VIL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VIL1
Entrez Gene ID	7429
Full Name	villin 1
Synonyms	D2S1471,VIL
General protein information	Preferred Names villin 1 Names villin-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q35
Summary	This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008].
Disorder MIM:	193040
Disorder Html:	Cholestasis, progressive canalicular (1)

mRNA and Protein(s)

mRNA	Protein	Name
NM_007127.2	NP_009058.2	villin-1
NM_007127.3	NP_009058.2	villin-1

Pathways from BioSystems

Homologies

	VIL1	XP_001090524.1
Bos taurus (cattle)	VIL1	NP_001013609.1
Mus musculus (house mouse)	Vil1	NP_033535.2
Arabidopsis thaliana (thale cress)	AT5G57320	NP_200542.1
Arabidopsis thaliana (thale cress)	VLN4	NP_194745.1
Xenopus tropicalis (tropical clawed frog)	vil1	NP_001096367.1
Gallus gallus (chicken)	VIL1	NP_990773.1
Homo sapiens (human)	VIL1	NP_009058.2
Pan troglodytes (chimpanzee)	VIL1	XP_001157250.2
Danio rerio (zebrafish)	vil1	NP_956532.1
Oryza sativa (rice)	Os08g0240800	NP_001061335.1
Oryza sativa (rice)	Os06g0659300	NP_001058263.1
Canis lupus familiaris (dog)	VIL1	XP_545642.2
Rattus norvegicus (Norway rat)	Vil1	NP_001101694.1
Oryza sativa (rice)	Os04g0604000	NP_001053784.1

Gene Ontology
Bibliography

Related articles in PubMed

Villin-1 and Gelsolin Regulate Changes in Actin Dynamics That Affect Cell Survival Signaling Pathways and Intestinal Inflammation.
Roy S, Esmaeilniakooshkghazi A, Patnaik S, Wang Y, George SP, Ahrorov A, Hou JK, Herron AJ, Sesaki H, Khurana S
Gastroenterology(2017 Dec)

Plasmatic Villin 1 Is a Novel In Vivo Marker of Proximal Tubular Cell Injury During Renal Ischemia-Reperfusion.
Decuypere JP, Ceulemans LJ, Wylin T, Martinet W, Monbaliu D, Pirenne J, Jochmans I
Transplantation101(11)e330-e336(2017 Nov)

Clinicopathological, radiographic, and oncogenic features of primary pulmonary enteric adenocarcinoma in comparison with invasive adenocarcinoma in resection specimens.
Zhao L, Huang S, Liu J, Zhao J, Li Q, Wang HQ
Medicine96(39)e8153(2017 Sep)

HpSlyD inducing CDX2 and VIL1 expression mediated through TCTP protein may contribute to intestinal metaplasia in the stomach.
Li Q, Zhu Y, Liu J, Yu X, Chen M, Dong N, Gong Y, Yuan Y
Scientific reports7(1)2278(2017 May)

Panel of Villin, Pro-Ex-C, Estrogen Receptor and Progesterone Receptor Expressions Could Help in Differentiation Between Endocervical and Endometrioid Adenocarcinoma.
Elfeky M, Harb O, Gertallah L
Turk patoloji dergisi1(1)29-40(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Villin 1 is released in plasma during acute kidney injury and shows potential as an early marker for proximal tubular injury/necrosis.
Title: Plasmatic Villin 1 Is a Novel In Vivo Marker of Proximal Tubular Cell Injury During Renal Ischemia-Reperfusion.

Villin has a higher positive expression in Primary Pulmonary Enteric Adenocarcinoma cases. Villin proved to be a very sensitive and helpful enteric marker in the differential diagnosis between PEAC and other lung adenocarcinomas.
Title: Clinicopathological, radiographic, and oncogenic features of primary pulmonary enteric adenocarcinoma in comparison with invasive adenocarcinoma in resection specimens.

villin directly interacts with a transcriptional corepressor and ligand of the Slug promoter, ZBRK1.
Title: By moonlighting in the nucleus, villin regulates epithelial plasticity.

