SMURF2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMURF2
Entrez Gene ID	64750
Full Name	SMAD specific E3 ubiquitin protein ligase 2
General protein information	Preferred Names SMAD specific E3 ubiquitin protein ligase 2 Names E3 ubiquitin-protein ligase SMURF2 E3 ubiquitin ligase SMURF2 HECT-type E3 ubiquitin transferase SMURF2 SMAD ubiquitination regulatory factor 2 hSMURF2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q23.3-q24.1
Disorder MIM:	605532

mRNA and Protein(s)

mRNA	Protein	Name
XM_005257585.3	XP_005257642.1	E3 ubiquitin-protein ligase SMURF2 isoform X1
NM_022739.4	NP_073576.1	E3 ubiquitin-protein ligase SMURF2
XM_011525128.1	XP_011523430.1	E3 ubiquitin-protein ligase SMURF2 isoform X1
NM_022739.3	NP_073576.1	E3 ubiquitin-protein ligase SMURF2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	lack	NP_523779.1
	SMURF2	XP_001109913.2
Bos taurus (cattle)	SMURF2	XP_002696169.3
Gallus gallus (chicken)	SMURF2	XP_425380.2
Homo sapiens (human)	SMURF2	NP_073576.1
Danio rerio (zebrafish)	smurf2	NP_001107898.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011242	XP_309403.3
Pan troglodytes (chimpanzee)	SMURF2	XP_511577.3
Canis lupus familiaris (dog)	SMURF2	XP_537589.4
Mus musculus (house mouse)	Smurf2	NP_079757.2
Rattus norvegicus (Norway rat)	Smurf2	NP_001100531.1

Gene Ontology
Bibliography

Related articles in PubMed

Smurf2, an E3 ubiquitin ligase, interacts with PDE4B and attenuates liver fibrosis through miR-132 mediated CTGF inhibition.
Cai Y, Huang G, Ma L, Dong L, Chen S, Shen X, Zhang S, Xue R, Sun D, Zhang S
Biochimica et biophysica acta1865(2)297-308(2018 Feb)

Smurf2-Mediated Stabilization of DNA Topoisomerase II? Controls Genomic Integrity.
Emanuelli A, Borroni AP, Apel-Sarid L, Shah PA, Ayyathan DM, Koganti P, Levy-Cohen G, Blank M
Cancer research77(16)4217-4227(2017 Aug)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

The PIAS3-Smurf2 sumoylation pathway suppresses breast cancer organoid invasiveness.
Chandhoke AS, Chanda A, Karve K, Deng L, Bonni S
Oncotarget8(13)21001-21014(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results identified Smurf2 as an essential regulator of Topo IIalpha, providing novel insights into its control and into the suggested tumor-suppressor functions of Smurf2.
Title: Smurf2-Mediated Stabilization of DNA Topoisomerase II? Controls Genomic Integrity.

Ectopic expression of human Smurf2 driven by the Col2a1 promoter accelerated disc degeneration in Col2a1-Smurf2 transgenic mice, and that higher levels of connective tissue growth factor protein and mRNA were present in Col2a1-Smurf2 transgenic discs, indicate that Smurf2 accelerates disc degeneration via upregulation of connective tissue growth factor.
Title: Ectopic expression of Smurf2 and acceleration of age-related intervertebral disc degeneration in a mouse model.

data suggest for the first time that the choice of binding partners for SMURF2 can sustain or repress TGFbeta signaling, and RNF11 may promote TGFbeta-induced cell migration.
Title: RNF11 sequestration of the E3 ligase SMURF2 on membranes antagonizes SMAD7 down-regulation of transforming growth factor ? signaling.

Low SMURF2 expression is associated with breast cancer organoid invasiveness.
Title: The PIAS3-Smurf2 sumoylation pathway suppresses breast cancer organoid invasiveness.

found that phospho-AIMP2 dissociated from the multi-tRNA synthetase complex and translocated to the nucleus, where it bound to Smurf2
Title: Oncogenic Mutation of AIMP2/p38 Inhibits Its Tumor-Suppressive Interaction with Smurf2.

The following SMURF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMURF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19946	NM_022739.4 Latest version!	Homo sapiens SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu50587	XM_005257585.3 Latest version!	Homo sapiens SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu71431	XM_011525128.1 Latest version!	Homo sapiens SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu19946	NM_022739.3	Homo sapiens SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMURF2 ( NM_022739.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_073576.1 Homo sapiens SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), mRNA.

