TMEM254 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017016680.1	XP_016872169.1	transmembrane protein 254 isoform X4
NM_025125.3	NP_079401.2	transmembrane protein 254 isoform 1
NM_001270369.1	NP_001257298.1	transmembrane protein 254 isoform 3
XM_011540193.3	XP_011538495.1	transmembrane protein 254 isoform X5
NM_001270370.1	NP_001257299.1	transmembrane protein 254 isoform 4
XM_011540192.3	XP_011538494.1	transmembrane protein 254 isoform X3
NM_001270368.1	NP_001257297.1	transmembrane protein 254 isoform 3
NM_001270371.1	NP_001257300.1	transmembrane protein 254 isoform 5
NM_001270373.1	NP_001257302.1	transmembrane protein 254 isoform 6
NM_001270372.1	NP_001257301.1	transmembrane protein 254 isoform 6
XM_006717978.2	XP_006718041.1	transmembrane protein 254 isoform X1
XM_017016679.1	XP_016872168.1	transmembrane protein 254 isoform X2
XM_011540192.2	XP_011538494.1	transmembrane protein 254 isoform X3
XM_011540193.2	XP_011538495.1	transmembrane protein 254 isoform X5
NM_001270374.1	NP_001257303.1	transmembrane protein 254 isoform 7
NM_001270367.1	NP_001257296.1	transmembrane protein 254 isoform 2
XM_017016680.2	XP_016872169.1	transmembrane protein 254 isoform X4
NM_025125.4	NP_079401.2	transmembrane protein 254 isoform 1

The following TMEM254 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM254 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14055	NM_001270367.1 Latest version!	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu14063	NM_025125.4 Latest version!	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu14136	NM_001270372.1 Latest version!	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu09997	NM_001270371.1 Latest version!	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu10052	NM_001270368.1 Latest version!	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu14028	NM_001270370.1 Latest version!	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu14039	NM_001270374.1 Latest version!	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu14113	NM_001270369.1 Latest version!	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu14178	NM_001270373.1 Latest version!	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu10052	XM_011540192.3 Latest version!	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu14039	XM_011540193.3 Latest version!	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu44558	XM_006717978.2 Latest version!	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu91662	XM_017016679.1 Latest version!	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu91663	XM_017016680.2 Latest version!	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu10052	XM_011540192.2	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu14039	XM_011540193.2	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu91663	XM_017016680.1	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu14063	NM_025125.3	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

Reference Sequences (Refseq)

TMEM254 ( NM_001270367.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257296.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 2, mRNA.

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CloneID	OHu14055
Clone ID Related Accession (Same CDS sequence)	NM_001270367.1
Accession Version	NM_001270367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK300490.1, AL832483.1, BC022252.1 and AL832397.1. Transcript Variant: This variant (2) represents the use of an alternate promoter at the 5' end, compared to variant 1. Variant 2 encodes an isoform (2) with a longer and distinct N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AL832483.1, AK300490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGCGG GGACGGCTGG GGAGCTCCCA GCCAACTCTG TGTCTGGGTT TTTCAAGAGG 
ATGGTGAAGT CGGAGGCCAG CAATGCGGCT ACCGCCTATT TCCGTCCAGC AAGACCCTTG 
CCCTCTCTGG TCACAGTCCT GGGGCTGGGC TACTTCGCGT GGGTTGTCTT CTGGCCTCAG 
AGTATCCCTT ATCAGAACCT TGGGCCCCTG GGCCCCTTCA CTCAGTACTT GGTGGACCAC 
CATCACACCC TCCTGTGCAA TGGGTATTGG CTTGCCTGGC TGATTCATGT GGGAGAGTCC 
TTGTATGCCA TAGTATTGTG CAAGCATAAA GGCATCACAA GTGGTCGGGC TCAGCTACTC 
TGGTTCCTAC AGACTTTCTT CTTTGGGATA GCGTCTCTCA CCATCTTGAT TGCTTACAAA 
CGGAAGCGCC AAAAACAAAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257296.1
CDS	4..447
Translation	MGAGTAGELPANSVSGFFKRMVKSEASNAATAYFRPARPLPSLVTVLGLGYFAWVVFWPQSIPYQNLGPLGPFTQYLVDHHHTLLCNGYWLAWLIHVGESLYAIVLCKHKGITSGRAQLLWFLQTFFFGIASLTILIAYKRKRQKQT

Target ORF information:

RefSeq Version	NM_001270367.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270367.1

ORF Insert Sequence:

ATGGGGGCGG GGACGGCTGG GGAGCTCCCA GCCAACTCTG TGTCTGGGTT TTTCAAGAGG 
ATGGTGAAGT CGGAGGCCAG CAATGCGGCT ACCGCCTATT TCCGTCCAGC AAGACCCTTG 
CCCTCTCTGG TCACAGTCCT GGGGCTGGGC TACTTCGCGT GGGTTGTCTT CTGGCCTCAG 
AGTATCCCTT ATCAGAACCT TGGGCCCCTG GGCCCCTTCA CTCAGTACTT GGTGGACCAC 
CATCACACCC TCCTGTGCAA TGGGTATTGG CTTGCCTGGC TGATTCATGT GGGAGAGTCC 
TTGTATGCCA TAGTATTGTG CAAGCATAAA GGCATCACAA GTGGTCGGGC TCAGCTACTC 
TGGTTCCTAC AGACTTTCTT CTTTGGGATA GCGTCTCTCA CCATCTTGAT TGCTTACAAA 
CGGAAGCGCC AAAAACAAAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( NM_025125.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_079401.2 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 1, mRNA.