Data indicate an association between highly fucosylated glycans and caudal type homeobox 1 (CDX1) and/or villin mRNA expression that both correlate with cell differentiation.
Title: N-glycosylation Profiling of Colorectal Cancer Cell Lines Reveals Association of Fucosylation with Differentiation and Caudal Type Homebox 1 (CDX1)/Villin mRNA Expression.

study demonstrates that G. duodenalis-mediated disruption of villin is, at least, in part dependent on activation of myosin light chain kinase
Title: Giardia duodenalis Surface Cysteine Proteases Induce Cleavage of the Intestinal Epithelial Cytoskeletal Protein Villin via Myosin Light Chain Kinase.

The following VIL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VIL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05971	NM_007127.3 Latest version!	Homo sapiens villin 1 (VIL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
View Old Accession Versions >>
OHu05971	NM_007127.2	Homo sapiens villin 1 (VIL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VIL1 ( NM_007127.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_009058.2 Homo sapiens villin 1 (VIL1), mRNA.

Price & Availability↑

CloneID	OHu05971
Clone ID Related Accession (Same CDS sequence)	NM_007127.2 , NM_007127.3
Accession Version	NM_007127.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	villin-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP329402.1, AK313709.1 and AK223398.1. On Jul 24, 2008 this sequence version replaced NM_007127.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK223398.1, AK313709.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCAAGC TGAGCGCCCA AGTCAAAGGC TCTCTCAACA TCACCACCCC GGGGCTGCAG 
ATATGGAGGA TCGAGGCCAT GCAGATGGTG CCTGTTCCTT CCAGCACCTT TGGAAGCTTC 
TTCGATGGTG ACTGCTACAT CATCCTGGCT ATCCACAAGA CAGCCAGCAG CCTGTCCTAT 
GACATCCACT ACTGGATTGG CCAGGACTCA TCCCTGGATG AGCAGGGGGC AGCTGCCATC 
TACACCACAC AGATGGATGA CTTCCTGAAG GGCCGGGCTG TGCAGCACCG CGAGGTCCAG 
GGCAACGAGA GCGAGGCCTT CCGAGGCTAC TTCAAGCAAG GCCTTGTGAT CCGGAAAGGG 
GGCGTGGCTT CTGGCATGAA GCACGTGGAG ACCAACTCCT ATGACGTCCA GAGGCTGCTG 
CATGTCAAGG GCAAGAGGAA CGTGGTAGCT GGAGAGGTAG AGATGTCCTG GAAGAGTTTC 
AACCGAGGGG ATGTTTTCCT CCTGGACCTT GGGAAGCTTA TCATCCAGTG GAATGGACCG 
GAAAGCACCC GTATGGAGAG ACTCAGGGGC ATGACTCTGG CCAAGGAGAT CCGAGACCAG 
GAGCGGGGAG GGCGCACCTA TGTAGGCGTG GTGGACGGAG AGAATGAATT GGCATCCCCG 
AAGCTGATGG AGGTGATGAA CCACGTGCTG GGCAAGCGCA GGGAGCTGAA GGCGGCCGTG 
CCCGACACGG TGGTGGAGCC GGCACTCAAG GCTGCACTCA AACTGTACCA TGTGTCTGAC 
TCCGAGGGGA ATCTGGTGGT GAGGGAAGTC GCCACACGGC CACTGACACA GGACCTGCTC 
AGTCACGAGG ACTGTTACAT CCTGGACCAG GGGGGCCTGA AGATCTACGT GTGGAAAGGG 
