ENGASE cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ENGASE
Entrez Gene ID	64772
Full Name	endo-beta-N-acetylglucosaminidase
General protein information	Preferred Names endo-beta-N-acetylglucosaminidase Names cytosolic endo-beta-N-acetylglucosaminidase Di-N-acetylchitobiosyl beta-N-acetylglucosaminidase Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q25.3
Summary	This gene encodes a cytosolic enzyme which catalyzes the hydrolysis of peptides and proteins with mannose modifications to produce free oligosaccharides. [provided by RefSeq, Feb 2012].
Disorder MIM:	611898

mRNA and Protein(s)

mRNA	Protein	Name
XM_017024961.1	XP_016880450.1	cytosolic endo-beta-N-acetylglucosaminidase isoform X1
XM_006722017.3	XP_006722080.1	cytosolic endo-beta-N-acetylglucosaminidase isoform X2
NM_001042573.2	NP_001036038.1	cytosolic endo-beta-N-acetylglucosaminidase
XM_006722019.3	XP_006722082.1	cytosolic endo-beta-N-acetylglucosaminidase isoform X4
NM_001042573.3	NP_001036038.1	cytosolic endo-beta-N-acetylglucosaminidase
XM_006722018.3	XP_006722081.1	cytosolic endo-beta-N-acetylglucosaminidase isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ENGASE	XP_001159278.1
Canis lupus familiaris (dog)	ENGASE	XP_005624210.1
Rattus norvegicus (Norway rat)	Engase	NP_001100540.1
Danio rerio (zebrafish)	engase	NP_001074047.2
Homo sapiens (human)	ENGASE	NP_001036038.1
Gallus gallus (chicken)	ENGASE	XP_427660.4
Arabidopsis thaliana (thale cress)	AT3G11040	NP_187715.1
Oryza sativa (rice)	Os05g0346500	NP_001055263.2
Xenopus tropicalis (tropical clawed frog)	engase	XP_002932564.2
Arabidopsis thaliana (thale cress)	AT5G05460	NP_196165.3
Mus musculus (house mouse)	Engase	NP_766161.1
Bos taurus (cattle)	ENGASE	XP_005221346.1

Gene Ontology
Bibliography

Related articles in PubMed

Relationship of urinary endothelin-1 with estimated glomerular filtration rate in autosomal dominant polycystic kidney disease: a pilot cross-sectional analysis.
Raina R, Lou L, Berger B, Vogt B, Do AS, Cunningham R, Vasavada P, Herrmann K, Dell K, Simonson M
BMC nephrology1722(2016 Feb)

Telomere dysfunction-related serological markers are associated with type 2 diabetes.
Xiao F, Zheng X, Cui M, Shi G, Chen X, Li R, Song Z, Rudolph KL, Chen B, Ju Z
Diabetes care34(10)2273-8(2011 Oct)

Increased expression of beta-N-acetylglucosaminidase in erythrocytes from individuals with pre-diabetes and diabetes.
Park K, Saudek CD, Hart GW
Diabetes59(7)1845-50(2010 Jul)

Identification of roles for peptide: N-glycanase and endo-beta-N-acetylglucosaminidase (Engase1p) during protein N-glycosylation in human HepG2 cells.
Chantret I, Fasseu M, Zaoui K, Le Bizec C, Sadou Yay? H, Dupr? T, Moore SE
PloS one5(7)e11734(2010 Jul)

Screening-based discovery of drug-like O-GlcNAcase inhibitor scaffolds.
Dorfmueller HC, van Aalten DM
FEBS letters584(4)694-700(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In patients with autosomal dominant polycystic kidney disease, urinary NAGase was correlated with urinary ET-1 which was inversely associated with eGFR and positively correlated with total kidney volume.
Title: Relationship of urinary endothelin-1 with estimated glomerular filtration rate in autosomal dominant polycystic kidney disease: a pilot cross-sectional analysis.

Serological N-acetyl-glucosaminidase, telomere length, and the UCP2-886G>A variant are independent risk factors for type 2 diabetes.
Title: Telomere dysfunction-related serological markers are associated with type 2 diabetes.

O-GlcNAcase expression is increased in erythrocytes from both individuals with pre-diabetes and individuals with less well-controlled diabetes.
Title: Increased expression of beta-N-acetylglucosaminidase in erythrocytes from individuals with pre-diabetes and diabetes.

As the generation of the bulk of fOS is unaffected by co-down regulation of Ngly1p and Engase1p, alternative quantitatively important mechanisms must underlie the liberation of these fOS from either LLO or glycoproteins during protein N-glycosylation.
Title: Identification of roles for peptide: N-glycanase and endo-beta-N-acetylglucosaminidase (Engase1p) during protein N-glycosylation in human HepG2 cells.

Data show that kinetic and X-ray crystallographic analyses of the binding modes with human/bacterial O-GlcNAcases identify some of these as competitive inhibitors.
Title: Screening-based discovery of drug-like O-GlcNAcase inhibitor scaffolds.

The following ENGASE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ENGASE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu50588	XM_017024961.1 Latest version!	Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu50589	XM_006722017.3 Latest version!	Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu50590	XM_006722018.3 Latest version!	Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu00159	NM_001042573.3 Latest version!	Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu50591	XM_006722019.3 Latest version!	Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu00159	NM_001042573.2	Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$524.30 $749.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ENGASE ( XM_017024961.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880450.1 Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu50588
Clone ID Related Accession (Same CDS sequence)	XM_017024961.1
Accession Version	XM_017024961.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cytosolic endo-beta-N-acetylglucosaminidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGCG GGTACCTGGA TGACAGGTTC ATTCAGGGCT CGGTGGTGCA GACTCCCTAT 
GCTTTCTACC ACTGGCAGTG CATCGACGTC TTTGTGTACT TCAGCCACCA CACCGTCACC 
ATTCCCCCAG TGGGCTGGAC CAACACTGCC CACAGGCATG GGGTCTGCGT GCTGGGGACT 
TTCATCACGG AGTGGAATGA AGGGGGAAGG CTCTGTGAAG CCTTCCTGGC CGGGGATGAG 
CGCTCGTACC AGGCAGTGGC TGACCGGCTG GTCCAGATCA CTCAGTTTTT TCGTTTTGAT 
GGCTGGCTGA TCAACATCGA GAACTCGCTG AGTCTGGCCG CTGTGGGGAA CATGCCTCCT 
TTCCTGCGGT ACCTCACCAC ACAGCTGCAC CGGCAGGTCC CAGGGGGCCT GGTGCTCTGG 
TATGACAGCG TGGTGCAAAG TGGGCAGCTC AAATGGCAAG ACGAACTCAA CCAGCACAAC 
AGGGTCTTCT TTGATTCCTG CGACGGCTTC TTCACTAACT ATAACTGGCG GGAGGAGCAC 
TTGGAGCGGA TGCTGGGGCA GGCTGGGGAG CGCCGGGCTG ATGTGTACGT GGGCGTGGAT 
GTGTTTGCTC GAGGGAACGT GGTCGGAGGC CGATTCGACA CAGACAAGTC GTTGGAGCTG 
ATCCGAAAGC ATGGCTTCTC CGTGGCTTTG TTTGCCCCCG GCTGGGTGTA TGAGTGTCTG 
GAGAAGAAGG ATTTCTTCCA GAACCAGGAC AAGTTCTGGG GCCGACTGGA GCGTTATCTG 
CCCACACATA GCATCTGCTC CTTGCCTTTC GTCACGTCCT TCTGCCTGGG CATGGGTGCA 
CGGAGGGTCT GCTATGGCCA GGAAGAGGCG GTAGGGCCCT GGTACCACCT GAGCGCCCAG 
GAGATCCAGC CCTTGTTTGG AGAACACAGG CTGGGAGGGG ATGGCCGGGG CTGGGTGAGG 
ACGCACTGCT GCCTGGAGGA TGCCTGGCAC GGAGGCAGCT CCCTGCTCGT CCGGGGTGTG 
ATCCCACCGG AGGTTGGAAA TGTGGCTGTG AGGTTATTTT CCCTGCAGGC CCCAGTGCCA 
CCCAAGATTT ACCTGTCCAT GGTGTATAAG CTTGAGGGGC CCACGGACGT CACAGTTGCT 
TTGGAGCTGA CCACAGGGGA TGCCGGCAGC TGCCACATCG GTGGCATCTC AGTGTTGAAC 
GCAGAAACAA GCTCAAGACA CAGCCTCCGA CCCCTCCGGG TGCCCCCCAC CAAGCTGGCC 
AGATGGGTGG GCCGCTGCGG CCGGCAGCTG AGTGGGGGCT GGGTCCAGCA CTGCTACGAG 
GTGAGCCTGC GTGGGTGCCT GCTGCTAGAC CTCCTCGTTT GCTTCTCACG GCCGCCGGGT 
AGTCGGGAGG AGGAGAGCTT CACCTGTCGG CTTGGAGAGA TCCAGGTGGT GGACGCTGCC 
AGCCTGCTGG CCCCTCTGCC CCAGGTGCAG GCCGTCACCA TCTCTCACAT CCGCTGGCAG 
CCATCCGCCT CTGAGCGGGA GGGGCCCCCT GCTCTGCTCC AGCTCAGCTG CACCCTGCAC 
TGGTCCTTCC TCCTCTCACA AGTCCGTTGC TTCCGAATCC ACTGCTGGGG AGGGATGAGT 
GATGACTCTC CGGGCAGGGA GCTGCCGAGG CCAGAGATGC CCATGTTCCT GGGGTTGGCT 
TTTGCCACCC AGTACCGGAT AGTGGACCTG CTGGTGGAAG CCGCCGGGCC CGGCCAGGAT 
CGTCGCATGG AATTTCTGGT GGAGCCTGTC CCCAAGGAAG GGTTCCGGGT ACCTCAGGCC 
GAGTGGGGCA GGGCAGTTCT GCTTTATTCA GCCCCTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880450.1
CDS	241..2082
Translation	MMGGYLDDRFIQGSVVQTPYAFYHWQCIDVFVYFSHHTVTIPPVGWTNTAHRHGVCVLGTFITEWNEGGRLCEAFLAGDERSYQAVADRLVQITQFFRFDGWLINIENSLSLAAVGNMPPFLRYLTTQLHRQVPGGLVLWYDSVVQSGQLKWQDELNQHNRVFFDSCDGFFTNYNWREEHLERMLGQAGERRADVYVGVDVFARGNVVGGRFDTDKSLELIRKHGFSVALFAPGWVYECLEKKDFFQNQDKFWGRLERYLPTHSICSLPFVTSFCLGMGARRVCYGQEEAVGPWYHLSAQEIQPLFGEHRLGGDGRGWVRTHCCLEDAWHGGSSLLVRGVIPPEVGNVAVRLFSLQAPVPPKIYLSMVYKLEGPTDVTVALELTTGDAGSCHIGGISVLNAETSSRHSLRPLRVPPTKLARWVGRCGRQLSGGWVQHCYEVSLRGCLLLDLLVCFSRPPGSREEESFTCRLGEIQVVDAASLLAPLPQVQAVTISHIRWQPSASEREGPPALLQLSCTLHWSFLLSQVRCFRIHCWGGMSDDSPGRELPRPEMPMFLGLAFATQYRIVDLLVEAAGPGQDRRMEFLVEPVPKEGFRVPQAEWGRAVLLYSAPA

