ITCH gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011529078.1	XP_011527380.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
NM_001257137.1	NP_001244066.1	E3 ubiquitin-protein ligase Itchy homolog isoform 1
NM_001257138.2	NP_001244067.1	E3 ubiquitin-protein ligase Itchy homolog isoform 3
NM_001324197.1	NP_001311126.1	E3 ubiquitin-protein ligase Itchy homolog isoform 1
XM_024452005.1	XP_024307773.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
XM_005260570.1	XP_005260627.1	E3 ubiquitin-protein ligase Itchy homolog isoform X4
XM_017028092.2	XP_016883581.1	E3 ubiquitin-protein ligase Itchy homolog isoform X6
NM_001257137.3	NP_001244066.1	E3 ubiquitin-protein ligase Itchy homolog isoform 1
NM_001324198.2	NP_001311127.1	E3 ubiquitin-protein ligase Itchy homolog isoform 2
NM_001257138.1	NP_001244067.1	E3 ubiquitin-protein ligase Itchy homolog isoform 3
XM_011529077.1	XP_011527379.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
NM_031483.6	NP_113671.3	E3 ubiquitin-protein ligase Itchy homolog isoform 2
NM_001324198.1	NP_001311127.1	E3 ubiquitin-protein ligase Itchy homolog isoform 2
XM_017028092.1	XP_016883581.1	E3 ubiquitin-protein ligase Itchy homolog isoform X6
NM_001324197.2	NP_001311126.1	E3 ubiquitin-protein ligase Itchy homolog isoform 1
XM_017028090.1	XP_016883579.1	E3 ubiquitin-protein ligase Itchy homolog isoform X2
XM_011529079.1	XP_011527381.1	E3 ubiquitin-protein ligase Itchy homolog isoform X5
NM_001257137.2	NP_001244066.1	E3 ubiquitin-protein ligase Itchy homolog isoform 1
XM_017028089.1	XP_016883578.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
XM_017028091.1	XP_016883580.1	E3 ubiquitin-protein ligase Itchy homolog isoform X3
NM_031483.5	NP_113671.3	E3 ubiquitin-protein ligase Itchy homolog isoform 2
NM_001257138.3	NP_001244067.1	E3 ubiquitin-protein ligase Itchy homolog isoform 3
NM_031483.7	NP_113671.3	E3 ubiquitin-protein ligase Itchy homolog isoform 2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22317	NM_001324198.2 Latest version!	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$434.50-$608.30 $869.00
OHu22317	NM_031483.7 Latest version!	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$434.50-$608.30 $869.00
OHu22837	NM_001257137.3 Latest version!	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu22837	NM_001324197.2 Latest version!	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu23120	NM_001257138.3 Latest version!	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu22837	XM_017028089.1 Latest version!	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu22837	XM_011529077.1 Latest version!	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu22837	XM_011529078.1 Latest version!	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu22837	XM_024452005.1 Latest version!	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu23120	XM_017028091.1 Latest version!	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu93131	XM_017028092.2 Latest version!	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu53122	XM_005260570.1 Latest version!	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu73156	XM_011529079.1 Latest version!	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu93130	XM_017028090.1 Latest version!	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu22837	NM_001257137.1	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu23120	NM_001257138.1	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu22317	NM_001324198.1	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$434.50-$608.30 $869.00
OHu93131	XM_017028092.1	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu22837	NM_001324197.1	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu22837	NM_001257137.2	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu22317	NM_031483.5	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$434.50-$608.30 $869.00
OHu23120	NM_001257138.2	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu22317	NM_031483.6	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$434.50-$608.30 $869.00
Hide Old Accession Versions >>

ITCH ( NM_001324198.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311127.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu22317
Clone ID Related Accession (Same CDS sequence)	NM_001324198.2 , NM_031483.6 , NM_001324198.1 , NM_031483.5 , NM_031483.7
Accession Version	NM_001324198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL109923.29 and BG928040.1. On Jun 2, 2019 this sequence version replaced NM_001324198.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.17759.1, SRR1660807.164506.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCTCAGAATG ATGATGGCTC CAGATCCAAG GATGAAACAA GAGTGAGCAC AAATGGATCA 
GATGACCCTG AAGATGCAGG AGCTGGTGAA AATAGGAGAG TCAGTGGGAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGACCTCCAA GACCTTCACG ACCACCACCA 
CCCACCCCAC GTAGACCAGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGGTCTAGTA CAGGCTCTCT GCCGCCGACA AATACAAATA CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTAACTC AAGCTCCCTT GCCACCTGGT TGGGAGCAGA GAGTGGACCA GCACGGGCGA 
GTTTACTATG TAGATCATGT TGAGAAAAGA ACAACATGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCCGTCC GGAACTATGA ACAATGGCAG 
CTACAGCGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT CATTTATGGG 
AATCAAGATT TATTTGCTAC ATCACAAAGT AAAGAATTTG ATCCTCTTGG TCCATTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGCAGAGTAT ATTTCGTCAA CCACAACACA 
CGAATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGTTGGG AAATGAGATT CACAGTGGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACTACCA CCTATATAGA TCCCCGCACA GGAAAATCTG CCCTAGACAA TGGACCTCAG 
ATAGCCTATG TTCGGGACTT CAAAGCAAAG GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAACAGA TAATGAGCTT CAGTCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAAGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGATTTA TTGCCATGGC TCTGTTCCAT GGGAAATTCA TAGACACGGG TTTTTCTTTA 
CCATTCTATA AGCGTATCTT GAACAAACCA GTTGGACTCA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAGGAAAACA ATATTGAGGA ATGTGATTTG 
GAAATGTACT TCTCCGTTGA CAAAGAAATT CTAGGTGAAA TTAAGAGTCA TGATCTGAAA 
CCTAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATACAT CAGAATGGTA 
GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA GAACAGACAC AAGCTTTCTT TGAAGGCTTT 
AATGAAATTC TTCCCCAGCA ATATTTGCAA TACTTTGATG CAAAGGAATT AGAGGTCCTT 
TTATGTGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TATGCAAGGA CCAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTAGGAGGA 
TTTGCTGATC TCATGGGGAG CAATGGACCA CAGAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGC TACCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCATACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTG TTTGCCATAG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311127.1
CDS	197..2785
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001324198.2
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324198.2

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCTCAGAATG ATGATGGCTC CAGATCCAAG GATGAAACAA GAGTGAGCAC AAATGGATCA 
GATGACCCTG AAGATGCAGG AGCTGGTGAA AATAGGAGAG TCAGTGGGAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGACCTCCAA GACCTTCACG ACCACCACCA 
CCCACCCCAC GTAGACCAGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGGTCTAGTA CAGGCTCTCT GCCGCCGACA AATACAAATA CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTAACTC AAGCTCCCTT GCCACCTGGT TGGGAGCAGA GAGTGGACCA GCACGGGCGA 
GTTTACTATG TAGATCATGT TGAGAAAAGA ACAACATGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCCGTCC GGAACTATGA ACAATGGCAG 
CTACAGCGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT CATTTATGGG 
AATCAAGATT TATTTGCTAC ATCACAAAGT AAAGAATTTG ATCCTCTTGG TCCATTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGCAGAGTAT ATTTCGTCAA CCACAACACA 
CGAATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGTTGGG AAATGAGATT CACAGTGGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACTACCA CCTATATAGA TCCCCGCACA GGAAAATCTG CCCTAGACAA TGGACCTCAG 
ATAGCCTATG TTCGGGACTT CAAAGCAAAG GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAACAGA TAATGAGCTT CAGTCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAAGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGATTTA TTGCCATGGC TCTGTTCCAT GGGAAATTCA TAGACACGGG TTTTTCTTTA 
CCATTCTATA AGCGTATCTT GAACAAACCA GTTGGACTCA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAGGAAAACA ATATTGAGGA ATGTGATTTG 
GAAATGTACT TCTCCGTTGA CAAAGAAATT CTAGGTGAAA TTAAGAGTCA TGATCTGAAA 
CCTAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATACAT CAGAATGGTA 
GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA GAACAGACAC AAGCTTTCTT TGAAGGCTTT 
AATGAAATTC TTCCCCAGCA ATATTTGCAA TACTTTGATG CAAAGGAATT AGAGGTCCTT 
TTATGTGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TATGCAAGGA CCAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTAGGAGGA 
TTTGCTGATC TCATGGGGAG CAATGGACCA CAGAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGC TACCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCATACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTG TTTGCCATAG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( NM_031483.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_113671.3 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22317
Clone ID Related Accession (Same CDS sequence)	NM_001324198.2 , NM_031483.6 , NM_001324198.1 , NM_031483.5 , NM_031483.7
Accession Version	NM_031483.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL705688.1, AK315212.1, AL109923.29, AA730741.1 and BG928040.1. On Apr 28, 2016 this sequence version replaced NM_031483.5. Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Both variants 2 and 5 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.130987.1, SRR1660809.192411.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCTCAGAATG ATGATGGCTC CAGATCCAAG GATGAAACAA GAGTGAGCAC AAATGGATCA 
GATGACCCTG AAGATGCAGG AGCTGGTGAA AATAGGAGAG TCAGTGGGAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGACCTCCAA GACCTTCACG ACCACCACCA 
CCCACCCCAC GTAGACCAGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGGTCTAGTA CAGGCTCTCT GCCGCCGACA AATACAAATA CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTAACTC AAGCTCCCTT GCCACCTGGT TGGGAGCAGA GAGTGGACCA GCACGGGCGA 
GTTTACTATG TAGATCATGT TGAGAAAAGA ACAACATGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCCGTCC GGAACTATGA ACAATGGCAG 
CTACAGCGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT CATTTATGGG 
AATCAAGATT TATTTGCTAC ATCACAAAGT AAAGAATTTG ATCCTCTTGG TCCATTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGCAGAGTAT ATTTCGTCAA CCACAACACA 
CGAATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGTTGGG AAATGAGATT CACAGTGGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACTACCA CCTATATAGA TCCCCGCACA GGAAAATCTG CCCTAGACAA TGGACCTCAG 
ATAGCCTATG TTCGGGACTT CAAAGCAAAG GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAACAGA TAATGAGCTT CAGTCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAAGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGATTTA TTGCCATGGC TCTGTTCCAT GGGAAATTCA TAGACACGGG TTTTTCTTTA 
CCATTCTATA AGCGTATCTT GAACAAACCA GTTGGACTCA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAGGAAAACA ATATTGAGGA ATGTGATTTG 
GAAATGTACT TCTCCGTTGA CAAAGAAATT CTAGGTGAAA TTAAGAGTCA TGATCTGAAA 
CCTAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATACAT CAGAATGGTA 
GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA GAACAGACAC AAGCTTTCTT TGAAGGCTTT 
AATGAAATTC TTCCCCAGCA ATATTTGCAA TACTTTGATG CAAAGGAATT AGAGGTCCTT 
TTATGTGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TATGCAAGGA CCAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTAGGAGGA 
TTTGCTGATC TCATGGGGAG CAATGGACCA CAGAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGC TACCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCATACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTG TTTGCCATAG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113671.3
CDS	214..2802
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_031483.6
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031483.6

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCTCAGAATG ATGATGGCTC CAGATCCAAG GATGAAACAA GAGTGAGCAC AAATGGATCA 
GATGACCCTG AAGATGCAGG AGCTGGTGAA AATAGGAGAG TCAGTGGGAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGACCTCCAA GACCTTCACG ACCACCACCA 
CCCACCCCAC GTAGACCAGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGGTCTAGTA CAGGCTCTCT GCCGCCGACA AATACAAATA CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTAACTC AAGCTCCCTT GCCACCTGGT TGGGAGCAGA GAGTGGACCA GCACGGGCGA 
GTTTACTATG TAGATCATGT TGAGAAAAGA ACAACATGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCCGTCC GGAACTATGA ACAATGGCAG 
CTACAGCGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT CATTTATGGG 
AATCAAGATT TATTTGCTAC ATCACAAAGT AAAGAATTTG ATCCTCTTGG TCCATTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGCAGAGTAT ATTTCGTCAA CCACAACACA 
CGAATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGTTGGG AAATGAGATT CACAGTGGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACTACCA CCTATATAGA TCCCCGCACA GGAAAATCTG CCCTAGACAA TGGACCTCAG 
ATAGCCTATG TTCGGGACTT CAAAGCAAAG GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAACAGA TAATGAGCTT CAGTCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAAGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGATTTA TTGCCATGGC TCTGTTCCAT GGGAAATTCA TAGACACGGG TTTTTCTTTA 
CCATTCTATA AGCGTATCTT GAACAAACCA GTTGGACTCA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAGGAAAACA ATATTGAGGA ATGTGATTTG 
GAAATGTACT TCTCCGTTGA CAAAGAAATT CTAGGTGAAA TTAAGAGTCA TGATCTGAAA 
CCTAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATACAT CAGAATGGTA 
GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA GAACAGACAC AAGCTTTCTT TGAAGGCTTT 
AATGAAATTC TTCCCCAGCA ATATTTGCAA TACTTTGATG CAAAGGAATT AGAGGTCCTT 
TTATGTGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TATGCAAGGA CCAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTAGGAGGA 
TTTGCTGATC TCATGGGGAG CAATGGACCA CAGAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGC TACCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCATACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTG TTTGCCATAG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( NM_001324198.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311127.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 5, mRNA.

