VARS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VARS1
Entrez Gene ID	7407
Full Name	valyl-tRNA synthetase
Synonyms	G7A,NDMSCA,VARS1,VARS2
General protein information	Preferred Names valyl-tRNA synthetase Names valine--tRNA ligase protein G7a valRS valine tRNA ligase 1, cytoplasmic valyl-tRNA synthetase 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.33
Summary	Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008].
Disorder MIM:	192150
Disorder Html:	Neurodevelopmental disorder with microcephaly, seizures, and cortical atrophy, 617802 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_006295.2	NP_006286.1	valine--tRNA ligase
XM_017011246.1	XP_016866735.1	valine--tRNA ligase isoform X2
NM_006295.3	NP_006286.1	valine--tRNA ligase
XM_017011247.1	XP_016866736.1	valine--tRNA ligase isoform X2
XM_005249362.2	XP_005249419.1	valine--tRNA ligase isoform X1
XM_024446537.1	XP_024302305.1	valine--tRNA ligase isoform X2

Pathways from BioSystems

Homologies

	VARS	XP_001105797.2
Drosophila melanogaster (fruit fly)	Aats-val	NP_001260946.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010420	XP_311528.4
Schizosaccharomyces pombe (fission yeast)	vrs1	NP_595435.1
Homo sapiens (human)	VARS	NP_006286.1
Bos taurus (cattle)	VARS	XP_002697393.2
Mus musculus (house mouse)	Vars	NP_035820.3
Magnaporthe oryzae (rice blast fungus)	MGG_04396	XP_003711020.1
Neurospora crassa	NCU01965	XP_964329.2
Canis lupus familiaris (dog)	VARS	XP_005627219.1
Rattus norvegicus (Norway rat)	Vars	NP_445744.1
Danio rerio (zebrafish)	vars	XP_682807.5
Saccharomyces cerevisiae (baker's yeast)	VAS1	NP_011608.1
Arabidopsis thaliana (thale cress)	TWN2	NP_172913.1
Oryza sativa (rice)	Os03g0694900	NP_001050973.1
Oryza sativa (rice)	Os10g0506200	NP_001176231.1
Xenopus tropicalis (tropical clawed frog)	vars	XP_002939512.2
Caenorhabditis elegans (roundworm)	vars-2	NP_493377.1
Kluyveromyces lactis	KLLA0D14971g	XP_453724.1
Pan troglodytes (chimpanzee)	VARS	XP_518361.2
Eremothecium gossypii	AGOS_AAR034W	NP_982575.1

Gene Ontology
Bibliography

Related articles in PubMed

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Jefferson M, Donaszi-Ivanov A, Pollen S, Dalmay T, Saalbach G, Powell PP
Journal of virology88(18)10340-53(2014 Sep)

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
Talmud PJ, Drenos F, Shah S, Shah T, Palmen J, Verzilli C, Gaunt TR, Pallas J, Lovering R, Li K, Casas JP, Sofat R, Kumari M, Rodriguez S, Johnson T, Newhouse SJ, Dominiczak A, Samani NJ, Caulfield M, Sever P, Stanton A, Shields DC, Padmanabhan S, Melander O, Hastie C, Delles C, Ebrahim S, Marmot MG, Smith GD, Lawlor DA, Munroe PB, Day IN, Kivimaki M, Whittaker J, Humphries SE, Hingorani AD, , ,
American journal of human genetics85(5)628-42(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

Observational study of gene-disease association. (HuGE Navigator)
Title: High-density SNP screening of the major histocompatibility complex in systemic lupus erythematosus demonstrates strong evidence for independent susceptibility regions.

The first three-dimensional structure of the the valyl-tRNA synthetase/elongation factor-1H complex as calculated from electron microscopic images of negatively stained samples of the human ValRS/EF-1H complex was obtained.
Title: Three-dimensional reconstruction of the valyl-tRNA synthetase/elongation factor-1H complex and localization of the delta subunit.

The following VARS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VARS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu61296	XM_024446537.1 Latest version!	Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu61296	XM_017011246.1 Latest version!	Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu61296	XM_017011247.1 Latest version!	Homo sapiens valyl-tRNA synthetase (VARS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu19378	NM_006295.3 Latest version!	Homo sapiens valyl-tRNA synthetase 1 (VARS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$69.30 $99.00
OHu40453	XM_005249362.2 Latest version!	Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu19378	NM_006295.2	Homo sapiens valyl-tRNA synthetase (VARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VARS1 ( XM_017011246.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866735.1 Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu61296
Clone ID Related Accession (Same CDS sequence)	XM_017011246.1 , XM_017011247.1 , XM_024446537.1
Accession Version	XM_017011246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	valine--tRNA ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTTG GCACAGGTGC TGTGAAGATC ACCCCCGCAC ATGACCAAAA TGACTATGAA 
GTTGGGCAGC GGCACGGGCT GGAGGCCATC AGCATCATGG ACTCCCGGGG GGCCCTCATC 
AATGTGCCTC CGCCTTTCCT GGGCCTGCCC AGGTTTGAGG CCAGGAAAGC GGTGCTGGTG 
GCGCTGAAGG AGCGGGGACT GTTCCGTGGC ATTGAGGACA ACCCCATGGT GGTGCCACTT 
TGCAACCGGT CGAAGGACGT GGTAGAGCCT CTGCTGCGGC CGCAGTGGTA CGTTCGCTGC 
GGGGAGATGG CCCAGGCTGC CAGCGCCGCT GTGACTCGGG GTGACCTCCG CATCCTGCCT 
GAGGCCCATC AGCGCACATG GCATGCCTGG ATGGACAACA TCCGGGAGTG GTGCATTTCC 
AGGCAGCTGT GGTGGGGCCA TCGCATCCCA GCCTACTTTG TCACTGTCAG TGACCCAGCG 
GTGCCCCCTG GGGAGGACCC TGATGGGCGG TACTGGGTGA GTGGACGCAA TGAGGCGGAG 
GCCCGGGAGA AGGCAGCCAA GGAGTTCGGA GTGTCCCCTG ACAAGATCAG TCTCCAGCAA 
GATGAGGATG TATTGGATAC CTGGTTCTCC TCTGGCCTCT TCCCCTTATC CATTTTGGGC 
TGGCCCAACC AGTCAGAAGA CCTGAGTGTG TTCTACCCCG GGACACTGCT GGAGACCGGT 
CATGACATCC TCTTCTTCTG GGTGGCCCGG ATGGTCATGC TGGGCCTGAA GCTCACGGGC 
AGGCTGCCCT TTAGAGAGGT CTACCTCCAT GCCATCGTGC GAGATGCTCA CGGCCGGAAG 
ATGAGCAAGT CTCTAGGCAA TGTCATCGAT CCCCTGGACG TCATCTATGG AATCTCCCTG 
CAGGGCCTCC ACAACCAGCT GCTGAACAGC AACCTGGATC CCAGCGAGGT GGAGAAGGCC 
AAAGAAGGGC AGAAAGCTGA CTTCCCAGCG GGGATTCCTG AATGTGGCAC CGATGCTCTC 
CGGTTTGGAT TATGTGCCTA CATGTCCCAG GGTCGTGACA TCAACCTGGA TGTGAACCGG 
ATACTGGGTT ACCGCCACTT CTGCAACAAG CTCTGGAATG CCACCAAGTT TGCCCTTCGT 
GGCCTTGGGA AGGGTTTTGT GCCCTCACCC ACCTCCCAGC CCGGAGGCCA TGAGAGCCTG 
GTGGACCGCT GGATCCGCAG CCGCCTGACA GAGGCTGTGA GGCTCAGCAA TCAAGGCTTC 
CAGGCCTACG ACTTCCCGGC CGTCACCACT GCCCAGTACA GCTTCTGGCT CTATGAGCTC 
TGTGATGTCT ACTTGGAGTG CCTGAAACCT GTACTGAATG GGGTGGACCA GGTGGCAGCT 
GAGTGTGCCC GCCAGACCCT GTACACTTGC CTGGACGTTG GCCTGCGGCT GCTCTCACCC 
TTCATGCCCT TCGTGACGGA GGAGCTGTTC CAGAGGCTGC CCCGGAGGAT GCCGCAAGCT 
CCCCCTAGCC TCTGTGTTAC CCCCTACCCG GAGCCCTCAG AGTGCTCCTG GAAGGACCCC 
GAGGCAGAAG CCGCCCTTGA GCTGGCGCTA AGCATCACGC GAGCCGTGCG CTCCCTGCGG 
GCCGACTACA ACCTCACCCG GATCCGGCCT GACTGTTTCC TGGAAGTGGC GGATGAGGCC 
ACGGGCGCCC TGGCATCGGC GGTGTCGGGC TACGTGCAGG CCCTGGCCAG CGCAGGTGTG 
GTGGCTGTTC TGGCCCTGGG GGCTCCCGCC CCCCAGGGTT GCGCTGTGGC TCTGGCTTCT 
GATCGCTGCT CCATCCACCT GCAGCTTCAG GGGCTGGTGG ACCCTGCACG GGAGCTGGGC 
AAGCTGCAAG CCAAGCGAGT TGAGGCCCAG CGGCAGGCCC AGCGTCTGCG GGAACGCCGT 
GCTGCCTCGG GCTATCCTGT CAAGGTGCCG CTCGAAGTCC AGGAGGCAGA TGAAGCCAAG 
CTCCAACAGA CAGAAGCAGA GCTCAGGAAG GTGGATGAGG CCATCGCCCT ATTCCAGAAG 
ATGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866735.1
CDS	911..2959
Translation	MDFGTGAVKITPAHDQNDYEVGQRHGLEAISIMDSRGALINVPPPFLGLPRFEARKAVLVALKERGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIREWCISRQLWWGHRIPAYFVTVSDPAVPPGEDPDGRYWVSGRNEAEAREKAAKEFGVSPDKISLQQDEDVLDTWFSSGLFPLSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGRLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIYGISLQGLHNQLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYMSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSQPGGHESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRMPQAPPSLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASDRCSIHLQLQGLVDPARELGKLQAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	XM_017011246.1
Organism	Homo sapiens(human)
Definition	Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011246.1

ORF Insert Sequence:

ATGGACTTTG GCACAGGTGC TGTGAAGATC ACCCCCGCAC ATGACCAAAA TGACTATGAA 
GTTGGGCAGC GGCACGGGCT GGAGGCCATC AGCATCATGG ACTCCCGGGG GGCCCTCATC 
AATGTGCCTC CGCCTTTCCT GGGCCTGCCC AGGTTTGAGG CCAGGAAAGC GGTGCTGGTG 
GCGCTGAAGG AGCGGGGACT GTTCCGTGGC ATTGAGGACA ACCCCATGGT GGTGCCACTT 
TGCAACCGGT CGAAGGACGT GGTAGAGCCT CTGCTGCGGC CGCAGTGGTA CGTTCGCTGC 
GGGGAGATGG CCCAGGCTGC CAGCGCCGCT GTGACTCGGG GTGACCTCCG CATCCTGCCT 
GAGGCCCATC AGCGCACATG GCATGCCTGG ATGGACAACA TCCGGGAGTG GTGCATTTCC 
AGGCAGCTGT GGTGGGGCCA TCGCATCCCA GCCTACTTTG TCACTGTCAG TGACCCAGCG 
GTGCCCCCTG GGGAGGACCC TGATGGGCGG TACTGGGTGA GTGGACGCAA TGAGGCGGAG 
GCCCGGGAGA AGGCAGCCAA GGAGTTCGGA GTGTCCCCTG ACAAGATCAG TCTCCAGCAA 
GATGAGGATG TATTGGATAC CTGGTTCTCC TCTGGCCTCT TCCCCTTATC CATTTTGGGC 
TGGCCCAACC AGTCAGAAGA CCTGAGTGTG TTCTACCCCG GGACACTGCT GGAGACCGGT 
CATGACATCC TCTTCTTCTG GGTGGCCCGG ATGGTCATGC TGGGCCTGAA GCTCACGGGC 
AGGCTGCCCT TTAGAGAGGT CTACCTCCAT GCCATCGTGC GAGATGCTCA CGGCCGGAAG 
ATGAGCAAGT CTCTAGGCAA TGTCATCGAT CCCCTGGACG TCATCTATGG AATCTCCCTG 
CAGGGCCTCC ACAACCAGCT GCTGAACAGC AACCTGGATC CCAGCGAGGT GGAGAAGGCC 
AAAGAAGGGC AGAAAGCTGA CTTCCCAGCG GGGATTCCTG AATGTGGCAC CGATGCTCTC 
CGGTTTGGAT TATGTGCCTA CATGTCCCAG GGTCGTGACA TCAACCTGGA TGTGAACCGG 
ATACTGGGTT ACCGCCACTT CTGCAACAAG CTCTGGAATG CCACCAAGTT TGCCCTTCGT 
GGCCTTGGGA AGGGTTTTGT GCCCTCACCC ACCTCCCAGC CCGGAGGCCA TGAGAGCCTG 
GTGGACCGCT GGATCCGCAG CCGCCTGACA GAGGCTGTGA GGCTCAGCAA TCAAGGCTTC 
CAGGCCTACG ACTTCCCGGC CGTCACCACT GCCCAGTACA GCTTCTGGCT CTATGAGCTC 
TGTGATGTCT ACTTGGAGTG CCTGAAACCT GTACTGAATG GGGTGGACCA GGTGGCAGCT 
GAGTGTGCCC GCCAGACCCT GTACACTTGC CTGGACGTTG GCCTGCGGCT GCTCTCACCC 
TTCATGCCCT TCGTGACGGA GGAGCTGTTC CAGAGGCTGC CCCGGAGGAT GCCGCAAGCT 
CCCCCTAGCC TCTGTGTTAC CCCCTACCCG GAGCCCTCAG AGTGCTCCTG GAAGGACCCC 
GAGGCAGAAG CCGCCCTTGA GCTGGCGCTA AGCATCACGC GAGCCGTGCG CTCCCTGCGG 
GCCGACTACA ACCTCACCCG GATCCGGCCT GACTGTTTCC TGGAAGTGGC GGATGAGGCC 
ACGGGCGCCC TGGCATCGGC GGTGTCGGGC TACGTGCAGG CCCTGGCCAG CGCAGGTGTG 
GTGGCTGTTC TGGCCCTGGG GGCTCCCGCC CCCCAGGGTT GCGCTGTGGC TCTGGCTTCT 
GATCGCTGCT CCATCCACCT GCAGCTTCAG GGGCTGGTGG ACCCTGCACG GGAGCTGGGC 
AAGCTGCAAG CCAAGCGAGT TGAGGCCCAG CGGCAGGCCC AGCGTCTGCG GGAACGCCGT 
GCTGCCTCGG GCTATCCTGT CAAGGTGCCG CTCGAAGTCC AGGAGGCAGA TGAAGCCAAG 
CTCCAACAGA CAGAAGCAGA GCTCAGGAAG GTGGATGAGG CCATCGCCCT ATTCCAGAAG 
ATGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS1 ( XM_017011247.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866736.1 Homo sapiens valyl-tRNA synthetase (VARS), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu61296
Clone ID Related Accession (Same CDS sequence)	XM_017011246.1 , XM_017011247.1 , XM_024446537.1
Accession Version	XM_017011247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	valine--tRNA ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTTG GCACAGGTGC TGTGAAGATC ACCCCCGCAC ATGACCAAAA TGACTATGAA 
GTTGGGCAGC GGCACGGGCT GGAGGCCATC AGCATCATGG ACTCCCGGGG GGCCCTCATC 
AATGTGCCTC CGCCTTTCCT GGGCCTGCCC AGGTTTGAGG CCAGGAAAGC GGTGCTGGTG 
GCGCTGAAGG AGCGGGGACT GTTCCGTGGC ATTGAGGACA ACCCCATGGT GGTGCCACTT 
TGCAACCGGT CGAAGGACGT GGTAGAGCCT CTGCTGCGGC CGCAGTGGTA CGTTCGCTGC 
GGGGAGATGG CCCAGGCTGC CAGCGCCGCT GTGACTCGGG GTGACCTCCG CATCCTGCCT 
GAGGCCCATC AGCGCACATG GCATGCCTGG ATGGACAACA TCCGGGAGTG GTGCATTTCC 
AGGCAGCTGT GGTGGGGCCA TCGCATCCCA GCCTACTTTG TCACTGTCAG TGACCCAGCG 
GTGCCCCCTG GGGAGGACCC TGATGGGCGG TACTGGGTGA GTGGACGCAA TGAGGCGGAG 
GCCCGGGAGA AGGCAGCCAA GGAGTTCGGA GTGTCCCCTG ACAAGATCAG TCTCCAGCAA 
GATGAGGATG TATTGGATAC CTGGTTCTCC TCTGGCCTCT TCCCCTTATC CATTTTGGGC 
TGGCCCAACC AGTCAGAAGA CCTGAGTGTG TTCTACCCCG GGACACTGCT GGAGACCGGT 
CATGACATCC TCTTCTTCTG GGTGGCCCGG ATGGTCATGC TGGGCCTGAA GCTCACGGGC 
AGGCTGCCCT TTAGAGAGGT CTACCTCCAT GCCATCGTGC GAGATGCTCA CGGCCGGAAG 
ATGAGCAAGT CTCTAGGCAA TGTCATCGAT CCCCTGGACG TCATCTATGG AATCTCCCTG 
CAGGGCCTCC ACAACCAGCT GCTGAACAGC AACCTGGATC CCAGCGAGGT GGAGAAGGCC 
AAAGAAGGGC AGAAAGCTGA CTTCCCAGCG GGGATTCCTG AATGTGGCAC CGATGCTCTC 
CGGTTTGGAT TATGTGCCTA CATGTCCCAG GGTCGTGACA TCAACCTGGA TGTGAACCGG 
ATACTGGGTT ACCGCCACTT CTGCAACAAG CTCTGGAATG CCACCAAGTT TGCCCTTCGT 
GGCCTTGGGA AGGGTTTTGT GCCCTCACCC ACCTCCCAGC CCGGAGGCCA TGAGAGCCTG 
GTGGACCGCT GGATCCGCAG CCGCCTGACA GAGGCTGTGA GGCTCAGCAA TCAAGGCTTC 
CAGGCCTACG ACTTCCCGGC CGTCACCACT GCCCAGTACA GCTTCTGGCT CTATGAGCTC 
TGTGATGTCT ACTTGGAGTG CCTGAAACCT GTACTGAATG GGGTGGACCA GGTGGCAGCT 
GAGTGTGCCC GCCAGACCCT GTACACTTGC CTGGACGTTG GCCTGCGGCT GCTCTCACCC 
TTCATGCCCT TCGTGACGGA GGAGCTGTTC CAGAGGCTGC CCCGGAGGAT GCCGCAAGCT 
CCCCCTAGCC TCTGTGTTAC CCCCTACCCG GAGCCCTCAG AGTGCTCCTG GAAGGACCCC 
GAGGCAGAAG CCGCCCTTGA GCTGGCGCTA AGCATCACGC GAGCCGTGCG CTCCCTGCGG 
GCCGACTACA ACCTCACCCG GATCCGGCCT GACTGTTTCC TGGAAGTGGC GGATGAGGCC 
ACGGGCGCCC TGGCATCGGC GGTGTCGGGC TACGTGCAGG CCCTGGCCAG CGCAGGTGTG 
GTGGCTGTTC TGGCCCTGGG GGCTCCCGCC CCCCAGGGTT GCGCTGTGGC TCTGGCTTCT 
GATCGCTGCT CCATCCACCT GCAGCTTCAG GGGCTGGTGG ACCCTGCACG GGAGCTGGGC 
AAGCTGCAAG CCAAGCGAGT TGAGGCCCAG CGGCAGGCCC AGCGTCTGCG GGAACGCCGT 
GCTGCCTCGG GCTATCCTGT CAAGGTGCCG CTCGAAGTCC AGGAGGCAGA TGAAGCCAAG 
CTCCAACAGA CAGAAGCAGA GCTCAGGAAG GTGGATGAGG CCATCGCCCT ATTCCAGAAG 
ATGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866736.1
CDS	914..2962
Translation	MDFGTGAVKITPAHDQNDYEVGQRHGLEAISIMDSRGALINVPPPFLGLPRFEARKAVLVALKERGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIREWCISRQLWWGHRIPAYFVTVSDPAVPPGEDPDGRYWVSGRNEAEAREKAAKEFGVSPDKISLQQDEDVLDTWFSSGLFPLSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGRLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIYGISLQGLHNQLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYMSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSQPGGHESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRMPQAPPSLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASDRCSIHLQLQGLVDPARELGKLQAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	XM_017011247.1
Organism	Homo sapiens(human)
Definition	Homo sapiens valyl-tRNA synthetase (VARS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011247.1

ORF Insert Sequence:

ATGGACTTTG GCACAGGTGC TGTGAAGATC ACCCCCGCAC ATGACCAAAA TGACTATGAA 
GTTGGGCAGC GGCACGGGCT GGAGGCCATC AGCATCATGG ACTCCCGGGG GGCCCTCATC 
AATGTGCCTC CGCCTTTCCT GGGCCTGCCC AGGTTTGAGG CCAGGAAAGC GGTGCTGGTG 
GCGCTGAAGG AGCGGGGACT GTTCCGTGGC ATTGAGGACA ACCCCATGGT GGTGCCACTT 
TGCAACCGGT CGAAGGACGT GGTAGAGCCT CTGCTGCGGC CGCAGTGGTA CGTTCGCTGC 
GGGGAGATGG CCCAGGCTGC CAGCGCCGCT GTGACTCGGG GTGACCTCCG CATCCTGCCT 
GAGGCCCATC AGCGCACATG GCATGCCTGG ATGGACAACA TCCGGGAGTG GTGCATTTCC 
AGGCAGCTGT GGTGGGGCCA TCGCATCCCA GCCTACTTTG TCACTGTCAG TGACCCAGCG 
GTGCCCCCTG GGGAGGACCC TGATGGGCGG TACTGGGTGA GTGGACGCAA TGAGGCGGAG 
GCCCGGGAGA AGGCAGCCAA GGAGTTCGGA GTGTCCCCTG ACAAGATCAG TCTCCAGCAA 
GATGAGGATG TATTGGATAC CTGGTTCTCC TCTGGCCTCT TCCCCTTATC CATTTTGGGC 
TGGCCCAACC AGTCAGAAGA CCTGAGTGTG TTCTACCCCG GGACACTGCT GGAGACCGGT 
CATGACATCC TCTTCTTCTG GGTGGCCCGG ATGGTCATGC TGGGCCTGAA GCTCACGGGC 
AGGCTGCCCT TTAGAGAGGT CTACCTCCAT GCCATCGTGC GAGATGCTCA CGGCCGGAAG 
ATGAGCAAGT CTCTAGGCAA TGTCATCGAT CCCCTGGACG TCATCTATGG AATCTCCCTG 
CAGGGCCTCC ACAACCAGCT GCTGAACAGC AACCTGGATC CCAGCGAGGT GGAGAAGGCC 
AAAGAAGGGC AGAAAGCTGA CTTCCCAGCG GGGATTCCTG AATGTGGCAC CGATGCTCTC 
CGGTTTGGAT TATGTGCCTA CATGTCCCAG GGTCGTGACA TCAACCTGGA TGTGAACCGG 
ATACTGGGTT ACCGCCACTT CTGCAACAAG CTCTGGAATG CCACCAAGTT TGCCCTTCGT 
GGCCTTGGGA AGGGTTTTGT GCCCTCACCC ACCTCCCAGC CCGGAGGCCA TGAGAGCCTG 
GTGGACCGCT GGATCCGCAG CCGCCTGACA GAGGCTGTGA GGCTCAGCAA TCAAGGCTTC 
CAGGCCTACG ACTTCCCGGC CGTCACCACT GCCCAGTACA GCTTCTGGCT CTATGAGCTC 
TGTGATGTCT ACTTGGAGTG CCTGAAACCT GTACTGAATG GGGTGGACCA GGTGGCAGCT 
GAGTGTGCCC GCCAGACCCT GTACACTTGC CTGGACGTTG GCCTGCGGCT GCTCTCACCC 
TTCATGCCCT TCGTGACGGA GGAGCTGTTC CAGAGGCTGC CCCGGAGGAT GCCGCAAGCT 
CCCCCTAGCC TCTGTGTTAC CCCCTACCCG GAGCCCTCAG AGTGCTCCTG GAAGGACCCC 
GAGGCAGAAG CCGCCCTTGA GCTGGCGCTA AGCATCACGC GAGCCGTGCG CTCCCTGCGG 
GCCGACTACA ACCTCACCCG GATCCGGCCT GACTGTTTCC TGGAAGTGGC GGATGAGGCC 
ACGGGCGCCC TGGCATCGGC GGTGTCGGGC TACGTGCAGG CCCTGGCCAG CGCAGGTGTG 
GTGGCTGTTC TGGCCCTGGG GGCTCCCGCC CCCCAGGGTT GCGCTGTGGC TCTGGCTTCT 
GATCGCTGCT CCATCCACCT GCAGCTTCAG GGGCTGGTGG ACCCTGCACG GGAGCTGGGC 
AAGCTGCAAG CCAAGCGAGT TGAGGCCCAG CGGCAGGCCC AGCGTCTGCG GGAACGCCGT 
GCTGCCTCGG GCTATCCTGT CAAGGTGCCG CTCGAAGTCC AGGAGGCAGA TGAAGCCAAG 
CTCCAACAGA CAGAAGCAGA GCTCAGGAAG GTGGATGAGG CCATCGCCCT ATTCCAGAAG 
ATGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS1 ( XM_024446537.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302305.1 Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X4, mRNA.

