PLEKHF2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLEKHF2
Entrez Gene ID	79666
Full Name	pleckstrin homology and FYVE domain containing 2
Synonyms	EAPF,PHAFIN2,ZFYVE18
General protein information	Preferred Names pleckstrin homology and FYVE domain containing 2 Names pleckstrin homology domain-containing family F member 2 PH and FYVE domain-containing protein 2 PH domain-containing family F member 2 endoplasmic reticulum-associated apoptosis-involved protein containing PH and FYVE domains pleckstrin homology domain containing, family F (with FYVE domain) member 2 zinc finger FYVE domain-containing protein 18
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q22.1
Disorder MIM:	615208

mRNA and Protein(s)

mRNA	Protein	Name
NM_024613.3	NP_078889.1	pleckstrin homology domain-containing family F member 2
NM_024613.4	NP_078889.1	pleckstrin homology domain-containing family F member 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PLEKHF2	XP_003311902.1
Mus musculus (house mouse)	Plekhf2	NP_780384.1
Xenopus tropicalis (tropical clawed frog)	plekhf2	NP_001016121.1
	PLEKHF2	XP_001090166.2
Drosophila melanogaster (fruit fly)	rush	NP_569923.2
Homo sapiens (human)	PLEKHF2	NP_078889.1
Rattus norvegicus (Norway rat)	Plekhf2	NP_001102125.1
Gallus gallus (chicken)	PLEKHF2	NP_001026118.1
Canis lupus familiaris (dog)	LOC102153293	XP_005638192.1
Danio rerio (zebrafish)	plekhf2	NP_956538.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010307	XP_314634.4
Caenorhabditis elegans (roundworm)	ZK632.12	NP_499183.1
Bos taurus (cattle)	PLEKHF2	NP_001091619.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural, thermodynamic, and phosphatidylinositol 3-phosphate binding properties of Phafin2.
Tang TX, Jo A, Deng J, Ellena JF, Lazar IM, Davis RM, Capelluto DG
Protein science : a publication of the Protein Society26(4)814-823(2017 Apr)

Lysosomal interaction of Akt with Phafin2: a critical step in the induction of autophagy.
Matsuda-Lennikov M, Suizu F, Hirata N, Hashimoto M, Kimura K, Nagamine T, Fujioka Y, Ohba Y, Iwanaga T, Noguchi M
PloS one9(1)e79795(2014)

The PtdIns3P-binding protein Phafin 2 mediates epidermal growth factor receptor degradation by promoting endosome fusion.
Pedersen NM, Raiborg C, Brech A, Skarpen E, Roxrud I, Platta HW, Liest?l K, Stenmark H
Traffic (Copenhagen, Denmark)13(11)1547-63(2012 Nov)

Next-generation sequencing to generate interactome datasets.
Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C, Svrzikapa N, Hirozane-Kishikawa T, Rietman E, Yang X, Sahalie J, Salehi-Ashtiani K, Hao T, Cusick ME, Hill DE, Roth FP, Braun P, Vidal M
Nature methods8(6)478-80(2011 Jun)

Radiation pharmacogenomics: a genome-wide association approach to identify radiation response biomarkers using human lymphoblastoid cell lines.
Niu N, Qin Y, Fridley BL, Hou J, Kalari KR, Zhu M, Wu TY, Jenkins GD, Batzler A, Wang L
Genome research20(11)1482-92(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PtdIns(3)P binding to Phafin2 occurs with high affinity, triggering minor conformational changes in the protein. Taken together, these studies represent a platform for establishing the structural basis of Phafin2 molecular interactions and the role of the two potentially redundant PtdIns(3)P-binding domains of the protein in endomembrane compartments.
Title: Structural, thermodynamic, and phosphatidylinositol 3-phosphate binding properties of Phafin2.

These findings establish that lysosomal accumulation of Akt and Phafin2 is a critical step in the induction of autophagy via an interaction with phosphatidylinositol 3 phosphate.
Title: Lysosomal interaction of Akt with Phafin2: a critical step in the induction of autophagy.

Phafin2 controls EGFR trafficking through early endosomes by facilitating endosome fusion in concert with EEA1.
Title: The PtdIns3P-binding protein Phafin 2 mediates epidermal growth factor receptor degradation by promoting endosome fusion.

