ZNF224 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZNF224
Entrez Gene ID	7767
Full Name	zinc finger protein 224
Synonyms	BMZF-2,BMZF2,KOX22,ZNF233,ZNF255,ZNF27
General protein information	Preferred Names zinc finger protein 224 Names zinc finger protein 224 bone marrow zinc finger 2 zinc finger protein 233 zinc finger protein 255 zinc finger protein 27 zinc finger protein KOX22
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.31
Summary	This gene encodes a member of the Krueppel C2H2-type zinc-finger family of proteins. The encoded protein represses transcription of the aldolase A gene, which encodes a key enzyme in glycolysis. The encoded zinc-finger protein may also function as a transcriptional co-activator with Wilms' tumor protein 1 to regulate apoptotic genes in leukemia. [provided by RefSeq, Jul 2016].
Disorder MIM:	194555

mRNA and Protein(s)

mRNA	Protein	Name
NM_001321645.1	NP_001308574.1	zinc finger protein 224
NM_013398.3	NP_037530.2	zinc finger protein 224
XM_005259221.2	XP_005259278.1	zinc finger protein 224 isoform X1
NM_013398.2	NP_037530.2	zinc finger protein 224
XM_017027261.1	XP_016882750.1	zinc finger protein 224 isoform X1
XM_017027261.2	XP_016882750.1	zinc finger protein 224 isoform X1
NM_001321645.2	NP_001308574.1	zinc finger protein 224
NM_013398.4	NP_037530.2	zinc finger protein 224

Pathways from BioSystems

Homologies

Homo sapiens (human)	ZNF224	NP_037530.2
	LOC100424339	XP_002801318.1
Pan troglodytes (chimpanzee)	ZNF224	XP_003339371.1

Gene Ontology
Bibliography

Related articles in PubMed

The Complex Role of the ZNF224 Transcription Factor in Cancer.
Cesaro E, Sodaro G, Montano G, Grosso M, Lupo A, Costanzo P
Advances in protein chemistry and structural biology107191-222(2017)

Role of ZNF224 in cell growth and chemoresistance of chronic lymphocitic leukemia.
Busiello T, Ciano M, Romano S, Sodaro G, Garofalo O, Bruzzese D, Simeone L, Chiurazzi F, Fiammetta Romano M, Costanzo P, Cesaro E
Human molecular genetics26(2)344-353(2017 01)

ZNF224, Kr?ppel like zinc finger protein, induces cell growth and apoptosis-resistance by down-regulation of p21 and p53 via miR-663a.
Cho JG, Park S, Lim CH, Kim HS, Song SY, Roh TY, Sung JH, Suh W, Ham SJ, Lim KH, Park SG
Oncotarget7(21)31177-90(2016 May)

The hematopoietic tumor suppressor interferon regulatory factor 8 (IRF8) is upregulated by the antimetabolite cytarabine in leukemic cells involving the zinc finger protein ZNF224, acting as a cofactor of the Wilms' tumor gene 1 (WT1) protein.
Montano G, Ullmark T, Jernmark-Nilsson H, Sodaro G, Drott K, Costanzo P, Vidovic K, Gullberg U
Leukemia research4060-7(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increasing information on the mechanism through which ZNF224 can operate could lead to the identification of agents capable of modulating ZNF224 function, thus potentially paving the way to new therapeutic strategies for treatment of cancer
Title: The Complex Role of the ZNF224 Transcription Factor in Cancer.

we showed that ZNF224 positively modulates cyclin D3 gene expression. Consistently, we observed that alteration of ZNF224 expression leads to defects in cell cycle control. All together, our results strongly suggest that in Chronic lymphocytic leukaemia (CLL)cells high expression level of ZNF224 can lead to inappropriate cell growth and apoptosis resistance, thus contributing to CLL progression
Title: Role of ZNF224 in cell growth and chemoresistance of chronic lymphocitic leukemia.