Price & Availability↑

CloneID	OHu19946
Clone ID Related Accession (Same CDS sequence)	NM_022739.4 , NM_022739.3
Accession Version	NM_022739.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SMURF2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132812.9, CN370887.1, CN310238.1, CB267500.1, BP363931.1, AY014180.1, BC051283.1, BC009527.1, AK002019.1 and AC009994.10. On Nov 22, 2018 this sequence version replaced NM_022739.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY014180.1, BC093876.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262435.14/ ENSP00000262435.9 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
GCAAAAAACC TGGTGAAAAA GGATTTTTTC CGACTTCCTG ATCCATTTGC TAAGGTGGTG 
GTTGATGGAT CTGGGCAATG CCATTCTACA GATACTGTGA AGAATACGCT TGATCCAAAG 
TGGAATCAGC ATTATGACCT GTATATTGGA AAGTCTGATT CAGTTACGAT CAGTGTATGG 
AATCACAAGA AGATCCATAA GAAACAAGGT GCTGGATTTC TCGGTTGTGT TCGTCTTCTT 
TCCAATGCCA TCAACCGCCT CAAAGACACT GGTTATCAGA GGTTGGATTT ATGCAAACTC 
GGGCCAAATG ACAATGATAC AGTTAGAGGA CAGATAGTAG TAAGTCTTCA GTCCAGAGAC 
CGAATAGGCA CAGGAGGACA AGTTGTGGAC TGCAGTCGTT TATTTGATAA CGATTTACCA 
GACGGCTGGG AAGAAAGGAG AACCGCCTCT GGAAGAATCC AGTATCTAAA CCATATAACA 
AGAACTACGC AATGGGAGCG CCCAACACGA CCGGCATCCG AATATTCTAG CCCTGGCAGA 
CCTCTTAGCT GCTTTGTTGA TGAGAACACT CCAATTAGTG GAACAAATGG TGCAACATGT 
GGACAGTCTT CAGATCCCAG GCTGGCAGAG AGGAGAGTCA GGTCACAACG ACATAGAAAT 
TACATGAGCA GAACACATTT ACATACTCCT CCAGACCTAC CAGAAGGCTA TGAACAGAGG 
ACAACGCAAC AAGGCCAGGT GTATTTCTTA CATACACAGA CTGGTGTGAG CACATGGCAT 
GATCCAAGAG TGCCCAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTTGG TCCATTGCCT 
CCTGGATGGG AGATCCGTAA TACGGCAACA GGCAGAGTTT ATTTCGTTGA CCATAACAAC 
AGAACAACAC AATTTACAGA TCCTCGGCTG TCTGCTAACT TGCATTTAGT TTTAAATCGG 
CAGAACCAAT TGAAAGACCA ACAGCAACAG CAAGTGGTAT CGTTATGTCC TGATGACACA 
GAATGCCTGA CAGTCCCAAG GTACAAGCGA GACCTGGTTC AGAAACTAAA AATTTTGCGG 
CAAGAACTTT CCCAACAACA GCCTCAGGCA GGTCATTGCC GCATTGAGGT TTCCAGGGAA 
GAGATTTTTG AGGAATCATA TCGACAGGTC ATGAAAATGA GACCAAAAGA TCTCTGGAAG 
CGATTAATGA TAAAATTTCG TGGAGAAGAA GGCCTTGACT ATGGAGGCGT TGCCAGGGAA 
TGGTTGTATC TCTTGTCACA TGAAATGTTG AATCCATACT ATGGCCTCTT CCAGTATTCA 
AGAGATGATA TTTATACATT GCAGATCAAT CCTGATTCTG CAGTTAATCC GGAACATTTA 
TCCTATTTCC ACTTTGTTGG ACGAATAATG GGAATGGCTG TGTTTCATGG ACATTATATT 
GATGGTGGTT TCACATTGCC TTTTTATAAG CAATTGCTTG GGAAGTCAAT TACCTTGGAT 
GACATGGAGT TAGTAGATCC GGATCTTCAC AACAGTTTAG TGTGGATACT TGAGAATGAT 
ATTACAGGTG TTTTGGACCA TACCTTCTGT GTTGAACATA ATGCATATGG TGAAATTATT 
CAGCATGAAC TTAAACCAAA TGGCAAAAGT ATCCCTGTTA ATGAAGAAAA TAAAAAAGAA 
TATGTCAGGC TCTATGTGAA CTGGAGATTT TTACGAGGCA TTGAGGCTCA ATTCTTGGCT 
CTGCAGAAAG GATTTAATGA AGTAATTCCA CAACATCTGC TGAAGACATT TGATGAGAAG 
GAGTTAGAGC TCATTATTTG TGGACTTGGA AAGATAGATG TTAATGACTG GAAGGTAAAC 
ACCCGGTTAA AACACTGTAC ACCAGACAGC AACATTGTCA AATGGTTCTG GAAAGCTGTG 
GAGTTTTTTG ATGAAGAGCG ACGAGCAAGA TTGCTTCAGT TTGTGACAGG ATCCTCTCGA 
GTGCCTCTGC AGGGCTTCAA AGCATTGCAA GGTGCTGCAG GCCCGAGACT CTTTACCATA 
CACCAGATTG ATGCCTGCAC TAACAACCTG CCGAAAGCCC ACACTTGCTT CAATCGAATA 
GACATTCCAC CCTATGAAAG CTATGAAAAG CTATATGAAA AGCTGCTAAC AGCCATTGAA 
GAAACATGTG GATTTGCTGT GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073576.1
CDS	428..2674
Translation	MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

