RPUSD4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPUSD4
Entrez Gene ID	84881
Full Name	RNA pseudouridylate synthase domain containing 4
General protein information	Preferred Names RNA pseudouridylate synthase domain containing 4 Names mitochondrial RNA pseudouridine synthase RPUSD4 RNA pseudouridylate synthase domain-containing protein 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q24.2
Disorder MIM:	617488

mRNA and Protein(s)

mRNA	Protein	Name
NM_001363516.1	NP_001350445.1	mitochondrial RNA pseudouridine synthase RPUSD4 isoform c
NM_001363516.2	NP_001350445.1	mitochondrial RNA pseudouridine synthase RPUSD4 isoform c
NM_032795.2	NP_116184.2	mitochondrial RNA pseudouridine synthase RPUSD4 isoform a
NM_032795.3	NP_116184.2	mitochondrial RNA pseudouridine synthase RPUSD4 isoform a
NM_001144827.1	NP_001138299.1	mitochondrial RNA pseudouridine synthase RPUSD4 isoform b
XM_011543039.2	XP_011541341.1	mitochondrial RNA pseudouridine synthase RPUSD4 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RPUSD4	NP_116184.2
Gallus gallus (chicken)	RPUSD4	XP_001231937.2
Danio rerio (zebrafish)	rpusd4	NP_001003495.1
Xenopus tropicalis (tropical clawed frog)	LOC734064	NP_001039205.1
	RPUSD4	XP_001112402.1
Canis lupus familiaris (dog)	RPUSD4	XP_005619636.1
Mus musculus (house mouse)	Rpusd4	NP_082316.1
Pan troglodytes (chimpanzee)	RPUSD4	XP_508851.2
Bos taurus (cattle)	RPUSD4	NP_001015616.1
Rattus norvegicus (Norway rat)	Rpusd4	NP_001020455.1

Gene Ontology
Bibliography

Related articles in PubMed

The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules.
Zaganelli S, Rebelo-Guiomar P, Maundrell K, Rozanska A, Pierredon S, Powell CA, Jourdain AA, Hulo N, Lightowlers RN, Chrzanowska-Lightowlers ZM, Minczuk M, Martinou JC
The Journal of biological chemistry292(11)4519-4532(2017 03)

A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.
Arroyo JD, Jourdain AA, Calvo SE, Ballarano CA, Doench JG, Root DE, Mootha VK
Cell metabolism24(6)875-885(2016 Dec)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RPUSD4 binds 16S mt-rRNA, mt-tRNA(Met), and mt-tRNA(Phe), and is responsible for pseudouridylation of the latter.
Title: The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following RPUSD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPUSD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05104	NM_032795.3 Latest version!	Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu73805	XM_011543039.2 Latest version!	Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu02254	NM_001144827.1 Latest version!	Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu106868	NM_001363516.2 Latest version!	Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu106868	NM_001363516.1	Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu05104	NM_032795.2	Homo sapiens RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPUSD4 ( NM_032795.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_116184.2 Homo sapiens RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05104
Clone ID Related Accession (Same CDS sequence)	NM_032795.2 , NM_032795.3
Accession Version	NM_032795.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Homo sapiens(human)
Product	mitochondrial RNA pseudouridine synthase RPUSD4 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB051034.1, BC014131.2, DB542764.1 and BU679173.1. On Jan 24, 2009 this sequence version replaced NM_032795.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1803613.270668.1, SRR1660805.107055.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGC CCAGGTGGAG CGCGTCGGGC CCCTGGATCC GGGGAAACGG CCAAGGTTGC 
GGGAGTCTCT TCACTCTCGT CTCAAAGCCA TTTTGTGCCG CTGCCGCTGC CTCTACGGCC 
ATAAATGCCC AGAGATTAGC GGAGAAGCTC CGAGCCCAGA AACGGGAACA AGACACAAAG 
AAGGAGCCGG TGTCCACAAA CGCTGTTCAG CGGAGAGTGC AAGAAATAGT GCGGTTCACA 
CGGCAGCTGC AGCGAGTCCA CCCCAACGTG CTTGCTAAGG CACTGACCCG AGGAATTCTC 
CACCAGGACA AGAACCTTGT GGTCATCAAT AAGCCCTACG GTCTCCCTGT GCATGGTGGC 
CCTGGGGTCC AGCTCTGCAT CACTGATGTA CTACCTATCC TGGCAAAGAT GCTTCATGGC 
CACAAGGCAG AGCCCTTGCA TCTGTGCCAC CGGCTGGACA AGGAAACCAC AGGTGTAATG 
GTGTTGGCTT GGGACAAGGA CATGGCACAT CAAGTCCAAG AGTTGTTTAG AACCCGTCAG 
GTGGTGAAGA AGTACTGGGC CATCACTGTG CATGTCCCCA TGCCCTCAGC AGGAGTCGTG 
GACATCCCCA TTGTGGAGAA GGAGGCGCAA GGCCAGCAGC AACACCACAA GATGACATTG 
TCCCCGAGCT ACCGCATGGA CGATGGGAAA ATGGTGAAAG TGCGGCGCAG CCGGAATGCG 
CAAGTTGCTG TAACTCAGTA CCAGGTGCTC AGCAGCACTC TCTCCTCCGC CCTCGTGGAG 
CTCCAGCCCA TCACTGGAAT AAAACATCAG CTTCGAGTTC ACTTGTCTTT TGGATTGGAT 
TGTCCAATCC TTGGTGATCA CAAGTACTCA GACTGGAATA GGTTGGCCCC CCAGAAGCTG 
TCTGTGGGCA CCCTGAAGAA GCTGGGGCTA GAACAGTCGA AGGCCCGCTA CATCCCCCTT 
CACCTGCACG CCCGGCAGCT GATCCTGCCT GCCCTGGGGT CCGGGAAGGA GGAACTCAAC 
TTGGTCTGCA AACTTCCTCG CTTCTTTGTG CATTCCCTGC ACCGCCTGCG TTTAGAGATG 
CCAAATGAGG ATCAAAATGA GAACAATGAA GCCAAGTGTC TGGGAGCACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116184.2
CDS	55..1188
Translation	MAAPRWSASGPWIRGNGQGCGSLFTLVSKPFCAAAAASTAINAQRLAEKLRAQKREQDTKKEPVSTNAVQRRVQEIVRFTRQLQRVHPNVLAKALTRGILHQDKNLVVINKPYGLPVHGGPGVQLCITDVLPILAKMLHGHKAEPLHLCHRLDKETTGVMVLAWDKDMAHQVQELFRTRQVVKKYWAITVHVPMPSAGVVDIPIVEKEAQGQQQHHKMTLSPSYRMDDGKMVKVRRSRNAQVAVTQYQVLSSTLSSALVELQPITGIKHQLRVHLSFGLDCPILGDHKYSDWNRLAPQKLSVGTLKKLGLEQSKARYIPLHLHARQLILPALGSGKEELNLVCKLPRFFVHSLHRLRLEMPNEDQNENNEAKCLGAQ