The analyses using human breast ductal carcinoma tissues exhibited that the expression of ZNF224 and miR-663a was increased in cancer compared to non-cancer region.
Title: ZNF224, Kr?ppel like zinc finger protein, induces cell growth and apoptosis-resistance by down-regulation of p21 and p53 via miR-663a.

Data show that Wilms' tumor 1 protein (WT1)binds to the zinc finger protein 224 (ZNF224) promoter and represses ZNF224 expression.
Title: WT1-mediated repression of the proapoptotic transcription factor ZNF224 is triggered by the BCR-ABL oncogene.

ZNF224 acts in fine tuning of WT1-dependent control of gene expression, acting as a co-activator of WT1 in the regulation of proapoptotic genes and suppressing WT1 mediated transactivation of antiapoptotitc genes.
Title: Role of WT1-ZNF224 interaction in the expression of apoptosis-regulating genes.

The following ZNF224 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZNF224 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10433	NM_013398.4 Latest version!	Homo sapiens zinc finger protein 224 (ZNF224), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu10433	NM_001321645.2 Latest version!	Homo sapiens zinc finger protein 224 (ZNF224), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu10433	XM_005259221.2 Latest version!	Homo sapiens zinc finger protein 224 (ZNF224), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu10433	XM_017027261.2 Latest version!	Homo sapiens zinc finger protein 224 (ZNF224), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu10433	NM_013398.2	Homo sapiens zinc finger protein 224 (ZNF224), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu10433	XM_017027261.1	Homo sapiens zinc finger protein 224 (ZNF224), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu10433	NM_001321645.1	Homo sapiens zinc finger protein 224 (ZNF224), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu10433	NM_013398.3	Homo sapiens zinc finger protein 224 (ZNF224), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZNF224 ( NM_001321645.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308574.1 Homo sapiens zinc finger protein 224 (ZNF224), mRNA.

Price & Availability↑

CloneID	OHu10433
Clone ID Related Accession (Same CDS sequence)	NM_001321645.1 , NM_013398.3 , XM_005259221.2 , NM_013398.2 , XM_017027261.1 , XM_017027261.2 , NM_001321645.2 , NM_013398.4
Accession Version	NM_001321645.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-01
Organism	Homo sapiens(human)
Product	zinc finger protein 224
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA245440.1, DB228337.1, CN304667.1, AC114266.2, AC021092.1 and AA554187.1. On Mar 29, 2016 this sequence version replaced XM_005259221.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.250708.1, SRR1660809.190731.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308574.1
CDS	360..2483
Translation	MTTFKEAMTFKDVAVVFTEEELGLLDLAQRKLYRDVMLENFRNLLSVGHQAFHRDTFHFLREEKIWMMKTAIQREGNSGDKIQTEMETVSEAGTHQEWSFQQIWEKIASDLTRSQDLMINSSQFSKEGDFPCQTEAGLSVIHTRQKSSQGNGYKPSFSDVSHFDFHQQLHSGEKSHTCDECGKNFCYISALRIHQRVHMGEKCYKCDVCGKEFSQSSHLQTHQRVHTGEKPFKCVECGKGFSRRSALNVHHKLHTGEKPYNCEECGKAFIHDSQLQEHQRIHTGEKPFKCDICGKSFCGRSRLNRHSMVHTAEKPFRCDTCDKSFRQRSALNSHRMIHTGEKPYKCEECGKGFICRRDLYTHHMVHTGEKPYNCKECGKSFRWASCLLKHQRVHSGEKPFKCEECGKGFYTNSQCYSHQRSHSGEKPYKCVECGKGYKRRLDLDFHQRVHTGEKLYNCKECGKSFSRAPCLLKHERLHSGEKPFQCEECGKRFTQNSHLHSHQRVHTGEKPYKCEKCGKGYNSKFNLDMHQKVHTGERPYNCKECGKSFGWASCLLKHQRLHSGEKPFKCEECGKRFTQNSQLHSHQRVHTGEKPYKCDECGKGFSWSSTRLTHQRRHSRETPLKCEQHGKNIVQNSFSKVQEKVHSVEKPYKCEDCGKGYNRRLNLDMHQRVHMGEKTWKCRECDMCFSQASSLRLHQNVHVGEKP

Target ORF information:

RefSeq Version	NM_001321645.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 224 (ZNF224), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321645.1

ORF Insert Sequence:

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF224 ( NM_013398.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_037530.2 Homo sapiens zinc finger protein 224 (ZNF224), mRNA.