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CloneID	OHu14063
Clone ID Related Accession (Same CDS sequence)	NM_025125.3 , NM_025125.4
Accession Version	NM_025125.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA520338.1, BC022252.1 and AL832397.1. On Jun 28, 2012 this sequence version replaced NM_025125.2. Transcript Variant: This variant (1) represents the most common transcript and encodes isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC022252.1, SRR1660803.211854.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTACGG CAGCCGGCGC GACCTACTTT CAGCGAGGCA GTCTGTTCTG GTTCACAGTC 
ATCACCCTCA GCTTTGGCTA CTACACATGG GTTGTCTTCT GGCCTCAGAG TATCCCTTAT 
CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG TGGACCACCA TCACACCCTC 
CTGTGCAATG GGTATTGGCT TGCCTGGCTG ATTCATGTGG GAGAGTCCTT GTATGCCATA 
GTATTGTGCA AGCATAAAGG CATCACAAGT GGTCGGGCTC AGCTACTCTG GTTCCTACAG 
ACTTTCTTCT TTGGGATAGC GTCTCTCACC ATCTTGATTG CTTACAAACG GAAGCGCCAA 
AAACAAACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079401.2
CDS	55..426
Translation	MATAAGATYFQRGSLFWFTVITLSFGYYTWVVFWPQSIPYQNLGPLGPFTQYLVDHHHTLLCNGYWLAWLIHVGESLYAIVLCKHKGITSGRAQLLWFLQTFFFGIASLTILIAYKRKRQKQT

Target ORF information:

RefSeq Version	NM_025125.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025125.3

ORF Insert Sequence:

ATGGCTACGG CAGCCGGCGC GACCTACTTT CAGCGAGGCA GTCTGTTCTG GTTCACAGTC 
ATCACCCTCA GCTTTGGCTA CTACACATGG GTTGTCTTCT GGCCTCAGAG TATCCCTTAT 
CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG TGGACCACCA TCACACCCTC 
CTGTGCAATG GGTATTGGCT TGCCTGGCTG ATTCATGTGG GAGAGTCCTT GTATGCCATA 
GTATTGTGCA AGCATAAAGG CATCACAAGT GGTCGGGCTC AGCTACTCTG GTTCCTACAG 
ACTTTCTTCT TTGGGATAGC GTCTCTCACC ATCTTGATTG CTTACAAACG GAAGCGCCAA 
AAACAAACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( NM_025125.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_079401.2 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14063
Clone ID Related Accession (Same CDS sequence)	NM_025125.3 , NM_025125.4
Accession Version	NM_025125.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA520338.1, BC022252.1 and AL832397.1. On Nov 22, 2018 this sequence version replaced NM_025125.3. Transcript Variant: This variant (1) represents the most common transcript and encodes isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.244658.1, SRR1660803.211854.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000372281.8/ ENSP00000361355.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTACGG CAGCCGGCGC GACCTACTTT CAGCGAGGCA GTCTGTTCTG GTTCACAGTC 
ATCACCCTCA GCTTTGGCTA CTACACATGG GTTGTCTTCT GGCCTCAGAG TATCCCTTAT 
CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG TGGACCACCA TCACACCCTC 
CTGTGCAATG GGTATTGGCT TGCCTGGCTG ATTCATGTGG GAGAGTCCTT GTATGCCATA 
GTATTGTGCA AGCATAAAGG CATCACAAGT GGTCGGGCTC AGCTACTCTG GTTCCTACAG 
ACTTTCTTCT TTGGGATAGC GTCTCTCACC ATCTTGATTG CTTACAAACG GAAGCGCCAA 
AAACAAACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079401.2
CDS	36..407
Translation	MATAAGATYFQRGSLFWFTVITLSFGYYTWVVFWPQSIPYQNLGPLGPFTQYLVDHHHTLLCNGYWLAWLIHVGESLYAIVLCKHKGITSGRAQLLWFLQTFFFGIASLTILIAYKRKRQKQT

Target ORF information:

RefSeq Version	NM_025125.4
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025125.4

ORF Insert Sequence:

ATGGCTACGG CAGCCGGCGC GACCTACTTT CAGCGAGGCA GTCTGTTCTG GTTCACAGTC 
ATCACCCTCA GCTTTGGCTA CTACACATGG GTTGTCTTCT GGCCTCAGAG TATCCCTTAT 
CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG TGGACCACCA TCACACCCTC 
CTGTGCAATG GGTATTGGCT TGCCTGGCTG ATTCATGTGG GAGAGTCCTT GTATGCCATA 
GTATTGTGCA AGCATAAAGG CATCACAAGT GGTCGGGCTC AGCTACTCTG GTTCCTACAG 
ACTTTCTTCT TTGGGATAGC GTCTCTCACC ATCTTGATTG CTTACAAACG GAAGCGCCAA 
AAACAAACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( NM_001270372.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257301.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu14136
Clone ID Related Accession (Same CDS sequence)	NM_001270372.1
Accession Version	NM_001270372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA520338.1, DA930892.1, AL832397.1 and BC022252.1. Transcript Variant: This variant (7) contains an alternate segment in the 3' coding region which introduces an early stop codon, compared to variant 1. Variant 7 encodes an isoform (6) with a shorter C-terminus, compared to isoform 1. Variants 7 and 8 encode the same isoform (6) but their stop codons reside on different exons. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.88638.1, SRR1660805.225828.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTACGG CAGCCGGCGC GACCTACTTT CAGCGAGGCA GTCTGTTCTG GTTCACAGTC 
ATCACCCTCA GCTTTGGCTA CTACACATGG GTTGTCTTCT GGCCTCAGAG TATCCCTTAT 
CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG TGGACCACCA TCACACCCTC 
CTGTGCAATG GGTATTGGCT TGCCTGGCTG ATTCATGTGG GAGAGTCCTT GTATGCCATA 
GTATTGTGCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257301.1
CDS	55..309
Translation	MATAAGATYFQRGSLFWFTVITLSFGYYTWVVFWPQSIPYQNLGPLGPFTQYLVDHHHTLLCNGYWLAWLIHVGESLYAIVLCK