Target ORF information:

RefSeq Version	NM_032795.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032795.2

ORF Insert Sequence:

ATGGCGGCGC CCAGGTGGAG CGCGTCGGGC CCCTGGATCC GGGGAAACGG CCAAGGTTGC 
GGGAGTCTCT TCACTCTCGT CTCAAAGCCA TTTTGTGCCG CTGCCGCTGC CTCTACGGCC 
ATAAATGCCC AGAGATTAGC GGAGAAGCTC CGAGCCCAGA AACGGGAACA AGACACAAAG 
AAGGAGCCGG TGTCCACAAA CGCTGTTCAG CGGAGAGTGC AAGAAATAGT GCGGTTCACA 
CGGCAGCTGC AGCGAGTCCA CCCCAACGTG CTTGCTAAGG CACTGACCCG AGGAATTCTC 
CACCAGGACA AGAACCTTGT GGTCATCAAT AAGCCCTACG GTCTCCCTGT GCATGGTGGC 
CCTGGGGTCC AGCTCTGCAT CACTGATGTA CTACCTATCC TGGCAAAGAT GCTTCATGGC 
CACAAGGCAG AGCCCTTGCA TCTGTGCCAC CGGCTGGACA AGGAAACCAC AGGTGTAATG 
GTGTTGGCTT GGGACAAGGA CATGGCACAT CAAGTCCAAG AGTTGTTTAG AACCCGTCAG 
GTGGTGAAGA AGTACTGGGC CATCACTGTG CATGTCCCCA TGCCCTCAGC AGGAGTCGTG 
GACATCCCCA TTGTGGAGAA GGAGGCGCAA GGCCAGCAGC AACACCACAA GATGACATTG 
TCCCCGAGCT ACCGCATGGA CGATGGGAAA ATGGTGAAAG TGCGGCGCAG CCGGAATGCG 
CAAGTTGCTG TAACTCAGTA CCAGGTGCTC AGCAGCACTC TCTCCTCCGC CCTCGTGGAG 
CTCCAGCCCA TCACTGGAAT AAAACATCAG CTTCGAGTTC ACTTGTCTTT TGGATTGGAT 
TGTCCAATCC TTGGTGATCA CAAGTACTCA GACTGGAATA GGTTGGCCCC CCAGAAGCTG 
TCTGTGGGCA CCCTGAAGAA GCTGGGGCTA GAACAGTCGA AGGCCCGCTA CATCCCCCTT 
CACCTGCACG CCCGGCAGCT GATCCTGCCT GCCCTGGGGT CCGGGAAGGA GGAACTCAAC 
TTGGTCTGCA AACTTCCTCG CTTCTTTGTG CATTCCCTGC ACCGCCTGCG TTTAGAGATG 
CCAAATGAGG ATCAAAATGA GAACAATGAA GCCAAGTGTC TGGGAGCACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPUSD4 ( NM_032795.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_116184.2 Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05104
Clone ID Related Accession (Same CDS sequence)	NM_032795.2 , NM_032795.3
Accession Version	NM_032795.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	mitochondrial RNA pseudouridine synthase RPUSD4 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC014131.2, DB542764.1 and BU679173.1. On Nov 22, 2018 this sequence version replaced NM_032795.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1803617.208019.1, SRR1660805.107055.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000298317.9/ ENSP00000298317.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGC CCAGGTGGAG CGCGTCGGGC CCCTGGATCC GGGGAAACGG CCAAGGTTGC 
GGGAGTCTCT TCACTCTCGT CTCAAAGCCA TTTTGTGCCG CTGCCGCTGC CTCTACGGCC 
ATAAATGCCC AGAGATTAGC GGAGAAGCTC CGAGCCCAGA AACGGGAACA AGACACAAAG 
AAGGAGCCGG TGTCCACAAA CGCTGTTCAG CGGAGAGTGC AAGAAATAGT GCGGTTCACA 
CGGCAGCTGC AGCGAGTCCA CCCCAACGTG CTTGCTAAGG CACTGACCCG AGGAATTCTC 
CACCAGGACA AGAACCTTGT GGTCATCAAT AAGCCCTACG GTCTCCCTGT GCATGGTGGC 
CCTGGGGTCC AGCTCTGCAT CACTGATGTA CTACCTATCC TGGCAAAGAT GCTTCATGGC 
CACAAGGCAG AGCCCTTGCA TCTGTGCCAC CGGCTGGACA AGGAAACCAC AGGTGTAATG 
GTGTTGGCTT GGGACAAGGA CATGGCACAT CAAGTCCAAG AGTTGTTTAG AACCCGTCAG 
GTGGTGAAGA AGTACTGGGC CATCACTGTG CATGTCCCCA TGCCCTCAGC AGGAGTCGTG 
GACATCCCCA TTGTGGAGAA GGAGGCGCAA GGCCAGCAGC AACACCACAA GATGACATTG 
TCCCCGAGCT ACCGCATGGA CGATGGGAAA ATGGTGAAAG TGCGGCGCAG CCGGAATGCG 
CAAGTTGCTG TAACTCAGTA CCAGGTGCTC AGCAGCACTC TCTCCTCCGC CCTCGTGGAG 
CTCCAGCCCA TCACTGGAAT AAAACATCAG CTTCGAGTTC ACTTGTCTTT TGGATTGGAT 
TGTCCAATCC TTGGTGATCA CAAGTACTCA GACTGGAATA GGTTGGCCCC CCAGAAGCTG 
TCTGTGGGCA CCCTGAAGAA GCTGGGGCTA GAACAGTCGA AGGCCCGCTA CATCCCCCTT 
CACCTGCACG CCCGGCAGCT GATCCTGCCT GCCCTGGGGT CCGGGAAGGA GGAACTCAAC 
TTGGTCTGCA AACTTCCTCG CTTCTTTGTG CATTCCCTGC ACCGCCTGCG TTTAGAGATG 
CCAAATGAGG ATCAAAATGA GAACAATGAA GCCAAGTGTC TGGGAGCACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116184.2
CDS	13..1146
Translation	MAAPRWSASGPWIRGNGQGCGSLFTLVSKPFCAAAAASTAINAQRLAEKLRAQKREQDTKKEPVSTNAVQRRVQEIVRFTRQLQRVHPNVLAKALTRGILHQDKNLVVINKPYGLPVHGGPGVQLCITDVLPILAKMLHGHKAEPLHLCHRLDKETTGVMVLAWDKDMAHQVQELFRTRQVVKKYWAITVHVPMPSAGVVDIPIVEKEAQGQQQHHKMTLSPSYRMDDGKMVKVRRSRNAQVAVTQYQVLSSTLSSALVELQPITGIKHQLRVHLSFGLDCPILGDHKYSDWNRLAPQKLSVGTLKKLGLEQSKARYIPLHLHARQLILPALGSGKEELNLVCKLPRFFVHSLHRLRLEMPNEDQNENNEAKCLGAQ

Target ORF information:

RefSeq Version	NM_032795.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032795.3

ORF Insert Sequence:

ATGGCGGCGC CCAGGTGGAG CGCGTCGGGC CCCTGGATCC GGGGAAACGG CCAAGGTTGC 
GGGAGTCTCT TCACTCTCGT CTCAAAGCCA TTTTGTGCCG CTGCCGCTGC CTCTACGGCC 
ATAAATGCCC AGAGATTAGC GGAGAAGCTC CGAGCCCAGA AACGGGAACA AGACACAAAG 
AAGGAGCCGG TGTCCACAAA CGCTGTTCAG CGGAGAGTGC AAGAAATAGT GCGGTTCACA 
CGGCAGCTGC AGCGAGTCCA CCCCAACGTG CTTGCTAAGG CACTGACCCG AGGAATTCTC 
CACCAGGACA AGAACCTTGT GGTCATCAAT AAGCCCTACG GTCTCCCTGT GCATGGTGGC 
CCTGGGGTCC AGCTCTGCAT CACTGATGTA CTACCTATCC TGGCAAAGAT GCTTCATGGC 
CACAAGGCAG AGCCCTTGCA TCTGTGCCAC CGGCTGGACA AGGAAACCAC AGGTGTAATG 
GTGTTGGCTT GGGACAAGGA CATGGCACAT CAAGTCCAAG AGTTGTTTAG AACCCGTCAG 
GTGGTGAAGA AGTACTGGGC CATCACTGTG CATGTCCCCA TGCCCTCAGC AGGAGTCGTG 
GACATCCCCA TTGTGGAGAA GGAGGCGCAA GGCCAGCAGC AACACCACAA GATGACATTG 
TCCCCGAGCT ACCGCATGGA CGATGGGAAA ATGGTGAAAG TGCGGCGCAG CCGGAATGCG 
CAAGTTGCTG TAACTCAGTA CCAGGTGCTC AGCAGCACTC TCTCCTCCGC CCTCGTGGAG 
CTCCAGCCCA TCACTGGAAT AAAACATCAG CTTCGAGTTC ACTTGTCTTT TGGATTGGAT 
TGTCCAATCC TTGGTGATCA CAAGTACTCA GACTGGAATA GGTTGGCCCC CCAGAAGCTG 
TCTGTGGGCA CCCTGAAGAA GCTGGGGCTA GAACAGTCGA AGGCCCGCTA CATCCCCCTT 
CACCTGCACG CCCGGCAGCT GATCCTGCCT GCCCTGGGGT CCGGGAAGGA GGAACTCAAC 
TTGGTCTGCA AACTTCCTCG CTTCTTTGTG CATTCCCTGC ACCGCCTGCG TTTAGAGATG 
CCAAATGAGG ATCAAAATGA GAACAATGAA GCCAAGTGTC TGGGAGCACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPUSD4 ( XM_011543039.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541341.1 Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu73805
Clone ID Related Accession (Same CDS sequence)	XM_011543039.2
Accession Version	XM_011543039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitochondrial RNA pseudouridine synthase RPUSD4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543039.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCAG CAGGAGTCGT GGACATCCCC ATTGTGGAGA AGGAGGCGCA AGGCCAGCAG 
CAACACCACA AGATGACATT GTCCCCGAGC TACCGCATGG ACGATGGGAA AATGGTGAAA 
GTGCGGCGCA GCCGGAATGC GCAAGTTGCT GTAACTCAGT ACCAGGTGCT CAGCAGCACT 
CTCTCCTCCG CCCTCGTGGA GCTCCAGCCC ATCACTGGAA TAAAACATCA GCTTCGAGTT 
CACTTGTCTT TTGGATTGGA TTGTCCAATC CTTGGTGATC ACAAGTACTC AGACTGGAAT 
AGGTTGGCCC CCCAGAAGCT GTCTGTGGGC ACCCTGAAGA AGCTGGGGCT AGAACAGTCG 
AAGGCCCGCT ACATCCCCCT TCACCTGCAC GCCCGGCAGC TGATCCTGCC TGCCCTGGGG 
TCCGGGAAGG AGGAACTCAA CTTGGTCTGC AAACTTCCTC GCTTCTTTGT GCATTCCCTG 
CACCGCCTGC GTTTAGAGAT GCCAAATGAG GATCAAAATG AGAACAATGA AGCCAAGTGT 
CTGGGAGCAC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541341.1
CDS	631..1185
Translation	MPSAGVVDIPIVEKEAQGQQQHHKMTLSPSYRMDDGKMVKVRRSRNAQVAVTQYQVLSSTLSSALVELQPITGIKHQLRVHLSFGLDCPILGDHKYSDWNRLAPQKLSVGTLKKLGLEQSKARYIPLHLHARQLILPALGSGKEELNLVCKLPRFFVHSLHRLRLEMPNEDQNENNEAKCLGAQ

Target ORF information:

RefSeq Version	XM_011543039.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543039.2

ORF Insert Sequence:

ATGCCCTCAG CAGGAGTCGT GGACATCCCC ATTGTGGAGA AGGAGGCGCA AGGCCAGCAG 
CAACACCACA AGATGACATT GTCCCCGAGC TACCGCATGG ACGATGGGAA AATGGTGAAA 
GTGCGGCGCA GCCGGAATGC GCAAGTTGCT GTAACTCAGT ACCAGGTGCT CAGCAGCACT 
CTCTCCTCCG CCCTCGTGGA GCTCCAGCCC ATCACTGGAA TAAAACATCA GCTTCGAGTT 
CACTTGTCTT TTGGATTGGA TTGTCCAATC CTTGGTGATC ACAAGTACTC AGACTGGAAT 
AGGTTGGCCC CCCAGAAGCT GTCTGTGGGC ACCCTGAAGA AGCTGGGGCT AGAACAGTCG 
AAGGCCCGCT ACATCCCCCT TCACCTGCAC GCCCGGCAGC TGATCCTGCC TGCCCTGGGG 
TCCGGGAAGG AGGAACTCAA CTTGGTCTGC AAACTTCCTC GCTTCTTTGT GCATTCCCTG 
CACCGCCTGC GTTTAGAGAT GCCAAATGAG GATCAAAATG AGAACAATGA AGCCAAGTGT 
CTGGGAGCAC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPUSD4 ( NM_001144827.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138299.1 Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02254
Clone ID Related Accession (Same CDS sequence)	NM_001144827.1
Accession Version	NM_001144827.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-13
Organism	Homo sapiens(human)
Product	mitochondrial RNA pseudouridine synthase RPUSD4 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB051034.1, AK308059.1, BE260782.1 and BU679173.1. Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the coding region, compared to variant 1. This results in a shorter protein (isoform b) compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AK308059.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2162946 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGC CCAGGTGGAG CGCGTCGGGC CCCTGGATCC GGGGAAACGG CCAAGGTTGC 
GGGAGTCTCT TCACTCTCGT CTCAAAGCCA TTTTGTGCCG CTGCCGCTGC CTCTACGGCC 
ATAAATGCCC AGAGATTAGC GGAGAAGCTC CGAGCCCAGA AACGGGAACA AGACACAAAG 
AAGGAGCCGG TGTCCACAAA CGCTGTTCAG CGGAGAGTGC AAGAAATAGT GCGGTTCACA 
CGGCAGCTGC AGCGAGTCCA CCCCAACGTG CTTGCTAAGG CACTGACCCG AGGAATTCTC 
CACCAGGACA AGAACCTTGT GGTCATCAAT AAGCCCTACG GTCTCCCTGT GCATGGTGGC 
CCTGGGGTCC AGCTCTGCAT CACTGATGTA CTACCTATCC TGGCAAAGAT GCTTCATGGC 
CACAAGGCAG AGCCCTTGCA TCTGTGCCAC CGGCTGGACA AGGAAACCAC AGGTGTAATG 
GTGTTGGCTT GGGACAAGGA CATGGCACAT CAAGTCCAAG AGTTGTTTAG AACCCGTCAG 
GTGGTGAAGA AGTACTGGGC CATCACTGTG CATGTCCCCA TGCCCTCAGC AGGAGTCGTG 
GACATCCCCA TTGTGGAGAA GGAGGCGCAA GTTGCTGTAA CTCAGTACCA GGTGCTCAGC 
AGCACTCTCT CCTCCGCCCT CGTGGAGCTC CAGCCCATCA CTGGAATAAA ACATCAGCTT 
