LSM10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LSM10
Entrez Gene ID	84967
Full Name	LSM10, U7 small nuclear RNA associated
Synonyms	MST074,MSTP074
General protein information	Preferred Names LSM10, U7 small nuclear RNA associated Names U7 snRNA-associated Sm-like protein LSm10 U7 snRNP-specific Sm-like protein LSM10
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_032881.2	NP_116270.1	U7 snRNA-associated Sm-like protein LSm10
NM_032881.3	NP_116270.1	U7 snRNA-associated Sm-like protein LSm10

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100536270	XP_003200562.1
Pan troglodytes (chimpanzee)	LSM10	XP_513319.2
Canis lupus familiaris (dog)	LSM10	XP_532561.1
Gallus gallus (chicken)	LSM10	XP_417768.1
Danio rerio (zebrafish)	LOC101884591	XP_005173790.1
	LSM10	XP_001110880.1
Rattus norvegicus (Norway rat)	Lsm10	NP_001102446.1
Xenopus tropicalis (tropical clawed frog)	lsm10	NP_001016243.1
Bos taurus (cattle)	LSM10	NP_001077250.1
Homo sapiens (human)	LSM10	NP_116270.1
Mus musculus (house mouse)	Lsm10	NP_001156738.1

Gene Ontology
Bibliography

Related articles in PubMed

U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein.
Skrajna A, Yang XC, Bucholc K, Zhang J, Hall TMT, Dadlez M, Marzluff WF, Dominski Z
RNA (New York, N.Y.)23(6)938-951(2017 06)

The subnuclear organization of histone gene regulatory proteins and 3' end processing factors of normal somatic and embryonic stem cells is compromised in selected human cancer cell types.
Ghule PN, Dominski Z, Lian JB, Stein JL, van Wijnen AJ, Stein GS
Journal of cellular physiology220(1)129-35(2009 Jul)

Purification of human telomerase complexes identifies factors involved in telomerase biogenesis and telomere length regulation.
Fu D, Collins K
Molecular cell28(5)773-85(2007 Dec)

ZFP100, a component of the active U7 snRNP limiting for histone pre-mRNA processing, is required for entry into S phase.
Wagner EJ, Marzluff WF
Molecular and cellular biology26(17)6702-12(2006 Sep)

Toward an assembly line for U7 snRNPs: interactions of U7-specific Lsm proteins with PRMT5 and SMN complexes.
Azzouz TN, Pillai RS, D?pp C, Chari A, Meister G, Kambach C, Fischer U, Sch?mperli D
The Journal of biological chemistry280(41)34435-40(2005 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FLASH plays two roles in 3' end processing of histone pre-mRNAs: It interacts with Lsm11 to form a docking platform for the polyadenylation factors, and it cooperates with SLBP to recruit U7 snRNP to histone pre-mRNA.
Title: U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein.

The subnuclear organization of histone gene regulatory proteins and 3' end processing factors (NPAT/LSM10) of normal somatic and embryonic stem cells is compromised in selected human cancer cell types.
Title: The subnuclear organization of histone gene regulatory proteins and 3' end processing factors of normal somatic and embryonic stem cells is compromised in selected human cancer cell types.

The overexpression of Lsm10 and Lsm11 increases the cellular levels of U7 snRNP but has no effect on histone pre-mRNA processing.
Title: ZFP100, a component of the active U7 snRNP limiting for histone pre-mRNA processing, is required for entry into S phase.

Lsm10 and Lsm11, which replace the Sm proteins D1 and D2 in the histone RNA processing U7 snRNPs, associate with pICln in vitro and in vivo without receiving sDMA modifications and with PRMT5 and SMN complexes
Title: Toward an assembly line for U7 snRNPs: interactions of U7-specific Lsm proteins with PRMT5 and SMN complexes.

Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein.
Title: Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein.

The following LSM10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LSM10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05183	NM_032881.3 Latest version!	Homo sapiens LSM10, U7 small nuclear RNA associated (LSM10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu05183	NM_032881.2	Homo sapiens LSM10, U7 small nuclear RNA associated (LSM10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LSM10 ( NM_032881.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_116270.1 Homo sapiens LSM10, U7 small nuclear RNA associated (LSM10), mRNA.

Price & Availability↑

CloneID	OHu05183
Clone ID Related Accession (Same CDS sequence)	NM_032881.2 , NM_032881.3
Accession Version	NM_032881.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	U7 snRNA-associated Sm-like protein LSm10
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC119675.2, BC007623.1 and AI150247.1. On Jan 31, 2015 this sequence version replaced NM_032881.1. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.250253.1, SRR1803611.394412.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGA GCCATTCAGT GAAGGAGCGG ACCATCTCTG AGAACAGCCT GATCATCCTA 
CTGCAGGGCC TCCAGGGCCG GGTAACCACT GTGGACCTGC GGGATGAGAG CGTGGCCCAC 
GGACGCATAG ACAATGTCGA TGCTTTCATG AACATCCGCC TGGCCAAAGT CACCTACACG 
GACCGTTGGG GGCATCAGGT CAAGCTGGAT GACCTCTTTG TGACAGGCCG CAATGTCCGC 
TACGTCCACA TCCCAGATGA CGTGAACATC ACCTCGACCA TTGAGCAGCA GCTGCAGATT 
ATCCATCGGG TGCGAAACTT TGGTGGCAAG GGCCAAGGCC GGTGGGAATT TCCCCCAAAA 
AACTGTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116270.1
CDS	218..589
Translation	MAVSHSVKERTISENSLIILLQGLQGRVTTVDLRDESVAHGRIDNVDAFMNIRLAKVTYTDRWGHQVKLDDLFVTGRNVRYVHIPDDVNITSTIEQQLQIIHRVRNFGGKGQGRWEFPPKNCK

