POMGNT2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POMGNT2
Entrez Gene ID	84892
Full Name	protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-)
Synonyms	AGO61,C3orf39,GTDC2,MDDGA8
General protein information	Preferred Names protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) Names protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 glycosyltransferase-like domain containing 2 glycosyltransferase-like domain-containing protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p22.1
Summary	This gene encodes a protein with glycosyltransferase activity although its function is not currently known. [provided by RefSeq, Sep 2012].
Disorder MIM:	614828
Disorder Html:	Muscular dystrophy-dystroglycanopathy (congenital with brain and eye anomalies, type A, 8, 614830 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011534164.1	XP_011532466.1	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 isoform X1
XM_005265515.2	XP_005265572.1	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 isoform X1
NM_032806.6	NP_116195.2	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 precursor
XM_005265515.3	XP_005265572.1	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 isoform X1
NM_032806.5	NP_116195.2	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 precursor
XM_011534163.2	XP_011532465.1	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 isoform X1
XM_017007353.1	XP_016862842.1	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	POMGNT2	NP_116195.2
Canis lupus familiaris (dog)	GTDC2	NP_001010957.1
	LOC717032	XP_001108195.1
Mus musculus (house mouse)	Pomgnt2	NP_705768.4
Danio rerio (zebrafish)	pomgnt2	NP_001012384.1
Bos taurus (cattle)	GTDC2	NP_001011682.1
Gallus gallus (chicken)	C2H3ORF39	NP_001012294.1
Pan troglodytes (chimpanzee)	GTDC2	NP_001012742.1
Rattus norvegicus (Norway rat)	Pomgnt2	NP_001009437.1
Xenopus tropicalis (tropical clawed frog)	pomgnt2	NP_001011203.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein
Halmo SM, Singh D, Patel S, Wang S, Edlin M, Boons GJ, Moremen KW, Live D, Wells L
The Journal of biological chemistry292(6)2101-2109(2017 02)

Milder forms of muscular dystrophy associated with POMGNT2 mutations.
Endo Y, Dong M, Noguchi S, Ogawa M, Hayashi YK, Kuru S, Sugiyama K, Nagai S, Ozasa S, Nonaka I, Nishino I
Neurology. Genetics1(4)e33(2015 Dec)

GTDC2 modifies O-mannosylated ?-dystroglycan in the endoplasmic reticulum to generate N-acetyl glucosamine epitopes reactive with CTD110.6 antibody.
Ogawa M, Nakamura N, Nakayama Y, Kurosaka A, Manya H, Kanagawa M, Endo T, Furukawa K, Okajima T
Biochemical and biophysical research communications440(1)88-93(2013 Oct)

SGK196 is a glycosylation-specific O-mannose kinase required for dystroglycan function.
Yoshida-Moriguchi T, Willer T, Anderson ME, Venzke D, Whyte T, Muntoni F, Lee H, Nelson SF, Yu L, Campbell KP
Science (New York, N.Y.)341(6148)896-9(2013 Aug)

Exome sequencing and functional validation in zebrafish identify GTDC2 mutations as a cause of Walker-Warburg syndrome.
Manzini MC, Tambunan DE, Hill RS, Yu TW, Maynard TM, Heinzen EL, Shianna KV, Stevens CR, Partlow JN, Barry BJ, Rodriguez J, Gupta VA, Al-Qudah AK, Eyaid WM, Friedman JM, Salih MA, Clark R, Moroni I, Mora M, Beggs AH, Gabriel SB, Walsh CA
American journal of human genetics91(3)541-7(2012 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

POMGNT2 when two of the conserved amino acids are replaced. These findings begin to define the selectivity of POMGNT2 and suggest that this enzyme functions as a gatekeeper enzyme to prevent the vast majority of O-mannosylated sites on proteins from becoming modified with glycan structures functional for binding laminin globular domain-containing proteins
Title: Protein

GTDC2 generates CTD110.6 antibody-reactive N-acetylglucosamine epitopes on the O-mannosylated alpha-dystroglycan.
Title: GTDC2 modifies O-mannosylated ?-dystroglycan in the endoplasmic reticulum to generate N-acetyl glucosamine epitopes reactive with CTD110.6 antibody.

Using WES in consanguineous WWS-affected families, we found multiple deleterious mutations in GTDC2 (also known as AGO61). .
Title: Exome sequencing and functional validation in zebrafish identify GTDC2 mutations as a cause of Walker-Warburg syndrome.

The following POMGNT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POMGNT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21419	NM_032806.6 Latest version!	Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu21419	XM_017007353.1 Latest version!	Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu21419	XM_011534164.1 Latest version!	Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu21419	XM_011534163.2 Latest version!	Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu21419	XM_005265515.3 Latest version!	Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
View Old Accession Versions >>
OHu21419	XM_005265515.2	Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu21419	NM_032806.5	Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POMGNT2 ( NM_032806.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_116195.2 Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), mRNA.

Price & Availability↑

CloneID	OHu21419
Clone ID Related Accession (Same CDS sequence)	XM_011534164.1 , XM_005265515.2 , NM_032806.6 , XM_005265515.3 , NM_032806.5 , XM_011534163.2 , XM_017007353.1
Accession Version	NM_032806.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-25
Organism	Homo sapiens(human)
Product	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092042.2 and BC060861.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_032806.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.50168.1, BC060861.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000344697.3/ ENSP00000344125.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCACCTCT CGGCGGTGTT CAACGCCCTC CTGGTGTCGG TGCTGGCAGC GGTCCTGTGG 
AAGCATGTGC GGCTGCGTGA GCATGCAGCC ACACTGGAGG AGGAGCTGGC CCTCAGCCGA 
CAGGCCACAG AGCCAGCCCC AGCACTGAGG ATCGACTACC CGAAGGCACT GCAGATCCTG 
ATGGAGGGCG GCACACACAT GGTGTGCACG GGCCGCACGC ACACAGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAACGAGGCT GAGGAGTTCA TCTTCTTCCA TGGCAACACC 
TCTGTCATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CAGCCCTGCT CGACCTATCC 
ACCGTGGAGG ACCACAACAC TCAGTACTTC AACTTCGTGG AGCTGCCTGC TGCTGCCCTG 
CGCTTCATGC CCAAGCCGGT GTTCGTGCCA GACGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGACAACC TCATGCACGT CTTTCATGAC GACCTGCTGC CACTCTTCTA CACCCTGCGG 
CAGTTTCCCG GCCTGGCCCA CGAGGCACGG CTCTTCTTCA TGGAGGGCTG GGGCGAGGGT 
GCACACTTCG ACCTCTACAA GCTGCTCAGC CCCAAGCAGC CTCTCCTGCG GGCACAGCTG 
AAGACCCTGG GCCGGCTGCT GTGCTTCTCC CATGCTTTTG TGGGCCTCTC CAAGATCACT 
ACCTGGTACC AGTATGGCTT TGTGCAGCCC CAGGGCCCGA AGGCCAACAT CCTCGTCTCA 
GGCAATGAGA TCCGGCAGTT TGCACGGTTC ATGACAGAAA AGCTGAACGT GAGCCACACA 
GGAGTCCCCC TAGGCGAGGA GTACATTCTG GTCTTTAGCC GAACCCAGAA CAGACTCATT 
CTGAATGAGG CAGAGCTGCT GCTGGCACTG GCCCAGGAGT TCCAGATGAA GACAGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCTGAT GTCGTGCGGC TGGTCAGCAA TGCCTCCATG 
CTGGTCAGCA TGCATGGGGC CCAGCTGGTC ACCACCCTCT TCCTGCCCCG TGGGGCAACT 
GTGGTAGAGC TCTTCCCATA TGCTGTCAAT CCCGACCACT ACACTCCCTA TAAGACGCTG 
GCCATGCTGC CTGGCATGGA CCTCCAGTAT GTAGCCTGGC GGAACATGAT GCCAGAGAAC 
ACAGTCACAC ACCCTGAGCG GCCCTGGGAT CAGGGGGGCA TCACCCATCT GGACCGGGCT 
GAGCAAGCCC GTATCCTGCA AAGCCGTGAG GTCCCACGGC ATCTCTGTTG CCGGAACCCC 
GAGTGGCTCT TCCGAATCTA CCAGGACACC AAGGTGGACA TCCCGTCCCT CATTCAAACC 
ATACGGCGCG TGGTGAAGGG CCGGCCAGGA CCACGGAAGC AGAAGTGGAC AGTCGGCCTA 
TATCCAGGCA AGGTGCGGGA GGCACGGTGC CAGGCGTCAG TGCATGGCGC CTCCGAGGCC 
CGCCTCACTG TCTCCTGGCA GATCCCATGG AACCTTAAAT ACCTGAAGGT GAGGGAGGTG 
AAGTACGAGG TGTGGCTGCA GGAGCAGGGG GAGAACACCT ACGTGCCTTA CATCCTGGCT 
CTGCAGAACC ACACCTTCAC TGAGAACATC AAGCCCTTCA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAAGAT CCTCCTGGGA CCCTTTGCAG ATGTGCTGGT GTGCAACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116195.2
CDS	350..2092
Translation	MHLSAVFNALLVSVLAAVLWKHVRLREHAATLEEELALSRQATEPAPALRIDYPKALQILMEGGTHMVCTGRTHTDRICRFKWLCYSNEAEEFIFFHGNTSVMLPNLGSRRFQPALLDLSTVEDHNTQYFNFVELPAAALRFMPKPVFVPDVALIANRFNPDNLMHVFHDDLLPLFYTLRQFPGLAHEARLFFMEGWGEGAHFDLYKLLSPKQPLLRAQLKTLGRLLCFSHAFVGLSKITTWYQYGFVQPQGPKANILVSGNEIRQFARFMTEKLNVSHTGVPLGEEYILVFSRTQNRLILNEAELLLALAQEFQMKTVTVSLEDHTFADVVRLVSNASMLVSMHGAQLVTTLFLPRGATVVELFPYAVNPDHYTPYKTLAMLPGMDLQYVAWRNMMPENTVTHPERPWDQGGITHLDRAEQARILQSREVPRHLCCRNPEWLFRIYQDTKVDIPSLIQTIRRVVKGRPGPRKQKWTVGLYPGKVREARCQASVHGASEARLTVSWQIPWNLKYLKVREVKYEVWLQEQGENTYVPYILALQNHTFTENIKPFTTYLVWVRCIFNKILLGPFADVLVCNT