AAGAAAGCCA ATGAGCAGGA GAAGAAGGGA GCCATGAGCC ATGCGCTGAA CTTCATCAAA 
GCCAAGCAGT ACCCACCAAG CACACAGGTG GAGGTGCAGA ATGATGGGGC TGAGTCGGCC 
GTCTTTCAGC AGCTCTTCCA GAAGTGGACA GCGTCCAACC GGACCTCAGG CCTAGGCAAA 
ACCCACACTG TGGGCTCCGT GGCCAAAGTG GAACAGGTGA AGTTCGATGC CACATCCATG 
CATGTCAAGC CTCAGGTGGC TGCCCAGCAG AAGATGGTAG ATGATGGGAG TGGGGAAGTG 
CAGGTGTGGC GCATTGAGAA CCTAGAGCTG GTACCTGTGG ATTCCAAGTG GCTAGGCCAC 
TTCTATGGGG GCGACTGCTA CCTGCTGCTC TACACCTACC TCATCGGCGA GAAGCAGCAT 
TACCTGCTCT ACGTTTGGCA GGGCAGCCAG GCCAGCCAAG ATGAAATTAC AGCATCAGCT 
TATCAAGCCG TCATCCTGGA CCAGAAGTAC AATGGTGAAC CAGTCCAGAT CCGGGTCCCA 
ATGGGCAAGG AGCCACCTCA TCTTATGTCC ATCTTCAAGG GACGCATGGT GGTCTACCAG 
GGAGGCACCT CCCGAACTAA CAACTTGGAG ACCGGGCCCT CCACACGGCT GTTCCAGGTC 
CAGGGAACTG GCGCCAACAA CACCAAGGCC TTTGAGGTCC CAGCGCGGGC CAATTTCCTC 
AATTCCAATG ATGTCTTTGT CCTCAAGACC CAGTCTTGCT GCTATCTATG GTGTGGGAAG 
GGTTGTAGCG GGGACGAGCG GGAGATGGCC AAGATGGTTG CTGACACCAT CTCCCGGACG 
GAGAAGCAAG TGGTGGTGGA AGGGCAGGAG CCAGCCAACT TCTGGATGGC CCTGGGTGGG 
AAGGCCCCCT ATGCCAACAC CAAGAGACTA CAGGAAGAAA ACCTGGTCAT CACCCCCCGG 
CTCTTTGAGT GTTCCAACAA GACTGGGCGC TTCCTGGCCA CAGAGATCCC TGACTTCAAT 
CAGGATGACT TGGAAGAGGA TGATGTGTTC CTACTAGATG TCTGGGACCA GGTCTTCTTC 
TGGATTGGGA AACATGCCAA CGAGGAGGAG AAGAAGGCCG CAGCAACCAC TGCACAGGAA 
TACCTCAAGA CCCATCCCAG CGGGCGTGAC CCTGAGACCC CCATCATTGT GGTGAAGCAG 
GGACACGAGC CCCCCACCTT CACAGGCTGG TTCCTGGCTT GGGATCCCTT CAAGTGGAGT 
AACACCAAAT CCTATGAGGA CCTGAAGGCG GAGCTTGGCA ACTCTAGGGA CTGGAGCCAG 
ATCACTGCTG AGGTCACAAG CCCCAAAGTG GACGTGTTCA ATGCTAACAG CAACCTCAGT 
TCTGGGCCTC TGCCCATCTT CCCCCTGGAG CAGCTAGTGA ACAAGCCTGT AGAGGAGCTC 
CCCGAGGGTG TGGACCCCAG CAGGAAGGAG GAACACCTGT CCATTGAAGA TTTCACTCAG 
GCCTTTGGGA TGACTCCAGC TGCCTTCTCT GCTCTGCCTC GATGGAAGCA ACAAAACCTC 
AAGAAAGAAA AAGGACTATT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009058.2
CDS	66..2549
Translation	MTKLSAQVKGSLNITTPGLQIWRIEAMQMVPVPSSTFGSFFDGDCYIILAIHKTASSLSYDIHYWIGQDSSLDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESEAFRGYFKQGLVIRKGGVASGMKHVETNSYDVQRLLHVKGKRNVVAGEVEMSWKSFNRGDVFLLDLGKLIIQWNGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWTASNRTSGLGKTHTVGSVAKVEQVKFDATSMHVKPQVAAQQKMVDDGSGEVQVWRIENLELVPVDSKWLGHFYGGDCYLLLYTYLIGEKQHYLLYVWQGSQASQDEITASAYQAVILDQKYNGEPVQIRVPMGKEPPHLMSIFKGRMVVYQGGTSRTNNLETGPSTRLFQVQGTGANNTKAFEVPARANFLNSNDVFVLKTQSCCYLWCGKGCSGDEREMAKMVADTISRTEKQVVVEGQEPANFWMALGGKAPYANTKRLQEENLVITPRLFECSNKTGRFLATEIPDFNQDDLEEDDVFLLDVWDQVFFWIGKHANEEEKKAAATTAQEYLKTHPSGRDPETPIIVVKQGHEPPTFTGWFLAWDPFKWSNTKSYEDLKAELGNSRDWSQITAEVTSPKVDVFNANSNLSSGPLPIFPLEQLVNKPVEELPEGVDPSRKEEHLSIEDFTQAFGMTPAAFSALPRWKQQNLKKEKGLF

