGC TCAGTCTCAC CGGGACATGG AGCAGGAACT GGCCCACGCC 
GTCAATGCCA GCTCCAAGTC CATGGACCGT GTGTATGGCA AGCCCAGAAC CACAGAGGGG 
CTCAACTGCA GCTTCGTCCT GGAAATGGTG AATAACTTCA GAGCTCTGCG CAGTGAGACG 
GAGCTGCTGC TGTCTGGGAA GATGGCCCTG CAGCTGGATC CGCCTCAGAA GGAGCAGCTG 
GGGAGTGCTC TTGCCGAGAT GGACCGACAG CTCAGGAGGC TGGCAGACAC CCCGTGGCTC 
TGCCAGTCCG CAGAGCCCGG CGACGAAGAG AGTGTGATGC TGGATCTTGC CAAGCGCAGT 
CGCAGTGGTA AATTCCGCCT CGTGACCAAG TTTAAAAAGG AGAAAAACAA CAAGAACAAA 
GAAGCTCACA GTAGCCTGGG AGCCCCGGTT CACCTCTGGG GGACAGAGGA GGTTGCTGCC 
TGGCTGGAGC ACCTCAGTCT CTGTGAGTAT AAGGACATCT TCACACGGCA CGACATCCGG 
GGCTCTGAGC TCCTGCACCT GGAGCGGAGG GACCTCAAGG ACCTGGGCGT GACCAAGGTG 
GGCCACATGA AGAGGATCCT GTGTGGCATC AAGGAGCTGA GCCGCAGCGC CCCCGCCGTC 
GAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512035.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510337.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X12, mRNA.

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CloneID	OHu36703
Clone ID Related Accession (Same CDS sequence)	NM_001377259.1 , XM_011512035.1 , XM_017005140.1 , XM_011512040.2 , XM_011512037.2 , XM_005246108.1
Accession Version	XM_011512035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATT GGATTGCAGC ATTAAAGACT GTGCAGAACA GGGAGCACTT TGAGCCCACC 
CAGTACAGCA TGGACCACTT CTCAGGGATG CACAATTGGT ACGCCTGTTC CCACGCGAGG 
CCGACCTACT GCAATGTGTG CCGTGAGGCT CTGTCTGGGG TCACGTCGCA CGGGCTGTCC 
TGCGAGGTGT GCAAATTTAA GGCCCACAAG CGCTGTGCTG TGCGTGCAAC CAATAACTGC 
AAGTGGACCA CACTGGCCTC GATCGGGAAG GACATCATTG AAGATGCAGA TGGGATTGCA 
ATGCCCCACC AGTGGTTGGA AGGAAACCTA CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC 
AAGACCTGTG GCAGTGTGCT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CGTGTAAAGA ATCCTTGCTG ACCAAGTGCC CACTTGGCCT GTGCAAAGTG 
TCAGTCATCC CACCCACGGC TCTCAACAGC ATCGACTCCG ATGGGTTCTG GAAGGCCAGC 
TGTCCTCCTT CTTGCACAAG CCCACTGTTG GTCTTCGTCA ATTCAAAAAG TGGGGACAAC 
CAGGGTGTGA AGTTCCTCAG AAGATTCAAA CAGCTACTAA ACCCCGCCCA GGTCTTCGAC 
CTCATGAACG GAGGCCCACA CCTCGGCTTA CGGTTATTCC AGAAGTTTGA CACATTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT GGCTGGGTCC TCTCCGAAAT CGACAGCCTC 
AACCTTCATA AACAGTGTCA GCTGGGAGTG CTGCCGCTCG GCACAGGGAA CGACTTGGCC 
CGAGTACTGG GCTGGGGCTC AGCCTGCGAT GACGACACCC AGCTCCCCCA GATCTTGGAG 
AAGTTGGAGA GAGCCAGCAC CAAGATGCTG GACAGGTGGA GCGTCATGGC ATACGAGGCC 
AAGCTCCCCC GGCAGGCCTC CTCCTCTACC GTCACCGAAG ACTTCAGCGA GGATTCCGAG 
GTACAGCAGA TTCTCTTCTA TGAAGACTCG GTTGCAGCCC ACCTTTCTAA AATCCTCACC 
TCGGACCAGC ACTCGGTGGT CATCTCCTCG GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC 
TTCGTGGCAC GGGTGGGGAA GGCCTATGAG AAGACGACCG AGAGCTCGGA GGAGTCAGAG 
GTCATGGCCA AGAAGTGCTC TGTCCTGAAA GAGAAGCTGG ATTCCCTTCT CAAGACCTTG 
GACGATGAGT CCCAGGCCTC GTCCTCTCTG CCCAACCCGC CCCCCACCAT TGCCGAGGAG 
GCTGAAGATG GAGATGGGTC GGGCAGCATC TGCGGTTCCA CCGGAGACCG CTTGGTGGCA 
TCAGCTTGCC CGGCCCGGCC GCAGATATTC CGGCCTCGAG AACAGCTCAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGTCAGATC ATAGAACACA CAGAAAAAGC TGTCGATGAG 
CAGAATGCCC AGACCCAGGA GCAGGAGGGC TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG 
AAGATGGACC ACAGAGTGTG CCCACCACTG TCCCACAGCG AGAGCTTCGG GGTCCCCAAG 
GGGAGGAGCC AGCGCAAAGT GTCGAAATCT CCGTGTGAAA AGCTGATCAG CAAAGGGAGT 
CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT 
GCGCTCAACA CCAAGATCCT GTACCCAAAT GTCCGGGCTG GAATGTCTGG TTCCTTACCC 
GGTGGCTCAG TCATCAGTCG CCTGTTAATT AATGCTGATC CCTTCAACTC TGAACCAGAA 
ACCCTAGAGT ATTACACGGA GAAATGTGTC ATGAACAACT ATTTTGGCAT TGGCCTGGAT 
GCGAAGATAT CCCTGGACTT TAACAACAAG CGCGATGAGC ACCCAGAGAA GTGCAGGAGC 
CGAACCAAGA ACATGATGTG GTATGGAGTT CTTGGAACCA AAGAGTTGCT GCACAGAACC 
TACAAGAACC TGGAGCAAAA GGTCTTGCTG GAGTGTGACG GGCGACCCAT CCCACTCCCC 
AGTCTTCAGG GAATTGCTGT CCTTAACATT CCCAGCTATG CCGGAGGAAC CAACTTCTGG 
GGGGGTACCA AGGAAGATGA TACTTTCGCA GCTCCATCAT TCGATGACAA GATTCTGGAG 
GTGGTCGCCG TGTTCGGCAG CATGCAGATG GCCGTCTCTC GAGTCATCAG GCTACAGCAT 
CATCGGATCG CCCAGTGTCG CACGGTGAAG ATCTCCATCC TTGGGGATGA GGGCGTGCCT 
GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG CCGCCAGGGT ACATTCGGAT TGTCCACAAG 
AACCGGGCAC AGACACTGAC CAGAGACAGG GCATTTGAGA GCACCCTGAA GTCCTGGGAA 
GACAAGCAGA AGTGCGAGCT GCCCCGCCCT CCATCCTGTT CCCTGCACCC GGAGATGCTG 
TCCGAGGAGG AGGCCACCCA GATGGACCAG TTTGGGCAGG CAGCAGGGGT CCTCATTCAC 
AGTATCCGAG AAATAGCTCA GTCTCACCGG GACATGGAGC AGGAACTGGC CCACGCCGTC 
AATGCCAGCT CCAAGTCCAT GGACCGTGTG TATGGCAAGC CCAGAACCAC AGAGGGGCTC 
AACTGCAGCT TCGTCCTGGA AATGGTGAAT AACTTCAGAG CTCTGCGCAG TGAGACGGAG 
CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG CTGGATCCGC CTCAGAAGGA GCAGCTGGGG 
AGTGCTCTTG CCGAGATGGA CCGACAGCTC AGGAGGCTGG CAGACACCCC GTGGCTCTGC 
CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT GTGATGCTGG ATCTTGCCAA GCGCAGTCGC 
AGTGGTAAAT TCCGCCTCGT GACCAAGTTT AAAAAGGAGA AAAACAACAA GAACAAAGAA 
GCTCACAGTA GCCTGGGAGC CCCGGTTCAC CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG 
CTGGAGCACC TCAGTCTCTG TGAGTATAAG GACATCTTCA CACGGCACGA CATCCGGGGC 
TCTGAGCTCC TGCACCTGGA GCGGAGGGAC CTCAAGGACC TGGGCGTGAC CAAGGTGGGC 
CACATGAAGA GGATCCTGTG TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510337.1
CDS	213..3458
Translation	MEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_011512035.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512035.1

ORF Insert Sequence:

ATGGAAGATT GGATTGCAGC ATTAAAGACT GTGCAGAACA GGGAGCACTT TGAGCCCACC 
CAGTACAGCA TGGACCACTT CTCAGGGATG CACAATTGGT ACGCCTGTTC CCACGCGAGG 
CCGACCTACT GCAATGTGTG CCGTGAGGCT CTGTCTGGGG TCACGTCGCA CGGGCTGTCC 
TGCGAGGTGT GCAAATTTAA GGCCCACAAG CGCTGTGCTG TGCGTGCAAC CAATAACTGC 
AAGTGGACCA CACTGGCCTC GATCGGGAAG GACATCATTG AAGATGCAGA TGGGATTGCA 
ATGCCCCACC AGTGGTTGGA AGGAAACCTA CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC 
AAGACCTGTG GCAGTGTGCT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CGTGTAAAGA ATCCTTGCTG ACCAAGTGCC CACTTGGCCT GTGCAAAGTG 
TCAGTCATCC CACCCACGGC TCTCAACAGC ATCGACTCCG ATGGGTTCTG GAAGGCCAGC 
TGTCCTCCTT CTTGCACAAG CCCACTGTTG GTCTTCGTCA ATTCAAAAAG TGGGGACAAC 
CAGGGTGTGA AGTTCCTCAG AAGATTCAAA CAGCTACTAA ACCCCGCCCA GGTCTTCGAC 
CTCATGAACG GAGGCCCACA CCTCGGCTTA CGGTTATTCC AGAAGTTTGA CACATTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT GGCTGGGTCC TCTCCGAAAT CGACAGCCTC 
AACCTTCATA AACAGTGTCA GCTGGGAGTG CTGCCGCTCG GCACAGGGAA CGACTTGGCC 
CGAGTACTGG GCTGGGGCTC AGCCTGCGAT GACGACACCC AGCTCCCCCA GATCTTGGAG 
AAGTTGGAGA GAGCCAGCAC CAAGATGCTG GACAGGTGGA GCGTCATGGC ATACGAGGCC 
AAGCTCCCCC GGCAGGCCTC CTCCTCTACC GTCACCGAAG ACTTCAGCGA GGATTCCGAG 
GTACAGCAGA TTCTCTTCTA TGAAGACTCG GTTGCAGCCC ACCTTTCTAA AATCCTCACC 
TCGGACCAGC ACTCGGTGGT CATCTCCTCG GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC 
TTCGTGGCAC GGGTGGGGAA GGCCTATGAG AAGACGACCG AGAGCTCGGA GGAGTCAGAG 
GTCATGGCCA AGAAGTGCTC TGTCCTGAAA GAGAAGCTGG ATTCCCTTCT CAAGACCTTG 
GACGATGAGT CCCAGGCCTC GTCCTCTCTG CCCAACCCGC CCCCCACCAT TGCCGAGGAG 
GCTGAAGATG GAGATGGGTC GGGCAGCATC TGCGGTTCCA CCGGAGACCG CTTGGTGGCA 
TCAGCTTGCC CGGCCCGGCC GCAGATATTC CGGCCTCGAG AACAGCTCAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGTCAGATC ATAGAACACA CAGAAAAAGC TGTCGATGAG 
CAGAATGCCC AGACCCAGGA GCAGGAGGGC TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG 
AAGATGGACC ACAGAGTGTG CCCACCACTG TCCCACAGCG AGAGCTTCGG GGTCCCCAAG 
GGGAGGAGCC AGCGCAAAGT GTCGAAATCT CCGTGTGAAA AGCTGATCAG CAAAGGGAGT 
CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT 
GCGCTCAACA CCAAGATCCT GTACCCAAAT GTCCGGGCTG GAATGTCTGG TTCCTTACCC 
GGTGGCTCAG TCATCAGTCG CCTGTTAATT AATGCTGATC CCTTCAACTC TGAACCAGAA 
ACCCTAGAGT ATTACACGGA GAAATGTGTC ATGAACAACT ATTTTGGCAT TGGCCTGGAT 
GCGAAGATAT CCCTGGACTT TAACAACAAG CGCGATGAGC ACCCAGAGAA GTGCAGGAGC 
CGAACCAAGA ACATGATGTG GTATGGAGTT CTTGGAACCA AAGAGTTGCT GCACAGAACC 
TACAAGAACC TGGAGCAAAA GGTCTTGCTG GAGTGTGACG GGCGACCCAT CCCACTCCCC 
AGTCTTCAGG GAATTGCTGT CCTTAACATT CCCAGCTATG CCGGAGGAAC CAACTTCTGG 
GGGGGTACCA AGGAAGATGA TACTTTCGCA GCTCCATCAT TCGATGACAA GATTCTGGAG 
GTGGTCGCCG TGTTCGGCAG CATGCAGATG GCCGTCTCTC GAGTCATCAG GCTACAGCAT 
CATCGGATCG CCCAGTGTCG CACGGTGAAG ATCTCCATCC TTGGGGATGA GGGCGTGCCT 
GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG CCGCCAGGGT ACATTCGGAT TGTCCACAAG 
AACCGGGCAC AGACACTGAC CAGAGACAGG GCATTTGAGA GCACCCTGAA GTCCTGGGAA 
GACAAGCAGA AGTGCGAGCT GCCCCGCCCT CCATCCTGTT CCCTGCACCC GGAGATGCTG 
TCCGAGGAGG AGGCCACCCA GATGGACCAG TTTGGGCAGG CAGCAGGGGT CCTCATTCAC 
AGTATCCGAG AAATAGCTCA GTCTCACCGG GACATGGAGC AGGAACTGGC CCACGCCGTC 
AATGCCAGCT CCAAGTCCAT GGACCGTGTG TATGGCAAGC CCAGAACCAC AGAGGGGCTC 
AACTGCAGCT TCGTCCTGGA AATGGTGAAT AACTTCAGAG CTCTGCGCAG TGAGACGGAG 
CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG CTGGATCCGC CTCAGAAGGA GCAGCTGGGG 
AGTGCTCTTG CCGAGATGGA CCGACAGCTC AGGAGGCTGG CAGACACCCC GTGGCTCTGC 
CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT GTGATGCTGG ATCTTGCCAA GCGCAGTCGC 
AGTGGTAAAT TCCGCCTCGT GACCAAGTTT AAAAAGGAGA AAAACAACAA GAACAAAGAA 
GCTCACAGTA GCCTGGGAGC CCCGGTTCAC CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG 
CTGGAGCACC TCAGTCTCTG TGAGTATAAG GACATCTTCA CACGGCACGA CATCCGGGGC 
TCTGAGCTCC TGCACCTGGA GCGGAGGGAC CTCAAGGACC TGGGCGTGAC CAAGGTGGGC 
CACATGAAGA GGATCCTGTG TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_017005140.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860629.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X10, mRNA.

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CloneID	OHu36703
Clone ID Related Accession (Same CDS sequence)	NM_001377259.1 , XM_011512035.1 , XM_017005140.1 , XM_011512040.2 , XM_011512037.2 , XM_005246108.1
Accession Version	XM_017005140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATT GGATTGCAGC ATTAAAGACT GTGCAGAACA GGGAGCACTT TGAGCCCACC 
CAGTACAGCA TGGACCACTT CTCAGGGATG CACAATTGGT ACGCCTGTTC CCACGCGAGG 
CCGACCTACT GCAATGTGTG CCGTGAGGCT CTGTCTGGGG TCACGTCGCA CGGGCTGTCC 
TGCGAGGTGT GCAAATTTAA GGCCCACAAG CGCTGTGCTG TGCGTGCAAC CAATAACTGC 
AAGTGGACCA CACTGGCCTC GATCGGGAAG GACATCATTG AAGATGCAGA TGGGATTGCA 
ATGCCCCACC AGTGGTTGGA AGGAAACCTA CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC 
AAGACCTGTG GCAGTGTGCT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CGTGTAAAGA ATCCTTGCTG ACCAAGTGCC CACTTGGCCT GTGCAAAGTG 
TCAGTCATCC CACCCACGGC TCTCAACAGC ATCGACTCCG ATGGGTTCTG GAAGGCCAGC 
TGTCCTCCTT CTTGCACAAG CCCACTGTTG GTCTTCGTCA ATTCAAAAAG TGGGGACAAC 
CAGGGTGTGA AGTTCCTCAG AAGATTCAAA CAGCTACTAA ACCCCGCCCA GGTCTTCGAC 
CTCATGAACG GAGGCCCACA CCTCGGCTTA CGGTTATTCC AGAAGTTTGA CACATTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT GGCTGGGTCC TCTCCGAAAT CGACAGCCTC 
AACCTTCATA AACAGTGTCA GCTGGGAGTG CTGCCGCTCG GCACAGGGAA CGACTTGGCC 
CGAGTACTGG GCTGGGGCTC AGCCTGCGAT GACGACACCC AGCTCCCCCA GATCTTGGAG 
AAGTTGGAGA GAGCCAGCAC CAAGATGCTG GACAGGTGGA GCGTCATGGC ATACGAGGCC 
AAGCTCCCCC GGCAGGCCTC CTCCTCTACC GTCACCGAAG ACTTCAGCGA GGATTCCGAG 
GTACAGCAGA TTCTCTTCTA TGAAGACTCG GTTGCAGCCC ACCTTTCTAA AATCCTCACC 
TCGGACCAGC ACTCGGTGGT CATCTCCTCG GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC 
TTCGTGGCAC GGGTGGGGAA GGCCTATGAG AAGACGACCG AGAGCTCGGA GGAGTCAGAG 
GTCATGGCCA AGAAGTGCTC TGTCCTGAAA GAGAAGCTGG ATTCCCTTCT CAAGACCTTG 
GACGATGAGT CCCAGGCCTC GTCCTCTCTG CCCAACCCGC CCCCCACCAT TGCCGAGGAG 
GCTGAAGATG GAGATGGGTC GGGCAGCATC TGCGGTTCCA CCGGAGACCG CTTGGTGGCA 
TCAGCTTGCC CGGCCCGGCC GCAGATATTC CGGCCTCGAG AACAGCTCAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGTCAGATC ATAGAACACA CAGAAAAAGC TGTCGATGAG 
CAGAATGCCC AGACCCAGGA GCAGGAGGGC TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG 
AAGATGGACC ACAGAGTGTG CCCACCACTG TCCCACAGCG AGAGCTTCGG GGTCCCCAAG 
GGGAGGAGCC AGCGCAAAGT GTCGAAATCT CCGTGTGAAA AGCTGATCAG CAAAGGGAGT 
CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT 
GCGCTCAACA CCAAGATCCT GTACCCAAAT GTCCGGGCTG GAATGTCTGG TTCCTTACCC 
GGTGGCTCAG TCATCAGTCG CCTGTTAATT AATGCTGATC CCTTCAACTC TGAACCAGAA 
ACCCTAGAGT ATTACACGGA GAAATGTGTC ATGAACAACT ATTTTGGCAT TGGCCTGGAT 
GCGAAGATAT CCCTGGACTT TAACAACAAG CGCGATGAGC ACCCAGAGAA GTGCAGGAGC 
CGAACCAAGA ACATGATGTG GTATGGAGTT CTTGGAACCA AAGAGTTGCT GCACAGAACC 
TACAAGAACC TGGAGCAAAA GGTCTTGCTG GAGTGTGACG GGCGACCCAT CCCACTCCCC 
AGTCTTCAGG GAATTGCTGT CCTTAACATT CCCAGCTATG CCGGAGGAAC CAACTTCTGG 
GGGGGTACCA AGGAAGATGA TACTTTCGCA GCTCCATCAT TCGATGACAA GATTCTGGAG 
GTGGTCGCCG TGTTCGGCAG CATGCAGATG GCCGTCTCTC GAGTCATCAG GCTACAGCAT 
CATCGGATCG CCCAGTGTCG CACGGTGAAG ATCTCCATCC TTGGGGATGA GGGCGTGCCT 
GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG CCGCCAGGGT ACATTCGGAT TGTCCACAAG 
AACCGGGCAC AGACACTGAC CAGAGACAGG GCATTTGAGA GCACCCTGAA GTCCTGGGAA 
GACAAGCAGA AGTGCGAGCT GCCCCGCCCT CCATCCTGTT CCCTGCACCC GGAGATGCTG 
TCCGAGGAGG AGGCCACCCA GATGGACCAG TTTGGGCAGG CAGCAGGGGT CCTCATTCAC 
AGTATCCGAG AAATAGCTCA GTCTCACCGG GACATGGAGC AGGAACTGGC CCACGCCGTC 
AATGCCAGCT CCAAGTCCAT GGACCGTGTG TATGGCAAGC CCAGAACCAC AGAGGGGCTC 
AACTGCAGCT TCGTCCTGGA AATGGTGAAT AACTTCAGAG CTCTGCGCAG TGAGACGGAG 
CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG CTGGATCCGC CTCAGAAGGA GCAGCTGGGG 
AGTGCTCTTG CCGAGATGGA CCGACAGCTC AGGAGGCTGG CAGACACCCC GTGGCTCTGC 
CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT GTGATGCTGG ATCTTGCCAA GCGCAGTCGC 
AGTGGTAAAT TCCGCCTCGT GACCAAGTTT AAAAAGGAGA AAAACAACAA GAACAAAGAA 
GCTCACAGTA GCCTGGGAGC CCCGGTTCAC CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG 
CTGGAGCACC TCAGTCTCTG TGAGTATAAG GACATCTTCA CACGGCACGA CATCCGGGGC 
TCTGAGCTCC TGCACCTGGA GCGGAGGGAC CTCAAGGACC TGGGCGTGAC CAAGGTGGGC 
CACATGAAGA GGATCCTGTG TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860629.1
CDS	344..3589
Translation	MEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_017005140.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005140.1

ORF Insert Sequence:

ATGGAAGATT GGATTGCAGC ATTAAAGACT GTGCAGAACA GGGAGCACTT TGAGCCCACC 
CAGTACAGCA TGGACCACTT CTCAGGGATG CACAATTGGT ACGCCTGTTC CCACGCGAGG 
CCGACCTACT GCAATGTGTG CCGTGAGGCT CTGTCTGGGG TCACGTCGCA CGGGCTGTCC 
TGCGAGGTGT GCAAATTTAA GGCCCACAAG CGCTGTGCTG TGCGTGCAAC CAATAACTGC 
AAGTGGACCA CACTGGCCTC GATCGGGAAG GACATCATTG AAGATGCAGA TGGGATTGCA 
ATGCCCCACC AGTGGTTGGA AGGAAACCTA CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC 
AAGACCTGTG GCAGTGTGCT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CGTGTAAAGA ATCCTTGCTG ACCAAGTGCC CACTTGGCCT GTGCAAAGTG 
TCAGTCATCC CACCCACGGC TCTCAACAGC ATCGACTCCG ATGGGTTCTG GAAGGCCAGC 
TGTCCTCCTT CTTGCACAAG CCCACTGTTG GTCTTCGTCA ATTCAAAAAG TGGGGACAAC 
CAGGGTGTGA AGTTCCTCAG AAGATTCAAA CAGCTACTAA ACCCCGCCCA GGTCTTCGAC 
CTCATGAACG GAGGCCCACA CCTCGGCTTA CGGTTATTCC AGAAGTTTGA CACATTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT GGCTGGGTCC TCTCCGAAAT CGACAGCCTC 
AACCTTCATA AACAGTGTCA GCTGGGAGTG CTGCCGCTCG GCACAGGGAA CGACTTGGCC 
CGAGTACTGG GCTGGGGCTC AGCCTGCGAT GACGACACCC AGCTCCCCCA GATCTTGGAG 
AAGTTGGAGA GAGCCAGCAC CAAGATGCTG GACAGGTGGA GCGTCATGGC ATACGAGGCC 
AAGCTCCCCC GGCAGGCCTC CTCCTCTACC GTCACCGAAG ACTTCAGCGA GGATTCCGAG 
GTACAGCAGA TTCTCTTCTA TGAAGACTCG GTTGCAGCCC ACCTTTCTAA AATCCTCACC 
TCGGACCAGC ACTCGGTGGT CATCTCCTCG GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC 
TTCGTGGCAC GGGTGGGGAA GGCCTATGAG AAGACGACCG AGAGCTCGGA GGAGTCAGAG 
GTCATGGCCA AGAAGTGCTC TGTCCTGAAA GAGAAGCTGG ATTCCCTTCT CAAGACCTTG 
GACGATGAGT CCCAGGCCTC GTCCTCTCTG CCCAACCCGC CCCCCACCAT TGCCGAGGAG 
GCTGAAGATG GAGATGGGTC GGGCAGCATC TGCGGTTCCA CCGGAGACCG CTTGGTGGCA 
TCAGCTTGCC CGGCCCGGCC GCAGATATTC CGGCCTCGAG AACAGCTCAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGTCAGATC ATAGAACACA CAGAAAAAGC TGTCGATGAG 
CAGAATGCCC AGACCCAGGA GCAGGAGGGC TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG 
AAGATGGACC ACAGAGTGTG CCCACCACTG TCCCACAGCG AGAGCTTCGG GGTCCCCAAG 
GGGAGGAGCC AGCGCAAAGT GTCGAAATCT CCGTGTGAAA AGCTGATCAG CAAAGGGAGT 
CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT 
GCGCTCAACA CCAAGATCCT GTACCCAAAT GTCCGGGCTG GAATGTCTGG TTCCTTACCC 
GGTGGCTCAG TCATCAGTCG CCTGTTAATT AATGCTGATC CCTTCAACTC TGAACCAGAA 
ACCCTAGAGT ATTACACGGA GAAATGTGTC ATGAACAACT ATTTTGGCAT TGGCCTGGAT 
GCGAAGATAT CCCTGGACTT TAACAACAAG CGCGATGAGC ACCCAGAGAA GTGCAGGAGC 
CGAACCAAGA ACATGATGTG GTATGGAGTT CTTGGAACCA AAGAGTTGCT GCACAGAACC 
TACAAGAACC TGGAGCAAAA GGTCTTGCTG GAGTGTGACG GGCGACCCAT CCCACTCCCC 
AGTCTTCAGG GAATTGCTGT CCTTAACATT CCCAGCTATG CCGGAGGAAC CAACTTCTGG 
GGGGGTACCA AGGAAGATGA TACTTTCGCA GCTCCATCAT TCGATGACAA GATTCTGGAG 
GTGGTCGCCG TGTTCGGCAG CATGCAGATG GCCGTCTCTC GAGTCATCAG GCTACAGCAT 
CATCGGATCG CCCAGTGTCG CACGGTGAAG ATCTCCATCC TTGGGGATGA GGGCGTGCCT 
GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG CCGCCAGGGT ACATTCGGAT TGTCCACAAG 
AACCGGGCAC AGACACTGAC CAGAGACAGG GCATTTGAGA GCACCCTGAA GTCCTGGGAA 
GACAAGCAGA AGTGCGAGCT GCCCCGCCCT CCATCCTGTT CCCTGCACCC GGAGATGCTG 
TCCGAGGAGG AGGCCACCCA GATGGACCAG TTTGGGCAGG CAGCAGGGGT CCTCATTCAC 
AGTATCCGAG AAATAGCTCA GTCTCACCGG GACATGGAGC AGGAACTGGC CCACGCCGTC 
AATGCCAGCT CCAAGTCCAT GGACCGTGTG TATGGCAAGC CCAGAACCAC AGAGGGGCTC 
AACTGCAGCT TCGTCCTGGA AATGGTGAAT AACTTCAGAG CTCTGCGCAG TGAGACGGAG 
CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG CTGGATCCGC CTCAGAAGGA GCAGCTGGGG 
AGTGCTCTTG CCGAGATGGA CCGACAGCTC AGGAGGCTGG CAGACACCCC GTGGCTCTGC 
CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT GTGATGCTGG ATCTTGCCAA GCGCAGTCGC 
AGTGGTAAAT TCCGCCTCGT GACCAAGTTT AAAAAGGAGA AAAACAACAA GAACAAAGAA 
GCTCACAGTA GCCTGGGAGC CCCGGTTCAC CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG 
CTGGAGCACC TCAGTCTCTG TGAGTATAAG GACATCTTCA CACGGCACGA CATCCGGGGC 
TCTGAGCTCC TGCACCTGGA GCGGAGGGAC CTCAAGGACC TGGGCGTGAC CAAGGTGGGC 
CACATGAAGA GGATCCTGTG TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512040.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510342.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X15, mRNA.

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CloneID	OHu36703
Clone ID Related Accession (Same CDS sequence)	NM_001377259.1 , XM_011512035.1 , XM_017005140.1 , XM_011512040.2 , XM_011512037.2 , XM_005246108.1
Accession Version	XM_011512040.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512040.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATT GGATTGCAGC ATTAAAGACT GTGCAGAACA GGGAGCACTT TGAGCCCACC 
CAGTACAGCA TGGACCACTT CTCAGGGATG CACAATTGGT ACGCCTGTTC CCACGCGAGG 
CCGACCTACT GCAATGTGTG CCGTGAGGCT CTGTCTGGGG TCACGTCGCA CGGGCTGTCC 
TGCGAGGTGT GCAAATTTAA GGCCCACAAG CGCTGTGCTG TGCGTGCAAC CAATAACTGC 
AAGTGGACCA CACTGGCCTC GATCGGGAAG GACATCATTG AAGATGCAGA TGGGATTGCA 
ATGCCCCACC AGTGGTTGGA AGGAAACCTA CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC 
AAGACCTGTG GCAGTGTGCT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CGTGTAAAGA ATCCTTGCTG ACCAAGTGCC CACTTGGCCT GTGCAAAGTG 
TCAGTCATCC CACCCACGGC TCTCAACAGC ATCGACTCCG ATGGGTTCTG GAAGGCCAGC 
TGTCCTCCTT CTTGCACAAG CCCACTGTTG GTCTTCGTCA ATTCAAAAAG TGGGGACAAC 
CAGGGTGTGA AGTTCCTCAG AAGATTCAAA CAGCTACTAA ACCCCGCCCA GGTCTTCGAC 
CTCATGAACG GAGGCCCACA CCTCGGCTTA CGGTTATTCC AGAAGTTTGA CACATTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT GGCTGGGTCC TCTCCGAAAT CGACAGCCTC 
AACCTTCATA AACAGTGTCA GCTGGGAGTG CTGCCGCTCG GCACAGGGAA CGACTTGGCC 
CGAGTACTGG GCTGGGGCTC AGCCTGCGAT GACGACACCC AGCTCCCCCA GATCTTGGAG 
AAGTTGGAGA GAGCCAGCAC CAAGATGCTG GACAGGTGGA GCGTCATGGC ATACGAGGCC 
AAGCTCCCCC GGCAGGCCTC CTCCTCTACC GTCACCGAAG ACTTCAGCGA GGATTCCGAG 
GTACAGCAGA TTCTCTTCTA TGAAGACTCG GTTGCAGCCC ACCTTTCTAA AATCCTCACC 
TCGGACCAGC ACTCGGTGGT CATCTCCTCG GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC 
TTCGTGGCAC GGGTGGGGAA GGCCTATGAG AAGACGACCG AGAGCTCGGA GGAGTCAGAG 
GTCATGGCCA AGAAGTGCTC TGTCCTGAAA GAGAAGCTGG ATTCCCTTCT CAAGACCTTG 
GACGATGAGT CCCAGGCCTC GTCCTCTCTG CCCAACCCGC CCCCCACCAT TGCCGAGGAG 
GCTGAAGATG GAGATGGGTC GGGCAGCATC TGCGGTTCCA CCGGAGACCG CTTGGTGGCA 
TCAGCTTGCC CGGCCCGGCC GCAGATATTC CGGCCTCGAG AACAGCTCAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGTCAGATC ATAGAACACA CAGAAAAAGC TGTCGATGAG 
CAGAATGCCC AGACCCAGGA GCAGGAGGGC TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG 
AAGATGGACC ACAGAGTGTG CCCACCACTG TCCCACAGCG AGAGCTTCGG GGTCCCCAAG 
GGGAGGAGCC AGCGCAAAGT GTCGAAATCT CCGTGTGAAA AGCTGATCAG CAAAGGGAGT 
CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT 
GCGCTCAACA CCAAGATCCT GTACCCAAAT GTCCGGGCTG GAATGTCTGG TTCCTTACCC 
GGTGGCTCAG TCATCAGTCG CCTGTTAATT AATGCTGATC CCTTCAACTC TGAACCAGAA 
ACCCTAGAGT ATTACACGGA GAAATGTGTC ATGAACAACT ATTTTGGCAT TGGCCTGGAT 
GCGAAGATAT CCCTGGACTT TAACAACAAG CGCGATGAGC ACCCAGAGAA GTGCAGGAGC 
CGAACCAAGA ACATGATGTG GTATGGAGTT CTTGGAACCA AAGAGTTGCT GCACAGAACC 
TACAAGAACC TGGAGCAAAA GGTCTTGCTG GAGTGTGACG GGCGACCCAT CCCACTCCCC 
AGTCTTCAGG GAATTGCTGT CCTTAACATT CCCAGCTATG CCGGAGGAAC CAACTTCTGG 
GGGGGTACCA AGGAAGATGA TACTTTCGCA GCTCCATCAT TCGATGACAA GATTCTGGAG 
GTGGTCGCCG TGTTCGGCAG CATGCAGATG GCCGTCTCTC GAGTCATCAG GCTACAGCAT 
CATCGGATCG CCCAGTGTCG CACGGTGAAG ATCTCCATCC TTGGGGATGA GGGCGTGCCT 
GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG CCGCCAGGGT ACATTCGGAT TGTCCACAAG 
AACCGGGCAC AGACACTGAC CAGAGACAGG GCATTTGAGA GCACCCTGAA GTCCTGGGAA 
GACAAGCAGA AGTGCGAGCT GCCCCGCCCT CCATCCTGTT CCCTGCACCC GGAGATGCTG 
TCCGAGGAGG AGGCCACCCA GATGGACCAG TTTGGGCAGG CAGCAGGGGT CCTCATTCAC 
AGTATCCGAG AAATAGCTCA GTCTCACCGG GACATGGAGC AGGAACTGGC CCACGCCGTC 
AATGCCAGCT CCAAGTCCAT GGACCGTGTG TATGGCAAGC CCAGAACCAC AGAGGGGCTC 
AACTGCAGCT TCGTCCTGGA AATGGTGAAT AACTTCAGAG CTCTGCGCAG TGAGACGGAG 
CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG CTGGATCCGC CTCAGAAGGA GCAGCTGGGG 
AGTGCTCTTG CCGAGATGGA CCGACAGCTC AGGAGGCTGG CAGACACCCC GTGGCTCTGC 
CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT GTGATGCTGG ATCTTGCCAA GCGCAGTCGC 
AGTGGTAAAT TCCGCCTCGT GACCAAGTTT AAAAAGGAGA AAAACAACAA GAACAAAGAA 
GCTCACAGTA GCCTGGGAGC CCCGGTTCAC CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG 
CTGGAGCACC TCAGTCTCTG TGAGTATAAG GACATCTTCA CACGGCACGA CATCCGGGGC 
TCTGAGCTCC TGCACCTGGA GCGGAGGGAC CTCAAGGACC TGGGCGTGAC CAAGGTGGGC 
CACATGAAGA GGATCCTGTG TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510342.1
CDS	249..3494
Translation	MEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_011512040.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512040.2

ORF Insert Sequence:

ATGGAAGATT GGATTGCAGC ATTAAAGACT GTGCAGAACA GGGAGCACTT TGAGCCCACC 
CAGTACAGCA TGGACCACTT CTCAGGGATG CACAATTGGT ACGCCTGTTC CCACGCGAGG 
CCGACCTACT GCAATGTGTG CCGTGAGGCT CTGTCTGGGG TCACGTCGCA CGGGCTGTCC 
TGCGAGGTGT GCAAATTTAA GGCCCACAAG CGCTGTGCTG TGCGTGCAAC CAATAACTGC 
AAGTGGACCA CACTGGCCTC GATCGGGAAG GACATCATTG AAGATGCAGA TGGGATTGCA 
ATGCCCCACC AGTGGTTGGA AGGAAACCTA CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC 
AAGACCTGTG GCAGTGTGCT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CGTGTAAAGA ATCCTTGCTG ACCAAGTGCC CACTTGGCCT GTGCAAAGTG 
TCAGTCATCC CACCCACGGC TCTCAACAGC ATCGACTCCG ATGGGTTCTG GAAGGCCAGC 
TGTCCTCCTT CTTGCACAAG CCCACTGTTG GTCTTCGTCA ATTCAAAAAG TGGGGACAAC 
CAGGGTGTGA AGTTCCTCAG AAGATTCAAA CAGCTACTAA ACCCCGCCCA GGTCTTCGAC 
CTCATGAACG GAGGCCCACA CCTCGGCTTA CGGTTATTCC AGAAGTTTGA CACATTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT GGCTGGGTCC TCTCCGAAAT CGACAGCCTC 
AACCTTCATA AACAGTGTCA GCTGGGAGTG CTGCCGCTCG GCACAGGGAA CGACTTGGCC 
CGAGTACTGG GCTGGGGCTC AGCCTGCGAT GACGACACCC AGCTCCCCCA GATCTTGGAG 
AAGTTGGAGA GAGCCAGCAC CAAGATGCTG GACAGGTGGA GCGTCATGGC ATACGAGGCC 
AAGCTCCCCC GGCAGGCCTC CTCCTCTACC GTCACCGAAG ACTTCAGCGA GGATTCCGAG 
GTACAGCAGA TTCTCTTCTA TGAAGACTCG GTTGCAGCCC ACCTTTCTAA AATCCTCACC 
TCGGACCAGC ACTCGGTGGT CATCTCCTCG GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC 
TTCGTGGCAC GGGTGGGGAA GGCCTATGAG AAGACGACCG AGAGCTCGGA GGAGTCAGAG 
GTCATGGCCA AGAAGTGCTC TGTCCTGAAA GAGAAGCTGG ATTCCCTTCT CAAGACCTTG 
GACGATGAGT CCCAGGCCTC GTCCTCTCTG CCCAACCCGC CCCCCACCAT TGCCGAGGAG 
GCTGAAGATG GAGATGGGTC GGGCAGCATC TGCGGTTCCA CCGGAGACCG CTTGGTGGCA 
TCAGCTTGCC CGGCCCGGCC GCAGATATTC CGGCCTCGAG AACAGCTCAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGTCAGATC ATAGAACACA CAGAAAAAGC TGTCGATGAG 
CAGAATGCCC AGACCCAGGA GCAGGAGGGC TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG 
AAGATGGACC ACAGAGTGTG CCCACCACTG TCCCACAGCG AGAGCTTCGG GGTCCCCAAG 
GGGAGGAGCC AGCGCAAAGT GTCGAAATCT CCGTGTGAAA AGCTGATCAG CAAAGGGAGT 
CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT 
GCGCTCAACA CCAAGATCCT GTACCCAAAT GTCCGGGCTG GAATGTCTGG TTCCTTACCC 
GGTGGCTCAG TCATCAGTCG CCTGTTAATT AATGCTGATC CCTTCAACTC TGAACCAGAA 
ACCCTAGAGT ATTACACGGA GAAATGTGTC ATGAACAACT ATTTTGGCAT TGGCCTGGAT 
GCGAAGATAT CCCTGGACTT TAACAACAAG CGCGATGAGC ACCCAGAGAA GTGCAGGAGC 
CGAACCAAGA ACATGATGTG GTATGGAGTT CTTGGAACCA AAGAGTTGCT GCACAGAACC 
TACAAGAACC TGGAGCAAAA GGTCTTGCTG GAGTGTGACG GGCGACCCAT CCCACTCCCC 
AGTCTTCAGG GAATTGCTGT CCTTAACATT CCCAGCTATG CCGGAGGAAC CAACTTCTGG 
GGGGGTACCA AGGAAGATGA TACTTTCGCA GCTCCATCAT TCGATGACAA GATTCTGGAG 
GTGGTCGCCG TGTTCGGCAG CATGCAGATG GCCGTCTCTC GAGTCATCAG GCTACAGCAT 
CATCGGATCG CCCAGTGTCG CACGGTGAAG ATCTCCATCC TTGGGGATGA GGGCGTGCCT 
GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG CCGCCAGGGT ACATTCGGAT TGTCCACAAG 
AACCGGGCAC AGACACTGAC CAGAGACAGG GCATTTGAGA GCACCCTGAA GTCCTGGGAA 
GACAAGCAGA AGTGCGAGCT GCCCCGCCCT CCATCCTGTT CCCTGCACCC GGAGATGCTG 
TCCGAGGAGG AGGCCACCCA GATGGACCAG TTTGGGCAGG CAGCAGGGGT CCTCATTCAC 
AGTATCCGAG AAATAGCTCA GTCTCACCGG GACATGGAGC AGGAACTGGC CCACGCCGTC 
AATGCCAGCT CCAAGTCCAT GGACCGTGTG TATGGCAAGC CCAGAACCAC AGAGGGGCTC 
AACTGCAGCT TCGTCCTGGA AATGGTGAAT AACTTCAGAG CTCTGCGCAG TGAGACGGAG 
CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG CTGGATCCGC CTCAGAAGGA GCAGCTGGGG 
AGTGCTCTTG CCGAGATGGA CCGACAGCTC AGGAGGCTGG CAGACACCCC GTGGCTCTGC 
CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT GTGATGCTGG ATCTTGCCAA GCGCAGTCGC 
AGTGGTAAAT TCCGCCTCGT GACCAAGTTT AAAAAGGAGA AAAACAACAA GAACAAAGAA 
GCTCACAGTA GCCTGGGAGC CCCGGTTCAC CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG 
CTGGAGCACC TCAGTCTCTG TGAGTATAAG GACATCTTCA CACGGCACGA CATCCGGGGC 
TCTGAGCTCC TGCACCTGGA GCGGAGGGAC CTCAAGGACC TGGGCGTGAC CAAGGTGGGC 
CACATGAAGA GGATCCTGTG TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512037.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510339.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu36703
Clone ID Related Accession (Same CDS sequence)	NM_001377259.1 , XM_011512035.1 , XM_017005140.1 , XM_011512040.2 , XM_011512037.2 , XM_005246108.1
Accession Version	XM_011512037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATT GGATTGCAGC ATTAAAGACT GTGCAGAACA GGGAGCACTT TGAGCCCACC 
CAGTACAGCA TGGACCACTT CTCAGGGATG CACAATTGGT ACGCCTGTTC CCACGCGAGG 
CCGACCTACT GCAATGTGTG CCGTGAGGCT CTGTCTGGGG TCACGTCGCA CGGGCTGTCC 
TGCGAGGTGT GCAAATTTAA GGCCCACAAG CGCTGTGCTG TGCGTGCAAC CAATAACTGC 
AAGTGGACCA CACTGGCCTC GATCGGGAAG GACATCATTG AAGATGCAGA TGGGATTGCA 
ATGCCCCACC AGTGGTTGGA AGGAAACCTA CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC 
AAGACCTGTG GCAGTGTGCT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CGTGTAAAGA ATCCTTGCTG ACCAAGTGCC CACTTGGCCT GTGCAAAGTG 
TCAGTCATCC CACCCACGGC TCTCAACAGC ATCGACTCCG ATGGGTTCTG GAAGGCCAGC 
TGTCCTCCTT CTTGCACAAG CCCACTGTTG GTCTTCGTCA ATTCAAAAAG TGGGGACAAC 
CAGGGTGTGA AGTTCCTCAG AAGATTCAAA CAGCTACTAA ACCCCGCCCA GGTCTTCGAC 
CTCATGAACG GAGGCCCACA CCTCGGCTTA CGGTTATTCC AGAAGTTTGA CACATTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT GGCTGGGTCC TCTCCGAAAT CGACAGCCTC 
AACCTTCATA AACAGTGTCA GCTGGGAGTG CTGCCGCTCG GCACAGGGAA CGACTTGGCC 
CGAGTACTGG GCTGGGGCTC AGCCTGCGAT GACGACACCC AGCTCCCCCA GATCTTGGAG 
AAGTTGGAGA GAGCCAGCAC CAAGATGCTG GACAGGTGGA GCGTCATGGC ATACGAGGCC 
AAGCTCCCCC GGCAGGCCTC CTCCTCTACC GTCACCGAAG ACTTCAGCGA GGATTCCGAG 
GTACAGCAGA TTCTCTTCTA TGAAGACTCG GTTGCAGCCC ACCTTTCTAA AATCCTCACC 
TCGGACCAGC ACTCGGTGGT CATCTCCTCG GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC 
TTCGTGGCAC GGGTGGGGAA GGCCTATGAG AAGACGACCG AGAGCTCGGA GGAGTCAGAG 
GTCATGGCCA AGAAGTGCTC TGTCCTGAAA GAGAAGCTGG ATTCCCTTCT CAAGACCTTG 
GACGATGAGT CCCAGGCCTC GTCCTCTCTG CCCAACCCGC CCCCCACCAT TGCCGAGGAG 
GCTGAAGATG GAGATGGGTC GGGCAGCATC TGCGGTTCCA CCGGAGACCG CTTGGTGGCA 
TCAGCTTGCC CGGCCCGGCC GCAGATATTC CGGCCTCGAG AACAGCTCAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGTCAGATC ATAGAACACA CAGAAAAAGC TGTCGATGAG 
CAGAATGCCC AGACCCAGGA GCAGGAGGGC TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG 
AAGATGGACC ACAGAGTGTG CCCACCACTG TCCCACAGCG AGAGCTTCGG GGTCCCCAAG 
GGGAGGAGCC AGCGCAAAGT GTCGAAATCT CCGTGTGAAA AGCTGATCAG CAAAGGGAGT 
CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT 
GCGCTCAACA CCAAGATCCT GTACCCAAAT GTCCGGGCTG GAATGTCTGG TTCCTTACCC 
GGTGGCTCAG TCATCAGTCG CCTGTTAATT AATGCTGATC CCTTCAACTC TGAACCAGAA 
ACCCTAGAGT ATTACACGGA GAAATGTGTC ATGAACAACT ATTTTGGCAT TGGCCTGGAT 
GCGAAGATAT CCCTGGACTT TAACAACAAG CGCGATGAGC ACCCAGAGAA GTGCAGGAGC 
CGAACCAAGA ACATGATGTG GTATGGAGTT CTTGGAACCA AAGAGTTGCT GCACAGAACC 
TACAAGAACC TGGAGCAAAA GGTCTTGCTG GAGTGTGACG GGCGACCCAT CCCACTCCCC 
AGTCTTCAGG GAATTGCTGT CCTTAACATT CCCAGCTATG CCGGAGGAAC CAACTTCTGG 
GGGGGTACCA AGGAAGATGA TACTTTCGCA GCTCCATCAT TCGATGACAA GATTCTGGAG 
GTGGTCGCCG TGTTCGGCAG CATGCAGATG GCCGTCTCTC GAGTCATCAG GCTACAGCAT 
CATCGGATCG CCCAGTGTCG CACGGTGAAG ATCTCCATCC TTGGGGATGA GGGCGTGCCT 
GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG CCGCCAGGGT ACATTCGGAT TGTCCACAAG 
AACCGGGCAC AGACACTGAC CAGAGACAGG GCATTTGAGA GCACCCTGAA GTCCTGGGAA 
GACAAGCAGA AGTGCGAGCT GCCCCGCCCT CCATCCTGTT CCCTGCACCC GGAGATGCTG 
TCCGAGGAGG AGGCCACCCA GATGGACCAG TTTGGGCAGG CAGCAGGGGT CCTCATTCAC 
AGTATCCGAG AAATAGCTCA GTCTCACCGG GACATGGAGC AGGAACTGGC CCACGCCGTC 
AATGCCAGCT CCAAGTCCAT GGACCGTGTG TATGGCAAGC CCAGAACCAC AGAGGGGCTC 
AACTGCAGCT TCGTCCTGGA AATGGTGAAT AACTTCAGAG CTCTGCGCAG TGAGACGGAG 
CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG CTGGATCCGC CTCAGAAGGA GCAGCTGGGG 
AGTGCTCTTG CCGAGATGGA CCGACAGCTC AGGAGGCTGG CAGACACCCC GTGGCTCTGC 
CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT GTGATGCTGG ATCTTGCCAA GCGCAGTCGC 
AGTGGTAAAT TCCGCCTCGT GACCAAGTTT AAAAAGGAGA AAAACAACAA GAACAAAGAA 
GCTCACAGTA GCCTGGGAGC CCCGGTTCAC CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG 
CTGGAGCACC TCAGTCTCTG TGAGTATAAG GACATCTTCA CACGGCACGA CATCCGGGGC 
TCTGAGCTCC TGCACCTGGA GCGGAGGGAC CTCAAGGACC TGGGCGTGAC CAAGGTGGGC 
CACATGAAGA GGATCCTGTG TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510339.1
CDS	317..3562
Translation	MEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_011512037.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512037.2

ORF Insert Sequence:

ATGGAAGATT GGATTGCAGC ATTAAAGACT GTGCAGAACA GGGAGCACTT TGAGCCCACC 
CAGTACAGCA TGGACCACTT CTCAGGGATG CACAATTGGT ACGCCTGTTC CCACGCGAGG 
CCGACCTACT GCAATGTGTG CCGTGAGGCT CTGTCTGGGG TCACGTCGCA CGGGCTGTCC 
TGCGAGGTGT GCAAATTTAA GGCCCACAAG CGCTGTGCTG TGCGTGCAAC CAATAACTGC 
AAGTGGACCA CACTGGCCTC GATCGGGAAG GACATCATTG AAGATGCAGA TGGGATTGCA 
ATGCCCCACC AGTGGTTGGA AGGAAACCTA CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC 
AAGACCTGTG GCAGTGTGCT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CGTGTAAAGA ATCCTTGCTG ACCAAGTGCC CACTTGGCCT GTGCAAAGTG 
TCAGTCATCC CACCCACGGC TCTCAACAGC ATCGACTCCG ATGGGTTCTG GAAGGCCAGC 
TGTCCTCCTT CTTGCACAAG CCCACTGTTG GTCTTCGTCA ATTCAAAAAG TGGGGACAAC 
CAGGGTGTGA AGTTCCTCAG AAGATTCAAA CAGCTACTAA ACCCCGCCCA GGTCTTCGAC 
CTCATGAACG GAGGCCCACA CCTCGGCTTA CGGTTATTCC AGAAGTTTGA CACATTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT GGCTGGGTCC TCTCCGAAAT CGACAGCCTC 
AACCTTCATA AACAGTGTCA GCTGGGAGTG CTGCCGCTCG GCACAGGGAA CGACTTGGCC 
CGAGTACTGG GCTGGGGCTC AGCCTGCGAT GACGACACCC AGCTCCCCCA GATCTTGGAG 
AAGTTGGAGA GAGCCAGCAC CAAGATGCTG GACAGGTGGA GCGTCATGGC ATACGAGGCC 
AAGCTCCCCC GGCAGGCCTC CTCCTCTACC GTCACCGAAG ACTTCAGCGA GGATTCCGAG 
GTACAGCAGA TTCTCTTCTA TGAAGACTCG GTTGCAGCCC ACCTTTCTAA AATCCTCACC 
TCGGACCAGC ACTCGGTGGT CATCTCCTCG GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC 
TTCGTGGCAC GGGTGGGGAA GGCCTATGAG AAGACGACCG AGAGCTCGGA GGAGTCAGAG 
GTCATGGCCA AGAAGTGCTC TGTCCTGAAA GAGAAGCTGG ATTCCCTTCT CAAGACCTTG 
GACGATGAGT CCCAGGCCTC GTCCTCTCTG CCCAACCCGC CCCCCACCAT TGCCGAGGAG 
GCTGAAGATG GAGATGGGTC GGGCAGCATC TGCGGTTCCA CCGGAGACCG CTTGGTGGCA 
TCAGCTTGCC CGGCCCGGCC GCAGATATTC CGGCCTCGAG AACAGCTCAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGTCAGATC ATAGAACACA CAGAAAAAGC TGTCGATGAG 
CAGAATGCCC AGACCCAGGA GCAGGAGGGC TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG 
AAGATGGACC ACAGAGTGTG CCCACCACTG TCCCACAGCG AGAGCTTCGG GGTCCCCAAG 
GGGAGGAGCC AGCGCAAAGT GTCGAAATCT CCGTGTGAAA AGCTGATCAG CAAAGGGAGT 
CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT 
GCGCTCAACA CCAAGATCCT GTACCCAAAT GTCCGGGCTG GAATGTCTGG TTCCTTACCC 
GGTGGCTCAG TCATCAGTCG CCTGTTAATT AATGCTGATC CCTTCAACTC TGAACCAGAA 
ACCCTAGAGT ATTACACGGA GAAATGTGTC ATGAACAACT ATTTTGGCAT TGGCCTGGAT 
GCGAAGATAT CCCTGGACTT TAACAACAAG CGCGATGAGC ACCCAGAGAA GTGCAGGAGC 
CGAACCAAGA ACATGATGTG GTATGGAGTT CTTGGAACCA AAGAGTTGCT GCACAGAACC 
TACAAGAACC TGGAGCAAAA GGTCTTGCTG GAGTGTGACG GGCGACCCAT CCCACTCCCC 
AGTCTTCAGG GAATTGCTGT CCTTAACATT CCCAGCTATG CCGGAGGAAC CAACTTCTGG 
GGGGGTACCA AGGAAGATGA TACTTTCGCA GCTCCATCAT TCGATGACAA GATTCTGGAG 
GTGGTCGCCG TGTTCGGCAG CATGCAGATG GCCGTCTCTC GAGTCATCAG GCTACAGCAT 
CATCGGATCG CCCAGTGTCG CACGGTGAAG ATCTCCATCC TTGGGGATGA GGGCGTGCCT 
GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG CCGCCAGGGT ACATTCGGAT TGTCCACAAG 
AACCGGGCAC AGACACTGAC CAGAGACAGG GCATTTGAGA GCACCCTGAA GTCCTGGGAA 
GACAAGCAGA AGTGCGAGCT GCCCCGCCCT CCATCCTGTT CCCTGCACCC GGAGATGCTG 
TCCGAGGAGG AGGCCACCCA GATGGACCAG TTTGGGCAGG CAGCAGGGGT CCTCATTCAC 
AGTATCCGAG AAATAGCTCA GTCTCACCGG GACATGGAGC AGGAACTGGC CCACGCCGTC 
AATGCCAGCT CCAAGTCCAT GGACCGTGTG TATGGCAAGC CCAGAACCAC AGAGGGGCTC 
AACTGCAGCT TCGTCCTGGA AATGGTGAAT AACTTCAGAG CTCTGCGCAG TGAGACGGAG 
CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG CTGGATCCGC CTCAGAAGGA GCAGCTGGGG 
AGTGCTCTTG CCGAGATGGA CCGACAGCTC AGGAGGCTGG CAGACACCCC GTGGCTCTGC 
CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT GTGATGCTGG ATCTTGCCAA GCGCAGTCGC 
AGTGGTAAAT TCCGCCTCGT GACCAAGTTT AAAAAGGAGA AAAACAACAA GAACAAAGAA 
GCTCACAGTA GCCTGGGAGC CCCGGTTCAC CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG 
CTGGAGCACC TCAGTCTCTG TGAGTATAAG GACATCTTCA CACGGCACGA CATCCGGGGC 
TCTGAGCTCC TGCACCTGGA GCGGAGGGAC CTCAAGGACC TGGGCGTGAC CAAGGTGGGC 
CACATGAAGA GGATCCTGTG TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_005246108.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246165.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X11, mRNA.

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CloneID	OHu36703
Clone ID Related Accession (Same CDS sequence)	NM_001377259.1 , XM_011512035.1 , XM_017005140.1 , XM_011512040.2 , XM_011512037.2 , XM_005246108.1
Accession Version	XM_005246108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGAAGATT GGATTGCAGC ATTAAAGACT GTGCAGAACA GGGAGCACTT TGAGCCCACC 
CAGTACAGCA TGGACCACTT CTCAGGGATG CACAATTGGT ACGCCTGTTC CCACGCGAGG 
CCGACCTACT GCAATGTGTG CCGTGAGGCT CTGTCTGGGG TCACGTCGCA CGGGCTGTCC 
TGCGAGGTGT GCAAATTTAA GGCCCACAAG CGCTGTGCTG TGCGTGCAAC CAATAACTGC 
AAGTGGACCA CACTGGCCTC GATCGGGAAG GACATCATTG AAGATGCAGA TGGGATTGCA 
ATGCCCCACC AGTGGTTGGA AGGAAACCTA CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC 
AAGACCTGTG GCAGTGTGCT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CGTGTAAAGA ATCCTTGCTG ACCAAGTGCC CACTTGGCCT GTGCAAAGTG 
TCAGTCATCC CACCCACGGC TCTCAACAGC ATCGACTCCG ATGGGTTCTG GAAGGCCAGC 
TGTCCTCCTT CTTGCACAAG CCCACTGTTG GTCTTCGTCA ATTCAAAAAG TGGGGACAAC 
CAGGGTGTGA AGTTCCTCAG AAGATTCAAA CAGCTACTAA ACCCCGCCCA GGTCTTCGAC 
CTCATGAACG GAGGCCCACA CCTCGGCTTA CGGTTATTCC AGAAGTTTGA CACATTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT GGCTGGGTCC TCTCCGAAAT CGACAGCCTC 
AACCTTCATA AACAGTGTCA GCTGGGAGTG CTGCCGCTCG GCACAGGGAA CGACTTGGCC 
CGAGTACTGG GCTGGGGCTC AGCCTGCGAT GACGACACCC AGCTCCCCCA GATCTTGGAG 
AAGTTGGAGA GAGCCAGCAC CAAGATGCTG GACAGGTGGA GCGTCATGGC ATACGAGGCC 
AAGCTCCCCC GGCAGGCCTC CTCCTCTACC GTCACCGAAG ACTTCAGCGA GGATTCCGAG 
GTACAGCAGA TTCTCTTCTA TGAAGACTCG GTTGCAGCCC ACCTTTCTAA AATCCTCACC 
TCGGACCAGC ACTCGGTGGT CATCTCCTCG GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC 
TTCGTGGCAC GGGTGGGGAA GGCCTATGAG AAGACGACCG AGAGCTCGGA GGAGTCAGAG 
GTCATGGCCA AGAAGTGCTC TGTCCTGAAA GAGAAGCTGG ATTCCCTTCT CAAGACCTTG 
GACGATGAGT CCCAGGCCTC GTCCTCTCTG CCCAACCCGC CCCCCACCAT TGCCGAGGAG 
GCTGAAGATG GAGATGGGTC GGGCAGCATC TGCGGTTCCA CCGGAGACCG CTTGGTGGCA 
TCAGCTTGCC CGGCCCGGCC GCAGATATTC CGGCCTCGAG AACAGCTCAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGTCAGATC ATAGAACACA CAGAAAAAGC TGTCGATGAG 
CAGAATGCCC AGACCCAGGA GCAGGAGGGC TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG 
AAGATGGACC ACAGAGTGTG CCCACCACTG TCCCACAGCG AGAGCTTCGG GGTCCCCAAG 
GGGAGGAGCC AGCGCAAAGT GTCGAAATCT CCGTGTGAAA AGCTGATCAG CAAAGGGAGT 
CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT 
GCGCTCAACA CCAAGATCCT GTACCCAAAT GTCCGGGCTG GAATGTCTGG TTCCTTACCC 
GGTGGCTCAG TCATCAGTCG CCTGTTAATT AATGCTGATC CCTTCAACTC TGAACCAGAA 
ACCCTAGAGT ATTACACGGA GAAATGTGTC ATGAACAACT ATTTTGGCAT TGGCCTGGAT 
GCGAAGATAT CCCTGGACTT TAACAACAAG CGCGATGAGC ACCCAGAGAA GTGCAGGAGC 
CGAACCAAGA ACATGATGTG GTATGGAGTT CTTGGAACCA AAGAGTTGCT GCACAGAACC 
TACAAGAACC TGGAGCAAAA GGTCTTGCTG GAGTGTGACG GGCGACCCAT CCCACTCCCC 
AGTCTTCAGG GAATTGCTGT CCTTAACATT CCCAGCTATG CCGGAGGAAC CAACTTCTGG 
GGGGGTACCA AGGAAGATGA TACTTTCGCA GCTCCATCAT TCGATGACAA GATTCTGGAG 
GTGGTCGCCG TGTTCGGCAG CATGCAGATG GCCGTCTCTC GAGTCATCAG GCTACAGCAT 
CATCGGATCG CCCAGTGTCG CACGGTGAAG ATCTCCATCC TTGGGGATGA GGGCGTGCCT 
GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG CCGCCAGGGT ACATTCGGAT TGTCCACAAG 
AACCGGGCAC AGACACTGAC CAGAGACAGG GCATTTGAGA GCACCCTGAA GTCCTGGGAA 
GACAAGCAGA AGTGCGAGCT GCCCCGCCCT CCATCCTGTT CCCTGCACCC GGAGATGCTG 
TCCGAGGAGG AGGCCACCCA GATGGACCAG TTTGGGCAGG CAGCAGGGGT CCTCATTCAC 
AGTATCCGAG AAATAGCTCA GTCTCACCGG GACATGGAGC AGGAACTGGC CCACGCCGTC 
AATGCCAGCT CCAAGTCCAT GGACCGTGTG TATGGCAAGC CCAGAACCAC AGAGGGGCTC 
AACTGCAGCT TCGTCCTGGA AATGGTGAAT AACTTCAGAG CTCTGCGCAG TGAGACGGAG 
CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG CTGGATCCGC CTCAGAAGGA GCAGCTGGGG 
AGTGCTCTTG CCGAGATGGA CCGACAGCTC AGGAGGCTGG CAGACACCCC GTGGCTCTGC 
CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT GTGATGCTGG ATCTTGCCAA GCGCAGTCGC 
AGTGGTAAAT TCCGCCTCGT GACCAAGTTT AAAAAGGAGA AAAACAACAA GAACAAAGAA 
GCTCACAGTA GCCTGGGAGC CCCGGTTCAC CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG 
CTGGAGCACC TCAGTCTCTG TGAGTATAAG GACATCTTCA CACGGCACGA CATCCGGGGC 
TCTGAGCTCC TGCACCTGGA GCGGAGGGAC CTCAAGGACC TGGGCGTGAC CAAGGTGGGC 
CACATGAAGA GGATCCTGTG TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246165.1
CDS	233..3478
Translation	MEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_005246108.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246108.1

ORF Insert Sequence:

ATGGAAGATT GGATTGCAGC ATTAAAGACT GTGCAGAACA GGGAGCACTT TGAGCCCACC 
CAGTACAGCA TGGACCACTT CTCAGGGATG CACAATTGGT ACGCCTGTTC CCACGCGAGG 
CCGACCTACT GCAATGTGTG CCGTGAGGCT CTGTCTGGGG TCACGTCGCA CGGGCTGTCC 
TGCGAGGTGT GCAAATTTAA GGCCCACAAG CGCTGTGCTG TGCGTGCAAC CAATAACTGC 
AAGTGGACCA CACTGGCCTC GATCGGGAAG GACATCATTG AAGATGCAGA TGGGATTGCA 
ATGCCCCACC AGTGGTTGGA AGGAAACCTA CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC 
AAGACCTGTG GCAGTGTGCT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CGTGTAAAGA ATCCTTGCTG ACCAAGTGCC CACTTGGCCT GTGCAAAGTG 
TCAGTCATCC CACCCACGGC TCTCAACAGC ATCGACTCCG ATGGGTTCTG GAAGGCCAGC 
TGTCCTCCTT CTTGCACAAG CCCACTGTTG GTCTTCGTCA ATTCAAAAAG TGGGGACAAC 
CAGGGTGTGA AGTTCCTCAG AAGATTCAAA CAGCTACTAA ACCCCGCCCA GGTCTTCGAC 
CTCATGAACG GAGGCCCACA CCTCGGCTTA CGGTTATTCC AGAAGTTTGA CACATTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT GGCTGGGTCC TCTCCGAAAT CGACAGCCTC 
AACCTTCATA AACAGTGTCA GCTGGGAGTG CTGCCGCTCG GCACAGGGAA CGACTTGGCC 
CGAGTACTGG GCTGGGGCTC AGCCTGCGAT GACGACACCC AGCTCCCCCA GATCTTGGAG 
AAGTTGGAGA GAGCCAGCAC CAAGATGCTG GACAGGTGGA GCGTCATGGC ATACGAGGCC 
AAGCTCCCCC GGCAGGCCTC CTCCTCTACC GTCACCGAAG ACTTCAGCGA GGATTCCGAG 
GTACAGCAGA TTCTCTTCTA TGAAGACTCG GTTGCAGCCC ACCTTTCTAA AATCCTCACC 
TCGGACCAGC ACTCGGTGGT CATCTCCTCG GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC 
TTCGTGGCAC GGGTGGGGAA GGCCTATGAG AAGACGACCG AGAGCTCGGA GGAGTCAGAG 
GTCATGGCCA AGAAGTGCTC TGTCCTGAAA GAGAAGCTGG ATTCCCTTCT CAAGACCTTG 
GACGATGAGT CCCAGGCCTC GTCCTCTCTG CCCAACCCGC CCCCCACCAT TGCCGAGGAG 
GCTGAAGATG GAGATGGGTC GGGCAGCATC TGCGGTTCCA CCGGAGACCG CTTGGTGGCA 
TCAGCTTGCC CGGCCCGGCC GCAGATATTC CGGCCTCGAG AACAGCTCAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGTCAGATC ATAGAACACA CAGAAAAAGC TGTCGATGAG 
CAGAATGCCC AGACCCAGGA GCAGGAGGGC TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG 
AAGATGGACC ACAGAGTGTG CCCACCACTG TCCCACAGCG AGAGCTTCGG GGTCCCCAAG 
GGGAGGAGCC AGCGCAAAGT GTCGAAATCT CCGTGTGAAA AGCTGATCAG CAAAGGGAGT 
CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT 
GCGCTCAACA CCAAGATCCT GTACCCAAAT GTCCGGGCTG GAATGTCTGG TTCCTTACCC 
GGTGGCTCAG TCATCAGTCG CCTGTTAATT AATGCTGATC CCTTCAACTC TGAACCAGAA 
ACCCTAGAGT ATTACACGGA GAAATGTGTC ATGAACAACT ATTTTGGCAT TGGCCTGGAT 
GCGAAGATAT CCCTGGACTT TAACAACAAG CGCGATGAGC ACCCAGAGAA GTGCAGGAGC 
CGAACCAAGA ACATGATGTG GTATGGAGTT CTTGGAACCA AAGAGTTGCT GCACAGAACC 
TACAAGAACC TGGAGCAAAA GGTCTTGCTG GAGTGTGACG GGCGACCCAT CCCACTCCCC 
AGTCTTCAGG GAATTGCTGT CCTTAACATT CCCAGCTATG CCGGAGGAAC CAACTTCTGG 
GGGGGTACCA AGGAAGATGA TACTTTCGCA GCTCCATCAT TCGATGACAA GATTCTGGAG 
GTGGTCGCCG TGTTCGGCAG CATGCAGATG GCCGTCTCTC GAGTCATCAG GCTACAGCAT 
CATCGGATCG CCCAGTGTCG CACGGTGAAG ATCTCCATCC TTGGGGATGA GGGCGTGCCT 
GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG CCGCCAGGGT ACATTCGGAT TGTCCACAAG 
AACCGGGCAC AGACACTGAC CAGAGACAGG GCATTTGAGA GCACCCTGAA GTCCTGGGAA 
GACAAGCAGA AGTGCGAGCT GCCCCGCCCT CCATCCTGTT CCCTGCACCC GGAGATGCTG 
TCCGAGGAGG AGGCCACCCA GATGGACCAG TTTGGGCAGG CAGCAGGGGT CCTCATTCAC 
AGTATCCGAG AAATAGCTCA GTCTCACCGG GACATGGAGC AGGAACTGGC CCACGCCGTC 
AATGCCAGCT CCAAGTCCAT GGACCGTGTG TATGGCAAGC CCAGAACCAC AGAGGGGCTC 
AACTGCAGCT TCGTCCTGGA AATGGTGAAT AACTTCAGAG CTCTGCGCAG TGAGACGGAG 
CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG CTGGATCCGC CTCAGAAGGA GCAGCTGGGG 
AGTGCTCTTG CCGAGATGGA CCGACAGCTC AGGAGGCTGG CAGACACCCC GTGGCTCTGC 
CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT GTGATGCTGG ATCTTGCCAA GCGCAGTCGC 
AGTGGTAAAT TCCGCCTCGT GACCAAGTTT AAAAAGGAGA AAAACAACAA GAACAAAGAA 
GCTCACAGTA GCCTGGGAGC CCCGGTTCAC CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG 
CTGGAGCACC TCAGTCTCTG TGAGTATAAG GACATCTTCA CACGGCACGA CATCCGGGGC 
TCTGAGCTCC TGCACCTGGA GCGGAGGGAC CTCAAGGACC TGGGCGTGAC CAAGGTGGGC 
CACATGAAGA GGATCCTGTG TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG 
GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512028.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510330.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu61923
Clone ID Related Accession (Same CDS sequence)	XM_011512028.3 , XM_011512028.2
Accession Version	XM_011512028.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512028.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTTC AGACTCCAGG GACAGCTGTC ACCCACCAAA AACTGGGGTC ACAAGGGGTC 
CATCTGGCAG TGGCTGGAGC CTTGCAAGGG CACAGCTGCT GCTGCGGATG TACTGTTCCC 
CTCCTGCCTG GGAACCCTGG CCTTGCTGGG GTCACCTCCT CCACAACTTG GACTGGAGCA 
GAAGGACAAA GAATGGATCT GAGGACAAGC TGGCCCAGGA CCAGAACTAC CATCATCAAA 
GAGGGGATGC TGACCAAACA GAACAATTCA TTCCAGCGAT CAAAAAGGAG ATACTTTAAG 
CTTCGAGGGC GAACGCTTTA CTATGCCAAA ACGGCAAAGT CAATCATATT TGATGAGGTG 
GATCTGACAG ATGCCAGCGT AGCTGAATCC AGTACCAAAA ACGTCAACAA CAGTTTTACG 
GTCATAACTC CATGCAGGAA GCTCATCTTG TGTGCTGATA ACAGAAAAGA AATGGAAGAT 
TGGATTGCAG CATTAAAGAC TGTGCAGAAC AGGGAGCACT TTGAGCCCAC CCAGTACAGC 
ATGGACCACT TCTCAGGGAT GCACAATTGG TACGCCTGTT CCCACGCGAG GCCGACCTAC 
TGCAATGTGT GCCGTGAGGC TCTGTCTGGG GTCACGTCGC ACGGGCTGTC CTGCGAGGTG 
TGCAAATTTA AGGCCCACAA GCGCTGTGCT GTGCGTGCAA CCAATAACTG CAAGTGGACC 
ACACTGGCCT CGATCGGGAA GGACATCATT GAAGATGCAG ATGGGATTGC AATGCCCCAC 
CAGTGGTTGG AAGGAAACCT ACCTGTGAGC GCCAAGTGCA CTGTGTGCGA CAAGACCTGT 
GGCAGTGTGC TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCGTGTAAAG AATCCTTGCT GACCAAGTGC CCACTTGGCC TGTGCAAAGT GTCAGTCATC 
CCACCCACGG CTCTCAACAG CATCGACTCC GATGGGTTCT GGAAGGCCAG CTGTCCTCCT 
TCTTGCACAA GCCCACTGTT GGTCTTCGTC AATTCAAAAA GTGGGGACAA CCAGGGTGTG 
AAGTTCCTCA GAAGATTCAA ACAGCTACTA AACCCCGCCC AGGTCTTCGA CCTCATGAAC 
GGAGGCCCAC ACCTCGGCTT ACGGTTATTC CAGAAGTTTG ACACATTCCG GATTCTGGTT 
TGTGGCGGGG ATGGAAGTGT TGGCTGGGTC CTCTCCGAAA TCGACAGCCT CAACCTTCAT 
AAACAGTGTC AGCTGGGAGT GCTGCCGCTC GGCACAGGGA ACGACTTGGC CCGAGTACTG 
GGCTGGGGCT CAGCCTGCGA TGACGACACC CAGCTCCCCC AGATCTTGGA GAAGTTGGAG 
AGAGCCAGCA CCAAGATGCT GGACAGGTGG AGCGTCATGG CATACGAGGC CAAGCTCCCC 
CGGCAGGCCT CCTCCTCTAC CGTCACCGAA GACTTCAGCG AGGATTCCGA GGTACAGCAG 
ATTCTCTTCT ATGAAGACTC GGTTGCAGCC CACCTTTCTA AAATCCTCAC CTCGGACCAG 
CACTCGGTGG TCATCTCCTC GGCCAAAGTG CTCTGTGAGA CGGTGAAGGA CTTCGTGGCA 
CGGGTGGGGA AGGCCTATGA GAAGACGACC GAGAGCTCGG AGGAGTCAGA GGTCATGGCC 
AAGAAGTGCT CTGTCCTGAA AGAGAAGCTG GATTCCCTTC TCAAGACCTT GGACGATGAG 
TCCCAGGCCT CGTCCTCTCT GCCCAACCCG CCCCCCACCA TTGCCGAGGA GGCTGAAGAT 
GGAGATGGGT CGGGCAGCAT CTGCGGTTCC ACCGGAGACC GCTTGGTGGC ATCAGCTTGC 
CCGGCCCGGC CGCAGATATT CCGGCCTCGA GAACAGCTCA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGTCAGAT CATAGAACAC ACAGAAAAAG CTGTCGATGA GCAGAATGCC 
CAGACCCAGG AGCAGGAGGG CTTCGTCCTG GGCCTCTCTG AGTCAGAGGA GAAGATGGAC 
CACAGAGTGT GCCCACCACT GTCCCACAGC GAGAGCTTCG GGGTCCCCAA GGGGAGGAGC 
CAGCGCAAAG TGTCGAAATC TCCGTGTGAA AAGCTGATCA GCAAAGGGAG TCTGTCCCTA 
GGCAGTTCTG CTTCCCTTCC GCCCCAGCCG GGAAGCCGGG ACGGCCTGCC TGCGCTCAAC 
ACCAAGATCC TGTACCCAAA TGTCCGGGCT GGAATGTCTG GTTCCTTACC CGGTGGCTCA 
GTCATCAGTC GCCTGTTAAT TAATGCTGAT CCCTTCAACT CTGAACCAGA AACCCTAGAG 
TATTACACGG AGAAATGTGT CATGAACAAC TATTTTGGCA TTGGCCTGGA TGCGAAGATA 
TCCCTGGACT TTAACAACAA GCGCGATGAG CACCCAGAGA AGTGCAGGAG CCGAACCAAG 
AACATGATGT GGTATGGAGT TCTTGGAACC AAAGAGTTGC TGCACAGAAC CTACAAGAAC 
CTGGAGCAAA AGGTCTTGCT GGAGTGTGAC GGGCGACCCA TCCCACTCCC CAGTCTTCAG 
GGAATTGCTG TCCTTAACAT TCCCAGCTAT GCCGGAGGAA CCAACTTCTG GGGGGGTACC 
AAGGAAGATG ATACTTTCGC AGCTCCATCA TTCGATGACA AGATTCTGGA GGTGGTCGCC 
GTGTTCGGCA GCATGCAGAT GGCCGTCTCT CGAGTCATCA GGCTACAGCA TCATCGGATC 
GCCCAGTGTC GCACGGTGAA GATCTCCATC CTTGGGGATG AGGGCGTGCC TGTGCAGGTG 
GACGGAGAGG CCTGGGTCCA GCCGCCAGGG TACATTCGGA TTGTCCACAA GAACCGGGCA 
CAGACACTGA CCAGAGACAG GGCATTTGAG AGCACCCTGA AGTCCTGGGA AGACAAGCAG 
AAGTGCGAGC TGCCCCGCCC TCCATCCTGT TCCCTGCACC CGGAGATGCT GTCCGAGGAG 
GAGGCCACCC AGATGGACCA GTTTGGGCAG GCAGCAGGGG TCCTCATTCA CAGTATCCGA 
GAAATAGCTC AGTCTCACCG GGACATGGAG CAGGAACTGG CCCACGCCGT CAATGCCAGC 
TCCAAGTCCA TGGACCGTGT GTATGGCAAG CCCAGAACCA CAGAGGGGCT CAACTGCAGC 
TTCGTCCTGG AAATGGTGAA TAACTTCAGA GCTCTGCGCA GTGAGACGGA GCTGCTGCTG 
TCTGGGAAGA TGGCCCTGCA GCTGGATCCG CCTCAGAAGG AGCAGCTGGG GAGTGCTCTT 
GCCGAGATGG ACCGACAGCT CAGGAGGCTG GCAGACACCC CGTGGCTCTG CCAGTCCGCA 
GAGCCCGGCG ACGAAGAGAG TGTGATGCTG GATCTTGCCA AGCGCAGTCG CAGTGGTAAA 
TTCCGCCTCG TGACCAAGTT TAAAAAGGAG AAAAACAACA AGAACAAAGA AGCTCACAGT 
AGCCTGGGAG CCCCGGTTCA CCTCTGGGGG ACAGAGGAGG TTGCTGCCTG GCTGGAGCAC 
CTCAGTCTCT GTGAGTATAA GGACATCTTC ACACGGCACG ACATCCGGGG CTCTGAGCTC 
CTGCACCTGG AGCGGAGGGA CCTCAAGGAC CTGGGCGTGA CCAAGGTGGG CCACATGAAG 
AGGATCCTGT GTGGCATCAA GGAGCTGAGC CGCAGCGCCC CCGCCGTCGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510330.1
CDS	1401..5117
Translation	MAFQTPGTAVTHQKLGSQGVHLAVAGALQGHSCCCGCTVPLLPGNPGLAGVTSSTTWTGAEGQRMDLRTSWPRTRTTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_011512028.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512028.3

ORF Insert Sequence:

ATGGCATTTC AGACTCCAGG GACAGCTGTC ACCCACCAAA AACTGGGGTC ACAAGGGGTC 
CATCTGGCAG TGGCTGGAGC CTTGCAAGGG CACAGCTGCT GCTGCGGATG TACTGTTCCC 
CTCCTGCCTG GGAACCCTGG CCTTGCTGGG GTCACCTCCT CCACAACTTG GACTGGAGCA 
GAAGGACAAA GAATGGATCT GAGGACAAGC TGGCCCAGGA CCAGAACTAC CATCATCAAA 
GAGGGGATGC TGACCAAACA GAACAATTCA TTCCAGCGAT CAAAAAGGAG ATACTTTAAG 
CTTCGAGGGC GAACGCTTTA CTATGCCAAA ACGGCAAAGT CAATCATATT TGATGAGGTG 
GATCTGACAG ATGCCAGCGT AGCTGAATCC AGTACCAAAA ACGTCAACAA CAGTTTTACG 
GTCATAACTC CATGCAGGAA GCTCATCTTG TGTGCTGATA ACAGAAAAGA AATGGAAGAT 
TGGATTGCAG CATTAAAGAC TGTGCAGAAC AGGGAGCACT TTGAGCCCAC CCAGTACAGC 
ATGGACCACT TCTCAGGGAT GCACAATTGG TACGCCTGTT CCCACGCGAG GCCGACCTAC 
TGCAATGTGT GCCGTGAGGC TCTGTCTGGG GTCACGTCGC ACGGGCTGTC CTGCGAGGTG 
TGCAAATTTA AGGCCCACAA GCGCTGTGCT GTGCGTGCAA CCAATAACTG CAAGTGGACC 
ACACTGGCCT CGATCGGGAA GGACATCATT GAAGATGCAG ATGGGATTGC AATGCCCCAC 
CAGTGGTTGG AAGGAAACCT ACCTGTGAGC GCCAAGTGCA CTGTGTGCGA CAAGACCTGT 
GGCAGTGTGC TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCGTGTAAAG AATCCTTGCT GACCAAGTGC CCACTTGGCC TGTGCAAAGT GTCAGTCATC 
CCACCCACGG CTCTCAACAG CATCGACTCC GATGGGTTCT GGAAGGCCAG CTGTCCTCCT 
TCTTGCACAA GCCCACTGTT GGTCTTCGTC AATTCAAAAA GTGGGGACAA CCAGGGTGTG 
AAGTTCCTCA GAAGATTCAA ACAGCTACTA AACCCCGCCC AGGTCTTCGA CCTCATGAAC 
GGAGGCCCAC ACCTCGGCTT ACGGTTATTC CAGAAGTTTG ACACATTCCG GATTCTGGTT 
TGTGGCGGGG ATGGAAGTGT TGGCTGGGTC CTCTCCGAAA TCGACAGCCT CAACCTTCAT 
AAACAGTGTC AGCTGGGAGT GCTGCCGCTC GGCACAGGGA ACGACTTGGC CCGAGTACTG 
GGCTGGGGCT CAGCCTGCGA TGACGACACC CAGCTCCCCC AGATCTTGGA GAAGTTGGAG 
AGAGCCAGCA CCAAGATGCT GGACAGGTGG AGCGTCATGG CATACGAGGC CAAGCTCCCC 
CGGCAGGCCT CCTCCTCTAC CGTCACCGAA GACTTCAGCG AGGATTCCGA GGTACAGCAG 
ATTCTCTTCT ATGAAGACTC GGTTGCAGCC CACCTTTCTA AAATCCTCAC CTCGGACCAG 
CACTCGGTGG TCATCTCCTC GGCCAAAGTG CTCTGTGAGA CGGTGAAGGA CTTCGTGGCA 
CGGGTGGGGA AGGCCTATGA GAAGACGACC GAGAGCTCGG AGGAGTCAGA GGTCATGGCC 
AAGAAGTGCT CTGTCCTGAA AGAGAAGCTG GATTCCCTTC TCAAGACCTT GGACGATGAG 
TCCCAGGCCT CGTCCTCTCT GCCCAACCCG CCCCCCACCA TTGCCGAGGA GGCTGAAGAT 
GGAGATGGGT CGGGCAGCAT CTGCGGTTCC ACCGGAGACC GCTTGGTGGC ATCAGCTTGC 
CCGGCCCGGC CGCAGATATT CCGGCCTCGA GAACAGCTCA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGTCAGAT CATAGAACAC ACAGAAAAAG CTGTCGATGA GCAGAATGCC 
CAGACCCAGG AGCAGGAGGG CTTCGTCCTG GGCCTCTCTG AGTCAGAGGA GAAGATGGAC 
CACAGAGTGT GCCCACCACT GTCCCACAGC GAGAGCTTCG GGGTCCCCAA GGGGAGGAGC 
CAGCGCAAAG TGTCGAAATC TCCGTGTGAA AAGCTGATCA GCAAAGGGAG TCTGTCCCTA 
GGCAGTTCTG CTTCCCTTCC GCCCCAGCCG GGAAGCCGGG ACGGCCTGCC TGCGCTCAAC 
ACCAAGATCC TGTACCCAAA TGTCCGGGCT GGAATGTCTG GTTCCTTACC CGGTGGCTCA 
GTCATCAGTC GCCTGTTAAT TAATGCTGAT CCCTTCAACT CTGAACCAGA AACCCTAGAG 
TATTACACGG AGAAATGTGT CATGAACAAC TATTTTGGCA TTGGCCTGGA TGCGAAGATA 
TCCCTGGACT TTAACAACAA GCGCGATGAG CACCCAGAGA AGTGCAGGAG CCGAACCAAG 
AACATGATGT GGTATGGAGT TCTTGGAACC AAAGAGTTGC TGCACAGAAC CTACAAGAAC 
CTGGAGCAAA AGGTCTTGCT GGAGTGTGAC GGGCGACCCA TCCCACTCCC CAGTCTTCAG 
GGAATTGCTG TCCTTAACAT TCCCAGCTAT GCCGGAGGAA CCAACTTCTG GGGGGGTACC 
AAGGAAGATG ATACTTTCGC AGCTCCATCA TTCGATGACA AGATTCTGGA GGTGGTCGCC 
GTGTTCGGCA GCATGCAGAT GGCCGTCTCT CGAGTCATCA GGCTACAGCA TCATCGGATC 
GCCCAGTGTC GCACGGTGAA GATCTCCATC CTTGGGGATG AGGGCGTGCC TGTGCAGGTG 
GACGGAGAGG CCTGGGTCCA GCCGCCAGGG TACATTCGGA TTGTCCACAA GAACCGGGCA 
CAGACACTGA CCAGAGACAG GGCATTTGAG AGCACCCTGA AGTCCTGGGA AGACAAGCAG 
AAGTGCGAGC TGCCCCGCCC TCCATCCTGT TCCCTGCACC CGGAGATGCT GTCCGAGGAG 
GAGGCCACCC AGATGGACCA GTTTGGGCAG GCAGCAGGGG TCCTCATTCA CAGTATCCGA 
GAAATAGCTC AGTCTCACCG GGACATGGAG CAGGAACTGG CCCACGCCGT CAATGCCAGC 
TCCAAGTCCA TGGACCGTGT GTATGGCAAG CCCAGAACCA CAGAGGGGCT CAACTGCAGC 
TTCGTCCTGG AAATGGTGAA TAACTTCAGA GCTCTGCGCA GTGAGACGGA GCTGCTGCTG 
TCTGGGAAGA TGGCCCTGCA GCTGGATCCG CCTCAGAAGG AGCAGCTGGG GAGTGCTCTT 
GCCGAGATGG ACCGACAGCT CAGGAGGCTG GCAGACACCC CGTGGCTCTG CCAGTCCGCA 
GAGCCCGGCG ACGAAGAGAG TGTGATGCTG GATCTTGCCA AGCGCAGTCG CAGTGGTAAA 
TTCCGCCTCG TGACCAAGTT TAAAAAGGAG AAAAACAACA AGAACAAAGA AGCTCACAGT 
AGCCTGGGAG CCCCGGTTCA CCTCTGGGGG ACAGAGGAGG TTGCTGCCTG GCTGGAGCAC 
CTCAGTCTCT GTGAGTATAA GGACATCTTC ACACGGCACG ACATCCGGGG CTCTGAGCTC 
CTGCACCTGG AGCGGAGGGA CCTCAAGGAC CTGGGCGTGA CCAAGGTGGG CCACATGAAG 
AGGATCCTGT GTGGCATCAA GGAGCTGAGC CGCAGCGCCC CCGCCGTCGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512028.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510330.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X1, mRNA.