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CloneID	OHu22317
Clone ID Related Accession (Same CDS sequence)	NM_001324198.2 , NM_031483.6 , NM_001324198.1 , NM_031483.5 , NM_031483.7
Accession Version	NM_001324198.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL705688.1, AL356299.16, AL109923.29 and BG928040.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.164506.1, SRR1803614.17759.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCTCAGAATG ATGATGGCTC CAGATCCAAG GATGAAACAA GAGTGAGCAC AAATGGATCA 
GATGACCCTG AAGATGCAGG AGCTGGTGAA AATAGGAGAG TCAGTGGGAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGACCTCCAA GACCTTCACG ACCACCACCA 
CCCACCCCAC GTAGACCAGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGGTCTAGTA CAGGCTCTCT GCCGCCGACA AATACAAATA CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTAACTC AAGCTCCCTT GCCACCTGGT TGGGAGCAGA GAGTGGACCA GCACGGGCGA 
GTTTACTATG TAGATCATGT TGAGAAAAGA ACAACATGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCCGTCC GGAACTATGA ACAATGGCAG 
CTACAGCGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT CATTTATGGG 
AATCAAGATT TATTTGCTAC ATCACAAAGT AAAGAATTTG ATCCTCTTGG TCCATTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGCAGAGTAT ATTTCGTCAA CCACAACACA 
CGAATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGTTGGG AAATGAGATT CACAGTGGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACTACCA CCTATATAGA TCCCCGCACA GGAAAATCTG CCCTAGACAA TGGACCTCAG 
ATAGCCTATG TTCGGGACTT CAAAGCAAAG GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAACAGA TAATGAGCTT CAGTCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAAGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGATTTA TTGCCATGGC TCTGTTCCAT GGGAAATTCA TAGACACGGG TTTTTCTTTA 
CCATTCTATA AGCGTATCTT GAACAAACCA GTTGGACTCA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAGGAAAACA ATATTGAGGA ATGTGATTTG 
GAAATGTACT TCTCCGTTGA CAAAGAAATT CTAGGTGAAA TTAAGAGTCA TGATCTGAAA 
CCTAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATACAT CAGAATGGTA 
GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA GAACAGACAC AAGCTTTCTT TGAAGGCTTT 
AATGAAATTC TTCCCCAGCA ATATTTGCAA TACTTTGATG CAAAGGAATT AGAGGTCCTT 
TTATGTGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TATGCAAGGA CCAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTAGGAGGA 
TTTGCTGATC TCATGGGGAG CAATGGACCA CAGAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGC TACCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCATACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTG TTTGCCATAG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311127.1
CDS	235..2823
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001324198.1
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324198.1

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCTCAGAATG ATGATGGCTC CAGATCCAAG GATGAAACAA GAGTGAGCAC AAATGGATCA 
GATGACCCTG AAGATGCAGG AGCTGGTGAA AATAGGAGAG TCAGTGGGAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGACCTCCAA GACCTTCACG ACCACCACCA 
CCCACCCCAC GTAGACCAGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGGTCTAGTA CAGGCTCTCT GCCGCCGACA AATACAAATA CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTAACTC AAGCTCCCTT GCCACCTGGT TGGGAGCAGA GAGTGGACCA GCACGGGCGA 
GTTTACTATG TAGATCATGT TGAGAAAAGA ACAACATGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCCGTCC GGAACTATGA ACAATGGCAG 
CTACAGCGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT CATTTATGGG 
AATCAAGATT TATTTGCTAC ATCACAAAGT AAAGAATTTG ATCCTCTTGG TCCATTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGCAGAGTAT ATTTCGTCAA CCACAACACA 
CGAATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGTTGGG AAATGAGATT CACAGTGGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACTACCA CCTATATAGA TCCCCGCACA GGAAAATCTG CCCTAGACAA TGGACCTCAG 
ATAGCCTATG TTCGGGACTT CAAAGCAAAG GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAACAGA TAATGAGCTT CAGTCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAAGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGATTTA TTGCCATGGC TCTGTTCCAT GGGAAATTCA TAGACACGGG TTTTTCTTTA 
CCATTCTATA AGCGTATCTT GAACAAACCA GTTGGACTCA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAGGAAAACA ATATTGAGGA ATGTGATTTG 
GAAATGTACT TCTCCGTTGA CAAAGAAATT CTAGGTGAAA TTAAGAGTCA TGATCTGAAA 
CCTAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATACAT CAGAATGGTA 
GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA GAACAGACAC AAGCTTTCTT TGAAGGCTTT 
AATGAAATTC TTCCCCAGCA ATATTTGCAA TACTTTGATG CAAAGGAATT AGAGGTCCTT 
TTATGTGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TATGCAAGGA CCAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTAGGAGGA 
TTTGCTGATC TCATGGGGAG CAATGGACCA CAGAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGC TACCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCATACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTG TTTGCCATAG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( NM_031483.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_113671.3 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22317
Clone ID Related Accession (Same CDS sequence)	NM_001324198.2 , NM_031483.6 , NM_001324198.1 , NM_031483.5 , NM_031483.7
Accession Version	NM_031483.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-02
Organism	Homo sapiens(Human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform 2
Comment

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCTCAGAATG ATGATGGCTC CAGATCCAAG GATGAAACAA GAGTGAGCAC AAATGGATCA 
GATGACCCTG AAGATGCAGG AGCTGGTGAA AATAGGAGAG TCAGTGGGAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGACCTCCAA GACCTTCACG ACCACCACCA 
CCCACCCCAC GTAGACCAGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGGTCTAGTA CAGGCTCTCT GCCGCCGACA AATACAAATA CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTAACTC AAGCTCCCTT GCCACCTGGT TGGGAGCAGA GAGTGGACCA GCACGGGCGA 
GTTTACTATG TAGATCATGT TGAGAAAAGA ACAACATGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCCGTCC GGAACTATGA ACAATGGCAG 
CTACAGCGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT CATTTATGGG 
AATCAAGATT TATTTGCTAC ATCACAAAGT AAAGAATTTG ATCCTCTTGG TCCATTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGCAGAGTAT ATTTCGTCAA CCACAACACA 
CGAATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGTTGGG AAATGAGATT CACAGTGGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACTACCA CCTATATAGA TCCCCGCACA GGAAAATCTG CCCTAGACAA TGGACCTCAG 
ATAGCCTATG TTCGGGACTT CAAAGCAAAG GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAACAGA TAATGAGCTT CAGTCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAAGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGATTTA TTGCCATGGC TCTGTTCCAT GGGAAATTCA TAGACACGGG TTTTTCTTTA 
CCATTCTATA AGCGTATCTT GAACAAACCA GTTGGACTCA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAGGAAAACA ATATTGAGGA ATGTGATTTG 
GAAATGTACT TCTCCGTTGA CAAAGAAATT CTAGGTGAAA TTAAGAGTCA TGATCTGAAA 
CCTAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATACAT CAGAATGGTA 
GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA GAACAGACAC AAGCTTTCTT TGAAGGCTTT 
AATGAAATTC TTCCCCAGCA ATATTTGCAA TACTTTGATG CAAAGGAATT AGAGGTCCTT 
TTATGTGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TATGCAAGGA CCAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTAGGAGGA 
TTTGCTGATC TCATGGGGAG CAATGGACCA CAGAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGC TACCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCATACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTG TTTGCCATAG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_113671.3

Translation

MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_031483.5
Organism	Homo sapiens(Human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031483.5

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCTCAGAATG ATGATGGCTC CAGATCCAAG GATGAAACAA GAGTGAGCAC AAATGGATCA 
GATGACCCTG AAGATGCAGG AGCTGGTGAA AATAGGAGAG TCAGTGGGAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGACCTCCAA GACCTTCACG ACCACCACCA 
CCCACCCCAC GTAGACCAGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGGTCTAGTA CAGGCTCTCT GCCGCCGACA AATACAAATA CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTAACTC AAGCTCCCTT GCCACCTGGT TGGGAGCAGA GAGTGGACCA GCACGGGCGA 
GTTTACTATG TAGATCATGT TGAGAAAAGA ACAACATGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCCGTCC GGAACTATGA ACAATGGCAG 
CTACAGCGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT CATTTATGGG 
AATCAAGATT TATTTGCTAC ATCACAAAGT AAAGAATTTG ATCCTCTTGG TCCATTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGCAGAGTAT ATTTCGTCAA CCACAACACA 
CGAATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGTTGGG AAATGAGATT CACAGTGGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACTACCA CCTATATAGA TCCCCGCACA GGAAAATCTG CCCTAGACAA TGGACCTCAG 
ATAGCCTATG TTCGGGACTT CAAAGCAAAG GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAACAGA TAATGAGCTT CAGTCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAAGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGATTTA TTGCCATGGC TCTGTTCCAT GGGAAATTCA TAGACACGGG TTTTTCTTTA 
CCATTCTATA AGCGTATCTT GAACAAACCA GTTGGACTCA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAGGAAAACA ATATTGAGGA ATGTGATTTG 
GAAATGTACT TCTCCGTTGA CAAAGAAATT CTAGGTGAAA TTAAGAGTCA TGATCTGAAA 
CCTAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATACAT CAGAATGGTA 
GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA GAACAGACAC AAGCTTTCTT TGAAGGCTTT 
AATGAAATTC TTCCCCAGCA ATATTTGCAA TACTTTGATG CAAAGGAATT AGAGGTCCTT 
TTATGTGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TATGCAAGGA CCAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTAGGAGGA 
TTTGCTGATC TCATGGGGAG CAATGGACCA CAGAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGC TACCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCATACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTG TTTGCCATAG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( NM_031483.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_113671.3 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22317
Clone ID Related Accession (Same CDS sequence)	NM_001324198.2 , NM_031483.6 , NM_001324198.1 , NM_031483.5 , NM_031483.7
Accession Version	NM_031483.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL705688.1, AK315212.1, AL109923.29, AA730741.1 and BG928040.1. On Jun 2, 2019 this sequence version replaced NM_031483.6. Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Both variants 2 and 5 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.210367.1, SRR1803611.130987.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCTCAGAATG ATGATGGCTC CAGATCCAAG GATGAAACAA GAGTGAGCAC AAATGGATCA 
GATGACCCTG AAGATGCAGG AGCTGGTGAA AATAGGAGAG TCAGTGGGAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGACCTCCAA GACCTTCACG ACCACCACCA 
CCCACCCCAC GTAGACCAGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGGTCTAGTA CAGGCTCTCT GCCGCCGACA AATACAAATA CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTAACTC AAGCTCCCTT GCCACCTGGT TGGGAGCAGA GAGTGGACCA GCACGGGCGA 
GTTTACTATG TAGATCATGT TGAGAAAAGA ACAACATGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCCGTCC GGAACTATGA ACAATGGCAG 
CTACAGCGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT CATTTATGGG 
AATCAAGATT TATTTGCTAC ATCACAAAGT AAAGAATTTG ATCCTCTTGG TCCATTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGCAGAGTAT ATTTCGTCAA CCACAACACA 
CGAATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGTTGGG AAATGAGATT CACAGTGGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACTACCA CCTATATAGA TCCCCGCACA GGAAAATCTG CCCTAGACAA TGGACCTCAG 
ATAGCCTATG TTCGGGACTT CAAAGCAAAG GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAACAGA TAATGAGCTT CAGTCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAAGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGATTTA TTGCCATGGC TCTGTTCCAT GGGAAATTCA TAGACACGGG TTTTTCTTTA 
CCATTCTATA AGCGTATCTT GAACAAACCA GTTGGACTCA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAGGAAAACA ATATTGAGGA ATGTGATTTG 
GAAATGTACT TCTCCGTTGA CAAAGAAATT CTAGGTGAAA TTAAGAGTCA TGATCTGAAA 
CCTAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATACAT CAGAATGGTA 
GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA GAACAGACAC AAGCTTTCTT TGAAGGCTTT 
AATGAAATTC TTCCCCAGCA ATATTTGCAA TACTTTGATG CAAAGGAATT AGAGGTCCTT 
TTATGTGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TATGCAAGGA CCAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTAGGAGGA 
TTTGCTGATC TCATGGGGAG CAATGGACCA CAGAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGC TACCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCATACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTG TTTGCCATAG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113671.3
CDS	176..2764
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_031483.7
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031483.7