Target ORF information:

RefSeq Version	NM_007127.2
Organism	Homo sapiens(human)
Definition	Homo sapiens villin 1 (VIL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007127.2

ORF Insert Sequence:

ATGACCAAGC TGAGCGCCCA AGTCAAAGGC TCTCTCAACA TCACCACCCC GGGGCTGCAG 
ATATGGAGGA TCGAGGCCAT GCAGATGGTG CCTGTTCCTT CCAGCACCTT TGGAAGCTTC 
TTCGATGGTG ACTGCTACAT CATCCTGGCT ATCCACAAGA CAGCCAGCAG CCTGTCCTAT 
GACATCCACT ACTGGATTGG CCAGGACTCA TCCCTGGATG AGCAGGGGGC AGCTGCCATC 
TACACCACAC AGATGGATGA CTTCCTGAAG GGCCGGGCTG TGCAGCACCG CGAGGTCCAG 
GGCAACGAGA GCGAGGCCTT CCGAGGCTAC TTCAAGCAAG GCCTTGTGAT CCGGAAAGGG 
GGCGTGGCTT CTGGCATGAA GCACGTGGAG ACCAACTCCT ATGACGTCCA GAGGCTGCTG 
CATGTCAAGG GCAAGAGGAA CGTGGTAGCT GGAGAGGTAG AGATGTCCTG GAAGAGTTTC 
AACCGAGGGG ATGTTTTCCT CCTGGACCTT GGGAAGCTTA TCATCCAGTG GAATGGACCG 
GAAAGCACCC GTATGGAGAG ACTCAGGGGC ATGACTCTGG CCAAGGAGAT CCGAGACCAG 
GAGCGGGGAG GGCGCACCTA TGTAGGCGTG GTGGACGGAG AGAATGAATT GGCATCCCCG 
AAGCTGATGG AGGTGATGAA CCACGTGCTG GGCAAGCGCA GGGAGCTGAA GGCGGCCGTG 
CCCGACACGG TGGTGGAGCC GGCACTCAAG GCTGCACTCA AACTGTACCA TGTGTCTGAC 
TCCGAGGGGA ATCTGGTGGT GAGGGAAGTC GCCACACGGC CACTGACACA GGACCTGCTC 
AGTCACGAGG ACTGTTACAT CCTGGACCAG GGGGGCCTGA AGATCTACGT GTGGAAAGGG 
AAGAAAGCCA ATGAGCAGGA GAAGAAGGGA GCCATGAGCC ATGCGCTGAA CTTCATCAAA 
GCCAAGCAGT ACCCACCAAG CACACAGGTG GAGGTGCAGA ATGATGGGGC TGAGTCGGCC 
GTCTTTCAGC AGCTCTTCCA GAAGTGGACA GCGTCCAACC GGACCTCAGG CCTAGGCAAA 
ACCCACACTG TGGGCTCCGT GGCCAAAGTG GAACAGGTGA AGTTCGATGC CACATCCATG 
CATGTCAAGC CTCAGGTGGC TGCCCAGCAG AAGATGGTAG ATGATGGGAG TGGGGAAGTG 