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CloneID	OHu61923
Clone ID Related Accession (Same CDS sequence)	XM_011512028.3 , XM_011512028.2
Accession Version	XM_011512028.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512028.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTTC AGACTCCAGG GACAGCTGTC ACCCACCAAA AACTGGGGTC ACAAGGGGTC 
CATCTGGCAG TGGCTGGAGC CTTGCAAGGG CACAGCTGCT GCTGCGGATG TACTGTTCCC 
CTCCTGCCTG GGAACCCTGG CCTTGCTGGG GTCACCTCCT CCACAACTTG GACTGGAGCA 
GAAGGACAAA GAATGGATCT GAGGACAAGC TGGCCCAGGA CCAGAACTAC CATCATCAAA 
GAGGGGATGC TGACCAAACA GAACAATTCA TTCCAGCGAT CAAAAAGGAG ATACTTTAAG 
CTTCGAGGGC GAACGCTTTA CTATGCCAAA ACGGCAAAGT CAATCATATT TGATGAGGTG 
GATCTGACAG ATGCCAGCGT AGCTGAATCC AGTACCAAAA ACGTCAACAA CAGTTTTACG 
GTCATAACTC CATGCAGGAA GCTCATCTTG TGTGCTGATA ACAGAAAAGA AATGGAAGAT 
TGGATTGCAG CATTAAAGAC TGTGCAGAAC AGGGAGCACT TTGAGCCCAC CCAGTACAGC 
ATGGACCACT TCTCAGGGAT GCACAATTGG TACGCCTGTT CCCACGCGAG GCCGACCTAC 
TGCAATGTGT GCCGTGAGGC TCTGTCTGGG GTCACGTCGC ACGGGCTGTC CTGCGAGGTG 
TGCAAATTTA AGGCCCACAA GCGCTGTGCT GTGCGTGCAA CCAATAACTG CAAGTGGACC 
ACACTGGCCT CGATCGGGAA GGACATCATT GAAGATGCAG ATGGGATTGC AATGCCCCAC 
CAGTGGTTGG AAGGAAACCT ACCTGTGAGC GCCAAGTGCA CTGTGTGCGA CAAGACCTGT 
GGCAGTGTGC TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCGTGTAAAG AATCCTTGCT GACCAAGTGC CCACTTGGCC TGTGCAAAGT GTCAGTCATC 
CCACCCACGG CTCTCAACAG CATCGACTCC GATGGGTTCT GGAAGGCCAG CTGTCCTCCT 
TCTTGCACAA GCCCACTGTT GGTCTTCGTC AATTCAAAAA GTGGGGACAA CCAGGGTGTG 
AAGTTCCTCA GAAGATTCAA ACAGCTACTA AACCCCGCCC AGGTCTTCGA CCTCATGAAC 
GGAGGCCCAC ACCTCGGCTT ACGGTTATTC CAGAAGTTTG ACACATTCCG GATTCTGGTT 
TGTGGCGGGG ATGGAAGTGT TGGCTGGGTC CTCTCCGAAA TCGACAGCCT CAACCTTCAT 
AAACAGTGTC AGCTGGGAGT GCTGCCGCTC GGCACAGGGA ACGACTTGGC CCGAGTACTG 
GGCTGGGGCT CAGCCTGCGA TGACGACACC CAGCTCCCCC AGATCTTGGA GAAGTTGGAG 
AGAGCCAGCA CCAAGATGCT GGACAGGTGG AGCGTCATGG CATACGAGGC CAAGCTCCCC 
CGGCAGGCCT CCTCCTCTAC CGTCACCGAA GACTTCAGCG AGGATTCCGA GGTACAGCAG 
ATTCTCTTCT ATGAAGACTC GGTTGCAGCC CACCTTTCTA AAATCCTCAC CTCGGACCAG 
CACTCGGTGG TCATCTCCTC GGCCAAAGTG CTCTGTGAGA CGGTGAAGGA CTTCGTGGCA 
CGGGTGGGGA AGGCCTATGA GAAGACGACC GAGAGCTCGG AGGAGTCAGA GGTCATGGCC 
AAGAAGTGCT CTGTCCTGAA AGAGAAGCTG GATTCCCTTC TCAAGACCTT GGACGATGAG 
TCCCAGGCCT CGTCCTCTCT GCCCAACCCG CCCCCCACCA TTGCCGAGGA GGCTGAAGAT 
GGAGATGGGT CGGGCAGCAT CTGCGGTTCC ACCGGAGACC GCTTGGTGGC ATCAGCTTGC 
CCGGCCCGGC CGCAGATATT CCGGCCTCGA GAACAGCTCA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGTCAGAT CATAGAACAC ACAGAAAAAG CTGTCGATGA GCAGAATGCC 
CAGACCCAGG AGCAGGAGGG CTTCGTCCTG GGCCTCTCTG AGTCAGAGGA GAAGATGGAC 
CACAGAGTGT GCCCACCACT GTCCCACAGC GAGAGCTTCG GGGTCCCCAA GGGGAGGAGC 
CAGCGCAAAG TGTCGAAATC TCCGTGTGAA AAGCTGATCA GCAAAGGGAG TCTGTCCCTA 
GGCAGTTCTG CTTCCCTTCC GCCCCAGCCG GGAAGCCGGG ACGGCCTGCC TGCGCTCAAC 
ACCAAGATCC TGTACCCAAA TGTCCGGGCT GGAATGTCTG GTTCCTTACC CGGTGGCTCA 
GTCATCAGTC GCCTGTTAAT TAATGCTGAT CCCTTCAACT CTGAACCAGA AACCCTAGAG 
TATTACACGG AGAAATGTGT CATGAACAAC TATTTTGGCA TTGGCCTGGA TGCGAAGATA 
TCCCTGGACT TTAACAACAA GCGCGATGAG CACCCAGAGA AGTGCAGGAG CCGAACCAAG 
AACATGATGT GGTATGGAGT TCTTGGAACC AAAGAGTTGC TGCACAGAAC CTACAAGAAC 
CTGGAGCAAA AGGTCTTGCT GGAGTGTGAC GGGCGACCCA TCCCACTCCC CAGTCTTCAG 
GGAATTGCTG TCCTTAACAT TCCCAGCTAT GCCGGAGGAA CCAACTTCTG GGGGGGTACC 
AAGGAAGATG ATACTTTCGC AGCTCCATCA TTCGATGACA AGATTCTGGA GGTGGTCGCC 
GTGTTCGGCA GCATGCAGAT GGCCGTCTCT CGAGTCATCA GGCTACAGCA TCATCGGATC 
GCCCAGTGTC GCACGGTGAA GATCTCCATC CTTGGGGATG AGGGCGTGCC TGTGCAGGTG 
GACGGAGAGG CCTGGGTCCA GCCGCCAGGG TACATTCGGA TTGTCCACAA GAACCGGGCA 
CAGACACTGA CCAGAGACAG GGCATTTGAG AGCACCCTGA AGTCCTGGGA AGACAAGCAG 
AAGTGCGAGC TGCCCCGCCC TCCATCCTGT TCCCTGCACC CGGAGATGCT GTCCGAGGAG 
GAGGCCACCC AGATGGACCA GTTTGGGCAG GCAGCAGGGG TCCTCATTCA CAGTATCCGA 
GAAATAGCTC AGTCTCACCG GGACATGGAG CAGGAACTGG CCCACGCCGT CAATGCCAGC 
TCCAAGTCCA TGGACCGTGT GTATGGCAAG CCCAGAACCA CAGAGGGGCT CAACTGCAGC 
TTCGTCCTGG AAATGGTGAA TAACTTCAGA GCTCTGCGCA GTGAGACGGA GCTGCTGCTG 
TCTGGGAAGA TGGCCCTGCA GCTGGATCCG CCTCAGAAGG AGCAGCTGGG GAGTGCTCTT 
GCCGAGATGG ACCGACAGCT CAGGAGGCTG GCAGACACCC CGTGGCTCTG CCAGTCCGCA 
GAGCCCGGCG ACGAAGAGAG TGTGATGCTG GATCTTGCCA AGCGCAGTCG CAGTGGTAAA 
TTCCGCCTCG TGACCAAGTT TAAAAAGGAG AAAAACAACA AGAACAAAGA AGCTCACAGT 
AGCCTGGGAG CCCCGGTTCA CCTCTGGGGG ACAGAGGAGG TTGCTGCCTG GCTGGAGCAC 
CTCAGTCTCT GTGAGTATAA GGACATCTTC ACACGGCACG ACATCCGGGG CTCTGAGCTC 
CTGCACCTGG AGCGGAGGGA CCTCAAGGAC CTGGGCGTGA CCAAGGTGGG CCACATGAAG 
AGGATCCTGT GTGGCATCAA GGAGCTGAGC CGCAGCGCCC CCGCCGTCGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510330.1
CDS	1400..5116
Translation	MAFQTPGTAVTHQKLGSQGVHLAVAGALQGHSCCCGCTVPLLPGNPGLAGVTSSTTWTGAEGQRMDLRTSWPRTRTTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_011512028.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512028.2

ORF Insert Sequence:

ATGGCATTTC AGACTCCAGG GACAGCTGTC ACCCACCAAA AACTGGGGTC ACAAGGGGTC 
CATCTGGCAG TGGCTGGAGC CTTGCAAGGG CACAGCTGCT GCTGCGGATG TACTGTTCCC 
CTCCTGCCTG GGAACCCTGG CCTTGCTGGG GTCACCTCCT CCACAACTTG GACTGGAGCA 
GAAGGACAAA GAATGGATCT GAGGACAAGC TGGCCCAGGA CCAGAACTAC CATCATCAAA 
GAGGGGATGC TGACCAAACA GAACAATTCA TTCCAGCGAT CAAAAAGGAG ATACTTTAAG 
CTTCGAGGGC GAACGCTTTA CTATGCCAAA ACGGCAAAGT CAATCATATT TGATGAGGTG 
GATCTGACAG ATGCCAGCGT AGCTGAATCC AGTACCAAAA ACGTCAACAA CAGTTTTACG 
GTCATAACTC CATGCAGGAA GCTCATCTTG TGTGCTGATA ACAGAAAAGA AATGGAAGAT 
TGGATTGCAG CATTAAAGAC TGTGCAGAAC AGGGAGCACT TTGAGCCCAC CCAGTACAGC 
ATGGACCACT TCTCAGGGAT GCACAATTGG TACGCCTGTT CCCACGCGAG GCCGACCTAC 
TGCAATGTGT GCCGTGAGGC TCTGTCTGGG GTCACGTCGC ACGGGCTGTC CTGCGAGGTG 
TGCAAATTTA AGGCCCACAA GCGCTGTGCT GTGCGTGCAA CCAATAACTG CAAGTGGACC 
ACACTGGCCT CGATCGGGAA GGACATCATT GAAGATGCAG ATGGGATTGC AATGCCCCAC 
CAGTGGTTGG AAGGAAACCT ACCTGTGAGC GCCAAGTGCA CTGTGTGCGA CAAGACCTGT 
GGCAGTGTGC TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCGTGTAAAG AATCCTTGCT GACCAAGTGC CCACTTGGCC TGTGCAAAGT GTCAGTCATC 
CCACCCACGG CTCTCAACAG CATCGACTCC GATGGGTTCT GGAAGGCCAG CTGTCCTCCT 
TCTTGCACAA GCCCACTGTT GGTCTTCGTC AATTCAAAAA GTGGGGACAA CCAGGGTGTG 
AAGTTCCTCA GAAGATTCAA ACAGCTACTA AACCCCGCCC AGGTCTTCGA CCTCATGAAC 
GGAGGCCCAC ACCTCGGCTT ACGGTTATTC CAGAAGTTTG ACACATTCCG GATTCTGGTT 
TGTGGCGGGG ATGGAAGTGT TGGCTGGGTC CTCTCCGAAA TCGACAGCCT CAACCTTCAT 
AAACAGTGTC AGCTGGGAGT GCTGCCGCTC GGCACAGGGA ACGACTTGGC CCGAGTACTG 
GGCTGGGGCT CAGCCTGCGA TGACGACACC CAGCTCCCCC AGATCTTGGA GAAGTTGGAG 
AGAGCCAGCA CCAAGATGCT GGACAGGTGG AGCGTCATGG CATACGAGGC CAAGCTCCCC 
CGGCAGGCCT CCTCCTCTAC CGTCACCGAA GACTTCAGCG AGGATTCCGA GGTACAGCAG 
ATTCTCTTCT ATGAAGACTC GGTTGCAGCC CACCTTTCTA AAATCCTCAC CTCGGACCAG 
CACTCGGTGG TCATCTCCTC GGCCAAAGTG CTCTGTGAGA CGGTGAAGGA CTTCGTGGCA 
CGGGTGGGGA AGGCCTATGA GAAGACGACC GAGAGCTCGG AGGAGTCAGA GGTCATGGCC 
AAGAAGTGCT CTGTCCTGAA AGAGAAGCTG GATTCCCTTC TCAAGACCTT GGACGATGAG 
TCCCAGGCCT CGTCCTCTCT GCCCAACCCG CCCCCCACCA TTGCCGAGGA GGCTGAAGAT 
GGAGATGGGT CGGGCAGCAT CTGCGGTTCC ACCGGAGACC GCTTGGTGGC ATCAGCTTGC 
CCGGCCCGGC CGCAGATATT CCGGCCTCGA GAACAGCTCA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGTCAGAT CATAGAACAC ACAGAAAAAG CTGTCGATGA GCAGAATGCC 
CAGACCCAGG AGCAGGAGGG CTTCGTCCTG GGCCTCTCTG AGTCAGAGGA GAAGATGGAC 
CACAGAGTGT GCCCACCACT GTCCCACAGC GAGAGCTTCG GGGTCCCCAA GGGGAGGAGC 
CAGCGCAAAG TGTCGAAATC TCCGTGTGAA AAGCTGATCA GCAAAGGGAG TCTGTCCCTA 
GGCAGTTCTG CTTCCCTTCC GCCCCAGCCG GGAAGCCGGG ACGGCCTGCC TGCGCTCAAC 
ACCAAGATCC TGTACCCAAA TGTCCGGGCT GGAATGTCTG GTTCCTTACC CGGTGGCTCA 
GTCATCAGTC GCCTGTTAAT TAATGCTGAT CCCTTCAACT CTGAACCAGA AACCCTAGAG 
TATTACACGG AGAAATGTGT CATGAACAAC TATTTTGGCA TTGGCCTGGA TGCGAAGATA 
TCCCTGGACT TTAACAACAA GCGCGATGAG CACCCAGAGA AGTGCAGGAG CCGAACCAAG 
AACATGATGT GGTATGGAGT TCTTGGAACC AAAGAGTTGC TGCACAGAAC CTACAAGAAC 
CTGGAGCAAA AGGTCTTGCT GGAGTGTGAC GGGCGACCCA TCCCACTCCC CAGTCTTCAG 
GGAATTGCTG TCCTTAACAT TCCCAGCTAT GCCGGAGGAA CCAACTTCTG GGGGGGTACC 
AAGGAAGATG ATACTTTCGC AGCTCCATCA TTCGATGACA AGATTCTGGA GGTGGTCGCC 
GTGTTCGGCA GCATGCAGAT GGCCGTCTCT CGAGTCATCA GGCTACAGCA TCATCGGATC 
GCCCAGTGTC GCACGGTGAA GATCTCCATC CTTGGGGATG AGGGCGTGCC TGTGCAGGTG 
GACGGAGAGG CCTGGGTCCA GCCGCCAGGG TACATTCGGA TTGTCCACAA GAACCGGGCA 
CAGACACTGA CCAGAGACAG GGCATTTGAG AGCACCCTGA AGTCCTGGGA AGACAAGCAG 
AAGTGCGAGC TGCCCCGCCC TCCATCCTGT TCCCTGCACC CGGAGATGCT GTCCGAGGAG 
GAGGCCACCC AGATGGACCA GTTTGGGCAG GCAGCAGGGG TCCTCATTCA CAGTATCCGA 
GAAATAGCTC AGTCTCACCG GGACATGGAG CAGGAACTGG CCCACGCCGT CAATGCCAGC 
TCCAAGTCCA TGGACCGTGT GTATGGCAAG CCCAGAACCA CAGAGGGGCT CAACTGCAGC 
TTCGTCCTGG AAATGGTGAA TAACTTCAGA GCTCTGCGCA GTGAGACGGA GCTGCTGCTG 
TCTGGGAAGA TGGCCCTGCA GCTGGATCCG CCTCAGAAGG AGCAGCTGGG GAGTGCTCTT 
GCCGAGATGG ACCGACAGCT CAGGAGGCTG GCAGACACCC CGTGGCTCTG CCAGTCCGCA 
GAGCCCGGCG ACGAAGAGAG TGTGATGCTG GATCTTGCCA AGCGCAGTCG CAGTGGTAAA 
TTCCGCCTCG TGACCAAGTT TAAAAAGGAG AAAAACAACA AGAACAAAGA AGCTCACAGT 
AGCCTGGGAG CCCCGGTTCA CCTCTGGGGG ACAGAGGAGG TTGCTGCCTG GCTGGAGCAC 
CTCAGTCTCT GTGAGTATAA GGACATCTTC ACACGGCACG ACATCCGGGG CTCTGAGCTC 
CTGCACCTGG AGCGGAGGGA CCTCAAGGAC CTGGGCGTGA CCAAGGTGGG CCACATGAAG 
AGGATCCTGT GTGGCATCAA GGAGCTGAGC CGCAGCGCCC CCGCCGTCGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512029.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510331.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X2, mRNA.

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CloneID	OHu61924
Clone ID Related Accession (Same CDS sequence)	XM_011512029.3 , XM_011512029.2
Accession Version	XM_011512029.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTTC AGACTCCAGG GACAGCTGTC ACCCACCAAA AACTGGGGTC ACAAGGGGTC 
CATCTGGCAG TGGCTGGAGC CTTGCAAGGG CACAGCTGCT GCTGCGGATG TACTGTTCCC 
CTCCTGCCTG GGAACCCTGG CCTTGCTGGG GTCACCTCCT CCACAACTTG GACTGGAGCA 
GAAGGACAAA GAATGGATCT GAGGACAAGC TGGCCCAGGA CCAGAACTAC CATCATCAAA 
GAGGGGATGC TGACCAAACA GAACAATTCA TTCCAGCGAT CAAAAAGGAG ATACTTTAAG 
CTTCGAGGGC GAACGCTTTA CTATGCCAAA ACGGCAAAGT CAATCATATT TGATGAGGTG 
GATCTGACAG ATGCCAGCGT AGCTGAATCC AGTACCAAAA ACGTCAACAA CAGTTTTACG 
GTCATAACTC CATGCAGGAA GCTCATCTTG TGTGCTGATA ACAGAAAAGA AATGGAAGAT 
TGGATTGCAG CATTAAAGAC TGTGCAGAAC AGGGAGCACT TTGAGCCCAC CCAGTACAGC 
ATGGACCACT TCTCAGGGAT GCACAATTGG TACGCCTGTT CCCACGCGAG GCCGACCTAC 
TGCAATGTGT GCCGTGAGGC TCTGTCTGGG GTCACGTCGC ACGGGCTGTC CTGCGAGGTG 
TGCAAATTTA AGGCCCACAA GCGCTGTGCT GTGCGTGCAA CCAATAACTG CAAGTGGACC 
ACACTGGCCT CGATCGGGAA GGACATCATT GAAGATGCAG ATGGGATTGC AATGCCCCAC 
CAGTGGTTGG AAGGAAACCT ACCTGTGAGC GCCAAGTGCA CTGTGTGCGA CAAGACCTGT 
GGCAGTGTGC TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCGTGTAAAG AATCCTTGCT GACCAAGTGC CCACTTGGCC TGTGCAAAGT GTCAGTCATC 
CCACCCACGG CTCTCAACAG CATCGACTCC GATGGGTTCT GGAAGGCCAG CTGTCCTCCT 
TCTTGCACAA GCCCACTGTT GGTCTTCGTC AATTCAAAAA GTGGGGACAA CCAGGGTGTG 
AAGTTCCTCA GAAGATTCAA ACAGCTACTA AACCCCGCCC AGGTCTTCGA CCTCATGAAC 
GGAGGCCCAC ACCTCGGCTT ACGGTTATTC CAGAAGTTTG ACACATTCCG GATTCTGGTT 
TGTGGCGGGG ATGGAAGTGT TGGCTGGGTC CTCTCCGAAA TCGACAGCCT CAACCTTCAT 
AAACAGTGTC AGCTGGGAGT GCTGCCGCTC GGCACAGGGA ACGACTTGGC CCGAGTACTG 
GGCTGGGGCT CAGCCTGCGA TGACGACACC CAGCTCCCCC AGATCTTGGA GAAGTTGGAG 
AGAGCCAGCA CCAAGATGCT GGACAGGTGG AGCGTCATGG CATACGAGGC CAAGCTCCCC 
CGGCAGGCCT CCTCCTCTAC CGTCACCGAA GACTTCAGCG AGGATTCCGA GGTACAGCAG 
ATTCTCTTCT ATGAAGACTC GGTTGCAGCC CACCTTTCTA AAATCCTCAC CTCGGACCAG 
CACTCGGTGG TCATCTCCTC GGCCAAAGTG CTCTGTGAGA CGGTGAAGGA CTTCGTGGCA 
CGGGTGGGGA AGGCCTATGA GAAGACGACC GAGAGCTCGG AGGAGTCAGA GGTCATGGCC 
AAGAAGTGCT CTGTCCTGAA AGAGAAGCTG GATTCCCTTC TCAAGACCTT GGACGATGAG 
TCCCAGGCCT CGTCCTCTCT GCCCAACCCG CCCCCCACCA TTGCCGAGGA GGCTGAAGAT 
GGAGATGGGT CGGGCAGCAT CTGCGGTTCC ACCGGAGACC GCTTGGTGGC ATCAGCTTGC 
CCGGCCCGGC CGCAGATATT CCGGCCTCGA GAACAGCTCA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGTCAGAT CATAGAACAC ACAGAAAAAG CTGTCGATGA GCAGAATGCC 
CAGACCCAGG AGCAGGAGGG CTTCGTCCTG GGCCTCTCTG AGTCAGAGGA GAAGATGGAC 
CACAGAGTGT GCCCACCACT GTCCCACAGC GAGAGCTTCG GGGTCCCCAA GGGGAGGAGC 
CAGCGCAAAG TGTCGAAATC TCCGTGTGAA AAGCTGATCA GCAAAGGGAG TCTGTCCCTA 
GGCAGTTCTG CTTCCCTTCC GCCCCAGCCG GGAAGCCGGG ACGGCCTGCC TGCGCTCAAC 
ACCAAGATCC TGTACCCAAA TGTCCGGGCT GGAATGTCTG GTTCCTTACC CGGTGGCTCA 
GTCATCAGTC GCCTGTTAAT TAATGCTGAT CCCTTCAACT CTGAACCAGA AACCCTAGAG 
TATTACACGG AGAAATGTGT CATGAACAAC TATTTTGGCA TTGGCCTGGA TGCGAAGATA 
TCCCTGGACT TTAACAACAA GCGCGATGAG CACCCAGAGA AGTGCAGGAG CCGAACCAAG 
AACATGATGT GGTATGGAGT TCTTGGAACC AAAGAGTTGC TGCACAGAAC CTACAAGAAC 
CTGGAGCAAA AGGTCTTGCT GGAGTGTGAC GGGCGACCCA TCCCACTCCC CAGTCTTCAG 
GGAATTGCTG TCCTTAACAT TCCCAGCTAT GCCGGAGGAA CCAACTTCTG GGGGGGTACC 
AAGGAAGATG ATACTTTCGC AGCTCCATCA TTCGATGACA AGATTCTGGA GGTGGTCGCC 
GTGTTCGGCA GCATGCAGAT GGCCGTCTCT CGAGTCATCA GGCTACAGCA TCATCGGATC 
GCCCAGTGTC GCACGGTGAA GATCTCCATC CTTGGGGATG AGGGCGTGCC TGTGCAGGTG 
GACGGAGAGG CCTGGGTCCA GCCGCCAGGG TACATTCGGA TTGTCCACAA GAACCGGGCA 
CAGACACTGA CCAGAGACAG GGCATTTGAG AGCACCCTGA AGTCCTGGGA AGACAAGCAG 
AAGTGCGAGC TGCCCCGCCC TCCATCCTGT TCCCTGCACC CGGAGATGCT GTCCGAGGAG 
GAGGCCACCC AGATGGACCA GTTTGGGCAG GCAGCAGGGG TCCTCATTCA CAGTATCCGA 
GAAATAGCTC AGTCTCACCG GGACATGGAG CAGGAACTGG CCCACGCCGT CAATGCCAGC 
TCCAAGTCCA TGGACCGTGT GTATGGCAAG CCCAGAACCA CAGAGGGGCT CAACTGCAGC 
TTCGTCCTGG AAATGGTGAA TAACTTCAGA GCTCTGCGCA GTGAGACGGA GCTGCTGCTG 
TCTGGGAAGA TGGCCCTGCA GCTGGATCCG CCTCAGAAGG AGCAGCTGGG GAGTGCTCTT 
GCCGAGATGG ACCGACAGCT CAGGAGGCTG GCAGACACCC CGTGGCTCTG CCAGTCCGCA 
GAGCCCGGCG ACGAAGAGAG TGTGATGCTG GATCTTGCCA AGCGCAGTCG CAGTGGTAAA 
TTCCGCCTCG TGACCAAGTT TAAAAAGGAG AAAAACAACA AGAACAAAGA AGCTCACAGT 
AGCCTGGGAG CCCCGGGACC TGGGCGTGAC CAAGGTGGGC CACATGAAGA GGATCCTGTG 
TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG GCCTAGCCTC TGTCCTCTCA 
GCCTGTGGCC TCCACATCCC CGCCGCCGAG GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510331.1
CDS	1402..5037
Translation	MAFQTPGTAVTHQKLGSQGVHLAVAGALQGHSCCCGCTVPLLPGNPGLAGVTSSTTWTGAEGQRMDLRTSWPRTRTTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPGPGRDQGGPHEEDPVWHQGAEPQRPRRRGLASVLSACGLHIPAAEA

Target ORF information:

RefSeq Version	XM_011512029.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512029.3

ORF Insert Sequence:

ATGGCATTTC AGACTCCAGG GACAGCTGTC ACCCACCAAA AACTGGGGTC ACAAGGGGTC 
CATCTGGCAG TGGCTGGAGC CTTGCAAGGG CACAGCTGCT GCTGCGGATG TACTGTTCCC 
CTCCTGCCTG GGAACCCTGG CCTTGCTGGG GTCACCTCCT CCACAACTTG GACTGGAGCA 
GAAGGACAAA GAATGGATCT GAGGACAAGC TGGCCCAGGA CCAGAACTAC CATCATCAAA 
GAGGGGATGC TGACCAAACA GAACAATTCA TTCCAGCGAT CAAAAAGGAG ATACTTTAAG 
CTTCGAGGGC GAACGCTTTA CTATGCCAAA ACGGCAAAGT CAATCATATT TGATGAGGTG 
GATCTGACAG ATGCCAGCGT AGCTGAATCC AGTACCAAAA ACGTCAACAA CAGTTTTACG 
GTCATAACTC CATGCAGGAA GCTCATCTTG TGTGCTGATA ACAGAAAAGA AATGGAAGAT 
TGGATTGCAG CATTAAAGAC TGTGCAGAAC AGGGAGCACT TTGAGCCCAC CCAGTACAGC 
ATGGACCACT TCTCAGGGAT GCACAATTGG TACGCCTGTT CCCACGCGAG GCCGACCTAC 
TGCAATGTGT GCCGTGAGGC TCTGTCTGGG GTCACGTCGC ACGGGCTGTC CTGCGAGGTG 
TGCAAATTTA AGGCCCACAA GCGCTGTGCT GTGCGTGCAA CCAATAACTG CAAGTGGACC 
ACACTGGCCT CGATCGGGAA GGACATCATT GAAGATGCAG ATGGGATTGC AATGCCCCAC 
CAGTGGTTGG AAGGAAACCT ACCTGTGAGC GCCAAGTGCA CTGTGTGCGA CAAGACCTGT 
GGCAGTGTGC TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCGTGTAAAG AATCCTTGCT GACCAAGTGC CCACTTGGCC TGTGCAAAGT GTCAGTCATC 
CCACCCACGG CTCTCAACAG CATCGACTCC GATGGGTTCT GGAAGGCCAG CTGTCCTCCT 
TCTTGCACAA GCCCACTGTT GGTCTTCGTC AATTCAAAAA GTGGGGACAA CCAGGGTGTG 
AAGTTCCTCA GAAGATTCAA ACAGCTACTA AACCCCGCCC AGGTCTTCGA CCTCATGAAC 
GGAGGCCCAC ACCTCGGCTT ACGGTTATTC CAGAAGTTTG ACACATTCCG GATTCTGGTT 
TGTGGCGGGG ATGGAAGTGT TGGCTGGGTC CTCTCCGAAA TCGACAGCCT CAACCTTCAT 
AAACAGTGTC AGCTGGGAGT GCTGCCGCTC GGCACAGGGA ACGACTTGGC CCGAGTACTG 
GGCTGGGGCT CAGCCTGCGA TGACGACACC CAGCTCCCCC AGATCTTGGA GAAGTTGGAG 
AGAGCCAGCA CCAAGATGCT GGACAGGTGG AGCGTCATGG CATACGAGGC CAAGCTCCCC 
CGGCAGGCCT CCTCCTCTAC CGTCACCGAA GACTTCAGCG AGGATTCCGA GGTACAGCAG 
ATTCTCTTCT ATGAAGACTC GGTTGCAGCC CACCTTTCTA AAATCCTCAC CTCGGACCAG 
CACTCGGTGG TCATCTCCTC GGCCAAAGTG CTCTGTGAGA CGGTGAAGGA CTTCGTGGCA 
CGGGTGGGGA AGGCCTATGA GAAGACGACC GAGAGCTCGG AGGAGTCAGA GGTCATGGCC 
AAGAAGTGCT CTGTCCTGAA AGAGAAGCTG GATTCCCTTC TCAAGACCTT GGACGATGAG 
TCCCAGGCCT CGTCCTCTCT GCCCAACCCG CCCCCCACCA TTGCCGAGGA GGCTGAAGAT 
GGAGATGGGT CGGGCAGCAT CTGCGGTTCC ACCGGAGACC GCTTGGTGGC ATCAGCTTGC 
CCGGCCCGGC CGCAGATATT CCGGCCTCGA GAACAGCTCA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGTCAGAT CATAGAACAC ACAGAAAAAG CTGTCGATGA GCAGAATGCC 
CAGACCCAGG AGCAGGAGGG CTTCGTCCTG GGCCTCTCTG AGTCAGAGGA GAAGATGGAC 
CACAGAGTGT GCCCACCACT GTCCCACAGC GAGAGCTTCG GGGTCCCCAA GGGGAGGAGC 
CAGCGCAAAG TGTCGAAATC TCCGTGTGAA AAGCTGATCA GCAAAGGGAG TCTGTCCCTA 
GGCAGTTCTG CTTCCCTTCC GCCCCAGCCG GGAAGCCGGG ACGGCCTGCC TGCGCTCAAC 
ACCAAGATCC TGTACCCAAA TGTCCGGGCT GGAATGTCTG GTTCCTTACC CGGTGGCTCA 
GTCATCAGTC GCCTGTTAAT TAATGCTGAT CCCTTCAACT CTGAACCAGA AACCCTAGAG 
TATTACACGG AGAAATGTGT CATGAACAAC TATTTTGGCA TTGGCCTGGA TGCGAAGATA 
TCCCTGGACT TTAACAACAA GCGCGATGAG CACCCAGAGA AGTGCAGGAG CCGAACCAAG 
AACATGATGT GGTATGGAGT TCTTGGAACC AAAGAGTTGC TGCACAGAAC CTACAAGAAC 
CTGGAGCAAA AGGTCTTGCT GGAGTGTGAC GGGCGACCCA TCCCACTCCC CAGTCTTCAG 
GGAATTGCTG TCCTTAACAT TCCCAGCTAT GCCGGAGGAA CCAACTTCTG GGGGGGTACC 
AAGGAAGATG ATACTTTCGC AGCTCCATCA TTCGATGACA AGATTCTGGA GGTGGTCGCC 
GTGTTCGGCA GCATGCAGAT GGCCGTCTCT CGAGTCATCA GGCTACAGCA TCATCGGATC 
GCCCAGTGTC GCACGGTGAA GATCTCCATC CTTGGGGATG AGGGCGTGCC TGTGCAGGTG 
GACGGAGAGG CCTGGGTCCA GCCGCCAGGG TACATTCGGA TTGTCCACAA GAACCGGGCA 
CAGACACTGA CCAGAGACAG GGCATTTGAG AGCACCCTGA AGTCCTGGGA AGACAAGCAG 
AAGTGCGAGC TGCCCCGCCC TCCATCCTGT TCCCTGCACC CGGAGATGCT GTCCGAGGAG 
GAGGCCACCC AGATGGACCA GTTTGGGCAG GCAGCAGGGG TCCTCATTCA CAGTATCCGA 
GAAATAGCTC AGTCTCACCG GGACATGGAG CAGGAACTGG CCCACGCCGT CAATGCCAGC 
TCCAAGTCCA TGGACCGTGT GTATGGCAAG CCCAGAACCA CAGAGGGGCT CAACTGCAGC 
TTCGTCCTGG AAATGGTGAA TAACTTCAGA GCTCTGCGCA GTGAGACGGA GCTGCTGCTG 
TCTGGGAAGA TGGCCCTGCA GCTGGATCCG CCTCAGAAGG AGCAGCTGGG GAGTGCTCTT 
GCCGAGATGG ACCGACAGCT CAGGAGGCTG GCAGACACCC CGTGGCTCTG CCAGTCCGCA 
GAGCCCGGCG ACGAAGAGAG TGTGATGCTG GATCTTGCCA AGCGCAGTCG CAGTGGTAAA 
TTCCGCCTCG TGACCAAGTT TAAAAAGGAG AAAAACAACA AGAACAAAGA AGCTCACAGT 
AGCCTGGGAG CCCCGGGACC TGGGCGTGAC CAAGGTGGGC CACATGAAGA GGATCCTGTG 
TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG GCCTAGCCTC TGTCCTCTCA 
GCCTGTGGCC TCCACATCCC CGCCGCCGAG GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512029.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510331.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu61924
Clone ID Related Accession (Same CDS sequence)	XM_011512029.3 , XM_011512029.2
Accession Version	XM_011512029.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTTC AGACTCCAGG GACAGCTGTC ACCCACCAAA AACTGGGGTC ACAAGGGGTC 
CATCTGGCAG TGGCTGGAGC CTTGCAAGGG CACAGCTGCT GCTGCGGATG TACTGTTCCC 
CTCCTGCCTG GGAACCCTGG CCTTGCTGGG GTCACCTCCT CCACAACTTG GACTGGAGCA 
GAAGGACAAA GAATGGATCT GAGGACAAGC TGGCCCAGGA CCAGAACTAC CATCATCAAA 
GAGGGGATGC TGACCAAACA GAACAATTCA TTCCAGCGAT CAAAAAGGAG ATACTTTAAG 
CTTCGAGGGC GAACGCTTTA CTATGCCAAA ACGGCAAAGT CAATCATATT TGATGAGGTG 
GATCTGACAG ATGCCAGCGT AGCTGAATCC AGTACCAAAA ACGTCAACAA CAGTTTTACG 
GTCATAACTC CATGCAGGAA GCTCATCTTG TGTGCTGATA ACAGAAAAGA AATGGAAGAT 
TGGATTGCAG CATTAAAGAC TGTGCAGAAC AGGGAGCACT TTGAGCCCAC CCAGTACAGC 
ATGGACCACT TCTCAGGGAT GCACAATTGG TACGCCTGTT CCCACGCGAG GCCGACCTAC 
TGCAATGTGT GCCGTGAGGC TCTGTCTGGG GTCACGTCGC ACGGGCTGTC CTGCGAGGTG 
TGCAAATTTA AGGCCCACAA GCGCTGTGCT GTGCGTGCAA CCAATAACTG CAAGTGGACC 
ACACTGGCCT CGATCGGGAA GGACATCATT GAAGATGCAG ATGGGATTGC AATGCCCCAC 
CAGTGGTTGG AAGGAAACCT ACCTGTGAGC GCCAAGTGCA CTGTGTGCGA CAAGACCTGT 
GGCAGTGTGC TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCGTGTAAAG AATCCTTGCT GACCAAGTGC CCACTTGGCC TGTGCAAAGT GTCAGTCATC 
CCACCCACGG CTCTCAACAG CATCGACTCC GATGGGTTCT GGAAGGCCAG CTGTCCTCCT 
TCTTGCACAA GCCCACTGTT GGTCTTCGTC AATTCAAAAA GTGGGGACAA CCAGGGTGTG 
AAGTTCCTCA GAAGATTCAA ACAGCTACTA AACCCCGCCC AGGTCTTCGA CCTCATGAAC 
GGAGGCCCAC ACCTCGGCTT ACGGTTATTC CAGAAGTTTG ACACATTCCG GATTCTGGTT 
TGTGGCGGGG ATGGAAGTGT TGGCTGGGTC CTCTCCGAAA TCGACAGCCT CAACCTTCAT 
AAACAGTGTC AGCTGGGAGT GCTGCCGCTC GGCACAGGGA ACGACTTGGC CCGAGTACTG 
GGCTGGGGCT CAGCCTGCGA TGACGACACC CAGCTCCCCC AGATCTTGGA GAAGTTGGAG 
AGAGCCAGCA CCAAGATGCT GGACAGGTGG AGCGTCATGG CATACGAGGC CAAGCTCCCC 
CGGCAGGCCT CCTCCTCTAC CGTCACCGAA GACTTCAGCG AGGATTCCGA GGTACAGCAG 
ATTCTCTTCT ATGAAGACTC GGTTGCAGCC CACCTTTCTA AAATCCTCAC CTCGGACCAG 
CACTCGGTGG TCATCTCCTC GGCCAAAGTG CTCTGTGAGA CGGTGAAGGA CTTCGTGGCA 
CGGGTGGGGA AGGCCTATGA GAAGACGACC GAGAGCTCGG AGGAGTCAGA GGTCATGGCC 
AAGAAGTGCT CTGTCCTGAA AGAGAAGCTG GATTCCCTTC TCAAGACCTT GGACGATGAG 
TCCCAGGCCT CGTCCTCTCT GCCCAACCCG CCCCCCACCA TTGCCGAGGA GGCTGAAGAT 
GGAGATGGGT CGGGCAGCAT CTGCGGTTCC ACCGGAGACC GCTTGGTGGC ATCAGCTTGC 
CCGGCCCGGC CGCAGATATT CCGGCCTCGA GAACAGCTCA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGTCAGAT CATAGAACAC ACAGAAAAAG CTGTCGATGA GCAGAATGCC 
CAGACCCAGG AGCAGGAGGG CTTCGTCCTG GGCCTCTCTG AGTCAGAGGA GAAGATGGAC 
CACAGAGTGT GCCCACCACT GTCCCACAGC GAGAGCTTCG GGGTCCCCAA GGGGAGGAGC 
CAGCGCAAAG TGTCGAAATC TCCGTGTGAA AAGCTGATCA GCAAAGGGAG TCTGTCCCTA 
GGCAGTTCTG CTTCCCTTCC GCCCCAGCCG GGAAGCCGGG ACGGCCTGCC TGCGCTCAAC 
ACCAAGATCC TGTACCCAAA TGTCCGGGCT GGAATGTCTG GTTCCTTACC CGGTGGCTCA 
GTCATCAGTC GCCTGTTAAT TAATGCTGAT CCCTTCAACT CTGAACCAGA AACCCTAGAG 
TATTACACGG AGAAATGTGT CATGAACAAC TATTTTGGCA TTGGCCTGGA TGCGAAGATA 
TCCCTGGACT TTAACAACAA GCGCGATGAG CACCCAGAGA AGTGCAGGAG CCGAACCAAG 
AACATGATGT GGTATGGAGT TCTTGGAACC AAAGAGTTGC TGCACAGAAC CTACAAGAAC 
CTGGAGCAAA AGGTCTTGCT GGAGTGTGAC GGGCGACCCA TCCCACTCCC CAGTCTTCAG 
GGAATTGCTG TCCTTAACAT TCCCAGCTAT GCCGGAGGAA CCAACTTCTG GGGGGGTACC 
AAGGAAGATG ATACTTTCGC AGCTCCATCA TTCGATGACA AGATTCTGGA GGTGGTCGCC 
GTGTTCGGCA GCATGCAGAT GGCCGTCTCT CGAGTCATCA GGCTACAGCA TCATCGGATC 
GCCCAGTGTC GCACGGTGAA GATCTCCATC CTTGGGGATG AGGGCGTGCC TGTGCAGGTG 
GACGGAGAGG CCTGGGTCCA GCCGCCAGGG TACATTCGGA TTGTCCACAA GAACCGGGCA 
CAGACACTGA CCAGAGACAG GGCATTTGAG AGCACCCTGA AGTCCTGGGA AGACAAGCAG 
AAGTGCGAGC TGCCCCGCCC TCCATCCTGT TCCCTGCACC CGGAGATGCT GTCCGAGGAG 
GAGGCCACCC AGATGGACCA GTTTGGGCAG GCAGCAGGGG TCCTCATTCA CAGTATCCGA 
GAAATAGCTC AGTCTCACCG GGACATGGAG CAGGAACTGG CCCACGCCGT CAATGCCAGC 
TCCAAGTCCA TGGACCGTGT GTATGGCAAG CCCAGAACCA CAGAGGGGCT CAACTGCAGC 
TTCGTCCTGG AAATGGTGAA TAACTTCAGA GCTCTGCGCA GTGAGACGGA GCTGCTGCTG 
TCTGGGAAGA TGGCCCTGCA GCTGGATCCG CCTCAGAAGG AGCAGCTGGG GAGTGCTCTT 
GCCGAGATGG ACCGACAGCT CAGGAGGCTG GCAGACACCC CGTGGCTCTG CCAGTCCGCA 
GAGCCCGGCG ACGAAGAGAG TGTGATGCTG GATCTTGCCA AGCGCAGTCG CAGTGGTAAA 
TTCCGCCTCG TGACCAAGTT TAAAAAGGAG AAAAACAACA AGAACAAAGA AGCTCACAGT 
AGCCTGGGAG CCCCGGGACC TGGGCGTGAC CAAGGTGGGC CACATGAAGA GGATCCTGTG 
TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG GCCTAGCCTC TGTCCTCTCA 
GCCTGTGGCC TCCACATCCC CGCCGCCGAG GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510331.1
CDS	1401..5036
Translation	MAFQTPGTAVTHQKLGSQGVHLAVAGALQGHSCCCGCTVPLLPGNPGLAGVTSSTTWTGAEGQRMDLRTSWPRTRTTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPGPGRDQGGPHEEDPVWHQGAEPQRPRRRGLASVLSACGLHIPAAEA