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCTCAGAATG ATGATGGCTC CAGATCCAAG GATGAAACAA GAGTGAGCAC AAATGGATCA 
GATGACCCTG AAGATGCAGG AGCTGGTGAA AATAGGAGAG TCAGTGGGAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGACCTCCAA GACCTTCACG ACCACCACCA 
CCCACCCCAC GTAGACCAGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGGTCTAGTA CAGGCTCTCT GCCGCCGACA AATACAAATA CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTAACTC AAGCTCCCTT GCCACCTGGT TGGGAGCAGA GAGTGGACCA GCACGGGCGA 
GTTTACTATG TAGATCATGT TGAGAAAAGA ACAACATGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCCGTCC GGAACTATGA ACAATGGCAG 
CTACAGCGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT CATTTATGGG 
AATCAAGATT TATTTGCTAC ATCACAAAGT AAAGAATTTG ATCCTCTTGG TCCATTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGCAGAGTAT ATTTCGTCAA CCACAACACA 
CGAATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGTTGGG AAATGAGATT CACAGTGGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACTACCA CCTATATAGA TCCCCGCACA GGAAAATCTG CCCTAGACAA TGGACCTCAG 
ATAGCCTATG TTCGGGACTT CAAAGCAAAG GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAACAGA TAATGAGCTT CAGTCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAAGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGATTTA TTGCCATGGC TCTGTTCCAT GGGAAATTCA TAGACACGGG TTTTTCTTTA 
CCATTCTATA AGCGTATCTT GAACAAACCA GTTGGACTCA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAGGAAAACA ATATTGAGGA ATGTGATTTG 
GAAATGTACT TCTCCGTTGA CAAAGAAATT CTAGGTGAAA TTAAGAGTCA TGATCTGAAA 
CCTAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATACAT CAGAATGGTA 
GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA GAACAGACAC AAGCTTTCTT TGAAGGCTTT 
AATGAAATTC TTCCCCAGCA ATATTTGCAA TACTTTGATG CAAAGGAATT AGAGGTCCTT 
TTATGTGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TATGCAAGGA CCAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTAGGAGGA 
TTTGCTGATC TCATGGGGAG CAATGGACCA CAGAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGC TACCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCATACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTG TTTGCCATAG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( NM_001257137.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244066.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22837
Clone ID Related Accession (Same CDS sequence)	XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , XM_024452005.1 , NM_001257137.3 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1
Accession Version	NM_001257137.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-02
Organism	Homo sapiens(Human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform 1
Comment

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_001244066.1

Translation

MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTGASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001257137.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257137.1

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( NM_001324197.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311126.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22837
Clone ID Related Accession (Same CDS sequence)	XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , XM_024452005.1 , NM_001257137.3 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1
Accession Version	NM_001324197.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL705688.1, AL356299.16, AL109923.29 and BG928040.1. On Apr 28, 2016 this sequence version replaced XM_011529077.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.88496.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311126.1
CDS	235..2946
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTGASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001324197.1
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324197.1

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( NM_001257137.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244066.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22837
Clone ID Related Accession (Same CDS sequence)	XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , XM_024452005.1 , NM_001257137.3 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1
Accession Version	NM_001257137.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL705688.1, BC011571.1, AL109923.29, AA730741.1 and BG928040.1. On Jun 2, 2019 this sequence version replaced NM_001257137.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244066.1
CDS	176..2887
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTGASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001257137.3
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257137.3

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( NM_001324197.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311126.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22837
Clone ID Related Accession (Same CDS sequence)	XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , XM_024452005.1 , NM_001257137.3 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1
Accession Version	NM_001324197.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL109923.29 and BG928040.1. On Jun 2, 2019 this sequence version replaced NM_001324197.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.88496.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311126.1
CDS	197..2908
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTGASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001324197.2
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324197.2

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( NM_001257137.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244066.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22837
Clone ID Related Accession (Same CDS sequence)	XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , XM_024452005.1 , NM_001257137.3 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1
Accession Version	NM_001257137.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL705688.1, BC011571.1, AL109923.29, AA730741.1 and BG928040.1. On Apr 28, 2016 this sequence version replaced NM_001257137.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244066.1
CDS	214..2925
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTGASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001257137.2
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257137.2

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( NM_001257138.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244067.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu23120
Clone ID Related Accession (Same CDS sequence)	NM_001257138.2 , NM_001257138.1 , XM_017028091.1 , NM_001257138.3
Accession Version	NM_001257138.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL705688.1, AK304090.1, AF038564.1, AL109923.29, AA730741.1 and BG928040.1. On Apr 28, 2016 this sequence version replaced NM_001257138.1. Transcript Variant: This variant (3) lacks two internal exons and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG 
ACAATAGGAG ACTTGTCAAT TTGTCTTGAT GGGCTACAGT TAGAGTCTGA AGTTGTTACC 
AATGGTGAAA CTACATGTTC AGAAAGTGCT TCTCAGAATG ATGATGGCTC CAGATCCAAG 
GATGAAACAA GAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA 
AATAGGAGAG TCAGTGGGAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGACCTCCAA GACCTTCACG ACCACCACCA CCCACCCCAC GTAGACCAGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGGTCTAGTA CAGGCTCTCT GCCGCCGACA 
AATACAAATA CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTAACTC AAGCTCCCTT GCCACCTGGT 
TGGGAGCAGA GAGTGGACCA GCACGGGCGA GTTTACTATG TAGATCATGT TGAGAAAAGA 
ACAACATGGG ATAGACCAGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ATTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCAACACTG 
GAATCCGTCC GGAACTATGA ACAATGGCAG CTACAGCGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT CATTTATGGG AATCAAGATT TATTTGCTAC ATCACAAAGT 
AAAGAATTTG ATCCTCTTGG TCCATTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGCAGAGTAT ATTTCGTCAA CCACAACACA CGAATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAAT TAAATGAAAA GCCCTTACCT GAAGGTTGGG AAATGAGATT CACAGTGGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACTACCA CCTATATAGA TCCCCGCACA 
GGAAAATCTG CCCTAGACAA TGGACCTCAG ATAGCCTATG TTCGGGACTT CAAAGCAAAG 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACATTGTT TGAGGATTCC TTTCAACAGA TAATGAGCTT CAGTCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AATGGTTCTT TCTTTTGTCA CATGAAGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATAA ACCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGATTTA TTGCCATGGC TCTGTTCCAT 
GGGAAATTCA TAGACACGGG TTTTTCTTTA CCATTCTATA AGCGTATCTT GAACAAACCA 
GTTGGACTCA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAGGAAAACA ATATTGAGGA ATGTGATTTG GAAATGTACT TCTCCGTTGA CAAAGAAATT 
CTAGGTGAAA TTAAGAGTCA TGATCTGAAA CCTAATGGTG GCAATATTCT TGTAACAGAA 
GAAAATAAAG AGGAATACAT CAGAATGGTA GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA 
GAACAGACAC AAGCTTTCTT TGAAGGCTTT AATGAAATTC TTCCCCAGCA ATATTTGCAA 
TACTTTGATG CAAAGGAATT AGAGGTCCTT TTATGTGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TATGCAAGGA CCAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTAGGAGGA TTTGCTGATC TCATGGGGAG CAATGGACCA 
CAGAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGC TACCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCATACAAG AGCTATGAGC AACTGAAGGA AAAGCTGTTG 
TTTGCCATAG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244067.1
CDS	402..2660
Translation	MKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001257138.2
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257138.2

ORF Insert Sequence:

ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG 
ACAATAGGAG ACTTGTCAAT TTGTCTTGAT GGGCTACAGT TAGAGTCTGA AGTTGTTACC 
AATGGTGAAA CTACATGTTC AGAAAGTGCT TCTCAGAATG ATGATGGCTC CAGATCCAAG 
GATGAAACAA GAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA 
AATAGGAGAG TCAGTGGGAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGACCTCCAA GACCTTCACG ACCACCACCA CCCACCCCAC GTAGACCAGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGGTCTAGTA CAGGCTCTCT GCCGCCGACA 
AATACAAATA CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTAACTC AAGCTCCCTT GCCACCTGGT 
TGGGAGCAGA GAGTGGACCA GCACGGGCGA GTTTACTATG TAGATCATGT TGAGAAAAGA 
ACAACATGGG ATAGACCAGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ATTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCAACACTG 
GAATCCGTCC GGAACTATGA ACAATGGCAG CTACAGCGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT CATTTATGGG AATCAAGATT TATTTGCTAC ATCACAAAGT 
AAAGAATTTG ATCCTCTTGG TCCATTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGCAGAGTAT ATTTCGTCAA CCACAACACA CGAATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAAT TAAATGAAAA GCCCTTACCT GAAGGTTGGG AAATGAGATT CACAGTGGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACTACCA CCTATATAGA TCCCCGCACA 
GGAAAATCTG CCCTAGACAA TGGACCTCAG ATAGCCTATG TTCGGGACTT CAAAGCAAAG 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACATTGTT TGAGGATTCC TTTCAACAGA TAATGAGCTT CAGTCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AATGGTTCTT TCTTTTGTCA CATGAAGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATAA ACCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGATTTA TTGCCATGGC TCTGTTCCAT 
GGGAAATTCA TAGACACGGG TTTTTCTTTA CCATTCTATA AGCGTATCTT GAACAAACCA 
GTTGGACTCA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAGGAAAACA ATATTGAGGA ATGTGATTTG GAAATGTACT TCTCCGTTGA CAAAGAAATT 
CTAGGTGAAA TTAAGAGTCA TGATCTGAAA CCTAATGGTG GCAATATTCT TGTAACAGAA 
GAAAATAAAG AGGAATACAT CAGAATGGTA GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA 
GAACAGACAC AAGCTTTCTT TGAAGGCTTT AATGAAATTC TTCCCCAGCA ATATTTGCAA 
TACTTTGATG CAAAGGAATT AGAGGTCCTT TTATGTGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TATGCAAGGA CCAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTAGGAGGA TTTGCTGATC TCATGGGGAG CAATGGACCA 
CAGAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGC TACCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCATACAAG AGCTATGAGC AACTGAAGGA AAAGCTGTTG 
TTTGCCATAG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( NM_001257138.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244067.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu23120
Clone ID Related Accession (Same CDS sequence)	NM_001257138.2 , NM_001257138.1 , XM_017028091.1 , NM_001257138.3
Accession Version	NM_001257138.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-02
Organism	Homo sapiens(Human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform 3
Comment

ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG 
ACAATAGGAG ACTTGTCAAT TTGTCTTGAT GGGCTACAGT TAGAGTCTGA AGTTGTTACC 
AATGGTGAAA CTACATGTTC AGAAAGTGCT TCTCAGAATG ATGATGGCTC CAGATCCAAG 
GATGAAACAA GAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA 
AATAGGAGAG TCAGTGGGAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGACCTCCAA GACCTTCACG ACCACCACCA CCCACCCCAC GTAGACCAGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGGTCTAGTA CAGGCTCTCT GCCGCCGACA 
AATACAAATA CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTAACTC AAGCTCCCTT GCCACCTGGT 
TGGGAGCAGA GAGTGGACCA GCACGGGCGA GTTTACTATG TAGATCATGT TGAGAAAAGA 
ACAACATGGG ATAGACCAGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ATTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCAACACTG 
GAATCCGTCC GGAACTATGA ACAATGGCAG CTACAGCGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT CATTTATGGG AATCAAGATT TATTTGCTAC ATCACAAAGT 
AAAGAATTTG ATCCTCTTGG TCCATTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGCAGAGTAT ATTTCGTCAA CCACAACACA CGAATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAAT TAAATGAAAA GCCCTTACCT GAAGGTTGGG AAATGAGATT CACAGTGGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACTACCA CCTATATAGA TCCCCGCACA 
GGAAAATCTG CCCTAGACAA TGGACCTCAG ATAGCCTATG TTCGGGACTT CAAAGCAAAG 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACATTGTT TGAGGATTCC TTTCAACAGA TAATGAGCTT CAGTCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AATGGTTCTT TCTTTTGTCA CATGAAGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATAA ACCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGATTTA TTGCCATGGC TCTGTTCCAT 
GGGAAATTCA TAGACACGGG TTTTTCTTTA CCATTCTATA AGCGTATCTT GAACAAACCA 
GTTGGACTCA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAGGAAAACA ATATTGAGGA ATGTGATTTG GAAATGTACT TCTCCGTTGA CAAAGAAATT 
CTAGGTGAAA TTAAGAGTCA TGATCTGAAA CCTAATGGTG GCAATATTCT TGTAACAGAA 
GAAAATAAAG AGGAATACAT CAGAATGGTA GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA 
GAACAGACAC AAGCTTTCTT TGAAGGCTTT AATGAAATTC TTCCCCAGCA ATATTTGCAA 
TACTTTGATG CAAAGGAATT AGAGGTCCTT TTATGTGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TATGCAAGGA CCAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTAGGAGGA TTTGCTGATC TCATGGGGAG CAATGGACCA 
CAGAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGC TACCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCATACAAG AGCTATGAGC AACTGAAGGA AAAGCTGTTG 
TTTGCCATAG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_001244067.1

Translation

MKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001257138.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257138.1

ORF Insert Sequence:

ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG 
ACAATAGGAG ACTTGTCAAT TTGTCTTGAT GGGCTACAGT TAGAGTCTGA AGTTGTTACC 
AATGGTGAAA CTACATGTTC AGAAAGTGCT TCTCAGAATG ATGATGGCTC CAGATCCAAG 
GATGAAACAA GAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA 
AATAGGAGAG TCAGTGGGAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGACCTCCAA GACCTTCACG ACCACCACCA CCCACCCCAC GTAGACCAGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGGTCTAGTA CAGGCTCTCT GCCGCCGACA 
AATACAAATA CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTAACTC AAGCTCCCTT GCCACCTGGT 
TGGGAGCAGA GAGTGGACCA GCACGGGCGA GTTTACTATG TAGATCATGT TGAGAAAAGA 
ACAACATGGG ATAGACCAGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ATTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCAACACTG 
GAATCCGTCC GGAACTATGA ACAATGGCAG CTACAGCGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT CATTTATGGG AATCAAGATT TATTTGCTAC ATCACAAAGT 
AAAGAATTTG ATCCTCTTGG TCCATTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGCAGAGTAT ATTTCGTCAA CCACAACACA CGAATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAAT TAAATGAAAA GCCCTTACCT GAAGGTTGGG AAATGAGATT CACAGTGGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACTACCA CCTATATAGA TCCCCGCACA 
GGAAAATCTG CCCTAGACAA TGGACCTCAG ATAGCCTATG TTCGGGACTT CAAAGCAAAG 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACATTGTT TGAGGATTCC TTTCAACAGA TAATGAGCTT CAGTCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AATGGTTCTT TCTTTTGTCA CATGAAGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATAA ACCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGATTTA TTGCCATGGC TCTGTTCCAT 
GGGAAATTCA TAGACACGGG TTTTTCTTTA CCATTCTATA AGCGTATCTT GAACAAACCA 
GTTGGACTCA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAGGAAAACA ATATTGAGGA ATGTGATTTG GAAATGTACT TCTCCGTTGA CAAAGAAATT 
CTAGGTGAAA TTAAGAGTCA TGATCTGAAA CCTAATGGTG GCAATATTCT TGTAACAGAA 
GAAAATAAAG AGGAATACAT CAGAATGGTA GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA 
GAACAGACAC AAGCTTTCTT TGAAGGCTTT AATGAAATTC TTCCCCAGCA ATATTTGCAA 
TACTTTGATG CAAAGGAATT AGAGGTCCTT TTATGTGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TATGCAAGGA CCAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTAGGAGGA TTTGCTGATC TCATGGGGAG CAATGGACCA 
CAGAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGC TACCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCATACAAG AGCTATGAGC AACTGAAGGA AAAGCTGTTG 
TTTGCCATAG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( NM_001257138.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244067.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 3, mRNA.

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CloneID	OHu23120
Clone ID Related Accession (Same CDS sequence)	NM_001257138.2 , NM_001257138.1 , XM_017028091.1 , NM_001257138.3
Accession Version	NM_001257138.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL705688.1, AK304090.1, AF038564.1, AL109923.29, AA730741.1 and BG928040.1. On Jun 2, 2019 this sequence version replaced NM_001257138.2. Transcript Variant: This variant (3) lacks two internal exons and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG 
ACAATAGGAG ACTTGTCAAT TTGTCTTGAT GGGCTACAGT TAGAGTCTGA AGTTGTTACC 
AATGGTGAAA CTACATGTTC AGAAAGTGCT TCTCAGAATG ATGATGGCTC CAGATCCAAG 
GATGAAACAA GAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA 
AATAGGAGAG TCAGTGGGAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGACCTCCAA GACCTTCACG ACCACCACCA CCCACCCCAC GTAGACCAGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGGTCTAGTA CAGGCTCTCT GCCGCCGACA 
AATACAAATA CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTAACTC AAGCTCCCTT GCCACCTGGT 
TGGGAGCAGA GAGTGGACCA GCACGGGCGA GTTTACTATG TAGATCATGT TGAGAAAAGA 
ACAACATGGG ATAGACCAGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ATTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCAACACTG 
GAATCCGTCC GGAACTATGA ACAATGGCAG CTACAGCGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT CATTTATGGG AATCAAGATT TATTTGCTAC ATCACAAAGT 
AAAGAATTTG ATCCTCTTGG TCCATTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGCAGAGTAT ATTTCGTCAA CCACAACACA CGAATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAAT TAAATGAAAA GCCCTTACCT GAAGGTTGGG AAATGAGATT CACAGTGGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACTACCA CCTATATAGA TCCCCGCACA 
GGAAAATCTG CCCTAGACAA TGGACCTCAG ATAGCCTATG TTCGGGACTT CAAAGCAAAG 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACATTGTT TGAGGATTCC TTTCAACAGA TAATGAGCTT CAGTCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AATGGTTCTT TCTTTTGTCA CATGAAGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATAA ACCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGATTTA TTGCCATGGC TCTGTTCCAT 
GGGAAATTCA TAGACACGGG TTTTTCTTTA CCATTCTATA AGCGTATCTT GAACAAACCA 
GTTGGACTCA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAGGAAAACA ATATTGAGGA ATGTGATTTG GAAATGTACT TCTCCGTTGA CAAAGAAATT 
CTAGGTGAAA TTAAGAGTCA TGATCTGAAA CCTAATGGTG GCAATATTCT TGTAACAGAA 
GAAAATAAAG AGGAATACAT CAGAATGGTA GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA 
GAACAGACAC AAGCTTTCTT TGAAGGCTTT AATGAAATTC TTCCCCAGCA ATATTTGCAA 
TACTTTGATG CAAAGGAATT AGAGGTCCTT TTATGTGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TATGCAAGGA CCAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTAGGAGGA TTTGCTGATC TCATGGGGAG CAATGGACCA 
CAGAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGC TACCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCATACAAG AGCTATGAGC AACTGAAGGA AAAGCTGTTG 
TTTGCCATAG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244067.1
CDS	364..2622
Translation	MKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001257138.3
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257138.3

ORF Insert Sequence:

ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG 
ACAATAGGAG ACTTGTCAAT TTGTCTTGAT GGGCTACAGT TAGAGTCTGA AGTTGTTACC 
AATGGTGAAA CTACATGTTC AGAAAGTGCT TCTCAGAATG ATGATGGCTC CAGATCCAAG 
GATGAAACAA GAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA 
AATAGGAGAG TCAGTGGGAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGACCTCCAA GACCTTCACG ACCACCACCA CCCACCCCAC GTAGACCAGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGGTCTAGTA CAGGCTCTCT GCCGCCGACA 
AATACAAATA CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTAACTC AAGCTCCCTT GCCACCTGGT 
TGGGAGCAGA GAGTGGACCA GCACGGGCGA GTTTACTATG TAGATCATGT TGAGAAAAGA 
ACAACATGGG ATAGACCAGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ATTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCAACACTG 
GAATCCGTCC GGAACTATGA ACAATGGCAG CTACAGCGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT CATTTATGGG AATCAAGATT TATTTGCTAC ATCACAAAGT 
AAAGAATTTG ATCCTCTTGG TCCATTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGCAGAGTAT ATTTCGTCAA CCACAACACA CGAATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAAT TAAATGAAAA GCCCTTACCT GAAGGTTGGG AAATGAGATT CACAGTGGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACTACCA CCTATATAGA TCCCCGCACA 
GGAAAATCTG CCCTAGACAA TGGACCTCAG ATAGCCTATG TTCGGGACTT CAAAGCAAAG 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACATTGTT TGAGGATTCC TTTCAACAGA TAATGAGCTT CAGTCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AATGGTTCTT TCTTTTGTCA CATGAAGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATAA ACCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGATTTA TTGCCATGGC TCTGTTCCAT 
GGGAAATTCA TAGACACGGG TTTTTCTTTA CCATTCTATA AGCGTATCTT GAACAAACCA 
GTTGGACTCA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAGGAAAACA ATATTGAGGA ATGTGATTTG GAAATGTACT TCTCCGTTGA CAAAGAAATT 
CTAGGTGAAA TTAAGAGTCA TGATCTGAAA CCTAATGGTG GCAATATTCT TGTAACAGAA 
GAAAATAAAG AGGAATACAT CAGAATGGTA GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA 
GAACAGACAC AAGCTTTCTT TGAAGGCTTT AATGAAATTC TTCCCCAGCA ATATTTGCAA 
TACTTTGATG CAAAGGAATT AGAGGTCCTT TTATGTGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TATGCAAGGA CCAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTAGGAGGA TTTGCTGATC TCATGGGGAG CAATGGACCA 
CAGAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGC TACCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCATACAAG AGCTATGAGC AACTGAAGGA AAAGCTGTTG 
TTTGCCATAG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_011529078.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527380.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.

