SPR cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPR
Entrez Gene ID	6697
Full Name	sepiapterin reductase
Synonyms	SDR38C1
General protein information	Preferred Names sepiapterin reductase Names sepiapterin reductase Sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming) sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) short chain dehydrogenase/reductase family 38C, member 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p13.2
Summary	This gene encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1. [provided by RefSeq, Jul 2008].
Disorder MIM:	182125
Disorder Html:	Dystonia, dopa-responsive, due to sepiapterin reductase deficiency, 612716 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_003124.4	NP_003115.1	sepiapterin reductase
NM_003124.5	NP_003115.1	sepiapterin reductase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPR	XP_525784.2
Mus musculus (house mouse)	Spr	NP_035597.2
Danio rerio (zebrafish)	spra	NP_001019601.2
Canis lupus familiaris (dog)	SPR	XP_540234.2
Gallus gallus (chicken)	SPR	XP_423038.1
Danio rerio (zebrafish)	sprb	NP_001122262.1
Drosophila melanogaster (fruit fly)	Sptr	NP_727265.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008000	XP_317468.3
	SPR	XP_001103332.1
Rattus norvegicus (Norway rat)	Spr	NP_062054.1
Homo sapiens (human)	SPR	NP_003115.1
Bos taurus (cattle)	SPR	NP_001069471.1
Xenopus tropicalis (tropical clawed frog)	spr	NP_001120067.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic causes of Parkinson's disease in the Maltese: a study of selected mutations in LRRK2, MTHFR, QDPR and SPR.
Zahra C, Tabone C, Camilleri G, Felice AE, Farrugia R, Bezzina Wettinger S
BMC medical genetics17(1)65(2016 09)

Effect of sulfasalazine on human neuroblastoma: analysis of sepiapterin reductase (SPR) as a new therapeutic target.
Yco LP, Geerts D, Mocz G, Koster J, Bachmann AS
BMC cancer15477(2015 Jun)

Novel interaction of ornithine decarboxylase with sepiapterin reductase regulates neuroblastoma cell proliferation.
Lange I, Geerts D, Feith DJ, Mocz G, Koster J, Bachmann AS
Journal of molecular biology426(2)332-46(2014 Jan)

Clinical and genetic studies in a family with a novel mutation in the sepiapterin reductase gene.
Koht J, Rengmark A, Opladen T, Bj?rnar? KA, Selberg T, Tallaksen CM, Blau N, Toft M
Acta neurologica Scandinavica. Supplementum(198)7-12(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The allele frequencies for the SPR c.596-2A > G (0.7%) polymorphism is not a major cause of Parkinson's disease in the Maltese.
Title: Genetic causes of Parkinson's disease in the Maltese: a study of selected mutations in LRRK2, MTHFR, QDPR and SPR.

We earlier presented evidence for a physical interaction between ODC and SPR and we showed that RNAi-mediated knockdown of SPR expression significantly reduced native ODC enzyme activity and impeded Neuroblastoma cell proliferation.
Title: Effect of sulfasalazine on human neuroblastoma: analysis of sepiapterin reductase (SPR) as a new therapeutic target.

Authors identified SPR as a novel regulator of ODC enzyme activity and, based on clinical evidence, present a model in which SPR drives ODC-mediated malignant progression in neuroblastoma.
Title: Novel interaction of ornithine decarboxylase with sepiapterin reductase regulates neuroblastoma cell proliferation.

new homozygous mutation in the SPR gene was found in two sisters with dopa-responsive dystonia
Title: Clinical and genetic studies in a family with a novel mutation in the sepiapterin reductase gene.

SPR-mediated reduction of sepiapterin and redox cycling occur by distinct mechanisms
Title: Sepiapterin reductase mediates chemical redox cycling in lung epithelial cells.

The following SPR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26401	NM_003124.5 Latest version!	Homo sapiens sepiapterin reductase (SPR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu26401	NM_003124.4	Homo sapiens sepiapterin reductase (SPR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPR ( NM_003124.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003115.1 Homo sapiens sepiapterin reductase (SPR), mRNA.