CGAGTTCACT TGTCTTTTGG ATTGGATTGT CCAATCCTTG GTGATCACAA GTACTCAGAC 
TGGAATAGGT TGGCCCCCCA GAAGCTGTCT GTGGGCACCC TGAAGAAGCT GGGGCTAGAA 
CAGTCGAAGG CCCGCTACAT CCCCCTTCAC CTGCACGCCC GGCAGCTGAT CCTGCCTGCC 
CTGGGGTCCG GGAAGGAGGA ACTCAACTTG GTCTGCAAAC TTCCTCGCTT CTTTGTGCAT 
TCCCTGCACC GCCTGCGTTT AGAGATGCCA AATGAGGATC AAAATGAGAA CAATGAAGCC 
AAGTGTCTGG GAGCACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138299.1
CDS	55..1095
Translation	MAAPRWSASGPWIRGNGQGCGSLFTLVSKPFCAAAAASTAINAQRLAEKLRAQKREQDTKKEPVSTNAVQRRVQEIVRFTRQLQRVHPNVLAKALTRGILHQDKNLVVINKPYGLPVHGGPGVQLCITDVLPILAKMLHGHKAEPLHLCHRLDKETTGVMVLAWDKDMAHQVQELFRTRQVVKKYWAITVHVPMPSAGVVDIPIVEKEAQVAVTQYQVLSSTLSSALVELQPITGIKHQLRVHLSFGLDCPILGDHKYSDWNRLAPQKLSVGTLKKLGLEQSKARYIPLHLHARQLILPALGSGKEELNLVCKLPRFFVHSLHRLRLEMPNEDQNENNEAKCLGAQ

Target ORF information:

RefSeq Version	NM_001144827.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144827.1

ORF Insert Sequence:

ATGGCGGCGC CCAGGTGGAG CGCGTCGGGC CCCTGGATCC GGGGAAACGG CCAAGGTTGC 
GGGAGTCTCT TCACTCTCGT CTCAAAGCCA TTTTGTGCCG CTGCCGCTGC CTCTACGGCC 
ATAAATGCCC AGAGATTAGC GGAGAAGCTC CGAGCCCAGA AACGGGAACA AGACACAAAG 
AAGGAGCCGG TGTCCACAAA CGCTGTTCAG CGGAGAGTGC AAGAAATAGT GCGGTTCACA 
CGGCAGCTGC AGCGAGTCCA CCCCAACGTG CTTGCTAAGG CACTGACCCG AGGAATTCTC 
CACCAGGACA AGAACCTTGT GGTCATCAAT AAGCCCTACG GTCTCCCTGT GCATGGTGGC 
CCTGGGGTCC AGCTCTGCAT CACTGATGTA CTACCTATCC TGGCAAAGAT GCTTCATGGC 
CACAAGGCAG AGCCCTTGCA TCTGTGCCAC CGGCTGGACA AGGAAACCAC AGGTGTAATG 
GTGTTGGCTT GGGACAAGGA CATGGCACAT CAAGTCCAAG AGTTGTTTAG AACCCGTCAG 
GTGGTGAAGA AGTACTGGGC CATCACTGTG CATGTCCCCA TGCCCTCAGC AGGAGTCGTG 
GACATCCCCA TTGTGGAGAA GGAGGCGCAA GTTGCTGTAA CTCAGTACCA GGTGCTCAGC 
AGCACTCTCT CCTCCGCCCT CGTGGAGCTC CAGCCCATCA CTGGAATAAA ACATCAGCTT 
CGAGTTCACT TGTCTTTTGG ATTGGATTGT CCAATCCTTG GTGATCACAA GTACTCAGAC 
TGGAATAGGT TGGCCCCCCA GAAGCTGTCT GTGGGCACCC TGAAGAAGCT GGGGCTAGAA 
CAGTCGAAGG CCCGCTACAT CCCCCTTCAC CTGCACGCCC GGCAGCTGAT CCTGCCTGCC 
CTGGGGTCCG GGAAGGAGGA ACTCAACTTG GTCTGCAAAC TTCCTCGCTT CTTTGTGCAT 
TCCCTGCACC GCCTGCGTTT AGAGATGCCA AATGAGGATC AAAATGAGAA CAATGAAGCC 
AAGTGTCTGG GAGCACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPUSD4 ( NM_001363516.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350445.1 Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant 3, mRNA.