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CloneID	OHu61296
Clone ID Related Accession (Same CDS sequence)	XM_017011246.1 , XM_017011247.1 , XM_024446537.1
Accession Version	XM_024446537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	valine--tRNA ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTTG GCACAGGTGC TGTGAAGATC ACCCCCGCAC ATGACCAAAA TGACTATGAA 
GTTGGGCAGC GGCACGGGCT GGAGGCCATC AGCATCATGG ACTCCCGGGG GGCCCTCATC 
AATGTGCCTC CGCCTTTCCT GGGCCTGCCC AGGTTTGAGG CCAGGAAAGC GGTGCTGGTG 
GCGCTGAAGG AGCGGGGACT GTTCCGTGGC ATTGAGGACA ACCCCATGGT GGTGCCACTT 
TGCAACCGGT CGAAGGACGT GGTAGAGCCT CTGCTGCGGC CGCAGTGGTA CGTTCGCTGC 
GGGGAGATGG CCCAGGCTGC CAGCGCCGCT GTGACTCGGG GTGACCTCCG CATCCTGCCT 
GAGGCCCATC AGCGCACATG GCATGCCTGG ATGGACAACA TCCGGGAGTG GTGCATTTCC 
AGGCAGCTGT GGTGGGGCCA TCGCATCCCA GCCTACTTTG TCACTGTCAG TGACCCAGCG 
GTGCCCCCTG GGGAGGACCC TGATGGGCGG TACTGGGTGA GTGGACGCAA TGAGGCGGAG 
GCCCGGGAGA AGGCAGCCAA GGAGTTCGGA GTGTCCCCTG ACAAGATCAG TCTCCAGCAA 
GATGAGGATG TATTGGATAC CTGGTTCTCC TCTGGCCTCT TCCCCTTATC CATTTTGGGC 
TGGCCCAACC AGTCAGAAGA CCTGAGTGTG TTCTACCCCG GGACACTGCT GGAGACCGGT 
CATGACATCC TCTTCTTCTG GGTGGCCCGG ATGGTCATGC TGGGCCTGAA GCTCACGGGC 
AGGCTGCCCT TTAGAGAGGT CTACCTCCAT GCCATCGTGC GAGATGCTCA CGGCCGGAAG 
ATGAGCAAGT CTCTAGGCAA TGTCATCGAT CCCCTGGACG TCATCTATGG AATCTCCCTG 
CAGGGCCTCC ACAACCAGCT GCTGAACAGC AACCTGGATC CCAGCGAGGT GGAGAAGGCC 
AAAGAAGGGC AGAAAGCTGA CTTCCCAGCG GGGATTCCTG AATGTGGCAC CGATGCTCTC 
CGGTTTGGAT TATGTGCCTA CATGTCCCAG GGTCGTGACA TCAACCTGGA TGTGAACCGG 
ATACTGGGTT ACCGCCACTT CTGCAACAAG CTCTGGAATG CCACCAAGTT TGCCCTTCGT 
GGCCTTGGGA AGGGTTTTGT GCCCTCACCC ACCTCCCAGC CCGGAGGCCA TGAGAGCCTG 
GTGGACCGCT GGATCCGCAG CCGCCTGACA GAGGCTGTGA GGCTCAGCAA TCAAGGCTTC 
CAGGCCTACG ACTTCCCGGC CGTCACCACT GCCCAGTACA GCTTCTGGCT CTATGAGCTC 
TGTGATGTCT ACTTGGAGTG CCTGAAACCT GTACTGAATG GGGTGGACCA GGTGGCAGCT 
GAGTGTGCCC GCCAGACCCT GTACACTTGC CTGGACGTTG GCCTGCGGCT GCTCTCACCC 
TTCATGCCCT TCGTGACGGA GGAGCTGTTC CAGAGGCTGC CCCGGAGGAT GCCGCAAGCT 
CCCCCTAGCC TCTGTGTTAC CCCCTACCCG GAGCCCTCAG AGTGCTCCTG GAAGGACCCC 
GAGGCAGAAG CCGCCCTTGA GCTGGCGCTA AGCATCACGC GAGCCGTGCG CTCCCTGCGG 
GCCGACTACA ACCTCACCCG GATCCGGCCT GACTGTTTCC TGGAAGTGGC GGATGAGGCC 
ACGGGCGCCC TGGCATCGGC GGTGTCGGGC TACGTGCAGG CCCTGGCCAG CGCAGGTGTG 
GTGGCTGTTC TGGCCCTGGG GGCTCCCGCC CCCCAGGGTT GCGCTGTGGC TCTGGCTTCT 
GATCGCTGCT CCATCCACCT GCAGCTTCAG GGGCTGGTGG ACCCTGCACG GGAGCTGGGC 
AAGCTGCAAG CCAAGCGAGT TGAGGCCCAG CGGCAGGCCC AGCGTCTGCG GGAACGCCGT 
GCTGCCTCGG GCTATCCTGT CAAGGTGCCG CTCGAAGTCC AGGAGGCAGA TGAAGCCAAG 
CTCCAACAGA CAGAAGCAGA GCTCAGGAAG GTGGATGAGG CCATCGCCCT ATTCCAGAAG 
ATGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302305.1
CDS	1025..3073
Translation	MDFGTGAVKITPAHDQNDYEVGQRHGLEAISIMDSRGALINVPPPFLGLPRFEARKAVLVALKERGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIREWCISRQLWWGHRIPAYFVTVSDPAVPPGEDPDGRYWVSGRNEAEAREKAAKEFGVSPDKISLQQDEDVLDTWFSSGLFPLSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGRLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIYGISLQGLHNQLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYMSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSQPGGHESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRMPQAPPSLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASDRCSIHLQLQGLVDPARELGKLQAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	XM_024446537.1
Organism	Homo sapiens(human)
Definition	Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446537.1

ORF Insert Sequence:

ATGGACTTTG GCACAGGTGC TGTGAAGATC ACCCCCGCAC ATGACCAAAA TGACTATGAA 
GTTGGGCAGC GGCACGGGCT GGAGGCCATC AGCATCATGG ACTCCCGGGG GGCCCTCATC 
AATGTGCCTC CGCCTTTCCT GGGCCTGCCC AGGTTTGAGG CCAGGAAAGC GGTGCTGGTG 
GCGCTGAAGG AGCGGGGACT GTTCCGTGGC ATTGAGGACA ACCCCATGGT GGTGCCACTT 
TGCAACCGGT CGAAGGACGT GGTAGAGCCT CTGCTGCGGC CGCAGTGGTA CGTTCGCTGC 
GGGGAGATGG CCCAGGCTGC CAGCGCCGCT GTGACTCGGG GTGACCTCCG CATCCTGCCT 
GAGGCCCATC AGCGCACATG GCATGCCTGG ATGGACAACA TCCGGGAGTG GTGCATTTCC 
AGGCAGCTGT GGTGGGGCCA TCGCATCCCA GCCTACTTTG TCACTGTCAG TGACCCAGCG 
GTGCCCCCTG GGGAGGACCC TGATGGGCGG TACTGGGTGA GTGGACGCAA TGAGGCGGAG 
GCCCGGGAGA AGGCAGCCAA GGAGTTCGGA GTGTCCCCTG ACAAGATCAG TCTCCAGCAA 
GATGAGGATG TATTGGATAC CTGGTTCTCC TCTGGCCTCT TCCCCTTATC CATTTTGGGC 
TGGCCCAACC AGTCAGAAGA CCTGAGTGTG TTCTACCCCG GGACACTGCT GGAGACCGGT 
CATGACATCC TCTTCTTCTG GGTGGCCCGG ATGGTCATGC TGGGCCTGAA GCTCACGGGC 
AGGCTGCCCT TTAGAGAGGT CTACCTCCAT GCCATCGTGC GAGATGCTCA CGGCCGGAAG 
ATGAGCAAGT CTCTAGGCAA TGTCATCGAT CCCCTGGACG TCATCTATGG AATCTCCCTG 
CAGGGCCTCC ACAACCAGCT GCTGAACAGC AACCTGGATC CCAGCGAGGT GGAGAAGGCC 
AAAGAAGGGC AGAAAGCTGA CTTCCCAGCG GGGATTCCTG AATGTGGCAC CGATGCTCTC 
CGGTTTGGAT TATGTGCCTA CATGTCCCAG GGTCGTGACA TCAACCTGGA TGTGAACCGG 
ATACTGGGTT ACCGCCACTT CTGCAACAAG CTCTGGAATG CCACCAAGTT TGCCCTTCGT 
GGCCTTGGGA AGGGTTTTGT GCCCTCACCC ACCTCCCAGC CCGGAGGCCA TGAGAGCCTG 
GTGGACCGCT GGATCCGCAG CCGCCTGACA GAGGCTGTGA GGCTCAGCAA TCAAGGCTTC 
CAGGCCTACG ACTTCCCGGC CGTCACCACT GCCCAGTACA GCTTCTGGCT CTATGAGCTC 
TGTGATGTCT ACTTGGAGTG CCTGAAACCT GTACTGAATG GGGTGGACCA GGTGGCAGCT 
GAGTGTGCCC GCCAGACCCT GTACACTTGC CTGGACGTTG GCCTGCGGCT GCTCTCACCC 
TTCATGCCCT TCGTGACGGA GGAGCTGTTC CAGAGGCTGC CCCGGAGGAT GCCGCAAGCT 
CCCCCTAGCC TCTGTGTTAC CCCCTACCCG GAGCCCTCAG AGTGCTCCTG GAAGGACCCC 
GAGGCAGAAG CCGCCCTTGA GCTGGCGCTA AGCATCACGC GAGCCGTGCG CTCCCTGCGG 
GCCGACTACA ACCTCACCCG GATCCGGCCT GACTGTTTCC TGGAAGTGGC GGATGAGGCC 
ACGGGCGCCC TGGCATCGGC GGTGTCGGGC TACGTGCAGG CCCTGGCCAG CGCAGGTGTG 
GTGGCTGTTC TGGCCCTGGG GGCTCCCGCC CCCCAGGGTT GCGCTGTGGC TCTGGCTTCT 
GATCGCTGCT CCATCCACCT GCAGCTTCAG GGGCTGGTGG ACCCTGCACG GGAGCTGGGC 
AAGCTGCAAG CCAAGCGAGT TGAGGCCCAG CGGCAGGCCC AGCGTCTGCG GGAACGCCGT 
GCTGCCTCGG GCTATCCTGT CAAGGTGCCG CTCGAAGTCC AGGAGGCAGA TGAAGCCAAG 
CTCCAACAGA CAGAAGCAGA GCTCAGGAAG GTGGATGAGG CCATCGCCCT ATTCCAGAAG 
ATGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS1 ( NM_006295.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_006286.1 Homo sapiens valyl-tRNA synthetase (VARS), mRNA.