Target ORF information:

RefSeq Version	NM_022739.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022739.4

ORF Insert Sequence:

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
GCAAAAAACC TGGTGAAAAA GGATTTTTTC CGACTTCCTG ATCCATTTGC TAAGGTGGTG 
GTTGATGGAT CTGGGCAATG CCATTCTACA GATACTGTGA AGAATACGCT TGATCCAAAG 
TGGAATCAGC ATTATGACCT GTATATTGGA AAGTCTGATT CAGTTACGAT CAGTGTATGG 
AATCACAAGA AGATCCATAA GAAACAAGGT GCTGGATTTC TCGGTTGTGT TCGTCTTCTT 
TCCAATGCCA TCAACCGCCT CAAAGACACT GGTTATCAGA GGTTGGATTT ATGCAAACTC 
GGGCCAAATG ACAATGATAC AGTTAGAGGA CAGATAGTAG TAAGTCTTCA GTCCAGAGAC 
CGAATAGGCA CAGGAGGACA AGTTGTGGAC TGCAGTCGTT TATTTGATAA CGATTTACCA 
GACGGCTGGG AAGAAAGGAG AACCGCCTCT GGAAGAATCC AGTATCTAAA CCATATAACA 
AGAACTACGC AATGGGAGCG CCCAACACGA CCGGCATCCG AATATTCTAG CCCTGGCAGA 
CCTCTTAGCT GCTTTGTTGA TGAGAACACT CCAATTAGTG GAACAAATGG TGCAACATGT 
GGACAGTCTT CAGATCCCAG GCTGGCAGAG AGGAGAGTCA GGTCACAACG ACATAGAAAT 
TACATGAGCA GAACACATTT ACATACTCCT CCAGACCTAC CAGAAGGCTA TGAACAGAGG 
ACAACGCAAC AAGGCCAGGT GTATTTCTTA CATACACAGA CTGGTGTGAG CACATGGCAT 
GATCCAAGAG TGCCCAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTTGG TCCATTGCCT 
CCTGGATGGG AGATCCGTAA TACGGCAACA GGCAGAGTTT ATTTCGTTGA CCATAACAAC 
AGAACAACAC AATTTACAGA TCCTCGGCTG TCTGCTAACT TGCATTTAGT TTTAAATCGG 
CAGAACCAAT TGAAAGACCA ACAGCAACAG CAAGTGGTAT CGTTATGTCC TGATGACACA 
GAATGCCTGA CAGTCCCAAG GTACAAGCGA GACCTGGTTC AGAAACTAAA AATTTTGCGG 
CAAGAACTTT CCCAACAACA GCCTCAGGCA GGTCATTGCC GCATTGAGGT TTCCAGGGAA 
GAGATTTTTG AGGAATCATA TCGACAGGTC ATGAAAATGA GACCAAAAGA TCTCTGGAAG 
CGATTAATGA TAAAATTTCG TGGAGAAGAA GGCCTTGACT ATGGAGGCGT TGCCAGGGAA 
TGGTTGTATC TCTTGTCACA TGAAATGTTG AATCCATACT ATGGCCTCTT CCAGTATTCA 
AGAGATGATA TTTATACATT GCAGATCAAT CCTGATTCTG CAGTTAATCC GGAACATTTA 
TCCTATTTCC ACTTTGTTGG ACGAATAATG GGAATGGCTG TGTTTCATGG ACATTATATT 
GATGGTGGTT TCACATTGCC TTTTTATAAG CAATTGCTTG GGAAGTCAAT TACCTTGGAT 
GACATGGAGT TAGTAGATCC GGATCTTCAC AACAGTTTAG TGTGGATACT TGAGAATGAT 
ATTACAGGTG TTTTGGACCA TACCTTCTGT GTTGAACATA ATGCATATGG TGAAATTATT 
CAGCATGAAC TTAAACCAAA TGGCAAAAGT ATCCCTGTTA ATGAAGAAAA TAAAAAAGAA 
TATGTCAGGC TCTATGTGAA CTGGAGATTT TTACGAGGCA TTGAGGCTCA ATTCTTGGCT 
CTGCAGAAAG GATTTAATGA AGTAATTCCA CAACATCTGC TGAAGACATT TGATGAGAAG 
GAGTTAGAGC TCATTATTTG TGGACTTGGA AAGATAGATG TTAATGACTG GAAGGTAAAC 
ACCCGGTTAA AACACTGTAC ACCAGACAGC AACATTGTCA AATGGTTCTG GAAAGCTGTG 
GAGTTTTTTG ATGAAGAGCG ACGAGCAAGA TTGCTTCAGT TTGTGACAGG ATCCTCTCGA 
GTGCCTCTGC AGGGCTTCAA AGCATTGCAA GGTGCTGCAG GCCCGAGACT CTTTACCATA 
CACCAGATTG ATGCCTGCAC TAACAACCTG CCGAAAGCCC ACACTTGCTT CAATCGAATA 
GACATTCCAC CCTATGAAAG CTATGAAAAG CTATATGAAA AGCTGCTAAC AGCCATTGAA 
GAAACATGTG GATTTGCTGT GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF2 ( NM_022739.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_073576.1 Homo sapiens SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), mRNA.