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CloneID	OHu106868
Clone ID Related Accession (Same CDS sequence)	NM_001363516.1 , NM_001363516.2
Accession Version	NM_001363516.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-13
Organism	Homo sapiens(human)
Product	mitochondrial RNA pseudouridine synthase RPUSD4 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP001893.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.67213.1, SRR3476690.183090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGC CCAGGTGGAG CGCGTCGGGC CCCTGGATCC GGGGAAACGG CCAAGGTTGC 
GGGAGTCTCT TCACTCTCGT CTCAAAGCCA TTTTGTGCCG CTGCCGCTGC CTCTACGGCC 
ATAAATGCCC AGAGATTAGC GGAGAAGCTC CGAGCCCAGA AACGGGAACA AGACACAAAG 
AAGGAGCCGG TGTCCACAAA CGCTGTTCAG CGGAGAGTGC AAGAAATAGT GCGGTTCACA 
CGGCAGCTGC AGCGAGTCCA CCCCAACGTG CTTGCTAAGG CACTGACCCG AGGAATTCTC 
CACCAGGACA AGAACCTTGT GGTCATCAAT AAGCCCTACG GTCTCCCTGT GCATGGTGGC 
CCTGGGGTCC AGCTCTGCAT CACTGATGTA CTACCTATCC TGGCAAAGAT GCTTCATGGC 
CACAAGGCAG AGCCCTTGCA TCTGTGCCAC CGGCTGGACA AGGAAACCAC AGGTGTAATG 
GTGTTGGCTT GGGACAAGGA CATGGCACAT CAAGTCCAAG AGTTGTTTAG AACCCGTCAG 
GTGGTGAAGA AGTACTGGGC CATCACTGTG CATGTCCCCA TGCCCTCAGC AGGAGTCGTG 
GACATCCCCA TTGTGGAGAA GGAGGCGCAA GGCCAGCAGC AACACCACAA GATGACATTG 
TCCCCGAGCT ACCGCATGGA CGATGGGAAA ATGGTGAAAG TGCGGCGCAG CCGGAATGCG 
CAAGTTGCTG TAACTCAGTA CCAGGTGCTC AGCAGCACTC TCTCCTCCGC CCTCGTGGAG 
CTCCAGCCCA TCACTGGAAT AAAACATCAG CTTCGAGTTC ACTTGTCTTT TGGATTGGAT 
TGTCCAATCC TTGGTGATCA CAAGTACTCA GACTGGAATA GGTTGGCCCC CCAGCTGTCT 
GTGGGCACCC TGAAGAAGCT GGGGCTAGAA CAGTCGAAGG CCCGCTACAT CCCCCTTCAC 
CTGCACGCCC GGCAGCTGAT CCTGCCTGCC CTGGGGTCCG GGAAGGAGGA ACTCAACTTG 
GTCTGCAAAC TTCCTCGCTT CTTTGTGCAT TCCCTGCACC GCCTGCGTTT AGAGATGCCA 
AATGAGGATC AAAATGAGAA CAATGAAGCC AAGTGTCTGG GAGCACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350445.1
CDS	55..1185
Translation	MAAPRWSASGPWIRGNGQGCGSLFTLVSKPFCAAAAASTAINAQRLAEKLRAQKREQDTKKEPVSTNAVQRRVQEIVRFTRQLQRVHPNVLAKALTRGILHQDKNLVVINKPYGLPVHGGPGVQLCITDVLPILAKMLHGHKAEPLHLCHRLDKETTGVMVLAWDKDMAHQVQELFRTRQVVKKYWAITVHVPMPSAGVVDIPIVEKEAQGQQQHHKMTLSPSYRMDDGKMVKVRRSRNAQVAVTQYQVLSSTLSSALVELQPITGIKHQLRVHLSFGLDCPILGDHKYSDWNRLAPQLSVGTLKKLGLEQSKARYIPLHLHARQLILPALGSGKEELNLVCKLPRFFVHSLHRLRLEMPNEDQNENNEAKCLGAQ

Target ORF information:

RefSeq Version	NM_001363516.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363516.1

ORF Insert Sequence:

ATGGCGGCGC CCAGGTGGAG CGCGTCGGGC CCCTGGATCC GGGGAAACGG CCAAGGTTGC 
GGGAGTCTCT TCACTCTCGT CTCAAAGCCA TTTTGTGCCG CTGCCGCTGC CTCTACGGCC 
ATAAATGCCC AGAGATTAGC GGAGAAGCTC CGAGCCCAGA AACGGGAACA AGACACAAAG 
AAGGAGCCGG TGTCCACAAA CGCTGTTCAG CGGAGAGTGC AAGAAATAGT GCGGTTCACA 
CGGCAGCTGC AGCGAGTCCA CCCCAACGTG CTTGCTAAGG CACTGACCCG AGGAATTCTC 
CACCAGGACA AGAACCTTGT GGTCATCAAT AAGCCCTACG GTCTCCCTGT GCATGGTGGC 
CCTGGGGTCC AGCTCTGCAT CACTGATGTA CTACCTATCC TGGCAAAGAT GCTTCATGGC 
CACAAGGCAG AGCCCTTGCA TCTGTGCCAC CGGCTGGACA AGGAAACCAC AGGTGTAATG 
GTGTTGGCTT GGGACAAGGA CATGGCACAT CAAGTCCAAG AGTTGTTTAG AACCCGTCAG 
GTGGTGAAGA AGTACTGGGC CATCACTGTG CATGTCCCCA TGCCCTCAGC AGGAGTCGTG 
GACATCCCCA TTGTGGAGAA GGAGGCGCAA GGCCAGCAGC AACACCACAA GATGACATTG 
TCCCCGAGCT ACCGCATGGA CGATGGGAAA ATGGTGAAAG TGCGGCGCAG CCGGAATGCG 
CAAGTTGCTG TAACTCAGTA CCAGGTGCTC AGCAGCACTC TCTCCTCCGC CCTCGTGGAG 
CTCCAGCCCA TCACTGGAAT AAAACATCAG CTTCGAGTTC ACTTGTCTTT TGGATTGGAT 
TGTCCAATCC TTGGTGATCA CAAGTACTCA GACTGGAATA GGTTGGCCCC CCAGCTGTCT 
GTGGGCACCC TGAAGAAGCT GGGGCTAGAA CAGTCGAAGG CCCGCTACAT CCCCCTTCAC 
CTGCACGCCC GGCAGCTGAT CCTGCCTGCC CTGGGGTCCG GGAAGGAGGA ACTCAACTTG 
GTCTGCAAAC TTCCTCGCTT CTTTGTGCAT TCCCTGCACC GCCTGCGTTT AGAGATGCCA 
AATGAGGATC AAAATGAGAA CAATGAAGCC AAGTGTCTGG GAGCACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPUSD4 ( NM_001363516.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350445.1 Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu106868
Clone ID Related Accession (Same CDS sequence)	NM_001363516.1 , NM_001363516.2
Accession Version	NM_001363516.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	mitochondrial RNA pseudouridine synthase RPUSD4 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP001893.5. On May 31, 2019 this sequence version replaced NM_001363516.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.183090.1, SRR1660803.67213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGC CCAGGTGGAG CGCGTCGGGC CCCTGGATCC GGGGAAACGG CCAAGGTTGC 
GGGAGTCTCT TCACTCTCGT CTCAAAGCCA TTTTGTGCCG CTGCCGCTGC CTCTACGGCC 
ATAAATGCCC AGAGATTAGC GGAGAAGCTC CGAGCCCAGA AACGGGAACA AGACACAAAG 
AAGGAGCCGG TGTCCACAAA CGCTGTTCAG CGGAGAGTGC AAGAAATAGT GCGGTTCACA 
CGGCAGCTGC AGCGAGTCCA CCCCAACGTG CTTGCTAAGG CACTGACCCG AGGAATTCTC 
CACCAGGACA AGAACCTTGT GGTCATCAAT AAGCCCTACG GTCTCCCTGT GCATGGTGGC 
CCTGGGGTCC AGCTCTGCAT CACTGATGTA CTACCTATCC TGGCAAAGAT GCTTCATGGC 
CACAAGGCAG AGCCCTTGCA TCTGTGCCAC CGGCTGGACA AGGAAACCAC AGGTGTAATG 
GTGTTGGCTT GGGACAAGGA CATGGCACAT CAAGTCCAAG AGTTGTTTAG AACCCGTCAG 
GTGGTGAAGA AGTACTGGGC CATCACTGTG CATGTCCCCA TGCCCTCAGC AGGAGTCGTG 
GACATCCCCA TTGTGGAGAA GGAGGCGCAA GGCCAGCAGC AACACCACAA GATGACATTG 
TCCCCGAGCT ACCGCATGGA CGATGGGAAA ATGGTGAAAG TGCGGCGCAG CCGGAATGCG 
CAAGTTGCTG TAACTCAGTA CCAGGTGCTC AGCAGCACTC TCTCCTCCGC CCTCGTGGAG 
CTCCAGCCCA TCACTGGAAT AAAACATCAG CTTCGAGTTC ACTTGTCTTT TGGATTGGAT 
TGTCCAATCC TTGGTGATCA CAAGTACTCA GACTGGAATA GGTTGGCCCC CCAGCTGTCT 
GTGGGCACCC TGAAGAAGCT GGGGCTAGAA CAGTCGAAGG CCCGCTACAT CCCCCTTCAC 
CTGCACGCCC GGCAGCTGAT CCTGCCTGCC CTGGGGTCCG GGAAGGAGGA ACTCAACTTG 
GTCTGCAAAC TTCCTCGCTT CTTTGTGCAT TCCCTGCACC GCCTGCGTTT AGAGATGCCA 
AATGAGGATC AAAATGAGAA CAATGAAGCC AAGTGTCTGG GAGCACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350445.1
CDS	13..1143
Translation	MAAPRWSASGPWIRGNGQGCGSLFTLVSKPFCAAAAASTAINAQRLAEKLRAQKREQDTKKEPVSTNAVQRRVQEIVRFTRQLQRVHPNVLAKALTRGILHQDKNLVVINKPYGLPVHGGPGVQLCITDVLPILAKMLHGHKAEPLHLCHRLDKETTGVMVLAWDKDMAHQVQELFRTRQVVKKYWAITVHVPMPSAGVVDIPIVEKEAQGQQQHHKMTLSPSYRMDDGKMVKVRRSRNAQVAVTQYQVLSSTLSSALVELQPITGIKHQLRVHLSFGLDCPILGDHKYSDWNRLAPQLSVGTLKKLGLEQSKARYIPLHLHARQLILPALGSGKEELNLVCKLPRFFVHSLHRLRLEMPNEDQNENNEAKCLGAQ