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CloneID	OHu19378
Clone ID Related Accession (Same CDS sequence)	NM_006295.2 , NM_006295.3
Accession Version	NM_006295.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	valine--tRNA ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CX781162.1, CX167000.1, BC012808.2 and M98326.1. This sequence is a reference standard in the RefSeqGene project. On May 4, 2006 this sequence version replaced NM_006295.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X59303.1, BC012808.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCACCC TCTACGTCTC CCCTCACCCA GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
GCCGCTCGCT ATGGGGAGGC TGGGGAGGGT CCCGGATGGG GAGGAGCCCA CCCCCGCATC 
TGTCTCCAGC CACCCCCGAC TAGCAGGACT CCCTTTCCCC CACCCCGCCT GCCGGCCCTG 
GAGCAGGGGC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGGCCCA GCTGCTGTGG 
CCAGCAGGCC TGGGGGGCCC AGGGGGCAGC CGGGCGGCTG TCCTTGTCCA ACAGTGGGTC 
AGTTACGCCG ACACGGAGTT AATACCAGCT GCCTGTGGAG CAACGCTGCC GGCCCTGGGA 
CTCCGAAGCT CGGCCCAGGA CCCCCAGGCT GTGCTGGGGG CCCTGGGCAG GGCCCTGAGC 
CCCTTGGAGG AGTGGCTTCG GCTGCACACC TACTTGGCCG GGGAGGCCCC CACTCTGGCT 
GACCTGGCGG CTGTCACAGC CTTGCTGCTG CCTTTCCGAT ACGTCCTAGA CCCACCTGCC 
CGCCGGATCT GGAATAATGT GACTCGCTGG TTTGTCACGT GTGTCCGGCA GCCAGAATTC 
CGAGCCGTGC TAGGAGAAGT GGTTCTATAC TCAGGAGCCA GGCCTCTCTC TCATCAGCCA 
GGCCCCGAGG CTCCTGCCCT CCCAAAGACA GCTGCTCAGC TCAAGAAAGA GGCAAAGAAA 
CGGGAGAAGC TAGAGAAATT CCAACAGAAG CAGAAGATCC AACAGCAGCA GCCACCTCCA 
GGGGAGAAGA AACCAAAACC AGAGAAGAGG GAGAAACGGG ATCCTGGGGT CATTACCTAT 
GACCTCCCAA CCCCACCCGG GGAAAAGAAA GATGTCAGTG GCCCCATGCC CGACTCCTAC 
AGCCCTCGGT ATGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCAGCAGGG CTTCTTCAAG 
CCAGAGTATG GGCGTCCTAA TGTGTCAGCA GCAAATCCCC GAGGTGTCTT CATGATGTGC 
ATCCCACCCC CCAATGTGAC AGGCTCCCTG CACCTGGGCC ATGCACTCAC CAACGCCATC 
CAGGACTCCC TGACTCGATG GCACCGCATG CGTGGGGAGA CCACCCTGTG GAACCCTGGC 
TGTGACCATG CAGGTATTGC CACCCAGGTG GTGGTGGAGA AGAAGCTATG GCGTGAGCAG 
GGACTGAGCC GGCACCAGCT GGGCCGCGAG GCCTTTCTAC AGGAAGTCTG GAAGTGGAAG 
GAGGAGAAAG GTGACCGGAT TTACCACCAG TTGAAGAAGC TTGGCAGCTC CTTGGACTGG 
GATCGAGCCT GTTTCACCAT GGACCCTAAA CTCTCAGCAG CTGTGACAGA GGCCTTTGTC 
CGGCTTCACG AGGAAGGCAT CATCTATCGC AGTACCCGCC TTGTTAACTG GTCCTGCACC 
CTCAACTCCG CCATCTCTGA CATTGAGGTG GATAAGAAGG AGCTGACAGG TCGCACCCTG 
CTCTCCGTGC CTGGCTACAA GGAGAAGGTG GAGTTCGGGG TCCTCGTGTC CTTTGCCTAT 
AAGGTCCAAG GCTCAGATAG CGACGAGGAG GTGGTGGTGG CAACAACTCG GATCGAGACA 
ATGCTGGGAG ATGTGGCTGT AGCTGTGCAC CCCAAAGATA CCAGATACCA GCACCTGAAG 
GGGAAGAACG TGATCCACCC ATTCCTGTCT CGGAGCCTTC CCATTGTCTT CGATGAATTT 
GTGGACATGG ACTTTGGCAC AGGTGCTGTG AAGATCACCC CCGCACATGA CCAAAATGAC 
TATGAAGTTG GGCAGCGGCA CGGGCTGGAG GCCATCAGCA TCATGGACTC CCGGGGGGCC 
CTCATCAATG TGCCTCCGCC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG GAAAGCGGTG 
CTGGTGGCGC TGAAGGAGCG GGGACTGTTC CGTGGCATTG AGGACAACCC CATGGTGGTG 
CCACTTTGCA ACCGGTCGAA GGACGTGGTA GAGCCTCTGC TGCGGCCGCA GTGGTACGTT 
CGCTGCGGGG AGATGGCCCA GGCTGCCAGC GCCGCTGTGA CTCGGGGTGA CCTCCGCATC 
CTGCCTGAGG CCCATCAGCG CACATGGCAT GCCTGGATGG ACAACATCCG GGAGTGGTGC 
ATTTCCAGGC AGCTGTGGTG GGGCCATCGC ATCCCAGCCT ACTTTGTCAC TGTCAGTGAC 
CCAGCGGTGC CCCCTGGGGA GGACCCTGAT GGGCGGTACT GGGTGAGTGG ACGCAATGAG 
GCGGAGGCCC GGGAGAAGGC AGCCAAGGAG TTCGGAGTGT CCCCTGACAA GATCAGTCTC 
CAGCAAGATG AGGATGTATT GGATACCTGG TTCTCCTCTG GCCTCTTCCC CTTATCCATT 
TTGGGCTGGC CCAACCAGTC AGAAGACCTG AGTGTGTTCT ACCCCGGGAC ACTGCTGGAG 
ACCGGTCATG ACATCCTCTT CTTCTGGGTG GCCCGGATGG TCATGCTGGG CCTGAAGCTC 
ACGGGCAGGC TGCCCTTTAG AGAGGTCTAC CTCCATGCCA TCGTGCGAGA TGCTCACGGC 
CGGAAGATGA GCAAGTCTCT AGGCAATGTC ATCGATCCCC TGGACGTCAT CTATGGAATC 
TCCCTGCAGG GCCTCCACAA CCAGCTGCTG AACAGCAACC TGGATCCCAG CGAGGTGGAG 
AAGGCCAAAG AAGGGCAGAA AGCTGACTTC CCAGCGGGGA TTCCTGAATG TGGCACCGAT 
GCTCTCCGGT TTGGATTATG TGCCTACATG TCCCAGGGTC GTGACATCAA CCTGGATGTG 
AACCGGATAC TGGGTTACCG CCACTTCTGC AACAAGCTCT GGAATGCCAC CAAGTTTGCC 
CTTCGTGGCC TTGGGAAGGG TTTTGTGCCC TCACCCACCT CCCAGCCCGG AGGCCATGAG 
AGCCTGGTGG ACCGCTGGAT CCGCAGCCGC CTGACAGAGG CTGTGAGGCT CAGCAATCAA 
GGCTTCCAGG CCTACGACTT CCCGGCCGTC ACCACTGCCC AGTACAGCTT CTGGCTCTAT 
GAGCTCTGTG ATGTCTACTT GGAGTGCCTG AAACCTGTAC TGAATGGGGT GGACCAGGTG 
GCAGCTGAGT GTGCCCGCCA GACCCTGTAC ACTTGCCTGG ACGTTGGCCT GCGGCTGCTC 
TCACCCTTCA TGCCCTTCGT GACGGAGGAG CTGTTCCAGA GGCTGCCCCG GAGGATGCCG 
CAAGCTCCCC CTAGCCTCTG TGTTACCCCC TACCCGGAGC CCTCAGAGTG CTCCTGGAAG 
GACCCCGAGG CAGAAGCCGC CCTTGAGCTG GCGCTAAGCA TCACGCGAGC CGTGCGCTCC 
CTGCGGGCCG ACTACAACCT CACCCGGATC CGGCCTGACT GTTTCCTGGA AGTGGCGGAT 
GAGGCCACGG GCGCCCTGGC ATCGGCGGTG TCGGGCTACG TGCAGGCCCT GGCCAGCGCA 
GGTGTGGTGG CTGTTCTGGC CCTGGGGGCT CCCGCCCCCC AGGGTTGCGC TGTGGCTCTG 
GCTTCTGATC GCTGCTCCAT CCACCTGCAG CTTCAGGGGC TGGTGGACCC TGCACGGGAG 
CTGGGCAAGC TGCAAGCCAA GCGAGTTGAG GCCCAGCGGC AGGCCCAGCG TCTGCGGGAA 
CGCCGTGCTG CCTCGGGCTA TCCTGTCAAG GTGCCGCTCG AAGTCCAGGA GGCAGATGAA 
GCCAAGCTCC AACAGACAGA AGCAGAGCTC AGGAAGGTGG ATGAGGCCAT CGCCCTATTC 
CAGAAGATGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006286.1
CDS	424..4218
Translation	MSTLYVSPHPDAFPSLRALIAARYGEAGEGPGWGGAHPRICLQPPPTSRTPFPPPRLPALEQGPGGLWVWGATAVAQLLWPAGLGGPGGSRAAVLVQQWVSYADTELIPAACGATLPALGLRSSAQDPQAVLGALGRALSPLEEWLRLHTYLAGEAPTLADLAAVTALLLPFRYVLDPPARRIWNNVTRWFVTCVRQPEFRAVLGEVVLYSGARPLSHQPGPEAPALPKTAAQLKKEAKKREKLEKFQQKQKIQQQQPPPGEKKPKPEKREKRDPGVITYDLPTPPGEKKDVSGPMPDSYSPRYVEAAWYPWWEQQGFFKPEYGRPNVSAANPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLSRHQLGREAFLQEVWKWKEEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSAAVTEAFVRLHEEGIIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDTRYQHLKGKNVIHPFLSRSLPIVFDEFVDMDFGTGAVKITPAHDQNDYEVGQRHGLEAISIMDSRGALINVPPPFLGLPRFEARKAVLVALKERGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIREWCISRQLWWGHRIPAYFVTVSDPAVPPGEDPDGRYWVSGRNEAEAREKAAKEFGVSPDKISLQQDEDVLDTWFSSGLFPLSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGRLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIYGISLQGLHNQLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYMSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSQPGGHESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRMPQAPPSLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASDRCSIHLQLQGLVDPARELGKLQAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	NM_006295.2
Organism	Homo sapiens(human)
Definition	Homo sapiens valyl-tRNA synthetase (VARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006295.2

ORF Insert Sequence:

ATGTCCACCC TCTACGTCTC CCCTCACCCA GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
GCCGCTCGCT ATGGGGAGGC TGGGGAGGGT CCCGGATGGG GAGGAGCCCA CCCCCGCATC 
TGTCTCCAGC CACCCCCGAC TAGCAGGACT CCCTTTCCCC CACCCCGCCT GCCGGCCCTG 
GAGCAGGGGC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGGCCCA GCTGCTGTGG 
CCAGCAGGCC TGGGGGGCCC AGGGGGCAGC CGGGCGGCTG TCCTTGTCCA ACAGTGGGTC 
AGTTACGCCG ACACGGAGTT AATACCAGCT GCCTGTGGAG CAACGCTGCC GGCCCTGGGA 
CTCCGAAGCT CGGCCCAGGA CCCCCAGGCT GTGCTGGGGG CCCTGGGCAG GGCCCTGAGC 
CCCTTGGAGG AGTGGCTTCG GCTGCACACC TACTTGGCCG GGGAGGCCCC CACTCTGGCT 
GACCTGGCGG CTGTCACAGC CTTGCTGCTG CCTTTCCGAT ACGTCCTAGA CCCACCTGCC 
CGCCGGATCT GGAATAATGT GACTCGCTGG TTTGTCACGT GTGTCCGGCA GCCAGAATTC 
CGAGCCGTGC TAGGAGAAGT GGTTCTATAC TCAGGAGCCA GGCCTCTCTC TCATCAGCCA 
GGCCCCGAGG CTCCTGCCCT CCCAAAGACA GCTGCTCAGC TCAAGAAAGA GGCAAAGAAA 
CGGGAGAAGC TAGAGAAATT CCAACAGAAG CAGAAGATCC AACAGCAGCA GCCACCTCCA 
GGGGAGAAGA AACCAAAACC AGAGAAGAGG GAGAAACGGG ATCCTGGGGT CATTACCTAT 
GACCTCCCAA CCCCACCCGG GGAAAAGAAA GATGTCAGTG GCCCCATGCC CGACTCCTAC 
AGCCCTCGGT ATGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCAGCAGGG CTTCTTCAAG 
CCAGAGTATG GGCGTCCTAA TGTGTCAGCA GCAAATCCCC GAGGTGTCTT CATGATGTGC 
ATCCCACCCC CCAATGTGAC AGGCTCCCTG CACCTGGGCC ATGCACTCAC CAACGCCATC 
CAGGACTCCC TGACTCGATG GCACCGCATG CGTGGGGAGA CCACCCTGTG GAACCCTGGC 
TGTGACCATG CAGGTATTGC CACCCAGGTG GTGGTGGAGA AGAAGCTATG GCGTGAGCAG 
GGACTGAGCC GGCACCAGCT GGGCCGCGAG GCCTTTCTAC AGGAAGTCTG GAAGTGGAAG 
GAGGAGAAAG GTGACCGGAT TTACCACCAG TTGAAGAAGC TTGGCAGCTC CTTGGACTGG 
GATCGAGCCT GTTTCACCAT GGACCCTAAA CTCTCAGCAG CTGTGACAGA GGCCTTTGTC 
CGGCTTCACG AGGAAGGCAT CATCTATCGC AGTACCCGCC TTGTTAACTG GTCCTGCACC 
CTCAACTCCG CCATCTCTGA CATTGAGGTG GATAAGAAGG AGCTGACAGG TCGCACCCTG 
CTCTCCGTGC CTGGCTACAA GGAGAAGGTG GAGTTCGGGG TCCTCGTGTC CTTTGCCTAT 
AAGGTCCAAG GCTCAGATAG CGACGAGGAG GTGGTGGTGG CAACAACTCG GATCGAGACA 
ATGCTGGGAG ATGTGGCTGT AGCTGTGCAC CCCAAAGATA CCAGATACCA GCACCTGAAG 
GGGAAGAACG TGATCCACCC ATTCCTGTCT CGGAGCCTTC CCATTGTCTT CGATGAATTT 
GTGGACATGG ACTTTGGCAC AGGTGCTGTG AAGATCACCC CCGCACATGA CCAAAATGAC 
TATGAAGTTG GGCAGCGGCA CGGGCTGGAG GCCATCAGCA TCATGGACTC CCGGGGGGCC 
CTCATCAATG TGCCTCCGCC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG GAAAGCGGTG 
CTGGTGGCGC TGAAGGAGCG GGGACTGTTC CGTGGCATTG AGGACAACCC CATGGTGGTG 
CCACTTTGCA ACCGGTCGAA GGACGTGGTA GAGCCTCTGC TGCGGCCGCA GTGGTACGTT 
CGCTGCGGGG AGATGGCCCA GGCTGCCAGC GCCGCTGTGA CTCGGGGTGA CCTCCGCATC 
CTGCCTGAGG CCCATCAGCG CACATGGCAT GCCTGGATGG ACAACATCCG GGAGTGGTGC 
ATTTCCAGGC AGCTGTGGTG GGGCCATCGC ATCCCAGCCT ACTTTGTCAC TGTCAGTGAC 
CCAGCGGTGC CCCCTGGGGA GGACCCTGAT GGGCGGTACT GGGTGAGTGG ACGCAATGAG 
GCGGAGGCCC GGGAGAAGGC AGCCAAGGAG TTCGGAGTGT CCCCTGACAA GATCAGTCTC 
CAGCAAGATG AGGATGTATT GGATACCTGG TTCTCCTCTG GCCTCTTCCC CTTATCCATT 
TTGGGCTGGC CCAACCAGTC AGAAGACCTG AGTGTGTTCT ACCCCGGGAC ACTGCTGGAG 
ACCGGTCATG ACATCCTCTT CTTCTGGGTG GCCCGGATGG TCATGCTGGG CCTGAAGCTC 
ACGGGCAGGC TGCCCTTTAG AGAGGTCTAC CTCCATGCCA TCGTGCGAGA TGCTCACGGC 
CGGAAGATGA GCAAGTCTCT AGGCAATGTC ATCGATCCCC TGGACGTCAT CTATGGAATC 
TCCCTGCAGG GCCTCCACAA CCAGCTGCTG AACAGCAACC TGGATCCCAG CGAGGTGGAG 
AAGGCCAAAG AAGGGCAGAA AGCTGACTTC CCAGCGGGGA TTCCTGAATG TGGCACCGAT 
GCTCTCCGGT TTGGATTATG TGCCTACATG TCCCAGGGTC GTGACATCAA CCTGGATGTG 
AACCGGATAC TGGGTTACCG CCACTTCTGC AACAAGCTCT GGAATGCCAC CAAGTTTGCC 
CTTCGTGGCC TTGGGAAGGG TTTTGTGCCC TCACCCACCT CCCAGCCCGG AGGCCATGAG 
AGCCTGGTGG ACCGCTGGAT CCGCAGCCGC CTGACAGAGG CTGTGAGGCT CAGCAATCAA 
GGCTTCCAGG CCTACGACTT CCCGGCCGTC ACCACTGCCC AGTACAGCTT CTGGCTCTAT 
GAGCTCTGTG ATGTCTACTT GGAGTGCCTG AAACCTGTAC TGAATGGGGT GGACCAGGTG 
GCAGCTGAGT GTGCCCGCCA GACCCTGTAC ACTTGCCTGG ACGTTGGCCT GCGGCTGCTC 
TCACCCTTCA TGCCCTTCGT GACGGAGGAG CTGTTCCAGA GGCTGCCCCG GAGGATGCCG 
CAAGCTCCCC CTAGCCTCTG TGTTACCCCC TACCCGGAGC CCTCAGAGTG CTCCTGGAAG 
GACCCCGAGG CAGAAGCCGC CCTTGAGCTG GCGCTAAGCA TCACGCGAGC CGTGCGCTCC 
CTGCGGGCCG ACTACAACCT CACCCGGATC CGGCCTGACT GTTTCCTGGA AGTGGCGGAT 
GAGGCCACGG GCGCCCTGGC ATCGGCGGTG TCGGGCTACG TGCAGGCCCT GGCCAGCGCA 
GGTGTGGTGG CTGTTCTGGC CCTGGGGGCT CCCGCCCCCC AGGGTTGCGC TGTGGCTCTG 
GCTTCTGATC GCTGCTCCAT CCACCTGCAG CTTCAGGGGC TGGTGGACCC TGCACGGGAG 
CTGGGCAAGC TGCAAGCCAA GCGAGTTGAG GCCCAGCGGC AGGCCCAGCG TCTGCGGGAA 
CGCCGTGCTG CCTCGGGCTA TCCTGTCAAG GTGCCGCTCG AAGTCCAGGA GGCAGATGAA 
GCCAAGCTCC AACAGACAGA AGCAGAGCTC AGGAAGGTGG ATGAGGCCAT CGCCCTATTC 
CAGAAGATGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS1 ( NM_006295.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006286.1 Homo sapiens valyl-tRNA synthetase 1 (VARS1), mRNA.