Target ORF information:

RefSeq Version	XM_011512029.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512029.2

ORF Insert Sequence:

ATGGCATTTC AGACTCCAGG GACAGCTGTC ACCCACCAAA AACTGGGGTC ACAAGGGGTC 
CATCTGGCAG TGGCTGGAGC CTTGCAAGGG CACAGCTGCT GCTGCGGATG TACTGTTCCC 
CTCCTGCCTG GGAACCCTGG CCTTGCTGGG GTCACCTCCT CCACAACTTG GACTGGAGCA 
GAAGGACAAA GAATGGATCT GAGGACAAGC TGGCCCAGGA CCAGAACTAC CATCATCAAA 
GAGGGGATGC TGACCAAACA GAACAATTCA TTCCAGCGAT CAAAAAGGAG ATACTTTAAG 
CTTCGAGGGC GAACGCTTTA CTATGCCAAA ACGGCAAAGT CAATCATATT TGATGAGGTG 
GATCTGACAG ATGCCAGCGT AGCTGAATCC AGTACCAAAA ACGTCAACAA CAGTTTTACG 
GTCATAACTC CATGCAGGAA GCTCATCTTG TGTGCTGATA ACAGAAAAGA AATGGAAGAT 
TGGATTGCAG CATTAAAGAC TGTGCAGAAC AGGGAGCACT TTGAGCCCAC CCAGTACAGC 
ATGGACCACT TCTCAGGGAT GCACAATTGG TACGCCTGTT CCCACGCGAG GCCGACCTAC 
TGCAATGTGT GCCGTGAGGC TCTGTCTGGG GTCACGTCGC ACGGGCTGTC CTGCGAGGTG 
TGCAAATTTA AGGCCCACAA GCGCTGTGCT GTGCGTGCAA CCAATAACTG CAAGTGGACC 
ACACTGGCCT CGATCGGGAA GGACATCATT GAAGATGCAG ATGGGATTGC AATGCCCCAC 
CAGTGGTTGG AAGGAAACCT ACCTGTGAGC GCCAAGTGCA CTGTGTGCGA CAAGACCTGT 
GGCAGTGTGC TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCGTGTAAAG AATCCTTGCT GACCAAGTGC CCACTTGGCC TGTGCAAAGT GTCAGTCATC 
CCACCCACGG CTCTCAACAG CATCGACTCC GATGGGTTCT GGAAGGCCAG CTGTCCTCCT 
TCTTGCACAA GCCCACTGTT GGTCTTCGTC AATTCAAAAA GTGGGGACAA CCAGGGTGTG 
AAGTTCCTCA GAAGATTCAA ACAGCTACTA AACCCCGCCC AGGTCTTCGA CCTCATGAAC 
GGAGGCCCAC ACCTCGGCTT ACGGTTATTC CAGAAGTTTG ACACATTCCG GATTCTGGTT 
TGTGGCGGGG ATGGAAGTGT TGGCTGGGTC CTCTCCGAAA TCGACAGCCT CAACCTTCAT 
AAACAGTGTC AGCTGGGAGT GCTGCCGCTC GGCACAGGGA ACGACTTGGC CCGAGTACTG 
GGCTGGGGCT CAGCCTGCGA TGACGACACC CAGCTCCCCC AGATCTTGGA GAAGTTGGAG 
AGAGCCAGCA CCAAGATGCT GGACAGGTGG AGCGTCATGG CATACGAGGC CAAGCTCCCC 
CGGCAGGCCT CCTCCTCTAC CGTCACCGAA GACTTCAGCG AGGATTCCGA GGTACAGCAG 
ATTCTCTTCT ATGAAGACTC GGTTGCAGCC CACCTTTCTA AAATCCTCAC CTCGGACCAG 
CACTCGGTGG TCATCTCCTC GGCCAAAGTG CTCTGTGAGA CGGTGAAGGA CTTCGTGGCA 
CGGGTGGGGA AGGCCTATGA GAAGACGACC GAGAGCTCGG AGGAGTCAGA GGTCATGGCC 
AAGAAGTGCT CTGTCCTGAA AGAGAAGCTG GATTCCCTTC TCAAGACCTT GGACGATGAG 
TCCCAGGCCT CGTCCTCTCT GCCCAACCCG CCCCCCACCA TTGCCGAGGA GGCTGAAGAT 
GGAGATGGGT CGGGCAGCAT CTGCGGTTCC ACCGGAGACC GCTTGGTGGC ATCAGCTTGC 
CCGGCCCGGC CGCAGATATT CCGGCCTCGA GAACAGCTCA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGTCAGAT CATAGAACAC ACAGAAAAAG CTGTCGATGA GCAGAATGCC 
CAGACCCAGG AGCAGGAGGG CTTCGTCCTG GGCCTCTCTG AGTCAGAGGA GAAGATGGAC 
CACAGAGTGT GCCCACCACT GTCCCACAGC GAGAGCTTCG GGGTCCCCAA GGGGAGGAGC 
CAGCGCAAAG TGTCGAAATC TCCGTGTGAA AAGCTGATCA GCAAAGGGAG TCTGTCCCTA 
GGCAGTTCTG CTTCCCTTCC GCCCCAGCCG GGAAGCCGGG ACGGCCTGCC TGCGCTCAAC 
ACCAAGATCC TGTACCCAAA TGTCCGGGCT GGAATGTCTG GTTCCTTACC CGGTGGCTCA 
GTCATCAGTC GCCTGTTAAT TAATGCTGAT CCCTTCAACT CTGAACCAGA AACCCTAGAG 
TATTACACGG AGAAATGTGT CATGAACAAC TATTTTGGCA TTGGCCTGGA TGCGAAGATA 
TCCCTGGACT TTAACAACAA GCGCGATGAG CACCCAGAGA AGTGCAGGAG CCGAACCAAG 
AACATGATGT GGTATGGAGT TCTTGGAACC AAAGAGTTGC TGCACAGAAC CTACAAGAAC 
CTGGAGCAAA AGGTCTTGCT GGAGTGTGAC GGGCGACCCA TCCCACTCCC CAGTCTTCAG 
GGAATTGCTG TCCTTAACAT TCCCAGCTAT GCCGGAGGAA CCAACTTCTG GGGGGGTACC 
AAGGAAGATG ATACTTTCGC AGCTCCATCA TTCGATGACA AGATTCTGGA GGTGGTCGCC 
GTGTTCGGCA GCATGCAGAT GGCCGTCTCT CGAGTCATCA GGCTACAGCA TCATCGGATC 
GCCCAGTGTC GCACGGTGAA GATCTCCATC CTTGGGGATG AGGGCGTGCC TGTGCAGGTG 
GACGGAGAGG CCTGGGTCCA GCCGCCAGGG TACATTCGGA TTGTCCACAA GAACCGGGCA 
CAGACACTGA CCAGAGACAG GGCATTTGAG AGCACCCTGA AGTCCTGGGA AGACAAGCAG 
AAGTGCGAGC TGCCCCGCCC TCCATCCTGT TCCCTGCACC CGGAGATGCT GTCCGAGGAG 
GAGGCCACCC AGATGGACCA GTTTGGGCAG GCAGCAGGGG TCCTCATTCA CAGTATCCGA 
GAAATAGCTC AGTCTCACCG GGACATGGAG CAGGAACTGG CCCACGCCGT CAATGCCAGC 
TCCAAGTCCA TGGACCGTGT GTATGGCAAG CCCAGAACCA CAGAGGGGCT CAACTGCAGC 
TTCGTCCTGG AAATGGTGAA TAACTTCAGA GCTCTGCGCA GTGAGACGGA GCTGCTGCTG 
TCTGGGAAGA TGGCCCTGCA GCTGGATCCG CCTCAGAAGG AGCAGCTGGG GAGTGCTCTT 
GCCGAGATGG ACCGACAGCT CAGGAGGCTG GCAGACACCC CGTGGCTCTG CCAGTCCGCA 
GAGCCCGGCG ACGAAGAGAG TGTGATGCTG GATCTTGCCA AGCGCAGTCG CAGTGGTAAA 
TTCCGCCTCG TGACCAAGTT TAAAAAGGAG AAAAACAACA AGAACAAAGA AGCTCACAGT 
AGCCTGGGAG CCCCGGGACC TGGGCGTGAC CAAGGTGGGC CACATGAAGA GGATCCTGTG 
TGGCATCAAG GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG GCCTAGCCTC TGTCCTCTCA 
GCCTGTGGCC TCCACATCCC CGCCGCCGAG GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512030.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510332.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X4, mRNA.

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CloneID	OHu61925
Clone ID Related Accession (Same CDS sequence)	XM_011512030.2
Accession Version	XM_011512030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTGA TTGCTTTCCC CACAAAGACC ATCATCAAAG AGGGGATGCT GACCAAACAG 
AACAATTCAT TCCAGCGATC AAAAAGGAGA TACTTTAAGC TTCGAGGGCG AACGCTTTAC 
TATGCCAAAA CGGCAAAGTC AATCATATTT GATGAGGTGG ATCTGACAGA TGCCAGCGTA 
GCTGAATCCA GTACCAAAAA CGTCAACAAC AGTTTTACGG TCATAACTCC ATGCAGGAAG 
CTCATCTTGT GTGCTGATAA CAGAAAAGAA ATGGAAGATT GGATTGCAGC ATTAAAGACT 
GTGCAGAACA GGGAGCACTT TGAGCCCACC CAGTACAGCA TGGACCACTT CTCAGGGATG 
CACAATTGGT ACGCCTGTTC CCACGCGAGG CCGACCTACT GCAATGTGTG CCGTGAGGCT 
CTGTCTGGGG TCACGTCGCA CGGGCTGTCC TGCGAGGTGT GCAAATTTAA GGCCCACAAG 
CGCTGTGCTG TGCGTGCAAC CAATAACTGC AAGTGGACCA CACTGGCCTC GATCGGGAAG 
GACATCATTG AAGATGCAGA TGGGATTGCA ATGCCCCACC AGTGGTTGGA AGGAAACCTA 
CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC AAGACCTGTG GCAGTGTGCT GCGCCTGCAG 
GACTGGCGCT GCCTCTGGTG CAAGGCCATG GTTCACACAT CGTGTAAAGA ATCCTTGCTG 
ACCAAGTGCC CACTTGGCCT GTGCAAAGTG TCAGTCATCC CACCCACGGC TCTCAACAGC 
ATCGACTCCG ATGGGTTCTG GAAGGCCAGC TGTCCTCCTT CTTGCACAAG CCCACTGTTG 
GTCTTCGTCA ATTCAAAAAG TGGGGACAAC CAGGGTGTGA AGTTCCTCAG AAGATTCAAA 
CAGCTACTAA ACCCCGCCCA GGTCTTCGAC CTCATGAACG GAGGCCCACA CCTCGGCTTA 
CGGTTATTCC AGAAGTTTGA CACATTCCGG ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT 
GGCTGGGTCC TCTCCGAAAT CGACAGCCTC AACCTTCATA AACAGTGTCA GCTGGGAGTG 
CTGCCGCTCG GCACAGGGAA CGACTTGGCC CGAGTACTGG GCTGGGGCTC AGCCTGCGAT 
GACGACACCC AGCTCCCCCA GATCTTGGAG AAGTTGGAGA GAGCCAGCAC CAAGATGCTG 
GACAGGTGGA GCGTCATGGC ATACGAGGCC AAGCTCCCCC GGCAGGCCTC CTCCTCTACC 
GTCACCGAAG ACTTCAGCGA GGATTCCGAG GTACAGCAGA TTCTCTTCTA TGAAGACTCG 
GTTGCAGCCC ACCTTTCTAA AATCCTCACC TCGGACCAGC ACTCGGTGGT CATCTCCTCG 
GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC TTCGTGGCAC GGGTGGGGAA GGCCTATGAG 
AAGACGACCG AGAGCTCGGA GGAGTCAGAG GTCATGGCCA AGAAGTGCTC TGTCCTGAAA 
GAGAAGCTGG ATTCCCTTCT CAAGACCTTG GACGATGAGT CCCAGGCCTC GTCCTCTCTG 
CCCAACCCGC CCCCCACCAT TGCCGAGGAG GCTGAAGATG GAGATGGGTC GGGCAGCATC 
TGCGGTTCCA CCGGAGACCG CTTGGTGGCA TCAGCTTGCC CGGCCCGGCC GCAGATATTC 
CGGCCTCGAG AACAGCTCAT GCTGAGAGCC AACAGCCTGA AGAAAGCAAT TCGTCAGATC 
ATAGAACACA CAGAAAAAGC TGTCGATGAG CAGAATGCCC AGACCCAGGA GCAGGAGGGC 
TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG AAGATGGACC ACAGAGTGTG CCCACCACTG 
TCCCACAGCG AGAGCTTCGG GGTCCCCAAG GGGAGGAGCC AGCGCAAAGT GTCGAAATCT 
CCGTGTGAAA AGCTGATCAG CAAAGGGAGT CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG 
CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT GCGCTCAACA CCAAGATCCT GTACCCAAAT 
GTCCGGGCTG GAATGTCTGG TTCCTTACCC GGTGGCTCAG TCATCAGTCG CCTGTTAATT 
AATGCTGATC CCTTCAACTC TGAACCAGAA ACCCTAGAGT ATTACACGGA GAAATGTGTC 
ATGAACAACT ATTTTGGCAT TGGCCTGGAT GCGAAGATAT CCCTGGACTT TAACAACAAG 
CGCGATGAGC ACCCAGAGAA GTGCAGGAGC CGAACCAAGA ACATGATGTG GTATGGAGTT 
CTTGGAACCA AAGAGTTGCT GCACAGAACC TACAAGAACC TGGAGCAAAA GGTCTTGCTG 
GAGTGTGACG GGCGACCCAT CCCACTCCCC AGTCTTCAGG GAATTGCTGT CCTTAACATT 
CCCAGCTATG CCGGAGGAAC CAACTTCTGG GGGGGTACCA AGGAAGATGA TACTTTCGCA 
GCTCCATCAT TCGATGACAA GATTCTGGAG GTGGTCGCCG TGTTCGGCAG CATGCAGATG 
GCCGTCTCTC GAGTCATCAG GCTACAGCAT CATCGGATCG CCCAGTGTCG CACGGTGAAG 
ATCTCCATCC TTGGGGATGA GGGCGTGCCT GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG 
CCGCCAGGGT ACATTCGGAT TGTCCACAAG AACCGGGCAC AGACACTGAC CAGAGACAGG 
GCATTTGAGA GCACCCTGAA GTCCTGGGAA GACAAGCAGA AGTGCGAGCT GCCCCGCCCT 
CCATCCTGTT CCCTGCACCC GGAGATGCTG TCCGAGGAGG AGGCCACCCA GATGGACCAG 
TTTGGGCAGG CAGCAGGGGT CCTCATTCAC AGTATCCGAG AAATAGCTCA GTCTCACCGG 
GACATGGAGC AGGAACTGGC CCACGCCGTC AATGCCAGCT CCAAGTCCAT GGACCGTGTG 
TATGGCAAGC CCAGAACCAC AGAGGGGCTC AACTGCAGCT TCGTCCTGGA AATGGTGAAT 
AACTTCAGAG CTCTGCGCAG TGAGACGGAG CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG 
CTGGATCCGC CTCAGAAGGA GCAGCTGGGG AGTGCTCTTG CCGAGATGGA CCGACAGCTC 
AGGAGGCTGG CAGACACCCC GTGGCTCTGC CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT 
GTGATGCTGG ATCTTGCCAA GCGCAGTCGC AGTGGTAAAT TCCGCCTCGT GACCAAGTTT 
AAAAAGGAGA AAAACAACAA GAACAAAGAA GCTCACAGTA GCCTGGGAGC CCCGGTTCAC 
CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG CTGGAGCACC TCAGTCTCTG TGAGTATAAG 
GACATCTTCA CACGGCACGA CATCCGGGGC TCTGAGCTCC TGCACCTGGA GCGGAGGGAC 
CTCAAGGACC TGGGCGTGAC CAAGGTGGGC CACATGAAGA GGATCCTGTG TGGCATCAAG 
GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510332.1
CDS	198..3713
Translation	MEVIAFPTKTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_011512030.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512030.2

ORF Insert Sequence:

ATGGAAGTGA TTGCTTTCCC CACAAAGACC ATCATCAAAG AGGGGATGCT GACCAAACAG 
AACAATTCAT TCCAGCGATC AAAAAGGAGA TACTTTAAGC TTCGAGGGCG AACGCTTTAC 
TATGCCAAAA CGGCAAAGTC AATCATATTT GATGAGGTGG ATCTGACAGA TGCCAGCGTA 
GCTGAATCCA GTACCAAAAA CGTCAACAAC AGTTTTACGG TCATAACTCC ATGCAGGAAG 
CTCATCTTGT GTGCTGATAA CAGAAAAGAA ATGGAAGATT GGATTGCAGC ATTAAAGACT 
GTGCAGAACA GGGAGCACTT TGAGCCCACC CAGTACAGCA TGGACCACTT CTCAGGGATG 
CACAATTGGT ACGCCTGTTC CCACGCGAGG CCGACCTACT GCAATGTGTG CCGTGAGGCT 
CTGTCTGGGG TCACGTCGCA CGGGCTGTCC TGCGAGGTGT GCAAATTTAA GGCCCACAAG 
CGCTGTGCTG TGCGTGCAAC CAATAACTGC AAGTGGACCA CACTGGCCTC GATCGGGAAG 
GACATCATTG AAGATGCAGA TGGGATTGCA ATGCCCCACC AGTGGTTGGA AGGAAACCTA 
CCTGTGAGCG CCAAGTGCAC TGTGTGCGAC AAGACCTGTG GCAGTGTGCT GCGCCTGCAG 
GACTGGCGCT GCCTCTGGTG CAAGGCCATG GTTCACACAT CGTGTAAAGA ATCCTTGCTG 
ACCAAGTGCC CACTTGGCCT GTGCAAAGTG TCAGTCATCC CACCCACGGC TCTCAACAGC 
ATCGACTCCG ATGGGTTCTG GAAGGCCAGC TGTCCTCCTT CTTGCACAAG CCCACTGTTG 
GTCTTCGTCA ATTCAAAAAG TGGGGACAAC CAGGGTGTGA AGTTCCTCAG AAGATTCAAA 
CAGCTACTAA ACCCCGCCCA GGTCTTCGAC CTCATGAACG GAGGCCCACA CCTCGGCTTA 
CGGTTATTCC AGAAGTTTGA CACATTCCGG ATTCTGGTTT GTGGCGGGGA TGGAAGTGTT 
GGCTGGGTCC TCTCCGAAAT CGACAGCCTC AACCTTCATA AACAGTGTCA GCTGGGAGTG 
CTGCCGCTCG GCACAGGGAA CGACTTGGCC CGAGTACTGG GCTGGGGCTC AGCCTGCGAT 
GACGACACCC AGCTCCCCCA GATCTTGGAG AAGTTGGAGA GAGCCAGCAC CAAGATGCTG 
GACAGGTGGA GCGTCATGGC ATACGAGGCC AAGCTCCCCC GGCAGGCCTC CTCCTCTACC 
GTCACCGAAG ACTTCAGCGA GGATTCCGAG GTACAGCAGA TTCTCTTCTA TGAAGACTCG 
GTTGCAGCCC ACCTTTCTAA AATCCTCACC TCGGACCAGC ACTCGGTGGT CATCTCCTCG 
GCCAAAGTGC TCTGTGAGAC GGTGAAGGAC TTCGTGGCAC GGGTGGGGAA GGCCTATGAG 
AAGACGACCG AGAGCTCGGA GGAGTCAGAG GTCATGGCCA AGAAGTGCTC TGTCCTGAAA 
GAGAAGCTGG ATTCCCTTCT CAAGACCTTG GACGATGAGT CCCAGGCCTC GTCCTCTCTG 
CCCAACCCGC CCCCCACCAT TGCCGAGGAG GCTGAAGATG GAGATGGGTC GGGCAGCATC 
TGCGGTTCCA CCGGAGACCG CTTGGTGGCA TCAGCTTGCC CGGCCCGGCC GCAGATATTC 
CGGCCTCGAG AACAGCTCAT GCTGAGAGCC AACAGCCTGA AGAAAGCAAT TCGTCAGATC 
ATAGAACACA CAGAAAAAGC TGTCGATGAG CAGAATGCCC AGACCCAGGA GCAGGAGGGC 
TTCGTCCTGG GCCTCTCTGA GTCAGAGGAG AAGATGGACC ACAGAGTGTG CCCACCACTG 
TCCCACAGCG AGAGCTTCGG GGTCCCCAAG GGGAGGAGCC AGCGCAAAGT GTCGAAATCT 
CCGTGTGAAA AGCTGATCAG CAAAGGGAGT CTGTCCCTAG GCAGTTCTGC TTCCCTTCCG 
CCCCAGCCGG GAAGCCGGGA CGGCCTGCCT GCGCTCAACA CCAAGATCCT GTACCCAAAT 
GTCCGGGCTG GAATGTCTGG TTCCTTACCC GGTGGCTCAG TCATCAGTCG CCTGTTAATT 
AATGCTGATC CCTTCAACTC TGAACCAGAA ACCCTAGAGT ATTACACGGA GAAATGTGTC 
ATGAACAACT ATTTTGGCAT TGGCCTGGAT GCGAAGATAT CCCTGGACTT TAACAACAAG 
CGCGATGAGC ACCCAGAGAA GTGCAGGAGC CGAACCAAGA ACATGATGTG GTATGGAGTT 
CTTGGAACCA AAGAGTTGCT GCACAGAACC TACAAGAACC TGGAGCAAAA GGTCTTGCTG 
GAGTGTGACG GGCGACCCAT CCCACTCCCC AGTCTTCAGG GAATTGCTGT CCTTAACATT 
CCCAGCTATG CCGGAGGAAC CAACTTCTGG GGGGGTACCA AGGAAGATGA TACTTTCGCA 
GCTCCATCAT TCGATGACAA GATTCTGGAG GTGGTCGCCG TGTTCGGCAG CATGCAGATG 
GCCGTCTCTC GAGTCATCAG GCTACAGCAT CATCGGATCG CCCAGTGTCG CACGGTGAAG 
ATCTCCATCC TTGGGGATGA GGGCGTGCCT GTGCAGGTGG ACGGAGAGGC CTGGGTCCAG 
CCGCCAGGGT ACATTCGGAT TGTCCACAAG AACCGGGCAC AGACACTGAC CAGAGACAGG 
GCATTTGAGA GCACCCTGAA GTCCTGGGAA GACAAGCAGA AGTGCGAGCT GCCCCGCCCT 
CCATCCTGTT CCCTGCACCC GGAGATGCTG TCCGAGGAGG AGGCCACCCA GATGGACCAG 
TTTGGGCAGG CAGCAGGGGT CCTCATTCAC AGTATCCGAG AAATAGCTCA GTCTCACCGG 
GACATGGAGC AGGAACTGGC CCACGCCGTC AATGCCAGCT CCAAGTCCAT GGACCGTGTG 
TATGGCAAGC CCAGAACCAC AGAGGGGCTC AACTGCAGCT TCGTCCTGGA AATGGTGAAT 
AACTTCAGAG CTCTGCGCAG TGAGACGGAG CTGCTGCTGT CTGGGAAGAT GGCCCTGCAG 
CTGGATCCGC CTCAGAAGGA GCAGCTGGGG AGTGCTCTTG CCGAGATGGA CCGACAGCTC 
AGGAGGCTGG CAGACACCCC GTGGCTCTGC CAGTCCGCAG AGCCCGGCGA CGAAGAGAGT 
GTGATGCTGG ATCTTGCCAA GCGCAGTCGC AGTGGTAAAT TCCGCCTCGT GACCAAGTTT 
AAAAAGGAGA AAAACAACAA GAACAAAGAA GCTCACAGTA GCCTGGGAGC CCCGGTTCAC 
CTCTGGGGGA CAGAGGAGGT TGCTGCCTGG CTGGAGCACC TCAGTCTCTG TGAGTATAAG 
GACATCTTCA CACGGCACGA CATCCGGGGC TCTGAGCTCC TGCACCTGGA GCGGAGGGAC 
CTCAAGGACC TGGGCGTGAC CAAGGTGGGC CACATGAAGA GGATCCTGTG TGGCATCAAG 
GAGCTGAGCC GCAGCGCCCC CGCCGTCGAG GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512032.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510334.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X6, mRNA.

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CloneID	OHu61926
Clone ID Related Accession (Same CDS sequence)	XM_011512032.3 , XM_011512032.2 , XM_011512031.2 , XM_011512031.3
Accession Version	XM_011512032.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512032.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACCA AACAGAACAA TTCATTCCAG CGATCAAAAA GGAGATACTT TAAGCTTCGA 
GGGCGAACGC TTTACTATGC CAAAACGGCA AAGTCAATCA TATTTGATGA GGTGGATCTG 
ACAGATGCCA GCGTAGCTGA ATCCAGTACC AAAAACGTCA ACAACAGTTT TACGGTCATA 
ACTCCATGCA GGAAGCTCAT CTTGTGTGCT GATAACAGAA AAGAAATGGA AGATTGGATT 
GCAGCATTAA AGACTGTGCA GAACAGGGAG CACTTTGAGC CCACCCAGTA CAGCATGGAC 
CACTTCTCAG GGATGCACAA TTGGTACGCC TGTTCCCACG CGAGGCCGAC CTACTGCAAT 
GTGTGCCGTG AGGCTCTGTC TGGGGTCACG TCGCACGGGC TGTCCTGCGA GGTGTGCAAA 
TTTAAGGCCC ACAAGCGCTG TGCTGTGCGT GCAACCAATA ACTGCAAGTG GACCACACTG 
GCCTCGATCG GGAAGGACAT CATTGAAGAT GCAGATGGGA TTGCAATGCC CCACCAGTGG 
TTGGAAGGAA ACCTACCTGT GAGCGCCAAG TGCACTGTGT GCGACAAGAC CTGTGGCAGT 
GTGCTGCGCC TGCAGGACTG GCGCTGCCTC TGGTGCAAGG CCATGGTTCA CACATCGTGT 
AAAGAATCCT TGCTGACCAA GTGCCCACTT GGCCTGTGCA AAGTGTCAGT CATCCCACCC 
ACGGCTCTCA ACAGCATCGA CTCCGATGGG TTCTGGAAGG CCAGCTGTCC TCCTTCTTGC 
ACAAGCCCAC TGTTGGTCTT CGTCAATTCA AAAAGTGGGG ACAACCAGGG TGTGAAGTTC 
CTCAGAAGAT TCAAACAGCT ACTAAACCCC GCCCAGGTCT TCGACCTCAT GAACGGAGGC 
CCACACCTCG GCTTACGGTT ATTCCAGAAG TTTGACACAT TCCGGATTCT GGTTTGTGGC 
GGGGATGGAA GTGTTGGCTG GGTCCTCTCC GAAATCGACA GCCTCAACCT TCATAAACAG 
TGTCAGCTGG GAGTGCTGCC GCTCGGCACA GGGAACGACT TGGCCCGAGT ACTGGGCTGG 
GGCTCAGCCT GCGATGACGA CACCCAGCTC CCCCAGATCT TGGAGAAGTT GGAGAGAGCC 
AGCACCAAGA TGCTGGACAG GTGGAGCGTC ATGGCATACG AGGCCAAGCT CCCCCGGCAG 
GCCTCCTCCT CTACCGTCAC CGAAGACTTC AGCGAGGATT CCGAGGTACA GCAGATTCTC 
TTCTATGAAG ACTCGGTTGC AGCCCACCTT TCTAAAATCC TCACCTCGGA CCAGCACTCG 
GTGGTCATCT CCTCGGCCAA AGTGCTCTGT GAGACGGTGA AGGACTTCGT GGCACGGGTG 
GGGAAGGCCT ATGAGAAGAC GACCGAGAGC TCGGAGGAGT CAGAGGTCAT GGCCAAGAAG 
TGCTCTGTCC TGAAAGAGAA GCTGGATTCC CTTCTCAAGA CCTTGGACGA TGAGTCCCAG 
GCCTCGTCCT CTCTGCCCAA CCCGCCCCCC ACCATTGCCG AGGAGGCTGA AGATGGAGAT 
GGGTCGGGCA GCATCTGCGG TTCCACCGGA GACCGCTTGG TGGCATCAGC TTGCCCGGCC 
CGGCCGCAGA TATTCCGGCC TCGAGAACAG CTCATGCTGA GAGCCAACAG CCTGAAGAAA 
GCAATTCGTC AGATCATAGA ACACACAGAA AAAGCTGTCG ATGAGCAGAA TGCCCAGACC 
CAGGAGCAGG AGGGCTTCGT CCTGGGCCTC TCTGAGTCAG AGGAGAAGAT GGACCACAGA 
GTGTGCCCAC CACTGTCCCA CAGCGAGAGC TTCGGGGTCC CCAAGGGGAG GAGCCAGCGC 
AAAGTGTCGA AATCTCCGTG TGAAAAGCTG ATCAGCAAAG GGAGTCTGTC CCTAGGCAGT 
TCTGCTTCCC TTCCGCCCCA GCCGGGAAGC CGGGACGGCC TGCCTGCGCT CAACACCAAG 
ATCCTGTACC CAAATGTCCG GGCTGGAATG TCTGGTTCCT TACCCGGTGG CTCAGTCATC 
AGTCGCCTGT TAATTAATGC TGATCCCTTC AACTCTGAAC CAGAAACCCT AGAGTATTAC 
ACGGAGAAAT GTGTCATGAA CAACTATTTT GGCATTGGCC TGGATGCGAA GATATCCCTG 
GACTTTAACA ACAAGCGCGA TGAGCACCCA GAGAAGTGCA GGAGCCGAAC CAAGAACATG 
ATGTGGTATG GAGTTCTTGG AACCAAAGAG TTGCTGCACA GAACCTACAA GAACCTGGAG 
CAAAAGGTCT TGCTGGAGTG TGACGGGCGA CCCATCCCAC TCCCCAGTCT TCAGGGAATT 
GCTGTCCTTA ACATTCCCAG CTATGCCGGA GGAACCAACT TCTGGGGGGG TACCAAGGAA 
GATGATACTT TCGCAGCTCC ATCATTCGAT GACAAGATTC TGGAGGTGGT CGCCGTGTTC 
GGCAGCATGC AGATGGCCGT CTCTCGAGTC ATCAGGCTAC AGCATCATCG GATCGCCCAG 
TGTCGCACGG TGAAGATCTC CATCCTTGGG GATGAGGGCG TGCCTGTGCA GGTGGACGGA 
GAGGCCTGGG TCCAGCCGCC AGGGTACATT CGGATTGTCC ACAAGAACCG GGCACAGACA 
CTGACCAGAG ACAGGGCATT TGAGAGCACC CTGAAGTCCT GGGAAGACAA GCAGAAGTGC 
GAGCTGCCCC GCCCTCCATC CTGTTCCCTG CACCCGGAGA TGCTGTCCGA GGAGGAGGCC 
ACCCAGATGG ACCAGTTTGG GCAGGCAGCA GGGGTCCTCA TTCACAGTAT CCGAGAAATA 
GCTCAGTCTC ACCGGGACAT GGAGCAGGAA CTGGCCCACG CCGTCAATGC CAGCTCCAAG 
TCCATGGACC GTGTGTATGG CAAGCCCAGA ACCACAGAGG GGCTCAACTG CAGCTTCGTC 
CTGGAAATGG TGAATAACTT CAGAGCTCTG CGCAGTGAGA CGGAGCTGCT GCTGTCTGGG 
AAGATGGCCC TGCAGCTGGA TCCGCCTCAG AAGGAGCAGC TGGGGAGTGC TCTTGCCGAG 
ATGGACCGAC AGCTCAGGAG GCTGGCAGAC ACCCCGTGGC TCTGCCAGTC CGCAGAGCCC 
GGCGACGAAG AGAGTGTGAT GCTGGATCTT GCCAAGCGCA GTCGCAGTGG TAAATTCCGC 
CTCGTGACCA AGTTTAAAAA GGAGAAAAAC AACAAGAACA AAGAAGCTCA CAGTAGCCTG 
GGAGCCCCGG TTCACCTCTG GGGGACAGAG GAGGTTGCTG CCTGGCTGGA GCACCTCAGT 
CTCTGTGAGT ATAAGGACAT CTTCACACGG CACGACATCC GGGGCTCTGA GCTCCTGCAC 
CTGGAGCGGA GGGACCTCAA GGACCTGGGC GTGACCAAGG TGGGCCACAT GAAGAGGATC 
CTGTGTGGCA TCAAGGAGCT GAGCCGCAGC GCCCCCGCCG TCGAGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510334.1
CDS	80..3550
Translation	MLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_011512032.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512032.3

ORF Insert Sequence:

ATGCTGACCA AACAGAACAA TTCATTCCAG CGATCAAAAA GGAGATACTT TAAGCTTCGA 
GGGCGAACGC TTTACTATGC CAAAACGGCA AAGTCAATCA TATTTGATGA GGTGGATCTG 
ACAGATGCCA GCGTAGCTGA ATCCAGTACC AAAAACGTCA ACAACAGTTT TACGGTCATA 
ACTCCATGCA GGAAGCTCAT CTTGTGTGCT GATAACAGAA AAGAAATGGA AGATTGGATT 
GCAGCATTAA AGACTGTGCA GAACAGGGAG CACTTTGAGC CCACCCAGTA CAGCATGGAC 
CACTTCTCAG GGATGCACAA TTGGTACGCC TGTTCCCACG CGAGGCCGAC CTACTGCAAT 
GTGTGCCGTG AGGCTCTGTC TGGGGTCACG TCGCACGGGC TGTCCTGCGA GGTGTGCAAA 
TTTAAGGCCC ACAAGCGCTG TGCTGTGCGT GCAACCAATA ACTGCAAGTG GACCACACTG 
GCCTCGATCG GGAAGGACAT CATTGAAGAT GCAGATGGGA TTGCAATGCC CCACCAGTGG 
TTGGAAGGAA ACCTACCTGT GAGCGCCAAG TGCACTGTGT GCGACAAGAC CTGTGGCAGT 
GTGCTGCGCC TGCAGGACTG GCGCTGCCTC TGGTGCAAGG CCATGGTTCA CACATCGTGT 
AAAGAATCCT TGCTGACCAA GTGCCCACTT GGCCTGTGCA AAGTGTCAGT CATCCCACCC 
ACGGCTCTCA ACAGCATCGA CTCCGATGGG TTCTGGAAGG CCAGCTGTCC TCCTTCTTGC 
ACAAGCCCAC TGTTGGTCTT CGTCAATTCA AAAAGTGGGG ACAACCAGGG TGTGAAGTTC 
CTCAGAAGAT TCAAACAGCT ACTAAACCCC GCCCAGGTCT TCGACCTCAT GAACGGAGGC 
CCACACCTCG GCTTACGGTT ATTCCAGAAG TTTGACACAT TCCGGATTCT GGTTTGTGGC 
GGGGATGGAA GTGTTGGCTG GGTCCTCTCC GAAATCGACA GCCTCAACCT TCATAAACAG 
TGTCAGCTGG GAGTGCTGCC GCTCGGCACA GGGAACGACT TGGCCCGAGT ACTGGGCTGG 
GGCTCAGCCT GCGATGACGA CACCCAGCTC CCCCAGATCT TGGAGAAGTT GGAGAGAGCC 
AGCACCAAGA TGCTGGACAG GTGGAGCGTC ATGGCATACG AGGCCAAGCT CCCCCGGCAG 
GCCTCCTCCT CTACCGTCAC CGAAGACTTC AGCGAGGATT CCGAGGTACA GCAGATTCTC 
TTCTATGAAG ACTCGGTTGC AGCCCACCTT TCTAAAATCC TCACCTCGGA CCAGCACTCG 
GTGGTCATCT CCTCGGCCAA AGTGCTCTGT GAGACGGTGA AGGACTTCGT GGCACGGGTG 
GGGAAGGCCT ATGAGAAGAC GACCGAGAGC TCGGAGGAGT CAGAGGTCAT GGCCAAGAAG 
TGCTCTGTCC TGAAAGAGAA GCTGGATTCC CTTCTCAAGA CCTTGGACGA TGAGTCCCAG 
GCCTCGTCCT CTCTGCCCAA CCCGCCCCCC ACCATTGCCG AGGAGGCTGA AGATGGAGAT 
GGGTCGGGCA GCATCTGCGG TTCCACCGGA GACCGCTTGG TGGCATCAGC TTGCCCGGCC 
CGGCCGCAGA TATTCCGGCC TCGAGAACAG CTCATGCTGA GAGCCAACAG CCTGAAGAAA 
GCAATTCGTC AGATCATAGA ACACACAGAA AAAGCTGTCG ATGAGCAGAA TGCCCAGACC 
CAGGAGCAGG AGGGCTTCGT CCTGGGCCTC TCTGAGTCAG AGGAGAAGAT GGACCACAGA 
GTGTGCCCAC CACTGTCCCA CAGCGAGAGC TTCGGGGTCC CCAAGGGGAG GAGCCAGCGC 
AAAGTGTCGA AATCTCCGTG TGAAAAGCTG ATCAGCAAAG GGAGTCTGTC CCTAGGCAGT 
TCTGCTTCCC TTCCGCCCCA GCCGGGAAGC CGGGACGGCC TGCCTGCGCT CAACACCAAG 
ATCCTGTACC CAAATGTCCG GGCTGGAATG TCTGGTTCCT TACCCGGTGG CTCAGTCATC 
AGTCGCCTGT TAATTAATGC TGATCCCTTC AACTCTGAAC CAGAAACCCT AGAGTATTAC 
ACGGAGAAAT GTGTCATGAA CAACTATTTT GGCATTGGCC TGGATGCGAA GATATCCCTG 
GACTTTAACA ACAAGCGCGA TGAGCACCCA GAGAAGTGCA GGAGCCGAAC CAAGAACATG 
ATGTGGTATG GAGTTCTTGG AACCAAAGAG TTGCTGCACA GAACCTACAA GAACCTGGAG 
CAAAAGGTCT TGCTGGAGTG TGACGGGCGA CCCATCCCAC TCCCCAGTCT TCAGGGAATT 
GCTGTCCTTA ACATTCCCAG CTATGCCGGA GGAACCAACT TCTGGGGGGG TACCAAGGAA 
GATGATACTT TCGCAGCTCC ATCATTCGAT GACAAGATTC TGGAGGTGGT CGCCGTGTTC 
GGCAGCATGC AGATGGCCGT CTCTCGAGTC ATCAGGCTAC AGCATCATCG GATCGCCCAG 
TGTCGCACGG TGAAGATCTC CATCCTTGGG GATGAGGGCG TGCCTGTGCA GGTGGACGGA 
GAGGCCTGGG TCCAGCCGCC AGGGTACATT CGGATTGTCC ACAAGAACCG GGCACAGACA 
CTGACCAGAG ACAGGGCATT TGAGAGCACC CTGAAGTCCT GGGAAGACAA GCAGAAGTGC 
GAGCTGCCCC GCCCTCCATC CTGTTCCCTG CACCCGGAGA TGCTGTCCGA GGAGGAGGCC 
ACCCAGATGG ACCAGTTTGG GCAGGCAGCA GGGGTCCTCA TTCACAGTAT CCGAGAAATA 
GCTCAGTCTC ACCGGGACAT GGAGCAGGAA CTGGCCCACG CCGTCAATGC CAGCTCCAAG 
TCCATGGACC GTGTGTATGG CAAGCCCAGA ACCACAGAGG GGCTCAACTG CAGCTTCGTC 
CTGGAAATGG TGAATAACTT CAGAGCTCTG CGCAGTGAGA CGGAGCTGCT GCTGTCTGGG 
AAGATGGCCC TGCAGCTGGA TCCGCCTCAG AAGGAGCAGC TGGGGAGTGC TCTTGCCGAG 
ATGGACCGAC AGCTCAGGAG GCTGGCAGAC ACCCCGTGGC TCTGCCAGTC CGCAGAGCCC 
GGCGACGAAG AGAGTGTGAT GCTGGATCTT GCCAAGCGCA GTCGCAGTGG TAAATTCCGC 
CTCGTGACCA AGTTTAAAAA GGAGAAAAAC AACAAGAACA AAGAAGCTCA CAGTAGCCTG 
GGAGCCCCGG TTCACCTCTG GGGGACAGAG GAGGTTGCTG CCTGGCTGGA GCACCTCAGT 
CTCTGTGAGT ATAAGGACAT CTTCACACGG CACGACATCC GGGGCTCTGA GCTCCTGCAC 
CTGGAGCGGA GGGACCTCAA GGACCTGGGC GTGACCAAGG TGGGCCACAT GAAGAGGATC 
CTGTGTGGCA TCAAGGAGCT GAGCCGCAGC GCCCCCGCCG TCGAGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512032.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510334.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X12, mRNA.

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CloneID	OHu61926
Clone ID Related Accession (Same CDS sequence)	XM_011512032.3 , XM_011512032.2 , XM_011512031.2 , XM_011512031.3
Accession Version	XM_011512032.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512032.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACCA AACAGAACAA TTCATTCCAG CGATCAAAAA GGAGATACTT TAAGCTTCGA 
GGGCGAACGC TTTACTATGC CAAAACGGCA AAGTCAATCA TATTTGATGA GGTGGATCTG 
ACAGATGCCA GCGTAGCTGA ATCCAGTACC AAAAACGTCA ACAACAGTTT TACGGTCATA 
ACTCCATGCA GGAAGCTCAT CTTGTGTGCT GATAACAGAA AAGAAATGGA AGATTGGATT 
GCAGCATTAA AGACTGTGCA GAACAGGGAG CACTTTGAGC CCACCCAGTA CAGCATGGAC 
CACTTCTCAG GGATGCACAA TTGGTACGCC TGTTCCCACG CGAGGCCGAC CTACTGCAAT 
GTGTGCCGTG AGGCTCTGTC TGGGGTCACG TCGCACGGGC TGTCCTGCGA GGTGTGCAAA 
TTTAAGGCCC ACAAGCGCTG TGCTGTGCGT GCAACCAATA ACTGCAAGTG GACCACACTG 
GCCTCGATCG GGAAGGACAT CATTGAAGAT GCAGATGGGA TTGCAATGCC CCACCAGTGG 
TTGGAAGGAA ACCTACCTGT GAGCGCCAAG TGCACTGTGT GCGACAAGAC CTGTGGCAGT 
GTGCTGCGCC TGCAGGACTG GCGCTGCCTC TGGTGCAAGG CCATGGTTCA CACATCGTGT 
AAAGAATCCT TGCTGACCAA GTGCCCACTT GGCCTGTGCA AAGTGTCAGT CATCCCACCC 
ACGGCTCTCA ACAGCATCGA CTCCGATGGG TTCTGGAAGG CCAGCTGTCC TCCTTCTTGC 
ACAAGCCCAC TGTTGGTCTT CGTCAATTCA AAAAGTGGGG ACAACCAGGG TGTGAAGTTC 
CTCAGAAGAT TCAAACAGCT ACTAAACCCC GCCCAGGTCT TCGACCTCAT GAACGGAGGC 
CCACACCTCG GCTTACGGTT ATTCCAGAAG TTTGACACAT TCCGGATTCT GGTTTGTGGC 
GGGGATGGAA GTGTTGGCTG GGTCCTCTCC GAAATCGACA GCCTCAACCT TCATAAACAG 
TGTCAGCTGG GAGTGCTGCC GCTCGGCACA GGGAACGACT TGGCCCGAGT ACTGGGCTGG 
GGCTCAGCCT GCGATGACGA CACCCAGCTC CCCCAGATCT TGGAGAAGTT GGAGAGAGCC 
AGCACCAAGA TGCTGGACAG GTGGAGCGTC ATGGCATACG AGGCCAAGCT CCCCCGGCAG 
GCCTCCTCCT CTACCGTCAC CGAAGACTTC AGCGAGGATT CCGAGGTACA GCAGATTCTC 
TTCTATGAAG ACTCGGTTGC AGCCCACCTT TCTAAAATCC TCACCTCGGA CCAGCACTCG 
GTGGTCATCT CCTCGGCCAA AGTGCTCTGT GAGACGGTGA AGGACTTCGT GGCACGGGTG 
GGGAAGGCCT ATGAGAAGAC GACCGAGAGC TCGGAGGAGT CAGAGGTCAT GGCCAAGAAG 
TGCTCTGTCC TGAAAGAGAA GCTGGATTCC CTTCTCAAGA CCTTGGACGA TGAGTCCCAG 
GCCTCGTCCT CTCTGCCCAA CCCGCCCCCC ACCATTGCCG AGGAGGCTGA AGATGGAGAT 
GGGTCGGGCA GCATCTGCGG TTCCACCGGA GACCGCTTGG TGGCATCAGC TTGCCCGGCC 
CGGCCGCAGA TATTCCGGCC TCGAGAACAG CTCATGCTGA GAGCCAACAG CCTGAAGAAA 
GCAATTCGTC AGATCATAGA ACACACAGAA AAAGCTGTCG ATGAGCAGAA TGCCCAGACC 
CAGGAGCAGG AGGGCTTCGT CCTGGGCCTC TCTGAGTCAG AGGAGAAGAT GGACCACAGA 
GTGTGCCCAC CACTGTCCCA CAGCGAGAGC TTCGGGGTCC CCAAGGGGAG GAGCCAGCGC 
AAAGTGTCGA AATCTCCGTG TGAAAAGCTG ATCAGCAAAG GGAGTCTGTC CCTAGGCAGT 
TCTGCTTCCC TTCCGCCCCA GCCGGGAAGC CGGGACGGCC TGCCTGCGCT CAACACCAAG 
ATCCTGTACC CAAATGTCCG GGCTGGAATG TCTGGTTCCT TACCCGGTGG CTCAGTCATC 
AGTCGCCTGT TAATTAATGC TGATCCCTTC AACTCTGAAC CAGAAACCCT AGAGTATTAC 
ACGGAGAAAT GTGTCATGAA CAACTATTTT GGCATTGGCC TGGATGCGAA GATATCCCTG 
GACTTTAACA ACAAGCGCGA TGAGCACCCA GAGAAGTGCA GGAGCCGAAC CAAGAACATG 
ATGTGGTATG GAGTTCTTGG AACCAAAGAG TTGCTGCACA GAACCTACAA GAACCTGGAG 
CAAAAGGTCT TGCTGGAGTG TGACGGGCGA CCCATCCCAC TCCCCAGTCT TCAGGGAATT 
GCTGTCCTTA ACATTCCCAG CTATGCCGGA GGAACCAACT TCTGGGGGGG TACCAAGGAA 
GATGATACTT TCGCAGCTCC ATCATTCGAT GACAAGATTC TGGAGGTGGT CGCCGTGTTC 
GGCAGCATGC AGATGGCCGT CTCTCGAGTC ATCAGGCTAC AGCATCATCG GATCGCCCAG 
TGTCGCACGG TGAAGATCTC CATCCTTGGG GATGAGGGCG TGCCTGTGCA GGTGGACGGA 
GAGGCCTGGG TCCAGCCGCC AGGGTACATT CGGATTGTCC ACAAGAACCG GGCACAGACA 
CTGACCAGAG ACAGGGCATT TGAGAGCACC CTGAAGTCCT GGGAAGACAA GCAGAAGTGC 
GAGCTGCCCC GCCCTCCATC CTGTTCCCTG CACCCGGAGA TGCTGTCCGA GGAGGAGGCC 
ACCCAGATGG ACCAGTTTGG GCAGGCAGCA GGGGTCCTCA TTCACAGTAT CCGAGAAATA 
GCTCAGTCTC ACCGGGACAT GGAGCAGGAA CTGGCCCACG CCGTCAATGC CAGCTCCAAG 
TCCATGGACC GTGTGTATGG CAAGCCCAGA ACCACAGAGG GGCTCAACTG CAGCTTCGTC 
CTGGAAATGG TGAATAACTT CAGAGCTCTG CGCAGTGAGA CGGAGCTGCT GCTGTCTGGG 
AAGATGGCCC TGCAGCTGGA TCCGCCTCAG AAGGAGCAGC TGGGGAGTGC TCTTGCCGAG 
ATGGACCGAC AGCTCAGGAG GCTGGCAGAC ACCCCGTGGC TCTGCCAGTC CGCAGAGCCC 
GGCGACGAAG AGAGTGTGAT GCTGGATCTT GCCAAGCGCA GTCGCAGTGG TAAATTCCGC 
CTCGTGACCA AGTTTAAAAA GGAGAAAAAC AACAAGAACA AAGAAGCTCA CAGTAGCCTG 
GGAGCCCCGG TTCACCTCTG GGGGACAGAG GAGGTTGCTG CCTGGCTGGA GCACCTCAGT 
CTCTGTGAGT ATAAGGACAT CTTCACACGG CACGACATCC GGGGCTCTGA GCTCCTGCAC 
CTGGAGCGGA GGGACCTCAA GGACCTGGGC GTGACCAAGG TGGGCCACAT GAAGAGGATC 
CTGTGTGGCA TCAAGGAGCT GAGCCGCAGC GCCCCCGCCG TCGAGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510334.1
CDS	101..3571
Translation	MLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_011512032.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512032.2

ORF Insert Sequence:

ATGCTGACCA AACAGAACAA TTCATTCCAG CGATCAAAAA GGAGATACTT TAAGCTTCGA 
GGGCGAACGC TTTACTATGC CAAAACGGCA AAGTCAATCA TATTTGATGA GGTGGATCTG 
ACAGATGCCA GCGTAGCTGA ATCCAGTACC AAAAACGTCA ACAACAGTTT TACGGTCATA 
ACTCCATGCA GGAAGCTCAT CTTGTGTGCT GATAACAGAA AAGAAATGGA AGATTGGATT 
GCAGCATTAA AGACTGTGCA GAACAGGGAG CACTTTGAGC CCACCCAGTA CAGCATGGAC 
CACTTCTCAG GGATGCACAA TTGGTACGCC TGTTCCCACG CGAGGCCGAC CTACTGCAAT 
GTGTGCCGTG AGGCTCTGTC TGGGGTCACG TCGCACGGGC TGTCCTGCGA GGTGTGCAAA 
TTTAAGGCCC ACAAGCGCTG TGCTGTGCGT GCAACCAATA ACTGCAAGTG GACCACACTG 
GCCTCGATCG GGAAGGACAT CATTGAAGAT GCAGATGGGA TTGCAATGCC CCACCAGTGG 
TTGGAAGGAA ACCTACCTGT GAGCGCCAAG TGCACTGTGT GCGACAAGAC CTGTGGCAGT 
GTGCTGCGCC TGCAGGACTG GCGCTGCCTC TGGTGCAAGG CCATGGTTCA CACATCGTGT 
AAAGAATCCT TGCTGACCAA GTGCCCACTT GGCCTGTGCA AAGTGTCAGT CATCCCACCC 
ACGGCTCTCA ACAGCATCGA CTCCGATGGG TTCTGGAAGG CCAGCTGTCC TCCTTCTTGC 
ACAAGCCCAC TGTTGGTCTT CGTCAATTCA AAAAGTGGGG ACAACCAGGG TGTGAAGTTC 
CTCAGAAGAT TCAAACAGCT ACTAAACCCC GCCCAGGTCT TCGACCTCAT GAACGGAGGC 
CCACACCTCG GCTTACGGTT ATTCCAGAAG TTTGACACAT TCCGGATTCT GGTTTGTGGC 
GGGGATGGAA GTGTTGGCTG GGTCCTCTCC GAAATCGACA GCCTCAACCT TCATAAACAG 
TGTCAGCTGG GAGTGCTGCC GCTCGGCACA GGGAACGACT TGGCCCGAGT ACTGGGCTGG 
GGCTCAGCCT GCGATGACGA CACCCAGCTC CCCCAGATCT TGGAGAAGTT GGAGAGAGCC 
AGCACCAAGA TGCTGGACAG GTGGAGCGTC ATGGCATACG AGGCCAAGCT CCCCCGGCAG 
GCCTCCTCCT CTACCGTCAC CGAAGACTTC AGCGAGGATT CCGAGGTACA GCAGATTCTC 
TTCTATGAAG ACTCGGTTGC AGCCCACCTT TCTAAAATCC TCACCTCGGA CCAGCACTCG 
GTGGTCATCT CCTCGGCCAA AGTGCTCTGT GAGACGGTGA AGGACTTCGT GGCACGGGTG 
GGGAAGGCCT ATGAGAAGAC GACCGAGAGC TCGGAGGAGT CAGAGGTCAT GGCCAAGAAG 
TGCTCTGTCC TGAAAGAGAA GCTGGATTCC CTTCTCAAGA CCTTGGACGA TGAGTCCCAG 
GCCTCGTCCT CTCTGCCCAA CCCGCCCCCC ACCATTGCCG AGGAGGCTGA AGATGGAGAT 
GGGTCGGGCA GCATCTGCGG TTCCACCGGA GACCGCTTGG TGGCATCAGC TTGCCCGGCC 
CGGCCGCAGA TATTCCGGCC TCGAGAACAG CTCATGCTGA GAGCCAACAG CCTGAAGAAA 
GCAATTCGTC AGATCATAGA ACACACAGAA AAAGCTGTCG ATGAGCAGAA TGCCCAGACC 
CAGGAGCAGG AGGGCTTCGT CCTGGGCCTC TCTGAGTCAG AGGAGAAGAT GGACCACAGA 
GTGTGCCCAC CACTGTCCCA CAGCGAGAGC TTCGGGGTCC CCAAGGGGAG GAGCCAGCGC 
AAAGTGTCGA AATCTCCGTG TGAAAAGCTG ATCAGCAAAG GGAGTCTGTC CCTAGGCAGT 
TCTGCTTCCC TTCCGCCCCA GCCGGGAAGC CGGGACGGCC TGCCTGCGCT CAACACCAAG 
ATCCTGTACC CAAATGTCCG GGCTGGAATG TCTGGTTCCT TACCCGGTGG CTCAGTCATC 
AGTCGCCTGT TAATTAATGC TGATCCCTTC AACTCTGAAC CAGAAACCCT AGAGTATTAC 
ACGGAGAAAT GTGTCATGAA CAACTATTTT GGCATTGGCC TGGATGCGAA GATATCCCTG 
GACTTTAACA ACAAGCGCGA TGAGCACCCA GAGAAGTGCA GGAGCCGAAC CAAGAACATG 
ATGTGGTATG GAGTTCTTGG AACCAAAGAG TTGCTGCACA GAACCTACAA GAACCTGGAG 
CAAAAGGTCT TGCTGGAGTG TGACGGGCGA CCCATCCCAC TCCCCAGTCT TCAGGGAATT 
GCTGTCCTTA ACATTCCCAG CTATGCCGGA GGAACCAACT TCTGGGGGGG TACCAAGGAA 
GATGATACTT TCGCAGCTCC ATCATTCGAT GACAAGATTC TGGAGGTGGT CGCCGTGTTC 
GGCAGCATGC AGATGGCCGT CTCTCGAGTC ATCAGGCTAC AGCATCATCG GATCGCCCAG 
TGTCGCACGG TGAAGATCTC CATCCTTGGG GATGAGGGCG TGCCTGTGCA GGTGGACGGA 
GAGGCCTGGG TCCAGCCGCC AGGGTACATT CGGATTGTCC ACAAGAACCG GGCACAGACA 
CTGACCAGAG ACAGGGCATT TGAGAGCACC CTGAAGTCCT GGGAAGACAA GCAGAAGTGC 
GAGCTGCCCC GCCCTCCATC CTGTTCCCTG CACCCGGAGA TGCTGTCCGA GGAGGAGGCC 
ACCCAGATGG ACCAGTTTGG GCAGGCAGCA GGGGTCCTCA TTCACAGTAT CCGAGAAATA 
GCTCAGTCTC ACCGGGACAT GGAGCAGGAA CTGGCCCACG CCGTCAATGC CAGCTCCAAG 
TCCATGGACC GTGTGTATGG CAAGCCCAGA ACCACAGAGG GGCTCAACTG CAGCTTCGTC 
CTGGAAATGG TGAATAACTT CAGAGCTCTG CGCAGTGAGA CGGAGCTGCT GCTGTCTGGG 
AAGATGGCCC TGCAGCTGGA TCCGCCTCAG AAGGAGCAGC TGGGGAGTGC TCTTGCCGAG 
ATGGACCGAC AGCTCAGGAG GCTGGCAGAC ACCCCGTGGC TCTGCCAGTC CGCAGAGCCC 
GGCGACGAAG AGAGTGTGAT GCTGGATCTT GCCAAGCGCA GTCGCAGTGG TAAATTCCGC 
CTCGTGACCA AGTTTAAAAA GGAGAAAAAC AACAAGAACA AAGAAGCTCA CAGTAGCCTG 
GGAGCCCCGG TTCACCTCTG GGGGACAGAG GAGGTTGCTG CCTGGCTGGA GCACCTCAGT 
CTCTGTGAGT ATAAGGACAT CTTCACACGG CACGACATCC GGGGCTCTGA GCTCCTGCAC 
CTGGAGCGGA GGGACCTCAA GGACCTGGGC GTGACCAAGG TGGGCCACAT GAAGAGGATC 
CTGTGTGGCA TCAAGGAGCT GAGCCGCAGC GCCCCCGCCG TCGAGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512031.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510333.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X5, mRNA.

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CloneID	OHu61926
Clone ID Related Accession (Same CDS sequence)	XM_011512032.3 , XM_011512032.2 , XM_011512031.2 , XM_011512031.3
Accession Version	XM_011512031.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512031.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACCA AACAGAACAA TTCATTCCAG CGATCAAAAA GGAGATACTT TAAGCTTCGA 
GGGCGAACGC TTTACTATGC CAAAACGGCA AAGTCAATCA TATTTGATGA GGTGGATCTG 
ACAGATGCCA GCGTAGCTGA ATCCAGTACC AAAAACGTCA ACAACAGTTT TACGGTCATA 
ACTCCATGCA GGAAGCTCAT CTTGTGTGCT GATAACAGAA AAGAAATGGA AGATTGGATT 
GCAGCATTAA AGACTGTGCA GAACAGGGAG CACTTTGAGC CCACCCAGTA CAGCATGGAC 
CACTTCTCAG GGATGCACAA TTGGTACGCC TGTTCCCACG CGAGGCCGAC CTACTGCAAT 
GTGTGCCGTG AGGCTCTGTC TGGGGTCACG TCGCACGGGC TGTCCTGCGA GGTGTGCAAA 
TTTAAGGCCC ACAAGCGCTG TGCTGTGCGT GCAACCAATA ACTGCAAGTG GACCACACTG 
GCCTCGATCG GGAAGGACAT CATTGAAGAT GCAGATGGGA TTGCAATGCC CCACCAGTGG 
TTGGAAGGAA ACCTACCTGT GAGCGCCAAG TGCACTGTGT GCGACAAGAC CTGTGGCAGT 
GTGCTGCGCC TGCAGGACTG GCGCTGCCTC TGGTGCAAGG CCATGGTTCA CACATCGTGT 
AAAGAATCCT TGCTGACCAA GTGCCCACTT GGCCTGTGCA AAGTGTCAGT CATCCCACCC 
ACGGCTCTCA ACAGCATCGA CTCCGATGGG TTCTGGAAGG CCAGCTGTCC TCCTTCTTGC 
ACAAGCCCAC TGTTGGTCTT CGTCAATTCA AAAAGTGGGG ACAACCAGGG TGTGAAGTTC 
CTCAGAAGAT TCAAACAGCT ACTAAACCCC GCCCAGGTCT TCGACCTCAT GAACGGAGGC 
CCACACCTCG GCTTACGGTT ATTCCAGAAG TTTGACACAT TCCGGATTCT GGTTTGTGGC 
GGGGATGGAA GTGTTGGCTG GGTCCTCTCC GAAATCGACA GCCTCAACCT TCATAAACAG 
TGTCAGCTGG GAGTGCTGCC GCTCGGCACA GGGAACGACT TGGCCCGAGT ACTGGGCTGG 
GGCTCAGCCT GCGATGACGA CACCCAGCTC CCCCAGATCT TGGAGAAGTT GGAGAGAGCC 
AGCACCAAGA TGCTGGACAG GTGGAGCGTC ATGGCATACG AGGCCAAGCT CCCCCGGCAG 
GCCTCCTCCT CTACCGTCAC CGAAGACTTC AGCGAGGATT CCGAGGTACA GCAGATTCTC 
TTCTATGAAG ACTCGGTTGC AGCCCACCTT TCTAAAATCC TCACCTCGGA CCAGCACTCG 
GTGGTCATCT CCTCGGCCAA AGTGCTCTGT GAGACGGTGA AGGACTTCGT GGCACGGGTG 
GGGAAGGCCT ATGAGAAGAC GACCGAGAGC TCGGAGGAGT CAGAGGTCAT GGCCAAGAAG 
TGCTCTGTCC TGAAAGAGAA GCTGGATTCC CTTCTCAAGA CCTTGGACGA TGAGTCCCAG 
GCCTCGTCCT CTCTGCCCAA CCCGCCCCCC ACCATTGCCG AGGAGGCTGA AGATGGAGAT 
GGGTCGGGCA GCATCTGCGG TTCCACCGGA GACCGCTTGG TGGCATCAGC TTGCCCGGCC 
CGGCCGCAGA TATTCCGGCC TCGAGAACAG CTCATGCTGA GAGCCAACAG CCTGAAGAAA 
GCAATTCGTC AGATCATAGA ACACACAGAA AAAGCTGTCG ATGAGCAGAA TGCCCAGACC 
CAGGAGCAGG AGGGCTTCGT CCTGGGCCTC TCTGAGTCAG AGGAGAAGAT GGACCACAGA 
GTGTGCCCAC CACTGTCCCA CAGCGAGAGC TTCGGGGTCC CCAAGGGGAG GAGCCAGCGC 
AAAGTGTCGA AATCTCCGTG TGAAAAGCTG ATCAGCAAAG GGAGTCTGTC CCTAGGCAGT 
TCTGCTTCCC TTCCGCCCCA GCCGGGAAGC CGGGACGGCC TGCCTGCGCT CAACACCAAG 
ATCCTGTACC CAAATGTCCG GGCTGGAATG TCTGGTTCCT TACCCGGTGG CTCAGTCATC 
AGTCGCCTGT TAATTAATGC TGATCCCTTC AACTCTGAAC CAGAAACCCT AGAGTATTAC 
ACGGAGAAAT GTGTCATGAA CAACTATTTT GGCATTGGCC TGGATGCGAA GATATCCCTG 
GACTTTAACA ACAAGCGCGA TGAGCACCCA GAGAAGTGCA GGAGCCGAAC CAAGAACATG 
ATGTGGTATG GAGTTCTTGG AACCAAAGAG TTGCTGCACA GAACCTACAA GAACCTGGAG 
CAAAAGGTCT TGCTGGAGTG TGACGGGCGA CCCATCCCAC TCCCCAGTCT TCAGGGAATT 
GCTGTCCTTA ACATTCCCAG CTATGCCGGA GGAACCAACT TCTGGGGGGG TACCAAGGAA 
GATGATACTT TCGCAGCTCC ATCATTCGAT GACAAGATTC TGGAGGTGGT CGCCGTGTTC 
GGCAGCATGC AGATGGCCGT CTCTCGAGTC ATCAGGCTAC AGCATCATCG GATCGCCCAG 
TGTCGCACGG TGAAGATCTC CATCCTTGGG GATGAGGGCG TGCCTGTGCA GGTGGACGGA 
GAGGCCTGGG TCCAGCCGCC AGGGTACATT CGGATTGTCC ACAAGAACCG GGCACAGACA 
CTGACCAGAG ACAGGGCATT TGAGAGCACC CTGAAGTCCT GGGAAGACAA GCAGAAGTGC 
GAGCTGCCCC GCCCTCCATC CTGTTCCCTG CACCCGGAGA TGCTGTCCGA GGAGGAGGCC 
ACCCAGATGG ACCAGTTTGG GCAGGCAGCA GGGGTCCTCA TTCACAGTAT CCGAGAAATA 
GCTCAGTCTC ACCGGGACAT GGAGCAGGAA CTGGCCCACG CCGTCAATGC CAGCTCCAAG 
TCCATGGACC GTGTGTATGG CAAGCCCAGA ACCACAGAGG GGCTCAACTG CAGCTTCGTC 
CTGGAAATGG TGAATAACTT CAGAGCTCTG CGCAGTGAGA CGGAGCTGCT GCTGTCTGGG 
AAGATGGCCC TGCAGCTGGA TCCGCCTCAG AAGGAGCAGC TGGGGAGTGC TCTTGCCGAG 
ATGGACCGAC AGCTCAGGAG GCTGGCAGAC ACCCCGTGGC TCTGCCAGTC CGCAGAGCCC 
GGCGACGAAG AGAGTGTGAT GCTGGATCTT GCCAAGCGCA GTCGCAGTGG TAAATTCCGC 
CTCGTGACCA AGTTTAAAAA GGAGAAAAAC AACAAGAACA AAGAAGCTCA CAGTAGCCTG 
GGAGCCCCGG TTCACCTCTG GGGGACAGAG GAGGTTGCTG CCTGGCTGGA GCACCTCAGT 
CTCTGTGAGT ATAAGGACAT CTTCACACGG CACGACATCC GGGGCTCTGA GCTCCTGCAC 
CTGGAGCGGA GGGACCTCAA GGACCTGGGC GTGACCAAGG TGGGCCACAT GAAGAGGATC 
CTGTGTGGCA TCAAGGAGCT GAGCCGCAGC GCCCCCGCCG TCGAGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510333.1
CDS	117..3587
Translation	MLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_011512031.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512031.2

ORF Insert Sequence:

ATGCTGACCA AACAGAACAA TTCATTCCAG CGATCAAAAA GGAGATACTT TAAGCTTCGA 
GGGCGAACGC TTTACTATGC CAAAACGGCA AAGTCAATCA TATTTGATGA GGTGGATCTG 
ACAGATGCCA GCGTAGCTGA ATCCAGTACC AAAAACGTCA ACAACAGTTT TACGGTCATA 
ACTCCATGCA GGAAGCTCAT CTTGTGTGCT GATAACAGAA AAGAAATGGA AGATTGGATT 
GCAGCATTAA AGACTGTGCA GAACAGGGAG CACTTTGAGC CCACCCAGTA CAGCATGGAC 
CACTTCTCAG GGATGCACAA TTGGTACGCC TGTTCCCACG CGAGGCCGAC CTACTGCAAT 
GTGTGCCGTG AGGCTCTGTC TGGGGTCACG TCGCACGGGC TGTCCTGCGA GGTGTGCAAA 
TTTAAGGCCC ACAAGCGCTG TGCTGTGCGT GCAACCAATA ACTGCAAGTG GACCACACTG 
GCCTCGATCG GGAAGGACAT CATTGAAGAT GCAGATGGGA TTGCAATGCC CCACCAGTGG 
TTGGAAGGAA ACCTACCTGT GAGCGCCAAG TGCACTGTGT GCGACAAGAC CTGTGGCAGT 
GTGCTGCGCC TGCAGGACTG GCGCTGCCTC TGGTGCAAGG CCATGGTTCA CACATCGTGT 
AAAGAATCCT TGCTGACCAA GTGCCCACTT GGCCTGTGCA AAGTGTCAGT CATCCCACCC 
ACGGCTCTCA ACAGCATCGA CTCCGATGGG TTCTGGAAGG CCAGCTGTCC TCCTTCTTGC 
ACAAGCCCAC TGTTGGTCTT CGTCAATTCA AAAAGTGGGG ACAACCAGGG TGTGAAGTTC 
CTCAGAAGAT TCAAACAGCT ACTAAACCCC GCCCAGGTCT TCGACCTCAT GAACGGAGGC 
CCACACCTCG GCTTACGGTT ATTCCAGAAG TTTGACACAT TCCGGATTCT GGTTTGTGGC 
GGGGATGGAA GTGTTGGCTG GGTCCTCTCC GAAATCGACA GCCTCAACCT TCATAAACAG 
TGTCAGCTGG GAGTGCTGCC GCTCGGCACA GGGAACGACT TGGCCCGAGT ACTGGGCTGG 
GGCTCAGCCT GCGATGACGA CACCCAGCTC CCCCAGATCT TGGAGAAGTT GGAGAGAGCC 
AGCACCAAGA TGCTGGACAG GTGGAGCGTC ATGGCATACG AGGCCAAGCT CCCCCGGCAG 
GCCTCCTCCT CTACCGTCAC CGAAGACTTC AGCGAGGATT CCGAGGTACA GCAGATTCTC 
TTCTATGAAG ACTCGGTTGC AGCCCACCTT TCTAAAATCC TCACCTCGGA CCAGCACTCG 
GTGGTCATCT CCTCGGCCAA AGTGCTCTGT GAGACGGTGA AGGACTTCGT GGCACGGGTG 
GGGAAGGCCT ATGAGAAGAC GACCGAGAGC TCGGAGGAGT CAGAGGTCAT GGCCAAGAAG 
TGCTCTGTCC TGAAAGAGAA GCTGGATTCC CTTCTCAAGA CCTTGGACGA TGAGTCCCAG 
GCCTCGTCCT CTCTGCCCAA CCCGCCCCCC ACCATTGCCG AGGAGGCTGA AGATGGAGAT 
GGGTCGGGCA GCATCTGCGG TTCCACCGGA GACCGCTTGG TGGCATCAGC TTGCCCGGCC 
CGGCCGCAGA TATTCCGGCC TCGAGAACAG CTCATGCTGA GAGCCAACAG CCTGAAGAAA 
GCAATTCGTC AGATCATAGA ACACACAGAA AAAGCTGTCG ATGAGCAGAA TGCCCAGACC 
CAGGAGCAGG AGGGCTTCGT CCTGGGCCTC TCTGAGTCAG AGGAGAAGAT GGACCACAGA 
GTGTGCCCAC CACTGTCCCA CAGCGAGAGC TTCGGGGTCC CCAAGGGGAG GAGCCAGCGC 
AAAGTGTCGA AATCTCCGTG TGAAAAGCTG ATCAGCAAAG GGAGTCTGTC CCTAGGCAGT 
TCTGCTTCCC TTCCGCCCCA GCCGGGAAGC CGGGACGGCC TGCCTGCGCT CAACACCAAG 
ATCCTGTACC CAAATGTCCG GGCTGGAATG TCTGGTTCCT TACCCGGTGG CTCAGTCATC 
AGTCGCCTGT TAATTAATGC TGATCCCTTC AACTCTGAAC CAGAAACCCT AGAGTATTAC 
ACGGAGAAAT GTGTCATGAA CAACTATTTT GGCATTGGCC TGGATGCGAA GATATCCCTG 
GACTTTAACA ACAAGCGCGA TGAGCACCCA GAGAAGTGCA GGAGCCGAAC CAAGAACATG 
ATGTGGTATG GAGTTCTTGG AACCAAAGAG TTGCTGCACA GAACCTACAA GAACCTGGAG 
CAAAAGGTCT TGCTGGAGTG TGACGGGCGA CCCATCCCAC TCCCCAGTCT TCAGGGAATT 
GCTGTCCTTA ACATTCCCAG CTATGCCGGA GGAACCAACT TCTGGGGGGG TACCAAGGAA 
GATGATACTT TCGCAGCTCC ATCATTCGAT GACAAGATTC TGGAGGTGGT CGCCGTGTTC 
GGCAGCATGC AGATGGCCGT CTCTCGAGTC ATCAGGCTAC AGCATCATCG GATCGCCCAG 
TGTCGCACGG TGAAGATCTC CATCCTTGGG GATGAGGGCG TGCCTGTGCA GGTGGACGGA 
GAGGCCTGGG TCCAGCCGCC AGGGTACATT CGGATTGTCC ACAAGAACCG GGCACAGACA 
CTGACCAGAG ACAGGGCATT TGAGAGCACC CTGAAGTCCT GGGAAGACAA GCAGAAGTGC 
GAGCTGCCCC GCCCTCCATC CTGTTCCCTG CACCCGGAGA TGCTGTCCGA GGAGGAGGCC 
ACCCAGATGG ACCAGTTTGG GCAGGCAGCA GGGGTCCTCA TTCACAGTAT CCGAGAAATA 
GCTCAGTCTC ACCGGGACAT GGAGCAGGAA CTGGCCCACG CCGTCAATGC CAGCTCCAAG 
TCCATGGACC GTGTGTATGG CAAGCCCAGA ACCACAGAGG GGCTCAACTG CAGCTTCGTC 
CTGGAAATGG TGAATAACTT CAGAGCTCTG CGCAGTGAGA CGGAGCTGCT GCTGTCTGGG 
AAGATGGCCC TGCAGCTGGA TCCGCCTCAG AAGGAGCAGC TGGGGAGTGC TCTTGCCGAG 
ATGGACCGAC AGCTCAGGAG GCTGGCAGAC ACCCCGTGGC TCTGCCAGTC CGCAGAGCCC 
GGCGACGAAG AGAGTGTGAT GCTGGATCTT GCCAAGCGCA GTCGCAGTGG TAAATTCCGC 
CTCGTGACCA AGTTTAAAAA GGAGAAAAAC AACAAGAACA AAGAAGCTCA CAGTAGCCTG 
GGAGCCCCGG TTCACCTCTG GGGGACAGAG GAGGTTGCTG CCTGGCTGGA GCACCTCAGT 
CTCTGTGAGT ATAAGGACAT CTTCACACGG CACGACATCC GGGGCTCTGA GCTCCTGCAC 
CTGGAGCGGA GGGACCTCAA GGACCTGGGC GTGACCAAGG TGGGCCACAT GAAGAGGATC 
CTGTGTGGCA TCAAGGAGCT GAGCCGCAGC GCCCCCGCCG TCGAGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512031.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510333.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu61926
Clone ID Related Accession (Same CDS sequence)	XM_011512032.3 , XM_011512032.2 , XM_011512031.2 , XM_011512031.3
Accession Version	XM_011512031.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512031.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACCA AACAGAACAA TTCATTCCAG CGATCAAAAA GGAGATACTT TAAGCTTCGA 
GGGCGAACGC TTTACTATGC CAAAACGGCA AAGTCAATCA TATTTGATGA GGTGGATCTG 
ACAGATGCCA GCGTAGCTGA ATCCAGTACC AAAAACGTCA ACAACAGTTT TACGGTCATA 
ACTCCATGCA GGAAGCTCAT CTTGTGTGCT GATAACAGAA AAGAAATGGA AGATTGGATT 
GCAGCATTAA AGACTGTGCA GAACAGGGAG CACTTTGAGC CCACCCAGTA CAGCATGGAC 
CACTTCTCAG GGATGCACAA TTGGTACGCC TGTTCCCACG CGAGGCCGAC CTACTGCAAT 
GTGTGCCGTG AGGCTCTGTC TGGGGTCACG TCGCACGGGC TGTCCTGCGA GGTGTGCAAA 
TTTAAGGCCC ACAAGCGCTG TGCTGTGCGT GCAACCAATA ACTGCAAGTG GACCACACTG 
GCCTCGATCG GGAAGGACAT CATTGAAGAT GCAGATGGGA TTGCAATGCC CCACCAGTGG 
TTGGAAGGAA ACCTACCTGT GAGCGCCAAG TGCACTGTGT GCGACAAGAC CTGTGGCAGT 
GTGCTGCGCC TGCAGGACTG GCGCTGCCTC TGGTGCAAGG CCATGGTTCA CACATCGTGT 
AAAGAATCCT TGCTGACCAA GTGCCCACTT GGCCTGTGCA AAGTGTCAGT CATCCCACCC 
ACGGCTCTCA ACAGCATCGA CTCCGATGGG TTCTGGAAGG CCAGCTGTCC TCCTTCTTGC 
ACAAGCCCAC TGTTGGTCTT CGTCAATTCA AAAAGTGGGG ACAACCAGGG TGTGAAGTTC 
CTCAGAAGAT TCAAACAGCT ACTAAACCCC GCCCAGGTCT TCGACCTCAT GAACGGAGGC 
CCACACCTCG GCTTACGGTT ATTCCAGAAG TTTGACACAT TCCGGATTCT GGTTTGTGGC 
GGGGATGGAA GTGTTGGCTG GGTCCTCTCC GAAATCGACA GCCTCAACCT TCATAAACAG 
TGTCAGCTGG GAGTGCTGCC GCTCGGCACA GGGAACGACT TGGCCCGAGT ACTGGGCTGG 
GGCTCAGCCT GCGATGACGA CACCCAGCTC CCCCAGATCT TGGAGAAGTT GGAGAGAGCC 
AGCACCAAGA TGCTGGACAG GTGGAGCGTC ATGGCATACG AGGCCAAGCT CCCCCGGCAG 
GCCTCCTCCT CTACCGTCAC CGAAGACTTC AGCGAGGATT CCGAGGTACA GCAGATTCTC 
TTCTATGAAG ACTCGGTTGC AGCCCACCTT TCTAAAATCC TCACCTCGGA CCAGCACTCG 
GTGGTCATCT CCTCGGCCAA AGTGCTCTGT GAGACGGTGA AGGACTTCGT GGCACGGGTG 
GGGAAGGCCT ATGAGAAGAC GACCGAGAGC TCGGAGGAGT CAGAGGTCAT GGCCAAGAAG 
TGCTCTGTCC TGAAAGAGAA GCTGGATTCC CTTCTCAAGA CCTTGGACGA TGAGTCCCAG 
GCCTCGTCCT CTCTGCCCAA CCCGCCCCCC ACCATTGCCG AGGAGGCTGA AGATGGAGAT 
GGGTCGGGCA GCATCTGCGG TTCCACCGGA GACCGCTTGG TGGCATCAGC TTGCCCGGCC 
CGGCCGCAGA TATTCCGGCC TCGAGAACAG CTCATGCTGA GAGCCAACAG CCTGAAGAAA 
GCAATTCGTC AGATCATAGA ACACACAGAA AAAGCTGTCG ATGAGCAGAA TGCCCAGACC 
CAGGAGCAGG AGGGCTTCGT CCTGGGCCTC TCTGAGTCAG AGGAGAAGAT GGACCACAGA 
GTGTGCCCAC CACTGTCCCA CAGCGAGAGC TTCGGGGTCC CCAAGGGGAG GAGCCAGCGC 
AAAGTGTCGA AATCTCCGTG TGAAAAGCTG ATCAGCAAAG GGAGTCTGTC CCTAGGCAGT 
TCTGCTTCCC TTCCGCCCCA GCCGGGAAGC CGGGACGGCC TGCCTGCGCT CAACACCAAG 
ATCCTGTACC CAAATGTCCG GGCTGGAATG TCTGGTTCCT TACCCGGTGG CTCAGTCATC 
AGTCGCCTGT TAATTAATGC TGATCCCTTC AACTCTGAAC CAGAAACCCT AGAGTATTAC 
ACGGAGAAAT GTGTCATGAA CAACTATTTT GGCATTGGCC TGGATGCGAA GATATCCCTG 
GACTTTAACA ACAAGCGCGA TGAGCACCCA GAGAAGTGCA GGAGCCGAAC CAAGAACATG 
ATGTGGTATG GAGTTCTTGG AACCAAAGAG TTGCTGCACA GAACCTACAA GAACCTGGAG 
CAAAAGGTCT TGCTGGAGTG TGACGGGCGA CCCATCCCAC TCCCCAGTCT TCAGGGAATT 
GCTGTCCTTA ACATTCCCAG CTATGCCGGA GGAACCAACT TCTGGGGGGG TACCAAGGAA 
GATGATACTT TCGCAGCTCC ATCATTCGAT GACAAGATTC TGGAGGTGGT CGCCGTGTTC 
GGCAGCATGC AGATGGCCGT CTCTCGAGTC ATCAGGCTAC AGCATCATCG GATCGCCCAG 
TGTCGCACGG TGAAGATCTC CATCCTTGGG GATGAGGGCG TGCCTGTGCA GGTGGACGGA 
GAGGCCTGGG TCCAGCCGCC AGGGTACATT CGGATTGTCC ACAAGAACCG GGCACAGACA 
CTGACCAGAG ACAGGGCATT TGAGAGCACC CTGAAGTCCT GGGAAGACAA GCAGAAGTGC 
GAGCTGCCCC GCCCTCCATC CTGTTCCCTG CACCCGGAGA TGCTGTCCGA GGAGGAGGCC 
ACCCAGATGG ACCAGTTTGG GCAGGCAGCA GGGGTCCTCA TTCACAGTAT CCGAGAAATA 
GCTCAGTCTC ACCGGGACAT GGAGCAGGAA CTGGCCCACG CCGTCAATGC CAGCTCCAAG 
TCCATGGACC GTGTGTATGG CAAGCCCAGA ACCACAGAGG GGCTCAACTG CAGCTTCGTC 
CTGGAAATGG TGAATAACTT CAGAGCTCTG CGCAGTGAGA CGGAGCTGCT GCTGTCTGGG 
AAGATGGCCC TGCAGCTGGA TCCGCCTCAG AAGGAGCAGC TGGGGAGTGC TCTTGCCGAG 
ATGGACCGAC AGCTCAGGAG GCTGGCAGAC ACCCCGTGGC TCTGCCAGTC CGCAGAGCCC 
GGCGACGAAG AGAGTGTGAT GCTGGATCTT GCCAAGCGCA GTCGCAGTGG TAAATTCCGC 
CTCGTGACCA AGTTTAAAAA GGAGAAAAAC AACAAGAACA AAGAAGCTCA CAGTAGCCTG 
GGAGCCCCGG TTCACCTCTG GGGGACAGAG GAGGTTGCTG CCTGGCTGGA GCACCTCAGT 
CTCTGTGAGT ATAAGGACAT CTTCACACGG CACGACATCC GGGGCTCTGA GCTCCTGCAC 
CTGGAGCGGA GGGACCTCAA GGACCTGGGC GTGACCAAGG TGGGCCACAT GAAGAGGATC 
CTGTGTGGCA TCAAGGAGCT GAGCCGCAGC GCCCCCGCCG TCGAGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510333.1
CDS	118..3588
Translation	MLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_011512031.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512031.3

ORF Insert Sequence:

ATGCTGACCA AACAGAACAA TTCATTCCAG CGATCAAAAA GGAGATACTT TAAGCTTCGA 
GGGCGAACGC TTTACTATGC CAAAACGGCA AAGTCAATCA TATTTGATGA GGTGGATCTG 
ACAGATGCCA GCGTAGCTGA ATCCAGTACC AAAAACGTCA ACAACAGTTT TACGGTCATA 
ACTCCATGCA GGAAGCTCAT CTTGTGTGCT GATAACAGAA AAGAAATGGA AGATTGGATT 
GCAGCATTAA AGACTGTGCA GAACAGGGAG CACTTTGAGC CCACCCAGTA CAGCATGGAC 
CACTTCTCAG GGATGCACAA TTGGTACGCC TGTTCCCACG CGAGGCCGAC CTACTGCAAT 
GTGTGCCGTG AGGCTCTGTC TGGGGTCACG TCGCACGGGC TGTCCTGCGA GGTGTGCAAA 
TTTAAGGCCC ACAAGCGCTG TGCTGTGCGT GCAACCAATA ACTGCAAGTG GACCACACTG 
GCCTCGATCG GGAAGGACAT CATTGAAGAT GCAGATGGGA TTGCAATGCC CCACCAGTGG 
TTGGAAGGAA ACCTACCTGT GAGCGCCAAG TGCACTGTGT GCGACAAGAC CTGTGGCAGT 
GTGCTGCGCC TGCAGGACTG GCGCTGCCTC TGGTGCAAGG CCATGGTTCA CACATCGTGT 
AAAGAATCCT TGCTGACCAA GTGCCCACTT GGCCTGTGCA AAGTGTCAGT CATCCCACCC 
ACGGCTCTCA ACAGCATCGA CTCCGATGGG TTCTGGAAGG CCAGCTGTCC TCCTTCTTGC 
ACAAGCCCAC TGTTGGTCTT CGTCAATTCA AAAAGTGGGG ACAACCAGGG TGTGAAGTTC 
CTCAGAAGAT TCAAACAGCT ACTAAACCCC GCCCAGGTCT TCGACCTCAT GAACGGAGGC 
CCACACCTCG GCTTACGGTT ATTCCAGAAG TTTGACACAT TCCGGATTCT GGTTTGTGGC 
GGGGATGGAA GTGTTGGCTG GGTCCTCTCC GAAATCGACA GCCTCAACCT TCATAAACAG 
TGTCAGCTGG GAGTGCTGCC GCTCGGCACA GGGAACGACT TGGCCCGAGT ACTGGGCTGG 
GGCTCAGCCT GCGATGACGA CACCCAGCTC CCCCAGATCT TGGAGAAGTT GGAGAGAGCC 
AGCACCAAGA TGCTGGACAG GTGGAGCGTC ATGGCATACG AGGCCAAGCT CCCCCGGCAG 
GCCTCCTCCT CTACCGTCAC CGAAGACTTC AGCGAGGATT CCGAGGTACA GCAGATTCTC 
TTCTATGAAG ACTCGGTTGC AGCCCACCTT TCTAAAATCC TCACCTCGGA CCAGCACTCG 
GTGGTCATCT CCTCGGCCAA AGTGCTCTGT GAGACGGTGA AGGACTTCGT GGCACGGGTG 
GGGAAGGCCT ATGAGAAGAC GACCGAGAGC TCGGAGGAGT CAGAGGTCAT GGCCAAGAAG 
TGCTCTGTCC TGAAAGAGAA GCTGGATTCC CTTCTCAAGA CCTTGGACGA TGAGTCCCAG 
GCCTCGTCCT CTCTGCCCAA CCCGCCCCCC ACCATTGCCG AGGAGGCTGA AGATGGAGAT 
GGGTCGGGCA GCATCTGCGG TTCCACCGGA GACCGCTTGG TGGCATCAGC TTGCCCGGCC 
CGGCCGCAGA TATTCCGGCC TCGAGAACAG CTCATGCTGA GAGCCAACAG CCTGAAGAAA 
GCAATTCGTC AGATCATAGA ACACACAGAA AAAGCTGTCG ATGAGCAGAA TGCCCAGACC 
CAGGAGCAGG AGGGCTTCGT CCTGGGCCTC TCTGAGTCAG AGGAGAAGAT GGACCACAGA 
GTGTGCCCAC CACTGTCCCA CAGCGAGAGC TTCGGGGTCC CCAAGGGGAG GAGCCAGCGC 
AAAGTGTCGA AATCTCCGTG TGAAAAGCTG ATCAGCAAAG GGAGTCTGTC CCTAGGCAGT 
TCTGCTTCCC TTCCGCCCCA GCCGGGAAGC CGGGACGGCC TGCCTGCGCT CAACACCAAG 
ATCCTGTACC CAAATGTCCG GGCTGGAATG TCTGGTTCCT TACCCGGTGG CTCAGTCATC 
AGTCGCCTGT TAATTAATGC TGATCCCTTC AACTCTGAAC CAGAAACCCT AGAGTATTAC 
ACGGAGAAAT GTGTCATGAA CAACTATTTT GGCATTGGCC TGGATGCGAA GATATCCCTG 
GACTTTAACA ACAAGCGCGA TGAGCACCCA GAGAAGTGCA GGAGCCGAAC CAAGAACATG 
ATGTGGTATG GAGTTCTTGG AACCAAAGAG TTGCTGCACA GAACCTACAA GAACCTGGAG 
CAAAAGGTCT TGCTGGAGTG TGACGGGCGA CCCATCCCAC TCCCCAGTCT TCAGGGAATT 
GCTGTCCTTA ACATTCCCAG CTATGCCGGA GGAACCAACT TCTGGGGGGG TACCAAGGAA 
GATGATACTT TCGCAGCTCC ATCATTCGAT GACAAGATTC TGGAGGTGGT CGCCGTGTTC 
GGCAGCATGC AGATGGCCGT CTCTCGAGTC ATCAGGCTAC AGCATCATCG GATCGCCCAG 
TGTCGCACGG TGAAGATCTC CATCCTTGGG GATGAGGGCG TGCCTGTGCA GGTGGACGGA 
GAGGCCTGGG TCCAGCCGCC AGGGTACATT CGGATTGTCC ACAAGAACCG GGCACAGACA 
CTGACCAGAG ACAGGGCATT TGAGAGCACC CTGAAGTCCT GGGAAGACAA GCAGAAGTGC 
GAGCTGCCCC GCCCTCCATC CTGTTCCCTG CACCCGGAGA TGCTGTCCGA GGAGGAGGCC 
ACCCAGATGG ACCAGTTTGG GCAGGCAGCA GGGGTCCTCA TTCACAGTAT CCGAGAAATA 
GCTCAGTCTC ACCGGGACAT GGAGCAGGAA CTGGCCCACG CCGTCAATGC CAGCTCCAAG 
TCCATGGACC GTGTGTATGG CAAGCCCAGA ACCACAGAGG GGCTCAACTG CAGCTTCGTC 
CTGGAAATGG TGAATAACTT CAGAGCTCTG CGCAGTGAGA CGGAGCTGCT GCTGTCTGGG 
AAGATGGCCC TGCAGCTGGA TCCGCCTCAG AAGGAGCAGC TGGGGAGTGC TCTTGCCGAG 
ATGGACCGAC AGCTCAGGAG GCTGGCAGAC ACCCCGTGGC TCTGCCAGTC CGCAGAGCCC 
GGCGACGAAG AGAGTGTGAT GCTGGATCTT GCCAAGCGCA GTCGCAGTGG TAAATTCCGC 
CTCGTGACCA AGTTTAAAAA GGAGAAAAAC AACAAGAACA AAGAAGCTCA CAGTAGCCTG 
GGAGCCCCGG TTCACCTCTG GGGGACAGAG GAGGTTGCTG CCTGGCTGGA GCACCTCAGT 
CTCTGTGAGT ATAAGGACAT CTTCACACGG CACGACATCC GGGGCTCTGA GCTCCTGCAC 
CTGGAGCGGA GGGACCTCAA GGACCTGGGC GTGACCAAGG TGGGCCACAT GAAGAGGATC 
CTGTGTGGCA TCAAGGAGCT GAGCCGCAGC GCCCCCGCCG TCGAGGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512033.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510335.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X7, mRNA.

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CloneID	OHu61927
Clone ID Related Accession (Same CDS sequence)	XM_011512033.2 , XM_011512033.3
Accession Version	XM_011512033.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512033.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTTC AGACTCCAGG GACAGCTGTC ACCCACCAAA AACTGGGGTC ACAAGGGGTC 
CATCTGGCAG TGGCTGGAGC CTTGCAAGGG CACAGCTGCT GCTGCGGATG TACTGTTCCC 
CTCCTGCCTG GGAACCCTGG CCTTGCTGGG GTCACCTCCT CCACAACTTG GACTGGAGCA 
GAAGGACAAA GAATGGATCT GAGGACAAGC TGGCCCAGGA CCAGAACTAC CATCATCAAA 
GAGGGGATGC TGACCAAACA GAACAATTCA TTCCAGCGAT CAAAAAGGAG ATACTTTAAG 
CTTCGAGGGC GAACGCTTTA CTATGCCAAA ACGGCAAAGT CAATCATATT TGATGAGGTG 
GATCTGACAG ATGCCAGCGT AGCTGAATCC AGTACCAAAA ACGTCAACAA CAGTTTTACG 
GTCATAACTC CATGCAGGAA GCTCATCTTG TGTGCTGATA ACAGAAAAGA AATGGAAGAT 
TGGATTGCAG CATTAAAGAC TGTGCAGAAC AGGGAGCACT TTGAGCCCAC CCAGTACAGC 
ATGGACCACT TCTCAGGGAT GCACAATTGG TACGCCTGTT CCCACGCGAG GCCGACCTAC 
TGCAATGTGT GCCGTGAGGC TCTGTCTGGG GTCACGTCGC ACGGGCTGTC CTGCGAGGTG 
TGCAAATTTA AGGCCCACAA GCGCTGTGCT GTGCGTGCAA CCAATAACTG CAAGTGGACC 
ACACTGGCCT CGATCGGGAA GGACATCATT GAAGATGCAG ATGGGATTGC AATGCCCCAC 
CAGTGGTTGG AAGGAAACCT ACCTGTGAGC GCCAAGTGCA CTGTGTGCGA CAAGACCTGT 
GGCAGTGTGC TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCGTGTAAAG AATCCTTGCT GACCAAGTGC CCACTTGGCC TGTGCAAAGT GTCAGTCATC 
CCACCCACGG CTCTCAACAG CATCGACTCC GATGGGTTCT GGAAGGCCAG CTGTCCTCCT 
TCTTGCACAA GCCCACTGTT GGTCTTCGTC AATTCAAAAA GTGGGGACAA CCAGGGTGTG 
AAGTTCCTCA GAAGATTCAA ACAGCTACTA AACCCCGCCC AGGTCTTCGA CCTCATGAAC 
GGAGGCCCAC ACCTCGGCTT ACGGTTATTC CAGAAGTTTG ACACATTCCG GATTCTGGTT 
TGTGGCGGGG ATGGAAGTGT TGGCTGGGTC CTCTCCGAAA TCGACAGCCT CAACCTTCAT 
AAACAGTGTC AGCTGGGAGT GCTGCCGCTC GGCACAGGGA ACGACTTGGC CCGAGTACTG 
GGCTGGGGCT CAGCCTGCGA TGACGACACC CAGCTCCCCC AGATCTTGGA GAAGTTGGAG 
AGAGCCAGCA CCAAGATGCT GGACAGGTGG AGCGTCATGG CATACGAGGC CAAGCTCCCC 
CGGCAGGCCT CCTCCTCTAC CGTCACCGAA GACTTCAGCG AGGATTCCGA GGTACAGCAG 
ATTCTCTTCT ATGAAGACTC GGTTGCAGCC CACCTTTCTA AAATCCTCAC CTCGGACCAG 
CACTCGGTGG TCATCTCCTC GGCCAAAGTG CTCTGTGAGA CGGTGAAGGA CTTCGTGGCA 
CGGGTGGGGA AGGCCTATGA GAAGACGACC GAGAGCTCGG AGGAGTCAGA GGTCATGGCC 
AAGAAGTGCT CTGTCCTGAA AGAGAAGCTG GATTCCCTTC TCAAGACCTT GGACGATGAG 
TCCCAGGCCT CGTCCTCTCT GCCCAACCCG CCCCCCACCA TTGCCGAGGA GGCTGAAGAT 
GGAGATGGGT CGGGCAGCAT CTGCGGTTCC ACCGGAGACC GCTTGGTGGC ATCAGCTTGC 
CCGGCCCGGC CGCAGATATT CCGGCCTCGA GAACAGCTCA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGTCAGAT CATAGAACAC ACAGAAAAAG CTGTCGATGA GCAGAATGCC 
CAGACCCAGG AGCAGGAGGG CTTCGTCCTG GGCCTCTCTG AGTCAGAGGA GAAGATGGAC 
CACAGAGTGT GCCCACCACT GTCCCACAGC GAGAGCTTCG GGGTCCCCAA GGGGAGGAGC 
CAGCGCAAAG TGTCGAAATC TCCGTGTGAA AAGCTGATCA GCAAAGGGAG TCTGTCCCTA 
GGCAGTTCTG CTTCCCTTCC GCCCCAGCCG GGAAGCCGGG ACGGCCTGCC TGCGCTCAAC 
ACCAAGATCC TGTACCCAAA TGTCCGGGCT GGAATGTCTG GTTCCTTACC CGGTGGCTCA 
GTCATCAGTC GCCTGTTAAT TAATGCTGAT CCCTTCAACT CTGAACCAGA AACCCTAGAG 
TATTACACGG AGAAATGTGT CATGAACAAC TATTTTGGCA TTGGCCTGGA TGCGAAGATA 
TCCCTGGACT TTAACAACAA GCGCGATGAG CACCCAGAGA AGTGCAGGAG CCGAACCAAG 
AACATGATGT GGTATGGAGT TCTTGGAACC AAAGAGTTGC TGCACAGAAC CTACAAGAAC 
CTGGAGCAAA AGGTCTTGCT GGAGTGTGAC GGGCGACCCA TCCCACTCCC CAGTCTTCAG 
GGAATTGCTG TCCTTAACAT TCCCAGCTAT GCCGGAGGAA CCAACTTCTG GGGGGGTACC 
AAGGAAGATG ATACTTTCGC AGCTCCATCA TTCGATGACA AGATTCTGGA GGTGGTCGCC 
GTGTTCGGCA GCATGCAGAT GGCCGTCTCT CGAGTCATCA GGCTACAGCA TCATCGGATC 
GCCCAGTGTC GCACGGTGAA GATCTCCATC CTTGGGGATG AGGGCGTGCC TGTGCAGGTG 
GACGGAGAGG CCTGGGTCCA GCCGCCAGGG TACATTCGGA TTGTCCACAA GAACCGGGCA 
CAGACACTGA CCAGAGACAG GGCATTTGAG AGCACCCTGA AGTCCTGGGA AGACAAGCAG 
AAGTGCGAGC TGCCCCGCCC TCCATCCTGT TCCCTGCACC CGGAGATGCT GTCCGAGGAG 
GAGGCCACCC AGATGGACCA GTTTGGGCAG GCAGCAGGGG TCCTCATTCA CAGTATCCGA 
GAAATAGCTC AGTCTCACCG GGACATGGAG CAGGAACTGG CCCACGCCGT CAATGCCAGC 
TCCAAGTCCA TGGACCGTGT GTATGGCAAG CCCAGAACCA CAGAGGGGCT CAACTGCAGC 
TTCGTCCTGG AAATGGTGAA TAACTTCAGA GCTCTGCGCA GTGAGACGGA GCTGCTGCTG 
TCTGGGAAGA TGGCCCTGCA TTTCCACTGC TTGACACAGG TGAATGGCTC CGACCCCATT 
TCTGACAGAG GGACACAAGC AGGTCAGTTG TCAGCCTTGC TCTATGTGAA TTCATCGTTG 
ACTGATGGAC AGTTTTATTC TCTCACCAGC ACTACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510335.1
CDS	1402..4800
Translation	MAFQTPGTAVTHQKLGSQGVHLAVAGALQGHSCCCGCTVPLLPGNPGLAGVTSSTTWTGAEGQRMDLRTSWPRTRTTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALHFHCLTQVNGSDPISDRGTQAGQLSALLYVNSSLTDGQFYSLTSTT

Target ORF information:

RefSeq Version	XM_011512033.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512033.2

ORF Insert Sequence:

ATGGCATTTC AGACTCCAGG GACAGCTGTC ACCCACCAAA AACTGGGGTC ACAAGGGGTC 
CATCTGGCAG TGGCTGGAGC CTTGCAAGGG CACAGCTGCT GCTGCGGATG TACTGTTCCC 
CTCCTGCCTG GGAACCCTGG CCTTGCTGGG GTCACCTCCT CCACAACTTG GACTGGAGCA 
GAAGGACAAA GAATGGATCT GAGGACAAGC TGGCCCAGGA CCAGAACTAC CATCATCAAA 
GAGGGGATGC TGACCAAACA GAACAATTCA TTCCAGCGAT CAAAAAGGAG ATACTTTAAG 
CTTCGAGGGC GAACGCTTTA CTATGCCAAA ACGGCAAAGT CAATCATATT TGATGAGGTG 
GATCTGACAG ATGCCAGCGT AGCTGAATCC AGTACCAAAA ACGTCAACAA CAGTTTTACG 
GTCATAACTC CATGCAGGAA GCTCATCTTG TGTGCTGATA ACAGAAAAGA AATGGAAGAT 
TGGATTGCAG CATTAAAGAC TGTGCAGAAC AGGGAGCACT TTGAGCCCAC CCAGTACAGC 
ATGGACCACT TCTCAGGGAT GCACAATTGG TACGCCTGTT CCCACGCGAG GCCGACCTAC 
TGCAATGTGT GCCGTGAGGC TCTGTCTGGG GTCACGTCGC ACGGGCTGTC CTGCGAGGTG 
TGCAAATTTA AGGCCCACAA GCGCTGTGCT GTGCGTGCAA CCAATAACTG CAAGTGGACC 
ACACTGGCCT CGATCGGGAA GGACATCATT GAAGATGCAG ATGGGATTGC AATGCCCCAC 
CAGTGGTTGG AAGGAAACCT ACCTGTGAGC GCCAAGTGCA CTGTGTGCGA CAAGACCTGT 
GGCAGTGTGC TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCGTGTAAAG AATCCTTGCT GACCAAGTGC CCACTTGGCC TGTGCAAAGT GTCAGTCATC 
CCACCCACGG CTCTCAACAG CATCGACTCC GATGGGTTCT GGAAGGCCAG CTGTCCTCCT 
TCTTGCACAA GCCCACTGTT GGTCTTCGTC AATTCAAAAA GTGGGGACAA CCAGGGTGTG 
AAGTTCCTCA GAAGATTCAA ACAGCTACTA AACCCCGCCC AGGTCTTCGA CCTCATGAAC 
GGAGGCCCAC ACCTCGGCTT ACGGTTATTC CAGAAGTTTG ACACATTCCG GATTCTGGTT 
TGTGGCGGGG ATGGAAGTGT TGGCTGGGTC CTCTCCGAAA TCGACAGCCT CAACCTTCAT 
AAACAGTGTC AGCTGGGAGT GCTGCCGCTC GGCACAGGGA ACGACTTGGC CCGAGTACTG 
GGCTGGGGCT CAGCCTGCGA TGACGACACC CAGCTCCCCC AGATCTTGGA GAAGTTGGAG 
AGAGCCAGCA CCAAGATGCT GGACAGGTGG AGCGTCATGG CATACGAGGC CAAGCTCCCC 
CGGCAGGCCT CCTCCTCTAC CGTCACCGAA GACTTCAGCG AGGATTCCGA GGTACAGCAG 
ATTCTCTTCT ATGAAGACTC GGTTGCAGCC CACCTTTCTA AAATCCTCAC CTCGGACCAG 
CACTCGGTGG TCATCTCCTC GGCCAAAGTG CTCTGTGAGA CGGTGAAGGA CTTCGTGGCA 
CGGGTGGGGA AGGCCTATGA GAAGACGACC GAGAGCTCGG AGGAGTCAGA GGTCATGGCC 
AAGAAGTGCT CTGTCCTGAA AGAGAAGCTG GATTCCCTTC TCAAGACCTT GGACGATGAG 
TCCCAGGCCT CGTCCTCTCT GCCCAACCCG CCCCCCACCA TTGCCGAGGA GGCTGAAGAT 
GGAGATGGGT CGGGCAGCAT CTGCGGTTCC ACCGGAGACC GCTTGGTGGC ATCAGCTTGC 
CCGGCCCGGC CGCAGATATT CCGGCCTCGA GAACAGCTCA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGTCAGAT CATAGAACAC ACAGAAAAAG CTGTCGATGA GCAGAATGCC 
CAGACCCAGG AGCAGGAGGG CTTCGTCCTG GGCCTCTCTG AGTCAGAGGA GAAGATGGAC 
CACAGAGTGT GCCCACCACT GTCCCACAGC GAGAGCTTCG GGGTCCCCAA GGGGAGGAGC 
CAGCGCAAAG TGTCGAAATC TCCGTGTGAA AAGCTGATCA GCAAAGGGAG TCTGTCCCTA 
GGCAGTTCTG CTTCCCTTCC GCCCCAGCCG GGAAGCCGGG ACGGCCTGCC TGCGCTCAAC 
ACCAAGATCC TGTACCCAAA TGTCCGGGCT GGAATGTCTG GTTCCTTACC CGGTGGCTCA 
GTCATCAGTC GCCTGTTAAT TAATGCTGAT CCCTTCAACT CTGAACCAGA AACCCTAGAG 
TATTACACGG AGAAATGTGT CATGAACAAC TATTTTGGCA TTGGCCTGGA TGCGAAGATA 
TCCCTGGACT TTAACAACAA GCGCGATGAG CACCCAGAGA AGTGCAGGAG CCGAACCAAG 
AACATGATGT GGTATGGAGT TCTTGGAACC AAAGAGTTGC TGCACAGAAC CTACAAGAAC 
CTGGAGCAAA AGGTCTTGCT GGAGTGTGAC GGGCGACCCA TCCCACTCCC CAGTCTTCAG 
GGAATTGCTG TCCTTAACAT TCCCAGCTAT GCCGGAGGAA CCAACTTCTG GGGGGGTACC 
AAGGAAGATG ATACTTTCGC AGCTCCATCA TTCGATGACA AGATTCTGGA GGTGGTCGCC 
GTGTTCGGCA GCATGCAGAT GGCCGTCTCT CGAGTCATCA GGCTACAGCA TCATCGGATC 
GCCCAGTGTC GCACGGTGAA GATCTCCATC CTTGGGGATG AGGGCGTGCC TGTGCAGGTG 
GACGGAGAGG CCTGGGTCCA GCCGCCAGGG TACATTCGGA TTGTCCACAA GAACCGGGCA 
CAGACACTGA CCAGAGACAG GGCATTTGAG AGCACCCTGA AGTCCTGGGA AGACAAGCAG 
AAGTGCGAGC TGCCCCGCCC TCCATCCTGT TCCCTGCACC CGGAGATGCT GTCCGAGGAG 
GAGGCCACCC AGATGGACCA GTTTGGGCAG GCAGCAGGGG TCCTCATTCA CAGTATCCGA 
GAAATAGCTC AGTCTCACCG GGACATGGAG CAGGAACTGG CCCACGCCGT CAATGCCAGC 
TCCAAGTCCA TGGACCGTGT GTATGGCAAG CCCAGAACCA CAGAGGGGCT CAACTGCAGC 
TTCGTCCTGG AAATGGTGAA TAACTTCAGA GCTCTGCGCA GTGAGACGGA GCTGCTGCTG 
TCTGGGAAGA TGGCCCTGCA TTTCCACTGC TTGACACAGG TGAATGGCTC CGACCCCATT 
TCTGACAGAG GGACACAAGC AGGTCAGTTG TCAGCCTTGC TCTATGTGAA TTCATCGTTG 
ACTGATGGAC AGTTTTATTC TCTCACCAGC ACTACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011512033.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510335.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X8, mRNA.

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CloneID	OHu61927
Clone ID Related Accession (Same CDS sequence)	XM_011512033.2 , XM_011512033.3
Accession Version	XM_011512033.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512033.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTTC AGACTCCAGG GACAGCTGTC ACCCACCAAA AACTGGGGTC ACAAGGGGTC 
CATCTGGCAG TGGCTGGAGC CTTGCAAGGG CACAGCTGCT GCTGCGGATG TACTGTTCCC 
CTCCTGCCTG GGAACCCTGG CCTTGCTGGG GTCACCTCCT CCACAACTTG GACTGGAGCA 
GAAGGACAAA GAATGGATCT GAGGACAAGC TGGCCCAGGA CCAGAACTAC CATCATCAAA 
GAGGGGATGC TGACCAAACA GAACAATTCA TTCCAGCGAT CAAAAAGGAG ATACTTTAAG 
CTTCGAGGGC GAACGCTTTA CTATGCCAAA ACGGCAAAGT CAATCATATT TGATGAGGTG 
GATCTGACAG ATGCCAGCGT AGCTGAATCC AGTACCAAAA ACGTCAACAA CAGTTTTACG 
GTCATAACTC CATGCAGGAA GCTCATCTTG TGTGCTGATA ACAGAAAAGA AATGGAAGAT 
TGGATTGCAG CATTAAAGAC TGTGCAGAAC AGGGAGCACT TTGAGCCCAC CCAGTACAGC 
ATGGACCACT TCTCAGGGAT GCACAATTGG TACGCCTGTT CCCACGCGAG GCCGACCTAC 
TGCAATGTGT GCCGTGAGGC TCTGTCTGGG GTCACGTCGC ACGGGCTGTC CTGCGAGGTG 
TGCAAATTTA AGGCCCACAA GCGCTGTGCT GTGCGTGCAA CCAATAACTG CAAGTGGACC 
ACACTGGCCT CGATCGGGAA GGACATCATT GAAGATGCAG ATGGGATTGC AATGCCCCAC 
CAGTGGTTGG AAGGAAACCT ACCTGTGAGC GCCAAGTGCA CTGTGTGCGA CAAGACCTGT 
GGCAGTGTGC TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCGTGTAAAG AATCCTTGCT GACCAAGTGC CCACTTGGCC TGTGCAAAGT GTCAGTCATC 
CCACCCACGG CTCTCAACAG CATCGACTCC GATGGGTTCT GGAAGGCCAG CTGTCCTCCT 
TCTTGCACAA GCCCACTGTT GGTCTTCGTC AATTCAAAAA GTGGGGACAA CCAGGGTGTG 
AAGTTCCTCA GAAGATTCAA ACAGCTACTA AACCCCGCCC AGGTCTTCGA CCTCATGAAC 
GGAGGCCCAC ACCTCGGCTT ACGGTTATTC CAGAAGTTTG ACACATTCCG GATTCTGGTT 
TGTGGCGGGG ATGGAAGTGT TGGCTGGGTC CTCTCCGAAA TCGACAGCCT CAACCTTCAT 
AAACAGTGTC AGCTGGGAGT GCTGCCGCTC GGCACAGGGA ACGACTTGGC CCGAGTACTG 
GGCTGGGGCT CAGCCTGCGA TGACGACACC CAGCTCCCCC AGATCTTGGA GAAGTTGGAG 
AGAGCCAGCA CCAAGATGCT GGACAGGTGG AGCGTCATGG CATACGAGGC CAAGCTCCCC 
CGGCAGGCCT CCTCCTCTAC CGTCACCGAA GACTTCAGCG AGGATTCCGA GGTACAGCAG 
ATTCTCTTCT ATGAAGACTC GGTTGCAGCC CACCTTTCTA AAATCCTCAC CTCGGACCAG 
CACTCGGTGG TCATCTCCTC GGCCAAAGTG CTCTGTGAGA CGGTGAAGGA CTTCGTGGCA 
CGGGTGGGGA AGGCCTATGA GAAGACGACC GAGAGCTCGG AGGAGTCAGA GGTCATGGCC 
AAGAAGTGCT CTGTCCTGAA AGAGAAGCTG GATTCCCTTC TCAAGACCTT GGACGATGAG 
TCCCAGGCCT CGTCCTCTCT GCCCAACCCG CCCCCCACCA TTGCCGAGGA GGCTGAAGAT 
GGAGATGGGT CGGGCAGCAT CTGCGGTTCC ACCGGAGACC GCTTGGTGGC ATCAGCTTGC 
CCGGCCCGGC CGCAGATATT CCGGCCTCGA GAACAGCTCA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGTCAGAT CATAGAACAC ACAGAAAAAG CTGTCGATGA GCAGAATGCC 
CAGACCCAGG AGCAGGAGGG CTTCGTCCTG GGCCTCTCTG AGTCAGAGGA GAAGATGGAC 
CACAGAGTGT GCCCACCACT GTCCCACAGC GAGAGCTTCG GGGTCCCCAA GGGGAGGAGC 
CAGCGCAAAG TGTCGAAATC TCCGTGTGAA AAGCTGATCA GCAAAGGGAG TCTGTCCCTA 
GGCAGTTCTG CTTCCCTTCC GCCCCAGCCG GGAAGCCGGG ACGGCCTGCC TGCGCTCAAC 
ACCAAGATCC TGTACCCAAA TGTCCGGGCT GGAATGTCTG GTTCCTTACC CGGTGGCTCA 
GTCATCAGTC GCCTGTTAAT TAATGCTGAT CCCTTCAACT CTGAACCAGA AACCCTAGAG 
TATTACACGG AGAAATGTGT CATGAACAAC TATTTTGGCA TTGGCCTGGA TGCGAAGATA 
TCCCTGGACT TTAACAACAA GCGCGATGAG CACCCAGAGA AGTGCAGGAG CCGAACCAAG 
AACATGATGT GGTATGGAGT TCTTGGAACC AAAGAGTTGC TGCACAGAAC CTACAAGAAC 
CTGGAGCAAA AGGTCTTGCT GGAGTGTGAC GGGCGACCCA TCCCACTCCC CAGTCTTCAG 
GGAATTGCTG TCCTTAACAT TCCCAGCTAT GCCGGAGGAA CCAACTTCTG GGGGGGTACC 
AAGGAAGATG ATACTTTCGC AGCTCCATCA TTCGATGACA AGATTCTGGA GGTGGTCGCC 
GTGTTCGGCA GCATGCAGAT GGCCGTCTCT CGAGTCATCA GGCTACAGCA TCATCGGATC 
GCCCAGTGTC GCACGGTGAA GATCTCCATC CTTGGGGATG AGGGCGTGCC TGTGCAGGTG 
GACGGAGAGG CCTGGGTCCA GCCGCCAGGG TACATTCGGA TTGTCCACAA GAACCGGGCA 
CAGACACTGA CCAGAGACAG GGCATTTGAG AGCACCCTGA AGTCCTGGGA AGACAAGCAG 
AAGTGCGAGC TGCCCCGCCC TCCATCCTGT TCCCTGCACC CGGAGATGCT GTCCGAGGAG 
GAGGCCACCC AGATGGACCA GTTTGGGCAG GCAGCAGGGG TCCTCATTCA CAGTATCCGA 
GAAATAGCTC AGTCTCACCG GGACATGGAG CAGGAACTGG CCCACGCCGT CAATGCCAGC 
TCCAAGTCCA TGGACCGTGT GTATGGCAAG CCCAGAACCA CAGAGGGGCT CAACTGCAGC 
TTCGTCCTGG AAATGGTGAA TAACTTCAGA GCTCTGCGCA GTGAGACGGA GCTGCTGCTG 
TCTGGGAAGA TGGCCCTGCA TTTCCACTGC TTGACACAGG TGAATGGCTC CGACCCCATT 
TCTGACAGAG GGACACAAGC AGGTCAGTTG TCAGCCTTGC TCTATGTGAA TTCATCGTTG 
ACTGATGGAC AGTTTTATTC TCTCACCAGC ACTACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510335.1
CDS	1403..4801
Translation	MAFQTPGTAVTHQKLGSQGVHLAVAGALQGHSCCCGCTVPLLPGNPGLAGVTSSTTWTGAEGQRMDLRTSWPRTRTTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALHFHCLTQVNGSDPISDRGTQAGQLSALLYVNSSLTDGQFYSLTSTT