Target ORF information:

RefSeq Version	XM_017024961.1
Organism	Homo sapiens(human)
Definition	Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024961.1

ORF Insert Sequence:

ATGATGGGCG GGTACCTGGA TGACAGGTTC ATTCAGGGCT CGGTGGTGCA GACTCCCTAT 
GCTTTCTACC ACTGGCAGTG CATCGACGTC TTTGTGTACT TCAGCCACCA CACCGTCACC 
ATTCCCCCAG TGGGCTGGAC CAACACTGCC CACAGGCATG GGGTCTGCGT GCTGGGGACT 
TTCATCACGG AGTGGAATGA AGGGGGAAGG CTCTGTGAAG CCTTCCTGGC CGGGGATGAG 
CGCTCGTACC AGGCAGTGGC TGACCGGCTG GTCCAGATCA CTCAGTTTTT TCGTTTTGAT 
GGCTGGCTGA TCAACATCGA GAACTCGCTG AGTCTGGCCG CTGTGGGGAA CATGCCTCCT 
TTCCTGCGGT ACCTCACCAC ACAGCTGCAC CGGCAGGTCC CAGGGGGCCT GGTGCTCTGG 
TATGACAGCG TGGTGCAAAG TGGGCAGCTC AAATGGCAAG ACGAACTCAA CCAGCACAAC 
AGGGTCTTCT TTGATTCCTG CGACGGCTTC TTCACTAACT ATAACTGGCG GGAGGAGCAC 
TTGGAGCGGA TGCTGGGGCA GGCTGGGGAG CGCCGGGCTG ATGTGTACGT GGGCGTGGAT 
GTGTTTGCTC GAGGGAACGT GGTCGGAGGC CGATTCGACA CAGACAAGTC GTTGGAGCTG 
ATCCGAAAGC ATGGCTTCTC CGTGGCTTTG TTTGCCCCCG GCTGGGTGTA TGAGTGTCTG 
GAGAAGAAGG ATTTCTTCCA GAACCAGGAC AAGTTCTGGG GCCGACTGGA GCGTTATCTG 
CCCACACATA GCATCTGCTC CTTGCCTTTC GTCACGTCCT TCTGCCTGGG CATGGGTGCA 
CGGAGGGTCT GCTATGGCCA GGAAGAGGCG GTAGGGCCCT GGTACCACCT GAGCGCCCAG 
GAGATCCAGC CCTTGTTTGG AGAACACAGG CTGGGAGGGG ATGGCCGGGG CTGGGTGAGG 
ACGCACTGCT GCCTGGAGGA TGCCTGGCAC GGAGGCAGCT CCCTGCTCGT CCGGGGTGTG 
ATCCCACCGG AGGTTGGAAA TGTGGCTGTG AGGTTATTTT CCCTGCAGGC CCCAGTGCCA 
CCCAAGATTT ACCTGTCCAT GGTGTATAAG CTTGAGGGGC CCACGGACGT CACAGTTGCT 
TTGGAGCTGA CCACAGGGGA TGCCGGCAGC TGCCACATCG GTGGCATCTC AGTGTTGAAC 
GCAGAAACAA GCTCAAGACA CAGCCTCCGA CCCCTCCGGG TGCCCCCCAC CAAGCTGGCC 
AGATGGGTGG GCCGCTGCGG CCGGCAGCTG AGTGGGGGCT GGGTCCAGCA CTGCTACGAG 
GTGAGCCTGC GTGGGTGCCT GCTGCTAGAC CTCCTCGTTT GCTTCTCACG GCCGCCGGGT 
AGTCGGGAGG AGGAGAGCTT CACCTGTCGG CTTGGAGAGA TCCAGGTGGT GGACGCTGCC 
AGCCTGCTGG CCCCTCTGCC CCAGGTGCAG GCCGTCACCA TCTCTCACAT CCGCTGGCAG 
CCATCCGCCT CTGAGCGGGA GGGGCCCCCT GCTCTGCTCC AGCTCAGCTG CACCCTGCAC 
TGGTCCTTCC TCCTCTCACA AGTCCGTTGC TTCCGAATCC ACTGCTGGGG AGGGATGAGT 
GATGACTCTC CGGGCAGGGA GCTGCCGAGG CCAGAGATGC CCATGTTCCT GGGGTTGGCT 
TTTGCCACCC AGTACCGGAT AGTGGACCTG CTGGTGGAAG CCGCCGGGCC CGGCCAGGAT 
CGTCGCATGG AATTTCTGGT GGAGCCTGTC CCCAAGGAAG GGTTCCGGGT ACCTCAGGCC 
GAGTGGGGCA GGGCAGTTCT GCTTTATTCA GCCCCTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENGASE ( XM_006722017.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722080.1 Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu50589
Clone ID Related Accession (Same CDS sequence)	XM_006722017.3
Accession Version	XM_006722017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cytosolic endo-beta-N-acetylglucosaminidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGGGA CTTTCATCAC GGAGTGGAAT GAAGGGGGAA GGCTCTGTGA AGCCTTCCTG 
GCCGGGGATG AGCGCTCGTA CCAGGCAGTG GCTGACCGGC TGGTCCAGAT CACTCAGTTT 
TTTCGTTTTG ATGGCTGGCT GATCAACATC GAGAACTCGC TGAGTCTGGC CGCTGTGGGG 
AACATGCCTC CTTTCCTGCG GTACCTCACC ACACAGCTGC ACCGGCAGGT CCCAGGGGGC 
CTGGTGCTCT GGTATGACAG CGTGGTGCAA AGTGGGCAGC TCAAATGGCA AGACGAACTC 
AACCAGCACA ACAGGGTCTT CTTTGATTCC TGCGACGGCT TCTTCACTAA CTATAACTGG 
CGGGAGGAGC ACTTGGAGCG GATGCTGGGG CAGGCTGGGG AGCGCCGGGC TGATGTGTAC 
GTGGGCGTGG ATGTGTTTGC TCGAGGGAAC GTGGTCGGAG GCCGATTCGA CACAGACAAG 
TCGTTGGAGC TGATCCGAAA GCATGGCTTC TCCGTGGCTT TGTTTGCCCC CGGCTGGGTG 
TATGAGTGTC TGGAGAAGAA GGATTTCTTC CAGAACCAGG ACAAGTTCTG GGGCCGACTG 
GAGCGTTATC TGCCCACACA TAGCATCTGC TCCTTGCCTT TCGTCACGTC CTTCTGCCTG 
GGCATGGGTG CACGGAGGGT CTGCTATGGC CAGGAAGAGG CGGTAGGGCC CTGGTACCAC 
CTGAGCGCCC AGGAGATCCA GCCCTTGTTT GGAGAACACA GGCTGGGAGG GGATGGCCGG 
GGCTGGGTGA GGACGCACTG CTGCCTGGAG GATGCCTGGC ACGGAGGCAG CTCCCTGCTC 
GTCCGGGGTG TGATCCCACC GGAGGTTGGA AATGTGGCTG TGAGGTTATT TTCCCTGCAG 
GCCCCAGTGC CACCCAAGAT TTACCTGTCC ATGGTGTATA AGCTTGAGGG GCCCACGGAC 
GTCACAGTTG CTTTGGAGCT GACCACAGGG GATGCCGGCA GCTGCCACAT CGGTGGCATC 
TCAGTGTTGA ACGCAGAAAC AAGCTCAAGA CACAGCCTCC GACCCCTCCG GGTGCCCCCC 
ACCAAGCTGG CCAGATGGGT GGGCCGCTGC GGCCGGCAGC TGAGTGGGGG CTGGGTCCAG 
CACTGCTACG AGGTGAGCCT GCGTGGGTGC CTGCTGCTAG ACCTCCTCGT TTGCTTCTCA 
CGGCCGCCGG GTAGTCGGGA GGAGGAGAGC TTCACCTGTC GGCTTGGAGA GATCCAGGTG 
GTGGACGCTG CCAGCCTGCT GGCCCCTCTG CCCCAGGTGC AGGCCGTCAC CATCTCTCAC 
ATCCGCTGGC AGCCATCCGC CTCTGAGCGG GAGGGGCCCC CTGCTCTGCT CCAGCTCAGC 
TGCACCCTGC ACTGGTCCTT CCTCCTCTCA CAAGTCCGTT GCTTCCGAAT CCACTGCTGG 
GGAGGGATGA GTGATGACTC TCCGGGCAGG GAGCTGCCGA GGCCAGAGAT GCCCATGTTC 
CTGGGGTTGG CTTTTGCCAC CCAGTACCGG ATAGTGGACC TGCTGGTGGA AGCCGCCGGG 
CCCGGCCAGG ATCGTCGCAT GGAATTTCTG GTGGAGCCTG TCCCCAAGGA AGGGTTCCGG 
GTACCTCAGG CCGAGTGGGG CAGGGCAGTT CTGCTTTATT CAGCCCCTGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722080.1
CDS	88..1761
Translation	MTGTFITEWNEGGRLCEAFLAGDERSYQAVADRLVQITQFFRFDGWLINIENSLSLAAVGNMPPFLRYLTTQLHRQVPGGLVLWYDSVVQSGQLKWQDELNQHNRVFFDSCDGFFTNYNWREEHLERMLGQAGERRADVYVGVDVFARGNVVGGRFDTDKSLELIRKHGFSVALFAPGWVYECLEKKDFFQNQDKFWGRLERYLPTHSICSLPFVTSFCLGMGARRVCYGQEEAVGPWYHLSAQEIQPLFGEHRLGGDGRGWVRTHCCLEDAWHGGSSLLVRGVIPPEVGNVAVRLFSLQAPVPPKIYLSMVYKLEGPTDVTVALELTTGDAGSCHIGGISVLNAETSSRHSLRPLRVPPTKLARWVGRCGRQLSGGWVQHCYEVSLRGCLLLDLLVCFSRPPGSREEESFTCRLGEIQVVDAASLLAPLPQVQAVTISHIRWQPSASEREGPPALLQLSCTLHWSFLLSQVRCFRIHCWGGMSDDSPGRELPRPEMPMFLGLAFATQYRIVDLLVEAAGPGQDRRMEFLVEPVPKEGFRVPQAEWGRAVLLYSAPA