Target ORF information:

RefSeq Version	NM_001363516.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA pseudouridine synthase D4 (RPUSD4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363516.2

ORF Insert Sequence:

ATGGCGGCGC CCAGGTGGAG CGCGTCGGGC CCCTGGATCC GGGGAAACGG CCAAGGTTGC 
GGGAGTCTCT TCACTCTCGT CTCAAAGCCA TTTTGTGCCG CTGCCGCTGC CTCTACGGCC 
ATAAATGCCC AGAGATTAGC GGAGAAGCTC CGAGCCCAGA AACGGGAACA AGACACAAAG 
AAGGAGCCGG TGTCCACAAA CGCTGTTCAG CGGAGAGTGC AAGAAATAGT GCGGTTCACA 
CGGCAGCTGC AGCGAGTCCA CCCCAACGTG CTTGCTAAGG CACTGACCCG AGGAATTCTC 
CACCAGGACA AGAACCTTGT GGTCATCAAT AAGCCCTACG GTCTCCCTGT GCATGGTGGC 
CCTGGGGTCC AGCTCTGCAT CACTGATGTA CTACCTATCC TGGCAAAGAT GCTTCATGGC 
CACAAGGCAG AGCCCTTGCA TCTGTGCCAC CGGCTGGACA AGGAAACCAC AGGTGTAATG 
GTGTTGGCTT GGGACAAGGA CATGGCACAT CAAGTCCAAG AGTTGTTTAG AACCCGTCAG 
GTGGTGAAGA AGTACTGGGC CATCACTGTG CATGTCCCCA TGCCCTCAGC AGGAGTCGTG 
GACATCCCCA TTGTGGAGAA GGAGGCGCAA GGCCAGCAGC AACACCACAA GATGACATTG 
TCCCCGAGCT ACCGCATGGA CGATGGGAAA ATGGTGAAAG TGCGGCGCAG CCGGAATGCG 
CAAGTTGCTG TAACTCAGTA CCAGGTGCTC AGCAGCACTC TCTCCTCCGC CCTCGTGGAG 
CTCCAGCCCA TCACTGGAAT AAAACATCAG CTTCGAGTTC ACTTGTCTTT TGGATTGGAT 
TGTCCAATCC TTGGTGATCA CAAGTACTCA GACTGGAATA GGTTGGCCCC CCAGCTGTCT 
GTGGGCACCC TGAAGAAGCT GGGGCTAGAA CAGTCGAAGG CCCGCTACAT CCCCCTTCAC 
CTGCACGCCC GGCAGCTGAT CCTGCCTGCC CTGGGGTCCG GGAAGGAGGA ACTCAACTTG 
GTCTGCAAAC TTCCTCGCTT CTTTGTGCAT TCCCTGCACC GCCTGCGTTT AGAGATGCCA 
AATGAGGATC AAAATGAGAA CAATGAAGCC AAGTGTCTGG GAGCACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules.
The Journal of biological chemistry292(11)4519-4532(2017 03)
Zaganelli S,Rebelo-Guiomar P,Maundrell K,Rozanska A,Pierredon S,Powell CA,Jourdain AA,Hulo N,Lightowlers RN,Chrzanowska-Lightowlers ZM,Minczuk M,Martinou JC

A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.
Cell metabolism24(6)875-885(2016 Dec)
Arroyo JD,Jourdain AA,Calvo SE,Ballarano CA,Doench JG,Root DE,Mootha VK

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

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RPUSD4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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