Target ORF information:

RefSeq Version	XM_011512033.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512033.3

ORF Insert Sequence:

ATGGCATTTC AGACTCCAGG GACAGCTGTC ACCCACCAAA AACTGGGGTC ACAAGGGGTC 
CATCTGGCAG TGGCTGGAGC CTTGCAAGGG CACAGCTGCT GCTGCGGATG TACTGTTCCC 
CTCCTGCCTG GGAACCCTGG CCTTGCTGGG GTCACCTCCT CCACAACTTG GACTGGAGCA 
GAAGGACAAA GAATGGATCT GAGGACAAGC TGGCCCAGGA CCAGAACTAC CATCATCAAA 
GAGGGGATGC TGACCAAACA GAACAATTCA TTCCAGCGAT CAAAAAGGAG ATACTTTAAG 
CTTCGAGGGC GAACGCTTTA CTATGCCAAA ACGGCAAAGT CAATCATATT TGATGAGGTG 
GATCTGACAG ATGCCAGCGT AGCTGAATCC AGTACCAAAA ACGTCAACAA CAGTTTTACG 
GTCATAACTC CATGCAGGAA GCTCATCTTG TGTGCTGATA ACAGAAAAGA AATGGAAGAT 
TGGATTGCAG CATTAAAGAC TGTGCAGAAC AGGGAGCACT TTGAGCCCAC CCAGTACAGC 
ATGGACCACT TCTCAGGGAT GCACAATTGG TACGCCTGTT CCCACGCGAG GCCGACCTAC 
TGCAATGTGT GCCGTGAGGC TCTGTCTGGG GTCACGTCGC ACGGGCTGTC CTGCGAGGTG 
TGCAAATTTA AGGCCCACAA GCGCTGTGCT GTGCGTGCAA CCAATAACTG CAAGTGGACC 
ACACTGGCCT CGATCGGGAA GGACATCATT GAAGATGCAG ATGGGATTGC AATGCCCCAC 
CAGTGGTTGG AAGGAAACCT ACCTGTGAGC GCCAAGTGCA CTGTGTGCGA CAAGACCTGT 
GGCAGTGTGC TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCGTGTAAAG AATCCTTGCT GACCAAGTGC CCACTTGGCC TGTGCAAAGT GTCAGTCATC 
CCACCCACGG CTCTCAACAG CATCGACTCC GATGGGTTCT GGAAGGCCAG CTGTCCTCCT 
TCTTGCACAA GCCCACTGTT GGTCTTCGTC AATTCAAAAA GTGGGGACAA CCAGGGTGTG 
AAGTTCCTCA GAAGATTCAA ACAGCTACTA AACCCCGCCC AGGTCTTCGA CCTCATGAAC 
GGAGGCCCAC ACCTCGGCTT ACGGTTATTC CAGAAGTTTG ACACATTCCG GATTCTGGTT 
TGTGGCGGGG ATGGAAGTGT TGGCTGGGTC CTCTCCGAAA TCGACAGCCT CAACCTTCAT 
AAACAGTGTC AGCTGGGAGT GCTGCCGCTC GGCACAGGGA ACGACTTGGC CCGAGTACTG 
GGCTGGGGCT CAGCCTGCGA TGACGACACC CAGCTCCCCC AGATCTTGGA GAAGTTGGAG 
AGAGCCAGCA CCAAGATGCT GGACAGGTGG AGCGTCATGG CATACGAGGC CAAGCTCCCC 
CGGCAGGCCT CCTCCTCTAC CGTCACCGAA GACTTCAGCG AGGATTCCGA GGTACAGCAG 
ATTCTCTTCT ATGAAGACTC GGTTGCAGCC CACCTTTCTA AAATCCTCAC CTCGGACCAG 
CACTCGGTGG TCATCTCCTC GGCCAAAGTG CTCTGTGAGA CGGTGAAGGA CTTCGTGGCA 
CGGGTGGGGA AGGCCTATGA GAAGACGACC GAGAGCTCGG AGGAGTCAGA GGTCATGGCC 
AAGAAGTGCT CTGTCCTGAA AGAGAAGCTG GATTCCCTTC TCAAGACCTT GGACGATGAG 
TCCCAGGCCT CGTCCTCTCT GCCCAACCCG CCCCCCACCA TTGCCGAGGA GGCTGAAGAT 
GGAGATGGGT CGGGCAGCAT CTGCGGTTCC ACCGGAGACC GCTTGGTGGC ATCAGCTTGC 
CCGGCCCGGC CGCAGATATT CCGGCCTCGA GAACAGCTCA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGTCAGAT CATAGAACAC ACAGAAAAAG CTGTCGATGA GCAGAATGCC 
CAGACCCAGG AGCAGGAGGG CTTCGTCCTG GGCCTCTCTG AGTCAGAGGA GAAGATGGAC 
CACAGAGTGT GCCCACCACT GTCCCACAGC GAGAGCTTCG GGGTCCCCAA GGGGAGGAGC 
CAGCGCAAAG TGTCGAAATC TCCGTGTGAA AAGCTGATCA GCAAAGGGAG TCTGTCCCTA 
GGCAGTTCTG CTTCCCTTCC GCCCCAGCCG GGAAGCCGGG ACGGCCTGCC TGCGCTCAAC 
ACCAAGATCC TGTACCCAAA TGTCCGGGCT GGAATGTCTG GTTCCTTACC CGGTGGCTCA 
GTCATCAGTC GCCTGTTAAT TAATGCTGAT CCCTTCAACT CTGAACCAGA AACCCTAGAG 
TATTACACGG AGAAATGTGT CATGAACAAC TATTTTGGCA TTGGCCTGGA TGCGAAGATA 
TCCCTGGACT TTAACAACAA GCGCGATGAG CACCCAGAGA AGTGCAGGAG CCGAACCAAG 
AACATGATGT GGTATGGAGT TCTTGGAACC AAAGAGTTGC TGCACAGAAC CTACAAGAAC 
CTGGAGCAAA AGGTCTTGCT GGAGTGTGAC GGGCGACCCA TCCCACTCCC CAGTCTTCAG 
GGAATTGCTG TCCTTAACAT TCCCAGCTAT GCCGGAGGAA CCAACTTCTG GGGGGGTACC 
AAGGAAGATG ATACTTTCGC AGCTCCATCA TTCGATGACA AGATTCTGGA GGTGGTCGCC 
GTGTTCGGCA GCATGCAGAT GGCCGTCTCT CGAGTCATCA GGCTACAGCA TCATCGGATC 
GCCCAGTGTC GCACGGTGAA GATCTCCATC CTTGGGGATG AGGGCGTGCC TGTGCAGGTG 
GACGGAGAGG CCTGGGTCCA GCCGCCAGGG TACATTCGGA TTGTCCACAA GAACCGGGCA 
CAGACACTGA CCAGAGACAG GGCATTTGAG AGCACCCTGA AGTCCTGGGA AGACAAGCAG 
AAGTGCGAGC TGCCCCGCCC TCCATCCTGT TCCCTGCACC CGGAGATGCT GTCCGAGGAG 
GAGGCCACCC AGATGGACCA GTTTGGGCAG GCAGCAGGGG TCCTCATTCA CAGTATCCGA 
GAAATAGCTC AGTCTCACCG GGACATGGAG CAGGAACTGG CCCACGCCGT CAATGCCAGC 
TCCAAGTCCA TGGACCGTGT GTATGGCAAG CCCAGAACCA CAGAGGGGCT CAACTGCAGC 
TTCGTCCTGG AAATGGTGAA TAACTTCAGA GCTCTGCGCA GTGAGACGGA GCTGCTGCTG 
TCTGGGAAGA TGGCCCTGCA TTTCCACTGC TTGACACAGG TGAATGGCTC CGACCCCATT 
TCTGACAGAG GGACACAAGC AGGTCAGTTG TCAGCCTTGC TCTATGTGAA TTCATCGTTG 
ACTGATGGAC AGTTTTATTC TCTCACCAGC ACTACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_017005138.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860627.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X3, mRNA.

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CloneID	OHu61928
Clone ID Related Accession (Same CDS sequence)	XM_017005138.2 , XM_017005138.1
Accession Version	XM_017005138.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005138.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAC CTGAGGTCCT AACATGTTAC AAACAGACCA TCATCAAAGA GGGGATGCTG 
ACCAAACAGA ACAATTCATT CCAGCGATCA AAAAGGAGAT ACTTTAAGCT TCGAGGGCGA 
ACGCTTTACT ATGCCAAAAC GGCAAAGTCA ATCATATTTG ATGAGGTGGA TCTGACAGAT 
GCCAGCGTAG CTGAATCCAG TACCAAAAAC GTCAACAACA GTTTTACGGT CATAACTCCA 
TGCAGGAAGC TCATCTTGTG TGCTGATAAC AGAAAAGAAA TGGAAGATTG GATTGCAGCA 
TTAAAGACTG TGCAGAACAG GGAGCACTTT GAGCCCACCC AGTACAGCAT GGACCACTTC 
TCAGGGATGC ACAATTGGTA CGCCTGTTCC CACGCGAGGC CGACCTACTG CAATGTGTGC 
CGTGAGGCTC TGTCTGGGGT CACGTCGCAC GGGCTGTCCT GCGAGGTGTG CAAATTTAAG 
GCCCACAAGC GCTGTGCTGT GCGTGCAACC AATAACTGCA AGTGGACCAC ACTGGCCTCG 
ATCGGGAAGG ACATCATTGA AGATGCAGAT GGGATTGCAA TGCCCCACCA GTGGTTGGAA 
GGAAACCTAC CTGTGAGCGC CAAGTGCACT GTGTGCGACA AGACCTGTGG CAGTGTGCTG 
CGCCTGCAGG ACTGGCGCTG CCTCTGGTGC AAGGCCATGG TTCACACATC GTGTAAAGAA 
TCCTTGCTGA CCAAGTGCCC ACTTGGCCTG TGCAAAGTGT CAGTCATCCC ACCCACGGCT 
CTCAACAGCA TCGACTCCGA TGGGTTCTGG AAGGCCAGCT GTCCTCCTTC TTGCACAAGC 
CCACTGTTGG TCTTCGTCAA TTCAAAAAGT GGGGACAACC AGGGTGTGAA GTTCCTCAGA 
AGATTCAAAC AGCTACTAAA CCCCGCCCAG GTCTTCGACC TCATGAACGG AGGCCCACAC 
CTCGGCTTAC GGTTATTCCA GAAGTTTGAC ACATTCCGGA TTCTGGTTTG TGGCGGGGAT 
GGAAGTGTTG GCTGGGTCCT CTCCGAAATC GACAGCCTCA ACCTTCATAA ACAGTGTCAG 
CTGGGAGTGC TGCCGCTCGG CACAGGGAAC GACTTGGCCC GAGTACTGGG CTGGGGCTCA 
GCCTGCGATG ACGACACCCA GCTCCCCCAG ATCTTGGAGA AGTTGGAGAG AGCCAGCACC 
AAGATGCTGG ACAGGTGGAG CGTCATGGCA TACGAGGCCA AGCTCCCCCG GCAGGCCTCC 
TCCTCTACCG TCACCGAAGA CTTCAGCGAG GATTCCGAGG TACAGCAGAT TCTCTTCTAT 
GAAGACTCGG TTGCAGCCCA CCTTTCTAAA ATCCTCACCT CGGACCAGCA CTCGGTGGTC 
ATCTCCTCGG CCAAAGTGCT CTGTGAGACG GTGAAGGACT TCGTGGCACG GGTGGGGAAG 
GCCTATGAGA AGACGACCGA GAGCTCGGAG GAGTCAGAGG TCATGGCCAA GAAGTGCTCT 
GTCCTGAAAG AGAAGCTGGA TTCCCTTCTC AAGACCTTGG ACGATGAGTC CCAGGCCTCG 
TCCTCTCTGC CCAACCCGCC CCCCACCATT GCCGAGGAGG CTGAAGATGG AGATGGGTCG 
GGCAGCATCT GCGGTTCCAC CGGAGACCGC TTGGTGGCAT CAGCTTGCCC GGCCCGGCCG 
CAGATATTCC GGCCTCGAGA ACAGCTCATG CTGAGAGCCA ACAGCCTGAA GAAAGCAATT 
CGTCAGATCA TAGAACACAC AGAAAAAGCT GTCGATGAGC AGAATGCCCA GACCCAGGAG 
CAGGAGGGCT TCGTCCTGGG CCTCTCTGAG TCAGAGGAGA AGATGGACCA CAGAGTGTGC 
CCACCACTGT CCCACAGCGA GAGCTTCGGG GTCCCCAAGG GGAGGAGCCA GCGCAAAGTG 
TCGAAATCTC CGTGTGAAAA GCTGATCAGC AAAGGGAGTC TGTCCCTAGG CAGTTCTGCT 
TCCCTTCCGC CCCAGCCGGG AAGCCGGGAC GGCCTGCCTG CGCTCAACAC CAAGATCCTG 
TACCCAAATG TCCGGGCTGG AATGTCTGGT TCCTTACCCG GTGGCTCAGT CATCAGTCGC 
CTGTTAATTA ATGCTGATCC CTTCAACTCT GAACCAGAAA CCCTAGAGTA TTACACGGAG 
AAATGTGTCA TGAACAACTA TTTTGGCATT GGCCTGGATG CGAAGATATC CCTGGACTTT 
AACAACAAGC GCGATGAGCA CCCAGAGAAG TGCAGGAGCC GAACCAAGAA CATGATGTGG 
TATGGAGTTC TTGGAACCAA AGAGTTGCTG CACAGAACCT ACAAGAACCT GGAGCAAAAG 
GTCTTGCTGG AGTGTGACGG GCGACCCATC CCACTCCCCA GTCTTCAGGG AATTGCTGTC 
CTTAACATTC CCAGCTATGC CGGAGGAACC AACTTCTGGG GGGGTACCAA GGAAGATGAT 
ACTTTCGCAG CTCCATCATT CGATGACAAG ATTCTGGAGG TGGTCGCCGT GTTCGGCAGC 
ATGCAGATGG CCGTCTCTCG AGTCATCAGG CTACAGCATC ATCGGATCGC CCAGTGTCGC 
ACGGTGAAGA TCTCCATCCT TGGGGATGAG GGCGTGCCTG TGCAGGTGGA CGGAGAGGCC 
TGGGTCCAGC CGCCAGGGTA CATTCGGATT GTCCACAAGA ACCGGGCACA GACACTGACC 
AGAGACAGGG CATTTGAGAG CACCCTGAAG TCCTGGGAAG ACAAGCAGAA GTGCGAGCTG 
CCCCGCCCTC CATCCTGTTC CCTGCACCCG GAGATGCTGT CCGAGGAGGA GGCCACCCAG 
ATGGACCAGT TTGGGCAGGC AGCAGGGGTC CTCATTCACA GTATCCGAGA AATAGCTCAG 
TCTCACCGGG ACATGGAGCA GGAACTGGCC CACGCCGTCA ATGCCAGCTC CAAGTCCATG 
GACCGTGTGT ATGGCAAGCC CAGAACCACA GAGGGGCTCA ACTGCAGCTT CGTCCTGGAA 
ATGGTGAATA ACTTCAGAGC TCTGCGCAGT GAGACGGAGC TGCTGCTGTC TGGGAAGATG 
GCCCTGCAGC TGGATCCGCC TCAGAAGGAG CAGCTGGGGA GTGCTCTTGC CGAGATGGAC 
CGACAGCTCA GGAGGCTGGC AGACACCCCG TGGCTCTGCC AGTCCGCAGA GCCCGGCGAC 
GAAGAGAGTG TGATGCTGGA TCTTGCCAAG CGCAGTCGCA GTGGTAAATT CCGCCTCGTG 
ACCAAGTTTA AAAAGGAGAA AAACAACAAG AACAAAGAAG CTCACAGTAG CCTGGGAGCC 
CCGGTTCACC TCTGGGGGAC AGAGGAGGTT GCTGCCTGGC TGGAGCACCT CAGTCTCTGT 
GAGTATAAGG ACATCTTCAC ACGGCACGAC ATCCGGGGCT CTGAGCTCCT GCACCTGGAG 
CGGAGGGACC TCAAGGACCT GGGCGTGACC AAGGTGGGCC ACATGAAGAG GATCCTGTGT 
GGCATCAAGG AGCTGAGCCG CAGCGCCCCC GCCGTCGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860627.1
CDS	472..3996
Translation	MGEPEVLTCYKQTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_017005138.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005138.2

ORF Insert Sequence:

ATGGGGGAAC CTGAGGTCCT AACATGTTAC AAACAGACCA TCATCAAAGA GGGGATGCTG 
ACCAAACAGA ACAATTCATT CCAGCGATCA AAAAGGAGAT ACTTTAAGCT TCGAGGGCGA 
ACGCTTTACT ATGCCAAAAC GGCAAAGTCA ATCATATTTG ATGAGGTGGA TCTGACAGAT 
GCCAGCGTAG CTGAATCCAG TACCAAAAAC GTCAACAACA GTTTTACGGT CATAACTCCA 
TGCAGGAAGC TCATCTTGTG TGCTGATAAC AGAAAAGAAA TGGAAGATTG GATTGCAGCA 
TTAAAGACTG TGCAGAACAG GGAGCACTTT GAGCCCACCC AGTACAGCAT GGACCACTTC 
TCAGGGATGC ACAATTGGTA CGCCTGTTCC CACGCGAGGC CGACCTACTG CAATGTGTGC 
CGTGAGGCTC TGTCTGGGGT CACGTCGCAC GGGCTGTCCT GCGAGGTGTG CAAATTTAAG 
GCCCACAAGC GCTGTGCTGT GCGTGCAACC AATAACTGCA AGTGGACCAC ACTGGCCTCG 
ATCGGGAAGG ACATCATTGA AGATGCAGAT GGGATTGCAA TGCCCCACCA GTGGTTGGAA 
GGAAACCTAC CTGTGAGCGC CAAGTGCACT GTGTGCGACA AGACCTGTGG CAGTGTGCTG 
CGCCTGCAGG ACTGGCGCTG CCTCTGGTGC AAGGCCATGG TTCACACATC GTGTAAAGAA 
TCCTTGCTGA CCAAGTGCCC ACTTGGCCTG TGCAAAGTGT CAGTCATCCC ACCCACGGCT 
CTCAACAGCA TCGACTCCGA TGGGTTCTGG AAGGCCAGCT GTCCTCCTTC TTGCACAAGC 
CCACTGTTGG TCTTCGTCAA TTCAAAAAGT GGGGACAACC AGGGTGTGAA GTTCCTCAGA 
AGATTCAAAC AGCTACTAAA CCCCGCCCAG GTCTTCGACC TCATGAACGG AGGCCCACAC 
CTCGGCTTAC GGTTATTCCA GAAGTTTGAC ACATTCCGGA TTCTGGTTTG TGGCGGGGAT 
GGAAGTGTTG GCTGGGTCCT CTCCGAAATC GACAGCCTCA ACCTTCATAA ACAGTGTCAG 
CTGGGAGTGC TGCCGCTCGG CACAGGGAAC GACTTGGCCC GAGTACTGGG CTGGGGCTCA 
GCCTGCGATG ACGACACCCA GCTCCCCCAG ATCTTGGAGA AGTTGGAGAG AGCCAGCACC 
AAGATGCTGG ACAGGTGGAG CGTCATGGCA TACGAGGCCA AGCTCCCCCG GCAGGCCTCC 
TCCTCTACCG TCACCGAAGA CTTCAGCGAG GATTCCGAGG TACAGCAGAT TCTCTTCTAT 
GAAGACTCGG TTGCAGCCCA CCTTTCTAAA ATCCTCACCT CGGACCAGCA CTCGGTGGTC 
ATCTCCTCGG CCAAAGTGCT CTGTGAGACG GTGAAGGACT TCGTGGCACG GGTGGGGAAG 
GCCTATGAGA AGACGACCGA GAGCTCGGAG GAGTCAGAGG TCATGGCCAA GAAGTGCTCT 
GTCCTGAAAG AGAAGCTGGA TTCCCTTCTC AAGACCTTGG ACGATGAGTC CCAGGCCTCG 
TCCTCTCTGC CCAACCCGCC CCCCACCATT GCCGAGGAGG CTGAAGATGG AGATGGGTCG 
GGCAGCATCT GCGGTTCCAC CGGAGACCGC TTGGTGGCAT CAGCTTGCCC GGCCCGGCCG 
CAGATATTCC GGCCTCGAGA ACAGCTCATG CTGAGAGCCA ACAGCCTGAA GAAAGCAATT 
CGTCAGATCA TAGAACACAC AGAAAAAGCT GTCGATGAGC AGAATGCCCA GACCCAGGAG 
CAGGAGGGCT TCGTCCTGGG CCTCTCTGAG TCAGAGGAGA AGATGGACCA CAGAGTGTGC 
CCACCACTGT CCCACAGCGA GAGCTTCGGG GTCCCCAAGG GGAGGAGCCA GCGCAAAGTG 
TCGAAATCTC CGTGTGAAAA GCTGATCAGC AAAGGGAGTC TGTCCCTAGG CAGTTCTGCT 
TCCCTTCCGC CCCAGCCGGG AAGCCGGGAC GGCCTGCCTG CGCTCAACAC CAAGATCCTG 
TACCCAAATG TCCGGGCTGG AATGTCTGGT TCCTTACCCG GTGGCTCAGT CATCAGTCGC 
CTGTTAATTA ATGCTGATCC CTTCAACTCT GAACCAGAAA CCCTAGAGTA TTACACGGAG 
AAATGTGTCA TGAACAACTA TTTTGGCATT GGCCTGGATG CGAAGATATC CCTGGACTTT 
AACAACAAGC GCGATGAGCA CCCAGAGAAG TGCAGGAGCC GAACCAAGAA CATGATGTGG 
TATGGAGTTC TTGGAACCAA AGAGTTGCTG CACAGAACCT ACAAGAACCT GGAGCAAAAG 
GTCTTGCTGG AGTGTGACGG GCGACCCATC CCACTCCCCA GTCTTCAGGG AATTGCTGTC 
CTTAACATTC CCAGCTATGC CGGAGGAACC AACTTCTGGG GGGGTACCAA GGAAGATGAT 
ACTTTCGCAG CTCCATCATT CGATGACAAG ATTCTGGAGG TGGTCGCCGT GTTCGGCAGC 
ATGCAGATGG CCGTCTCTCG AGTCATCAGG CTACAGCATC ATCGGATCGC CCAGTGTCGC 
ACGGTGAAGA TCTCCATCCT TGGGGATGAG GGCGTGCCTG TGCAGGTGGA CGGAGAGGCC 
TGGGTCCAGC CGCCAGGGTA CATTCGGATT GTCCACAAGA ACCGGGCACA GACACTGACC 
AGAGACAGGG CATTTGAGAG CACCCTGAAG TCCTGGGAAG ACAAGCAGAA GTGCGAGCTG 
CCCCGCCCTC CATCCTGTTC CCTGCACCCG GAGATGCTGT CCGAGGAGGA GGCCACCCAG 
ATGGACCAGT TTGGGCAGGC AGCAGGGGTC CTCATTCACA GTATCCGAGA AATAGCTCAG 
TCTCACCGGG ACATGGAGCA GGAACTGGCC CACGCCGTCA ATGCCAGCTC CAAGTCCATG 
GACCGTGTGT ATGGCAAGCC CAGAACCACA GAGGGGCTCA ACTGCAGCTT CGTCCTGGAA 
ATGGTGAATA ACTTCAGAGC TCTGCGCAGT GAGACGGAGC TGCTGCTGTC TGGGAAGATG 
GCCCTGCAGC TGGATCCGCC TCAGAAGGAG CAGCTGGGGA GTGCTCTTGC CGAGATGGAC 
CGACAGCTCA GGAGGCTGGC AGACACCCCG TGGCTCTGCC AGTCCGCAGA GCCCGGCGAC 
GAAGAGAGTG TGATGCTGGA TCTTGCCAAG CGCAGTCGCA GTGGTAAATT CCGCCTCGTG 
ACCAAGTTTA AAAAGGAGAA AAACAACAAG AACAAAGAAG CTCACAGTAG CCTGGGAGCC 
CCGGTTCACC TCTGGGGGAC AGAGGAGGTT GCTGCCTGGC TGGAGCACCT CAGTCTCTGT 
GAGTATAAGG ACATCTTCAC ACGGCACGAC ATCCGGGGCT CTGAGCTCCT GCACCTGGAG 
CGGAGGGACC TCAAGGACCT GGGCGTGACC AAGGTGGGCC ACATGAAGAG GATCCTGTGT 
GGCATCAAGG AGCTGAGCCG CAGCGCCCCC GCCGTCGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_017005138.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860627.1 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X3, mRNA.

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CloneID	OHu61928
Clone ID Related Accession (Same CDS sequence)	XM_017005138.2 , XM_017005138.1
Accession Version	XM_017005138.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAC CTGAGGTCCT AACATGTTAC AAACAGACCA TCATCAAAGA GGGGATGCTG 
ACCAAACAGA ACAATTCATT CCAGCGATCA AAAAGGAGAT ACTTTAAGCT TCGAGGGCGA 
ACGCTTTACT ATGCCAAAAC GGCAAAGTCA ATCATATTTG ATGAGGTGGA TCTGACAGAT 
GCCAGCGTAG CTGAATCCAG TACCAAAAAC GTCAACAACA GTTTTACGGT CATAACTCCA 
TGCAGGAAGC TCATCTTGTG TGCTGATAAC AGAAAAGAAA TGGAAGATTG GATTGCAGCA 
TTAAAGACTG TGCAGAACAG GGAGCACTTT GAGCCCACCC AGTACAGCAT GGACCACTTC 
TCAGGGATGC ACAATTGGTA CGCCTGTTCC CACGCGAGGC CGACCTACTG CAATGTGTGC 
CGTGAGGCTC TGTCTGGGGT CACGTCGCAC GGGCTGTCCT GCGAGGTGTG CAAATTTAAG 
GCCCACAAGC GCTGTGCTGT GCGTGCAACC AATAACTGCA AGTGGACCAC ACTGGCCTCG 
ATCGGGAAGG ACATCATTGA AGATGCAGAT GGGATTGCAA TGCCCCACCA GTGGTTGGAA 
GGAAACCTAC CTGTGAGCGC CAAGTGCACT GTGTGCGACA AGACCTGTGG CAGTGTGCTG 
CGCCTGCAGG ACTGGCGCTG CCTCTGGTGC AAGGCCATGG TTCACACATC GTGTAAAGAA 
TCCTTGCTGA CCAAGTGCCC ACTTGGCCTG TGCAAAGTGT CAGTCATCCC ACCCACGGCT 
CTCAACAGCA TCGACTCCGA TGGGTTCTGG AAGGCCAGCT GTCCTCCTTC TTGCACAAGC 
CCACTGTTGG TCTTCGTCAA TTCAAAAAGT GGGGACAACC AGGGTGTGAA GTTCCTCAGA 
AGATTCAAAC AGCTACTAAA CCCCGCCCAG GTCTTCGACC TCATGAACGG AGGCCCACAC 
CTCGGCTTAC GGTTATTCCA GAAGTTTGAC ACATTCCGGA TTCTGGTTTG TGGCGGGGAT 
GGAAGTGTTG GCTGGGTCCT CTCCGAAATC GACAGCCTCA ACCTTCATAA ACAGTGTCAG 
CTGGGAGTGC TGCCGCTCGG CACAGGGAAC GACTTGGCCC GAGTACTGGG CTGGGGCTCA 
GCCTGCGATG ACGACACCCA GCTCCCCCAG ATCTTGGAGA AGTTGGAGAG AGCCAGCACC 
AAGATGCTGG ACAGGTGGAG CGTCATGGCA TACGAGGCCA AGCTCCCCCG GCAGGCCTCC 
TCCTCTACCG TCACCGAAGA CTTCAGCGAG GATTCCGAGG TACAGCAGAT TCTCTTCTAT 
GAAGACTCGG TTGCAGCCCA CCTTTCTAAA ATCCTCACCT CGGACCAGCA CTCGGTGGTC 
ATCTCCTCGG CCAAAGTGCT CTGTGAGACG GTGAAGGACT TCGTGGCACG GGTGGGGAAG 
GCCTATGAGA AGACGACCGA GAGCTCGGAG GAGTCAGAGG TCATGGCCAA GAAGTGCTCT 
GTCCTGAAAG AGAAGCTGGA TTCCCTTCTC AAGACCTTGG ACGATGAGTC CCAGGCCTCG 
TCCTCTCTGC CCAACCCGCC CCCCACCATT GCCGAGGAGG CTGAAGATGG AGATGGGTCG 
GGCAGCATCT GCGGTTCCAC CGGAGACCGC TTGGTGGCAT CAGCTTGCCC GGCCCGGCCG 
CAGATATTCC GGCCTCGAGA ACAGCTCATG CTGAGAGCCA ACAGCCTGAA GAAAGCAATT 
CGTCAGATCA TAGAACACAC AGAAAAAGCT GTCGATGAGC AGAATGCCCA GACCCAGGAG 
CAGGAGGGCT TCGTCCTGGG CCTCTCTGAG TCAGAGGAGA AGATGGACCA CAGAGTGTGC 
CCACCACTGT CCCACAGCGA GAGCTTCGGG GTCCCCAAGG GGAGGAGCCA GCGCAAAGTG 
TCGAAATCTC CGTGTGAAAA GCTGATCAGC AAAGGGAGTC TGTCCCTAGG CAGTTCTGCT 
TCCCTTCCGC CCCAGCCGGG AAGCCGGGAC GGCCTGCCTG CGCTCAACAC CAAGATCCTG 
TACCCAAATG TCCGGGCTGG AATGTCTGGT TCCTTACCCG GTGGCTCAGT CATCAGTCGC 
CTGTTAATTA ATGCTGATCC CTTCAACTCT GAACCAGAAA CCCTAGAGTA TTACACGGAG 
AAATGTGTCA TGAACAACTA TTTTGGCATT GGCCTGGATG CGAAGATATC CCTGGACTTT 
AACAACAAGC GCGATGAGCA CCCAGAGAAG TGCAGGAGCC GAACCAAGAA CATGATGTGG 
TATGGAGTTC TTGGAACCAA AGAGTTGCTG CACAGAACCT ACAAGAACCT GGAGCAAAAG 
GTCTTGCTGG AGTGTGACGG GCGACCCATC CCACTCCCCA GTCTTCAGGG AATTGCTGTC 
CTTAACATTC CCAGCTATGC CGGAGGAACC AACTTCTGGG GGGGTACCAA GGAAGATGAT 
ACTTTCGCAG CTCCATCATT CGATGACAAG ATTCTGGAGG TGGTCGCCGT GTTCGGCAGC 
ATGCAGATGG CCGTCTCTCG AGTCATCAGG CTACAGCATC ATCGGATCGC CCAGTGTCGC 
ACGGTGAAGA TCTCCATCCT TGGGGATGAG GGCGTGCCTG TGCAGGTGGA CGGAGAGGCC 
TGGGTCCAGC CGCCAGGGTA CATTCGGATT GTCCACAAGA ACCGGGCACA GACACTGACC 
AGAGACAGGG CATTTGAGAG CACCCTGAAG TCCTGGGAAG ACAAGCAGAA GTGCGAGCTG 
CCCCGCCCTC CATCCTGTTC CCTGCACCCG GAGATGCTGT CCGAGGAGGA GGCCACCCAG 
ATGGACCAGT TTGGGCAGGC AGCAGGGGTC CTCATTCACA GTATCCGAGA AATAGCTCAG 
TCTCACCGGG ACATGGAGCA GGAACTGGCC CACGCCGTCA ATGCCAGCTC CAAGTCCATG 
GACCGTGTGT ATGGCAAGCC CAGAACCACA GAGGGGCTCA ACTGCAGCTT CGTCCTGGAA 
ATGGTGAATA ACTTCAGAGC TCTGCGCAGT GAGACGGAGC TGCTGCTGTC TGGGAAGATG 
GCCCTGCAGC TGGATCCGCC TCAGAAGGAG CAGCTGGGGA GTGCTCTTGC CGAGATGGAC 
CGACAGCTCA GGAGGCTGGC AGACACCCCG TGGCTCTGCC AGTCCGCAGA GCCCGGCGAC 
GAAGAGAGTG TGATGCTGGA TCTTGCCAAG CGCAGTCGCA GTGGTAAATT CCGCCTCGTG 
ACCAAGTTTA AAAAGGAGAA AAACAACAAG AACAAAGAAG CTCACAGTAG CCTGGGAGCC 
CCGGTTCACC TCTGGGGGAC AGAGGAGGTT GCTGCCTGGC TGGAGCACCT CAGTCTCTGT 
GAGTATAAGG ACATCTTCAC ACGGCACGAC ATCCGGGGCT CTGAGCTCCT GCACCTGGAG 
CGGAGGGACC TCAAGGACCT GGGCGTGACC AAGGTGGGCC ACATGAAGAG GATCCTGTGT 
GGCATCAAGG AGCTGAGCCG CAGCGCCCCC GCCGTCGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860627.1
CDS	471..3995
Translation	MGEPEVLTCYKQTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	XM_017005138.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005138.1

ORF Insert Sequence:

ATGGGGGAAC CTGAGGTCCT AACATGTTAC AAACAGACCA TCATCAAAGA GGGGATGCTG 
ACCAAACAGA ACAATTCATT CCAGCGATCA AAAAGGAGAT ACTTTAAGCT TCGAGGGCGA 
ACGCTTTACT ATGCCAAAAC GGCAAAGTCA ATCATATTTG ATGAGGTGGA TCTGACAGAT 
GCCAGCGTAG CTGAATCCAG TACCAAAAAC GTCAACAACA GTTTTACGGT CATAACTCCA 
TGCAGGAAGC TCATCTTGTG TGCTGATAAC AGAAAAGAAA TGGAAGATTG GATTGCAGCA 
TTAAAGACTG TGCAGAACAG GGAGCACTTT GAGCCCACCC AGTACAGCAT GGACCACTTC 
TCAGGGATGC ACAATTGGTA CGCCTGTTCC CACGCGAGGC CGACCTACTG CAATGTGTGC 
CGTGAGGCTC TGTCTGGGGT CACGTCGCAC GGGCTGTCCT GCGAGGTGTG CAAATTTAAG 
GCCCACAAGC GCTGTGCTGT GCGTGCAACC AATAACTGCA AGTGGACCAC ACTGGCCTCG 
ATCGGGAAGG ACATCATTGA AGATGCAGAT GGGATTGCAA TGCCCCACCA GTGGTTGGAA 
GGAAACCTAC CTGTGAGCGC CAAGTGCACT GTGTGCGACA AGACCTGTGG CAGTGTGCTG 
CGCCTGCAGG ACTGGCGCTG CCTCTGGTGC AAGGCCATGG TTCACACATC GTGTAAAGAA 
TCCTTGCTGA CCAAGTGCCC ACTTGGCCTG TGCAAAGTGT CAGTCATCCC ACCCACGGCT 
CTCAACAGCA TCGACTCCGA TGGGTTCTGG AAGGCCAGCT GTCCTCCTTC TTGCACAAGC 
CCACTGTTGG TCTTCGTCAA TTCAAAAAGT GGGGACAACC AGGGTGTGAA GTTCCTCAGA 
AGATTCAAAC AGCTACTAAA CCCCGCCCAG GTCTTCGACC TCATGAACGG AGGCCCACAC 
CTCGGCTTAC GGTTATTCCA GAAGTTTGAC ACATTCCGGA TTCTGGTTTG TGGCGGGGAT 
GGAAGTGTTG GCTGGGTCCT CTCCGAAATC GACAGCCTCA ACCTTCATAA ACAGTGTCAG 
CTGGGAGTGC TGCCGCTCGG CACAGGGAAC GACTTGGCCC GAGTACTGGG CTGGGGCTCA 
GCCTGCGATG ACGACACCCA GCTCCCCCAG ATCTTGGAGA AGTTGGAGAG AGCCAGCACC 
AAGATGCTGG ACAGGTGGAG CGTCATGGCA TACGAGGCCA AGCTCCCCCG GCAGGCCTCC 
TCCTCTACCG TCACCGAAGA CTTCAGCGAG GATTCCGAGG TACAGCAGAT TCTCTTCTAT 
GAAGACTCGG TTGCAGCCCA CCTTTCTAAA ATCCTCACCT CGGACCAGCA CTCGGTGGTC 
ATCTCCTCGG CCAAAGTGCT CTGTGAGACG GTGAAGGACT TCGTGGCACG GGTGGGGAAG 
GCCTATGAGA AGACGACCGA GAGCTCGGAG GAGTCAGAGG TCATGGCCAA GAAGTGCTCT 
GTCCTGAAAG AGAAGCTGGA TTCCCTTCTC AAGACCTTGG ACGATGAGTC CCAGGCCTCG 
TCCTCTCTGC CCAACCCGCC CCCCACCATT GCCGAGGAGG CTGAAGATGG AGATGGGTCG 
GGCAGCATCT GCGGTTCCAC CGGAGACCGC TTGGTGGCAT CAGCTTGCCC GGCCCGGCCG 
CAGATATTCC GGCCTCGAGA ACAGCTCATG CTGAGAGCCA ACAGCCTGAA GAAAGCAATT 
CGTCAGATCA TAGAACACAC AGAAAAAGCT GTCGATGAGC AGAATGCCCA GACCCAGGAG 
CAGGAGGGCT TCGTCCTGGG CCTCTCTGAG TCAGAGGAGA AGATGGACCA CAGAGTGTGC 
CCACCACTGT CCCACAGCGA GAGCTTCGGG GTCCCCAAGG GGAGGAGCCA GCGCAAAGTG 
TCGAAATCTC CGTGTGAAAA GCTGATCAGC AAAGGGAGTC TGTCCCTAGG CAGTTCTGCT 
TCCCTTCCGC CCCAGCCGGG AAGCCGGGAC GGCCTGCCTG CGCTCAACAC CAAGATCCTG 
TACCCAAATG TCCGGGCTGG AATGTCTGGT TCCTTACCCG GTGGCTCAGT CATCAGTCGC 
CTGTTAATTA ATGCTGATCC CTTCAACTCT GAACCAGAAA CCCTAGAGTA TTACACGGAG 
AAATGTGTCA TGAACAACTA TTTTGGCATT GGCCTGGATG CGAAGATATC CCTGGACTTT 
AACAACAAGC GCGATGAGCA CCCAGAGAAG TGCAGGAGCC GAACCAAGAA CATGATGTGG 
TATGGAGTTC TTGGAACCAA AGAGTTGCTG CACAGAACCT ACAAGAACCT GGAGCAAAAG 
GTCTTGCTGG AGTGTGACGG GCGACCCATC CCACTCCCCA GTCTTCAGGG AATTGCTGTC 
CTTAACATTC CCAGCTATGC CGGAGGAACC AACTTCTGGG GGGGTACCAA GGAAGATGAT 
ACTTTCGCAG CTCCATCATT CGATGACAAG ATTCTGGAGG TGGTCGCCGT GTTCGGCAGC 
ATGCAGATGG CCGTCTCTCG AGTCATCAGG CTACAGCATC ATCGGATCGC CCAGTGTCGC 
ACGGTGAAGA TCTCCATCCT TGGGGATGAG GGCGTGCCTG TGCAGGTGGA CGGAGAGGCC 
TGGGTCCAGC CGCCAGGGTA CATTCGGATT GTCCACAAGA ACCGGGCACA GACACTGACC 
AGAGACAGGG CATTTGAGAG CACCCTGAAG TCCTGGGAAG ACAAGCAGAA GTGCGAGCTG 
CCCCGCCCTC CATCCTGTTC CCTGCACCCG GAGATGCTGT CCGAGGAGGA GGCCACCCAG 
ATGGACCAGT TTGGGCAGGC AGCAGGGGTC CTCATTCACA GTATCCGAGA AATAGCTCAG 
TCTCACCGGG ACATGGAGCA GGAACTGGCC CACGCCGTCA ATGCCAGCTC CAAGTCCATG 
GACCGTGTGT ATGGCAAGCC CAGAACCACA GAGGGGCTCA ACTGCAGCTT CGTCCTGGAA 
ATGGTGAATA ACTTCAGAGC TCTGCGCAGT GAGACGGAGC TGCTGCTGTC TGGGAAGATG 
GCCCTGCAGC TGGATCCGCC TCAGAAGGAG CAGCTGGGGA GTGCTCTTGC CGAGATGGAC 
CGACAGCTCA GGAGGCTGGC AGACACCCCG TGGCTCTGCC AGTCCGCAGA GCCCGGCGAC 
GAAGAGAGTG TGATGCTGGA TCTTGCCAAG CGCAGTCGCA GTGGTAAATT CCGCCTCGTG 
ACCAAGTTTA AAAAGGAGAA AAACAACAAG AACAAAGAAG CTCACAGTAG CCTGGGAGCC 
CCGGTTCACC TCTGGGGGAC AGAGGAGGTT GCTGCCTGGC TGGAGCACCT CAGTCTCTGT 
GAGTATAAGG ACATCTTCAC ACGGCACGAC ATCCGGGGCT CTGAGCTCCT GCACCTGGAG 
CGGAGGGACC TCAAGGACCT GGGCGTGACC AAGGTGGGCC ACATGAAGAG GATCCTGTGT 
GGCATCAAGG AGCTGAGCCG CAGCGCCCCC GCCGTCGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( NM_152879.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_690618.2 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 2, mRNA.