Target ORF information:

RefSeq Version	XM_006722017.3
Organism	Homo sapiens(human)
Definition	Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722017.3

ORF Insert Sequence:

ATGACAGGGA CTTTCATCAC GGAGTGGAAT GAAGGGGGAA GGCTCTGTGA AGCCTTCCTG 
GCCGGGGATG AGCGCTCGTA CCAGGCAGTG GCTGACCGGC TGGTCCAGAT CACTCAGTTT 
TTTCGTTTTG ATGGCTGGCT GATCAACATC GAGAACTCGC TGAGTCTGGC CGCTGTGGGG 
AACATGCCTC CTTTCCTGCG GTACCTCACC ACACAGCTGC ACCGGCAGGT CCCAGGGGGC 
CTGGTGCTCT GGTATGACAG CGTGGTGCAA AGTGGGCAGC TCAAATGGCA AGACGAACTC 
AACCAGCACA ACAGGGTCTT CTTTGATTCC TGCGACGGCT TCTTCACTAA CTATAACTGG 
CGGGAGGAGC ACTTGGAGCG GATGCTGGGG CAGGCTGGGG AGCGCCGGGC TGATGTGTAC 
GTGGGCGTGG ATGTGTTTGC TCGAGGGAAC GTGGTCGGAG GCCGATTCGA CACAGACAAG 
TCGTTGGAGC TGATCCGAAA GCATGGCTTC TCCGTGGCTT TGTTTGCCCC CGGCTGGGTG 
TATGAGTGTC TGGAGAAGAA GGATTTCTTC CAGAACCAGG ACAAGTTCTG GGGCCGACTG 
GAGCGTTATC TGCCCACACA TAGCATCTGC TCCTTGCCTT TCGTCACGTC CTTCTGCCTG 
GGCATGGGTG CACGGAGGGT CTGCTATGGC CAGGAAGAGG CGGTAGGGCC CTGGTACCAC 
CTGAGCGCCC AGGAGATCCA GCCCTTGTTT GGAGAACACA GGCTGGGAGG GGATGGCCGG 
GGCTGGGTGA GGACGCACTG CTGCCTGGAG GATGCCTGGC ACGGAGGCAG CTCCCTGCTC 
GTCCGGGGTG TGATCCCACC GGAGGTTGGA AATGTGGCTG TGAGGTTATT TTCCCTGCAG 
GCCCCAGTGC CACCCAAGAT TTACCTGTCC ATGGTGTATA AGCTTGAGGG GCCCACGGAC 
GTCACAGTTG CTTTGGAGCT GACCACAGGG GATGCCGGCA GCTGCCACAT CGGTGGCATC 
TCAGTGTTGA ACGCAGAAAC AAGCTCAAGA CACAGCCTCC GACCCCTCCG GGTGCCCCCC 
ACCAAGCTGG CCAGATGGGT GGGCCGCTGC GGCCGGCAGC TGAGTGGGGG CTGGGTCCAG 
CACTGCTACG AGGTGAGCCT GCGTGGGTGC CTGCTGCTAG ACCTCCTCGT TTGCTTCTCA 
CGGCCGCCGG GTAGTCGGGA GGAGGAGAGC TTCACCTGTC GGCTTGGAGA GATCCAGGTG 
GTGGACGCTG CCAGCCTGCT GGCCCCTCTG CCCCAGGTGC AGGCCGTCAC CATCTCTCAC 
ATCCGCTGGC AGCCATCCGC CTCTGAGCGG GAGGGGCCCC CTGCTCTGCT CCAGCTCAGC 
TGCACCCTGC ACTGGTCCTT CCTCCTCTCA CAAGTCCGTT GCTTCCGAAT CCACTGCTGG 
GGAGGGATGA GTGATGACTC TCCGGGCAGG GAGCTGCCGA GGCCAGAGAT GCCCATGTTC 
CTGGGGTTGG CTTTTGCCAC CCAGTACCGG ATAGTGGACC TGCTGGTGGA AGCCGCCGGG 
CCCGGCCAGG ATCGTCGCAT GGAATTTCTG GTGGAGCCTG TCCCCAAGGA AGGGTTCCGG 
GTACCTCAGG CCGAGTGGGG CAGGGCAGTT CTGCTTTATT CAGCCCCTGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENGASE ( XM_006722018.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722081.1 Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu50590
Clone ID Related Accession (Same CDS sequence)	XM_006722018.3
Accession Version	XM_006722018.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cytosolic endo-beta-N-acetylglucosaminidase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722018.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGTG TGCCAGAGGA CAATGGGACC GCGCAGCCAC AGCCAGTTGG GGCCCAGCCC 
CTGCCCCCCT GCCCTTGCAG CAGCGCTGAC CGCCTCTCCC GCGCCCTCTT CTGGCGGGCT 
TGTGGAACGA CGGGGGTGAT GCCCAGCAGC CTCCAGTCGT TGGAGCTGAT CCGAAAGCAT 
GGCTTCTCCG TGGCTTTGTT TGCCCCCGGC TGGGTGTATG AGTGTCTGGA GAAGAAGGAT 
TTCTTCCAGA ACCAGGACAA GTTCTGGGGC CGACTGGAGC GTTATCTGCC CACACATAGC 
ATCTGCTCCT TGCCTTTCGT CACGTCCTTC TGCCTGGGCA TGGGTGCACG GAGGGTCTGC 
TATGGCCAGG AAGAGGCGGT AGGGCCCTGG TACCACCTGA GCGCCCAGGA GATCCAGCCC 
TTGTTTGGAG AACACAGGCT GGGAGGGGAT GGCCGGGGCT GGGTGAGGAC GCACTGCTGC 
CTGGAGGATG CCTGGCACGG AGGCAGCTCC CTGCTCGTCC GGGGTGTGAT CCCACCGGAG 
GTTGGAAATG TGGCTGTGAG GTTATTTTCC CTGCAGGCCC CAGTGCCACC CAAGATTTAC 
CTGTCCATGG TGTATAAGCT TGAGGGGCCC ACGGACGTCA CAGTTGCTTT GGAGCTGACC 
ACAGGGGATG CCGGCAGCTG CCACATCGGT GGCATCTCAG TGTTGAACGC AGAAACAAGC 
TCAAGACACA GCCTCCGACC CCTCCGGGTG CCCCCCACCA AGCTGGCCAG ATGGGTGGGC 
CGCTGCGGCC GGCAGCTGAG TGGGGGCTGG GTCCAGCACT GCTACGAGGT GAGCCTGCGT 
GGGTGCCTGC TGCTAGACCT CCTCGTTTGC TTCTCACGGC CGCCGGGTAG TCGGGAGGAG 
GAGAGCTTCA CCTGTCGGCT TGGAGAGATC CAGGTGGTGG ACGCTGCCAG CCTGCTGGCC 
CCTCTGCCCC AGGTGCAGGC CGTCACCATC TCTCACATCC GCTGGCAGCC ATCCGCCTCT 
GAGCGGGAGG GGCCCCCTGC TCTGCTCCAG CTCAGCTGCA CCCTGCACTG GTCCTTCCTC 
CTCTCACAAG TCCGTTGCTT CCGAATCCAC TGCTGGGGAG GGATGAGTGA TGACTCTCCG 
GGCAGGGAGC TGCCGAGGCC AGAGATGCCC ATGTTCCTGG GGTTGGCTTT TGCCACCCAG 
TACCGGATAG TGGACCTGCT GGTGGAAGCC GCCGGGCCCG GCCAGGATCG TCGCATGGAA 
TTTCTGGTGG AGCCTGTCCC CAAGGAAGGG TTCCGGGTAC CTCAGGCCGA GTGGGGCAGG 
GCAGTTCTGC TTTATTCAGC CCCTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722081.1
CDS	475..1824
Translation	MRGVPEDNGTAQPQPVGAQPLPPCPCSSADRLSRALFWRACGTTGVMPSSLQSLELIRKHGFSVALFAPGWVYECLEKKDFFQNQDKFWGRLERYLPTHSICSLPFVTSFCLGMGARRVCYGQEEAVGPWYHLSAQEIQPLFGEHRLGGDGRGWVRTHCCLEDAWHGGSSLLVRGVIPPEVGNVAVRLFSLQAPVPPKIYLSMVYKLEGPTDVTVALELTTGDAGSCHIGGISVLNAETSSRHSLRPLRVPPTKLARWVGRCGRQLSGGWVQHCYEVSLRGCLLLDLLVCFSRPPGSREEESFTCRLGEIQVVDAASLLAPLPQVQAVTISHIRWQPSASEREGPPALLQLSCTLHWSFLLSQVRCFRIHCWGGMSDDSPGRELPRPEMPMFLGLAFATQYRIVDLLVEAAGPGQDRRMEFLVEPVPKEGFRVPQAEWGRAVLLYSAPA

Target ORF information:

RefSeq Version	XM_006722018.3
Organism	Homo sapiens(human)
Definition	Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722018.3

ORF Insert Sequence:

ATGAGAGGTG TGCCAGAGGA CAATGGGACC GCGCAGCCAC AGCCAGTTGG GGCCCAGCCC 
CTGCCCCCCT GCCCTTGCAG CAGCGCTGAC CGCCTCTCCC GCGCCCTCTT CTGGCGGGCT 
TGTGGAACGA CGGGGGTGAT GCCCAGCAGC CTCCAGTCGT TGGAGCTGAT CCGAAAGCAT 
GGCTTCTCCG TGGCTTTGTT TGCCCCCGGC TGGGTGTATG AGTGTCTGGA GAAGAAGGAT 
TTCTTCCAGA ACCAGGACAA GTTCTGGGGC CGACTGGAGC GTTATCTGCC CACACATAGC 
ATCTGCTCCT TGCCTTTCGT CACGTCCTTC TGCCTGGGCA TGGGTGCACG GAGGGTCTGC 
TATGGCCAGG AAGAGGCGGT AGGGCCCTGG TACCACCTGA GCGCCCAGGA GATCCAGCCC 
TTGTTTGGAG AACACAGGCT GGGAGGGGAT GGCCGGGGCT GGGTGAGGAC GCACTGCTGC 
CTGGAGGATG CCTGGCACGG AGGCAGCTCC CTGCTCGTCC GGGGTGTGAT CCCACCGGAG 
GTTGGAAATG TGGCTGTGAG GTTATTTTCC CTGCAGGCCC CAGTGCCACC CAAGATTTAC 
CTGTCCATGG TGTATAAGCT TGAGGGGCCC ACGGACGTCA CAGTTGCTTT GGAGCTGACC 
ACAGGGGATG CCGGCAGCTG CCACATCGGT GGCATCTCAG TGTTGAACGC AGAAACAAGC 
TCAAGACACA GCCTCCGACC CCTCCGGGTG CCCCCCACCA AGCTGGCCAG ATGGGTGGGC 
CGCTGCGGCC GGCAGCTGAG TGGGGGCTGG GTCCAGCACT GCTACGAGGT GAGCCTGCGT 
GGGTGCCTGC TGCTAGACCT CCTCGTTTGC TTCTCACGGC CGCCGGGTAG TCGGGAGGAG 
GAGAGCTTCA CCTGTCGGCT TGGAGAGATC CAGGTGGTGG ACGCTGCCAG CCTGCTGGCC 
CCTCTGCCCC AGGTGCAGGC CGTCACCATC TCTCACATCC GCTGGCAGCC ATCCGCCTCT 
GAGCGGGAGG GGCCCCCTGC TCTGCTCCAG CTCAGCTGCA CCCTGCACTG GTCCTTCCTC 
CTCTCACAAG TCCGTTGCTT CCGAATCCAC TGCTGGGGAG GGATGAGTGA TGACTCTCCG 
GGCAGGGAGC TGCCGAGGCC AGAGATGCCC ATGTTCCTGG GGTTGGCTTT TGCCACCCAG 
TACCGGATAG TGGACCTGCT GGTGGAAGCC GCCGGGCCCG GCCAGGATCG TCGCATGGAA 
TTTCTGGTGG AGCCTGTCCC CAAGGAAGGG TTCCGGGTAC CTCAGGCCGA GTGGGGCAGG 
GCAGTTCTGC TTTATTCAGC CCCTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENGASE ( NM_001042573.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001036038.1 Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), mRNA.