Target ORF information:

RefSeq Version	NM_032806.6
Organism	Homo sapiens(human)
Definition	Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032806.6

ORF Insert Sequence:

ATGCACCTCT CGGCGGTGTT CAACGCCCTC CTGGTGTCGG TGCTGGCAGC GGTCCTGTGG 
AAGCATGTGC GGCTGCGTGA GCATGCAGCC ACACTGGAGG AGGAGCTGGC CCTCAGCCGA 
CAGGCCACAG AGCCAGCCCC AGCACTGAGG ATCGACTACC CGAAGGCACT GCAGATCCTG 
ATGGAGGGCG GCACACACAT GGTGTGCACG GGCCGCACGC ACACAGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAACGAGGCT GAGGAGTTCA TCTTCTTCCA TGGCAACACC 
TCTGTCATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CAGCCCTGCT CGACCTATCC 
ACCGTGGAGG ACCACAACAC TCAGTACTTC AACTTCGTGG AGCTGCCTGC TGCTGCCCTG 
CGCTTCATGC CCAAGCCGGT GTTCGTGCCA GACGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGACAACC TCATGCACGT CTTTCATGAC GACCTGCTGC CACTCTTCTA CACCCTGCGG 
CAGTTTCCCG GCCTGGCCCA CGAGGCACGG CTCTTCTTCA TGGAGGGCTG GGGCGAGGGT 
GCACACTTCG ACCTCTACAA GCTGCTCAGC CCCAAGCAGC CTCTCCTGCG GGCACAGCTG 
AAGACCCTGG GCCGGCTGCT GTGCTTCTCC CATGCTTTTG TGGGCCTCTC CAAGATCACT 
ACCTGGTACC AGTATGGCTT TGTGCAGCCC CAGGGCCCGA AGGCCAACAT CCTCGTCTCA 
GGCAATGAGA TCCGGCAGTT TGCACGGTTC ATGACAGAAA AGCTGAACGT GAGCCACACA 
GGAGTCCCCC TAGGCGAGGA GTACATTCTG GTCTTTAGCC GAACCCAGAA CAGACTCATT 
CTGAATGAGG CAGAGCTGCT GCTGGCACTG GCCCAGGAGT TCCAGATGAA GACAGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCTGAT GTCGTGCGGC TGGTCAGCAA TGCCTCCATG 
CTGGTCAGCA TGCATGGGGC CCAGCTGGTC ACCACCCTCT TCCTGCCCCG TGGGGCAACT 
GTGGTAGAGC TCTTCCCATA TGCTGTCAAT CCCGACCACT ACACTCCCTA TAAGACGCTG 
GCCATGCTGC CTGGCATGGA CCTCCAGTAT GTAGCCTGGC GGAACATGAT GCCAGAGAAC 
ACAGTCACAC ACCCTGAGCG GCCCTGGGAT CAGGGGGGCA TCACCCATCT GGACCGGGCT 
GAGCAAGCCC GTATCCTGCA AAGCCGTGAG GTCCCACGGC ATCTCTGTTG CCGGAACCCC 
GAGTGGCTCT TCCGAATCTA CCAGGACACC AAGGTGGACA TCCCGTCCCT CATTCAAACC 
ATACGGCGCG TGGTGAAGGG CCGGCCAGGA CCACGGAAGC AGAAGTGGAC AGTCGGCCTA 
TATCCAGGCA AGGTGCGGGA GGCACGGTGC CAGGCGTCAG TGCATGGCGC CTCCGAGGCC 
CGCCTCACTG TCTCCTGGCA GATCCCATGG AACCTTAAAT ACCTGAAGGT GAGGGAGGTG 
AAGTACGAGG TGTGGCTGCA GGAGCAGGGG GAGAACACCT ACGTGCCTTA CATCCTGGCT 
CTGCAGAACC ACACCTTCAC TGAGAACATC AAGCCCTTCA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAAGAT CCTCCTGGGA CCCTTTGCAG ATGTGCTGGT GTGCAACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POMGNT2 ( NM_032806.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_116195.2 Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), mRNA.

Price & Availability↑

CloneID	OHu21419
Clone ID Related Accession (Same CDS sequence)	XM_011534164.1 , XM_005265515.2 , NM_032806.6 , XM_005265515.3 , NM_032806.5 , XM_011534163.2 , XM_017007353.1
Accession Version	NM_032806.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092042.2 and BC060861.1. This sequence is a reference standard in the RefSeqGene project. On Sep 11, 2012 this sequence version replaced NM_032806.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: HY010454.1, SRR1803613.268552.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCACCTCT CGGCGGTGTT CAACGCCCTC CTGGTGTCGG TGCTGGCAGC GGTCCTGTGG 
AAGCATGTGC GGCTGCGTGA GCATGCAGCC ACACTGGAGG AGGAGCTGGC CCTCAGCCGA 
CAGGCCACAG AGCCAGCCCC AGCACTGAGG ATCGACTACC CGAAGGCACT GCAGATCCTG 
ATGGAGGGCG GCACACACAT GGTGTGCACG GGCCGCACGC ACACAGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAACGAGGCT GAGGAGTTCA TCTTCTTCCA TGGCAACACC 
TCTGTCATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CAGCCCTGCT CGACCTATCC 
ACCGTGGAGG ACCACAACAC TCAGTACTTC AACTTCGTGG AGCTGCCTGC TGCTGCCCTG 
CGCTTCATGC CCAAGCCGGT GTTCGTGCCA GACGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGACAACC TCATGCACGT CTTTCATGAC GACCTGCTGC CACTCTTCTA CACCCTGCGG 
CAGTTTCCCG GCCTGGCCCA CGAGGCACGG CTCTTCTTCA TGGAGGGCTG GGGCGAGGGT 
GCACACTTCG ACCTCTACAA GCTGCTCAGC CCCAAGCAGC CTCTCCTGCG GGCACAGCTG 
AAGACCCTGG GCCGGCTGCT GTGCTTCTCC CATGCTTTTG TGGGCCTCTC CAAGATCACT 
ACCTGGTACC AGTATGGCTT TGTGCAGCCC CAGGGCCCGA AGGCCAACAT CCTCGTCTCA 
GGCAATGAGA TCCGGCAGTT TGCACGGTTC ATGACAGAAA AGCTGAACGT GAGCCACACA 
GGAGTCCCCC TAGGCGAGGA GTACATTCTG GTCTTTAGCC GAACCCAGAA CAGACTCATT 
CTGAATGAGG CAGAGCTGCT GCTGGCACTG GCCCAGGAGT TCCAGATGAA GACAGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCTGAT GTCGTGCGGC TGGTCAGCAA TGCCTCCATG 
CTGGTCAGCA TGCATGGGGC CCAGCTGGTC ACCACCCTCT TCCTGCCCCG TGGGGCAACT 
GTGGTAGAGC TCTTCCCATA TGCTGTCAAT CCCGACCACT ACACTCCCTA TAAGACGCTG 
GCCATGCTGC CTGGCATGGA CCTCCAGTAT GTAGCCTGGC GGAACATGAT GCCAGAGAAC 
ACAGTCACAC ACCCTGAGCG GCCCTGGGAT CAGGGGGGCA TCACCCATCT GGACCGGGCT 
GAGCAAGCCC GTATCCTGCA AAGCCGTGAG GTCCCACGGC ATCTCTGTTG CCGGAACCCC 
GAGTGGCTCT TCCGAATCTA CCAGGACACC AAGGTGGACA TCCCGTCCCT CATTCAAACC 
ATACGGCGCG TGGTGAAGGG CCGGCCAGGA CCACGGAAGC AGAAGTGGAC AGTCGGCCTA 
TATCCAGGCA AGGTGCGGGA GGCACGGTGC CAGGCGTCAG TGCATGGCGC CTCCGAGGCC 
CGCCTCACTG TCTCCTGGCA GATCCCATGG AACCTTAAAT ACCTGAAGGT GAGGGAGGTG 
AAGTACGAGG TGTGGCTGCA GGAGCAGGGG GAGAACACCT ACGTGCCTTA CATCCTGGCT 
CTGCAGAACC ACACCTTCAC TGAGAACATC AAGCCCTTCA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAAGAT CCTCCTGGGA CCCTTTGCAG ATGTGCTGGT GTGCAACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116195.2
CDS	354..2096
Translation	MHLSAVFNALLVSVLAAVLWKHVRLREHAATLEEELALSRQATEPAPALRIDYPKALQILMEGGTHMVCTGRTHTDRICRFKWLCYSNEAEEFIFFHGNTSVMLPNLGSRRFQPALLDLSTVEDHNTQYFNFVELPAAALRFMPKPVFVPDVALIANRFNPDNLMHVFHDDLLPLFYTLRQFPGLAHEARLFFMEGWGEGAHFDLYKLLSPKQPLLRAQLKTLGRLLCFSHAFVGLSKITTWYQYGFVQPQGPKANILVSGNEIRQFARFMTEKLNVSHTGVPLGEEYILVFSRTQNRLILNEAELLLALAQEFQMKTVTVSLEDHTFADVVRLVSNASMLVSMHGAQLVTTLFLPRGATVVELFPYAVNPDHYTPYKTLAMLPGMDLQYVAWRNMMPENTVTHPERPWDQGGITHLDRAEQARILQSREVPRHLCCRNPEWLFRIYQDTKVDIPSLIQTIRRVVKGRPGPRKQKWTVGLYPGKVREARCQASVHGASEARLTVSWQIPWNLKYLKVREVKYEVWLQEQGENTYVPYILALQNHTFTENIKPFTTYLVWVRCIFNKILLGPFADVLVCNT