These results provide a vivid example that an endosome modulator, such as Phafin2, may control the cells' responses to the extracellular cues.
Title: Phafin2 modulates the structure and function of endosomes by a Rab5-dependent mechanism.

Results demonstrate that EAPF/Phafin-2 facilitates TNF-alpha-induced cellular apoptosis through an ER-mitochondrial apoptotic pathway.
Title: EAPF/Phafin-2, a novel endoplasmic reticulum-associated protein, facilitates TNF-alpha-triggered cellular apoptosis through endoplasmic reticulum-mitochondrial apoptotic pathway.

The following PLEKHF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10514	NM_024613.4 Latest version!	Homo sapiens pleckstrin homology and FYVE domain containing 2 (PLEKHF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu10514	NM_024613.3	Homo sapiens pleckstrin homology and FYVE domain containing 2 (PLEKHF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLEKHF2 ( NM_024613.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_078889.1 Homo sapiens pleckstrin homology and FYVE domain containing 2 (PLEKHF2), mRNA.

Price & Availability↑

CloneID	OHu10514
Clone ID Related Accession (Same CDS sequence)	NM_024613.3 , NM_024613.4
Accession Version	NM_024613.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	pleckstrin homology domain-containing family F member 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018801.4, AL834473.1 and AA767886.1. On May 7, 2010 this sequence version replaced NM_024613.2. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.207233.1, SRR1660803.189498.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGATC GCTTGGCAAA CAGTGAAGCA AATACTAGAC GTATAAGTAT AGTGGAAAAC 
TGTTTTGGAG CAGCTGGTCA ACCTTTAACT ATACCTGGAC GAGTTCTTAT TGGAGAAGGA 
GTATTGACTA AGTTGTGCAG GAAAAAGCCC AAAGCAAGGC AGTTTTTCTT GTTTAATGAT 
ATTCTTGTAT ATGGCAATAT TGTCATCCAG AAGAAAAAAT ATAACAAACA ACATATTATT 
CCCCTGGAAA ATGTCACTAT TGATTCCATC AAAGATGAGG GAGACTTAAG GAATGGATGG 
CTAATCAAGA CACCAACTAA ATCTTTTGCA GTTTATGCTG CCACTGCTAC GGAGAAATCA 
GAATGGATGA ATCATATAAA TAAATGTGTT ACTGATTTAC TCTCCAAAAG TGGGAAGACA 
CCCAGTAATG AACATGCTGC TGTCTGGGTT CCTGACTCTG AGGCAACTGT ATGTATGCGT 
TGTCAGAAAG CAAAATTCAC ACCTGTTAAT CGTCGCCACC ATTGCCGCAA ATGTGGTTTT 
GTTGTCTGTG GGCCCTGCTC TGAAAAGAGA TTTCTTCTTC CCAGCCAGTC CTCTAAGCCT 
GTGCGGATTT GTGACTTCTG CTATGACCTG CTTTCTGCTG GGGACATGGC CACATGCCAG 
CCTGCTAGAT CAGACTCTTA CAGCCAGTCA TTGAAGTCTC CTTTAAATGA TATGTCTGAT 
GATGATGACG ATGATGATAG CAGTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078889.1
CDS	325..1074
Translation	MVDRLANSEANTRRISIVENCFGAAGQPLTIPGRVLIGEGVLTKLCRKKPKARQFFLFNDILVYGNIVIQKKKYNKQHIIPLENVTIDSIKDEGDLRNGWLIKTPTKSFAVYAATATEKSEWMNHINKCVTDLLSKSGKTPSNEHAAVWVPDSEATVCMRCQKAKFTPVNRRHHCRKCGFVVCGPCSEKRFLLPSQSSKPVRICDFCYDLLSAGDMATCQPARSDSYSQSLKSPLNDMSDDDDDDDSSD

Target ORF information:

RefSeq Version	NM_024613.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology and FYVE domain containing 2 (PLEKHF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024613.3

ORF Insert Sequence:

ATGGTGGATC GCTTGGCAAA CAGTGAAGCA AATACTAGAC GTATAAGTAT AGTGGAAAAC 
TGTTTTGGAG CAGCTGGTCA ACCTTTAACT ATACCTGGAC GAGTTCTTAT TGGAGAAGGA 
GTATTGACTA AGTTGTGCAG GAAAAAGCCC AAAGCAAGGC AGTTTTTCTT GTTTAATGAT 
ATTCTTGTAT ATGGCAATAT TGTCATCCAG AAGAAAAAAT ATAACAAACA ACATATTATT 
CCCCTGGAAA ATGTCACTAT TGATTCCATC AAAGATGAGG GAGACTTAAG GAATGGATGG 
CTAATCAAGA CACCAACTAA ATCTTTTGCA GTTTATGCTG CCACTGCTAC GGAGAAATCA 
GAATGGATGA ATCATATAAA TAAATGTGTT ACTGATTTAC TCTCCAAAAG TGGGAAGACA 
CCCAGTAATG AACATGCTGC TGTCTGGGTT CCTGACTCTG AGGCAACTGT ATGTATGCGT 
TGTCAGAAAG CAAAATTCAC ACCTGTTAAT CGTCGCCACC ATTGCCGCAA ATGTGGTTTT 
GTTGTCTGTG GGCCCTGCTC TGAAAAGAGA TTTCTTCTTC CCAGCCAGTC CTCTAAGCCT 
GTGCGGATTT GTGACTTCTG CTATGACCTG CTTTCTGCTG GGGACATGGC CACATGCCAG 
CCTGCTAGAT CAGACTCTTA CAGCCAGTCA TTGAAGTCTC CTTTAAATGA TATGTCTGAT 
GATGATGACG ATGATGATAG CAGTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHF2 ( NM_024613.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_078889.1 Homo sapiens pleckstrin homology and FYVE domain containing 2 (PLEKHF2), mRNA.

Price & Availability↑

CloneID	OHu10514
Clone ID Related Accession (Same CDS sequence)	NM_024613.3 , NM_024613.4
Accession Version	NM_024613.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	pleckstrin homology domain-containing family F member 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC018801.4, AL834473.1 and AA767886.1. On Nov 23, 2018 this sequence version replaced NM_024613.3. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.19888.1, SRR1660803.189498.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000315367.4/ ENSP00000322373.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGGATC GCTTGGCAAA CAGTGAAGCA AATACTAGAC GTATAAGTAT AGTGGAAAAC 
TGTTTTGGAG CAGCTGGTCA ACCTTTAACT ATACCTGGAC GAGTTCTTAT TGGAGAAGGA 
GTATTGACTA AGTTGTGCAG GAAAAAGCCC AAAGCAAGGC AGTTTTTCTT GTTTAATGAT 
ATTCTTGTAT ATGGCAATAT TGTCATCCAG AAGAAAAAAT ATAACAAACA ACATATTATT 
CCCCTGGAAA ATGTCACTAT TGATTCCATC AAAGATGAGG GAGACTTAAG GAATGGATGG 
CTAATCAAGA CACCAACTAA ATCTTTTGCA GTTTATGCTG CCACTGCTAC GGAGAAATCA 
GAATGGATGA ATCATATAAA TAAATGTGTT ACTGATTTAC TCTCCAAAAG TGGGAAGACA 
CCCAGTAATG AACATGCTGC TGTCTGGGTT CCTGACTCTG AGGCAACTGT ATGTATGCGT 
TGTCAGAAAG CAAAATTCAC ACCTGTTAAT CGTCGCCACC ATTGCCGCAA ATGTGGTTTT 
GTTGTCTGTG GGCCCTGCTC TGAAAAGAGA TTTCTTCTTC CCAGCCAGTC CTCTAAGCCT 
GTGCGGATTT GTGACTTCTG CTATGACCTG CTTTCTGCTG GGGACATGGC CACATGCCAG 
CCTGCTAGAT CAGACTCTTA CAGCCAGTCA TTGAAGTCTC CTTTAAATGA TATGTCTGAT 
GATGATGACG ATGATGATAG CAGTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078889.1
CDS	261..1010
Translation	MVDRLANSEANTRRISIVENCFGAAGQPLTIPGRVLIGEGVLTKLCRKKPKARQFFLFNDILVYGNIVIQKKKYNKQHIIPLENVTIDSIKDEGDLRNGWLIKTPTKSFAVYAATATEKSEWMNHINKCVTDLLSKSGKTPSNEHAAVWVPDSEATVCMRCQKAKFTPVNRRHHCRKCGFVVCGPCSEKRFLLPSQSSKPVRICDFCYDLLSAGDMATCQPARSDSYSQSLKSPLNDMSDDDDDDDSSD

