BTF3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BTF3
Entrez Gene ID	689
Full Name	basic transcription factor 3
Synonyms	BETA-NAC,BTF3a,BTF3b,NACB
General protein information	Preferred Names basic transcription factor 3 Names transcription factor BTF3 NAC-beta RNA polymerase B transcription factor 3 nascent polypeptide-associated complex subunit beta nascent-polypeptide-associated complex beta polypeptide
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q13.2
Summary	This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008].
Disorder MIM:	602542

mRNA and Protein(s)

mRNA	Protein	Name
NM_001207.4	NP_001198.2	transcription factor BTF3 isoform B
NM_001037637.1	NP_001032726.1	transcription factor BTF3 isoform A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Btf3	NP_001008310.1
Arabidopsis thaliana (thale cress)	AT1G73230	NP_177466.1
	BTF3	XP_001100404.1
Canis lupus familiaris (dog)	BTF3	NP_001239062.1
Danio rerio (zebrafish)	btf3	NP_001070026.1
Drosophila melanogaster (fruit fly)	CG11835	NP_608532.1
Oryza sativa (rice)	Os03g0851000	NP_001051911.1
Homo sapiens (human)	BTF3	NP_001032726.1
Pan troglodytes (chimpanzee)	BTF3	XP_517710.1
Bos taurus (cattle)	BTF3	NP_001020485.1
Gallus gallus (chicken)	BTF3	XP_423823.4
Mus musculus (house mouse)	Btf3	NP_663430.2
Drosophila melanogaster (fruit fly)	bic	NP_725235.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006614	XP_316643.3
Arabidopsis thaliana (thale cress)	BTF3	NP_173230.1
Xenopus tropicalis (tropical clawed frog)	btf3	NP_001016697.1

Gene Ontology
Bibliography

Related articles in PubMed

Basic Transcription Factor 3 Is Required for Proliferation and Epithelial-Mesenchymal Transition via Regulation of FOXM1 and JAK2/STAT3 Signaling in Gastric Cancer.
Zhang DZ, Chen BH, Zhang LF, Cheng MK, Fang XJ, Wu XJ
Oncology research25(9)1453-1462(2017 Nov)

Quantitative analysis of BTF3, HINT1, NDRG1 and ODC1 protein over-expression in human prostate cancer tissue.
Symes AJ, Eilertsen M, Millar M, Nariculam J, Freeman A, Notara M, Feneley MR, Patel HR, Patel HR, Masters JR, Ahmed A
PloS one8(12)e84295(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BTF3 overexpression may be an early event in colorectal cancer development and could be useful biomarker for the early stage of colorectal cancers (CRC); BTF3 has positive correlations with NF-kappaB, RAD50, MRE11, NBS1 and AEG-1 and might influence complex signal pathways in CRC
Title: Clinicopathological significance of BTF3 expression in colorectal cancer.

BTF3 expression is associated with enhanced cell proliferation, reduced cell cycle regulation and apoptosis and its silencing decreased colony forming and proliferation of gastric cancer cells.
Title: Basic transcription factor 3 is involved in gastric cancer development and progression.

BTF3, HINT1, NDRG1 and ODC1 protein over-expression in human prostate cancer tissue
Title: Quantitative analysis of BTF3, HINT1, NDRG1 and ODC1 protein over-expression in human prostate cancer tissue.

may have a functional role in protecting against caries
Title: Fine-mapping of 5q12.1-13.3 unveils new genetic contributors to caries.

BTF3 interacts with ERalpha that has been activated either by 17beta-estradiol (ligand-dependent activation) or by epidermal growth factor (ligand-independent activation).
Title: Interaction between transcription factor, basal transcription factor 3, and the NH2-terminal domain of human estrogen receptor alpha.

The following BTF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BTF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu11292	NM_001207.4 Latest version!	Homo sapiens basic transcription factor 3 (BTF3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10886	NM_001037637.1 Latest version!	Homo sapiens basic transcription factor 3 (BTF3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BTF3 ( NM_001207.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001198.2 Homo sapiens basic transcription factor 3 (BTF3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu11292
Clone ID Related Accession (Same CDS sequence)	NM_001207.4
Accession Version	NM_001207.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	transcription factor BTF3 isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU661989.1, BC008062.2 and BG182166.1. On Dec 14, 2005 this sequence version replaced NM_001207.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in translation initiation at a downstream AUG and a protein (isoform B) with a shorter N-terminus, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.190366.1, SRR1163657.578911.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAAGAAA CAATCATGAA CCAGGAAAAA CTCGCCAAAC TGCAGGCACA AGTGCGCATT 
GGTGGGAAAG GAACTGCTCG CAGAAAGAAG AAGGTGGTTC ATAGAACAGC CACAGCAGAT 
GACAAAAAAC TTCAGTTCTC CTTAAAGAAG TTAGGGGTAA ACAATATCTC TGGTATTGAA 
GAGGTGAATA TGTTTACAAA CCAAGGAACA GTGATCCACT TTAACAACCC TAAAGTTCAG 
GCATCTCTGG CAGCGAACAC TTTCACCATT ACAGGCCATG CTGAGACAAA GCAGCTGACA 
GAAATGCTAC CCAGCATCTT AAACCAGCTT GGTGCGGATA GTCTGACTAG TTTAAGGAGA 
CTGGCCGAAG CTCTGCCCAA ACAATCTGTG GATGGAAAAG CACCACTTGC TACTGGAGAG 
GATGATGATG ATGAAGTTCC AGATCTTGTG GAGAATTTTG ATGAGGCTTC CAAGAATGAG 
GCAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001198.2
CDS	135..623
Translation	MKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNEAN

Target ORF information:

RefSeq Version	NM_001207.4
Organism	Homo sapiens(human)
Definition	Homo sapiens basic transcription factor 3 (BTF3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001207.4

ORF Insert Sequence:

ATGAAAGAAA CAATCATGAA CCAGGAAAAA CTCGCCAAAC TGCAGGCACA AGTGCGCATT 
GGTGGGAAAG GAACTGCTCG CAGAAAGAAG AAGGTGGTTC ATAGAACAGC CACAGCAGAT 
GACAAAAAAC TTCAGTTCTC CTTAAAGAAG TTAGGGGTAA ACAATATCTC TGGTATTGAA 
GAGGTGAATA TGTTTACAAA CCAAGGAACA GTGATCCACT TTAACAACCC TAAAGTTCAG 
GCATCTCTGG CAGCGAACAC TTTCACCATT ACAGGCCATG CTGAGACAAA GCAGCTGACA 
GAAATGCTAC CCAGCATCTT AAACCAGCTT GGTGCGGATA GTCTGACTAG TTTAAGGAGA 
CTGGCCGAAG CTCTGCCCAA ACAATCTGTG GATGGAAAAG CACCACTTGC TACTGGAGAG 
GATGATGATG ATGAAGTTCC AGATCTTGTG GAGAATTTTG ATGAGGCTTC CAAGAATGAG 
GCAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTF3 ( NM_001037637.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001032726.1 Homo sapiens basic transcription factor 3 (BTF3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10886
Clone ID Related Accession (Same CDS sequence)	NM_001037637.1
Accession Version	NM_001037637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	transcription factor BTF3 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU661989.1, BX359147.2 and BG182166.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.44725.1, SRR1163657.196167.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGACGGA CAGGCGCACC CGCTCAGGCT GACTCTCGGG GGCGAGGTCG AGCCAGGGGC 
GGCTGCCCTG GGGGCGAGGC GACGCTGTCT CAACCTCCAC CTCGCGGCGG AACCCGAGGA 
CAGGAGCCTC AGATGAAAGA AACAATCATG AACCAGGAAA AACTCGCCAA ACTGCAGGCA 
CAAGTGCGCA TTGGTGGGAA AGGAACTGCT CGCAGAAAGA AGAAGGTGGT TCATAGAACA 
GCCACAGCAG ATGACAAAAA ACTTCAGTTC TCCTTAAAGA AGTTAGGGGT AAACAATATC 
TCTGGTATTG AAGAGGTGAA TATGTTTACA AACCAAGGAA CAGTGATCCA CTTTAACAAC 
CCTAAAGTTC AGGCATCTCT GGCAGCGAAC ACTTTCACCA TTACAGGCCA TGCTGAGACA 
AAGCAGCTGA CAGAAATGCT ACCCAGCATC TTAAACCAGC TTGGTGCGGA TAGTCTGACT 
AGTTTAAGGA GACTGGCCGA AGCTCTGCCC AAACAATCTG TGGATGGAAA AGCACCACTT 
GCTACTGGAG AGGATGATGA TGATGAAGTT CCAGATCTTG TGGAGAATTT TGATGAGGCT 
TCCAAGAATG AGGCAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032726.1
CDS	244..864
Translation	MRRTGAPAQADSRGRGRARGGCPGGEATLSQPPPRGGTRGQEPQMKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNEAN

Target ORF information:

RefSeq Version	NM_001037637.1
Organism	Homo sapiens(human)
Definition	Homo sapiens basic transcription factor 3 (BTF3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037637.1

ORF Insert Sequence:

ATGCGACGGA CAGGCGCACC CGCTCAGGCT GACTCTCGGG GGCGAGGTCG AGCCAGGGGC 
GGCTGCCCTG GGGGCGAGGC GACGCTGTCT CAACCTCCAC CTCGCGGCGG AACCCGAGGA 
CAGGAGCCTC AGATGAAAGA AACAATCATG AACCAGGAAA AACTCGCCAA ACTGCAGGCA 
CAAGTGCGCA TTGGTGGGAA AGGAACTGCT CGCAGAAAGA AGAAGGTGGT TCATAGAACA 
GCCACAGCAG ATGACAAAAA ACTTCAGTTC TCCTTAAAGA AGTTAGGGGT AAACAATATC 
TCTGGTATTG AAGAGGTGAA TATGTTTACA AACCAAGGAA CAGTGATCCA CTTTAACAAC 
CCTAAAGTTC AGGCATCTCT GGCAGCGAAC ACTTTCACCA TTACAGGCCA TGCTGAGACA 
AAGCAGCTGA CAGAAATGCT ACCCAGCATC TTAAACCAGC TTGGTGCGGA TAGTCTGACT 
AGTTTAAGGA GACTGGCCGA AGCTCTGCCC AAACAATCTG TGGATGGAAA AGCACCACTT 
GCTACTGGAG AGGATGATGA TGATGAAGTT CCAGATCTTG TGGAGAATTT TGATGAGGCT 
TCCAAGAATG AGGCAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Basic Transcription Factor 3 Is Required for Proliferation and Epithelial-Mesenchymal Transition via Regulation of FOXM1 and JAK2/STAT3 Signaling in Gastric Cancer.
Oncology research25(9)1453-1462(2017 Nov)
Zhang DZ,Chen BH,Zhang LF,Cheng MK,Fang XJ,Wu XJ

Quantitative analysis of BTF3, HINT1, NDRG1 and ODC1 protein over-expression in human prostate cancer tissue.
PloS one8(12)e84295(2013)
Symes AJ,Eilertsen M,Millar M,Nariculam J,Freeman A,Notara M,Feneley MR,Patel HR,Patel HR,Masters JR,Ahmed A

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BTF3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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電話

FAX

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