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CloneID	OHu22837
Clone ID Related Accession (Same CDS sequence)	XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , XM_024452005.1 , NM_001257137.3 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1
Accession Version	XM_011529078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011527380.1

Translation

MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTGASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_011529078.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529078.1

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_024452005.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307773.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu22837
Clone ID Related Accession (Same CDS sequence)	XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , XM_024452005.1 , NM_001257137.3 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1
Accession Version	XM_024452005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307773.1
CDS	46..2757
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTGASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_024452005.1
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452005.1

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_011529077.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527379.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu22837
Clone ID Related Accession (Same CDS sequence)	XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , XM_024452005.1 , NM_001257137.3 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1
Accession Version	XM_011529077.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011527379.1

Translation

MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTGASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_011529077.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529077.1

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_017028089.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883578.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu22837
Clone ID Related Accession (Same CDS sequence)	XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , XM_024452005.1 , NM_001257137.3 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1
Accession Version	XM_017028089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883578.1
CDS	47..2758
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTGASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_017028089.1
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028089.1

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG TCATCTCAGC AAAACTTAAG GAAAATAAGA AGAATTGGTT TGGACCAAGT 
CCTTACGTAG AGGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAACACA 
AACAGTCCCA AGTGGAAGCA ACCCCTTACA GTTATCGTTA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AAGAAGTAGT TGTGACTTTG 
CAGCTTGGAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCAAT TTGTCTTGAT 
GGGCTACAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAATGGG 
GTTTCGCTCT GTTTGCCCAG GCTGGAGTGC AATAGTGCGA TCTCGGCTCA CTGCAACCTC 
TGCCTCCCGG GTTTAAGTGA TTCTCCTATC TCAGCCTCCC GAGTAGCTGG GTTTACAGGT 
GCTTCTCAGA ATGATGATGG CTCCAGATCC AAGGATGAAA CAAGAGTGAG CACAAATGGA 
TCAGATGACC CTGAAGATGC AGGAGCTGGT GAAAATAGGA GAGTCAGTGG GAATAATTCT 
CCATCACTCT CAAATGGTGG TTTTAAACCT TCTAGACCTC CAAGACCTTC ACGACCACCA 
CCACCCACCC CACGTAGACC AGCATCTGTC AATGGTTCAC CATCTGCCAC TTCTGAAAGT 
GATGGGTCTA GTACAGGCTC TCTGCCGCCG ACAAATACAA ATACAAATAC ATCTGAAGGA 
GCAACATCTG GATTAATAAT TCCTCTTACT ATATCTGGAG GCTCAGGCCC TAGGCCATTA 
AATCCTGTAA CTCAAGCTCC CTTGCCACCT GGTTGGGAGC AGAGAGTGGA CCAGCACGGG 
CGAGTTTACT ATGTAGATCA TGTTGAGAAA AGAACAACAT GGGATAGACC AGAACCTCTA 
CCTCCTGGCT GGGAACGGCG GGTTGACAAC ATGGGACGTA TTTATTATGT TGACCATTTC 
ACAAGAACAA CAACGTGGCA GAGGCCAACA CTGGAATCCG TCCGGAACTA TGAACAATGG 
CAGCTACAGC GTAGTCAGCT TCAAGGAGCA ATGCAGCAGT TTAACCAGAG ATTCATTTAT 
GGGAATCAAG ATTTATTTGC TACATCACAA AGTAAAGAAT TTGATCCTCT TGGTCCATTG 
CCACCTGGAT GGGAGAAGAG AACAGACAGC AATGGCAGAG TATATTTCGT CAACCACAAC 
ACACGAATTA CACAATGGGA AGACCCCAGA AGTCAAGGTC AATTAAATGA AAAGCCCTTA 
CCTGAAGGTT GGGAAATGAG ATTCACAGTG GATGGAATTC CATATTTTGT GGACCACAAT 
AGAAGAACTA CCACCTATAT AGATCCCCGC ACAGGAAAAT CTGCCCTAGA CAATGGACCT 
CAGATAGCCT ATGTTCGGGA CTTCAAAGCA AAGGTTCAGT ATTTCCGGTT CTGGTGTCAG 
CAACTGGCCA TGCCACAGCA CATAAAGATT ACAGTGACAA GAAAAACATT GTTTGAGGAT 
TCCTTTCAAC AGATAATGAG CTTCAGTCCC CAAGATCTGC GAAGACGTTT GTGGGTGATT 
TTTCCAGGAG AAGAAGGTTT AGATTATGGA GGTGTAGCAA GAGAATGGTT CTTTCTTTTG 
TCACATGAAG TGTTGAACCC AATGTATTGC CTGTTTGAAT ATGCAGGGAA GGATAACTAC 
TGCTTGCAGA TAAACCCCGC TTCTTACATC AATCCAGATC ACCTGAAATA TTTTCGTTTT 
ATTGGCAGAT TTATTGCCAT GGCTCTGTTC CATGGGAAAT TCATAGACAC GGGTTTTTCT 
TTACCATTCT ATAAGCGTAT CTTGAACAAA CCAGTTGGAC TCAAGGATTT AGAATCTATT 
GATCCAGAAT TTTACAATTC TCTCATCTGG GTTAAGGAAA ACAATATTGA GGAATGTGAT 
TTGGAAATGT ACTTCTCCGT TGACAAAGAA ATTCTAGGTG AAATTAAGAG TCATGATCTG 
AAACCTAATG GTGGCAATAT TCTTGTAACA GAAGAAAATA AAGAGGAATA CATCAGAATG 
GTAGCTGAGT GGAGGTTGTC TCGAGGTGTT GAAGAACAGA CACAAGCTTT CTTTGAAGGC 
TTTAATGAAA TTCTTCCCCA GCAATATTTG CAATACTTTG ATGCAAAGGA ATTAGAGGTC 
CTTTTATGTG GAATGCAAGA GATTGATTTG AATGACTGGC AAAGACATGC CATCTACCGT 
CATTATGCAA GGACCAGCAA ACAAATCATG TGGTTTTGGC AGTTTGTTAA AGAAATTGAT 
AATGAGAAGA GAATGAGACT TCTGCAGTTT GTTACTGGAA CCTGCCGATT GCCAGTAGGA 
GGATTTGCTG ATCTCATGGG GAGCAATGGA CCACAGAAAT TCTGCATTGA AAAAGTTGGG 
AAAGAAAATT GGCTACCCAG AAGTCATACC TGTTTTAATC GCCTGGACCT GCCACCATAC 
AAGAGCTATG AGCAACTGAA GGAAAAGCTG TTGTTTGCCA TAGAAGAAAC AGAAGGATTT 
GGACAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_017028091.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883580.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu23120
Clone ID Related Accession (Same CDS sequence)	NM_001257138.2 , NM_001257138.1 , XM_017028091.1 , NM_001257138.3
Accession Version	XM_017028091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG 
ACAATAGGAG ACTTGTCAAT TTGTCTTGAT GGGCTACAGT TAGAGTCTGA AGTTGTTACC 
AATGGTGAAA CTACATGTTC AGAAAGTGCT TCTCAGAATG ATGATGGCTC CAGATCCAAG 
GATGAAACAA GAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA 
AATAGGAGAG TCAGTGGGAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGACCTCCAA GACCTTCACG ACCACCACCA CCCACCCCAC GTAGACCAGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGGTCTAGTA CAGGCTCTCT GCCGCCGACA 
AATACAAATA CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTAACTC AAGCTCCCTT GCCACCTGGT 
TGGGAGCAGA GAGTGGACCA GCACGGGCGA GTTTACTATG TAGATCATGT TGAGAAAAGA 
ACAACATGGG ATAGACCAGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ATTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCAACACTG 
GAATCCGTCC GGAACTATGA ACAATGGCAG CTACAGCGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT CATTTATGGG AATCAAGATT TATTTGCTAC ATCACAAAGT 
AAAGAATTTG ATCCTCTTGG TCCATTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGCAGAGTAT ATTTCGTCAA CCACAACACA CGAATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAAT TAAATGAAAA GCCCTTACCT GAAGGTTGGG AAATGAGATT CACAGTGGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACTACCA CCTATATAGA TCCCCGCACA 
GGAAAATCTG CCCTAGACAA TGGACCTCAG ATAGCCTATG TTCGGGACTT CAAAGCAAAG 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACATTGTT TGAGGATTCC TTTCAACAGA TAATGAGCTT CAGTCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AATGGTTCTT TCTTTTGTCA CATGAAGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATAA ACCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGATTTA TTGCCATGGC TCTGTTCCAT 
GGGAAATTCA TAGACACGGG TTTTTCTTTA CCATTCTATA AGCGTATCTT GAACAAACCA 
GTTGGACTCA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAGGAAAACA ATATTGAGGA ATGTGATTTG GAAATGTACT TCTCCGTTGA CAAAGAAATT 
CTAGGTGAAA TTAAGAGTCA TGATCTGAAA CCTAATGGTG GCAATATTCT TGTAACAGAA 
GAAAATAAAG AGGAATACAT CAGAATGGTA GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA 
GAACAGACAC AAGCTTTCTT TGAAGGCTTT AATGAAATTC TTCCCCAGCA ATATTTGCAA 
TACTTTGATG CAAAGGAATT AGAGGTCCTT TTATGTGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TATGCAAGGA CCAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTAGGAGGA TTTGCTGATC TCATGGGGAG CAATGGACCA 
CAGAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGC TACCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCATACAAG AGCTATGAGC AACTGAAGGA AAAGCTGTTG 
TTTGCCATAG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883580.1
CDS	415..2673
Translation	MKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_017028091.1
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028091.1

ORF Insert Sequence:

ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG 
ACAATAGGAG ACTTGTCAAT TTGTCTTGAT GGGCTACAGT TAGAGTCTGA AGTTGTTACC 
AATGGTGAAA CTACATGTTC AGAAAGTGCT TCTCAGAATG ATGATGGCTC CAGATCCAAG 
GATGAAACAA GAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA 
AATAGGAGAG TCAGTGGGAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGACCTCCAA GACCTTCACG ACCACCACCA CCCACCCCAC GTAGACCAGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGGTCTAGTA CAGGCTCTCT GCCGCCGACA 
AATACAAATA CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTAACTC AAGCTCCCTT GCCACCTGGT 
TGGGAGCAGA GAGTGGACCA GCACGGGCGA GTTTACTATG TAGATCATGT TGAGAAAAGA 
ACAACATGGG ATAGACCAGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ATTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCAACACTG 
GAATCCGTCC GGAACTATGA ACAATGGCAG CTACAGCGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT CATTTATGGG AATCAAGATT TATTTGCTAC ATCACAAAGT 
AAAGAATTTG ATCCTCTTGG TCCATTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGCAGAGTAT ATTTCGTCAA CCACAACACA CGAATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAAT TAAATGAAAA GCCCTTACCT GAAGGTTGGG AAATGAGATT CACAGTGGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACTACCA CCTATATAGA TCCCCGCACA 
GGAAAATCTG CCCTAGACAA TGGACCTCAG ATAGCCTATG TTCGGGACTT CAAAGCAAAG 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACATTGTT TGAGGATTCC TTTCAACAGA TAATGAGCTT CAGTCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AATGGTTCTT TCTTTTGTCA CATGAAGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATAA ACCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGATTTA TTGCCATGGC TCTGTTCCAT 
GGGAAATTCA TAGACACGGG TTTTTCTTTA CCATTCTATA AGCGTATCTT GAACAAACCA 
GTTGGACTCA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAGGAAAACA ATATTGAGGA ATGTGATTTG GAAATGTACT TCTCCGTTGA CAAAGAAATT 
CTAGGTGAAA TTAAGAGTCA TGATCTGAAA CCTAATGGTG GCAATATTCT TGTAACAGAA 
GAAAATAAAG AGGAATACAT CAGAATGGTA GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA 
GAACAGACAC AAGCTTTCTT TGAAGGCTTT AATGAAATTC TTCCCCAGCA ATATTTGCAA 
TACTTTGATG CAAAGGAATT AGAGGTCCTT TTATGTGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TATGCAAGGA CCAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTAGGAGGA TTTGCTGATC TCATGGGGAG CAATGGACCA 
CAGAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGC TACCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCATACAAG AGCTATGAGC AACTGAAGGA AAAGCTGTTG 
TTTGCCATAG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_017028092.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883581.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu93131
Clone ID Related Accession (Same CDS sequence)	XM_017028092.2 , XM_017028092.1
Accession Version	XM_017028092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG AGACGAGGTT TCACCATCTT GACCAGGCTG GCCTTGAACT CCTGACCTCA 
GGATCCACCC ACCTCGGCCT CCCAAAGTAC AGGGATTACA GGCGTGAGCC ACCGCGTCCG 
GCCTTCATCT CAGCAAAACT TAAGGAAAAT AAGAAGAATT GGTTTGGACC AAGTCCTTAC 
GTAGAGGTCA CAGTAGATGG ACAGTCAAAG AAGACAGAAA AATGCAACAA CACAAACAGT 
CCCAAGTGGA AGCAACCCCT TACAGTTATC GTTACCCCTG TGAGTAAATT ACATTTTCGT 
GTGTGGAGTC ACCAGACACT GAAATCTGAT GTTTTGTTGG GAACTGCTGC ATTAGATATT 
TATGAAACAT TAAAGTCAAA CAATATGAAA CTTGAAGAAG TAGTTGTGAC TTTGCAGCTT 
GGAGGTGACA AAGAGCCAAC AGAGACAATA GGAGACTTGT CAATTTGTCT TGATGGGCTA 
CAGTTAGAGT CTGAAGTTGT TACCAATGGT GAAACTACAT GTTCAGAAAG TGCTTCTCAG 
AATGATGATG GCTCCAGATC CAAGGATGAA ACAAGAGTGA GCACAAATGG ATCAGATGAC 
CCTGAAGATG CAGGAGCTGG TGAAAATAGG AGAGTCAGTG GGAATAATTC TCCATCACTC 
TCAAATGGTG GTTTTAAACC TTCTAGACCT CCAAGACCTT CACGACCACC ACCACCCACC 
CCACGTAGAC CAGCATCTGT CAATGGTTCA CCATCTGCCA CTTCTGAAAG TGATGGGTCT 
AGTACAGGCT CTCTGCCGCC GACAAATACA AATACAAATA CATCTGAAGG AGCAACATCT 
GGATTAATAA TTCCTCTTAC TATATCTGGA GGCTCAGGCC CTAGGCCATT AAATCCTGTA 
ACTCAAGCTC CCTTGCCACC TGGTTGGGAG CAGAGAGTGG ACCAGCACGG GCGAGTTTAC 
TATGTAGATC ATGTTGAGAA AAGAACAACA TGGGATAGAC CAGAACCTCT ACCTCCTGGC 
TGGGAACGGC GGGTTGACAA CATGGGACGT ATTTATTATG TTGACCATTT CACAAGAACA 
ACAACGTGGC AGAGGCCAAC ACTGGAATCC GTCCGGAACT ATGAACAATG GCAGCTACAG 
CGTAGTCAGC TTCAAGGAGC AATGCAGCAG TTTAACCAGA GATTCATTTA TGGGAATCAA 
GATTTATTTG CTACATCACA AAGTAAAGAA TTTGATCCTC TTGGTCCATT GCCACCTGGA 
TGGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883581.1
CDS	176..1504
Translation	MSDSGSQLGSMGSLTMKSQLQITETRFHHLDQAGLELLTSGSTHLGLPKYRDYRREPPRPAFISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWV

Target ORF information:

RefSeq Version	XM_017028092.2
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028092.2

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG AGACGAGGTT TCACCATCTT GACCAGGCTG GCCTTGAACT CCTGACCTCA 
GGATCCACCC ACCTCGGCCT CCCAAAGTAC AGGGATTACA GGCGTGAGCC ACCGCGTCCG 
GCCTTCATCT CAGCAAAACT TAAGGAAAAT AAGAAGAATT GGTTTGGACC AAGTCCTTAC 
GTAGAGGTCA CAGTAGATGG ACAGTCAAAG AAGACAGAAA AATGCAACAA CACAAACAGT 
CCCAAGTGGA AGCAACCCCT TACAGTTATC GTTACCCCTG TGAGTAAATT ACATTTTCGT 
GTGTGGAGTC ACCAGACACT GAAATCTGAT GTTTTGTTGG GAACTGCTGC ATTAGATATT 
TATGAAACAT TAAAGTCAAA CAATATGAAA CTTGAAGAAG TAGTTGTGAC TTTGCAGCTT 
GGAGGTGACA AAGAGCCAAC AGAGACAATA GGAGACTTGT CAATTTGTCT TGATGGGCTA 
CAGTTAGAGT CTGAAGTTGT TACCAATGGT GAAACTACAT GTTCAGAAAG TGCTTCTCAG 
AATGATGATG GCTCCAGATC CAAGGATGAA ACAAGAGTGA GCACAAATGG ATCAGATGAC 
CCTGAAGATG CAGGAGCTGG TGAAAATAGG AGAGTCAGTG GGAATAATTC TCCATCACTC 
TCAAATGGTG GTTTTAAACC TTCTAGACCT CCAAGACCTT CACGACCACC ACCACCCACC 
CCACGTAGAC CAGCATCTGT CAATGGTTCA CCATCTGCCA CTTCTGAAAG TGATGGGTCT 
AGTACAGGCT CTCTGCCGCC GACAAATACA AATACAAATA CATCTGAAGG AGCAACATCT 
GGATTAATAA TTCCTCTTAC TATATCTGGA GGCTCAGGCC CTAGGCCATT AAATCCTGTA 
ACTCAAGCTC CCTTGCCACC TGGTTGGGAG CAGAGAGTGG ACCAGCACGG GCGAGTTTAC 
TATGTAGATC ATGTTGAGAA AAGAACAACA TGGGATAGAC CAGAACCTCT ACCTCCTGGC 
TGGGAACGGC GGGTTGACAA CATGGGACGT ATTTATTATG TTGACCATTT CACAAGAACA 
ACAACGTGGC AGAGGCCAAC ACTGGAATCC GTCCGGAACT ATGAACAATG GCAGCTACAG 
CGTAGTCAGC TTCAAGGAGC AATGCAGCAG TTTAACCAGA GATTCATTTA TGGGAATCAA 
GATTTATTTG CTACATCACA AAGTAAAGAA TTTGATCCTC TTGGTCCATT GCCACCTGGA 
TGGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_017028092.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883581.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu93131
Clone ID Related Accession (Same CDS sequence)	XM_017028092.2 , XM_017028092.1
Accession Version	XM_017028092.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG AGACGAGGTT TCACCATCTT GACCAGGCTG GCCTTGAACT CCTGACCTCA 
GGATCCACCC ACCTCGGCCT CCCAAAGTAC AGGGATTACA GGCGTGAGCC ACCGCGTCCG 
GCCTTCATCT CAGCAAAACT TAAGGAAAAT AAGAAGAATT GGTTTGGACC AAGTCCTTAC 
GTAGAGGTCA CAGTAGATGG ACAGTCAAAG AAGACAGAAA AATGCAACAA CACAAACAGT 
CCCAAGTGGA AGCAACCCCT TACAGTTATC GTTACCCCTG TGAGTAAATT ACATTTTCGT 
GTGTGGAGTC ACCAGACACT GAAATCTGAT GTTTTGTTGG GAACTGCTGC ATTAGATATT 
TATGAAACAT TAAAGTCAAA CAATATGAAA CTTGAAGAAG TAGTTGTGAC TTTGCAGCTT 
GGAGGTGACA AAGAGCCAAC AGAGACAATA GGAGACTTGT CAATTTGTCT TGATGGGCTA 
CAGTTAGAGT CTGAAGTTGT TACCAATGGT GAAACTACAT GTTCAGAAAG TGCTTCTCAG 
AATGATGATG GCTCCAGATC CAAGGATGAA ACAAGAGTGA GCACAAATGG ATCAGATGAC 
CCTGAAGATG CAGGAGCTGG TGAAAATAGG AGAGTCAGTG GGAATAATTC TCCATCACTC 
TCAAATGGTG GTTTTAAACC TTCTAGACCT CCAAGACCTT CACGACCACC ACCACCCACC 
CCACGTAGAC CAGCATCTGT CAATGGTTCA CCATCTGCCA CTTCTGAAAG TGATGGGTCT 
AGTACAGGCT CTCTGCCGCC GACAAATACA AATACAAATA CATCTGAAGG AGCAACATCT 
GGATTAATAA TTCCTCTTAC TATATCTGGA GGCTCAGGCC CTAGGCCATT AAATCCTGTA 
ACTCAAGCTC CCTTGCCACC TGGTTGGGAG CAGAGAGTGG ACCAGCACGG GCGAGTTTAC 
TATGTAGATC ATGTTGAGAA AAGAACAACA TGGGATAGAC CAGAACCTCT ACCTCCTGGC 
TGGGAACGGC GGGTTGACAA CATGGGACGT ATTTATTATG TTGACCATTT CACAAGAACA 
ACAACGTGGC AGAGGCCAAC ACTGGAATCC GTCCGGAACT ATGAACAATG GCAGCTACAG 
CGTAGTCAGC TTCAAGGAGC AATGCAGCAG TTTAACCAGA GATTCATTTA TGGGAATCAA 
GATTTATTTG CTACATCACA AAGTAAAGAA TTTGATCCTC TTGGTCCATT GCCACCTGGA 
TGGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883581.1
CDS	176..1504
Translation	MSDSGSQLGSMGSLTMKSQLQITETRFHHLDQAGLELLTSGSTHLGLPKYRDYRREPPRPAFISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWV

Target ORF information:

RefSeq Version	XM_017028092.1
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028092.1

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG AGACGAGGTT TCACCATCTT GACCAGGCTG GCCTTGAACT CCTGACCTCA 
GGATCCACCC ACCTCGGCCT CCCAAAGTAC AGGGATTACA GGCGTGAGCC ACCGCGTCCG 
GCCTTCATCT CAGCAAAACT TAAGGAAAAT AAGAAGAATT GGTTTGGACC AAGTCCTTAC 
GTAGAGGTCA CAGTAGATGG ACAGTCAAAG AAGACAGAAA AATGCAACAA CACAAACAGT 
CCCAAGTGGA AGCAACCCCT TACAGTTATC GTTACCCCTG TGAGTAAATT ACATTTTCGT 
GTGTGGAGTC ACCAGACACT GAAATCTGAT GTTTTGTTGG GAACTGCTGC ATTAGATATT 
TATGAAACAT TAAAGTCAAA CAATATGAAA CTTGAAGAAG TAGTTGTGAC TTTGCAGCTT 
GGAGGTGACA AAGAGCCAAC AGAGACAATA GGAGACTTGT CAATTTGTCT TGATGGGCTA 
CAGTTAGAGT CTGAAGTTGT TACCAATGGT GAAACTACAT GTTCAGAAAG TGCTTCTCAG 
AATGATGATG GCTCCAGATC CAAGGATGAA ACAAGAGTGA GCACAAATGG ATCAGATGAC 
CCTGAAGATG CAGGAGCTGG TGAAAATAGG AGAGTCAGTG GGAATAATTC TCCATCACTC 
TCAAATGGTG GTTTTAAACC TTCTAGACCT CCAAGACCTT CACGACCACC ACCACCCACC 
CCACGTAGAC CAGCATCTGT CAATGGTTCA CCATCTGCCA CTTCTGAAAG TGATGGGTCT 
AGTACAGGCT CTCTGCCGCC GACAAATACA AATACAAATA CATCTGAAGG AGCAACATCT 
GGATTAATAA TTCCTCTTAC TATATCTGGA GGCTCAGGCC CTAGGCCATT AAATCCTGTA 
ACTCAAGCTC CCTTGCCACC TGGTTGGGAG CAGAGAGTGG ACCAGCACGG GCGAGTTTAC 
TATGTAGATC ATGTTGAGAA AAGAACAACA TGGGATAGAC CAGAACCTCT ACCTCCTGGC 
TGGGAACGGC GGGTTGACAA CATGGGACGT ATTTATTATG TTGACCATTT CACAAGAACA 
ACAACGTGGC AGAGGCCAAC ACTGGAATCC GTCCGGAACT ATGAACAATG GCAGCTACAG 
CGTAGTCAGC TTCAAGGAGC AATGCAGCAG TTTAACCAGA GATTCATTTA TGGGAATCAA 
GATTTATTTG CTACATCACA AAGTAAAGAA TTTGATCCTC TTGGTCCATT GCCACCTGGA 
TGGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_005260570.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005260627.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu53122
Clone ID Related Accession (Same CDS sequence)	XM_005260570.1
Accession Version	XM_005260570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA AAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA 
AATAGGAGAG TCAGTGGGAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGACCTCCAA GACCTTCACG ACCACCACCA CCCACCCCAC GTAGACCAGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGGTCTAGTA CAGGCTCTCT GCCGCCGACA 
AATACAAATA CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTAACTC AAGCTCCCTT GCCACCTGGT 
TGGGAGCAGA GAGTGGACCA GCACGGGCGA GTTTACTATG TAGATCATGT TGAGAAAAGA 
ACAACATGGG ATAGACCAGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ATTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCAACACTG 
GAATCCGTCC GGAACTATGA ACAATGGCAG CTACAGCGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT CATTTATGGG AATCAAGATT TATTTGCTAC ATCACAAAGT 
AAAGAATTTG ATCCTCTTGG TCCATTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGCAGAGTAT ATTTCGTCAA CCACAACACA CGAATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAAT TAAATGAAAA GCCCTTACCT GAAGGTTGGG AAATGAGATT CACAGTGGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACTACCA CCTATATAGA TCCCCGCACA 
GGAAAATCTG CCCTAGACAA TGGACCTCAG ATAGCCTATG TTCGGGACTT CAAAGCAAAG 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACATTGTT TGAGGATTCC TTTCAACAGA TAATGAGCTT CAGTCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AATGGTTCTT TCTTTTGTCA CATGAAGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATAA ACCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGATTTA TTGCCATGGC TCTGTTCCAT 
GGGAAATTCA TAGACACGGG TTTTTCTTTA CCATTCTATA AGCGTATCTT GAACAAACCA 
GTTGGACTCA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAGGAAAACA ATATTGAGGA ATGTGATTTG GAAATGTACT TCTCCGTTGA CAAAGAAATT 
CTAGGTGAAA TTAAGAGTCA TGATCTGAAA CCTAATGGTG GCAATATTCT TGTAACAGAA 
GAAAATAAAG AGGAATACAT CAGAATGGTA GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA 
GAACAGACAC AAGCTTTCTT TGAAGGCTTT AATGAAATTC TTCCCCAGCA ATATTTGCAA 
TACTTTGATG CAAAGGAATT AGAGGTCCTT TTATGTGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TATGCAAGGA CCAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTAGGAGGA TTTGCTGATC TCATGGGGAG CAATGGACCA 
CAGAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGC TACCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCATACAAG AGCTATGAGC AACTGAAGGA AAAGCTGTTG 
TTTGCCATAG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005260627.1
CDS	640..2718
Translation	MFRKVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_005260570.1
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260570.1