Price & Availability↑

CloneID	OHu10433
Clone ID Related Accession (Same CDS sequence)	NM_001321645.1 , NM_013398.3 , XM_005259221.2 , NM_013398.2 , XM_017027261.1 , XM_017027261.2 , NM_001321645.2 , NM_013398.4
Accession Version	NM_013398.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-01
Organism	Homo sapiens(human)
Product	zinc finger protein 224
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA245440.1, BC051902.1, AC114266.2, AC021092.1 and AA554187.1. This sequence is a reference standard in the RefSeqGene project. On Mar 29, 2016 this sequence version replaced NM_013398.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA245440.1, SRR1660803.251412.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037530.2
CDS	318..2441
Translation	MTTFKEAMTFKDVAVVFTEEELGLLDLAQRKLYRDVMLENFRNLLSVGHQAFHRDTFHFLREEKIWMMKTAIQREGNSGDKIQTEMETVSEAGTHQEWSFQQIWEKIASDLTRSQDLMINSSQFSKEGDFPCQTEAGLSVIHTRQKSSQGNGYKPSFSDVSHFDFHQQLHSGEKSHTCDECGKNFCYISALRIHQRVHMGEKCYKCDVCGKEFSQSSHLQTHQRVHTGEKPFKCVECGKGFSRRSALNVHHKLHTGEKPYNCEECGKAFIHDSQLQEHQRIHTGEKPFKCDICGKSFCGRSRLNRHSMVHTAEKPFRCDTCDKSFRQRSALNSHRMIHTGEKPYKCEECGKGFICRRDLYTHHMVHTGEKPYNCKECGKSFRWASCLLKHQRVHSGEKPFKCEECGKGFYTNSQCYSHQRSHSGEKPYKCVECGKGYKRRLDLDFHQRVHTGEKLYNCKECGKSFSRAPCLLKHERLHSGEKPFQCEECGKRFTQNSHLHSHQRVHTGEKPYKCEKCGKGYNSKFNLDMHQKVHTGERPYNCKECGKSFGWASCLLKHQRLHSGEKPFKCEECGKRFTQNSQLHSHQRVHTGEKPYKCDECGKGFSWSSTRLTHQRRHSRETPLKCEQHGKNIVQNSFSKVQEKVHSVEKPYKCEDCGKGYNRRLNLDMHQRVHMGEKTWKCRECDMCFSQASSLRLHQNVHVGEKP

Target ORF information:

RefSeq Version	NM_013398.3
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 224 (ZNF224), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013398.3

ORF Insert Sequence:

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF224 ( NM_013398.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_037530.2 Homo sapiens zinc finger protein 224 (ZNF224), mRNA.

Price & Availability↑

CloneID	OHu10433
Clone ID Related Accession (Same CDS sequence)	NM_001321645.1 , NM_013398.3 , XM_005259221.2 , NM_013398.2 , XM_017027261.1 , XM_017027261.2 , NM_001321645.2 , NM_013398.4
Accession Version	NM_013398.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	zinc finger protein 224
Comment