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CloneID	OHu00159
Clone ID Related Accession (Same CDS sequence)	NM_001042573.2 , NM_001042573.3
Accession Version	NM_001042573.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	cytosolic endo-beta-N-acetylglucosaminidase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY070811.1, AK025518.1, AF512564.1 and AC073624.28. On Oct 4, 2019 this sequence version replaced NM_001042573.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF512564.1, SRR1803616.75325.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCG CGGCGGTGAC GGTCACCCGG TCGGCTACAC GGCGGCGGCG GCGGCAGCTG 
CAGGGGCTGG CGGCCCCGGA GGCGGGGACG CAGGAGGAGC AGGAGGATCA GGAGCCGCGG 
CCGCGGCGGC GGCGGCCGGG AAGGAGCATC AAAGATGAAG AAGAAGAGAC AGTCTTTCGA 
GAGGTGGTCA GTTTTTCCCC GGACCCCCTG CCAGTTAGAT ATTATGACAA GGACACCACC 
AAACCAATCA GCTTTTACTT GTCTTCGCTG GAGGAGCTCT TGGCGTGGAA GCCCCGCTTG 
GAGGATGGCT TTAATGTGGC CCTGGAGCCC CTGGCGTGTC GCCAGCCCCC TCTGAGCAGC 
CAGAGGCCCC GGACTTTGTT GTGTCATGAC ATGATGGGCG GGTACCTGGA TGACAGGTTC 
ATTCAGGGCT CGGTGGTGCA GACTCCCTAT GCTTTCTACC ACTGGCAGTG CATCGACGTC 
TTTGTGTACT TCAGCCACCA CACCGTCACC ATTCCCCCAG TGGGCTGGAC CAACACTGCC 
CACAGGCATG GGGTCTGCGT GCTGGGGACT TTCATCACGG AGTGGAATGA AGGGGGAAGG 
CTCTGTGAAG CCTTCCTGGC CGGGGATGAG CGCTCGTACC AGGCAGTGGC TGACCGGCTG 
GTCCAGATCA CTCAGTTTTT TCGTTTTGAT GGCTGGCTGA TCAACATCGA GAACTCGCTG 
AGTCTGGCCG CTGTGGGGAA CATGCCTCCT TTCCTGCGGT ACCTCACCAC ACAGCTGCAC 
CGGCAGGTCC CAGGGGGCCT GGTGCTCTGG TATGACAGCG TGGTGCAAAG TGGGCAGCTC 
AAATGGCAAG ACGAACTCAA CCAGCACAAC AGGGTCTTCT TTGATTCCTG CGACGGCTTC 
TTCACTAACT ATAACTGGCG GGAGGAGCAC TTGGAGCGGA TGCTGGGGCA GGCTGGGGAG 
CGCCGGGCTG ATGTGTACGT GGGCGTGGAT GTGTTTGCTC GAGGGAACGT GGTCGGAGGC 
CGATTCGACA CAGACAAGTC GTTGGAGCTG ATCCGAAAGC ATGGCTTCTC CGTGGCTTTG 
TTTGCCCCCG GCTGGGTGTA TGAGTGTCTG GAGAAGAAGG ATTTCTTCCA GAACCAGGAC 
AAGTTCTGGG GCCGACTGGA GCGTTATCTG CCCACACATA GCATCTGCTC CTTGCCTTTC 
GTCACGTCCT TCTGCCTGGG CATGGGTGCA CGGAGGGTCT GCTATGGCCA GGAAGAGGCG 
GTAGGGCCCT GGTACCACCT GAGCGCCCAG GAGATCCAGC CCTTGTTTGG AGAACACAGG 
CTGGGAGGGG ATGGCCGGGG CTGGGTGAGG ACGCACTGCT GCCTGGAGGA TGCCTGGCAC 
GGAGGCAGCT CCCTGCTCGT CCGGGGTGTG ATCCCACCGG AGGTTGGAAA TGTGGCTGTG 
AGGTTATTTT CCCTGCAGGC CCCAGTGCCA CCCAAGATTT ACCTGTCCAT GGTGTATAAG 
CTTGAGGGGC CCACGGACGT CACAGTTGCT TTGGAGCTGA CCACAGGGGA TGCCGGCAGC 
TGCCACATCG GTGGCATCTC AGTGTTGAAC GCAGAAACAA GCTCAAGACA CAGCCTCCGA 
CCCCTCCGGG TGCCCCCCAC CAAGCTGGCC AGATGGGTGG GCCGCTGCGG CCGGCAGCTG 
AGTGGGGGCT GGGTCCAGCA CTGCTACGAG GTGAGCCTGC GTGGGTGCCT GCTGCTAGAC 
CTCCTCGTTT GCTTCTCACG GCCGCCGGGT AGTCGGGAGG AGGAGAGCTT CACCTGTCGG 
CTTGGAGAGA TCCAGGTGGT GGACGCTGCC AGCCTGCTGG CCCCTCTGCC CCAGGTGCAG 
GCCGTCACCA TCTCTCACAT CCGCTGGCAG CCATCCGCCT CTGAGCGGGA GGGGCCCCCT 
GCTCTGCTCC AGCTCAGCTG CACCCTGCAC TGGTCCTTCC TCCTCTCACA AGTCCGTTGC 
TTCCGAATCC ACTGCTGGGG AGGGATGAGT GATGACTCTC CGGGCAGGGA GCTGCCGAGG 
CCAGAGATGC CCATGTTCCT GGGGTTGGCT TTTGCCACCC AGTACCGGAT AGTGGACCTG 
CTGGTGGAAG CCGCCGGGCC CGGCCAGGAT CGTCGCATGG AATTTCTGGT GGAGCCTGTC 
CCCAAGGAAG GGTTCCGGGT ACCTCAGGCC GAGTGGGGCA GGGCAGTTCT GCTTTATTCA 
GCCCCTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036038.1
CDS	122..2353
Translation	MEAAAVTVTRSATRRRRRQLQGLAAPEAGTQEEQEDQEPRPRRRRPGRSIKDEEEETVFREVVSFSPDPLPVRYYDKDTTKPISFYLSSLEELLAWKPRLEDGFNVALEPLACRQPPLSSQRPRTLLCHDMMGGYLDDRFIQGSVVQTPYAFYHWQCIDVFVYFSHHTVTIPPVGWTNTAHRHGVCVLGTFITEWNEGGRLCEAFLAGDERSYQAVADRLVQITQFFRFDGWLINIENSLSLAAVGNMPPFLRYLTTQLHRQVPGGLVLWYDSVVQSGQLKWQDELNQHNRVFFDSCDGFFTNYNWREEHLERMLGQAGERRADVYVGVDVFARGNVVGGRFDTDKSLELIRKHGFSVALFAPGWVYECLEKKDFFQNQDKFWGRLERYLPTHSICSLPFVTSFCLGMGARRVCYGQEEAVGPWYHLSAQEIQPLFGEHRLGGDGRGWVRTHCCLEDAWHGGSSLLVRGVIPPEVGNVAVRLFSLQAPVPPKIYLSMVYKLEGPTDVTVALELTTGDAGSCHIGGISVLNAETSSRHSLRPLRVPPTKLARWVGRCGRQLSGGWVQHCYEVSLRGCLLLDLLVCFSRPPGSREEESFTCRLGEIQVVDAASLLAPLPQVQAVTISHIRWQPSASEREGPPALLQLSCTLHWSFLLSQVRCFRIHCWGGMSDDSPGRELPRPEMPMFLGLAFATQYRIVDLLVEAAGPGQDRRMEFLVEPVPKEGFRVPQAEWGRAVLLYSAPA

Target ORF information:

RefSeq Version	NM_001042573.3
Organism	Homo sapiens(human)
Definition	Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042573.3

ORF Insert Sequence:

ATGGAGGCCG CGGCGGTGAC GGTCACCCGG TCGGCTACAC GGCGGCGGCG GCGGCAGCTG 
CAGGGGCTGG CGGCCCCGGA GGCGGGGACG CAGGAGGAGC AGGAGGATCA GGAGCCGCGG 
CCGCGGCGGC GGCGGCCGGG AAGGAGCATC AAAGATGAAG AAGAAGAGAC AGTCTTTCGA 
GAGGTGGTCA GTTTTTCCCC GGACCCCCTG CCAGTTAGAT ATTATGACAA GGACACCACC 
AAACCAATCA GCTTTTACTT GTCTTCGCTG GAGGAGCTCT TGGCGTGGAA GCCCCGCTTG 
GAGGATGGCT TTAATGTGGC CCTGGAGCCC CTGGCGTGTC GCCAGCCCCC TCTGAGCAGC 
CAGAGGCCCC GGACTTTGTT GTGTCATGAC ATGATGGGCG GGTACCTGGA TGACAGGTTC 
ATTCAGGGCT CGGTGGTGCA GACTCCCTAT GCTTTCTACC ACTGGCAGTG CATCGACGTC 
TTTGTGTACT TCAGCCACCA CACCGTCACC ATTCCCCCAG TGGGCTGGAC CAACACTGCC 
CACAGGCATG GGGTCTGCGT GCTGGGGACT TTCATCACGG AGTGGAATGA AGGGGGAAGG 
CTCTGTGAAG CCTTCCTGGC CGGGGATGAG CGCTCGTACC AGGCAGTGGC TGACCGGCTG 
GTCCAGATCA CTCAGTTTTT TCGTTTTGAT GGCTGGCTGA TCAACATCGA GAACTCGCTG 
AGTCTGGCCG CTGTGGGGAA CATGCCTCCT TTCCTGCGGT ACCTCACCAC ACAGCTGCAC 
CGGCAGGTCC CAGGGGGCCT GGTGCTCTGG TATGACAGCG TGGTGCAAAG TGGGCAGCTC 
AAATGGCAAG ACGAACTCAA CCAGCACAAC AGGGTCTTCT TTGATTCCTG CGACGGCTTC 
TTCACTAACT ATAACTGGCG GGAGGAGCAC TTGGAGCGGA TGCTGGGGCA GGCTGGGGAG 
CGCCGGGCTG ATGTGTACGT GGGCGTGGAT GTGTTTGCTC GAGGGAACGT GGTCGGAGGC 
CGATTCGACA CAGACAAGTC GTTGGAGCTG ATCCGAAAGC ATGGCTTCTC CGTGGCTTTG 
TTTGCCCCCG GCTGGGTGTA TGAGTGTCTG GAGAAGAAGG ATTTCTTCCA GAACCAGGAC 
AAGTTCTGGG GCCGACTGGA GCGTTATCTG CCCACACATA GCATCTGCTC CTTGCCTTTC 
GTCACGTCCT TCTGCCTGGG CATGGGTGCA CGGAGGGTCT GCTATGGCCA GGAAGAGGCG 
GTAGGGCCCT GGTACCACCT GAGCGCCCAG GAGATCCAGC CCTTGTTTGG AGAACACAGG 
CTGGGAGGGG ATGGCCGGGG CTGGGTGAGG ACGCACTGCT GCCTGGAGGA TGCCTGGCAC 
GGAGGCAGCT CCCTGCTCGT CCGGGGTGTG ATCCCACCGG AGGTTGGAAA TGTGGCTGTG 
AGGTTATTTT CCCTGCAGGC CCCAGTGCCA CCCAAGATTT ACCTGTCCAT GGTGTATAAG 
CTTGAGGGGC CCACGGACGT CACAGTTGCT TTGGAGCTGA CCACAGGGGA TGCCGGCAGC 
TGCCACATCG GTGGCATCTC AGTGTTGAAC GCAGAAACAA GCTCAAGACA CAGCCTCCGA 
CCCCTCCGGG TGCCCCCCAC CAAGCTGGCC AGATGGGTGG GCCGCTGCGG CCGGCAGCTG 
AGTGGGGGCT GGGTCCAGCA CTGCTACGAG GTGAGCCTGC GTGGGTGCCT GCTGCTAGAC 
CTCCTCGTTT GCTTCTCACG GCCGCCGGGT AGTCGGGAGG AGGAGAGCTT CACCTGTCGG 
CTTGGAGAGA TCCAGGTGGT GGACGCTGCC AGCCTGCTGG CCCCTCTGCC CCAGGTGCAG 
GCCGTCACCA TCTCTCACAT CCGCTGGCAG CCATCCGCCT CTGAGCGGGA GGGGCCCCCT 
GCTCTGCTCC AGCTCAGCTG CACCCTGCAC TGGTCCTTCC TCCTCTCACA AGTCCGTTGC 
TTCCGAATCC ACTGCTGGGG AGGGATGAGT GATGACTCTC CGGGCAGGGA GCTGCCGAGG 
CCAGAGATGC CCATGTTCCT GGGGTTGGCT TTTGCCACCC AGTACCGGAT AGTGGACCTG 
CTGGTGGAAG CCGCCGGGCC CGGCCAGGAT CGTCGCATGG AATTTCTGGT GGAGCCTGTC 
CCCAAGGAAG GGTTCCGGGT ACCTCAGGCC GAGTGGGGCA GGGCAGTTCT GCTTTATTCA 
GCCCCTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENGASE ( NM_001042573.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001036038.1 Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), mRNA.