Price & Availability↑

CloneID	OHu19946
Clone ID Related Accession (Same CDS sequence)	NM_022739.4 , NM_022739.3
Accession Version	NM_022739.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SMURF2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN370887.1, CN310238.1, CB267500.1, BP363931.1, AY014180.1, BC051283.1, BC009527.1 and AK002019.1. On Dec 10, 2004 this sequence version replaced NM_022739.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY014180.1, BC093876.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
GCAAAAAACC TGGTGAAAAA GGATTTTTTC CGACTTCCTG ATCCATTTGC TAAGGTGGTG 
GTTGATGGAT CTGGGCAATG CCATTCTACA GATACTGTGA AGAATACGCT TGATCCAAAG 
TGGAATCAGC ATTATGACCT GTATATTGGA AAGTCTGATT CAGTTACGAT CAGTGTATGG 
AATCACAAGA AGATCCATAA GAAACAAGGT GCTGGATTTC TCGGTTGTGT TCGTCTTCTT 
TCCAATGCCA TCAACCGCCT CAAAGACACT GGTTATCAGA GGTTGGATTT ATGCAAACTC 
GGGCCAAATG ACAATGATAC AGTTAGAGGA CAGATAGTAG TAAGTCTTCA GTCCAGAGAC 
CGAATAGGCA CAGGAGGACA AGTTGTGGAC TGCAGTCGTT TATTTGATAA CGATTTACCA 
GACGGCTGGG AAGAAAGGAG AACCGCCTCT GGAAGAATCC AGTATCTAAA CCATATAACA 
AGAACTACGC AATGGGAGCG CCCAACACGA CCGGCATCCG AATATTCTAG CCCTGGCAGA 
CCTCTTAGCT GCTTTGTTGA TGAGAACACT CCAATTAGTG GAACAAATGG TGCAACATGT 
GGACAGTCTT CAGATCCCAG GCTGGCAGAG AGGAGAGTCA GGTCACAACG ACATAGAAAT 
TACATGAGCA GAACACATTT ACATACTCCT CCAGACCTAC CAGAAGGCTA TGAACAGAGG 
ACAACGCAAC AAGGCCAGGT GTATTTCTTA CATACACAGA CTGGTGTGAG CACATGGCAT 
GATCCAAGAG TGCCCAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTTGG TCCATTGCCT 
CCTGGATGGG AGATCCGTAA TACGGCAACA GGCAGAGTTT ATTTCGTTGA CCATAACAAC 
AGAACAACAC AATTTACAGA TCCTCGGCTG TCTGCTAACT TGCATTTAGT TTTAAATCGG 
CAGAACCAAT TGAAAGACCA ACAGCAACAG CAAGTGGTAT CGTTATGTCC TGATGACACA 
GAATGCCTGA CAGTCCCAAG GTACAAGCGA GACCTGGTTC AGAAACTAAA AATTTTGCGG 
CAAGAACTTT CCCAACAACA GCCTCAGGCA GGTCATTGCC GCATTGAGGT TTCCAGGGAA 
GAGATTTTTG AGGAATCATA TCGACAGGTC ATGAAAATGA GACCAAAAGA TCTCTGGAAG 
CGATTAATGA TAAAATTTCG TGGAGAAGAA GGCCTTGACT ATGGAGGCGT TGCCAGGGAA 
TGGTTGTATC TCTTGTCACA TGAAATGTTG AATCCATACT ATGGCCTCTT CCAGTATTCA 
AGAGATGATA TTTATACATT GCAGATCAAT CCTGATTCTG CAGTTAATCC GGAACATTTA 
TCCTATTTCC ACTTTGTTGG ACGAATAATG GGAATGGCTG TGTTTCATGG ACATTATATT 
GATGGTGGTT TCACATTGCC TTTTTATAAG CAATTGCTTG GGAAGTCAAT TACCTTGGAT 
GACATGGAGT TAGTAGATCC GGATCTTCAC AACAGTTTAG TGTGGATACT TGAGAATGAT 
ATTACAGGTG TTTTGGACCA TACCTTCTGT GTTGAACATA ATGCATATGG TGAAATTATT 
CAGCATGAAC TTAAACCAAA TGGCAAAAGT ATCCCTGTTA ATGAAGAAAA TAAAAAAGAA 
TATGTCAGGC TCTATGTGAA CTGGAGATTT TTACGAGGCA TTGAGGCTCA ATTCTTGGCT 
CTGCAGAAAG GATTTAATGA AGTAATTCCA CAACATCTGC TGAAGACATT TGATGAGAAG 
GAGTTAGAGC TCATTATTTG TGGACTTGGA AAGATAGATG TTAATGACTG GAAGGTAAAC 
ACCCGGTTAA AACACTGTAC ACCAGACAGC AACATTGTCA AATGGTTCTG GAAAGCTGTG 
GAGTTTTTTG ATGAAGAGCG ACGAGCAAGA TTGCTTCAGT TTGTGACAGG ATCCTCTCGA 
GTGCCTCTGC AGGGCTTCAA AGCATTGCAA GGTGCTGCAG GCCCGAGACT CTTTACCATA 
CACCAGATTG ATGCCTGCAC TAACAACCTG CCGAAAGCCC ACACTTGCTT CAATCGAATA 
GACATTCCAC CCTATGAAAG CTATGAAAAG CTATATGAAA AGCTGCTAAC AGCCATTGAA 
GAAACATGTG GATTTGCTGT GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073576.1
CDS	389..2635
Translation	MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

Target ORF information:

RefSeq Version	NM_022739.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022739.3

ORF Insert Sequence:

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
GCAAAAAACC TGGTGAAAAA GGATTTTTTC CGACTTCCTG ATCCATTTGC TAAGGTGGTG 
GTTGATGGAT CTGGGCAATG CCATTCTACA GATACTGTGA AGAATACGCT TGATCCAAAG 
TGGAATCAGC ATTATGACCT GTATATTGGA AAGTCTGATT CAGTTACGAT CAGTGTATGG 
AATCACAAGA AGATCCATAA GAAACAAGGT GCTGGATTTC TCGGTTGTGT TCGTCTTCTT 
TCCAATGCCA TCAACCGCCT CAAAGACACT GGTTATCAGA GGTTGGATTT ATGCAAACTC 
GGGCCAAATG ACAATGATAC AGTTAGAGGA CAGATAGTAG TAAGTCTTCA GTCCAGAGAC 
CGAATAGGCA CAGGAGGACA AGTTGTGGAC TGCAGTCGTT TATTTGATAA CGATTTACCA 
GACGGCTGGG AAGAAAGGAG AACCGCCTCT GGAAGAATCC AGTATCTAAA CCATATAACA 
AGAACTACGC AATGGGAGCG CCCAACACGA CCGGCATCCG AATATTCTAG CCCTGGCAGA 
CCTCTTAGCT GCTTTGTTGA TGAGAACACT CCAATTAGTG GAACAAATGG TGCAACATGT 
GGACAGTCTT CAGATCCCAG GCTGGCAGAG AGGAGAGTCA GGTCACAACG ACATAGAAAT 
TACATGAGCA GAACACATTT ACATACTCCT CCAGACCTAC CAGAAGGCTA TGAACAGAGG 
ACAACGCAAC AAGGCCAGGT GTATTTCTTA CATACACAGA CTGGTGTGAG CACATGGCAT 
GATCCAAGAG TGCCCAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTTGG TCCATTGCCT 
CCTGGATGGG AGATCCGTAA TACGGCAACA GGCAGAGTTT ATTTCGTTGA CCATAACAAC 
AGAACAACAC AATTTACAGA TCCTCGGCTG TCTGCTAACT TGCATTTAGT TTTAAATCGG 
CAGAACCAAT TGAAAGACCA ACAGCAACAG CAAGTGGTAT CGTTATGTCC TGATGACACA 
GAATGCCTGA CAGTCCCAAG GTACAAGCGA GACCTGGTTC AGAAACTAAA AATTTTGCGG 
CAAGAACTTT CCCAACAACA GCCTCAGGCA GGTCATTGCC GCATTGAGGT TTCCAGGGAA 
GAGATTTTTG AGGAATCATA TCGACAGGTC ATGAAAATGA GACCAAAAGA TCTCTGGAAG 
CGATTAATGA TAAAATTTCG TGGAGAAGAA GGCCTTGACT ATGGAGGCGT TGCCAGGGAA 
TGGTTGTATC TCTTGTCACA TGAAATGTTG AATCCATACT ATGGCCTCTT CCAGTATTCA 
AGAGATGATA TTTATACATT GCAGATCAAT CCTGATTCTG CAGTTAATCC GGAACATTTA 
TCCTATTTCC ACTTTGTTGG ACGAATAATG GGAATGGCTG TGTTTCATGG ACATTATATT 
GATGGTGGTT TCACATTGCC TTTTTATAAG CAATTGCTTG GGAAGTCAAT TACCTTGGAT 
GACATGGAGT TAGTAGATCC GGATCTTCAC AACAGTTTAG TGTGGATACT TGAGAATGAT 
ATTACAGGTG TTTTGGACCA TACCTTCTGT GTTGAACATA ATGCATATGG TGAAATTATT 