CAGGTGTGGC GCATTGAGAA CCTAGAGCTG GTACCTGTGG ATTCCAAGTG GCTAGGCCAC 
TTCTATGGGG GCGACTGCTA CCTGCTGCTC TACACCTACC TCATCGGCGA GAAGCAGCAT 
TACCTGCTCT ACGTTTGGCA GGGCAGCCAG GCCAGCCAAG ATGAAATTAC AGCATCAGCT 
TATCAAGCCG TCATCCTGGA CCAGAAGTAC AATGGTGAAC CAGTCCAGAT CCGGGTCCCA 
ATGGGCAAGG AGCCACCTCA TCTTATGTCC ATCTTCAAGG GACGCATGGT GGTCTACCAG 
GGAGGCACCT CCCGAACTAA CAACTTGGAG ACCGGGCCCT CCACACGGCT GTTCCAGGTC 
CAGGGAACTG GCGCCAACAA CACCAAGGCC TTTGAGGTCC CAGCGCGGGC CAATTTCCTC 
AATTCCAATG ATGTCTTTGT CCTCAAGACC CAGTCTTGCT GCTATCTATG GTGTGGGAAG 
GGTTGTAGCG GGGACGAGCG GGAGATGGCC AAGATGGTTG CTGACACCAT CTCCCGGACG 
GAGAAGCAAG TGGTGGTGGA AGGGCAGGAG CCAGCCAACT TCTGGATGGC CCTGGGTGGG 
AAGGCCCCCT ATGCCAACAC CAAGAGACTA CAGGAAGAAA ACCTGGTCAT CACCCCCCGG 
CTCTTTGAGT GTTCCAACAA GACTGGGCGC TTCCTGGCCA CAGAGATCCC TGACTTCAAT 
CAGGATGACT TGGAAGAGGA TGATGTGTTC CTACTAGATG TCTGGGACCA GGTCTTCTTC 
TGGATTGGGA AACATGCCAA CGAGGAGGAG AAGAAGGCCG CAGCAACCAC TGCACAGGAA 
TACCTCAAGA CCCATCCCAG CGGGCGTGAC CCTGAGACCC CCATCATTGT GGTGAAGCAG 
GGACACGAGC CCCCCACCTT CACAGGCTGG TTCCTGGCTT GGGATCCCTT CAAGTGGAGT 
AACACCAAAT CCTATGAGGA CCTGAAGGCG GAGCTTGGCA ACTCTAGGGA CTGGAGCCAG 
ATCACTGCTG AGGTCACAAG CCCCAAAGTG GACGTGTTCA ATGCTAACAG CAACCTCAGT 
TCTGGGCCTC TGCCCATCTT CCCCCTGGAG CAGCTAGTGA ACAAGCCTGT AGAGGAGCTC 
CCCGAGGGTG TGGACCCCAG CAGGAAGGAG GAACACCTGT CCATTGAAGA TTTCACTCAG 
GCCTTTGGGA TGACTCCAGC TGCCTTCTCT GCTCTGCCTC GATGGAAGCA ACAAAACCTC 
AAGAAAGAAA AAGGACTATT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VIL1 ( NM_007127.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_009058.2 Homo sapiens villin 1 (VIL1), mRNA.