Target ORF information:

RefSeq Version	NM_032806.5
Organism	Homo sapiens(human)
Definition	Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032806.5

ORF Insert Sequence:

ATGCACCTCT CGGCGGTGTT CAACGCCCTC CTGGTGTCGG TGCTGGCAGC GGTCCTGTGG 
AAGCATGTGC GGCTGCGTGA GCATGCAGCC ACACTGGAGG AGGAGCTGGC CCTCAGCCGA 
CAGGCCACAG AGCCAGCCCC AGCACTGAGG ATCGACTACC CGAAGGCACT GCAGATCCTG 
ATGGAGGGCG GCACACACAT GGTGTGCACG GGCCGCACGC ACACAGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAACGAGGCT GAGGAGTTCA TCTTCTTCCA TGGCAACACC 
TCTGTCATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CAGCCCTGCT CGACCTATCC 
ACCGTGGAGG ACCACAACAC TCAGTACTTC AACTTCGTGG AGCTGCCTGC TGCTGCCCTG 
CGCTTCATGC CCAAGCCGGT GTTCGTGCCA GACGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGACAACC TCATGCACGT CTTTCATGAC GACCTGCTGC CACTCTTCTA CACCCTGCGG 
CAGTTTCCCG GCCTGGCCCA CGAGGCACGG CTCTTCTTCA TGGAGGGCTG GGGCGAGGGT 
GCACACTTCG ACCTCTACAA GCTGCTCAGC CCCAAGCAGC CTCTCCTGCG GGCACAGCTG 
AAGACCCTGG GCCGGCTGCT GTGCTTCTCC CATGCTTTTG TGGGCCTCTC CAAGATCACT 
ACCTGGTACC AGTATGGCTT TGTGCAGCCC CAGGGCCCGA AGGCCAACAT CCTCGTCTCA 
GGCAATGAGA TCCGGCAGTT TGCACGGTTC ATGACAGAAA AGCTGAACGT GAGCCACACA 
GGAGTCCCCC TAGGCGAGGA GTACATTCTG GTCTTTAGCC GAACCCAGAA CAGACTCATT 
CTGAATGAGG CAGAGCTGCT GCTGGCACTG GCCCAGGAGT TCCAGATGAA GACAGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCTGAT GTCGTGCGGC TGGTCAGCAA TGCCTCCATG 
CTGGTCAGCA TGCATGGGGC CCAGCTGGTC ACCACCCTCT TCCTGCCCCG TGGGGCAACT 
GTGGTAGAGC TCTTCCCATA TGCTGTCAAT CCCGACCACT ACACTCCCTA TAAGACGCTG 
GCCATGCTGC CTGGCATGGA CCTCCAGTAT GTAGCCTGGC GGAACATGAT GCCAGAGAAC 
ACAGTCACAC ACCCTGAGCG GCCCTGGGAT CAGGGGGGCA TCACCCATCT GGACCGGGCT 
GAGCAAGCCC GTATCCTGCA AAGCCGTGAG GTCCCACGGC ATCTCTGTTG CCGGAACCCC 
GAGTGGCTCT TCCGAATCTA CCAGGACACC AAGGTGGACA TCCCGTCCCT CATTCAAACC 
ATACGGCGCG TGGTGAAGGG CCGGCCAGGA CCACGGAAGC AGAAGTGGAC AGTCGGCCTA 
TATCCAGGCA AGGTGCGGGA GGCACGGTGC CAGGCGTCAG TGCATGGCGC CTCCGAGGCC 
CGCCTCACTG TCTCCTGGCA GATCCCATGG AACCTTAAAT ACCTGAAGGT GAGGGAGGTG 
AAGTACGAGG TGTGGCTGCA GGAGCAGGGG GAGAACACCT ACGTGCCTTA CATCCTGGCT 
CTGCAGAACC ACACCTTCAC TGAGAACATC AAGCCCTTCA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAAGAT CCTCCTGGGA CCCTTTGCAG ATGTGCTGGT GTGCAACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POMGNT2 ( XM_011534164.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532466.1 Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu21419
Clone ID Related Accession (Same CDS sequence)	XM_011534164.1 , XM_005265515.2 , NM_032806.6 , XM_005265515.3 , NM_032806.5 , XM_011534163.2 , XM_017007353.1
Accession Version	XM_011534164.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCTCT CGGCGGTGTT CAACGCCCTC CTGGTGTCGG TGCTGGCAGC GGTCCTGTGG 
AAGCATGTGC GGCTGCGTGA GCATGCAGCC ACACTGGAGG AGGAGCTGGC CCTCAGCCGA 
CAGGCCACAG AGCCAGCCCC AGCACTGAGG ATCGACTACC CGAAGGCACT GCAGATCCTG 
ATGGAGGGCG GCACACACAT GGTGTGCACG GGCCGCACGC ACACAGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAACGAGGCT GAGGAGTTCA TCTTCTTCCA TGGCAACACC 
TCTGTCATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CAGCCCTGCT CGACCTATCC 
ACCGTGGAGG ACCACAACAC TCAGTACTTC AACTTCGTGG AGCTGCCTGC TGCTGCCCTG 
CGCTTCATGC CCAAGCCGGT GTTCGTGCCA GACGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGACAACC TCATGCACGT CTTTCATGAC GACCTGCTGC CACTCTTCTA CACCCTGCGG 
CAGTTTCCCG GCCTGGCCCA CGAGGCACGG CTCTTCTTCA TGGAGGGCTG GGGCGAGGGT 
GCACACTTCG ACCTCTACAA GCTGCTCAGC CCCAAGCAGC CTCTCCTGCG GGCACAGCTG 
AAGACCCTGG GCCGGCTGCT GTGCTTCTCC CATGCTTTTG TGGGCCTCTC CAAGATCACT 
ACCTGGTACC AGTATGGCTT TGTGCAGCCC CAGGGCCCGA AGGCCAACAT CCTCGTCTCA 
GGCAATGAGA TCCGGCAGTT TGCACGGTTC ATGACAGAAA AGCTGAACGT GAGCCACACA 
GGAGTCCCCC TAGGCGAGGA GTACATTCTG GTCTTTAGCC GAACCCAGAA CAGACTCATT 
CTGAATGAGG CAGAGCTGCT GCTGGCACTG GCCCAGGAGT TCCAGATGAA GACAGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCTGAT GTCGTGCGGC TGGTCAGCAA TGCCTCCATG 
CTGGTCAGCA TGCATGGGGC CCAGCTGGTC ACCACCCTCT TCCTGCCCCG TGGGGCAACT 
GTGGTAGAGC TCTTCCCATA TGCTGTCAAT CCCGACCACT ACACTCCCTA TAAGACGCTG 
GCCATGCTGC CTGGCATGGA CCTCCAGTAT GTAGCCTGGC GGAACATGAT GCCAGAGAAC 
ACAGTCACAC ACCCTGAGCG GCCCTGGGAT CAGGGGGGCA TCACCCATCT GGACCGGGCT 
GAGCAAGCCC GTATCCTGCA AAGCCGTGAG GTCCCACGGC ATCTCTGTTG CCGGAACCCC 
GAGTGGCTCT TCCGAATCTA CCAGGACACC AAGGTGGACA TCCCGTCCCT CATTCAAACC 
ATACGGCGCG TGGTGAAGGG CCGGCCAGGA CCACGGAAGC AGAAGTGGAC AGTCGGCCTA 
TATCCAGGCA AGGTGCGGGA GGCACGGTGC CAGGCGTCAG TGCATGGCGC CTCCGAGGCC 
CGCCTCACTG TCTCCTGGCA GATCCCATGG AACCTTAAAT ACCTGAAGGT GAGGGAGGTG 
AAGTACGAGG TGTGGCTGCA GGAGCAGGGG GAGAACACCT ACGTGCCTTA CATCCTGGCT 
CTGCAGAACC ACACCTTCAC TGAGAACATC AAGCCCTTCA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAAGAT CCTCCTGGGA CCCTTTGCAG ATGTGCTGGT GTGCAACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532466.1
CDS	1055..2797
Translation	MHLSAVFNALLVSVLAAVLWKHVRLREHAATLEEELALSRQATEPAPALRIDYPKALQILMEGGTHMVCTGRTHTDRICRFKWLCYSNEAEEFIFFHGNTSVMLPNLGSRRFQPALLDLSTVEDHNTQYFNFVELPAAALRFMPKPVFVPDVALIANRFNPDNLMHVFHDDLLPLFYTLRQFPGLAHEARLFFMEGWGEGAHFDLYKLLSPKQPLLRAQLKTLGRLLCFSHAFVGLSKITTWYQYGFVQPQGPKANILVSGNEIRQFARFMTEKLNVSHTGVPLGEEYILVFSRTQNRLILNEAELLLALAQEFQMKTVTVSLEDHTFADVVRLVSNASMLVSMHGAQLVTTLFLPRGATVVELFPYAVNPDHYTPYKTLAMLPGMDLQYVAWRNMMPENTVTHPERPWDQGGITHLDRAEQARILQSREVPRHLCCRNPEWLFRIYQDTKVDIPSLIQTIRRVVKGRPGPRKQKWTVGLYPGKVREARCQASVHGASEARLTVSWQIPWNLKYLKVREVKYEVWLQEQGENTYVPYILALQNHTFTENIKPFTTYLVWVRCIFNKILLGPFADVLVCNT