CAGCATGAAC TTAAACCAAA TGGCAAAAGT ATCCCTGTTA ATGAAGAAAA TAAAAAAGAA 
TATGTCAGGC TCTATGTGAA CTGGAGATTT TTACGAGGCA TTGAGGCTCA ATTCTTGGCT 
CTGCAGAAAG GATTTAATGA AGTAATTCCA CAACATCTGC TGAAGACATT TGATGAGAAG 
GAGTTAGAGC TCATTATTTG TGGACTTGGA AAGATAGATG TTAATGACTG GAAGGTAAAC 
ACCCGGTTAA AACACTGTAC ACCAGACAGC AACATTGTCA AATGGTTCTG GAAAGCTGTG 
GAGTTTTTTG ATGAAGAGCG ACGAGCAAGA TTGCTTCAGT TTGTGACAGG ATCCTCTCGA 
GTGCCTCTGC AGGGCTTCAA AGCATTGCAA GGTGCTGCAG GCCCGAGACT CTTTACCATA 
CACCAGATTG ATGCCTGCAC TAACAACCTG CCGAAAGCCC ACACTTGCTT CAATCGAATA 
GACATTCCAC CCTATGAAAG CTATGAAAAG CTATATGAAA AGCTGCTAAC AGCCATTGAA 
GAAACATGTG GATTTGCTGT GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF2 ( XM_005257585.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005257642.1 Homo sapiens SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu50587
Clone ID Related Accession (Same CDS sequence)	XM_005257585.3
Accession Version	XM_005257585.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SMURF2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257585.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGGACTTCCT 
GATCCATTTG CTAAGGTGGT GGTTGATGGA TCTGGGCAAT GCCATTCTAC AGATACTGTG 
AAGAATACGC TTGATCCAAA GTGGAATCAG CATTATGACC TGTATATTGG AAAGTCTGAT 
TCAGTTACGA TCAGTGTATG GAATCACAAG AAGATCCATA AGAAACAAGG TGCTGGATTT 
CTCGGTTGTG TTCGTCTTCT TTCCAATGCC ATCAACCGCC TCAAAGACAC TGGTTATCAG 
AGGTTGGATT TATGCAAACT CGGGCCAAAT GACAATGATA CAGTTAGAGG ACAGATAGTA 
GTAAGTCTTC AGTCCAGAGA CCGAATAGGC ACAGGAGGAC AAGTTGTGGA CTGCAGTCGT 
TTATTTGATA ACGATTTACC AGACGGCTGG GAAGAAAGGA GAACCGCCTC TGGAAGAATC 
CAGTATCTAA ACCATATAAC AAGAACTACG CAATGGGAGC GCCCAACACG ACCGGCATCC 
GAATATTCTA GCCCTGGCAG ACCTCTTAGC TGCTTTGTTG ATGAGAACAC TCCAATTAGT 
GGAACAAATG GTGCAACATG TGGACAGTCT TCAGATCCCA GGCTGGCAGA GAGGAGAGTC 
AGGTCACAAC GACATAGAAA TTACATGAGC AGAACACATT TACATACTCC TCCAGACCTA 