ORF Insert Sequence:

ATGTTCAGAA AAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA 
AATAGGAGAG TCAGTGGGAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGACCTCCAA GACCTTCACG ACCACCACCA CCCACCCCAC GTAGACCAGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGGTCTAGTA CAGGCTCTCT GCCGCCGACA 
AATACAAATA CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTAACTC AAGCTCCCTT GCCACCTGGT 
TGGGAGCAGA GAGTGGACCA GCACGGGCGA GTTTACTATG TAGATCATGT TGAGAAAAGA 
ACAACATGGG ATAGACCAGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ATTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCAACACTG 
GAATCCGTCC GGAACTATGA ACAATGGCAG CTACAGCGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT CATTTATGGG AATCAAGATT TATTTGCTAC ATCACAAAGT 
AAAGAATTTG ATCCTCTTGG TCCATTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGCAGAGTAT ATTTCGTCAA CCACAACACA CGAATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAAT TAAATGAAAA GCCCTTACCT GAAGGTTGGG AAATGAGATT CACAGTGGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACTACCA CCTATATAGA TCCCCGCACA 
GGAAAATCTG CCCTAGACAA TGGACCTCAG ATAGCCTATG TTCGGGACTT CAAAGCAAAG 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACATTGTT TGAGGATTCC TTTCAACAGA TAATGAGCTT CAGTCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AATGGTTCTT TCTTTTGTCA CATGAAGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATAA ACCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGATTTA TTGCCATGGC TCTGTTCCAT 
GGGAAATTCA TAGACACGGG TTTTTCTTTA CCATTCTATA AGCGTATCTT GAACAAACCA 
GTTGGACTCA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAGGAAAACA ATATTGAGGA ATGTGATTTG GAAATGTACT TCTCCGTTGA CAAAGAAATT 
CTAGGTGAAA TTAAGAGTCA TGATCTGAAA CCTAATGGTG GCAATATTCT TGTAACAGAA 
GAAAATAAAG AGGAATACAT CAGAATGGTA GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA 
GAACAGACAC AAGCTTTCTT TGAAGGCTTT AATGAAATTC TTCCCCAGCA ATATTTGCAA 
TACTTTGATG CAAAGGAATT AGAGGTCCTT TTATGTGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TATGCAAGGA CCAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTAGGAGGA TTTGCTGATC TCATGGGGAG CAATGGACCA 
CAGAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGC TACCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCATACAAG AGCTATGAGC AACTGAAGGA AAAGCTGTTG 
TTTGCCATAG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_011529079.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527381.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu73156
Clone ID Related Accession (Same CDS sequence)	XM_011529079.1
Accession Version	XM_011529079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATACA AAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA 
AATAGGAGAG TCAGTGGGAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGACCTCCAA GACCTTCACG ACCACCACCA CCCACCCCAC GTAGACCAGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGGTCTAGTA CAGGCTCTCT GCCGCCGACA 
AATACAAATA CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTAACTC AAGCTCCCTT GCCACCTGGT 
TGGGAGCAGA GAGTGGACCA GCACGGGCGA GTTTACTATG TAGATCATGT TGAGAAAAGA 
ACAACATGGG ATAGACCAGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ATTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCAACACTG 
GAATCCGTCC GGAACTATGA ACAATGGCAG CTACAGCGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT CATTTATGGG AATCAAGATT TATTTGCTAC ATCACAAAGT 
AAAGAATTTG ATCCTCTTGG TCCATTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGCAGAGTAT ATTTCGTCAA CCACAACACA CGAATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAAT TAAATGAAAA GCCCTTACCT GAAGGTTGGG AAATGAGATT CACAGTGGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACTACCA CCTATATAGA TCCCCGCACA 
GGAAAATCTG CCCTAGACAA TGGACCTCAG ATAGCCTATG TTCGGGACTT CAAAGCAAAG 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACATTGTT TGAGGATTCC TTTCAACAGA TAATGAGCTT CAGTCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AATGGTTCTT TCTTTTGTCA CATGAAGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATAA ACCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGATTTA TTGCCATGGC TCTGTTCCAT 
GGGAAATTCA TAGACACGGG TTTTTCTTTA CCATTCTATA AGCGTATCTT GAACAAACCA 
GTTGGACTCA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAGGAAAACA ATATTGAGGA ATGTGATTTG GAAATGTACT TCTCCGTTGA CAAAGAAATT 
CTAGGTGAAA TTAAGAGTCA TGATCTGAAA CCTAATGGTG GCAATATTCT TGTAACAGAA 
GAAAATAAAG AGGAATACAT CAGAATGGTA GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA 
GAACAGACAC AAGCTTTCTT TGAAGGCTTT AATGAAATTC TTCCCCAGCA ATATTTGCAA 
TACTTTGATG CAAAGGAATT AGAGGTCCTT TTATGTGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TATGCAAGGA CCAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTAGGAGGA TTTGCTGATC TCATGGGGAG CAATGGACCA 
CAGAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGC TACCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCATACAAG AGCTATGAGC AACTGAAGGA AAAGCTGTTG 
TTTGCCATAG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527381.1
CDS	660..2738
Translation	MIYKVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_011529079.1
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529079.1

ORF Insert Sequence:

ATGATATACA AAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA 
AATAGGAGAG TCAGTGGGAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGACCTCCAA GACCTTCACG ACCACCACCA CCCACCCCAC GTAGACCAGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGGTCTAGTA CAGGCTCTCT GCCGCCGACA 
AATACAAATA CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTAACTC AAGCTCCCTT GCCACCTGGT 
TGGGAGCAGA GAGTGGACCA GCACGGGCGA GTTTACTATG TAGATCATGT TGAGAAAAGA 
ACAACATGGG ATAGACCAGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ATTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCAACACTG 
GAATCCGTCC GGAACTATGA ACAATGGCAG CTACAGCGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT CATTTATGGG AATCAAGATT TATTTGCTAC ATCACAAAGT 
AAAGAATTTG ATCCTCTTGG TCCATTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGCAGAGTAT ATTTCGTCAA CCACAACACA CGAATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAAT TAAATGAAAA GCCCTTACCT GAAGGTTGGG AAATGAGATT CACAGTGGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACTACCA CCTATATAGA TCCCCGCACA 
GGAAAATCTG CCCTAGACAA TGGACCTCAG ATAGCCTATG TTCGGGACTT CAAAGCAAAG 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACATTGTT TGAGGATTCC TTTCAACAGA TAATGAGCTT CAGTCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AATGGTTCTT TCTTTTGTCA CATGAAGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATAA ACCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGATTTA TTGCCATGGC TCTGTTCCAT 
GGGAAATTCA TAGACACGGG TTTTTCTTTA CCATTCTATA AGCGTATCTT GAACAAACCA 
GTTGGACTCA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAGGAAAACA ATATTGAGGA ATGTGATTTG GAAATGTACT TCTCCGTTGA CAAAGAAATT 
CTAGGTGAAA TTAAGAGTCA TGATCTGAAA CCTAATGGTG GCAATATTCT TGTAACAGAA 
GAAAATAAAG AGGAATACAT CAGAATGGTA GCTGAGTGGA GGTTGTCTCG AGGTGTTGAA 
GAACAGACAC AAGCTTTCTT TGAAGGCTTT AATGAAATTC TTCCCCAGCA ATATTTGCAA 
TACTTTGATG CAAAGGAATT AGAGGTCCTT TTATGTGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TATGCAAGGA CCAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTAGGAGGA TTTGCTGATC TCATGGGGAG CAATGGACCA 
CAGAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGC TACCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCATACAAG AGCTATGAGC AACTGAAGGA AAAGCTGTTG 
TTTGCCATAG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_017028090.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883579.1 Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X3, mRNA.