Target ORF information:

RefSeq Version	XM_011534164.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534164.1

ORF Insert Sequence:

ATGCACCTCT CGGCGGTGTT CAACGCCCTC CTGGTGTCGG TGCTGGCAGC GGTCCTGTGG 
AAGCATGTGC GGCTGCGTGA GCATGCAGCC ACACTGGAGG AGGAGCTGGC CCTCAGCCGA 
CAGGCCACAG AGCCAGCCCC AGCACTGAGG ATCGACTACC CGAAGGCACT GCAGATCCTG 
ATGGAGGGCG GCACACACAT GGTGTGCACG GGCCGCACGC ACACAGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAACGAGGCT GAGGAGTTCA TCTTCTTCCA TGGCAACACC 
TCTGTCATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CAGCCCTGCT CGACCTATCC 
ACCGTGGAGG ACCACAACAC TCAGTACTTC AACTTCGTGG AGCTGCCTGC TGCTGCCCTG 
CGCTTCATGC CCAAGCCGGT GTTCGTGCCA GACGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGACAACC TCATGCACGT CTTTCATGAC GACCTGCTGC CACTCTTCTA CACCCTGCGG 
CAGTTTCCCG GCCTGGCCCA CGAGGCACGG CTCTTCTTCA TGGAGGGCTG GGGCGAGGGT 
GCACACTTCG ACCTCTACAA GCTGCTCAGC CCCAAGCAGC CTCTCCTGCG GGCACAGCTG 
AAGACCCTGG GCCGGCTGCT GTGCTTCTCC CATGCTTTTG TGGGCCTCTC CAAGATCACT 
ACCTGGTACC AGTATGGCTT TGTGCAGCCC CAGGGCCCGA AGGCCAACAT CCTCGTCTCA 
GGCAATGAGA TCCGGCAGTT TGCACGGTTC ATGACAGAAA AGCTGAACGT GAGCCACACA 
GGAGTCCCCC TAGGCGAGGA GTACATTCTG GTCTTTAGCC GAACCCAGAA CAGACTCATT 
CTGAATGAGG CAGAGCTGCT GCTGGCACTG GCCCAGGAGT TCCAGATGAA GACAGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCTGAT GTCGTGCGGC TGGTCAGCAA TGCCTCCATG 
CTGGTCAGCA TGCATGGGGC CCAGCTGGTC ACCACCCTCT TCCTGCCCCG TGGGGCAACT 
GTGGTAGAGC TCTTCCCATA TGCTGTCAAT CCCGACCACT ACACTCCCTA TAAGACGCTG 
GCCATGCTGC CTGGCATGGA CCTCCAGTAT GTAGCCTGGC GGAACATGAT GCCAGAGAAC 
ACAGTCACAC ACCCTGAGCG GCCCTGGGAT CAGGGGGGCA TCACCCATCT GGACCGGGCT 
GAGCAAGCCC GTATCCTGCA AAGCCGTGAG GTCCCACGGC ATCTCTGTTG CCGGAACCCC 
GAGTGGCTCT TCCGAATCTA CCAGGACACC AAGGTGGACA TCCCGTCCCT CATTCAAACC 
ATACGGCGCG TGGTGAAGGG CCGGCCAGGA CCACGGAAGC AGAAGTGGAC AGTCGGCCTA 
TATCCAGGCA AGGTGCGGGA GGCACGGTGC CAGGCGTCAG TGCATGGCGC CTCCGAGGCC 
CGCCTCACTG TCTCCTGGCA GATCCCATGG AACCTTAAAT ACCTGAAGGT GAGGGAGGTG 
AAGTACGAGG TGTGGCTGCA GGAGCAGGGG GAGAACACCT ACGTGCCTTA CATCCTGGCT 
CTGCAGAACC ACACCTTCAC TGAGAACATC AAGCCCTTCA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAAGAT CCTCCTGGGA CCCTTTGCAG ATGTGCTGGT GTGCAACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POMGNT2 ( XM_005265515.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005265572.1 Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu21419
Clone ID Related Accession (Same CDS sequence)	XM_011534164.1 , XM_005265515.2 , NM_032806.6 , XM_005265515.3 , NM_032806.5 , XM_011534163.2 , XM_017007353.1
Accession Version	XM_005265515.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005265515.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCTCT CGGCGGTGTT CAACGCCCTC CTGGTGTCGG TGCTGGCAGC GGTCCTGTGG 
AAGCATGTGC GGCTGCGTGA GCATGCAGCC ACACTGGAGG AGGAGCTGGC CCTCAGCCGA 
CAGGCCACAG AGCCAGCCCC AGCACTGAGG ATCGACTACC CGAAGGCACT GCAGATCCTG 
ATGGAGGGCG GCACACACAT GGTGTGCACG GGCCGCACGC ACACAGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAACGAGGCT GAGGAGTTCA TCTTCTTCCA TGGCAACACC 
TCTGTCATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CAGCCCTGCT CGACCTATCC 
ACCGTGGAGG ACCACAACAC TCAGTACTTC AACTTCGTGG AGCTGCCTGC TGCTGCCCTG 
CGCTTCATGC CCAAGCCGGT GTTCGTGCCA GACGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGACAACC TCATGCACGT CTTTCATGAC GACCTGCTGC CACTCTTCTA CACCCTGCGG 
CAGTTTCCCG GCCTGGCCCA CGAGGCACGG CTCTTCTTCA TGGAGGGCTG GGGCGAGGGT 
GCACACTTCG ACCTCTACAA GCTGCTCAGC CCCAAGCAGC CTCTCCTGCG GGCACAGCTG 
AAGACCCTGG GCCGGCTGCT GTGCTTCTCC CATGCTTTTG TGGGCCTCTC CAAGATCACT 
ACCTGGTACC AGTATGGCTT TGTGCAGCCC CAGGGCCCGA AGGCCAACAT CCTCGTCTCA 
GGCAATGAGA TCCGGCAGTT TGCACGGTTC ATGACAGAAA AGCTGAACGT GAGCCACACA 
GGAGTCCCCC TAGGCGAGGA GTACATTCTG GTCTTTAGCC GAACCCAGAA CAGACTCATT 
CTGAATGAGG CAGAGCTGCT GCTGGCACTG GCCCAGGAGT TCCAGATGAA GACAGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCTGAT GTCGTGCGGC TGGTCAGCAA TGCCTCCATG 
CTGGTCAGCA TGCATGGGGC CCAGCTGGTC ACCACCCTCT TCCTGCCCCG TGGGGCAACT 
GTGGTAGAGC TCTTCCCATA TGCTGTCAAT CCCGACCACT ACACTCCCTA TAAGACGCTG 
GCCATGCTGC CTGGCATGGA CCTCCAGTAT GTAGCCTGGC GGAACATGAT GCCAGAGAAC 
ACAGTCACAC ACCCTGAGCG GCCCTGGGAT CAGGGGGGCA TCACCCATCT GGACCGGGCT 
GAGCAAGCCC GTATCCTGCA AAGCCGTGAG GTCCCACGGC ATCTCTGTTG CCGGAACCCC 
GAGTGGCTCT TCCGAATCTA CCAGGACACC AAGGTGGACA TCCCGTCCCT CATTCAAACC 
ATACGGCGCG TGGTGAAGGG CCGGCCAGGA CCACGGAAGC AGAAGTGGAC AGTCGGCCTA 
TATCCAGGCA AGGTGCGGGA GGCACGGTGC CAGGCGTCAG TGCATGGCGC CTCCGAGGCC 
CGCCTCACTG TCTCCTGGCA GATCCCATGG AACCTTAAAT ACCTGAAGGT GAGGGAGGTG 
AAGTACGAGG TGTGGCTGCA GGAGCAGGGG GAGAACACCT ACGTGCCTTA CATCCTGGCT 
CTGCAGAACC ACACCTTCAC TGAGAACATC AAGCCCTTCA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAAGAT CCTCCTGGGA CCCTTTGCAG ATGTGCTGGT GTGCAACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005265572.1
CDS	485..2227
Translation	MHLSAVFNALLVSVLAAVLWKHVRLREHAATLEEELALSRQATEPAPALRIDYPKALQILMEGGTHMVCTGRTHTDRICRFKWLCYSNEAEEFIFFHGNTSVMLPNLGSRRFQPALLDLSTVEDHNTQYFNFVELPAAALRFMPKPVFVPDVALIANRFNPDNLMHVFHDDLLPLFYTLRQFPGLAHEARLFFMEGWGEGAHFDLYKLLSPKQPLLRAQLKTLGRLLCFSHAFVGLSKITTWYQYGFVQPQGPKANILVSGNEIRQFARFMTEKLNVSHTGVPLGEEYILVFSRTQNRLILNEAELLLALAQEFQMKTVTVSLEDHTFADVVRLVSNASMLVSMHGAQLVTTLFLPRGATVVELFPYAVNPDHYTPYKTLAMLPGMDLQYVAWRNMMPENTVTHPERPWDQGGITHLDRAEQARILQSREVPRHLCCRNPEWLFRIYQDTKVDIPSLIQTIRRVVKGRPGPRKQKWTVGLYPGKVREARCQASVHGASEARLTVSWQIPWNLKYLKVREVKYEVWLQEQGENTYVPYILALQNHTFTENIKPFTTYLVWVRCIFNKILLGPFADVLVCNT