CCAGAAGGCT ATGAACAGAG GACAACGCAA CAAGGCCAGG TGTATTTCTT ACATACACAG 
ACTGGTGTGA GCACATGGCA TGATCCAAGA GTGCCCAGGG ATCTTAGCAA CATCAATTGT 
GAAGAGCTTG GTCCATTGCC TCCTGGATGG GAGATCCGTA ATACGGCAAC AGGCAGAGTT 
TATTTCGTTG ACCATAACAA CAGAACAACA CAATTTACAG ATCCTCGGCT GTCTGCTAAC 
TTGCATTTAG TTTTAAATCG GCAGAACCAA TTGAAAGACC AACAGCAACA GCAAGTGGTA 
TCGTTATGTC CTGATGACAC AGAATGCCTG ACAGTCCCAA GGTACAAGCG AGACCTGGTT 
CAGAAACTAA AAATTTTGCG GCAAGAACTT TCCCAACAAC AGCCTCAGGC AGGTCATTGC 
CGCATTGAGG TTTCCAGGGA AGAGATTTTT GAGGAATCAT ATCGACAGGT CATGAAAATG 
AGACCAAAAG ATCTCTGGAA GCGATTAATG ATAAAATTTC GTGGAGAAGA AGGCCTTGAC 
TATGGAGGCG TTGCCAGGGA ATGGTTGTAT CTCTTGTCAC ATGAAATGTT GAATCCATAC 
TATGGCCTCT TCCAGTATTC AAGAGATGAT ATTTATACAT TGCAGATCAA TCCTGATTCT 
GCAGTTAATC CGGAACATTT ATCCTATTTC CACTTTGTTG GACGAATAAT GGGAATGGCT 
GTGTTTCATG GACATTATAT TGATGGTGGT TTCACATTGC CTTTTTATAA GCAATTGCTT 
GGGAAGTCAA TTACCTTGGA TGACATGGAG TTAGTAGATC CGGATCTTCA CAACAGTTTA 
GTGTGGATAC TTGAGAATGA TATTACAGGT GTTTTGGACC ATACCTTCTG TGTTGAACAT 
AATGCATATG GTGAAATTAT TCAGCATGAA CTTAAACCAA ATGGCAAAAG TATCCCTGTT 
AATGAAGAAA ATAAAAAAGA ATATGTCAGG CTCTATGTGA ACTGGAGATT TTTACGAGGC 
ATTGAGGCTC AATTCTTGGC TCTGCAGAAA GGATTTAATG AAGTAATTCC ACAACATCTG 
CTGAAGACAT TTGATGAGAA GGAGTTAGAG CTCATTATTT GTGGACTTGG AAAGATAGAT 
GTTAATGACT GGAAGGTAAA CACCCGGTTA AAACACTGTA CACCAGACAG CAACATTGTC 
AAATGGTTCT GGAAAGCTGT GGAGTTTTTT GATGAAGAGC GACGAGCAAG ATTGCTTCAG 
TTTGTGACAG GATCCTCTCG AGTGCCTCTG CAGGGCTTCA AAGCATTGCA AGGTGCTGCA 
GGCCCGAGAC TCTTTACCAT ACACCAGATT GATGCCTGCA CTAACAACCT GCCGAAAGCC 
CACACTTGCT TCAATCGAAT AGACATTCCA CCCTATGAAA GCTATGAAAA GCTATATGAA 
AAGCTGCTAA CAGCCATTGA AGAAACATGT GGATTTGCTG TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005257642.1
CDS	28..2235
Translation	MSNPGGRRNGPVKLRLTGLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