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_037530.2

Translation

MTTFKEAMTFKDVAVVFTEEELGLLDLAQRKLYRDVMLENFRNLLSVGHQAFHRDTFHFLREEKIWMMKTAIQREGNSGDKIQTEMETVSEAGTHQEWSFQQIWEKIASDLTRSQDLMINSSQFSKEGDFPCQTEAGLSVIHTRQKSSQGNGYKPSFSDVSHFDFHQQLHSGEKSHTCDECGKNFCYISALRIHQRVHMGEKCYKCDVCGKEFSQSSHLQTHQRVHTGEKPFKCVECGKGFSRRSALNVHHKLHTGEKPYNCEECGKAFIHDSQLQEHQRIHTGEKPFKCDICGKSFCGRSRLNRHSMVHTAEKPFRCDTCDKSFRQRSALNSHRMIHTGEKPYKCEECGKGFICRRDLYTHHMVHTGEKPYNCKECGKSFRWASCLLKHQRVHSGEKPFKCEECGKGFYTNSQCYSHQRSHSGEKPYKCVECGKGYKRRLDLDFHQRVHTGEKLYNCKECGKSFSRAPCLLKHERLHSGEKPFQCEECGKRFTQNSHLHSHQRVHTGEKPYKCEKCGKGYNSKFNLDMHQKVHTGERPYNCKECGKSFGWASCLLKHQRLHSGEKPFKCEECGKRFTQNSQLHSHQRVHTGEKPYKCDECGKGFSWSSTRLTHQRRHSRETPLKCEQHGKNIVQNSFSKVQEKVHSVEKPYKCEDCGKGYNRRLNLDMHQRVHMGEKTWKCRECDMCFSQASSLRLHQNVHVGEKP

Target ORF information:

RefSeq Version	NM_013398.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens zinc finger protein 224 (ZNF224), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013398.2

ORF Insert Sequence:

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF224 ( NM_001321645.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308574.1 Homo sapiens zinc finger protein 224 (ZNF224), mRNA.

Price & Availability↑

CloneID	OHu10433
Clone ID Related Accession (Same CDS sequence)	NM_001321645.1 , NM_013398.3 , XM_005259221.2 , NM_013398.2 , XM_017027261.1 , XM_017027261.2 , NM_001321645.2 , NM_013398.4
Accession Version	NM_001321645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	zinc finger protein 224
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA245440.1, DB228337.1, CN304667.1, AC114266.2, AC021092.1 and AA554187.1. On Jun 2, 2019 this sequence version replaced NM_001321645.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.249299.1, SRR1660809.250708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308574.1
CDS	328..2451
Translation	MTTFKEAMTFKDVAVVFTEEELGLLDLAQRKLYRDVMLENFRNLLSVGHQAFHRDTFHFLREEKIWMMKTAIQREGNSGDKIQTEMETVSEAGTHQEWSFQQIWEKIASDLTRSQDLMINSSQFSKEGDFPCQTEAGLSVIHTRQKSSQGNGYKPSFSDVSHFDFHQQLHSGEKSHTCDECGKNFCYISALRIHQRVHMGEKCYKCDVCGKEFSQSSHLQTHQRVHTGEKPFKCVECGKGFSRRSALNVHHKLHTGEKPYNCEECGKAFIHDSQLQEHQRIHTGEKPFKCDICGKSFCGRSRLNRHSMVHTAEKPFRCDTCDKSFRQRSALNSHRMIHTGEKPYKCEECGKGFICRRDLYTHHMVHTGEKPYNCKECGKSFRWASCLLKHQRVHSGEKPFKCEECGKGFYTNSQCYSHQRSHSGEKPYKCVECGKGYKRRLDLDFHQRVHTGEKLYNCKECGKSFSRAPCLLKHERLHSGEKPFQCEECGKRFTQNSHLHSHQRVHTGEKPYKCEKCGKGYNSKFNLDMHQKVHTGERPYNCKECGKSFGWASCLLKHQRLHSGEKPFKCEECGKRFTQNSQLHSHQRVHTGEKPYKCDECGKGFSWSSTRLTHQRRHSRETPLKCEQHGKNIVQNSFSKVQEKVHSVEKPYKCEDCGKGYNRRLNLDMHQRVHMGEKTWKCRECDMCFSQASSLRLHQNVHVGEKP

Target ORF information:

RefSeq Version	NM_001321645.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 224 (ZNF224), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321645.2

ORF Insert Sequence:

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF224 ( NM_013398.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_037530.2 Homo sapiens zinc finger protein 224 (ZNF224), mRNA.