Price & Availability↑

CloneID	OHu05971
Clone ID Related Accession (Same CDS sequence)	NM_007127.2 , NM_007127.3
Accession Version	NM_007127.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	villin-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP329402.1, AK313709.1, AK223398.1 and AC073838.6. On May 17, 2019 this sequence version replaced NM_007127.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK313709.1, AK223398.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000248444.10/ ENSP00000248444.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCAAGC TGAGCGCCCA AGTCAAAGGC TCTCTCAACA TCACCACCCC GGGGCTGCAG 
ATATGGAGGA TCGAGGCCAT GCAGATGGTG CCTGTTCCTT CCAGCACCTT TGGAAGCTTC 
TTCGATGGTG ACTGCTACAT CATCCTGGCT ATCCACAAGA CAGCCAGCAG CCTGTCCTAT 
GACATCCACT ACTGGATTGG CCAGGACTCA TCCCTGGATG AGCAGGGGGC AGCTGCCATC 
TACACCACAC AGATGGATGA CTTCCTGAAG GGCCGGGCTG TGCAGCACCG CGAGGTCCAG 
GGCAACGAGA GCGAGGCCTT CCGAGGCTAC TTCAAGCAAG GCCTTGTGAT CCGGAAAGGG 
GGCGTGGCTT CTGGCATGAA GCACGTGGAG ACCAACTCCT ATGACGTCCA GAGGCTGCTG 
CATGTCAAGG GCAAGAGGAA CGTGGTAGCT GGAGAGGTAG AGATGTCCTG GAAGAGTTTC 
AACCGAGGGG ATGTTTTCCT CCTGGACCTT GGGAAGCTTA TCATCCAGTG GAATGGACCG 
GAAAGCACCC GTATGGAGAG ACTCAGGGGC ATGACTCTGG CCAAGGAGAT CCGAGACCAG 
GAGCGGGGAG GGCGCACCTA TGTAGGCGTG GTGGACGGAG AGAATGAATT GGCATCCCCG 
AAGCTGATGG AGGTGATGAA CCACGTGCTG GGCAAGCGCA GGGAGCTGAA GGCGGCCGTG 
CCCGACACGG TGGTGGAGCC GGCACTCAAG GCTGCACTCA AACTGTACCA TGTGTCTGAC 
TCCGAGGGGA ATCTGGTGGT GAGGGAAGTC GCCACACGGC CACTGACACA GGACCTGCTC 
AGTCACGAGG ACTGTTACAT CCTGGACCAG GGGGGCCTGA AGATCTACGT GTGGAAAGGG 
AAGAAAGCCA ATGAGCAGGA GAAGAAGGGA GCCATGAGCC ATGCGCTGAA CTTCATCAAA 
GCCAAGCAGT ACCCACCAAG CACACAGGTG GAGGTGCAGA ATGATGGGGC TGAGTCGGCC 
GTCTTTCAGC AGCTCTTCCA GAAGTGGACA GCGTCCAACC GGACCTCAGG CCTAGGCAAA 
ACCCACACTG TGGGCTCCGT GGCCAAAGTG GAACAGGTGA AGTTCGATGC CACATCCATG 
CATGTCAAGC CTCAGGTGGC TGCCCAGCAG AAGATGGTAG ATGATGGGAG TGGGGAAGTG 
CAGGTGTGGC GCATTGAGAA CCTAGAGCTG GTACCTGTGG ATTCCAAGTG GCTAGGCCAC 
TTCTATGGGG GCGACTGCTA CCTGCTGCTC TACACCTACC TCATCGGCGA GAAGCAGCAT 
TACCTGCTCT ACGTTTGGCA GGGCAGCCAG GCCAGCCAAG ATGAAATTAC AGCATCAGCT 