Target ORF information:

RefSeq Version	NM_001270372.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270372.1

ORF Insert Sequence:

ATGGCTACGG CAGCCGGCGC GACCTACTTT CAGCGAGGCA GTCTGTTCTG GTTCACAGTC 
ATCACCCTCA GCTTTGGCTA CTACACATGG GTTGTCTTCT GGCCTCAGAG TATCCCTTAT 
CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG TGGACCACCA TCACACCCTC 
CTGTGCAATG GGTATTGGCT TGCCTGGCTG ATTCATGTGG GAGAGTCCTT GTATGCCATA 
GTATTGTGCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( NM_001270371.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257300.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu09997
Clone ID Related Accession (Same CDS sequence)	NM_001270371.1
Accession Version	NM_001270371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK309425.1, AL832397.1 and BC022252.1. Transcript Variant: This variant (6) contains an alternate exon in the 5' coding region, compared to variant 1, which introduces a frameshift and results in an isoform (5) with a shorter and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.193220.1, AK309425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146982, SAMN03267759 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTACGG CAGCCGGCGC GACCTACTTT CAGCGAGGCA GTCTGTTCTG GTTCACAGTC 
ATCACCCTCA GCTTTGGCTA CTACACACCC CAGCAGCGGA GTGAGACCCT GTCAAAAAAA 
AAAGAAAGAA AGAAAATAGA AGCCAAGAAT GGAGACCCAA ATGACTGTTC AGAGTTTCTT 
CGTGGGTTGT CTTCTGGCCT CAGAGTATCC CTTATCAGAA CCTTGGGCCC CTGGGCCCCT 
TCACTCAGTA CTTGGTGGAC CACCATCACA CCCTCCTGTG CAATGGGTAT TGGCTTGCCT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257300.1
CDS	55..360
Translation	MATAAGATYFQRGSLFWFTVITLSFGYYTPQQRSETLSKKKERKKIEAKNGDPNDCSEFLRGLSSGLRVSLIRTLGPWAPSLSTWWTTITPSCAMGIGLPG

Target ORF information:

RefSeq Version	NM_001270371.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270371.1

ORF Insert Sequence:

ATGGCTACGG CAGCCGGCGC GACCTACTTT CAGCGAGGCA GTCTGTTCTG GTTCACAGTC 
ATCACCCTCA GCTTTGGCTA CTACACACCC CAGCAGCGGA GTGAGACCCT GTCAAAAAAA 
AAAGAAAGAA AGAAAATAGA AGCCAAGAAT GGAGACCCAA ATGACTGTTC AGAGTTTCTT 
CGTGGGTTGT CTTCTGGCCT CAGAGTATCC CTTATCAGAA CCTTGGGCCC CTGGGCCCCT 
TCACTCAGTA CTTGGTGGAC CACCATCACA CCCTCCTGTG CAATGGGTAT TGGCTTGCCT 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( NM_001270368.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257297.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu10052
Clone ID Related Accession (Same CDS sequence)	XM_011540192.3 , NM_001270368.1 , XM_011540192.2
Accession Version	NM_001270368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK300490.1, AK309980.1, AL832397.1 and BC022252.1. Transcript Variant: This variant (3) represents the use of an alternate promoter at the 5' end and contains an alternate segment in the 3' coding region which introduces an early stop codon, compared to variant 1. Variant 3 encodes an isoform (3) with a longer and distinct N-terminus but shorter C-terminus, compared to isoform 1. Variants 3 and 4 encode the same isoform (3) but their stop codons reside on different exons. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.76722.1, AK309980.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGCGG GGACGGCTGG GGAGCTCCCA GCCAACTCTG TGTCTGGGTT TTTCAAGAGG 
ATGGTGAAGT CGGAGGCCAG CAATGCGGCT ACCGCCTATT TCCGTCCAGC AAGACCCTTG 
CCCTCTCTGG TCACAGTCCT GGGGCTGGGC TACTTCGCGT GGGTTGTCTT CTGGCCTCAG 
AGTATCCCTT ATCAGAACCT TGGGCCCCTG GGCCCCTTCA CTCAGTACTT GGTGGACCAC 
CATCACACCC TCCTGTGCAA TGGGTATTGG CTTGCCTGGC TGATTCATGT GGGAGAGTCC 
TTGTATGCCA TAGTATTGTG CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257297.1
CDS	4..330
Translation	MGAGTAGELPANSVSGFFKRMVKSEASNAATAYFRPARPLPSLVTVLGLGYFAWVVFWPQSIPYQNLGPLGPFTQYLVDHHHTLLCNGYWLAWLIHVGESLYAIVLCK

Target ORF information:

RefSeq Version	NM_001270368.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270368.1

ORF Insert Sequence:

ATGGGGGCGG GGACGGCTGG GGAGCTCCCA GCCAACTCTG TGTCTGGGTT TTTCAAGAGG 
ATGGTGAAGT CGGAGGCCAG CAATGCGGCT ACCGCCTATT TCCGTCCAGC AAGACCCTTG 
CCCTCTCTGG TCACAGTCCT GGGGCTGGGC TACTTCGCGT GGGTTGTCTT CTGGCCTCAG 
AGTATCCCTT ATCAGAACCT TGGGCCCCTG GGCCCCTTCA CTCAGTACTT GGTGGACCAC 
CATCACACCC TCCTGTGCAA TGGGTATTGG CTTGCCTGGC TGATTCATGT GGGAGAGTCC 
TTGTATGCCA TAGTATTGTG CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( NM_001270370.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257299.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu14028
Clone ID Related Accession (Same CDS sequence)	NM_001270370.1
Accession Version	NM_001270370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA520338.1, CX756014.1, AL832397.1 and BC022252.1. Transcript Variant: This variant (5) contains two alternate exons in the 3' coding region, compared to variant 1, which result in an isoform (4) with a shorter and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: CX756014.1, AW881125.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTACGG CAGCCGGCGC GACCTACTTT CAGCGAGGCA GTCTGTTCTG GTTCACAGTC 
ATCACCCTCA GCTTTGGCTA CTACACATGG GTTGTCTTCT GGCCTCAGAG TATCCCTTAT 
CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG TGGACCACCA TCACACCCTC 
CTGTGCAATG GGTATTGGCT TGCCTGGCTG ATTCATGTGG GAGAGTCCTT GTATGCCATA 
GTATTGTGCA AGCAAAATGA GCTAAGAATA CGGATAAAGA TGAGAAAACT GATTCTGAGA 
GATTCTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257299.1
CDS	55..366
Translation	MATAAGATYFQRGSLFWFTVITLSFGYYTWVVFWPQSIPYQNLGPLGPFTQYLVDHHHTLLCNGYWLAWLIHVGESLYAIVLCKQNELRIRIKMRKLILRDSE