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CloneID	OHu19378
Clone ID Related Accession (Same CDS sequence)	NM_006295.2 , NM_006295.3
Accession Version	NM_006295.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Homo sapiens(human)
Product	valine--tRNA ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CX781162.1, CX167000.1, BC012808.2 and M98326.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_006295.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X59303.1, BC012808.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology MANE Ensembl match :: ENST00000375663.8/ ENSP00000364815.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCACCC TCTACGTCTC CCCTCACCCA GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
GCCGCTCGCT ATGGGGAGGC TGGGGAGGGT CCCGGATGGG GAGGAGCCCA CCCCCGCATC 
TGTCTCCAGC CACCCCCGAC TAGCAGGACT CCCTTTCCCC CACCCCGCCT GCCGGCCCTG 
GAGCAGGGGC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGGCCCA GCTGCTGTGG 
CCAGCAGGCC TGGGGGGCCC AGGGGGCAGC CGGGCGGCTG TCCTTGTCCA ACAGTGGGTC 
AGTTACGCCG ACACGGAGTT AATACCAGCT GCCTGTGGAG CAACGCTGCC GGCCCTGGGA 
CTCCGAAGCT CGGCCCAGGA CCCCCAGGCT GTGCTGGGGG CCCTGGGCAG GGCCCTGAGC 
CCCTTGGAGG AGTGGCTTCG GCTGCACACC TACTTGGCCG GGGAGGCCCC CACTCTGGCT 
GACCTGGCGG CTGTCACAGC CTTGCTGCTG CCTTTCCGAT ACGTCCTAGA CCCACCTGCC 
CGCCGGATCT GGAATAATGT GACTCGCTGG TTTGTCACGT GTGTCCGGCA GCCAGAATTC 
CGAGCCGTGC TAGGAGAAGT GGTTCTATAC TCAGGAGCCA GGCCTCTCTC TCATCAGCCA 
GGCCCCGAGG CTCCTGCCCT CCCAAAGACA GCTGCTCAGC TCAAGAAAGA GGCAAAGAAA 
CGGGAGAAGC TAGAGAAATT CCAACAGAAG CAGAAGATCC AACAGCAGCA GCCACCTCCA 
GGGGAGAAGA AACCAAAACC AGAGAAGAGG GAGAAACGGG ATCCTGGGGT CATTACCTAT 
GACCTCCCAA CCCCACCCGG GGAAAAGAAA GATGTCAGTG GCCCCATGCC CGACTCCTAC 
AGCCCTCGGT ATGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCAGCAGGG CTTCTTCAAG 
CCAGAGTATG GGCGTCCTAA TGTGTCAGCA GCAAATCCCC GAGGTGTCTT CATGATGTGC 
ATCCCACCCC CCAATGTGAC AGGCTCCCTG CACCTGGGCC ATGCACTCAC CAACGCCATC 
CAGGACTCCC TGACTCGATG GCACCGCATG CGTGGGGAGA CCACCCTGTG GAACCCTGGC 
TGTGACCATG CAGGTATTGC CACCCAGGTG GTGGTGGAGA AGAAGCTATG GCGTGAGCAG 
GGACTGAGCC GGCACCAGCT GGGCCGCGAG GCCTTTCTAC AGGAAGTCTG GAAGTGGAAG 
GAGGAGAAAG GTGACCGGAT TTACCACCAG TTGAAGAAGC TTGGCAGCTC CTTGGACTGG 
GATCGAGCCT GTTTCACCAT GGACCCTAAA CTCTCAGCAG CTGTGACAGA GGCCTTTGTC 
CGGCTTCACG AGGAAGGCAT CATCTATCGC AGTACCCGCC TTGTTAACTG GTCCTGCACC 
CTCAACTCCG CCATCTCTGA CATTGAGGTG GATAAGAAGG AGCTGACAGG TCGCACCCTG 
CTCTCCGTGC CTGGCTACAA GGAGAAGGTG GAGTTCGGGG TCCTCGTGTC CTTTGCCTAT 
AAGGTCCAAG GCTCAGATAG CGACGAGGAG GTGGTGGTGG CAACAACTCG GATCGAGACA 
ATGCTGGGAG ATGTGGCTGT AGCTGTGCAC CCCAAAGATA CCAGATACCA GCACCTGAAG 
GGGAAGAACG TGATCCACCC ATTCCTGTCT CGGAGCCTTC CCATTGTCTT CGATGAATTT 
GTGGACATGG ACTTTGGCAC AGGTGCTGTG AAGATCACCC CCGCACATGA CCAAAATGAC 
TATGAAGTTG GGCAGCGGCA CGGGCTGGAG GCCATCAGCA TCATGGACTC CCGGGGGGCC 
CTCATCAATG TGCCTCCGCC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG GAAAGCGGTG 
CTGGTGGCGC TGAAGGAGCG GGGACTGTTC CGTGGCATTG AGGACAACCC CATGGTGGTG 
CCACTTTGCA ACCGGTCGAA GGACGTGGTA GAGCCTCTGC TGCGGCCGCA GTGGTACGTT 
CGCTGCGGGG AGATGGCCCA GGCTGCCAGC GCCGCTGTGA CTCGGGGTGA CCTCCGCATC 
CTGCCTGAGG CCCATCAGCG CACATGGCAT GCCTGGATGG ACAACATCCG GGAGTGGTGC 
ATTTCCAGGC AGCTGTGGTG GGGCCATCGC ATCCCAGCCT ACTTTGTCAC TGTCAGTGAC 
CCAGCGGTGC CCCCTGGGGA GGACCCTGAT GGGCGGTACT GGGTGAGTGG ACGCAATGAG 
GCGGAGGCCC GGGAGAAGGC AGCCAAGGAG TTCGGAGTGT CCCCTGACAA GATCAGTCTC 
CAGCAAGATG AGGATGTATT GGATACCTGG TTCTCCTCTG GCCTCTTCCC CTTATCCATT 
TTGGGCTGGC CCAACCAGTC AGAAGACCTG AGTGTGTTCT ACCCCGGGAC ACTGCTGGAG 
ACCGGTCATG ACATCCTCTT CTTCTGGGTG GCCCGGATGG TCATGCTGGG CCTGAAGCTC 
ACGGGCAGGC TGCCCTTTAG AGAGGTCTAC CTCCATGCCA TCGTGCGAGA TGCTCACGGC 
CGGAAGATGA GCAAGTCTCT AGGCAATGTC ATCGATCCCC TGGACGTCAT CTATGGAATC 
TCCCTGCAGG GCCTCCACAA CCAGCTGCTG AACAGCAACC TGGATCCCAG CGAGGTGGAG 
AAGGCCAAAG AAGGGCAGAA AGCTGACTTC CCAGCGGGGA TTCCTGAATG TGGCACCGAT 
GCTCTCCGGT TTGGATTATG TGCCTACATG TCCCAGGGTC GTGACATCAA CCTGGATGTG 
AACCGGATAC TGGGTTACCG CCACTTCTGC AACAAGCTCT GGAATGCCAC CAAGTTTGCC 
CTTCGTGGCC TTGGGAAGGG TTTTGTGCCC TCACCCACCT CCCAGCCCGG AGGCCATGAG 
AGCCTGGTGG ACCGCTGGAT CCGCAGCCGC CTGACAGAGG CTGTGAGGCT CAGCAATCAA 
GGCTTCCAGG CCTACGACTT CCCGGCCGTC ACCACTGCCC AGTACAGCTT CTGGCTCTAT 
GAGCTCTGTG ATGTCTACTT GGAGTGCCTG AAACCTGTAC TGAATGGGGT GGACCAGGTG 
GCAGCTGAGT GTGCCCGCCA GACCCTGTAC ACTTGCCTGG ACGTTGGCCT GCGGCTGCTC 
TCACCCTTCA TGCCCTTCGT GACGGAGGAG CTGTTCCAGA GGCTGCCCCG GAGGATGCCG 
CAAGCTCCCC CTAGCCTCTG TGTTACCCCC TACCCGGAGC CCTCAGAGTG CTCCTGGAAG 
GACCCCGAGG CAGAAGCCGC CCTTGAGCTG GCGCTAAGCA TCACGCGAGC CGTGCGCTCC 
CTGCGGGCCG ACTACAACCT CACCCGGATC CGGCCTGACT GTTTCCTGGA AGTGGCGGAT 
GAGGCCACGG GCGCCCTGGC ATCGGCGGTG TCGGGCTACG TGCAGGCCCT GGCCAGCGCA 
GGTGTGGTGG CTGTTCTGGC CCTGGGGGCT CCCGCCCCCC AGGGTTGCGC TGTGGCTCTG 
GCTTCTGATC GCTGCTCCAT CCACCTGCAG CTTCAGGGGC TGGTGGACCC TGCACGGGAG 
CTGGGCAAGC TGCAAGCCAA GCGAGTTGAG GCCCAGCGGC AGGCCCAGCG TCTGCGGGAA 
CGCCGTGCTG CCTCGGGCTA TCCTGTCAAG GTGCCGCTCG AAGTCCAGGA GGCAGATGAA 
GCCAAGCTCC AACAGACAGA AGCAGAGCTC AGGAAGGTGG ATGAGGCCAT CGCCCTATTC 
CAGAAGATGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006286.1
CDS	241..4035
Translation	MSTLYVSPHPDAFPSLRALIAARYGEAGEGPGWGGAHPRICLQPPPTSRTPFPPPRLPALEQGPGGLWVWGATAVAQLLWPAGLGGPGGSRAAVLVQQWVSYADTELIPAACGATLPALGLRSSAQDPQAVLGALGRALSPLEEWLRLHTYLAGEAPTLADLAAVTALLLPFRYVLDPPARRIWNNVTRWFVTCVRQPEFRAVLGEVVLYSGARPLSHQPGPEAPALPKTAAQLKKEAKKREKLEKFQQKQKIQQQQPPPGEKKPKPEKREKRDPGVITYDLPTPPGEKKDVSGPMPDSYSPRYVEAAWYPWWEQQGFFKPEYGRPNVSAANPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLSRHQLGREAFLQEVWKWKEEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSAAVTEAFVRLHEEGIIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDTRYQHLKGKNVIHPFLSRSLPIVFDEFVDMDFGTGAVKITPAHDQNDYEVGQRHGLEAISIMDSRGALINVPPPFLGLPRFEARKAVLVALKERGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIREWCISRQLWWGHRIPAYFVTVSDPAVPPGEDPDGRYWVSGRNEAEAREKAAKEFGVSPDKISLQQDEDVLDTWFSSGLFPLSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGRLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIYGISLQGLHNQLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYMSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSQPGGHESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRMPQAPPSLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASDRCSIHLQLQGLVDPARELGKLQAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	NM_006295.3
Organism	Homo sapiens(human)
Definition	Homo sapiens valyl-tRNA synthetase 1 (VARS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006295.3

ORF Insert Sequence:

ATGTCCACCC TCTACGTCTC CCCTCACCCA GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
GCCGCTCGCT ATGGGGAGGC TGGGGAGGGT CCCGGATGGG GAGGAGCCCA CCCCCGCATC 
TGTCTCCAGC CACCCCCGAC TAGCAGGACT CCCTTTCCCC CACCCCGCCT GCCGGCCCTG 
GAGCAGGGGC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGGCCCA GCTGCTGTGG 
CCAGCAGGCC TGGGGGGCCC AGGGGGCAGC CGGGCGGCTG TCCTTGTCCA ACAGTGGGTC 
AGTTACGCCG ACACGGAGTT AATACCAGCT GCCTGTGGAG CAACGCTGCC GGCCCTGGGA 
CTCCGAAGCT CGGCCCAGGA CCCCCAGGCT GTGCTGGGGG CCCTGGGCAG GGCCCTGAGC 
CCCTTGGAGG AGTGGCTTCG GCTGCACACC TACTTGGCCG GGGAGGCCCC CACTCTGGCT 
GACCTGGCGG CTGTCACAGC CTTGCTGCTG CCTTTCCGAT ACGTCCTAGA CCCACCTGCC 
CGCCGGATCT GGAATAATGT GACTCGCTGG TTTGTCACGT GTGTCCGGCA GCCAGAATTC 
CGAGCCGTGC TAGGAGAAGT GGTTCTATAC TCAGGAGCCA GGCCTCTCTC TCATCAGCCA 
GGCCCCGAGG CTCCTGCCCT CCCAAAGACA GCTGCTCAGC TCAAGAAAGA GGCAAAGAAA 
CGGGAGAAGC TAGAGAAATT CCAACAGAAG CAGAAGATCC AACAGCAGCA GCCACCTCCA 
GGGGAGAAGA AACCAAAACC AGAGAAGAGG GAGAAACGGG ATCCTGGGGT CATTACCTAT 
GACCTCCCAA CCCCACCCGG GGAAAAGAAA GATGTCAGTG GCCCCATGCC CGACTCCTAC 
AGCCCTCGGT ATGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCAGCAGGG CTTCTTCAAG 
CCAGAGTATG GGCGTCCTAA TGTGTCAGCA GCAAATCCCC GAGGTGTCTT CATGATGTGC 
ATCCCACCCC CCAATGTGAC AGGCTCCCTG CACCTGGGCC ATGCACTCAC CAACGCCATC 
CAGGACTCCC TGACTCGATG GCACCGCATG CGTGGGGAGA CCACCCTGTG GAACCCTGGC 
TGTGACCATG CAGGTATTGC CACCCAGGTG GTGGTGGAGA AGAAGCTATG GCGTGAGCAG 
GGACTGAGCC GGCACCAGCT GGGCCGCGAG GCCTTTCTAC AGGAAGTCTG GAAGTGGAAG 
GAGGAGAAAG GTGACCGGAT TTACCACCAG TTGAAGAAGC TTGGCAGCTC CTTGGACTGG 
GATCGAGCCT GTTTCACCAT GGACCCTAAA CTCTCAGCAG CTGTGACAGA GGCCTTTGTC 
CGGCTTCACG AGGAAGGCAT CATCTATCGC AGTACCCGCC TTGTTAACTG GTCCTGCACC 
CTCAACTCCG CCATCTCTGA CATTGAGGTG GATAAGAAGG AGCTGACAGG TCGCACCCTG 
CTCTCCGTGC CTGGCTACAA GGAGAAGGTG GAGTTCGGGG TCCTCGTGTC CTTTGCCTAT 
AAGGTCCAAG GCTCAGATAG CGACGAGGAG GTGGTGGTGG CAACAACTCG GATCGAGACA 
ATGCTGGGAG ATGTGGCTGT AGCTGTGCAC CCCAAAGATA CCAGATACCA GCACCTGAAG 
GGGAAGAACG TGATCCACCC ATTCCTGTCT CGGAGCCTTC CCATTGTCTT CGATGAATTT 
GTGGACATGG ACTTTGGCAC AGGTGCTGTG AAGATCACCC CCGCACATGA CCAAAATGAC 
TATGAAGTTG GGCAGCGGCA CGGGCTGGAG GCCATCAGCA TCATGGACTC CCGGGGGGCC 
CTCATCAATG TGCCTCCGCC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG GAAAGCGGTG 
CTGGTGGCGC TGAAGGAGCG GGGACTGTTC CGTGGCATTG AGGACAACCC CATGGTGGTG 
CCACTTTGCA ACCGGTCGAA GGACGTGGTA GAGCCTCTGC TGCGGCCGCA GTGGTACGTT 
CGCTGCGGGG AGATGGCCCA GGCTGCCAGC GCCGCTGTGA CTCGGGGTGA CCTCCGCATC 
CTGCCTGAGG CCCATCAGCG CACATGGCAT GCCTGGATGG ACAACATCCG GGAGTGGTGC 
ATTTCCAGGC AGCTGTGGTG GGGCCATCGC ATCCCAGCCT ACTTTGTCAC TGTCAGTGAC 
CCAGCGGTGC CCCCTGGGGA GGACCCTGAT GGGCGGTACT GGGTGAGTGG ACGCAATGAG 
GCGGAGGCCC GGGAGAAGGC AGCCAAGGAG TTCGGAGTGT CCCCTGACAA GATCAGTCTC 
CAGCAAGATG AGGATGTATT GGATACCTGG TTCTCCTCTG GCCTCTTCCC CTTATCCATT 
TTGGGCTGGC CCAACCAGTC AGAAGACCTG AGTGTGTTCT ACCCCGGGAC ACTGCTGGAG 
ACCGGTCATG ACATCCTCTT CTTCTGGGTG GCCCGGATGG TCATGCTGGG CCTGAAGCTC 
ACGGGCAGGC TGCCCTTTAG AGAGGTCTAC CTCCATGCCA TCGTGCGAGA TGCTCACGGC 
CGGAAGATGA GCAAGTCTCT AGGCAATGTC ATCGATCCCC TGGACGTCAT CTATGGAATC 
TCCCTGCAGG GCCTCCACAA CCAGCTGCTG AACAGCAACC TGGATCCCAG CGAGGTGGAG 
AAGGCCAAAG AAGGGCAGAA AGCTGACTTC CCAGCGGGGA TTCCTGAATG TGGCACCGAT 
GCTCTCCGGT TTGGATTATG TGCCTACATG TCCCAGGGTC GTGACATCAA CCTGGATGTG 
AACCGGATAC TGGGTTACCG CCACTTCTGC AACAAGCTCT GGAATGCCAC CAAGTTTGCC 
CTTCGTGGCC TTGGGAAGGG TTTTGTGCCC TCACCCACCT CCCAGCCCGG AGGCCATGAG 
AGCCTGGTGG ACCGCTGGAT CCGCAGCCGC CTGACAGAGG CTGTGAGGCT CAGCAATCAA 
GGCTTCCAGG CCTACGACTT CCCGGCCGTC ACCACTGCCC AGTACAGCTT CTGGCTCTAT 
GAGCTCTGTG ATGTCTACTT GGAGTGCCTG AAACCTGTAC TGAATGGGGT GGACCAGGTG 
GCAGCTGAGT GTGCCCGCCA GACCCTGTAC ACTTGCCTGG ACGTTGGCCT GCGGCTGCTC 
TCACCCTTCA TGCCCTTCGT GACGGAGGAG CTGTTCCAGA GGCTGCCCCG GAGGATGCCG 
CAAGCTCCCC CTAGCCTCTG TGTTACCCCC TACCCGGAGC CCTCAGAGTG CTCCTGGAAG 
GACCCCGAGG CAGAAGCCGC CCTTGAGCTG GCGCTAAGCA TCACGCGAGC CGTGCGCTCC 
CTGCGGGCCG ACTACAACCT CACCCGGATC CGGCCTGACT GTTTCCTGGA AGTGGCGGAT 
GAGGCCACGG GCGCCCTGGC ATCGGCGGTG TCGGGCTACG TGCAGGCCCT GGCCAGCGCA 
GGTGTGGTGG CTGTTCTGGC CCTGGGGGCT CCCGCCCCCC AGGGTTGCGC TGTGGCTCTG 
GCTTCTGATC GCTGCTCCAT CCACCTGCAG CTTCAGGGGC TGGTGGACCC TGCACGGGAG 
CTGGGCAAGC TGCAAGCCAA GCGAGTTGAG GCCCAGCGGC AGGCCCAGCG TCTGCGGGAA 
CGCCGTGCTG CCTCGGGCTA TCCTGTCAAG GTGCCGCTCG AAGTCCAGGA GGCAGATGAA 
GCCAAGCTCC AACAGACAGA AGCAGAGCTC AGGAAGGTGG ATGAGGCCAT CGCCCTATTC 
CAGAAGATGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS1 ( XM_005249362.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249419.1 Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X1, mRNA.