Target ORF information:

RefSeq Version	NM_024613.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology and FYVE domain containing 2 (PLEKHF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024613.4

ORF Insert Sequence:

ATGGTGGATC GCTTGGCAAA CAGTGAAGCA AATACTAGAC GTATAAGTAT AGTGGAAAAC 
TGTTTTGGAG CAGCTGGTCA ACCTTTAACT ATACCTGGAC GAGTTCTTAT TGGAGAAGGA 
GTATTGACTA AGTTGTGCAG GAAAAAGCCC AAAGCAAGGC AGTTTTTCTT GTTTAATGAT 
ATTCTTGTAT ATGGCAATAT TGTCATCCAG AAGAAAAAAT ATAACAAACA ACATATTATT 
CCCCTGGAAA ATGTCACTAT TGATTCCATC AAAGATGAGG GAGACTTAAG GAATGGATGG 
CTAATCAAGA CACCAACTAA ATCTTTTGCA GTTTATGCTG CCACTGCTAC GGAGAAATCA 
GAATGGATGA ATCATATAAA TAAATGTGTT ACTGATTTAC TCTCCAAAAG TGGGAAGACA 
CCCAGTAATG AACATGCTGC TGTCTGGGTT CCTGACTCTG AGGCAACTGT ATGTATGCGT 
TGTCAGAAAG CAAAATTCAC ACCTGTTAAT CGTCGCCACC ATTGCCGCAA ATGTGGTTTT 
GTTGTCTGTG GGCCCTGCTC TGAAAAGAGA TTTCTTCTTC CCAGCCAGTC CTCTAAGCCT 
GTGCGGATTT GTGACTTCTG CTATGACCTG CTTTCTGCTG GGGACATGGC CACATGCCAG 
CCTGCTAGAT CAGACTCTTA CAGCCAGTCA TTGAAGTCTC CTTTAAATGA TATGTCTGAT 
GATGATGACG ATGATGATAG CAGTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural, thermodynamic, and phosphatidylinositol 3-phosphate binding properties of Phafin2.
Protein science : a publication of the Protein Society26(4)814-823(2017 Apr)
Tang TX,Jo A,Deng J,Ellena JF,Lazar IM,Davis RM,Capelluto DG

Lysosomal interaction of Akt with Phafin2: a critical step in the induction of autophagy.
PloS one9(1)e79795(2014)
Matsuda-Lennikov M,Suizu F,Hirata N,Hashimoto M,Kimura K,Nagamine T,Fujioka Y,Ohba Y,Iwanaga T,Noguchi M

The PtdIns3P-binding protein Phafin 2 mediates epidermal growth factor receptor degradation by promoting endosome fusion.
Traffic (Copenhagen, Denmark)13(11)1547-63(2012 Nov)
Pedersen NM,Raiborg C,Brech A,Skarpen E,Roxrud I,Platta HW,Liest?l K,Stenmark H

Next-generation sequencing to generate interactome datasets.
Nature methods8(6)478-80(2011 Jun)
Yu H,Tardivo L,Tam S,Weiner E,Gebreab F,Fan C,Svrzikapa N,Hirozane-Kishikawa T,Rietman E,Yang X,Sahalie J,Salehi-Ashtiani K,Hao T,Cusick ME,Hill DE,Roth FP,Braun P,Vidal M

Radiation pharmacogenomics: a genome-wide association approach to identify radiation response biomarkers using human lymphoblastoid cell lines.
Genome research20(11)1482-92(2010 Nov)
Niu N,Qin Y,Fridley BL,Hou J,Kalari KR,Zhu M,Wu TY,Jenkins GD,Batzler A,Wang L