Price & Availability↑

CloneID	OHu26401
Clone ID Related Accession (Same CDS sequence)	NM_003124.4 , NM_003124.5
Accession Version	NM_003124.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	sepiapterin reductase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092630.3, BC017310.2 and BF434649.1. This sequence is a reference standard in the RefSeqGene project. On Mar 7, 2008 this sequence version replaced NM_003124.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222942.1, BC017310.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGCG GGCTGGGGCG TGCTGTGTGC TTGCTGACCG GGGCCTCCCG CGGCTTCGGC 
CGGACGCTGG CCCCGCTCCT GGCCTCGCTG CTGTCGCCCG GCTCCGTGCT TGTCCTTAGC 
GCCCGCAACG ACGAGGCACT GCGCCAGCTG GAGGCCGAGC TGGGCGCCGA GCGGTCTGGC 
CTGCGCGTGG TGCGGGTGCC CGCCGACCTG GGCGCCGAGG CCGGCTTGCA GCAGCTGCTC 
GGCGCCCTGC GCGAGCTCCC CCGGCCCAAG GGGCTGCAGC GACTGCTGCT TATCAACAAC 
GCGGGCTCTC TTGGGGATGT GTCCAAAGGC TTCGTGGACC TGAGTGACTC CACTCAAGTG 
AACAACTACT GGGCACTGAA CTTGACCTCC ATGCTCTGCC TGACTTCCAG CGTCCTGAAG 
GCCTTCCCGG ACAGTCCTGG CCTCAACAGA ACCGTGGTTA ACATCTCGTC CCTCTGTGCC 
CTGCAACCTT TCAAAGGCTG GGCGCTGTAC TGTGCAGGAA AGGCTGCTCG TGATATGCTG 
TTCCAGGTCC TGGCGCTGGA GGAACCTAAT GTGAGGGTGC TGAACTATGC CCCAGGTCCT 
CTGGACACAG ACATGCAGCA GTTGGCCCGG GAGACCTCCG TGGACCCAGA CATGCGAAAA 
GGGCTGCAGG AGCTGAAGGC AAAGGGGAAG CTGGTGGATT GCAAGGTGTC AGCCCAGAAA 
CTGCTGAGCT TACTGGAAAA GGACGAGTTC AAGTCTGGAG CCCACGTGGA CTTCTATGAC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003115.1
CDS	51..836
Translation	MEGGLGRAVCLLTGASRGFGRTLAPLLASLLSPGSVLVLSARNDEALRQLEAELGAERSGLRVVRVPADLGAEAGLQQLLGALRELPRPKGLQRLLLINNAGSLGDVSKGFVDLSDSTQVNNYWALNLTSMLCLTSSVLKAFPDSPGLNRTVVNISSLCALQPFKGWALYCAGKAARDMLFQVLALEEPNVRVLNYAPGPLDTDMQQLARETSVDPDMRKGLQELKAKGKLVDCKVSAQKLLSLLEKDEFKSGAHVDFYDK

Target ORF information:

RefSeq Version	NM_003124.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sepiapterin reductase (SPR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003124.4

ORF Insert Sequence:

ATGGAGGGCG GGCTGGGGCG TGCTGTGTGC TTGCTGACCG GGGCCTCCCG CGGCTTCGGC 
CGGACGCTGG CCCCGCTCCT GGCCTCGCTG CTGTCGCCCG GCTCCGTGCT TGTCCTTAGC 
GCCCGCAACG ACGAGGCACT GCGCCAGCTG GAGGCCGAGC TGGGCGCCGA GCGGTCTGGC 
CTGCGCGTGG TGCGGGTGCC CGCCGACCTG GGCGCCGAGG CCGGCTTGCA GCAGCTGCTC 
GGCGCCCTGC GCGAGCTCCC CCGGCCCAAG GGGCTGCAGC GACTGCTGCT TATCAACAAC 
GCGGGCTCTC TTGGGGATGT GTCCAAAGGC TTCGTGGACC TGAGTGACTC CACTCAAGTG 
AACAACTACT GGGCACTGAA CTTGACCTCC ATGCTCTGCC TGACTTCCAG CGTCCTGAAG 
GCCTTCCCGG ACAGTCCTGG CCTCAACAGA ACCGTGGTTA ACATCTCGTC CCTCTGTGCC 
CTGCAACCTT TCAAAGGCTG GGCGCTGTAC TGTGCAGGAA AGGCTGCTCG TGATATGCTG 
TTCCAGGTCC TGGCGCTGGA GGAACCTAAT GTGAGGGTGC TGAACTATGC CCCAGGTCCT 
CTGGACACAG ACATGCAGCA GTTGGCCCGG GAGACCTCCG TGGACCCAGA CATGCGAAAA 
GGGCTGCAGG AGCTGAAGGC AAAGGGGAAG CTGGTGGATT GCAAGGTGTC AGCCCAGAAA 
CTGCTGAGCT TACTGGAAAA GGACGAGTTC AAGTCTGGAG CCCACGTGGA CTTCTATGAC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPR ( NM_003124.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003115.1 Homo sapiens sepiapterin reductase (SPR), mRNA.