Target ORF information:

RefSeq Version	XM_005265515.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005265515.2

ORF Insert Sequence:

ATGCACCTCT CGGCGGTGTT CAACGCCCTC CTGGTGTCGG TGCTGGCAGC GGTCCTGTGG 
AAGCATGTGC GGCTGCGTGA GCATGCAGCC ACACTGGAGG AGGAGCTGGC CCTCAGCCGA 
CAGGCCACAG AGCCAGCCCC AGCACTGAGG ATCGACTACC CGAAGGCACT GCAGATCCTG 
ATGGAGGGCG GCACACACAT GGTGTGCACG GGCCGCACGC ACACAGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAACGAGGCT GAGGAGTTCA TCTTCTTCCA TGGCAACACC 
TCTGTCATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CAGCCCTGCT CGACCTATCC 
ACCGTGGAGG ACCACAACAC TCAGTACTTC AACTTCGTGG AGCTGCCTGC TGCTGCCCTG 
CGCTTCATGC CCAAGCCGGT GTTCGTGCCA GACGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGACAACC TCATGCACGT CTTTCATGAC GACCTGCTGC CACTCTTCTA CACCCTGCGG 
CAGTTTCCCG GCCTGGCCCA CGAGGCACGG CTCTTCTTCA TGGAGGGCTG GGGCGAGGGT 
GCACACTTCG ACCTCTACAA GCTGCTCAGC CCCAAGCAGC CTCTCCTGCG GGCACAGCTG 
AAGACCCTGG GCCGGCTGCT GTGCTTCTCC CATGCTTTTG TGGGCCTCTC CAAGATCACT 
ACCTGGTACC AGTATGGCTT TGTGCAGCCC CAGGGCCCGA AGGCCAACAT CCTCGTCTCA 
GGCAATGAGA TCCGGCAGTT TGCACGGTTC ATGACAGAAA AGCTGAACGT GAGCCACACA 
GGAGTCCCCC TAGGCGAGGA GTACATTCTG GTCTTTAGCC GAACCCAGAA CAGACTCATT 
CTGAATGAGG CAGAGCTGCT GCTGGCACTG GCCCAGGAGT TCCAGATGAA GACAGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCTGAT GTCGTGCGGC TGGTCAGCAA TGCCTCCATG 
CTGGTCAGCA TGCATGGGGC CCAGCTGGTC ACCACCCTCT TCCTGCCCCG TGGGGCAACT 
GTGGTAGAGC TCTTCCCATA TGCTGTCAAT CCCGACCACT ACACTCCCTA TAAGACGCTG 
GCCATGCTGC CTGGCATGGA CCTCCAGTAT GTAGCCTGGC GGAACATGAT GCCAGAGAAC 
ACAGTCACAC ACCCTGAGCG GCCCTGGGAT CAGGGGGGCA TCACCCATCT GGACCGGGCT 
GAGCAAGCCC GTATCCTGCA AAGCCGTGAG GTCCCACGGC ATCTCTGTTG CCGGAACCCC 
GAGTGGCTCT TCCGAATCTA CCAGGACACC AAGGTGGACA TCCCGTCCCT CATTCAAACC 
ATACGGCGCG TGGTGAAGGG CCGGCCAGGA CCACGGAAGC AGAAGTGGAC AGTCGGCCTA 
TATCCAGGCA AGGTGCGGGA GGCACGGTGC CAGGCGTCAG TGCATGGCGC CTCCGAGGCC 
CGCCTCACTG TCTCCTGGCA GATCCCATGG AACCTTAAAT ACCTGAAGGT GAGGGAGGTG 
AAGTACGAGG TGTGGCTGCA GGAGCAGGGG GAGAACACCT ACGTGCCTTA CATCCTGGCT 
CTGCAGAACC ACACCTTCAC TGAGAACATC AAGCCCTTCA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAAGAT CCTCCTGGGA CCCTTTGCAG ATGTGCTGGT GTGCAACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POMGNT2 ( XM_005265515.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005265572.1 Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu21419
Clone ID Related Accession (Same CDS sequence)	XM_011534164.1 , XM_005265515.2 , NM_032806.6 , XM_005265515.3 , NM_032806.5 , XM_011534163.2 , XM_017007353.1
Accession Version	XM_005265515.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005265515.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCTCT CGGCGGTGTT CAACGCCCTC CTGGTGTCGG TGCTGGCAGC GGTCCTGTGG 
AAGCATGTGC GGCTGCGTGA GCATGCAGCC ACACTGGAGG AGGAGCTGGC CCTCAGCCGA 
CAGGCCACAG AGCCAGCCCC AGCACTGAGG ATCGACTACC CGAAGGCACT GCAGATCCTG 
ATGGAGGGCG GCACACACAT GGTGTGCACG GGCCGCACGC ACACAGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAACGAGGCT GAGGAGTTCA TCTTCTTCCA TGGCAACACC 
TCTGTCATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CAGCCCTGCT CGACCTATCC 
ACCGTGGAGG ACCACAACAC TCAGTACTTC AACTTCGTGG AGCTGCCTGC TGCTGCCCTG 
CGCTTCATGC CCAAGCCGGT GTTCGTGCCA GACGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGACAACC TCATGCACGT CTTTCATGAC GACCTGCTGC CACTCTTCTA CACCCTGCGG 
CAGTTTCCCG GCCTGGCCCA CGAGGCACGG CTCTTCTTCA TGGAGGGCTG GGGCGAGGGT 
GCACACTTCG ACCTCTACAA GCTGCTCAGC CCCAAGCAGC CTCTCCTGCG GGCACAGCTG 
AAGACCCTGG GCCGGCTGCT GTGCTTCTCC CATGCTTTTG TGGGCCTCTC CAAGATCACT 
ACCTGGTACC AGTATGGCTT TGTGCAGCCC CAGGGCCCGA AGGCCAACAT CCTCGTCTCA 
GGCAATGAGA TCCGGCAGTT TGCACGGTTC ATGACAGAAA AGCTGAACGT GAGCCACACA 
GGAGTCCCCC TAGGCGAGGA GTACATTCTG GTCTTTAGCC GAACCCAGAA CAGACTCATT 
CTGAATGAGG CAGAGCTGCT GCTGGCACTG GCCCAGGAGT TCCAGATGAA GACAGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCTGAT GTCGTGCGGC TGGTCAGCAA TGCCTCCATG 
CTGGTCAGCA TGCATGGGGC CCAGCTGGTC ACCACCCTCT TCCTGCCCCG TGGGGCAACT 
GTGGTAGAGC TCTTCCCATA TGCTGTCAAT CCCGACCACT ACACTCCCTA TAAGACGCTG 
GCCATGCTGC CTGGCATGGA CCTCCAGTAT GTAGCCTGGC GGAACATGAT GCCAGAGAAC 
ACAGTCACAC ACCCTGAGCG GCCCTGGGAT CAGGGGGGCA TCACCCATCT GGACCGGGCT 
GAGCAAGCCC GTATCCTGCA AAGCCGTGAG GTCCCACGGC ATCTCTGTTG CCGGAACCCC 
GAGTGGCTCT TCCGAATCTA CCAGGACACC AAGGTGGACA TCCCGTCCCT CATTCAAACC 
ATACGGCGCG TGGTGAAGGG CCGGCCAGGA CCACGGAAGC AGAAGTGGAC AGTCGGCCTA 
TATCCAGGCA AGGTGCGGGA GGCACGGTGC CAGGCGTCAG TGCATGGCGC CTCCGAGGCC 
CGCCTCACTG TCTCCTGGCA GATCCCATGG AACCTTAAAT ACCTGAAGGT GAGGGAGGTG 
AAGTACGAGG TGTGGCTGCA GGAGCAGGGG GAGAACACCT ACGTGCCTTA CATCCTGGCT 
CTGCAGAACC ACACCTTCAC TGAGAACATC AAGCCCTTCA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAAGAT CCTCCTGGGA CCCTTTGCAG ATGTGCTGGT GTGCAACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005265572.1
CDS	332..2074
Translation	MHLSAVFNALLVSVLAAVLWKHVRLREHAATLEEELALSRQATEPAPALRIDYPKALQILMEGGTHMVCTGRTHTDRICRFKWLCYSNEAEEFIFFHGNTSVMLPNLGSRRFQPALLDLSTVEDHNTQYFNFVELPAAALRFMPKPVFVPDVALIANRFNPDNLMHVFHDDLLPLFYTLRQFPGLAHEARLFFMEGWGEGAHFDLYKLLSPKQPLLRAQLKTLGRLLCFSHAFVGLSKITTWYQYGFVQPQGPKANILVSGNEIRQFARFMTEKLNVSHTGVPLGEEYILVFSRTQNRLILNEAELLLALAQEFQMKTVTVSLEDHTFADVVRLVSNASMLVSMHGAQLVTTLFLPRGATVVELFPYAVNPDHYTPYKTLAMLPGMDLQYVAWRNMMPENTVTHPERPWDQGGITHLDRAEQARILQSREVPRHLCCRNPEWLFRIYQDTKVDIPSLIQTIRRVVKGRPGPRKQKWTVGLYPGKVREARCQASVHGASEARLTVSWQIPWNLKYLKVREVKYEVWLQEQGENTYVPYILALQNHTFTENIKPFTTYLVWVRCIFNKILLGPFADVLVCNT