Target ORF information:

RefSeq Version	NM_032881.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LSM10, U7 small nuclear RNA associated (LSM10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032881.2

ORF Insert Sequence:

ATGGCGGTGA GCCATTCAGT GAAGGAGCGG ACCATCTCTG AGAACAGCCT GATCATCCTA 
CTGCAGGGCC TCCAGGGCCG GGTAACCACT GTGGACCTGC GGGATGAGAG CGTGGCCCAC 
GGACGCATAG ACAATGTCGA TGCTTTCATG AACATCCGCC TGGCCAAAGT CACCTACACG 
GACCGTTGGG GGCATCAGGT CAAGCTGGAT GACCTCTTTG TGACAGGCCG CAATGTCCGC 
TACGTCCACA TCCCAGATGA CGTGAACATC ACCTCGACCA TTGAGCAGCA GCTGCAGATT 
ATCCATCGGG TGCGAAACTT TGGTGGCAAG GGCCAAGGCC GGTGGGAATT TCCCCCAAAA 
AACTGTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LSM10 ( NM_032881.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_116270.1 Homo sapiens LSM10, U7 small nuclear RNA associated (LSM10), mRNA.

Price & Availability↑

CloneID	OHu05183
Clone ID Related Accession (Same CDS sequence)	NM_032881.2 , NM_032881.3
Accession Version	NM_032881.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	U7 snRNA-associated Sm-like protein LSm10
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC119675.2, BC007623.1 and AI150247.1. On Nov 23, 2018 this sequence version replaced NM_032881.2. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1007107.1, SRR1163657.250253.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000315732.3/ ENSP00000319341.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGTGA GCCATTCAGT GAAGGAGCGG ACCATCTCTG AGAACAGCCT GATCATCCTA 
CTGCAGGGCC TCCAGGGCCG GGTAACCACT GTGGACCTGC GGGATGAGAG CGTGGCCCAC 
GGACGCATAG ACAATGTCGA TGCTTTCATG AACATCCGCC TGGCCAAAGT CACCTACACG 
GACCGTTGGG GGCATCAGGT CAAGCTGGAT GACCTCTTTG TGACAGGCCG CAATGTCCGC 
TACGTCCACA TCCCAGATGA CGTGAACATC ACCTCGACCA TTGAGCAGCA GCTGCAGATT 
ATCCATCGGG TGCGAAACTT TGGTGGCAAG GGCCAAGGCC GGTGGGAATT TCCCCCAAAA 
AACTGTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116270.1
CDS	167..538
Translation	MAVSHSVKERTISENSLIILLQGLQGRVTTVDLRDESVAHGRIDNVDAFMNIRLAKVTYTDRWGHQVKLDDLFVTGRNVRYVHIPDDVNITSTIEQQLQIIHRVRNFGGKGQGRWEFPPKNCK

Target ORF information:

RefSeq Version	NM_032881.3
Organism	Homo sapiens(human)
Definition	Homo sapiens LSM10, U7 small nuclear RNA associated (LSM10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032881.3

ORF Insert Sequence:

ATGGCGGTGA GCCATTCAGT GAAGGAGCGG ACCATCTCTG AGAACAGCCT GATCATCCTA 
CTGCAGGGCC TCCAGGGCCG GGTAACCACT GTGGACCTGC GGGATGAGAG CGTGGCCCAC 
GGACGCATAG ACAATGTCGA TGCTTTCATG AACATCCGCC TGGCCAAAGT CACCTACACG 
GACCGTTGGG GGCATCAGGT CAAGCTGGAT GACCTCTTTG TGACAGGCCG CAATGTCCGC 
TACGTCCACA TCCCAGATGA CGTGAACATC ACCTCGACCA TTGAGCAGCA GCTGCAGATT 
ATCCATCGGG TGCGAAACTT TGGTGGCAAG GGCCAAGGCC GGTGGGAATT TCCCCCAAAA 
AACTGTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein.
RNA (New York, N.Y.)23(6)938-951(2017 06)
Skrajna A,Yang XC,Bucholc K,Zhang J,Hall TMT,Dadlez M,Marzluff WF,Dominski Z

The subnuclear organization of histone gene regulatory proteins and 3' end processing factors of normal somatic and embryonic stem cells is compromised in selected human cancer cell types.
Journal of cellular physiology220(1)129-35(2009 Jul)
Ghule PN,Dominski Z,Lian JB,Stein JL,van Wijnen AJ,Stein GS

Purification of human telomerase complexes identifies factors involved in telomerase biogenesis and telomere length regulation.
Molecular cell28(5)773-85(2007 Dec)
Fu D,Collins K

ZFP100, a component of the active U7 snRNP limiting for histone pre-mRNA processing, is required for entry into S phase.
Molecular and cellular biology26(17)6702-12(2006 Sep)
Wagner EJ,Marzluff WF

Toward an assembly line for U7 snRNPs: interactions of U7-specific Lsm proteins with PRMT5 and SMN complexes.
The Journal of biological chemistry280(41)34435-40(2005 Oct)
Azzouz TN,Pillai RS,D?pp C,Chari A,Meister G,Kambach C,Fischer U,Sch?mperli D