TATCAAGCCG TCATCCTGGA CCAGAAGTAC AATGGTGAAC CAGTCCAGAT CCGGGTCCCA 
ATGGGCAAGG AGCCACCTCA TCTTATGTCC ATCTTCAAGG GACGCATGGT GGTCTACCAG 
GGAGGCACCT CCCGAACTAA CAACTTGGAG ACCGGGCCCT CCACACGGCT GTTCCAGGTC 
CAGGGAACTG GCGCCAACAA CACCAAGGCC TTTGAGGTCC CAGCGCGGGC CAATTTCCTC 
AATTCCAATG ATGTCTTTGT CCTCAAGACC CAGTCTTGCT GCTATCTATG GTGTGGGAAG 
GGTTGTAGCG GGGACGAGCG GGAGATGGCC AAGATGGTTG CTGACACCAT CTCCCGGACG 
GAGAAGCAAG TGGTGGTGGA AGGGCAGGAG CCAGCCAACT TCTGGATGGC CCTGGGTGGG 
AAGGCCCCCT ATGCCAACAC CAAGAGACTA CAGGAAGAAA ACCTGGTCAT CACCCCCCGG 
CTCTTTGAGT GTTCCAACAA GACTGGGCGC TTCCTGGCCA CAGAGATCCC TGACTTCAAT 
CAGGATGACT TGGAAGAGGA TGATGTGTTC CTACTAGATG TCTGGGACCA GGTCTTCTTC 
TGGATTGGGA AACATGCCAA CGAGGAGGAG AAGAAGGCCG CAGCAACCAC TGCACAGGAA 
TACCTCAAGA CCCATCCCAG CGGGCGTGAC CCTGAGACCC CCATCATTGT GGTGAAGCAG 
GGACACGAGC CCCCCACCTT CACAGGCTGG TTCCTGGCTT GGGATCCCTT CAAGTGGAGT 
AACACCAAAT CCTATGAGGA CCTGAAGGCG GAGCTTGGCA ACTCTAGGGA CTGGAGCCAG 
ATCACTGCTG AGGTCACAAG CCCCAAAGTG GACGTGTTCA ATGCTAACAG CAACCTCAGT 
TCTGGGCCTC TGCCCATCTT CCCCCTGGAG CAGCTAGTGA ACAAGCCTGT AGAGGAGCTC 
CCCGAGGGTG TGGACCCCAG CAGGAAGGAG GAACACCTGT CCATTGAAGA TTTCACTCAG 
GCCTTTGGGA TGACTCCAGC TGCCTTCTCT GCTCTGCCTC GATGGAAGCA ACAAAACCTC 
AAGAAAGAAA AAGGACTATT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009058.2
CDS	58..2541
Translation	MTKLSAQVKGSLNITTPGLQIWRIEAMQMVPVPSSTFGSFFDGDCYIILAIHKTASSLSYDIHYWIGQDSSLDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESEAFRGYFKQGLVIRKGGVASGMKHVETNSYDVQRLLHVKGKRNVVAGEVEMSWKSFNRGDVFLLDLGKLIIQWNGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWTASNRTSGLGKTHTVGSVAKVEQVKFDATSMHVKPQVAAQQKMVDDGSGEVQVWRIENLELVPVDSKWLGHFYGGDCYLLLYTYLIGEKQHYLLYVWQGSQASQDEITASAYQAVILDQKYNGEPVQIRVPMGKEPPHLMSIFKGRMVVYQGGTSRTNNLETGPSTRLFQVQGTGANNTKAFEVPARANFLNSNDVFVLKTQSCCYLWCGKGCSGDEREMAKMVADTISRTEKQVVVEGQEPANFWMALGGKAPYANTKRLQEENLVITPRLFECSNKTGRFLATEIPDFNQDDLEEDDVFLLDVWDQVFFWIGKHANEEEKKAAATTAQEYLKTHPSGRDPETPIIVVKQGHEPPTFTGWFLAWDPFKWSNTKSYEDLKAELGNSRDWSQITAEVTSPKVDVFNANSNLSSGPLPIFPLEQLVNKPVEELPEGVDPSRKEEHLSIEDFTQAFGMTPAAFSALPRWKQQNLKKEKGLF