Target ORF information:

RefSeq Version	XM_005265515.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005265515.3

ORF Insert Sequence:

ATGCACCTCT CGGCGGTGTT CAACGCCCTC CTGGTGTCGG TGCTGGCAGC GGTCCTGTGG 
AAGCATGTGC GGCTGCGTGA GCATGCAGCC ACACTGGAGG AGGAGCTGGC CCTCAGCCGA 
CAGGCCACAG AGCCAGCCCC AGCACTGAGG ATCGACTACC CGAAGGCACT GCAGATCCTG 
ATGGAGGGCG GCACACACAT GGTGTGCACG GGCCGCACGC ACACAGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAACGAGGCT GAGGAGTTCA TCTTCTTCCA TGGCAACACC 
TCTGTCATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CAGCCCTGCT CGACCTATCC 
ACCGTGGAGG ACCACAACAC TCAGTACTTC AACTTCGTGG AGCTGCCTGC TGCTGCCCTG 
CGCTTCATGC CCAAGCCGGT GTTCGTGCCA GACGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGACAACC TCATGCACGT CTTTCATGAC GACCTGCTGC CACTCTTCTA CACCCTGCGG 
CAGTTTCCCG GCCTGGCCCA CGAGGCACGG CTCTTCTTCA TGGAGGGCTG GGGCGAGGGT 
GCACACTTCG ACCTCTACAA GCTGCTCAGC CCCAAGCAGC CTCTCCTGCG GGCACAGCTG 
AAGACCCTGG GCCGGCTGCT GTGCTTCTCC CATGCTTTTG TGGGCCTCTC CAAGATCACT 
ACCTGGTACC AGTATGGCTT TGTGCAGCCC CAGGGCCCGA AGGCCAACAT CCTCGTCTCA 
GGCAATGAGA TCCGGCAGTT TGCACGGTTC ATGACAGAAA AGCTGAACGT GAGCCACACA 
GGAGTCCCCC TAGGCGAGGA GTACATTCTG GTCTTTAGCC GAACCCAGAA CAGACTCATT 
CTGAATGAGG CAGAGCTGCT GCTGGCACTG GCCCAGGAGT TCCAGATGAA GACAGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCTGAT GTCGTGCGGC TGGTCAGCAA TGCCTCCATG 
CTGGTCAGCA TGCATGGGGC CCAGCTGGTC ACCACCCTCT TCCTGCCCCG TGGGGCAACT 
GTGGTAGAGC TCTTCCCATA TGCTGTCAAT CCCGACCACT ACACTCCCTA TAAGACGCTG 
GCCATGCTGC CTGGCATGGA CCTCCAGTAT GTAGCCTGGC GGAACATGAT GCCAGAGAAC 
ACAGTCACAC ACCCTGAGCG GCCCTGGGAT CAGGGGGGCA TCACCCATCT GGACCGGGCT 
GAGCAAGCCC GTATCCTGCA AAGCCGTGAG GTCCCACGGC ATCTCTGTTG CCGGAACCCC 
GAGTGGCTCT TCCGAATCTA CCAGGACACC AAGGTGGACA TCCCGTCCCT CATTCAAACC 
ATACGGCGCG TGGTGAAGGG CCGGCCAGGA CCACGGAAGC AGAAGTGGAC AGTCGGCCTA 
TATCCAGGCA AGGTGCGGGA GGCACGGTGC CAGGCGTCAG TGCATGGCGC CTCCGAGGCC 
CGCCTCACTG TCTCCTGGCA GATCCCATGG AACCTTAAAT ACCTGAAGGT GAGGGAGGTG 
AAGTACGAGG TGTGGCTGCA GGAGCAGGGG GAGAACACCT ACGTGCCTTA CATCCTGGCT 
CTGCAGAACC ACACCTTCAC TGAGAACATC AAGCCCTTCA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAAGAT CCTCCTGGGA CCCTTTGCAG ATGTGCTGGT GTGCAACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POMGNT2 ( XM_011534163.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532465.1 Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X2, mRNA.