Price & Availability↑

CloneID	OHu10433
Clone ID Related Accession (Same CDS sequence)	NM_001321645.1 , NM_013398.3 , XM_005259221.2 , NM_013398.2 , XM_017027261.1 , XM_017027261.2 , NM_001321645.2 , NM_013398.4
Accession Version	NM_013398.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	zinc finger protein 224
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA245440.1, BC051902.1, AC114266.2, AC021092.1 and AA554187.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_013398.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.200276.1, SRR1803614.173730.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037530.2
CDS	286..2409
Translation	MTTFKEAMTFKDVAVVFTEEELGLLDLAQRKLYRDVMLENFRNLLSVGHQAFHRDTFHFLREEKIWMMKTAIQREGNSGDKIQTEMETVSEAGTHQEWSFQQIWEKIASDLTRSQDLMINSSQFSKEGDFPCQTEAGLSVIHTRQKSSQGNGYKPSFSDVSHFDFHQQLHSGEKSHTCDECGKNFCYISALRIHQRVHMGEKCYKCDVCGKEFSQSSHLQTHQRVHTGEKPFKCVECGKGFSRRSALNVHHKLHTGEKPYNCEECGKAFIHDSQLQEHQRIHTGEKPFKCDICGKSFCGRSRLNRHSMVHTAEKPFRCDTCDKSFRQRSALNSHRMIHTGEKPYKCEECGKGFICRRDLYTHHMVHTGEKPYNCKECGKSFRWASCLLKHQRVHSGEKPFKCEECGKGFYTNSQCYSHQRSHSGEKPYKCVECGKGYKRRLDLDFHQRVHTGEKLYNCKECGKSFSRAPCLLKHERLHSGEKPFQCEECGKRFTQNSHLHSHQRVHTGEKPYKCEKCGKGYNSKFNLDMHQKVHTGERPYNCKECGKSFGWASCLLKHQRLHSGEKPFKCEECGKRFTQNSQLHSHQRVHTGEKPYKCDECGKGFSWSSTRLTHQRRHSRETPLKCEQHGKNIVQNSFSKVQEKVHSVEKPYKCEDCGKGYNRRLNLDMHQRVHMGEKTWKCRECDMCFSQASSLRLHQNVHVGEKP

Target ORF information:

RefSeq Version	NM_013398.4
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 224 (ZNF224), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013398.4

ORF Insert Sequence:

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF224 ( XM_005259221.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005259278.1 Homo sapiens zinc finger protein 224 (ZNF224), transcript variant X1, mRNA.

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CloneID	OHu10433
Clone ID Related Accession (Same CDS sequence)	NM_001321645.1 , NM_013398.3 , XM_005259221.2 , NM_013398.2 , XM_017027261.1 , XM_017027261.2 , NM_001321645.2 , NM_013398.4
Accession Version	XM_005259221.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	zinc finger protein 224 isoform X1
Comment

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005259278.1

Translation

MTTFKEAMTFKDVAVVFTEEELGLLDLAQRKLYRDVMLENFRNLLSVGHQAFHRDTFHFLREEKIWMMKTAIQREGNSGDKIQTEMETVSEAGTHQEWSFQQIWEKIASDLTRSQDLMINSSQFSKEGDFPCQTEAGLSVIHTRQKSSQGNGYKPSFSDVSHFDFHQQLHSGEKSHTCDECGKNFCYISALRIHQRVHMGEKCYKCDVCGKEFSQSSHLQTHQRVHTGEKPFKCVECGKGFSRRSALNVHHKLHTGEKPYNCEECGKAFIHDSQLQEHQRIHTGEKPFKCDICGKSFCGRSRLNRHSMVHTAEKPFRCDTCDKSFRQRSALNSHRMIHTGEKPYKCEECGKGFICRRDLYTHHMVHTGEKPYNCKECGKSFRWASCLLKHQRVHSGEKPFKCEECGKGFYTNSQCYSHQRSHSGEKPYKCVECGKGYKRRLDLDFHQRVHTGEKLYNCKECGKSFSRAPCLLKHERLHSGEKPFQCEECGKRFTQNSHLHSHQRVHTGEKPYKCEKCGKGYNSKFNLDMHQKVHTGERPYNCKECGKSFGWASCLLKHQRLHSGEKPFKCEECGKRFTQNSQLHSHQRVHTGEKPYKCDECGKGFSWSSTRLTHQRRHSRETPLKCEQHGKNIVQNSFSKVQEKVHSVEKPYKCEDCGKGYNRRLNLDMHQRVHMGEKTWKCRECDMCFSQASSLRLHQNVHVGEKP