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CloneID	OHu00159
Clone ID Related Accession (Same CDS sequence)	NM_001042573.2 , NM_001042573.3
Accession Version	NM_001042573.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	cytosolic endo-beta-N-acetylglucosaminidase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK025518.1, AF512564.1 and AC073624.28. On Mar 1, 2012 this sequence version replaced NM_001042573.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.72881.1, SRR1660807.227284.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCG CGGCGGTGAC GGTCACCCGG TCGGCTACAC GGCGGCGGCG GCGGCAGCTG 
CAGGGGCTGG CGGCCCCGGA GGCGGGGACG CAGGAGGAGC AGGAGGATCA GGAGCCGCGG 
CCGCGGCGGC GGCGGCCGGG AAGGAGCATC AAAGATGAAG AAGAAGAGAC AGTCTTTCGA 
GAGGTGGTCA GTTTTTCCCC GGACCCCCTG CCAGTTAGAT ATTATGACAA GGACACCACC 
AAACCAATCA GCTTTTACTT GTCTTCGCTG GAGGAGCTCT TGGCGTGGAA GCCCCGCTTG 
GAGGATGGCT TTAATGTGGC CCTGGAGCCC CTGGCGTGTC GCCAGCCCCC TCTGAGCAGC 
CAGAGGCCCC GGACTTTGTT GTGTCATGAC ATGATGGGCG GGTACCTGGA TGACAGGTTC 
ATTCAGGGCT CGGTGGTGCA GACTCCCTAT GCTTTCTACC ACTGGCAGTG CATCGACGTC 
TTTGTGTACT TCAGCCACCA CACCGTCACC ATTCCCCCAG TGGGCTGGAC CAACACTGCC 
CACAGGCATG GGGTCTGCGT GCTGGGGACT TTCATCACGG AGTGGAATGA AGGGGGAAGG 
CTCTGTGAAG CCTTCCTGGC CGGGGATGAG CGCTCGTACC AGGCAGTGGC TGACCGGCTG 
GTCCAGATCA CTCAGTTTTT TCGTTTTGAT GGCTGGCTGA TCAACATCGA GAACTCGCTG 
AGTCTGGCCG CTGTGGGGAA CATGCCTCCT TTCCTGCGGT ACCTCACCAC ACAGCTGCAC 
CGGCAGGTCC CAGGGGGCCT GGTGCTCTGG TATGACAGCG TGGTGCAAAG TGGGCAGCTC 
AAATGGCAAG ACGAACTCAA CCAGCACAAC AGGGTCTTCT TTGATTCCTG CGACGGCTTC 
TTCACTAACT ATAACTGGCG GGAGGAGCAC TTGGAGCGGA TGCTGGGGCA GGCTGGGGAG 
CGCCGGGCTG ATGTGTACGT GGGCGTGGAT GTGTTTGCTC GAGGGAACGT GGTCGGAGGC 
CGATTCGACA CAGACAAGTC GTTGGAGCTG ATCCGAAAGC ATGGCTTCTC CGTGGCTTTG 
TTTGCCCCCG GCTGGGTGTA TGAGTGTCTG GAGAAGAAGG ATTTCTTCCA GAACCAGGAC 
AAGTTCTGGG GCCGACTGGA GCGTTATCTG CCCACACATA GCATCTGCTC CTTGCCTTTC 
GTCACGTCCT TCTGCCTGGG CATGGGTGCA CGGAGGGTCT GCTATGGCCA GGAAGAGGCG 
GTAGGGCCCT GGTACCACCT GAGCGCCCAG GAGATCCAGC CCTTGTTTGG AGAACACAGG 
CTGGGAGGGG ATGGCCGGGG CTGGGTGAGG ACGCACTGCT GCCTGGAGGA TGCCTGGCAC 
GGAGGCAGCT CCCTGCTCGT CCGGGGTGTG ATCCCACCGG AGGTTGGAAA TGTGGCTGTG 
AGGTTATTTT CCCTGCAGGC CCCAGTGCCA CCCAAGATTT ACCTGTCCAT GGTGTATAAG 
CTTGAGGGGC CCACGGACGT CACAGTTGCT TTGGAGCTGA CCACAGGGGA TGCCGGCAGC 
TGCCACATCG GTGGCATCTC AGTGTTGAAC GCAGAAACAA GCTCAAGACA CAGCCTCCGA 
CCCCTCCGGG TGCCCCCCAC CAAGCTGGCC AGATGGGTGG GCCGCTGCGG CCGGCAGCTG 
AGTGGGGGCT GGGTCCAGCA CTGCTACGAG GTGAGCCTGC GTGGGTGCCT GCTGCTAGAC 
CTCCTCGTTT GCTTCTCACG GCCGCCGGGT AGTCGGGAGG AGGAGAGCTT CACCTGTCGG 
CTTGGAGAGA TCCAGGTGGT GGACGCTGCC AGCCTGCTGG CCCCTCTGCC CCAGGTGCAG 
GCCGTCACCA TCTCTCACAT CCGCTGGCAG CCATCCGCCT CTGAGCGGGA GGGGCCCCCT 
GCTCTGCTCC AGCTCAGCTG CACCCTGCAC TGGTCCTTCC TCCTCTCACA AGTCCGTTGC 
TTCCGAATCC ACTGCTGGGG AGGGATGAGT GATGACTCTC CGGGCAGGGA GCTGCCGAGG 
CCAGAGATGC CCATGTTCCT GGGGTTGGCT TTTGCCACCC AGTACCGGAT AGTGGACCTG 
CTGGTGGAAG CCGCCGGGCC CGGCCAGGAT CGTCGCATGG AATTTCTGGT GGAGCCTGTC 
CCCAAGGAAG GGTTCCGGGT ACCTCAGGCC GAGTGGGGCA GGGCAGTTCT GCTTTATTCA 
GCCCCTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036038.1
CDS	9..2240