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CloneID	OHu21419
Clone ID Related Accession (Same CDS sequence)	XM_011534164.1 , XM_005265515.2 , NM_032806.6 , XM_005265515.3 , NM_032806.5 , XM_011534163.2 , XM_017007353.1
Accession Version	XM_011534163.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCTCT CGGCGGTGTT CAACGCCCTC CTGGTGTCGG TGCTGGCAGC GGTCCTGTGG 
AAGCATGTGC GGCTGCGTGA GCATGCAGCC ACACTGGAGG AGGAGCTGGC CCTCAGCCGA 
CAGGCCACAG AGCCAGCCCC AGCACTGAGG ATCGACTACC CGAAGGCACT GCAGATCCTG 
ATGGAGGGCG GCACACACAT GGTGTGCACG GGCCGCACGC ACACAGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAACGAGGCT GAGGAGTTCA TCTTCTTCCA TGGCAACACC 
TCTGTCATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CAGCCCTGCT CGACCTATCC 
ACCGTGGAGG ACCACAACAC TCAGTACTTC AACTTCGTGG AGCTGCCTGC TGCTGCCCTG 
CGCTTCATGC CCAAGCCGGT GTTCGTGCCA GACGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGACAACC TCATGCACGT CTTTCATGAC GACCTGCTGC CACTCTTCTA CACCCTGCGG 
CAGTTTCCCG GCCTGGCCCA CGAGGCACGG CTCTTCTTCA TGGAGGGCTG GGGCGAGGGT 
GCACACTTCG ACCTCTACAA GCTGCTCAGC CCCAAGCAGC CTCTCCTGCG GGCACAGCTG 
AAGACCCTGG GCCGGCTGCT GTGCTTCTCC CATGCTTTTG TGGGCCTCTC CAAGATCACT 
ACCTGGTACC AGTATGGCTT TGTGCAGCCC CAGGGCCCGA AGGCCAACAT CCTCGTCTCA 
GGCAATGAGA TCCGGCAGTT TGCACGGTTC ATGACAGAAA AGCTGAACGT GAGCCACACA 
GGAGTCCCCC TAGGCGAGGA GTACATTCTG GTCTTTAGCC GAACCCAGAA CAGACTCATT 
CTGAATGAGG CAGAGCTGCT GCTGGCACTG GCCCAGGAGT TCCAGATGAA GACAGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCTGAT GTCGTGCGGC TGGTCAGCAA TGCCTCCATG 
CTGGTCAGCA TGCATGGGGC CCAGCTGGTC ACCACCCTCT TCCTGCCCCG TGGGGCAACT 
GTGGTAGAGC TCTTCCCATA TGCTGTCAAT CCCGACCACT ACACTCCCTA TAAGACGCTG 
GCCATGCTGC CTGGCATGGA CCTCCAGTAT GTAGCCTGGC GGAACATGAT GCCAGAGAAC 
ACAGTCACAC ACCCTGAGCG GCCCTGGGAT CAGGGGGGCA TCACCCATCT GGACCGGGCT 
GAGCAAGCCC GTATCCTGCA AAGCCGTGAG GTCCCACGGC ATCTCTGTTG CCGGAACCCC 
GAGTGGCTCT TCCGAATCTA CCAGGACACC AAGGTGGACA TCCCGTCCCT CATTCAAACC 
ATACGGCGCG TGGTGAAGGG CCGGCCAGGA CCACGGAAGC AGAAGTGGAC AGTCGGCCTA 
TATCCAGGCA AGGTGCGGGA GGCACGGTGC CAGGCGTCAG TGCATGGCGC CTCCGAGGCC 
CGCCTCACTG TCTCCTGGCA GATCCCATGG AACCTTAAAT ACCTGAAGGT GAGGGAGGTG 
AAGTACGAGG TGTGGCTGCA GGAGCAGGGG GAGAACACCT ACGTGCCTTA CATCCTGGCT 
CTGCAGAACC ACACCTTCAC TGAGAACATC AAGCCCTTCA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAAGAT CCTCCTGGGA CCCTTTGCAG ATGTGCTGGT GTGCAACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532465.1
CDS	473..2215
Translation	MHLSAVFNALLVSVLAAVLWKHVRLREHAATLEEELALSRQATEPAPALRIDYPKALQILMEGGTHMVCTGRTHTDRICRFKWLCYSNEAEEFIFFHGNTSVMLPNLGSRRFQPALLDLSTVEDHNTQYFNFVELPAAALRFMPKPVFVPDVALIANRFNPDNLMHVFHDDLLPLFYTLRQFPGLAHEARLFFMEGWGEGAHFDLYKLLSPKQPLLRAQLKTLGRLLCFSHAFVGLSKITTWYQYGFVQPQGPKANILVSGNEIRQFARFMTEKLNVSHTGVPLGEEYILVFSRTQNRLILNEAELLLALAQEFQMKTVTVSLEDHTFADVVRLVSNASMLVSMHGAQLVTTLFLPRGATVVELFPYAVNPDHYTPYKTLAMLPGMDLQYVAWRNMMPENTVTHPERPWDQGGITHLDRAEQARILQSREVPRHLCCRNPEWLFRIYQDTKVDIPSLIQTIRRVVKGRPGPRKQKWTVGLYPGKVREARCQASVHGASEARLTVSWQIPWNLKYLKVREVKYEVWLQEQGENTYVPYILALQNHTFTENIKPFTTYLVWVRCIFNKILLGPFADVLVCNT

Target ORF information:

RefSeq Version	XM_011534163.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534163.2

ORF Insert Sequence:

ATGCACCTCT CGGCGGTGTT CAACGCCCTC CTGGTGTCGG TGCTGGCAGC GGTCCTGTGG 
AAGCATGTGC GGCTGCGTGA GCATGCAGCC ACACTGGAGG AGGAGCTGGC CCTCAGCCGA 
CAGGCCACAG AGCCAGCCCC AGCACTGAGG ATCGACTACC CGAAGGCACT GCAGATCCTG 
ATGGAGGGCG GCACACACAT GGTGTGCACG GGCCGCACGC ACACAGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAACGAGGCT GAGGAGTTCA TCTTCTTCCA TGGCAACACC 
TCTGTCATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CAGCCCTGCT CGACCTATCC 
ACCGTGGAGG ACCACAACAC TCAGTACTTC AACTTCGTGG AGCTGCCTGC TGCTGCCCTG 
CGCTTCATGC CCAAGCCGGT GTTCGTGCCA GACGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGACAACC TCATGCACGT CTTTCATGAC GACCTGCTGC CACTCTTCTA CACCCTGCGG 
CAGTTTCCCG GCCTGGCCCA CGAGGCACGG CTCTTCTTCA TGGAGGGCTG GGGCGAGGGT 
GCACACTTCG ACCTCTACAA GCTGCTCAGC CCCAAGCAGC CTCTCCTGCG GGCACAGCTG 
AAGACCCTGG GCCGGCTGCT GTGCTTCTCC CATGCTTTTG TGGGCCTCTC CAAGATCACT 
ACCTGGTACC AGTATGGCTT TGTGCAGCCC CAGGGCCCGA AGGCCAACAT CCTCGTCTCA 
GGCAATGAGA TCCGGCAGTT TGCACGGTTC ATGACAGAAA AGCTGAACGT GAGCCACACA 
GGAGTCCCCC TAGGCGAGGA GTACATTCTG GTCTTTAGCC GAACCCAGAA CAGACTCATT 
CTGAATGAGG CAGAGCTGCT GCTGGCACTG GCCCAGGAGT TCCAGATGAA GACAGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCTGAT GTCGTGCGGC TGGTCAGCAA TGCCTCCATG 
CTGGTCAGCA TGCATGGGGC CCAGCTGGTC ACCACCCTCT TCCTGCCCCG TGGGGCAACT 
GTGGTAGAGC TCTTCCCATA TGCTGTCAAT CCCGACCACT ACACTCCCTA TAAGACGCTG 
GCCATGCTGC CTGGCATGGA CCTCCAGTAT GTAGCCTGGC GGAACATGAT GCCAGAGAAC 
ACAGTCACAC ACCCTGAGCG GCCCTGGGAT CAGGGGGGCA TCACCCATCT GGACCGGGCT 
GAGCAAGCCC GTATCCTGCA AAGCCGTGAG GTCCCACGGC ATCTCTGTTG CCGGAACCCC 
GAGTGGCTCT TCCGAATCTA CCAGGACACC AAGGTGGACA TCCCGTCCCT CATTCAAACC 
ATACGGCGCG TGGTGAAGGG CCGGCCAGGA CCACGGAAGC AGAAGTGGAC AGTCGGCCTA 
TATCCAGGCA AGGTGCGGGA GGCACGGTGC CAGGCGTCAG TGCATGGCGC CTCCGAGGCC 
CGCCTCACTG TCTCCTGGCA GATCCCATGG AACCTTAAAT ACCTGAAGGT GAGGGAGGTG 
AAGTACGAGG TGTGGCTGCA GGAGCAGGGG GAGAACACCT ACGTGCCTTA CATCCTGGCT 
CTGCAGAACC ACACCTTCAC TGAGAACATC AAGCCCTTCA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAAGAT CCTCCTGGGA CCCTTTGCAG ATGTGCTGGT GTGCAACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POMGNT2 ( XM_017007353.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862842.1 Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu21419
Clone ID Related Accession (Same CDS sequence)	XM_011534164.1 , XM_005265515.2 , NM_032806.6 , XM_005265515.3 , NM_032806.5 , XM_011534163.2 , XM_017007353.1
Accession Version	XM_017007353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCTCT CGGCGGTGTT CAACGCCCTC CTGGTGTCGG TGCTGGCAGC GGTCCTGTGG 
AAGCATGTGC GGCTGCGTGA GCATGCAGCC ACACTGGAGG AGGAGCTGGC CCTCAGCCGA 
CAGGCCACAG AGCCAGCCCC AGCACTGAGG ATCGACTACC CGAAGGCACT GCAGATCCTG 
ATGGAGGGCG GCACACACAT GGTGTGCACG GGCCGCACGC ACACAGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAACGAGGCT GAGGAGTTCA TCTTCTTCCA TGGCAACACC 
TCTGTCATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CAGCCCTGCT CGACCTATCC 
ACCGTGGAGG ACCACAACAC TCAGTACTTC AACTTCGTGG AGCTGCCTGC TGCTGCCCTG 
CGCTTCATGC CCAAGCCGGT GTTCGTGCCA GACGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGACAACC TCATGCACGT CTTTCATGAC GACCTGCTGC CACTCTTCTA CACCCTGCGG 
CAGTTTCCCG GCCTGGCCCA CGAGGCACGG CTCTTCTTCA TGGAGGGCTG GGGCGAGGGT 
GCACACTTCG ACCTCTACAA GCTGCTCAGC CCCAAGCAGC CTCTCCTGCG GGCACAGCTG 
AAGACCCTGG GCCGGCTGCT GTGCTTCTCC CATGCTTTTG TGGGCCTCTC CAAGATCACT 
ACCTGGTACC AGTATGGCTT TGTGCAGCCC CAGGGCCCGA AGGCCAACAT CCTCGTCTCA 
GGCAATGAGA TCCGGCAGTT TGCACGGTTC ATGACAGAAA AGCTGAACGT GAGCCACACA 
GGAGTCCCCC TAGGCGAGGA GTACATTCTG GTCTTTAGCC GAACCCAGAA CAGACTCATT 
CTGAATGAGG CAGAGCTGCT GCTGGCACTG GCCCAGGAGT TCCAGATGAA GACAGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCTGAT GTCGTGCGGC TGGTCAGCAA TGCCTCCATG 
CTGGTCAGCA TGCATGGGGC CCAGCTGGTC ACCACCCTCT TCCTGCCCCG TGGGGCAACT 
GTGGTAGAGC TCTTCCCATA TGCTGTCAAT CCCGACCACT ACACTCCCTA TAAGACGCTG 
GCCATGCTGC CTGGCATGGA CCTCCAGTAT GTAGCCTGGC GGAACATGAT GCCAGAGAAC 
ACAGTCACAC ACCCTGAGCG GCCCTGGGAT CAGGGGGGCA TCACCCATCT GGACCGGGCT 
GAGCAAGCCC GTATCCTGCA AAGCCGTGAG GTCCCACGGC ATCTCTGTTG CCGGAACCCC 
GAGTGGCTCT TCCGAATCTA CCAGGACACC AAGGTGGACA TCCCGTCCCT CATTCAAACC 
ATACGGCGCG TGGTGAAGGG CCGGCCAGGA CCACGGAAGC AGAAGTGGAC AGTCGGCCTA 
TATCCAGGCA AGGTGCGGGA GGCACGGTGC CAGGCGTCAG TGCATGGCGC CTCCGAGGCC 
CGCCTCACTG TCTCCTGGCA GATCCCATGG AACCTTAAAT ACCTGAAGGT GAGGGAGGTG 
AAGTACGAGG TGTGGCTGCA GGAGCAGGGG GAGAACACCT ACGTGCCTTA CATCCTGGCT 
CTGCAGAACC ACACCTTCAC TGAGAACATC AAGCCCTTCA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAAGAT CCTCCTGGGA CCCTTTGCAG ATGTGCTGGT GTGCAACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862842.1
CDS	564..2306
Translation	MHLSAVFNALLVSVLAAVLWKHVRLREHAATLEEELALSRQATEPAPALRIDYPKALQILMEGGTHMVCTGRTHTDRICRFKWLCYSNEAEEFIFFHGNTSVMLPNLGSRRFQPALLDLSTVEDHNTQYFNFVELPAAALRFMPKPVFVPDVALIANRFNPDNLMHVFHDDLLPLFYTLRQFPGLAHEARLFFMEGWGEGAHFDLYKLLSPKQPLLRAQLKTLGRLLCFSHAFVGLSKITTWYQYGFVQPQGPKANILVSGNEIRQFARFMTEKLNVSHTGVPLGEEYILVFSRTQNRLILNEAELLLALAQEFQMKTVTVSLEDHTFADVVRLVSNASMLVSMHGAQLVTTLFLPRGATVVELFPYAVNPDHYTPYKTLAMLPGMDLQYVAWRNMMPENTVTHPERPWDQGGITHLDRAEQARILQSREVPRHLCCRNPEWLFRIYQDTKVDIPSLIQTIRRVVKGRPGPRKQKWTVGLYPGKVREARCQASVHGASEARLTVSWQIPWNLKYLKVREVKYEVWLQEQGENTYVPYILALQNHTFTENIKPFTTYLVWVRCIFNKILLGPFADVLVCNT