Target ORF information:

RefSeq Version	XM_005259221.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens zinc finger protein 224 (ZNF224), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005259221.2

ORF Insert Sequence:

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF224 ( XM_017027261.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882750.1 Homo sapiens zinc finger protein 224 (ZNF224), transcript variant X1, mRNA.

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CloneID	OHu10433
Clone ID Related Accession (Same CDS sequence)	NM_001321645.1 , NM_013398.3 , XM_005259221.2 , NM_013398.2 , XM_017027261.1 , XM_017027261.2 , NM_001321645.2 , NM_013398.4
Accession Version	XM_017027261.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	zinc finger protein 224 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882750.1
CDS	394..2517
Translation	MTTFKEAMTFKDVAVVFTEEELGLLDLAQRKLYRDVMLENFRNLLSVGHQAFHRDTFHFLREEKIWMMKTAIQREGNSGDKIQTEMETVSEAGTHQEWSFQQIWEKIASDLTRSQDLMINSSQFSKEGDFPCQTEAGLSVIHTRQKSSQGNGYKPSFSDVSHFDFHQQLHSGEKSHTCDECGKNFCYISALRIHQRVHMGEKCYKCDVCGKEFSQSSHLQTHQRVHTGEKPFKCVECGKGFSRRSALNVHHKLHTGEKPYNCEECGKAFIHDSQLQEHQRIHTGEKPFKCDICGKSFCGRSRLNRHSMVHTAEKPFRCDTCDKSFRQRSALNSHRMIHTGEKPYKCEECGKGFICRRDLYTHHMVHTGEKPYNCKECGKSFRWASCLLKHQRVHSGEKPFKCEECGKGFYTNSQCYSHQRSHSGEKPYKCVECGKGYKRRLDLDFHQRVHTGEKLYNCKECGKSFSRAPCLLKHERLHSGEKPFQCEECGKRFTQNSHLHSHQRVHTGEKPYKCEKCGKGYNSKFNLDMHQKVHTGERPYNCKECGKSFGWASCLLKHQRLHSGEKPFKCEECGKRFTQNSQLHSHQRVHTGEKPYKCDECGKGFSWSSTRLTHQRRHSRETPLKCEQHGKNIVQNSFSKVQEKVHSVEKPYKCEDCGKGYNRRLNLDMHQRVHMGEKTWKCRECDMCFSQASSLRLHQNVHVGEKP

Target ORF information:

RefSeq Version	XM_017027261.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 224 (ZNF224), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027261.1

ORF Insert Sequence:

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF224 ( XM_017027261.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882750.1 Homo sapiens zinc finger protein 224 (ZNF224), transcript variant X1, mRNA.