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CloneID	OHu17931
Clone ID Related Accession (Same CDS sequence)	NM_152879.2 , NM_152879.3
Accession Version	NM_152879.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC013726.7, D63479.3 and BC006561.2. This sequence is a reference standard in the RefSeqGene project. On Nov 27, 2002 this sequence version replaced NM_152879.1. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB078966.1, SRR1660807.244009.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGGCGGGCGC CCCTCCGCCG GGTCCCCCGC AACCGCCTCC GCCGCCGCCG 
CCCGAGGAGT CGTCCGACAG CGAGCCCGAG GCGGAGCCCG GCTCCCCACA GAAGCTCATC 
CGCAAGGTGT CCACGTCGGG TCAGATCCGA CAGAAGACCA TCATCAAAGA GGGGATGCTG 
ACCAAACAGA ACAATTCATT CCAGCGATCA AAAAGGAGAT ACTTTAAGCT TCGAGGGCGA 
ACGCTTTACT ATGCCAAAAC GGCAAAGTCA ATCATATTTG ATGAGGTGGA TCTGACAGAT 
GCCAGCGTAG CTGAATCCAG TACCAAAAAC GTCAACAACA GTTTTACGGT CATAACTCCA 
TGCAGGAAGC TCATCTTGTG TGCTGATAAC AGAAAAGAAA TGGAAGATTG GATTGCAGCA 
TTAAAGACTG TGCAGAACAG GGAGCACTTT GAGCCCACCC AGTACAGCAT GGACCACTTC 
TCAGGGATGC ACAATTGGTA CGCCTGTTCC CACGCGAGGC CGACCTACTG CAATGTGTGC 
CGTGAGGCTC TGTCTGGGGT CACGTCGCAC GGGCTGTCCT GCGAGGTGTG CAAATTTAAG 
GCCCACAAGC GCTGTGCTGT GCGTGCAACC AATAACTGCA AGTGGACCAC ACTGGCCTCG 
ATCGGGAAGG ACATCATTGA AGATGCAGAT GGGATTGCAA TGCCCCACCA GTGGTTGGAA 
GGAAACCTAC CTGTGAGCGC CAAGTGCACT GTGTGCGACA AGACCTGTGG CAGTGTGCTG 
CGCCTGCAGG ACTGGCGCTG CCTCTGGTGC AAGGCCATGG TTCACACATC GTGTAAAGAA 
TCCTTGCTGA CCAAGTGCCC ACTTGGCCTG TGCAAAGTGT CAGTCATCCC ACCCACGGCT 
CTCAACAGCA TCGACTCCGA TGGGTTCTGG AAGGCCAGCT GTCCTCCTTC TTGCACAAGC 
CCACTGTTGG TCTTCGTCAA TTCAAAAAGT GGGGACAACC AGGGTGTGAA GTTCCTCAGA 
AGATTCAAAC AGCTACTAAA CCCCGCCCAG GTCTTCGACC TCATGAACGG AGGCCCACAC 
CTCGGCTTAC GGTTATTCCA GAAGTTTGAC ACATTCCGGA TTCTGGTTTG TGGCGGGGAT 
GGAAGTGTTG GCTGGGTCCT CTCCGAAATC GACAGCCTCA ACCTTCATAA ACAGTGTCAG 
CTGGGAGTGC TGCCGCTCGG CACAGGGAAC GACTTGGCCC GAGTACTGGG CTGGGGCTCA 
GCCTGCGATG ACGACACCCA GCTCCCCCAG ATCTTGGAGA AGTTGGAGAG AGCCAGCACC 
AAGATGCTGG ACAGGTGGAG CGTCATGGCA TACGAGGCCA AGCTCCCCCG GCAGGCCTCC 
TCCTCTACCG TCACCGAAGA CTTCAGCGAG GATTCCGAGG TACAGCAGAT TCTCTTCTAT 
GAAGACTCGG TTGCAGCCCA CCTTTCTAAA ATCCTCACCT CGGACCAGCA CTCGGTGGTC 
ATCTCCTCGG CCAAAGTGCT CTGTGAGACG GTGAAGGACT TCGTGGCACG GGTGGGGAAG 
GCCTATGAGA AGACGACCGA GAGCTCGGAG GAGTCAGAGG TCATGGCCAA GAAGTGCTCT 
GTCCTGAAAG AGAAGCTGGA TTCCCTTCTC AAGACCTTGG ACGATGAGTC CCAGGCCTCG 
TCCTCTCTGC CCAACCCGCC CCCCACCATT GCCGAGGAGG CTGAAGATGG AGATGGGTCG 
GGCAGCATCT GCGGTTCCAC CGGAGACCGC TTGGTGGCAT CAGCTTGCCC GGCCCGGCCG 
CAGATATTCC GGCCTCGAGA ACAGCTCATG CTGAGAGCCA ACAGCCTGAA GAAAGCAATT 
CGTCAGATCA TAGAACACAC AGAAAAAGCT GTCGATGAGC AGAATGCCCA GACCCAGGAG 
CAGGAGGGCT TCGTCCTGGG CCTCTCTGAG TCAGAGGAGA AGATGGACCA CAGAGTGTGC 
CCACCACTGT CCCACAGCGA GAGCTTCGGG GTCCCCAAGG GGAGGAGCCA GCGCAAAGTG 
TCGAAATCTC CGTGTGAAAA GCTGATCAGC AAAGGGAGTC TGTCCCTAGG CAGTTCTGCT 
TCCCTTCCGC CCCAGCCGGG AAGCCGGGAC GGCCTGCCTG CGCTCAACAC CAAGATCCTG 
TACCCAAATG TCCGGGCTGG AATGTCTGGT TCCTTACCCG GTGGCTCAGT CATCAGTCGC 
CTGTTAATTA ATGCTGATCC CTTCAACTCT GAACCAGAAA CCCTAGAGTA TTACACGGAG 
AAATGTGTCA TGAACAACTA TTTTGGCATT GGCCTGGATG CGAAGATATC CCTGGACTTT 
AACAACAAGC GCGATGAGCA CCCAGAGAAG TGCAGGAGCC GAACCAAGAA CATGATGTGG 
TATGGAGTTC TTGGAACCAA AGAGTTGCTG CACAGAACCT ACAAGAACCT GGAGCAAAAG 
GTCTTGCTGG AGTGTGACGG GCGACCCATC CCACTCCCCA GTCTTCAGGG AATTGCTGTC 
CTTAACATTC CCAGCTATGC CGGAGGAACC AACTTCTGGG GGGGTACCAA GGAAGATGAT 
ACTTTCGCAG CTCCATCATT CGATGACAAG ATTCTGGAGG TGGTCGCCGT GTTCGGCAGC 
ATGCAGATGG CCGTCTCTCG AGTCATCAGG CTACAGCATC ATCGGATCGC CCAGTGTCGC 
ACGGTGAAGA TCTCCATCCT TGGGGATGAG GGCGTGCCTG TGCAGGTGGA CGGAGAGGCC 
TGGGTCCAGC CGCCAGGGTA CATTCGGATT GTCCACAAGA ACCGGGCACA GACACTGACC 
AGAGACAGGG CATTTGAGAG CACCCTGAAG TCCTGGGAAG ACAAGCAGAA GTGCGAGCTG 
CCCCGCCCTC CATCCTGTTC CCTGCACCCG GAGATGCTGT CCGAGGAGGA GGCCACCCAG 
ATGGACCAGT TTGGGCAGGC AGCAGGGGTC CTCATTCACA GTATCCGAGA AATAGCTCAG 
TCTCACCGGG ACATGGAGCA GGAACTGGCC CACGCCGTCA ATGCCAGCTC CAAGTCCATG 
GACCGTGTGT ATGGCAAGCC CAGAACCACA GAGGGGCTCA ACTGCAGCTT CGTCCTGGAA 
ATGGTGAATA ACTTCAGAGC TCTGCGCAGT GAGACGGAGC TGCTGCTGTC TGGGAAGATG 
GCCCTGCAGC TGGATCCGCC TCAGAAGGAG CAGCTGGGGA GTGCTCTTGC CGAGATGGAC 
CGACAGCTCA GGAGGCTGGC AGACACCCCG TGGCTCTGCC AGTCCGCAGA GCCCGGCGAC 
GAAGAGAGTG TGATGCTGGA TCTTGCCAAG CGCAGTCGCA GTGGTAAATT CCGCCTCGTG 
ACCAAGTTTA AAAAGGAGAA AAACAACAAG AACAAAGAAG CTCACAGTAG CCTGGGAGCC 
CCGGTTCACC TCTGGGGGAC AGAGGAGGTT GCTGCCTGGC TGGAGCACCT CAGTCTCTGT 
GAGTATAAGG ACATCTTCAC ACGGCACGAC ATCCGGGGCT CTGAGCTCCT GCACCTGGAG 
CGGAGGGACC TCAAGGACCT GGGCGTGACC AAGGTGGGCC ACATGAAGAG GATCCTGTGT 
GGCATCAAGG AGCTGAGCCG CAGCGCCCCC GCCGTCGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_690618.2
CDS	13..3657
Translation	MAAAAGAPPPGPPQPPPPPPPEESSDSEPEAEPGSPQKLIRKVSTSGQIRQKTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	NM_152879.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152879.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGCGC CCCTCCGCCG GGTCCCCCGC AACCGCCTCC GCCGCCGCCG 
CCCGAGGAGT CGTCCGACAG CGAGCCCGAG GCGGAGCCCG GCTCCCCACA GAAGCTCATC 
CGCAAGGTGT CCACGTCGGG TCAGATCCGA CAGAAGACCA TCATCAAAGA GGGGATGCTG 
ACCAAACAGA ACAATTCATT CCAGCGATCA AAAAGGAGAT ACTTTAAGCT TCGAGGGCGA 
ACGCTTTACT ATGCCAAAAC GGCAAAGTCA ATCATATTTG ATGAGGTGGA TCTGACAGAT 
GCCAGCGTAG CTGAATCCAG TACCAAAAAC GTCAACAACA GTTTTACGGT CATAACTCCA 
TGCAGGAAGC TCATCTTGTG TGCTGATAAC AGAAAAGAAA TGGAAGATTG GATTGCAGCA 
TTAAAGACTG TGCAGAACAG GGAGCACTTT GAGCCCACCC AGTACAGCAT GGACCACTTC 
TCAGGGATGC ACAATTGGTA CGCCTGTTCC CACGCGAGGC CGACCTACTG CAATGTGTGC 
CGTGAGGCTC TGTCTGGGGT CACGTCGCAC GGGCTGTCCT GCGAGGTGTG CAAATTTAAG 
GCCCACAAGC GCTGTGCTGT GCGTGCAACC AATAACTGCA AGTGGACCAC ACTGGCCTCG 
ATCGGGAAGG ACATCATTGA AGATGCAGAT GGGATTGCAA TGCCCCACCA GTGGTTGGAA 
GGAAACCTAC CTGTGAGCGC CAAGTGCACT GTGTGCGACA AGACCTGTGG CAGTGTGCTG 
CGCCTGCAGG ACTGGCGCTG CCTCTGGTGC AAGGCCATGG TTCACACATC GTGTAAAGAA 
TCCTTGCTGA CCAAGTGCCC ACTTGGCCTG TGCAAAGTGT CAGTCATCCC ACCCACGGCT 
CTCAACAGCA TCGACTCCGA TGGGTTCTGG AAGGCCAGCT GTCCTCCTTC TTGCACAAGC 
CCACTGTTGG TCTTCGTCAA TTCAAAAAGT GGGGACAACC AGGGTGTGAA GTTCCTCAGA 
AGATTCAAAC AGCTACTAAA CCCCGCCCAG GTCTTCGACC TCATGAACGG AGGCCCACAC 
CTCGGCTTAC GGTTATTCCA GAAGTTTGAC ACATTCCGGA TTCTGGTTTG TGGCGGGGAT 
GGAAGTGTTG GCTGGGTCCT CTCCGAAATC GACAGCCTCA ACCTTCATAA ACAGTGTCAG 
CTGGGAGTGC TGCCGCTCGG CACAGGGAAC GACTTGGCCC GAGTACTGGG CTGGGGCTCA 
GCCTGCGATG ACGACACCCA GCTCCCCCAG ATCTTGGAGA AGTTGGAGAG AGCCAGCACC 
AAGATGCTGG ACAGGTGGAG CGTCATGGCA TACGAGGCCA AGCTCCCCCG GCAGGCCTCC 
TCCTCTACCG TCACCGAAGA CTTCAGCGAG GATTCCGAGG TACAGCAGAT TCTCTTCTAT 
GAAGACTCGG TTGCAGCCCA CCTTTCTAAA ATCCTCACCT CGGACCAGCA CTCGGTGGTC 
ATCTCCTCGG CCAAAGTGCT CTGTGAGACG GTGAAGGACT TCGTGGCACG GGTGGGGAAG 
GCCTATGAGA AGACGACCGA GAGCTCGGAG GAGTCAGAGG TCATGGCCAA GAAGTGCTCT 
GTCCTGAAAG AGAAGCTGGA TTCCCTTCTC AAGACCTTGG ACGATGAGTC CCAGGCCTCG 
TCCTCTCTGC CCAACCCGCC CCCCACCATT GCCGAGGAGG CTGAAGATGG AGATGGGTCG 
GGCAGCATCT GCGGTTCCAC CGGAGACCGC TTGGTGGCAT CAGCTTGCCC GGCCCGGCCG 
CAGATATTCC GGCCTCGAGA ACAGCTCATG CTGAGAGCCA ACAGCCTGAA GAAAGCAATT 
CGTCAGATCA TAGAACACAC AGAAAAAGCT GTCGATGAGC AGAATGCCCA GACCCAGGAG 
CAGGAGGGCT TCGTCCTGGG CCTCTCTGAG TCAGAGGAGA AGATGGACCA CAGAGTGTGC 
CCACCACTGT CCCACAGCGA GAGCTTCGGG GTCCCCAAGG GGAGGAGCCA GCGCAAAGTG 
TCGAAATCTC CGTGTGAAAA GCTGATCAGC AAAGGGAGTC TGTCCCTAGG CAGTTCTGCT 
TCCCTTCCGC CCCAGCCGGG AAGCCGGGAC GGCCTGCCTG CGCTCAACAC CAAGATCCTG 
TACCCAAATG TCCGGGCTGG AATGTCTGGT TCCTTACCCG GTGGCTCAGT CATCAGTCGC 
CTGTTAATTA ATGCTGATCC CTTCAACTCT GAACCAGAAA CCCTAGAGTA TTACACGGAG 
AAATGTGTCA TGAACAACTA TTTTGGCATT GGCCTGGATG CGAAGATATC CCTGGACTTT 
AACAACAAGC GCGATGAGCA CCCAGAGAAG TGCAGGAGCC GAACCAAGAA CATGATGTGG 
TATGGAGTTC TTGGAACCAA AGAGTTGCTG CACAGAACCT ACAAGAACCT GGAGCAAAAG 
GTCTTGCTGG AGTGTGACGG GCGACCCATC CCACTCCCCA GTCTTCAGGG AATTGCTGTC 
CTTAACATTC CCAGCTATGC CGGAGGAACC AACTTCTGGG GGGGTACCAA GGAAGATGAT 
ACTTTCGCAG CTCCATCATT CGATGACAAG ATTCTGGAGG TGGTCGCCGT GTTCGGCAGC 
ATGCAGATGG CCGTCTCTCG AGTCATCAGG CTACAGCATC ATCGGATCGC CCAGTGTCGC 
ACGGTGAAGA TCTCCATCCT TGGGGATGAG GGCGTGCCTG TGCAGGTGGA CGGAGAGGCC 
TGGGTCCAGC CGCCAGGGTA CATTCGGATT GTCCACAAGA ACCGGGCACA GACACTGACC 
AGAGACAGGG CATTTGAGAG CACCCTGAAG TCCTGGGAAG ACAAGCAGAA GTGCGAGCTG 
CCCCGCCCTC CATCCTGTTC CCTGCACCCG GAGATGCTGT CCGAGGAGGA GGCCACCCAG 
ATGGACCAGT TTGGGCAGGC AGCAGGGGTC CTCATTCACA GTATCCGAGA AATAGCTCAG 
TCTCACCGGG ACATGGAGCA GGAACTGGCC CACGCCGTCA ATGCCAGCTC CAAGTCCATG 
GACCGTGTGT ATGGCAAGCC CAGAACCACA GAGGGGCTCA ACTGCAGCTT CGTCCTGGAA 
ATGGTGAATA ACTTCAGAGC TCTGCGCAGT GAGACGGAGC TGCTGCTGTC TGGGAAGATG 
GCCCTGCAGC TGGATCCGCC TCAGAAGGAG CAGCTGGGGA GTGCTCTTGC CGAGATGGAC 
CGACAGCTCA GGAGGCTGGC AGACACCCCG TGGCTCTGCC AGTCCGCAGA GCCCGGCGAC 
GAAGAGAGTG TGATGCTGGA TCTTGCCAAG CGCAGTCGCA GTGGTAAATT CCGCCTCGTG 
ACCAAGTTTA AAAAGGAGAA AAACAACAAG AACAAAGAAG CTCACAGTAG CCTGGGAGCC 
CCGGTTCACC TCTGGGGGAC AGAGGAGGTT GCTGCCTGGC TGGAGCACCT CAGTCTCTGT 
GAGTATAAGG ACATCTTCAC ACGGCACGAC ATCCGGGGCT CTGAGCTCCT GCACCTGGAG 
CGGAGGGACC TCAAGGACCT GGGCGTGACC AAGGTGGGCC ACATGAAGAG GATCCTGTGT 
GGCATCAAGG AGCTGAGCCG CAGCGCCCCC GCCGTCGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( NM_152879.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_690618.2 Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 2, mRNA.

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CloneID	OHu17931
Clone ID Related Accession (Same CDS sequence)	NM_152879.2 , NM_152879.3
Accession Version	NM_152879.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	diacylglycerol kinase delta isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC013726.7, D63479.3, BC006561.2 and AC019221.4. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_152879.2. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.183865.1, SRR1803613.214186.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGGCGGGCGC CCCTCCGCCG GGTCCCCCGC AACCGCCTCC GCCGCCGCCG 
CCCGAGGAGT CGTCCGACAG CGAGCCCGAG GCGGAGCCCG GCTCCCCACA GAAGCTCATC 
CGCAAGGTGT CCACGTCGGG TCAGATCCGA CAGAAGACCA TCATCAAAGA GGGGATGCTG 
ACCAAACAGA ACAATTCATT CCAGCGATCA AAAAGGAGAT ACTTTAAGCT TCGAGGGCGA 
ACGCTTTACT ATGCCAAAAC GGCAAAGTCA ATCATATTTG ATGAGGTGGA TCTGACAGAT 
GCCAGCGTAG CTGAATCCAG TACCAAAAAC GTCAACAACA GTTTTACGGT CATAACTCCA 
TGCAGGAAGC TCATCTTGTG TGCTGATAAC AGAAAAGAAA TGGAAGATTG GATTGCAGCA 
TTAAAGACTG TGCAGAACAG GGAGCACTTT GAGCCCACCC AGTACAGCAT GGACCACTTC 
TCAGGGATGC ACAATTGGTA CGCCTGTTCC CACGCGAGGC CGACCTACTG CAATGTGTGC 
CGTGAGGCTC TGTCTGGGGT CACGTCGCAC GGGCTGTCCT GCGAGGTGTG CAAATTTAAG 
GCCCACAAGC GCTGTGCTGT GCGTGCAACC AATAACTGCA AGTGGACCAC ACTGGCCTCG 
ATCGGGAAGG ACATCATTGA AGATGCAGAT GGGATTGCAA TGCCCCACCA GTGGTTGGAA 
GGAAACCTAC CTGTGAGCGC CAAGTGCACT GTGTGCGACA AGACCTGTGG CAGTGTGCTG 
CGCCTGCAGG ACTGGCGCTG CCTCTGGTGC AAGGCCATGG TTCACACATC GTGTAAAGAA 
TCCTTGCTGA CCAAGTGCCC ACTTGGCCTG TGCAAAGTGT CAGTCATCCC ACCCACGGCT 
CTCAACAGCA TCGACTCCGA TGGGTTCTGG AAGGCCAGCT GTCCTCCTTC TTGCACAAGC 
CCACTGTTGG TCTTCGTCAA TTCAAAAAGT GGGGACAACC AGGGTGTGAA GTTCCTCAGA 
AGATTCAAAC AGCTACTAAA CCCCGCCCAG GTCTTCGACC TCATGAACGG AGGCCCACAC 
CTCGGCTTAC GGTTATTCCA GAAGTTTGAC ACATTCCGGA TTCTGGTTTG TGGCGGGGAT 
GGAAGTGTTG GCTGGGTCCT CTCCGAAATC GACAGCCTCA ACCTTCATAA ACAGTGTCAG 
CTGGGAGTGC TGCCGCTCGG CACAGGGAAC GACTTGGCCC GAGTACTGGG CTGGGGCTCA 
GCCTGCGATG ACGACACCCA GCTCCCCCAG ATCTTGGAGA AGTTGGAGAG AGCCAGCACC 
AAGATGCTGG ACAGGTGGAG CGTCATGGCA TACGAGGCCA AGCTCCCCCG GCAGGCCTCC 
TCCTCTACCG TCACCGAAGA CTTCAGCGAG GATTCCGAGG TACAGCAGAT TCTCTTCTAT 
GAAGACTCGG TTGCAGCCCA CCTTTCTAAA ATCCTCACCT CGGACCAGCA CTCGGTGGTC 
ATCTCCTCGG CCAAAGTGCT CTGTGAGACG GTGAAGGACT TCGTGGCACG GGTGGGGAAG 
GCCTATGAGA AGACGACCGA GAGCTCGGAG GAGTCAGAGG TCATGGCCAA GAAGTGCTCT 
GTCCTGAAAG AGAAGCTGGA TTCCCTTCTC AAGACCTTGG ACGATGAGTC CCAGGCCTCG 
TCCTCTCTGC CCAACCCGCC CCCCACCATT GCCGAGGAGG CTGAAGATGG AGATGGGTCG 
GGCAGCATCT GCGGTTCCAC CGGAGACCGC TTGGTGGCAT CAGCTTGCCC GGCCCGGCCG 
CAGATATTCC GGCCTCGAGA ACAGCTCATG CTGAGAGCCA ACAGCCTGAA GAAAGCAATT 
CGTCAGATCA TAGAACACAC AGAAAAAGCT GTCGATGAGC AGAATGCCCA GACCCAGGAG 
CAGGAGGGCT TCGTCCTGGG CCTCTCTGAG TCAGAGGAGA AGATGGACCA CAGAGTGTGC 
CCACCACTGT CCCACAGCGA GAGCTTCGGG GTCCCCAAGG GGAGGAGCCA GCGCAAAGTG 
TCGAAATCTC CGTGTGAAAA GCTGATCAGC AAAGGGAGTC TGTCCCTAGG CAGTTCTGCT 
TCCCTTCCGC CCCAGCCGGG AAGCCGGGAC GGCCTGCCTG CGCTCAACAC CAAGATCCTG 
TACCCAAATG TCCGGGCTGG AATGTCTGGT TCCTTACCCG GTGGCTCAGT CATCAGTCGC 
CTGTTAATTA ATGCTGATCC CTTCAACTCT GAACCAGAAA CCCTAGAGTA TTACACGGAG 
AAATGTGTCA TGAACAACTA TTTTGGCATT GGCCTGGATG CGAAGATATC CCTGGACTTT 
AACAACAAGC GCGATGAGCA CCCAGAGAAG TGCAGGAGCC GAACCAAGAA CATGATGTGG 
TATGGAGTTC TTGGAACCAA AGAGTTGCTG CACAGAACCT ACAAGAACCT GGAGCAAAAG 
GTCTTGCTGG AGTGTGACGG GCGACCCATC CCACTCCCCA GTCTTCAGGG AATTGCTGTC 
CTTAACATTC CCAGCTATGC CGGAGGAACC AACTTCTGGG GGGGTACCAA GGAAGATGAT 
ACTTTCGCAG CTCCATCATT CGATGACAAG ATTCTGGAGG TGGTCGCCGT GTTCGGCAGC 
ATGCAGATGG CCGTCTCTCG AGTCATCAGG CTACAGCATC ATCGGATCGC CCAGTGTCGC 
ACGGTGAAGA TCTCCATCCT TGGGGATGAG GGCGTGCCTG TGCAGGTGGA CGGAGAGGCC 
TGGGTCCAGC CGCCAGGGTA CATTCGGATT GTCCACAAGA ACCGGGCACA GACACTGACC 
AGAGACAGGG CATTTGAGAG CACCCTGAAG TCCTGGGAAG ACAAGCAGAA GTGCGAGCTG 
CCCCGCCCTC CATCCTGTTC CCTGCACCCG GAGATGCTGT CCGAGGAGGA GGCCACCCAG 
ATGGACCAGT TTGGGCAGGC AGCAGGGGTC CTCATTCACA GTATCCGAGA AATAGCTCAG 
TCTCACCGGG ACATGGAGCA GGAACTGGCC CACGCCGTCA ATGCCAGCTC CAAGTCCATG 
GACCGTGTGT ATGGCAAGCC CAGAACCACA GAGGGGCTCA ACTGCAGCTT CGTCCTGGAA 
ATGGTGAATA ACTTCAGAGC TCTGCGCAGT GAGACGGAGC TGCTGCTGTC TGGGAAGATG 
GCCCTGCAGC TGGATCCGCC TCAGAAGGAG CAGCTGGGGA GTGCTCTTGC CGAGATGGAC 
CGACAGCTCA GGAGGCTGGC AGACACCCCG TGGCTCTGCC AGTCCGCAGA GCCCGGCGAC 
GAAGAGAGTG TGATGCTGGA TCTTGCCAAG CGCAGTCGCA GTGGTAAATT CCGCCTCGTG 
ACCAAGTTTA AAAAGGAGAA AAACAACAAG AACAAAGAAG CTCACAGTAG CCTGGGAGCC 
CCGGTTCACC TCTGGGGGAC AGAGGAGGTT GCTGCCTGGC TGGAGCACCT CAGTCTCTGT 
GAGTATAAGG ACATCTTCAC ACGGCACGAC ATCCGGGGCT CTGAGCTCCT GCACCTGGAG 
CGGAGGGACC TCAAGGACCT GGGCGTGACC AAGGTGGGCC ACATGAAGAG GATCCTGTGT 
GGCATCAAGG AGCTGAGCCG CAGCGCCCCC GCCGTCGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_690618.2
CDS	26..3670
Translation	MAAAAGAPPPGPPQPPPPPPPEESSDSEPEAEPGSPQKLIRKVSTSGQIRQKTIIKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDADGIAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLLTKCPLGLCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYEAKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSEESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLPNPPPTIAEEAEDGDGSGSICGSTGDRLVASACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQEGFVLGLSESEEKMDHRVCPPLSHSESFGVPKGRSQRKVSKSPCEKLISKGSLSLGSSASLPPQPGSRDGLPALNTKILYPNVRAGMSGSLPGGSVISRLLINADPFNSEPETLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIRLQHHRIAQCRTVKISILGDEGVPVQVDGEAWVQPPGYIRIVHKNRAQTLTRDRAFESTLKSWEDKQKCELPRPPSCSLHPEMLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAHAVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALRSETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLADTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEKNNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSAPAVEA

Target ORF information:

RefSeq Version	NM_152879.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152879.3

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGCGC CCCTCCGCCG GGTCCCCCGC AACCGCCTCC GCCGCCGCCG 
CCCGAGGAGT CGTCCGACAG CGAGCCCGAG GCGGAGCCCG GCTCCCCACA GAAGCTCATC 
CGCAAGGTGT CCACGTCGGG TCAGATCCGA CAGAAGACCA TCATCAAAGA GGGGATGCTG 
ACCAAACAGA ACAATTCATT CCAGCGATCA AAAAGGAGAT ACTTTAAGCT TCGAGGGCGA 
ACGCTTTACT ATGCCAAAAC GGCAAAGTCA ATCATATTTG ATGAGGTGGA TCTGACAGAT 
GCCAGCGTAG CTGAATCCAG TACCAAAAAC GTCAACAACA GTTTTACGGT CATAACTCCA 
TGCAGGAAGC TCATCTTGTG TGCTGATAAC AGAAAAGAAA TGGAAGATTG GATTGCAGCA 
TTAAAGACTG TGCAGAACAG GGAGCACTTT GAGCCCACCC AGTACAGCAT GGACCACTTC 
TCAGGGATGC ACAATTGGTA CGCCTGTTCC CACGCGAGGC CGACCTACTG CAATGTGTGC 
CGTGAGGCTC TGTCTGGGGT CACGTCGCAC GGGCTGTCCT GCGAGGTGTG CAAATTTAAG 
GCCCACAAGC GCTGTGCTGT GCGTGCAACC AATAACTGCA AGTGGACCAC ACTGGCCTCG 
ATCGGGAAGG ACATCATTGA AGATGCAGAT GGGATTGCAA TGCCCCACCA GTGGTTGGAA 
GGAAACCTAC CTGTGAGCGC CAAGTGCACT GTGTGCGACA AGACCTGTGG CAGTGTGCTG 
CGCCTGCAGG ACTGGCGCTG CCTCTGGTGC AAGGCCATGG TTCACACATC GTGTAAAGAA 
TCCTTGCTGA CCAAGTGCCC ACTTGGCCTG TGCAAAGTGT CAGTCATCCC ACCCACGGCT 
CTCAACAGCA TCGACTCCGA TGGGTTCTGG AAGGCCAGCT GTCCTCCTTC TTGCACAAGC 
CCACTGTTGG TCTTCGTCAA TTCAAAAAGT GGGGACAACC AGGGTGTGAA GTTCCTCAGA 
AGATTCAAAC AGCTACTAAA CCCCGCCCAG GTCTTCGACC TCATGAACGG AGGCCCACAC 
CTCGGCTTAC GGTTATTCCA GAAGTTTGAC ACATTCCGGA TTCTGGTTTG TGGCGGGGAT 
GGAAGTGTTG GCTGGGTCCT CTCCGAAATC GACAGCCTCA ACCTTCATAA ACAGTGTCAG 
CTGGGAGTGC TGCCGCTCGG CACAGGGAAC GACTTGGCCC GAGTACTGGG CTGGGGCTCA 
GCCTGCGATG ACGACACCCA GCTCCCCCAG ATCTTGGAGA AGTTGGAGAG AGCCAGCACC 
AAGATGCTGG ACAGGTGGAG CGTCATGGCA TACGAGGCCA AGCTCCCCCG GCAGGCCTCC 
TCCTCTACCG TCACCGAAGA CTTCAGCGAG GATTCCGAGG TACAGCAGAT TCTCTTCTAT 
GAAGACTCGG TTGCAGCCCA CCTTTCTAAA ATCCTCACCT CGGACCAGCA CTCGGTGGTC 
ATCTCCTCGG CCAAAGTGCT CTGTGAGACG GTGAAGGACT TCGTGGCACG GGTGGGGAAG 
GCCTATGAGA AGACGACCGA GAGCTCGGAG GAGTCAGAGG TCATGGCCAA GAAGTGCTCT 
GTCCTGAAAG AGAAGCTGGA TTCCCTTCTC AAGACCTTGG ACGATGAGTC CCAGGCCTCG 
TCCTCTCTGC CCAACCCGCC CCCCACCATT GCCGAGGAGG CTGAAGATGG AGATGGGTCG 
GGCAGCATCT GCGGTTCCAC CGGAGACCGC TTGGTGGCAT CAGCTTGCCC GGCCCGGCCG 
CAGATATTCC GGCCTCGAGA ACAGCTCATG CTGAGAGCCA ACAGCCTGAA GAAAGCAATT 
CGTCAGATCA TAGAACACAC AGAAAAAGCT GTCGATGAGC AGAATGCCCA GACCCAGGAG 
CAGGAGGGCT TCGTCCTGGG CCTCTCTGAG TCAGAGGAGA AGATGGACCA CAGAGTGTGC 
CCACCACTGT CCCACAGCGA GAGCTTCGGG GTCCCCAAGG GGAGGAGCCA GCGCAAAGTG 
TCGAAATCTC CGTGTGAAAA GCTGATCAGC AAAGGGAGTC TGTCCCTAGG CAGTTCTGCT 
TCCCTTCCGC CCCAGCCGGG AAGCCGGGAC GGCCTGCCTG CGCTCAACAC CAAGATCCTG 
TACCCAAATG TCCGGGCTGG AATGTCTGGT TCCTTACCCG GTGGCTCAGT CATCAGTCGC 
CTGTTAATTA ATGCTGATCC CTTCAACTCT GAACCAGAAA CCCTAGAGTA TTACACGGAG 
AAATGTGTCA TGAACAACTA TTTTGGCATT GGCCTGGATG CGAAGATATC CCTGGACTTT 
AACAACAAGC GCGATGAGCA CCCAGAGAAG TGCAGGAGCC GAACCAAGAA CATGATGTGG 
TATGGAGTTC TTGGAACCAA AGAGTTGCTG CACAGAACCT ACAAGAACCT GGAGCAAAAG 
GTCTTGCTGG AGTGTGACGG GCGACCCATC CCACTCCCCA GTCTTCAGGG AATTGCTGTC 
CTTAACATTC CCAGCTATGC CGGAGGAACC AACTTCTGGG GGGGTACCAA GGAAGATGAT 
ACTTTCGCAG CTCCATCATT CGATGACAAG ATTCTGGAGG TGGTCGCCGT GTTCGGCAGC 
ATGCAGATGG CCGTCTCTCG AGTCATCAGG CTACAGCATC ATCGGATCGC CCAGTGTCGC 
ACGGTGAAGA TCTCCATCCT TGGGGATGAG GGCGTGCCTG TGCAGGTGGA CGGAGAGGCC 
TGGGTCCAGC CGCCAGGGTA CATTCGGATT GTCCACAAGA ACCGGGCACA GACACTGACC 
AGAGACAGGG CATTTGAGAG CACCCTGAAG TCCTGGGAAG ACAAGCAGAA GTGCGAGCTG 
CCCCGCCCTC CATCCTGTTC CCTGCACCCG GAGATGCTGT CCGAGGAGGA GGCCACCCAG 
ATGGACCAGT TTGGGCAGGC AGCAGGGGTC CTCATTCACA GTATCCGAGA AATAGCTCAG 
TCTCACCGGG ACATGGAGCA GGAACTGGCC CACGCCGTCA ATGCCAGCTC CAAGTCCATG 
GACCGTGTGT ATGGCAAGCC CAGAACCACA GAGGGGCTCA ACTGCAGCTT CGTCCTGGAA 
ATGGTGAATA ACTTCAGAGC TCTGCGCAGT GAGACGGAGC TGCTGCTGTC TGGGAAGATG 
GCCCTGCAGC TGGATCCGCC TCAGAAGGAG CAGCTGGGGA GTGCTCTTGC CGAGATGGAC 
CGACAGCTCA GGAGGCTGGC AGACACCCCG TGGCTCTGCC AGTCCGCAGA GCCCGGCGAC 
GAAGAGAGTG TGATGCTGGA TCTTGCCAAG CGCAGTCGCA GTGGTAAATT CCGCCTCGTG 
ACCAAGTTTA AAAAGGAGAA AAACAACAAG AACAAAGAAG CTCACAGTAG CCTGGGAGCC 
CCGGTTCACC TCTGGGGGAC AGAGGAGGTT GCTGCCTGGC TGGAGCACCT CAGTCTCTGT 
GAGTATAAGG ACATCTTCAC ACGGCACGAC ATCCGGGGCT CTGAGCTCCT GCACCTGGAG 
CGGAGGGACC TCAAGGACCT GGGCGTGACC AAGGTGGGCC ACATGAAGAG GATCCTGTGT 
GGCATCAAGG AGCTGAGCCG CAGCGCCCCC GCCGTCGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression, Purification, and Properties of a Human Arachidonoyl-Specific Isoform of Diacylglycerol Kinase.
Biochemistry56(9)1337-1347(2017 03)
Jennings W,Doshi S,Hota PK,Prodeus A,Black S,Epand RM

A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome.
Skeletal muscle3(1)3(2013 Feb)
Blandin G,Marchand S,Charton K,Dani?le N,Gicquel E,Boucheteil JB,Bentaib A,Barrault L,Stockholm D,Bartoli M,Richard I

Diacylglycerol kinase ? modulates Akt phosphorylation through pleckstrin homology domain leucine-rich repeat protein phosphatase 2 (PHLPP2).
The Journal of biological chemistry288(3)1439-47(2013 Jan)
Crotty TM,Nakano T,Stafforini DM,Topham MK

Meta-analysis of genome-wide association studies identifies six new Loci for serum calcium concentrations.
PLoS genetics9(9)e1003796(2013)
O'Seaghdha CM,Wu H,Yang Q,Kapur K,Guessous I,Zuber AM,K?ttgen A,Stoudmann C,Teumer A,Kutalik Z,Mangino M,Dehghan A,Zhang W,Eiriksdottir G,Li G,Tanaka T,Portas L,Lopez LM,Hayward C,Lohman K,Matsuda K,Padmanabhan S,Firsov D,Sorice R,Ulivi S,Brockhaus AC,Kleber ME,Mahajan A,Ernst FD,Gudnason V,Launer LJ,Mace A,Boerwinckle E,Arking DE,Tanikawa C,Nakamura Y,Brown MJ,Gaspoz JM,Theler JM,Siscovick DS,Psaty BM,Bergmann S,Vollenweider P,Vitart V,Wright AF,Zemunik T,Boban M,Kolcic I,Navarro P,Brown EM,Estrada K,Ding J,Harris TB,Bandinelli S,Hernandez D,Singleton AB,Girotto G,Ruggiero D,d'Adamo AP,Robino A,Meitinger T,Meisinger C,Davies G,Starr JM,Chambers JC,Boehm BO,Winkelmann BR,Huang J,Murgia F,Wild SH,Campbell H,Morris AP,Franco OH,Hofman A,Uitterlinden AG,Rivadeneira F,V?lker U,Hannemann A,Biffar R,Hoffmann W,Shin SY,Lescuyer P,Henry H,Schurmann C,,,Munroe PB,Gasparini P,Pirastu N,Ciullo M,Gieger C,M?rz W,Lind L,Spector TD,Smith AV,Rudan I,Wilson JF,Polasek O,Deary IJ,Pirastu M,Ferrucci L,Liu Y,Kestenbaum B,Kooner JS,Witteman JC,Nauck M,Kao WH,Wallaschofski H,Bonny O,Fox CS,Bochud M

A genome-wide scan of Ashkenazi Jewish Crohn's disease suggests novel susceptibility loci.
PLoS genetics8(3)e1002559(2012)
Kenny EE,Pe'er I,Karban A,Ozelius L,Mitchell AA,Ng SM,Erazo M,Ostrer H,Abraham C,Abreu MT,Atzmon G,Barzilai N,Brant SR,Bressman S,Burns ER,Chowers Y,Clark LN,Darvasi A,Doheny D,Duerr RH,Eliakim R,Giladi N,Gregersen PK,Hakonarson H,Jones MR,Marder K,McGovern DP,Mulle J,Orr-Urtreger A,Proctor DD,Pulver A,Rotter JI,Silverberg MS,Ullman T,Warren ST,Waterman M,Zhang W,Bergman A,Mayer L,Katz S,Desnick RJ,Cho JH,Peter I

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DGKD cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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