Translation	MEAAAVTVTRSATRRRRRQLQGLAAPEAGTQEEQEDQEPRPRRRRPGRSIKDEEEETVFREVVSFSPDPLPVRYYDKDTTKPISFYLSSLEELLAWKPRLEDGFNVALEPLACRQPPLSSQRPRTLLCHDMMGGYLDDRFIQGSVVQTPYAFYHWQCIDVFVYFSHHTVTIPPVGWTNTAHRHGVCVLGTFITEWNEGGRLCEAFLAGDERSYQAVADRLVQITQFFRFDGWLINIENSLSLAAVGNMPPFLRYLTTQLHRQVPGGLVLWYDSVVQSGQLKWQDELNQHNRVFFDSCDGFFTNYNWREEHLERMLGQAGERRADVYVGVDVFARGNVVGGRFDTDKSLELIRKHGFSVALFAPGWVYECLEKKDFFQNQDKFWGRLERYLPTHSICSLPFVTSFCLGMGARRVCYGQEEAVGPWYHLSAQEIQPLFGEHRLGGDGRGWVRTHCCLEDAWHGGSSLLVRGVIPPEVGNVAVRLFSLQAPVPPKIYLSMVYKLEGPTDVTVALELTTGDAGSCHIGGISVLNAETSSRHSLRPLRVPPTKLARWVGRCGRQLSGGWVQHCYEVSLRGCLLLDLLVCFSRPPGSREEESFTCRLGEIQVVDAASLLAPLPQVQAVTISHIRWQPSASEREGPPALLQLSCTLHWSFLLSQVRCFRIHCWGGMSDDSPGRELPRPEMPMFLGLAFATQYRIVDLLVEAAGPGQDRRMEFLVEPVPKEGFRVPQAEWGRAVLLYSAPA

Target ORF information:

RefSeq Version	NM_001042573.2
Organism	Homo sapiens(human)
Definition	Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042573.2

ORF Insert Sequence:

ATGGAGGCCG CGGCGGTGAC GGTCACCCGG TCGGCTACAC GGCGGCGGCG GCGGCAGCTG 
CAGGGGCTGG CGGCCCCGGA GGCGGGGACG CAGGAGGAGC AGGAGGATCA GGAGCCGCGG 
CCGCGGCGGC GGCGGCCGGG AAGGAGCATC AAAGATGAAG AAGAAGAGAC AGTCTTTCGA 
GAGGTGGTCA GTTTTTCCCC GGACCCCCTG CCAGTTAGAT ATTATGACAA GGACACCACC 
AAACCAATCA GCTTTTACTT GTCTTCGCTG GAGGAGCTCT TGGCGTGGAA GCCCCGCTTG 
GAGGATGGCT TTAATGTGGC CCTGGAGCCC CTGGCGTGTC GCCAGCCCCC TCTGAGCAGC 
CAGAGGCCCC GGACTTTGTT GTGTCATGAC ATGATGGGCG GGTACCTGGA TGACAGGTTC 
ATTCAGGGCT CGGTGGTGCA GACTCCCTAT GCTTTCTACC ACTGGCAGTG CATCGACGTC 
TTTGTGTACT TCAGCCACCA CACCGTCACC ATTCCCCCAG TGGGCTGGAC CAACACTGCC 
CACAGGCATG GGGTCTGCGT GCTGGGGACT TTCATCACGG AGTGGAATGA AGGGGGAAGG 
CTCTGTGAAG CCTTCCTGGC CGGGGATGAG CGCTCGTACC AGGCAGTGGC TGACCGGCTG 
GTCCAGATCA CTCAGTTTTT TCGTTTTGAT GGCTGGCTGA TCAACATCGA GAACTCGCTG 
AGTCTGGCCG CTGTGGGGAA CATGCCTCCT TTCCTGCGGT ACCTCACCAC ACAGCTGCAC 
CGGCAGGTCC CAGGGGGCCT GGTGCTCTGG TATGACAGCG TGGTGCAAAG TGGGCAGCTC 
AAATGGCAAG ACGAACTCAA CCAGCACAAC AGGGTCTTCT TTGATTCCTG CGACGGCTTC 
TTCACTAACT ATAACTGGCG GGAGGAGCAC TTGGAGCGGA TGCTGGGGCA GGCTGGGGAG 
CGCCGGGCTG ATGTGTACGT GGGCGTGGAT GTGTTTGCTC GAGGGAACGT GGTCGGAGGC 
CGATTCGACA CAGACAAGTC GTTGGAGCTG ATCCGAAAGC ATGGCTTCTC CGTGGCTTTG 
TTTGCCCCCG GCTGGGTGTA TGAGTGTCTG GAGAAGAAGG ATTTCTTCCA GAACCAGGAC 
AAGTTCTGGG GCCGACTGGA GCGTTATCTG CCCACACATA GCATCTGCTC CTTGCCTTTC 
GTCACGTCCT TCTGCCTGGG CATGGGTGCA CGGAGGGTCT GCTATGGCCA GGAAGAGGCG 
GTAGGGCCCT GGTACCACCT GAGCGCCCAG GAGATCCAGC CCTTGTTTGG AGAACACAGG 
CTGGGAGGGG ATGGCCGGGG CTGGGTGAGG ACGCACTGCT GCCTGGAGGA TGCCTGGCAC 
GGAGGCAGCT CCCTGCTCGT CCGGGGTGTG ATCCCACCGG AGGTTGGAAA TGTGGCTGTG 
AGGTTATTTT CCCTGCAGGC CCCAGTGCCA CCCAAGATTT ACCTGTCCAT GGTGTATAAG 
CTTGAGGGGC CCACGGACGT CACAGTTGCT TTGGAGCTGA CCACAGGGGA TGCCGGCAGC 
TGCCACATCG GTGGCATCTC AGTGTTGAAC GCAGAAACAA GCTCAAGACA CAGCCTCCGA 
CCCCTCCGGG TGCCCCCCAC CAAGCTGGCC AGATGGGTGG GCCGCTGCGG CCGGCAGCTG 
AGTGGGGGCT GGGTCCAGCA CTGCTACGAG GTGAGCCTGC GTGGGTGCCT GCTGCTAGAC 
CTCCTCGTTT GCTTCTCACG GCCGCCGGGT AGTCGGGAGG AGGAGAGCTT CACCTGTCGG 
CTTGGAGAGA TCCAGGTGGT GGACGCTGCC AGCCTGCTGG CCCCTCTGCC CCAGGTGCAG 
GCCGTCACCA TCTCTCACAT CCGCTGGCAG CCATCCGCCT CTGAGCGGGA GGGGCCCCCT 
GCTCTGCTCC AGCTCAGCTG CACCCTGCAC TGGTCCTTCC TCCTCTCACA AGTCCGTTGC 
TTCCGAATCC ACTGCTGGGG AGGGATGAGT GATGACTCTC CGGGCAGGGA GCTGCCGAGG 
CCAGAGATGC CCATGTTCCT GGGGTTGGCT TTTGCCACCC AGTACCGGAT AGTGGACCTG 
CTGGTGGAAG CCGCCGGGCC CGGCCAGGAT CGTCGCATGG AATTTCTGGT GGAGCCTGTC 
CCCAAGGAAG GGTTCCGGGT ACCTCAGGCC GAGTGGGGCA GGGCAGTTCT GCTTTATTCA 
GCCCCTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENGASE ( XM_006722019.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722082.1 Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X11, mRNA.