Target ORF information:

RefSeq Version	NM_007127.3
Organism	Homo sapiens(human)
Definition	Homo sapiens villin 1 (VIL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007127.3

ORF Insert Sequence:

ATGACCAAGC TGAGCGCCCA AGTCAAAGGC TCTCTCAACA TCACCACCCC GGGGCTGCAG 
ATATGGAGGA TCGAGGCCAT GCAGATGGTG CCTGTTCCTT CCAGCACCTT TGGAAGCTTC 
TTCGATGGTG ACTGCTACAT CATCCTGGCT ATCCACAAGA CAGCCAGCAG CCTGTCCTAT 
GACATCCACT ACTGGATTGG CCAGGACTCA TCCCTGGATG AGCAGGGGGC AGCTGCCATC 
TACACCACAC AGATGGATGA CTTCCTGAAG GGCCGGGCTG TGCAGCACCG CGAGGTCCAG 
GGCAACGAGA GCGAGGCCTT CCGAGGCTAC TTCAAGCAAG GCCTTGTGAT CCGGAAAGGG 
GGCGTGGCTT CTGGCATGAA GCACGTGGAG ACCAACTCCT ATGACGTCCA GAGGCTGCTG 
CATGTCAAGG GCAAGAGGAA CGTGGTAGCT GGAGAGGTAG AGATGTCCTG GAAGAGTTTC 
AACCGAGGGG ATGTTTTCCT CCTGGACCTT GGGAAGCTTA TCATCCAGTG GAATGGACCG 
GAAAGCACCC GTATGGAGAG ACTCAGGGGC ATGACTCTGG CCAAGGAGAT CCGAGACCAG 
GAGCGGGGAG GGCGCACCTA TGTAGGCGTG GTGGACGGAG AGAATGAATT GGCATCCCCG 
AAGCTGATGG AGGTGATGAA CCACGTGCTG GGCAAGCGCA GGGAGCTGAA GGCGGCCGTG 
CCCGACACGG TGGTGGAGCC GGCACTCAAG GCTGCACTCA AACTGTACCA TGTGTCTGAC 
TCCGAGGGGA ATCTGGTGGT GAGGGAAGTC GCCACACGGC CACTGACACA GGACCTGCTC 
AGTCACGAGG ACTGTTACAT CCTGGACCAG GGGGGCCTGA AGATCTACGT GTGGAAAGGG 
AAGAAAGCCA ATGAGCAGGA GAAGAAGGGA GCCATGAGCC ATGCGCTGAA CTTCATCAAA 
GCCAAGCAGT ACCCACCAAG CACACAGGTG GAGGTGCAGA ATGATGGGGC TGAGTCGGCC 
GTCTTTCAGC AGCTCTTCCA GAAGTGGACA GCGTCCAACC GGACCTCAGG CCTAGGCAAA 
ACCCACACTG TGGGCTCCGT GGCCAAAGTG GAACAGGTGA AGTTCGATGC CACATCCATG 
CATGTCAAGC CTCAGGTGGC TGCCCAGCAG AAGATGGTAG ATGATGGGAG TGGGGAAGTG 
CAGGTGTGGC GCATTGAGAA CCTAGAGCTG GTACCTGTGG ATTCCAAGTG GCTAGGCCAC 
TTCTATGGGG GCGACTGCTA CCTGCTGCTC TACACCTACC TCATCGGCGA GAAGCAGCAT 
TACCTGCTCT ACGTTTGGCA GGGCAGCCAG GCCAGCCAAG ATGAAATTAC AGCATCAGCT 
TATCAAGCCG TCATCCTGGA CCAGAAGTAC AATGGTGAAC CAGTCCAGAT CCGGGTCCCA 
ATGGGCAAGG AGCCACCTCA TCTTATGTCC ATCTTCAAGG GACGCATGGT GGTCTACCAG 
GGAGGCACCT CCCGAACTAA CAACTTGGAG ACCGGGCCCT CCACACGGCT GTTCCAGGTC 
CAGGGAACTG GCGCCAACAA CACCAAGGCC TTTGAGGTCC CAGCGCGGGC CAATTTCCTC 
AATTCCAATG ATGTCTTTGT CCTCAAGACC CAGTCTTGCT GCTATCTATG GTGTGGGAAG 
GGTTGTAGCG GGGACGAGCG GGAGATGGCC AAGATGGTTG CTGACACCAT CTCCCGGACG 
GAGAAGCAAG TGGTGGTGGA AGGGCAGGAG CCAGCCAACT TCTGGATGGC CCTGGGTGGG 
AAGGCCCCCT ATGCCAACAC CAAGAGACTA CAGGAAGAAA ACCTGGTCAT CACCCCCCGG 
CTCTTTGAGT GTTCCAACAA GACTGGGCGC TTCCTGGCCA CAGAGATCCC TGACTTCAAT 
CAGGATGACT TGGAAGAGGA TGATGTGTTC CTACTAGATG TCTGGGACCA GGTCTTCTTC 
TGGATTGGGA AACATGCCAA CGAGGAGGAG AAGAAGGCCG CAGCAACCAC TGCACAGGAA 
TACCTCAAGA CCCATCCCAG CGGGCGTGAC CCTGAGACCC CCATCATTGT GGTGAAGCAG 
GGACACGAGC CCCCCACCTT CACAGGCTGG TTCCTGGCTT GGGATCCCTT CAAGTGGAGT 
AACACCAAAT CCTATGAGGA CCTGAAGGCG GAGCTTGGCA ACTCTAGGGA CTGGAGCCAG 
ATCACTGCTG AGGTCACAAG CCCCAAAGTG GACGTGTTCA ATGCTAACAG CAACCTCAGT 
TCTGGGCCTC TGCCCATCTT CCCCCTGGAG CAGCTAGTGA ACAAGCCTGT AGAGGAGCTC 
CCCGAGGGTG TGGACCCCAG CAGGAAGGAG GAACACCTGT CCATTGAAGA TTTCACTCAG 
GCCTTTGGGA TGACTCCAGC TGCCTTCTCT GCTCTGCCTC GATGGAAGCA ACAAAACCTC 
AAGAAAGAAA AAGGACTATT TTGA