Price & Availability↑

CloneID	OHu26401
Clone ID Related Accession (Same CDS sequence)	NM_003124.4 , NM_003124.5
Accession Version	NM_003124.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	sepiapterin reductase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092630.3, BC017310.2 and BF434649.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_003124.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.467140.1, AK222942.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000234454.6/ ENSP00000234454.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGGCG GGCTGGGGCG TGCTGTGTGC TTGCTGACCG GGGCCTCCCG CGGCTTCGGC 
CGGACGCTGG CCCCGCTCCT GGCCTCGCTG CTGTCGCCCG GCTCCGTGCT TGTCCTTAGC 
GCCCGCAACG ACGAGGCACT GCGCCAGCTG GAGGCCGAGC TGGGCGCCGA GCGGTCTGGC 
CTGCGCGTGG TGCGGGTGCC CGCCGACCTG GGCGCCGAGG CCGGCTTGCA GCAGCTGCTC 
GGCGCCCTGC GCGAGCTCCC CCGGCCCAAG GGGCTGCAGC GACTGCTGCT TATCAACAAC 
GCGGGCTCTC TTGGGGATGT GTCCAAAGGC TTCGTGGACC TGAGTGACTC CACTCAAGTG 
AACAACTACT GGGCACTGAA CTTGACCTCC ATGCTCTGCC TGACTTCCAG CGTCCTGAAG 
GCCTTCCCGG ACAGTCCTGG CCTCAACAGA ACCGTGGTTA ACATCTCGTC CCTCTGTGCC 
CTGCAACCTT TCAAAGGCTG GGCGCTGTAC TGTGCAGGAA AGGCTGCTCG TGATATGCTG 
TTCCAGGTCC TGGCGCTGGA GGAACCTAAT GTGAGGGTGC TGAACTATGC CCCAGGTCCT 
CTGGACACAG ACATGCAGCA GTTGGCCCGG GAGACCTCCG TGGACCCAGA CATGCGAAAA 
GGGCTGCAGG AGCTGAAGGC AAAGGGGAAG CTGGTGGATT GCAAGGTGTC AGCCCAGAAA 
CTGCTGAGCT TACTGGAAAA GGACGAGTTC AAGTCTGGAG CCCACGTGGA CTTCTATGAC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003115.1
CDS	26..811
Translation	MEGGLGRAVCLLTGASRGFGRTLAPLLASLLSPGSVLVLSARNDEALRQLEAELGAERSGLRVVRVPADLGAEAGLQQLLGALRELPRPKGLQRLLLINNAGSLGDVSKGFVDLSDSTQVNNYWALNLTSMLCLTSSVLKAFPDSPGLNRTVVNISSLCALQPFKGWALYCAGKAARDMLFQVLALEEPNVRVLNYAPGPLDTDMQQLARETSVDPDMRKGLQELKAKGKLVDCKVSAQKLLSLLEKDEFKSGAHVDFYDK

Target ORF information:

RefSeq Version	NM_003124.5
Organism	Homo sapiens(human)
Definition	Homo sapiens sepiapterin reductase (SPR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003124.5

ORF Insert Sequence:

ATGGAGGGCG GGCTGGGGCG TGCTGTGTGC TTGCTGACCG GGGCCTCCCG CGGCTTCGGC 
CGGACGCTGG CCCCGCTCCT GGCCTCGCTG CTGTCGCCCG GCTCCGTGCT TGTCCTTAGC 
GCCCGCAACG ACGAGGCACT GCGCCAGCTG GAGGCCGAGC TGGGCGCCGA GCGGTCTGGC 
CTGCGCGTGG TGCGGGTGCC CGCCGACCTG GGCGCCGAGG CCGGCTTGCA GCAGCTGCTC 
GGCGCCCTGC GCGAGCTCCC CCGGCCCAAG GGGCTGCAGC GACTGCTGCT TATCAACAAC 
GCGGGCTCTC TTGGGGATGT GTCCAAAGGC TTCGTGGACC TGAGTGACTC CACTCAAGTG 
AACAACTACT GGGCACTGAA CTTGACCTCC ATGCTCTGCC TGACTTCCAG CGTCCTGAAG 
GCCTTCCCGG ACAGTCCTGG CCTCAACAGA ACCGTGGTTA ACATCTCGTC CCTCTGTGCC 
CTGCAACCTT TCAAAGGCTG GGCGCTGTAC TGTGCAGGAA AGGCTGCTCG TGATATGCTG 
TTCCAGGTCC TGGCGCTGGA GGAACCTAAT GTGAGGGTGC TGAACTATGC CCCAGGTCCT 
CTGGACACAG ACATGCAGCA GTTGGCCCGG GAGACCTCCG TGGACCCAGA CATGCGAAAA 
GGGCTGCAGG AGCTGAAGGC AAAGGGGAAG CTGGTGGATT GCAAGGTGTC AGCCCAGAAA 
CTGCTGAGCT TACTGGAAAA GGACGAGTTC AAGTCTGGAG CCCACGTGGA CTTCTATGAC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic causes of Parkinson's disease in the Maltese: a study of selected mutations in LRRK2, MTHFR, QDPR and SPR.
BMC medical genetics17(1)65(2016 09)
Zahra C,Tabone C,Camilleri G,Felice AE,Farrugia R,Bezzina Wettinger S

Effect of sulfasalazine on human neuroblastoma: analysis of sepiapterin reductase (SPR) as a new therapeutic target.
BMC cancer15477(2015 Jun)
Yco LP,Geerts D,Mocz G,Koster J,Bachmann AS

Novel interaction of ornithine decarboxylase with sepiapterin reductase regulates neuroblastoma cell proliferation.
Journal of molecular biology426(2)332-46(2014 Jan)
Lange I,Geerts D,Feith DJ,Mocz G,Koster J,Bachmann AS

Clinical and genetic studies in a family with a novel mutation in the sepiapterin reductase gene.
Acta neurologica Scandinavica. Supplementum(198)7-12(2014)
Koht J,Rengmark A,Opladen T,Bj?rnar? KA,Selberg T,Tallaksen CM,Blau N,Toft M