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CloneID	OHu10433
Clone ID Related Accession (Same CDS sequence)	NM_001321645.1 , NM_013398.3 , XM_005259221.2 , NM_013398.2 , XM_017027261.1 , XM_017027261.2 , NM_001321645.2 , NM_013398.4
Accession Version	XM_017027261.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	zinc finger protein 224 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017027261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882750.1
CDS	2151..4274
Translation	MTTFKEAMTFKDVAVVFTEEELGLLDLAQRKLYRDVMLENFRNLLSVGHQAFHRDTFHFLREEKIWMMKTAIQREGNSGDKIQTEMETVSEAGTHQEWSFQQIWEKIASDLTRSQDLMINSSQFSKEGDFPCQTEAGLSVIHTRQKSSQGNGYKPSFSDVSHFDFHQQLHSGEKSHTCDECGKNFCYISALRIHQRVHMGEKCYKCDVCGKEFSQSSHLQTHQRVHTGEKPFKCVECGKGFSRRSALNVHHKLHTGEKPYNCEECGKAFIHDSQLQEHQRIHTGEKPFKCDICGKSFCGRSRLNRHSMVHTAEKPFRCDTCDKSFRQRSALNSHRMIHTGEKPYKCEECGKGFICRRDLYTHHMVHTGEKPYNCKECGKSFRWASCLLKHQRVHSGEKPFKCEECGKGFYTNSQCYSHQRSHSGEKPYKCVECGKGYKRRLDLDFHQRVHTGEKLYNCKECGKSFSRAPCLLKHERLHSGEKPFQCEECGKRFTQNSHLHSHQRVHTGEKPYKCEKCGKGYNSKFNLDMHQKVHTGERPYNCKECGKSFGWASCLLKHQRLHSGEKPFKCEECGKRFTQNSQLHSHQRVHTGEKPYKCDECGKGFSWSSTRLTHQRRHSRETPLKCEQHGKNIVQNSFSKVQEKVHSVEKPYKCEDCGKGYNRRLNLDMHQRVHMGEKTWKCRECDMCFSQASSLRLHQNVHVGEKP

Target ORF information:

RefSeq Version	XM_017027261.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 224 (ZNF224), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027261.2

ORF Insert Sequence:

ATGACCACGT TCAAGGAGGC AATGACCTTC AAGGACGTGG CTGTGGTCTT CACTGAGGAA 
GAGCTGGGGC TGCTGGACCT TGCTCAGAGG AAGCTGTATC GAGATGTGAT GCTGGAGAAC 
TTCAGGAACC TGCTCTCAGT GGGACATCAA GCATTCCACA GGGATACTTT CCACTTCCTA 
AGGGAAGAAA AGATTTGGAT GATGAAGACA GCAATCCAAA GGGAAGGGAA TTCAGGAGAC 
AAGATCCAAA CTGAGATGGA GACTGTTTCA GAAGCAGGAA CACATCAAGA GTGGTCCTTC 
CAGCAAATCT GGGAAAAAAT TGCAAGTGAT TTAACCAGGT CTCAAGACTT GATGATAAAT 
AGCTCTCAGT TCTCCAAAGA AGGTGATTTC CCCTGCCAGA CTGAGGCAGG ACTATCTGTA 
ATTCACACAA GACAGAAATC TTCCCAGGGC AATGGATATA AACCATCCTT CAGTGATGTC 
TCCCACTTTG ATTTTCATCA ACAATTACAC TCAGGAGAGA AATCTCATAC GTGTGATGAG 
TGTGGAAAGA ACTTTTGTTA CATCTCAGCC CTTCGTATTC ATCAGAGAGT CCACATGGGA 
GAGAAATGCT ATAAGTGTGA CGTGTGTGGT AAGGAATTCA GTCAGAGTTC ACATCTGCAA 
ACTCATCAGA GAGTCCACAC TGGAGAGAAA CCGTTCAAAT GTGTGGAATG TGGGAAAGGC 
TTCAGTCGTA GATCAGCACT TAATGTTCAT CATAAATTAC ACACAGGAGA GAAACCTTAT 
AATTGTGAGG AATGCGGGAA GGCCTTCATT CACGATTCCC AGCTTCAAGA ACATCAGAGA 
ATCCATACGG GGGAGAAGCC ATTCAAATGT GATATATGTG GTAAGAGCTT CTGTGGTAGA 
TCAAGACTTA ATAGGCATTC CATGGTTCAC ACGGCAGAGA AACCATTCCG ATGTGATACG 
TGTGATAAGA GCTTTCGTCA GAGATCAGCA CTTAATAGTC ATCGCATGAT CCACACAGGA 
GAGAAACCAT ACAAATGTGA GGAGTGTGGA AAAGGCTTTA TTTGTAGGCG AGATCTTTAT 
ACGCATCATA TGGTCCACAC GGGAGAAAAG CCATATAATT GTAAAGAGTG TGGGAAGAGC 
TTCAGATGGG CCTCGTGTCT TTTGAAACAT CAGCGAGTCC ACAGTGGAGA AAAACCATTC 
AAATGTGAAG AATGTGGGAA AGGATTTTAC ACAAATTCAC AATGCTATTC CCACCAGAGA 
TCCCATAGTG GAGAAAAACC ATACAAATGT GTGGAGTGTG GGAAGGGCTA CAAAAGGAGG 
TTGGATCTTG ACTTTCACCA GCGCGTCCAT ACAGGAGAGA AACTGTATAA TTGTAAGGAA 
TGTGGGAAGA GCTTTAGTCG GGCCCCATGT CTTTTGAAAC ATGAGAGACT CCACAGTGGA 
GAAAAACCAT TCCAATGTGA AGAGTGTGGG AAGAGATTTA CTCAAAATTC ACATCTTCAT 
TCCCATCAGA GAGTTCACAC TGGAGAAAAG CCATACAAAT GTGAGAAGTG TGGAAAGGGC 
TACAATAGTA AGTTTAATCT TGATATGCAC CAGAAGGTCC ACACAGGAGA GAGACCATAC 
AATTGTAAGG AATGTGGGAA GAGTTTTGGC TGGGCCTCGT GTCTTTTGAA ACATCAGAGA 
CTGCACAGTG GAGAAAAACC ATTCAAATGT GAAGAGTGTG GGAAAAGATT TACTCAGAAT 
TCACAGCTTC ATTCTCATCA AAGAGTGCAC ACTGGAGAAA AGCCATACAA ATGTGATGAG 
TGTGGGAAGG GCTTCAGCTG GTCCTCAACT CGTCTGACCC ATCAGAGACG CCACAGCAGA 
GAAACACCTC TCAAATGTGA GCAGCATGGG AAGAACATTG TACAGAATTC ATTCTCTAAA 
GTGCAAGAAA AAGTTCACAG TGTAGAAAAG CCATACAAAT GTGAGGACTG TGGGAAGGGC 
TACAACAGGC GCTTGAATCT TGATATGCAT CAGAGGGTCC ACATGGGAGA GAAAACATGG 
AAGTGTAGGG AGTGTGATAT GTGCTTTAGT CAGGCCTCAA GCCTTCGACT TCATCAGAAT 
GTTCATGTTG GAGAAAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Complex Role of the ZNF224 Transcription Factor in Cancer.
Advances in protein chemistry and structural biology107191-222(2017)
Cesaro E,Sodaro G,Montano G,Grosso M,Lupo A,Costanzo P

Role of ZNF224 in cell growth and chemoresistance of chronic lymphocitic leukemia.
Human molecular genetics26(2)344-353(2017 01)
Busiello T,Ciano M,Romano S,Sodaro G,Garofalo O,Bruzzese D,Simeone L,Chiurazzi F,Fiammetta Romano M,Costanzo P,Cesaro E

ZNF224, Kr?ppel like zinc finger protein, induces cell growth and apoptosis-resistance by down-regulation of p21 and p53 via miR-663a.
Oncotarget7(21)31177-90(2016 May)
Cho JG,Park S,Lim CH,Kim HS,Song SY,Roh TY,Sung JH,Suh W,Ham SJ,Lim KH,Park SG

The hematopoietic tumor suppressor interferon regulatory factor 8 (IRF8) is upregulated by the antimetabolite cytarabine in leukemic cells involving the zinc finger protein ZNF224, acting as a cofactor of the Wilms' tumor gene 1 (WT1) protein.
Leukemia research4060-7(2016 Jan)
Montano G,Ullmark T,Jernmark-Nilsson H,Sodaro G,Drott K,Costanzo P,Vidovic K,Gullberg U

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ZNF224 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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