Target ORF information:

RefSeq Version	NM_001270370.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270370.1

ORF Insert Sequence:

ATGGCTACGG CAGCCGGCGC GACCTACTTT CAGCGAGGCA GTCTGTTCTG GTTCACAGTC 
ATCACCCTCA GCTTTGGCTA CTACACATGG GTTGTCTTCT GGCCTCAGAG TATCCCTTAT 
CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG TGGACCACCA TCACACCCTC 
CTGTGCAATG GGTATTGGCT TGCCTGGCTG ATTCATGTGG GAGAGTCCTT GTATGCCATA 
GTATTGTGCA AGCAAAATGA GCTAAGAATA CGGATAAAGA TGAGAAAACT GATTCTGAGA 
GATTCTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( NM_001270374.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257303.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu14039
Clone ID Related Accession (Same CDS sequence)	XM_011540193.3 , XM_011540193.2 , NM_001270374.1
Accession Version	NM_001270374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform 7
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP204746.1, AK310160.1, DA880596.1, AL832397.1 and BC022252.1. Transcript Variant: This variant (9) represents the use of an alternate promoter at the 5' end and contains an alternate exon in the 5' coding region, compared to variant 1. Variant 9 encodes an isoform (7) that lacks any sequence similarity to isoform 1 but whose C-terminus is identical to isoform 5. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.167666.1, BI459537.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTGGAA AACCCCAGCA GCGGAGTGAG ACCCTGTCAA AAAAAAAAGA AAGAAAGAAA 
ATAGAAGCCA AGAATGGAGA CCCAAATGAC TGTTCAGAGT TTCTTCGTGG GTTGTCTTCT 
GGCCTCAGAG TATCCCTTAT CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG 
TGGACCACCA TCACACCCTC CTGTGCAATG GGTATTGGCT TGCCTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257303.1
CDS	437..667
Translation	MRGKPQQRSETLSKKKERKKIEAKNGDPNDCSEFLRGLSSGLRVSLIRTLGPWAPSLSTWWTTITPSCAMGIGLPG

Target ORF information:

RefSeq Version	NM_001270374.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270374.1

ORF Insert Sequence:

ATGCGTGGAA AACCCCAGCA GCGGAGTGAG ACCCTGTCAA AAAAAAAAGA AAGAAAGAAA 
ATAGAAGCCA AGAATGGAGA CCCAAATGAC TGTTCAGAGT TTCTTCGTGG GTTGTCTTCT 
GGCCTCAGAG TATCCCTTAT CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG 
TGGACCACCA TCACACCCTC CTGTGCAATG GGTATTGGCT TGCCTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( NM_001270369.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257298.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu14113
Clone ID Related Accession (Same CDS sequence)	NM_001270369.1
Accession Version	NM_001270369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK300490.1, BP293793.1, AK309980.1, AL832397.1 and BC022252.1. Transcript Variant: This variant (4) represents the use of an alternate promoter at the 5' end and contains an alternate exon in the 3' coding region which introduces an early stop codon, compared to variant 1. Variant 4 encodes an isoform (3) with a longer and distinct N-terminus but shorter C-terminus, compared to isoform 1. Variants 3 and 4 encode the same isoform (3) but their stop codons reside on different exons. ##Evidence-Data-START## Transcript exon combination :: BX377457.2, BP293793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGCGG GGACGGCTGG GGAGCTCCCA GCCAACTCTG TGTCTGGGTT TTTCAAGAGG 
ATGGTGAAGT CGGAGGCCAG CAATGCGGCT ACCGCCTATT TCCGTCCAGC AAGACCCTTG 
CCCTCTCTGG TCACAGTCCT GGGGCTGGGC TACTTCGCGT GGGTTGTCTT CTGGCCTCAG 
AGTATCCCTT ATCAGAACCT TGGGCCCCTG GGCCCCTTCA CTCAGTACTT GGTGGACCAC 
CATCACACCC TCCTGTGCAA TGGGTATTGG CTTGCCTGGC TGATTCATGT GGGAGAGTCC 
TTGTATGCCA TAGTATTGTG CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257298.1
CDS	4..330
Translation	MGAGTAGELPANSVSGFFKRMVKSEASNAATAYFRPARPLPSLVTVLGLGYFAWVVFWPQSIPYQNLGPLGPFTQYLVDHHHTLLCNGYWLAWLIHVGESLYAIVLCK

Target ORF information:

RefSeq Version	NM_001270369.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270369.1

ORF Insert Sequence:

ATGGGGGCGG GGACGGCTGG GGAGCTCCCA GCCAACTCTG TGTCTGGGTT TTTCAAGAGG 
ATGGTGAAGT CGGAGGCCAG CAATGCGGCT ACCGCCTATT TCCGTCCAGC AAGACCCTTG 
CCCTCTCTGG TCACAGTCCT GGGGCTGGGC TACTTCGCGT GGGTTGTCTT CTGGCCTCAG 
AGTATCCCTT ATCAGAACCT TGGGCCCCTG GGCCCCTTCA CTCAGTACTT GGTGGACCAC 
CATCACACCC TCCTGTGCAA TGGGTATTGG CTTGCCTGGC TGATTCATGT GGGAGAGTCC 
TTGTATGCCA TAGTATTGTG CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( NM_001270373.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257302.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu14178
Clone ID Related Accession (Same CDS sequence)	NM_001270373.1
Accession Version	NM_001270373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA520338.1, DA573956.1, AL832397.1 and BC022252.1. Transcript Variant: This variant (8) contains an alternate exon in the 3' coding region which introduces an early stop codon, compared to variant 1. Variant 8 encodes an isoform (6) with a shorter C-terminus, compared to isoform 1. Variants 7 and 8 encode the same isoform (6) but their stop codons reside on different exons. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.110705.1, SRR1660807.220681.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTACGG CAGCCGGCGC GACCTACTTT CAGCGAGGCA GTCTGTTCTG GTTCACAGTC 
ATCACCCTCA GCTTTGGCTA CTACACATGG GTTGTCTTCT GGCCTCAGAG TATCCCTTAT 
CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG TGGACCACCA TCACACCCTC 
CTGTGCAATG GGTATTGGCT TGCCTGGCTG ATTCATGTGG GAGAGTCCTT GTATGCCATA 
GTATTGTGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257302.1
CDS	55..309
Translation	MATAAGATYFQRGSLFWFTVITLSFGYYTWVVFWPQSIPYQNLGPLGPFTQYLVDHHHTLLCNGYWLAWLIHVGESLYAIVLCK

Target ORF information:

RefSeq Version	NM_001270373.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270373.1

ORF Insert Sequence:

ATGGCTACGG CAGCCGGCGC GACCTACTTT CAGCGAGGCA GTCTGTTCTG GTTCACAGTC 
ATCACCCTCA GCTTTGGCTA CTACACATGG GTTGTCTTCT GGCCTCAGAG TATCCCTTAT 
CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG TGGACCACCA TCACACCCTC 
CTGTGCAATG GGTATTGGCT TGCCTGGCTG ATTCATGTGG GAGAGTCCTT GTATGCCATA 
GTATTGTGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( XM_011540192.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538494.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu10052
Clone ID Related Accession (Same CDS sequence)	XM_011540192.3 , NM_001270368.1 , XM_011540192.2
Accession Version	XM_011540192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011540192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCGG GGACGGCTGG GGAGCTCCCA GCCAACTCTG TGTCTGGGTT TTTCAAGAGG 
ATGGTGAAGT CGGAGGCCAG CAATGCGGCT ACCGCCTATT TCCGTCCAGC AAGACCCTTG 
CCCTCTCTGG TCACAGTCCT GGGGCTGGGC TACTTCGCGT GGGTTGTCTT CTGGCCTCAG 
AGTATCCCTT ATCAGAACCT TGGGCCCCTG GGCCCCTTCA CTCAGTACTT GGTGGACCAC 
CATCACACCC TCCTGTGCAA TGGGTATTGG CTTGCCTGGC TGATTCATGT GGGAGAGTCC 
TTGTATGCCA TAGTATTGTG CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538494.1
CDS	95..421
Translation	MGAGTAGELPANSVSGFFKRMVKSEASNAATAYFRPARPLPSLVTVLGLGYFAWVVFWPQSIPYQNLGPLGPFTQYLVDHHHTLLCNGYWLAWLIHVGESLYAIVLCK

Target ORF information:

RefSeq Version	XM_011540192.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540192.3

ORF Insert Sequence:

ATGGGGGCGG GGACGGCTGG GGAGCTCCCA GCCAACTCTG TGTCTGGGTT TTTCAAGAGG 
ATGGTGAAGT CGGAGGCCAG CAATGCGGCT ACCGCCTATT TCCGTCCAGC AAGACCCTTG 
CCCTCTCTGG TCACAGTCCT GGGGCTGGGC TACTTCGCGT GGGTTGTCTT CTGGCCTCAG 
AGTATCCCTT ATCAGAACCT TGGGCCCCTG GGCCCCTTCA CTCAGTACTT GGTGGACCAC 
CATCACACCC TCCTGTGCAA TGGGTATTGG CTTGCCTGGC TGATTCATGT GGGAGAGTCC 
TTGTATGCCA TAGTATTGTG CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( XM_011540192.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538494.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu10052
Clone ID Related Accession (Same CDS sequence)	XM_011540192.3 , NM_001270368.1 , XM_011540192.2
Accession Version	XM_011540192.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540192.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCGG GGACGGCTGG GGAGCTCCCA GCCAACTCTG TGTCTGGGTT TTTCAAGAGG 
ATGGTGAAGT CGGAGGCCAG CAATGCGGCT ACCGCCTATT TCCGTCCAGC AAGACCCTTG 
CCCTCTCTGG TCACAGTCCT GGGGCTGGGC TACTTCGCGT GGGTTGTCTT CTGGCCTCAG 
AGTATCCCTT ATCAGAACCT TGGGCCCCTG GGCCCCTTCA CTCAGTACTT GGTGGACCAC 
CATCACACCC TCCTGTGCAA TGGGTATTGG CTTGCCTGGC TGATTCATGT GGGAGAGTCC 
TTGTATGCCA TAGTATTGTG CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538494.1
CDS	95..421
Translation	MGAGTAGELPANSVSGFFKRMVKSEASNAATAYFRPARPLPSLVTVLGLGYFAWVVFWPQSIPYQNLGPLGPFTQYLVDHHHTLLCNGYWLAWLIHVGESLYAIVLCK

Target ORF information:

RefSeq Version	XM_011540192.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540192.2

ORF Insert Sequence:

ATGGGGGCGG GGACGGCTGG GGAGCTCCCA GCCAACTCTG TGTCTGGGTT TTTCAAGAGG 
ATGGTGAAGT CGGAGGCCAG CAATGCGGCT ACCGCCTATT TCCGTCCAGC AAGACCCTTG 
CCCTCTCTGG TCACAGTCCT GGGGCTGGGC TACTTCGCGT GGGTTGTCTT CTGGCCTCAG 
AGTATCCCTT ATCAGAACCT TGGGCCCCTG GGCCCCTTCA CTCAGTACTT GGTGGACCAC 
CATCACACCC TCCTGTGCAA TGGGTATTGG CTTGCCTGGC TGATTCATGT GGGAGAGTCC 
TTGTATGCCA TAGTATTGTG CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( XM_011540193.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538495.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu14039
Clone ID Related Accession (Same CDS sequence)	XM_011540193.3 , XM_011540193.2 , NM_001270374.1
Accession Version	XM_011540193.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011540193.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAA AACCCCAGCA GCGGAGTGAG ACCCTGTCAA AAAAAAAAGA AAGAAAGAAA 
ATAGAAGCCA AGAATGGAGA CCCAAATGAC TGTTCAGAGT TTCTTCGTGG GTTGTCTTCT 
GGCCTCAGAG TATCCCTTAT CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG 
TGGACCACCA TCACACCCTC CTGTGCAATG GGTATTGGCT TGCCTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538495.1
CDS	368..598
Translation	MRGKPQQRSETLSKKKERKKIEAKNGDPNDCSEFLRGLSSGLRVSLIRTLGPWAPSLSTWWTTITPSCAMGIGLPG

Target ORF information:

RefSeq Version	XM_011540193.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540193.3

ORF Insert Sequence:

ATGCGTGGAA AACCCCAGCA GCGGAGTGAG ACCCTGTCAA AAAAAAAAGA AAGAAAGAAA 
ATAGAAGCCA AGAATGGAGA CCCAAATGAC TGTTCAGAGT TTCTTCGTGG GTTGTCTTCT 
GGCCTCAGAG TATCCCTTAT CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG 
TGGACCACCA TCACACCCTC CTGTGCAATG GGTATTGGCT TGCCTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( XM_011540193.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538495.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu14039
Clone ID Related Accession (Same CDS sequence)	XM_011540193.3 , XM_011540193.2 , NM_001270374.1
Accession Version	XM_011540193.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAA AACCCCAGCA GCGGAGTGAG ACCCTGTCAA AAAAAAAAGA AAGAAAGAAA 
ATAGAAGCCA AGAATGGAGA CCCAAATGAC TGTTCAGAGT TTCTTCGTGG GTTGTCTTCT 
GGCCTCAGAG TATCCCTTAT CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG 
TGGACCACCA TCACACCCTC CTGTGCAATG GGTATTGGCT TGCCTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538495.1
CDS	368..598
Translation	MRGKPQQRSETLSKKKERKKIEAKNGDPNDCSEFLRGLSSGLRVSLIRTLGPWAPSLSTWWTTITPSCAMGIGLPG

Target ORF information:

RefSeq Version	XM_011540193.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540193.2

ORF Insert Sequence:

ATGCGTGGAA AACCCCAGCA GCGGAGTGAG ACCCTGTCAA AAAAAAAAGA AAGAAAGAAA 
ATAGAAGCCA AGAATGGAGA CCCAAATGAC TGTTCAGAGT TTCTTCGTGG GTTGTCTTCT 
GGCCTCAGAG TATCCCTTAT CAGAACCTTG GGCCCCTGGG CCCCTTCACT CAGTACTTGG 
TGGACCACCA TCACACCCTC CTGTGCAATG GGTATTGGCT TGCCTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( XM_006717978.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718041.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu44558
Clone ID Related Accession (Same CDS sequence)	XM_006717978.2
Accession Version	XM_006717978.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717978.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAA AACCCCAGCA GCGGAGTGAG ACCCTGTCAA AAAAAAAAGA AAGAAAGAAA 
ATAGAAGCCA AGAATGGAGA CCCAAATGAC TGTTCAGAGT TTCTTCGGTC TGTGTGGGTT 
GTCTTCTGGC CTCAGAGTAT CCCTTATCAG AACCTTGGGC CCCTGGGCCC CTTCACTCAG 
TACTTGGTGG ACCACCATCA CACCCTCCTG TGCAATGGGT ATTGGCTTGC CTGGCTGATT 
CATGTGGGAG AGTCCTTGTA TGCCATAGTA TTGTGCAAGC ATAAAGGCAT CACAAGTGGT 
CGGGCTCAGC TACTCTGGTT CCTACAGACT TTCTTCTTTG GGATAGCGTC TCTCACCATC 
TTGATTGCTT ACAAACGGAA GCGCCAAAAA CAAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718041.1
CDS	380..778
Translation	MRGKPQQRSETLSKKKERKKIEAKNGDPNDCSEFLRSVWVVFWPQSIPYQNLGPLGPFTQYLVDHHHTLLCNGYWLAWLIHVGESLYAIVLCKHKGITSGRAQLLWFLQTFFFGIASLTILIAYKRKRQKQT

Target ORF information:

RefSeq Version	XM_006717978.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717978.2

ORF Insert Sequence:

ATGCGTGGAA AACCCCAGCA GCGGAGTGAG ACCCTGTCAA AAAAAAAAGA AAGAAAGAAA 
ATAGAAGCCA AGAATGGAGA CCCAAATGAC TGTTCAGAGT TTCTTCGGTC TGTGTGGGTT 
GTCTTCTGGC CTCAGAGTAT CCCTTATCAG AACCTTGGGC CCCTGGGCCC CTTCACTCAG 
TACTTGGTGG ACCACCATCA CACCCTCCTG TGCAATGGGT ATTGGCTTGC CTGGCTGATT 
CATGTGGGAG AGTCCTTGTA TGCCATAGTA TTGTGCAAGC ATAAAGGCAT CACAAGTGGT 
CGGGCTCAGC TACTCTGGTT CCTACAGACT TTCTTCTTTG GGATAGCGTC TCTCACCATC 
TTGATTGCTT ACAAACGGAA GCGCCAAAAA CAAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( XM_017016679.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872168.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu91662
Clone ID Related Accession (Same CDS sequence)	XM_017016679.1
Accession Version	XM_017016679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCGG GGACGGCTGG GGAGCTCCCA GCCAACTCTG TGTCTGGGTT TTTCAAGAGG 
ATGGTGAAGT CGGAGGCCAG CAATGCGGCT ACCGCCTATT TCCGTCCAGC AAGACCCTTG 
CCCTCTCTGG TCACAGTCCT GGGGCTGGGC TACTTCGCGT GGGTTGTCTT CTGGCCTCAG 
AGTATCCCTT ATCAGAACCT TGGGCCCCTG GGCCCCTTCA CTCAGTACTT GGTGGACCAC 
CATCACACCC TCCTGTGCAA TGGGTATTGG CTTGCCTGGC TGATTCATGT GGGAGAGTCC 
TTGTATGCCA TAGTATTGTG CAAGCAAAAT GAGCTAAGAA TACGGATAAA GATGAGAAAA 
CTGATTCTGA GAGATTCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872168.1
CDS	95..478
Translation	MGAGTAGELPANSVSGFFKRMVKSEASNAATAYFRPARPLPSLVTVLGLGYFAWVVFWPQSIPYQNLGPLGPFTQYLVDHHHTLLCNGYWLAWLIHVGESLYAIVLCKQNELRIRIKMRKLILRDSE

Target ORF information:

RefSeq Version	XM_017016679.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016679.1

ORF Insert Sequence:

ATGGGGGCGG GGACGGCTGG GGAGCTCCCA GCCAACTCTG TGTCTGGGTT TTTCAAGAGG 
ATGGTGAAGT CGGAGGCCAG CAATGCGGCT ACCGCCTATT TCCGTCCAGC AAGACCCTTG 
CCCTCTCTGG TCACAGTCCT GGGGCTGGGC TACTTCGCGT GGGTTGTCTT CTGGCCTCAG 
AGTATCCCTT ATCAGAACCT TGGGCCCCTG GGCCCCTTCA CTCAGTACTT GGTGGACCAC 
CATCACACCC TCCTGTGCAA TGGGTATTGG CTTGCCTGGC TGATTCATGT GGGAGAGTCC 
TTGTATGCCA TAGTATTGTG CAAGCAAAAT GAGCTAAGAA TACGGATAAA GATGAGAAAA 
CTGATTCTGA GAGATTCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( XM_017016680.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872169.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu91663
Clone ID Related Accession (Same CDS sequence)	XM_017016680.1 , XM_017016680.2
Accession Version	XM_017016680.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAA AACCCCAGCA GCGGAGTGAG ACCCTGTCAA AAAAAAAAGA AAGAAAGAAA 
ATAGAAGCCA AGAATGGAGA CCCAAATGAC TGTTCAGAGT TTCTTCGGTC TGTGTGGGTT 
GTCTTCTGGC CTCAGAGTAT CCCTTATCAG AACCTTGGGC CCCTGGGCCC CTTCACTCAG 
TACTTGGTGG ACCACCATCA CACCCTCCTG TGCAATGGGT ATTGGCTTGC CTGGCTGATT 
CATGTGGGAG AGTCCTTGTA TGCCATAGTA TTGTGCAAGC AAAATGAGCT AAGAATACGG 
ATAAAGGTAT GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872169.1
CDS	380..697
Translation	MRGKPQQRSETLSKKKERKKIEAKNGDPNDCSEFLRSVWVVFWPQSIPYQNLGPLGPFTQYLVDHHHTLLCNGYWLAWLIHVGESLYAIVLCKQNELRIRIKVCE

Target ORF information:

RefSeq Version	XM_017016680.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016680.1

ORF Insert Sequence:

ATGCGTGGAA AACCCCAGCA GCGGAGTGAG ACCCTGTCAA AAAAAAAAGA AAGAAAGAAA 
ATAGAAGCCA AGAATGGAGA CCCAAATGAC TGTTCAGAGT TTCTTCGGTC TGTGTGGGTT 
GTCTTCTGGC CTCAGAGTAT CCCTTATCAG AACCTTGGGC CCCTGGGCCC CTTCACTCAG 
TACTTGGTGG ACCACCATCA CACCCTCCTG TGCAATGGGT ATTGGCTTGC CTGGCTGATT 
CATGTGGGAG AGTCCTTGTA TGCCATAGTA TTGTGCAAGC AAAATGAGCT AAGAATACGG 
ATAAAGGTAT GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM254 ( XM_017016680.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872169.1 Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu91663
Clone ID Related Accession (Same CDS sequence)	XM_017016680.1 , XM_017016680.2
Accession Version	XM_017016680.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transmembrane protein 254 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016680.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGGAA AACCCCAGCA GCGGAGTGAG ACCCTGTCAA AAAAAAAAGA AAGAAAGAAA 
ATAGAAGCCA AGAATGGAGA CCCAAATGAC TGTTCAGAGT TTCTTCGGTC TGTGTGGGTT 
GTCTTCTGGC CTCAGAGTAT CCCTTATCAG AACCTTGGGC CCCTGGGCCC CTTCACTCAG 
TACTTGGTGG ACCACCATCA CACCCTCCTG TGCAATGGGT ATTGGCTTGC CTGGCTGATT 
CATGTGGGAG AGTCCTTGTA TGCCATAGTA TTGTGCAAGC AAAATGAGCT AAGAATACGG 
ATAAAGGTAT GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872169.1
CDS	380..697
Translation	MRGKPQQRSETLSKKKERKKIEAKNGDPNDCSEFLRSVWVVFWPQSIPYQNLGPLGPFTQYLVDHHHTLLCNGYWLAWLIHVGESLYAIVLCKQNELRIRIKVCE