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CloneID	OHu50591
Clone ID Related Accession (Same CDS sequence)	XM_006722019.3
Accession Version	XM_006722019.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cytosolic endo-beta-N-acetylglucosaminidase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722019.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCG CGGCGGTGAC GGTCACCCGG TCGGCTACAC GGCGGCGGCG GCGGCAGCTG 
CAGGGGCTGG CGGCCCCGGA GGCGGGGACG CAGGAGGAGC AGGAGGATCA GGAGCCGCGG 
CCGCGGCGGC GGCGGCCGGG AAGGAGCATC AAAGATGAAG AAGAAGAGAC AGTCTTTCGA 
GAGGTGGTCA GTTTTTCCCC GGACCCCCTG CCAGTTAGAT ATTATGACAA GGACACCACC 
AAACCAATCA GCTTTTACTT GTCTTCGCTG GAGGAGCTCT TGGCGTGGAA GCCCCGCTTG 
GAGGATGGCT TTAATGTGGC CCTGGAGCCC CTGGCGTGTC GCCAGCCCCC TCTGAGCAGC 
CAGAGGCCCC GGACTTTGTT GTGTCATGAC ATGATGGGCG GGTACCTGGA TGACAGGTTC 
ATTCAGGGCT CGGTGGTGCA GACTCCCTAT GCTTTCTACC ACTGGCAGTG CATCGACGTC 
TTTGTGTACT TCAGCCACCA CACCGTCACC ATTCCCCCAG TGGGCTGGAC CAACACTGCC 
CACAGGCATG GGGTCTGCGT GCTGGGGACT TTCATCACGG AGTGGAATGA AGGGGGAAGG 
CTCTGTGAAG CCTTCCTGGC CGGGGATGAG CGCTCGTACC AGGCAGTGGC TGACCGGCTG 
GTCCAGATCA CTCAGTTTTT TCGTTTTGAT GGCTGGCTGA TCAACATCGA GAACTCGCTG 
AGTCTGGCCG CTGTGGGGAA CATGCCTCCT TTCCTGCGGT ACCTCACCAC ACAGCTGCAC 
CGGCAGGTCC CAGGGGGCCT GGTGCTCTGG TATGACAGCG TGGTGCAAAG TGGGCAGCTC 
AAATGGCAAG ACGAACTCAA CCAGCACAAC AGGGTCTTCT TTGATTCCTG CGACGGCTTC 
TTCACTAACT ATAACTGGCG GGAGGAGCAC TTGGAGCGGA TGCTGGGGCA GGCTGGGGAG 
CGCCGGGCTG ATGTGTACGT GGGCGTGGAT GTGTTTGCTC GAGGGAACGT GGTCGGAGGC 
CGATTCGACA CAGACAAGTC GTTGGAGCTG ATCCGAAAGC ATGGCTTCTC CGTGGCTTTG 
TTTGCCCCCG GCTGGGTGTA TGAGTGTCTG GAGAAGAAGG ATTTCTTCCA GAACCAGGAC 
AAGAAGAGGC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722082.1
CDS	134..1288
Translation	MEAAAVTVTRSATRRRRRQLQGLAAPEAGTQEEQEDQEPRPRRRRPGRSIKDEEEETVFREVVSFSPDPLPVRYYDKDTTKPISFYLSSLEELLAWKPRLEDGFNVALEPLACRQPPLSSQRPRTLLCHDMMGGYLDDRFIQGSVVQTPYAFYHWQCIDVFVYFSHHTVTIPPVGWTNTAHRHGVCVLGTFITEWNEGGRLCEAFLAGDERSYQAVADRLVQITQFFRFDGWLINIENSLSLAAVGNMPPFLRYLTTQLHRQVPGGLVLWYDSVVQSGQLKWQDELNQHNRVFFDSCDGFFTNYNWREEHLERMLGQAGERRADVYVGVDVFARGNVVGGRFDTDKSLELIRKHGFSVALFAPGWVYECLEKKDFFQNQDKKRR

Target ORF information:

RefSeq Version	XM_006722019.3
Organism	Homo sapiens(human)
Definition	Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722019.3

ORF Insert Sequence:

ATGGAGGCCG CGGCGGTGAC GGTCACCCGG TCGGCTACAC GGCGGCGGCG GCGGCAGCTG 
CAGGGGCTGG CGGCCCCGGA GGCGGGGACG CAGGAGGAGC AGGAGGATCA GGAGCCGCGG 
CCGCGGCGGC GGCGGCCGGG AAGGAGCATC AAAGATGAAG AAGAAGAGAC AGTCTTTCGA 
GAGGTGGTCA GTTTTTCCCC GGACCCCCTG CCAGTTAGAT ATTATGACAA GGACACCACC 
AAACCAATCA GCTTTTACTT GTCTTCGCTG GAGGAGCTCT TGGCGTGGAA GCCCCGCTTG 
GAGGATGGCT TTAATGTGGC CCTGGAGCCC CTGGCGTGTC GCCAGCCCCC TCTGAGCAGC 
CAGAGGCCCC GGACTTTGTT GTGTCATGAC ATGATGGGCG GGTACCTGGA TGACAGGTTC 
ATTCAGGGCT CGGTGGTGCA GACTCCCTAT GCTTTCTACC ACTGGCAGTG CATCGACGTC 
TTTGTGTACT TCAGCCACCA CACCGTCACC ATTCCCCCAG TGGGCTGGAC CAACACTGCC 
CACAGGCATG GGGTCTGCGT GCTGGGGACT TTCATCACGG AGTGGAATGA AGGGGGAAGG 
CTCTGTGAAG CCTTCCTGGC CGGGGATGAG CGCTCGTACC AGGCAGTGGC TGACCGGCTG 
GTCCAGATCA CTCAGTTTTT TCGTTTTGAT GGCTGGCTGA TCAACATCGA GAACTCGCTG 
AGTCTGGCCG CTGTGGGGAA CATGCCTCCT TTCCTGCGGT ACCTCACCAC ACAGCTGCAC 
CGGCAGGTCC CAGGGGGCCT GGTGCTCTGG TATGACAGCG TGGTGCAAAG TGGGCAGCTC 
AAATGGCAAG ACGAACTCAA CCAGCACAAC AGGGTCTTCT TTGATTCCTG CGACGGCTTC 
TTCACTAACT ATAACTGGCG GGAGGAGCAC TTGGAGCGGA TGCTGGGGCA GGCTGGGGAG 
CGCCGGGCTG ATGTGTACGT GGGCGTGGAT GTGTTTGCTC GAGGGAACGT GGTCGGAGGC 
CGATTCGACA CAGACAAGTC GTTGGAGCTG ATCCGAAAGC ATGGCTTCTC CGTGGCTTTG 
TTTGCCCCCG GCTGGGTGTA TGAGTGTCTG GAGAAGAAGG ATTTCTTCCA GAACCAGGAC 
AAGAAGAGGC GGTAG