Target ORF information:

RefSeq Version	XM_017007353.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (POMGNT2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007353.1

ORF Insert Sequence:

ATGCACCTCT CGGCGGTGTT CAACGCCCTC CTGGTGTCGG TGCTGGCAGC GGTCCTGTGG 
AAGCATGTGC GGCTGCGTGA GCATGCAGCC ACACTGGAGG AGGAGCTGGC CCTCAGCCGA 
CAGGCCACAG AGCCAGCCCC AGCACTGAGG ATCGACTACC CGAAGGCACT GCAGATCCTG 
ATGGAGGGCG GCACACACAT GGTGTGCACG GGCCGCACGC ACACAGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAACGAGGCT GAGGAGTTCA TCTTCTTCCA TGGCAACACC 
TCTGTCATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CAGCCCTGCT CGACCTATCC 
ACCGTGGAGG ACCACAACAC TCAGTACTTC AACTTCGTGG AGCTGCCTGC TGCTGCCCTG 
CGCTTCATGC CCAAGCCGGT GTTCGTGCCA GACGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGACAACC TCATGCACGT CTTTCATGAC GACCTGCTGC CACTCTTCTA CACCCTGCGG 
CAGTTTCCCG GCCTGGCCCA CGAGGCACGG CTCTTCTTCA TGGAGGGCTG GGGCGAGGGT 
GCACACTTCG ACCTCTACAA GCTGCTCAGC CCCAAGCAGC CTCTCCTGCG GGCACAGCTG 
AAGACCCTGG GCCGGCTGCT GTGCTTCTCC CATGCTTTTG TGGGCCTCTC CAAGATCACT 
ACCTGGTACC AGTATGGCTT TGTGCAGCCC CAGGGCCCGA AGGCCAACAT CCTCGTCTCA 
GGCAATGAGA TCCGGCAGTT TGCACGGTTC ATGACAGAAA AGCTGAACGT GAGCCACACA 
GGAGTCCCCC TAGGCGAGGA GTACATTCTG GTCTTTAGCC GAACCCAGAA CAGACTCATT 
CTGAATGAGG CAGAGCTGCT GCTGGCACTG GCCCAGGAGT TCCAGATGAA GACAGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCTGAT GTCGTGCGGC TGGTCAGCAA TGCCTCCATG 
CTGGTCAGCA TGCATGGGGC CCAGCTGGTC ACCACCCTCT TCCTGCCCCG TGGGGCAACT 
GTGGTAGAGC TCTTCCCATA TGCTGTCAAT CCCGACCACT ACACTCCCTA TAAGACGCTG 
GCCATGCTGC CTGGCATGGA CCTCCAGTAT GTAGCCTGGC GGAACATGAT GCCAGAGAAC 
ACAGTCACAC ACCCTGAGCG GCCCTGGGAT CAGGGGGGCA TCACCCATCT GGACCGGGCT 
GAGCAAGCCC GTATCCTGCA AAGCCGTGAG GTCCCACGGC ATCTCTGTTG CCGGAACCCC 
GAGTGGCTCT TCCGAATCTA CCAGGACACC AAGGTGGACA TCCCGTCCCT CATTCAAACC 
ATACGGCGCG TGGTGAAGGG CCGGCCAGGA CCACGGAAGC AGAAGTGGAC AGTCGGCCTA 
TATCCAGGCA AGGTGCGGGA GGCACGGTGC CAGGCGTCAG TGCATGGCGC CTCCGAGGCC 
CGCCTCACTG TCTCCTGGCA GATCCCATGG AACCTTAAAT ACCTGAAGGT GAGGGAGGTG 
AAGTACGAGG TGTGGCTGCA GGAGCAGGGG GAGAACACCT ACGTGCCTTA CATCCTGGCT 
CTGCAGAACC ACACCTTCAC TGAGAACATC AAGCCCTTCA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAAGAT CCTCCTGGGA CCCTTTGCAG ATGTGCTGGT GTGCAACACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein
The Journal of biological chemistry292(6)2101-2109(2017 02)
Halmo SM,Singh D,Patel S,Wang S,Edlin M,Boons GJ,Moremen KW,Live D,Wells L

Milder forms of muscular dystrophy associated with POMGNT2 mutations.
Neurology. Genetics1(4)e33(2015 Dec)
Endo Y,Dong M,Noguchi S,Ogawa M,Hayashi YK,Kuru S,Sugiyama K,Nagai S,Ozasa S,Nonaka I,Nishino I

GTDC2 modifies O-mannosylated ?-dystroglycan in the endoplasmic reticulum to generate N-acetyl glucosamine epitopes reactive with CTD110.6 antibody.
Biochemical and biophysical research communications440(1)88-93(2013 Oct)
Ogawa M,Nakamura N,Nakayama Y,Kurosaka A,Manya H,Kanagawa M,Endo T,Furukawa K,Okajima T

SGK196 is a glycosylation-specific O-mannose kinase required for dystroglycan function.
Science (New York, N.Y.)341(6148)896-9(2013 Aug)
Yoshida-Moriguchi T,Willer T,Anderson ME,Venzke D,Whyte T,Muntoni F,Lee H,Nelson SF,Yu L,Campbell KP

Exome sequencing and functional validation in zebrafish identify GTDC2 mutations as a cause of Walker-Warburg syndrome.
American journal of human genetics91(3)541-7(2012 Sep)
Manzini MC,Tambunan DE,Hill RS,Yu TW,Maynard TM,Heinzen EL,Shianna KV,Stevens CR,Partlow JN,Barry BJ,Rodriguez J,Gupta VA,Al-Qudah AK,Eyaid WM,Friedman JM,Salih MA,Clark R,Moroni I,Mora M,Beggs AH,Gabriel SB,Walsh CA

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POMGNT2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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