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CloneID	OHu40453
Clone ID Related Accession (Same CDS sequence)	XM_005249362.2
Accession Version	XM_005249362.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	valine--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005249362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACCC TCTACGTCTC CCCTCACCCA GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
GCCGCTCGCT ATGGGGAGGC TGGGGAGGGT CCCGGATGGG GAGGAGCCCA CCCCCGCATC 
TGTCTCCAGC CACCCCCGAC TAGCAGGACT CCCTTTCCCC CACCCCGCCT GCCGGCCCTG 
GAGCAGGGGC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGGCCCA GCTGCTGTGG 
CCAGCAGGCC TGGGGGGCCC AGGGGGCAGC CGGGCGGCTG TCCTTGTCCA ACAGTGGGTC 
AGTTACGCCG ACACGGAGTT AATACCAGCT GCCTGTGGAG CAACGCTGCC GGCCCTGGGA 
CTCCGAAGCT CGGCCCAGGA CCCCCAGGCT GTGCTGGGGG CCCTGGGCAG GGCCCTGAGC 
CCCTTGGAGG AGTGGCTTCG GCTGCACACC TACTTGGCCG GGGAGGCCCC CACTCTGGCT 
GACCTGGCGG CTGTCACAGC CTTGCTGCTG CCTTTCCGAT ACGTCCTAGA CCCACCTGCC 
CGCCGGATCT GGAATAATGT GACTCGCTGG TTTGTCACGT GTGTCCGGCA GCCAGAATTC 
CGAGCCGTGC TAGGAGAAGT GGTTCTATAC TCAGGAGCCA GGCCTCTCTC TCATCAGCCA 
GGCCCCGAGG CTCCTGCCCT CCCAAAGACA GCTGCTCAGC TCAAGAAAGA GGCAAAGAAA 
CGGGAGAAGC TAGAGAAATT CCAACAGAAG CAGAAGATCC AACAGCAGCA GCCACCTCCA 
GGGGAGCAGA AGAAACCAAA ACCAGAGAAG AGGGAGAAAC GGGATCCTGG GGTCATTACC 
TATGACCTCC CAACCCCACC CGGGGAAAAG AAAGATGTCA GTGGCCCCAT GCCCGACTCC 
TACAGCCCTC GGTATGTGGA GGCTGCCTGG TACCCTTGGT GGGAGCAGCA GGGCTTCTTC 
AAGCCAGAGT ATGGGCGTCC TAATGTGTCA GCAGCAAATC CCCGAGGTGT CTTCATGATG 
TGCATCCCAC CCCCCAATGT GACAGGCTCC CTGCACCTGG GCCATGCACT CACCAACGCC 
ATCCAGGACT CCCTGACTCG ATGGCACCGC ATGCGTGGGG AGACCACCCT GTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCCACCCAG GTGGTGGTGG AGAAGAAGCT ATGGCGTGAG 
CAGGGACTGA GCCGGCACCA GCTGGGCCGC GAGGCCTTTC TACAGGAAGT CTGGAAGTGG 
AAGGAGGAGA AAGGTGACCG GATTTACCAC CAGTTGAAGA AGCTTGGCAG CTCCTTGGAC 
TGGGATCGAG CCTGTTTCAC CATGGACCCT AAACTCTCAG CAGCTGTGAC AGAGGCCTTT 
GTCCGGCTTC ACGAGGAAGG CATCATCTAT CGCAGTACCC GCCTTGTTAA CTGGTCCTGC 
ACCCTCAACT CCGCCATCTC TGACATTGAG GTGGATAAGA AGGAGCTGAC AGGTCGCACC 
CTGCTCTCCG TGCCTGGCTA CAAGGAGAAG GTGGAGTTCG GGGTCCTCGT GTCCTTTGCC 
TATAAGGTCC AAGGCTCAGA TAGCGACGAG GAGGTGGTGG TGGCAACAAC TCGGATCGAG 
ACAATGCTGG GAGATGTGGC TGTAGCTGTG CACCCCAAAG ATACCAGATA CCAGCACCTG 
AAGGGGAAGA ACGTGATCCA CCCATTCCTG TCTCGGAGCC TTCCCATTGT CTTCGATGAA 
TTTGTGGACA TGGACTTTGG CACAGGTGCT GTGAAGATCA CCCCCGCACA TGACCAAAAT 
GACTATGAAG TTGGGCAGCG GCACGGGCTG GAGGCCATCA GCATCATGGA CTCCCGGGGG 
GCCCTCATCA ATGTGCCTCC GCCTTTCCTG GGCCTGCCCA GGTTTGAGGC CAGGAAAGCG 
GTGCTGGTGG CGCTGAAGGA GCGGGGACTG TTCCGTGGCA TTGAGGACAA CCCCATGGTG 
GTGCCACTTT GCAACCGGTC GAAGGACGTG GTAGAGCCTC TGCTGCGGCC GCAGTGGTAC 
GTTCGCTGCG GGGAGATGGC CCAGGCTGCC AGCGCCGCTG TGACTCGGGG TGACCTCCGC 
ATCCTGCCTG AGGCCCATCA GCGCACATGG CATGCCTGGA TGGACAACAT CCGGGAGTGG 
TGCATTTCCA GGCAGCTGTG GTGGGGCCAT CGCATCCCAG CCTACTTTGT CACTGTCAGT 
GACCCAGCGG TGCCCCCTGG GGAGGACCCT GATGGGCGGT ACTGGGTGAG TGGACGCAAT 
GAGGCGGAGG CCCGGGAGAA GGCAGCCAAG GAGTTCGGAG TGTCCCCTGA CAAGATCAGT 
CTCCAGCAAG ATGAGGATGT ATTGGATACC TGGTTCTCCT CTGGCCTCTT CCCCTTATCC 
ATTTTGGGCT GGCCCAACCA GTCAGAAGAC CTGAGTGTGT TCTACCCCGG GACACTGCTG 
GAGACCGGTC ATGACATCCT CTTCTTCTGG GTGGCCCGGA TGGTCATGCT GGGCCTGAAG 
CTCACGGGCA GGCTGCCCTT TAGAGAGGTC TACCTCCATG CCATCGTGCG AGATGCTCAC 
GGCCGGAAGA TGAGCAAGTC TCTAGGCAAT GTCATCGATC CCCTGGACGT CATCTATGGA 
ATCTCCCTGC AGGGCCTCCA CAACCAGCTG CTGAACAGCA ACCTGGATCC CAGCGAGGTG 
GAGAAGGCCA AAGAAGGGCA GAAAGCTGAC TTCCCAGCGG GGATTCCTGA ATGTGGCACC 
GATGCTCTCC GGTTTGGATT ATGTGCCTAC ATGTCCCAGG GTCGTGACAT CAACCTGGAT 
GTGAACCGGA TACTGGGTTA CCGCCACTTC TGCAACAAGC TCTGGAATGC CACCAAGTTT 
GCCCTTCGTG GCCTTGGGAA GGGTTTTGTG CCCTCACCCA CCTCCCAGCC CGGAGGCCAT 
GAGAGCCTGG TGGACCGCTG GATCCGCAGC CGCCTGACAG AGGCTGTGAG GCTCAGCAAT 
CAAGGCTTCC AGGCCTACGA CTTCCCGGCC GTCACCACTG CCCAGTACAG CTTCTGGCTC 
TATGAGCTCT GTGATGTCTA CTTGGAGTGC CTGAAACCTG TACTGAATGG GGTGGACCAG 
GTGGCAGCTG AGTGTGCCCG CCAGACCCTG TACACTTGCC TGGACGTTGG CCTGCGGCTG 
CTCTCACCCT TCATGCCCTT CGTGACGGAG GAGCTGTTCC AGAGGCTGCC CCGGAGGATG 
CCGCAAGCTC CCCCTAGCCT CTGTGTTACC CCCTACCCGG AGCCCTCAGA GTGCTCCTGG 
AAGGACCCCG AGGCAGAAGC CGCCCTTGAG CTGGCGCTAA GCATCACGCG AGCCGTGCGC 
TCCCTGCGGG CCGACTACAA CCTCACCCGG ATCCGGCCTG ACTGTTTCCT GGAAGTGGCG 
GATGAGGCCA CGGGCGCCCT GGCATCGGCG GTGTCGGGCT ACGTGCAGGC CCTGGCCAGC 
GCAGGTGTGG TGGCTGTTCT GGCCCTGGGG GCTCCCGCCC CCCAGGGTTG CGCTGTGGCT 
CTGGCTTCTG ATCGCTGCTC CATCCACCTG CAGCTTCAGG GGCTGGTGGA CCCTGCACGG 
GAGCTGGGCA AGCTGCAAGC CAAGCGAGTT GAGGCCCAGC GGCAGGCCCA GCGTCTGCGG 
GAACGCCGTG CTGCCTCGGG CTATCCTGTC AAGGTGCCGC TCGAAGTCCA GGAGGCAGAT 
GAAGCCAAGC TCCAACAGAC AGAAGCAGAG CTCAGGAAGG TGGATGAGGC CATCGCCCTA 
TTCCAGAAGA TGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249419.1
CDS	417..4214
Translation	MSTLYVSPHPDAFPSLRALIAARYGEAGEGPGWGGAHPRICLQPPPTSRTPFPPPRLPALEQGPGGLWVWGATAVAQLLWPAGLGGPGGSRAAVLVQQWVSYADTELIPAACGATLPALGLRSSAQDPQAVLGALGRALSPLEEWLRLHTYLAGEAPTLADLAAVTALLLPFRYVLDPPARRIWNNVTRWFVTCVRQPEFRAVLGEVVLYSGARPLSHQPGPEAPALPKTAAQLKKEAKKREKLEKFQQKQKIQQQQPPPGEQKKPKPEKREKRDPGVITYDLPTPPGEKKDVSGPMPDSYSPRYVEAAWYPWWEQQGFFKPEYGRPNVSAANPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLSRHQLGREAFLQEVWKWKEEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSAAVTEAFVRLHEEGIIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDTRYQHLKGKNVIHPFLSRSLPIVFDEFVDMDFGTGAVKITPAHDQNDYEVGQRHGLEAISIMDSRGALINVPPPFLGLPRFEARKAVLVALKERGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIREWCISRQLWWGHRIPAYFVTVSDPAVPPGEDPDGRYWVSGRNEAEAREKAAKEFGVSPDKISLQQDEDVLDTWFSSGLFPLSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGRLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIYGISLQGLHNQLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYMSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSQPGGHESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRMPQAPPSLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASDRCSIHLQLQGLVDPARELGKLQAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	XM_005249362.2
Organism	Homo sapiens(human)
Definition	Homo sapiens valyl-tRNA synthetase 1 (VARS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249362.2