Target ORF information:

RefSeq Version	XM_017016680.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 254 (TMEM254), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016680.2

ORF Insert Sequence:

ATGCGTGGAA AACCCCAGCA GCGGAGTGAG ACCCTGTCAA AAAAAAAAGA AAGAAAGAAA 
ATAGAAGCCA AGAATGGAGA CCCAAATGAC TGTTCAGAGT TTCTTCGGTC TGTGTGGGTT 
GTCTTCTGGC CTCAGAGTAT CCCTTATCAG AACCTTGGGC CCCTGGGCCC CTTCACTCAG 
TACTTGGTGG ACCACCATCA CACCCTCCTG TGCAATGGGT ATTGGCTTGC CTGGCTGATT 
CATGTGGGAG AGTCCTTGTA TGCCATAGTA TTGTGCAAGC AAAATGAGCT AAGAATACGG 
ATAAAGGTAT GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide association analysis of blood biomarkers in chronic obstructive pulmonary disease.
American journal of respiratory and critical care medicine186(12)1238-47(2012 Dec)
Kim DK,Cho MH,Hersh CP,Lomas DA,Miller BE,Kong X,Bakke P,Gulsvik A,Agust? A,Wouters E,Celli B,Coxson H,Vestbo J,MacNee W,Yates JC,Rennard S,Litonjua A,Qiu W,Beaty TH,Crapo JD,Riley JH,Tal-Singer R,Silverman EK,

A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease.
American journal of human genetics78(1)78-88(2006 Jan)
Grupe A,Li Y,Rowland C,Nowotny P,Hinrichs AL,Smemo S,Kauwe JS,Maxwell TJ,Cherny S,Doil L,Tacey K,van Luchene R,Myers A,Wavrant-De Vri?ze F,Kaleem M,Hollingworth P,Jehu L,Foy C,Archer N,Hamilton G,Holmans P,Morris CM,Catanese J,Sninsky J,White TJ,Powell J,Hardy J,O'Donovan M,Lovestone S,Jones L,Morris JC,Thal L,Owen M,Williams J,Goate A

Lineage-specific gene duplication and loss in human and great ape evolution.
PLoS biology2(7)E207(2004 Jul)
Fortna A,Kim Y,MacLaren E,Marshall K,Hahn G,Meltesen L,Brenton M,Hink R,Burgers S,Hernandez-Boussard T,Karimpour-Fard A,Glueck D,McGavran L,Berry R,Pollack J,Sikela JM

The DNA sequence and comparative analysis of human chromosome 10.
Nature429(6990)375-81(2004 May)
Deloukas P,Earthrowl ME,Grafham DV,Rubenfield M,French L,Steward CA,Sims SK,Jones MC,Searle S,Scott C,Howe K,Hunt SE,Andrews TD,Gilbert JG,Swarbreck D,Ashurst JL,Taylor A,Battles J,Bird CP,Ainscough R,Almeida JP,Ashwell RI,Ambrose KD,Babbage AK,Bagguley CL,Bailey J,Banerjee R,Bates K,Beasley H,Bray-Allen S,Brown AJ,Brown JY,Burford DC,Burrill W,Burton J,Cahill P,Camire D,Carter NP,Chapman JC,Clark SY,Clarke G,Clee CM,Clegg S,Corby N,Coulson A,Dhami P,Dutta I,Dunn M,Faulkner L,Frankish A,Frankland JA,Garner P,Garnett J,Gribble S,Griffiths C,Grocock R,Gustafson E,Hammond S,Harley JL,Hart E,Heath PD,Ho TP,Hopkins B,Horne J,Howden PJ,Huckle E,Hynds C,Johnson C,Johnson D,Kana A,Kay M,Kimberley AM,Kershaw JK,Kokkinaki M,Laird GK,Lawlor S,Lee HM,Leongamornlert DA,Laird G,Lloyd C,Lloyd DM,Loveland J,Lovell J,McLaren S,McLay KE,McMurray A,Mashreghi-Mohammadi M,Matthews L,Milne S,Nickerson T,Nguyen M,Overton-Larty E,Palmer SA,Pearce AV,Peck AI,Pelan S,Phillimore B,Porter K,Rice CM,Rogosin A,Ross MT,Sarafidou T,Sehra HK,Shownkeen R,Skuce CD,Smith M,Standring L,Sycamore N,Tester J,Thorpe A,Torcasso W,Tracey A,Tromans A,Tsolas J,Wall M,Walsh J,Wang H,Weinstock K,West AP,Willey DL,Whitehead SL,Wilming L,Wray PW,Young L,Chen Y,Lovering RC,Moschonas NK,Siebert R,Fechtel K,Bentley D,Durbin R,Hubbard T,Doucette-Stamm L,Beck S,Smith DR,Rogers J

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Gene Symbol	TMEM254
Entrez Gene ID	80195
Full Name	transmembrane protein 254
Synonyms	C10orf57,bA369J21.6
General protein information	Preferred Names transmembrane protein 254 Names transmembrane protein 254 transmembrane protein C10orf57
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q22.3

Homo sapiens (human)	TMEM254	NP_079401.2
	TMEM254	XP_001088642.1
Bos taurus (cattle)	TMEM254	NP_001068696.1
Danio rerio (zebrafish)	zgc:64043	NP_956664.1
Rattus norvegicus (Norway rat)	Fam213a	NP_001008298.1
Pan troglodytes (chimpanzee)	C10H10orf57	XP_003312694.1
Xenopus tropicalis (tropical clawed frog)	fam213a	NP_001011433.1

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5 or more Clone	50% OFF
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TMEM254 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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