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CloneID	OHu93130
Clone ID Related Accession (Same CDS sequence)	XM_017028090.1
Accession Version	XM_017028090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG AGACGAGGTT TCACCATCTT GACCAGGCTG GCCTTGAACT CCTGACCTCA 
GGATCCACCC ACCTCGGCCT CCCAAAGTAC AGGGATTACA GGCGTGAGCC ACCGCGTCCG 
GCCTTCATCT CAGCAAAACT TAAGGAAAAT AAGAAGAATT GGTTTGGACC AAGTCCTTAC 
GTAGAGGTCA CAGTAGATGG ACAGTCAAAG AAGACAGAAA AATGCAACAA CACAAACAGT 
CCCAAGTGGA AGCAACCCCT TACAGTTATC GTTACCCCTG TGAGTAAATT ACATTTTCGT 
GTGTGGAGTC ACCAGACACT GAAATCTGAT GTTTTGTTGG GAACTGCTGC ATTAGATATT 
TATGAAACAT TAAAGTCAAA CAATATGAAA CTTGAAGAAG TAGTTGTGAC TTTGCAGCTT 
GGAGGTGACA AAGAGCCAAC AGAGACAATA GGAGACTTGT CAATTTGTCT TGATGGGCTA 
CAGTTAGAGT CTGAAGTTGT TACCAATGGT GAAACTACAT GTTCAGAAAG TGCTTCTCAG 
AATGATGATG GCTCCAGATC CAAGGATGAA ACAAGAGTGA GCACAAATGG ATCAGATGAC 
CCTGAAGATG CAGGAGCTGG TGAAAATAGG AGAGTCAGTG GGAATAATTC TCCATCACTC 
TCAAATGGTG GTTTTAAACC TTCTAGACCT CCAAGACCTT CACGACCACC ACCACCCACC 
CCACGTAGAC CAGCATCTGT CAATGGTTCA CCATCTGCCA CTTCTGAAAG TGATGGGTCT 
AGTACAGGCT CTCTGCCGCC GACAAATACA AATACAAATA CATCTGAAGG AGCAACATCT 
GGATTAATAA TTCCTCTTAC TATATCTGGA GGCTCAGGCC CTAGGCCATT AAATCCTGTA 
ACTCAAGCTC CCTTGCCACC TGGTTGGGAG CAGAGAGTGG ACCAGCACGG GCGAGTTTAC 
TATGTAGATC ATGTTGAGAA AAGAACAACA TGGGATAGAC CAGAACCTCT ACCTCCTGGC 
TGGGAACGGC GGGTTGACAA CATGGGACGT ATTTATTATG TTGACCATTT CACAAGAACA 
ACAACGTGGC AGAGGCCAAC ACTGGAATCC GTCCGGAACT ATGAACAATG GCAGCTACAG 
CGTAGTCAGC TTCAAGGAGC AATGCAGCAG TTTAACCAGA GATTCATTTA TGGGAATCAA 
GATTTATTTG CTACATCACA AAGTAAAGAA TTTGATCCTC TTGGTCCATT GCCACCTGGA 
TGGGAGAAGA GAACAGACAG CAATGGCAGA GTATATTTCG TCAACCACAA CACACGAATT 
ACACAATGGG AAGACCCCAG AAGTCAAGGT CAATTAAATG AAAAGCCCTT ACCTGAAGGT 
TGGGAAATGA GATTCACAGT GGATGGAATT CCATATTTTG TGGACCACAA TAGAAGAACT 
ACCACCTATA TAGATCCCCG CACAGGAAAA TCTGCCCTAG ACAATGGACC TCAGATAGCC 
TATGTTCGGG ACTTCAAAGC AAAGGTTCAG TATTTCCGGT TCTGGTGTCA GCAACTGGCC 
ATGCCACAGC ACATAAAGAT TACAGTGACA AGAAAAACAT TGTTTGAGGA TTCCTTTCAA 
CAGATAATGA GCTTCAGTCC CCAAGATCTG CGAAGACGTT TGTGGGTGAT TTTTCCAGGA 
GAAGAAGGTT TAGATTATGG AGGTGTAGCA AGAGAATGGT TCTTTCTTTT GTCACATGAA 
GTGTTGAACC CAATGTATTG CCTGTTTGAA TATGCAGGGA AGGATAACTA CTGCTTGCAG 
ATAAACCCCG CTTCTTACAT CAATCCAGAT CACCTGAAAT ATTTTCGTTT TATTGGCAGA 
TTTATTGCCA TGGCTCTGTT CCATGGGAAA TTCATAGACA CGGGTTTTTC TTTACCATTC 
TATAAGCGTA TCTTGAACAA ACCAGTTGGA CTCAAGGATT TAGAATCTAT TGATCCAGAA 
TTTTACAATT CTCTCATCTG GGTTAAGGAA AACAATATTG AGGAATGTGA TTTGGAAATG 
TACTTCTCCG TTGACAAAGA AATTCTAGGT GAAATTAAGA GTCATGATCT GAAACCTAAT 
GGTGGCAATA TTCTTGTAAC AGAAGAAAAT AAAGAGGAAT ACATCAGAAT GGTAGCTGAG 
TGGAGGTTGT CTCGAGGTGT TGAAGAACAG ACACAAGCTT TCTTTGAAGG CTTTAATGAA 
ATTCTTCCCC AGCAATATTT GCAATACTTT GATGCAAAGG AATTAGAGGT CCTTTTATGT 
GGAATGCAAG AGATTGATTT GAATGACTGG CAAAGACATG CCATCTACCG TCATTATGCA 
AGGACCAGCA AACAAATCAT GTGGTTTTGG CAGTTTGTTA AAGAAATTGA TAATGAGAAG 
AGAATGAGAC TTCTGCAGTT TGTTACTGGA ACCTGCCGAT TGCCAGTAGG AGGATTTGCT 
GATCTCATGG GGAGCAATGG ACCACAGAAA TTCTGCATTG AAAAAGTTGG GAAAGAAAAT 
TGGCTACCCA GAAGTCATAC CTGTTTTAAT CGCCTGGACC TGCCACCATA CAAGAGCTAT 
GAGCAACTGA AGGAAAAGCT GTTGTTTGCC ATAGAAGAAA CAGAAGGATT TGGACAAGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883579.1
CDS	176..2878
Translation	MSDSGSQLGSMGSLTMKSQLQITETRFHHLDQAGLELLTSGSTHLGLPKYRDYRREPPRPAFISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_017028090.1
Organism	Homo sapiens(human)
Definition	Homo sapiens itchy E3 ubiquitin protein ligase (ITCH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028090.1

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT 
CAGATCACTG AGACGAGGTT TCACCATCTT GACCAGGCTG GCCTTGAACT CCTGACCTCA 
GGATCCACCC ACCTCGGCCT CCCAAAGTAC AGGGATTACA GGCGTGAGCC ACCGCGTCCG 
GCCTTCATCT CAGCAAAACT TAAGGAAAAT AAGAAGAATT GGTTTGGACC AAGTCCTTAC 
GTAGAGGTCA CAGTAGATGG ACAGTCAAAG AAGACAGAAA AATGCAACAA CACAAACAGT 
CCCAAGTGGA AGCAACCCCT TACAGTTATC GTTACCCCTG TGAGTAAATT ACATTTTCGT 
GTGTGGAGTC ACCAGACACT GAAATCTGAT GTTTTGTTGG GAACTGCTGC ATTAGATATT 
TATGAAACAT TAAAGTCAAA CAATATGAAA CTTGAAGAAG TAGTTGTGAC TTTGCAGCTT 
GGAGGTGACA AAGAGCCAAC AGAGACAATA GGAGACTTGT CAATTTGTCT TGATGGGCTA 
CAGTTAGAGT CTGAAGTTGT TACCAATGGT GAAACTACAT GTTCAGAAAG TGCTTCTCAG 
AATGATGATG GCTCCAGATC CAAGGATGAA ACAAGAGTGA GCACAAATGG ATCAGATGAC 
CCTGAAGATG CAGGAGCTGG TGAAAATAGG AGAGTCAGTG GGAATAATTC TCCATCACTC 
TCAAATGGTG GTTTTAAACC TTCTAGACCT CCAAGACCTT CACGACCACC ACCACCCACC 
CCACGTAGAC CAGCATCTGT CAATGGTTCA CCATCTGCCA CTTCTGAAAG TGATGGGTCT 
AGTACAGGCT CTCTGCCGCC GACAAATACA AATACAAATA CATCTGAAGG AGCAACATCT 
GGATTAATAA TTCCTCTTAC TATATCTGGA GGCTCAGGCC CTAGGCCATT AAATCCTGTA 
ACTCAAGCTC CCTTGCCACC TGGTTGGGAG CAGAGAGTGG ACCAGCACGG GCGAGTTTAC 
TATGTAGATC ATGTTGAGAA AAGAACAACA TGGGATAGAC CAGAACCTCT ACCTCCTGGC 
TGGGAACGGC GGGTTGACAA CATGGGACGT ATTTATTATG TTGACCATTT CACAAGAACA 
ACAACGTGGC AGAGGCCAAC ACTGGAATCC GTCCGGAACT ATGAACAATG GCAGCTACAG 
CGTAGTCAGC TTCAAGGAGC AATGCAGCAG TTTAACCAGA GATTCATTTA TGGGAATCAA 
GATTTATTTG CTACATCACA AAGTAAAGAA TTTGATCCTC TTGGTCCATT GCCACCTGGA 
TGGGAGAAGA GAACAGACAG CAATGGCAGA GTATATTTCG TCAACCACAA CACACGAATT 
ACACAATGGG AAGACCCCAG AAGTCAAGGT CAATTAAATG AAAAGCCCTT ACCTGAAGGT 
TGGGAAATGA GATTCACAGT GGATGGAATT CCATATTTTG TGGACCACAA TAGAAGAACT 
ACCACCTATA TAGATCCCCG CACAGGAAAA TCTGCCCTAG ACAATGGACC TCAGATAGCC 
TATGTTCGGG ACTTCAAAGC AAAGGTTCAG TATTTCCGGT TCTGGTGTCA GCAACTGGCC 
ATGCCACAGC ACATAAAGAT TACAGTGACA AGAAAAACAT TGTTTGAGGA TTCCTTTCAA 
CAGATAATGA GCTTCAGTCC CCAAGATCTG CGAAGACGTT TGTGGGTGAT TTTTCCAGGA 
GAAGAAGGTT TAGATTATGG AGGTGTAGCA AGAGAATGGT TCTTTCTTTT GTCACATGAA 
GTGTTGAACC CAATGTATTG CCTGTTTGAA TATGCAGGGA AGGATAACTA CTGCTTGCAG 
ATAAACCCCG CTTCTTACAT CAATCCAGAT CACCTGAAAT ATTTTCGTTT TATTGGCAGA 
TTTATTGCCA TGGCTCTGTT CCATGGGAAA TTCATAGACA CGGGTTTTTC TTTACCATTC 
TATAAGCGTA TCTTGAACAA ACCAGTTGGA CTCAAGGATT TAGAATCTAT TGATCCAGAA 
TTTTACAATT CTCTCATCTG GGTTAAGGAA AACAATATTG AGGAATGTGA TTTGGAAATG 
TACTTCTCCG TTGACAAAGA AATTCTAGGT GAAATTAAGA GTCATGATCT GAAACCTAAT 
GGTGGCAATA TTCTTGTAAC AGAAGAAAAT AAAGAGGAAT ACATCAGAAT GGTAGCTGAG 
TGGAGGTTGT CTCGAGGTGT TGAAGAACAG ACACAAGCTT TCTTTGAAGG CTTTAATGAA 
ATTCTTCCCC AGCAATATTT GCAATACTTT GATGCAAAGG AATTAGAGGT CCTTTTATGT 
GGAATGCAAG AGATTGATTT GAATGACTGG CAAAGACATG CCATCTACCG TCATTATGCA 
AGGACCAGCA AACAAATCAT GTGGTTTTGG CAGTTTGTTA AAGAAATTGA TAATGAGAAG 
AGAATGAGAC TTCTGCAGTT TGTTACTGGA ACCTGCCGAT TGCCAGTAGG AGGATTTGCT 
GATCTCATGG GGAGCAATGG ACCACAGAAA TTCTGCATTG AAAAAGTTGG GAAAGAAAAT 
TGGCTACCCA GAAGTCATAC CTGTTTTAAT CGCCTGGACC TGCCACCATA CAAGAGCTAT 
GAGCAACTGA AGGAAAAGCT GTTGTTTGCC ATAGAAGAAA CAGAAGGATT TGGACAAGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ITCH
Entrez Gene ID	83737
Full Name	itchy E3 ubiquitin protein ligase
Synonyms	ADMFD,AIF4,AIP4,NAPP1
General protein information	Preferred Names itchy E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase Itchy homolog HECT-type E3 ubiquitin transferase Itchy homolog NFE2-associated polypeptide 1 atrophin-1 interacting protein 4 itchy E3 ubiquitin protein ligase homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q11.22
Summary	This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012].
Disorder MIM:	606409
Disorder Html:	Autoimmune disease, multisystem, with facial dysmorphism, 613385 (3)

Homo sapiens (human)	ITCH	NP_113671.3
Canis lupus familiaris (dog)	ITCH	XP_851460.1
Mus musculus (house mouse)	Itch	NP_001230641.1
Rattus norvegicus (Norway rat)	Itch	NP_001005887.1
Bos taurus (cattle)	ITCH	NP_001075897.1
Gallus gallus (chicken)	ITCH	XP_417330.2
Danio rerio (zebrafish)	itch	XP_002667578.2
Danio rerio (zebrafish)	LOC100330031	XP_003201702.2
	LOC707640	XP_002808005.1
Xenopus tropicalis (tropical clawed frog)	itch	NP_001072737.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ITCH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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