ORF Insert Sequence:

ATGTCCACCC TCTACGTCTC CCCTCACCCA GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
GCCGCTCGCT ATGGGGAGGC TGGGGAGGGT CCCGGATGGG GAGGAGCCCA CCCCCGCATC 
TGTCTCCAGC CACCCCCGAC TAGCAGGACT CCCTTTCCCC CACCCCGCCT GCCGGCCCTG 
GAGCAGGGGC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGGCCCA GCTGCTGTGG 
CCAGCAGGCC TGGGGGGCCC AGGGGGCAGC CGGGCGGCTG TCCTTGTCCA ACAGTGGGTC 
AGTTACGCCG ACACGGAGTT AATACCAGCT GCCTGTGGAG CAACGCTGCC GGCCCTGGGA 
CTCCGAAGCT CGGCCCAGGA CCCCCAGGCT GTGCTGGGGG CCCTGGGCAG GGCCCTGAGC 
CCCTTGGAGG AGTGGCTTCG GCTGCACACC TACTTGGCCG GGGAGGCCCC CACTCTGGCT 
GACCTGGCGG CTGTCACAGC CTTGCTGCTG CCTTTCCGAT ACGTCCTAGA CCCACCTGCC 
CGCCGGATCT GGAATAATGT GACTCGCTGG TTTGTCACGT GTGTCCGGCA GCCAGAATTC 
CGAGCCGTGC TAGGAGAAGT GGTTCTATAC TCAGGAGCCA GGCCTCTCTC TCATCAGCCA 
GGCCCCGAGG CTCCTGCCCT CCCAAAGACA GCTGCTCAGC TCAAGAAAGA GGCAAAGAAA 
CGGGAGAAGC TAGAGAAATT CCAACAGAAG CAGAAGATCC AACAGCAGCA GCCACCTCCA 
GGGGAGCAGA AGAAACCAAA ACCAGAGAAG AGGGAGAAAC GGGATCCTGG GGTCATTACC 
TATGACCTCC CAACCCCACC CGGGGAAAAG AAAGATGTCA GTGGCCCCAT GCCCGACTCC 
TACAGCCCTC GGTATGTGGA GGCTGCCTGG TACCCTTGGT GGGAGCAGCA GGGCTTCTTC 
AAGCCAGAGT ATGGGCGTCC TAATGTGTCA GCAGCAAATC CCCGAGGTGT CTTCATGATG 
TGCATCCCAC CCCCCAATGT GACAGGCTCC CTGCACCTGG GCCATGCACT CACCAACGCC 
ATCCAGGACT CCCTGACTCG ATGGCACCGC ATGCGTGGGG AGACCACCCT GTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCCACCCAG GTGGTGGTGG AGAAGAAGCT ATGGCGTGAG 
CAGGGACTGA GCCGGCACCA GCTGGGCCGC GAGGCCTTTC TACAGGAAGT CTGGAAGTGG 
AAGGAGGAGA AAGGTGACCG GATTTACCAC CAGTTGAAGA AGCTTGGCAG CTCCTTGGAC 
TGGGATCGAG CCTGTTTCAC CATGGACCCT AAACTCTCAG CAGCTGTGAC AGAGGCCTTT 
GTCCGGCTTC ACGAGGAAGG CATCATCTAT CGCAGTACCC GCCTTGTTAA CTGGTCCTGC 
ACCCTCAACT CCGCCATCTC TGACATTGAG GTGGATAAGA AGGAGCTGAC AGGTCGCACC 
CTGCTCTCCG TGCCTGGCTA CAAGGAGAAG GTGGAGTTCG GGGTCCTCGT GTCCTTTGCC 
TATAAGGTCC AAGGCTCAGA TAGCGACGAG GAGGTGGTGG TGGCAACAAC TCGGATCGAG 
ACAATGCTGG GAGATGTGGC TGTAGCTGTG CACCCCAAAG ATACCAGATA CCAGCACCTG 
AAGGGGAAGA ACGTGATCCA CCCATTCCTG TCTCGGAGCC TTCCCATTGT CTTCGATGAA 
TTTGTGGACA TGGACTTTGG CACAGGTGCT GTGAAGATCA CCCCCGCACA TGACCAAAAT 
GACTATGAAG TTGGGCAGCG GCACGGGCTG GAGGCCATCA GCATCATGGA CTCCCGGGGG 
GCCCTCATCA ATGTGCCTCC GCCTTTCCTG GGCCTGCCCA GGTTTGAGGC CAGGAAAGCG 
GTGCTGGTGG CGCTGAAGGA GCGGGGACTG TTCCGTGGCA TTGAGGACAA CCCCATGGTG 
GTGCCACTTT GCAACCGGTC GAAGGACGTG GTAGAGCCTC TGCTGCGGCC GCAGTGGTAC 
GTTCGCTGCG GGGAGATGGC CCAGGCTGCC AGCGCCGCTG TGACTCGGGG TGACCTCCGC 
ATCCTGCCTG AGGCCCATCA GCGCACATGG CATGCCTGGA TGGACAACAT CCGGGAGTGG 
TGCATTTCCA GGCAGCTGTG GTGGGGCCAT CGCATCCCAG CCTACTTTGT CACTGTCAGT 
GACCCAGCGG TGCCCCCTGG GGAGGACCCT GATGGGCGGT ACTGGGTGAG TGGACGCAAT 
GAGGCGGAGG CCCGGGAGAA GGCAGCCAAG GAGTTCGGAG TGTCCCCTGA CAAGATCAGT 
CTCCAGCAAG ATGAGGATGT ATTGGATACC TGGTTCTCCT CTGGCCTCTT CCCCTTATCC 
ATTTTGGGCT GGCCCAACCA GTCAGAAGAC CTGAGTGTGT TCTACCCCGG GACACTGCTG 
GAGACCGGTC ATGACATCCT CTTCTTCTGG GTGGCCCGGA TGGTCATGCT GGGCCTGAAG 
CTCACGGGCA GGCTGCCCTT TAGAGAGGTC TACCTCCATG CCATCGTGCG AGATGCTCAC 
GGCCGGAAGA TGAGCAAGTC TCTAGGCAAT GTCATCGATC CCCTGGACGT CATCTATGGA 
ATCTCCCTGC AGGGCCTCCA CAACCAGCTG CTGAACAGCA ACCTGGATCC CAGCGAGGTG 
GAGAAGGCCA AAGAAGGGCA GAAAGCTGAC TTCCCAGCGG GGATTCCTGA ATGTGGCACC 
GATGCTCTCC GGTTTGGATT ATGTGCCTAC ATGTCCCAGG GTCGTGACAT CAACCTGGAT 
GTGAACCGGA TACTGGGTTA CCGCCACTTC TGCAACAAGC TCTGGAATGC CACCAAGTTT 
GCCCTTCGTG GCCTTGGGAA GGGTTTTGTG CCCTCACCCA CCTCCCAGCC CGGAGGCCAT 
GAGAGCCTGG TGGACCGCTG GATCCGCAGC CGCCTGACAG AGGCTGTGAG GCTCAGCAAT 
CAAGGCTTCC AGGCCTACGA CTTCCCGGCC GTCACCACTG CCCAGTACAG CTTCTGGCTC 
TATGAGCTCT GTGATGTCTA CTTGGAGTGC CTGAAACCTG TACTGAATGG GGTGGACCAG 
GTGGCAGCTG AGTGTGCCCG CCAGACCCTG TACACTTGCC TGGACGTTGG CCTGCGGCTG 
CTCTCACCCT TCATGCCCTT CGTGACGGAG GAGCTGTTCC AGAGGCTGCC CCGGAGGATG 
CCGCAAGCTC CCCCTAGCCT CTGTGTTACC CCCTACCCGG AGCCCTCAGA GTGCTCCTGG 
AAGGACCCCG AGGCAGAAGC CGCCCTTGAG CTGGCGCTAA GCATCACGCG AGCCGTGCGC 
TCCCTGCGGG CCGACTACAA CCTCACCCGG ATCCGGCCTG ACTGTTTCCT GGAAGTGGCG 
GATGAGGCCA CGGGCGCCCT GGCATCGGCG GTGTCGGGCT ACGTGCAGGC CCTGGCCAGC 
GCAGGTGTGG TGGCTGTTCT GGCCCTGGGG GCTCCCGCCC CCCAGGGTTG CGCTGTGGCT 
CTGGCTTCTG ATCGCTGCTC CATCCACCTG CAGCTTCAGG GGCTGGTGGA CCCTGCACGG 
GAGCTGGGCA AGCTGCAAGC CAAGCGAGTT GAGGCCCAGC GGCAGGCCCA GCGTCTGCGG 
GAACGCCGTG CTGCCTCGGG CTATCCTGTC AAGGTGCCGC TCGAAGTCCA GGAGGCAGAT 
GAAGCCAAGC TCCAACAGAC AGAAGCAGAG CTCAGGAAGG TGGATGAGGC CATCGCCCTA 
TTCCAGAAGA TGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Journal of virology88(18)10340-53(2014 Sep)
Jefferson M,Donaszi-Ivanov A,Pollen S,Dalmay T,Saalbach G,Powell PP

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
American journal of human genetics85(5)628-42(2009 Nov)
Talmud PJ,Drenos F,Shah S,Shah T,Palmen J,Verzilli C,Gaunt TR,Pallas J,Lovering R,Li K,Casas JP,Sofat R,Kumari M,Rodriguez S,Johnson T,Newhouse SJ,Dominiczak A,Samani NJ,Caulfield M,Sever P,Stanton A,Shields DC,Padmanabhan S,Melander O,Hastie C,Delles C,Ebrahim S,Marmot MG,Smith GD,Lawlor DA,Munroe PB,Day IN,Kivimaki M,Whittaker J,Humphries SE,Hingorani AD,,,

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VARS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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FAX

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