Target ORF information:

RefSeq Version	XM_005257585.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257585.3

ORF Insert Sequence:

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGGACTTCCT 
GATCCATTTG CTAAGGTGGT GGTTGATGGA TCTGGGCAAT GCCATTCTAC AGATACTGTG 
AAGAATACGC TTGATCCAAA GTGGAATCAG CATTATGACC TGTATATTGG AAAGTCTGAT 
TCAGTTACGA TCAGTGTATG GAATCACAAG AAGATCCATA AGAAACAAGG TGCTGGATTT 
CTCGGTTGTG TTCGTCTTCT TTCCAATGCC ATCAACCGCC TCAAAGACAC TGGTTATCAG 
AGGTTGGATT TATGCAAACT CGGGCCAAAT GACAATGATA CAGTTAGAGG ACAGATAGTA 
GTAAGTCTTC AGTCCAGAGA CCGAATAGGC ACAGGAGGAC AAGTTGTGGA CTGCAGTCGT 
TTATTTGATA ACGATTTACC AGACGGCTGG GAAGAAAGGA GAACCGCCTC TGGAAGAATC 
CAGTATCTAA ACCATATAAC AAGAACTACG CAATGGGAGC GCCCAACACG ACCGGCATCC 
GAATATTCTA GCCCTGGCAG ACCTCTTAGC TGCTTTGTTG ATGAGAACAC TCCAATTAGT 
GGAACAAATG GTGCAACATG TGGACAGTCT TCAGATCCCA GGCTGGCAGA GAGGAGAGTC 
AGGTCACAAC GACATAGAAA TTACATGAGC AGAACACATT TACATACTCC TCCAGACCTA 
CCAGAAGGCT ATGAACAGAG GACAACGCAA CAAGGCCAGG TGTATTTCTT ACATACACAG 
ACTGGTGTGA GCACATGGCA TGATCCAAGA GTGCCCAGGG ATCTTAGCAA CATCAATTGT 
GAAGAGCTTG GTCCATTGCC TCCTGGATGG GAGATCCGTA ATACGGCAAC AGGCAGAGTT 
TATTTCGTTG ACCATAACAA CAGAACAACA CAATTTACAG ATCCTCGGCT GTCTGCTAAC 
TTGCATTTAG TTTTAAATCG GCAGAACCAA TTGAAAGACC AACAGCAACA GCAAGTGGTA 
TCGTTATGTC CTGATGACAC AGAATGCCTG ACAGTCCCAA GGTACAAGCG AGACCTGGTT 
CAGAAACTAA AAATTTTGCG GCAAGAACTT TCCCAACAAC AGCCTCAGGC AGGTCATTGC 
CGCATTGAGG TTTCCAGGGA AGAGATTTTT GAGGAATCAT ATCGACAGGT CATGAAAATG 
AGACCAAAAG ATCTCTGGAA GCGATTAATG ATAAAATTTC GTGGAGAAGA AGGCCTTGAC 
TATGGAGGCG TTGCCAGGGA ATGGTTGTAT CTCTTGTCAC ATGAAATGTT GAATCCATAC 
TATGGCCTCT TCCAGTATTC AAGAGATGAT ATTTATACAT TGCAGATCAA TCCTGATTCT 
GCAGTTAATC CGGAACATTT ATCCTATTTC CACTTTGTTG GACGAATAAT GGGAATGGCT 
GTGTTTCATG GACATTATAT TGATGGTGGT TTCACATTGC CTTTTTATAA GCAATTGCTT 
GGGAAGTCAA TTACCTTGGA TGACATGGAG TTAGTAGATC CGGATCTTCA CAACAGTTTA 
GTGTGGATAC TTGAGAATGA TATTACAGGT GTTTTGGACC ATACCTTCTG TGTTGAACAT 
AATGCATATG GTGAAATTAT TCAGCATGAA CTTAAACCAA ATGGCAAAAG TATCCCTGTT 
AATGAAGAAA ATAAAAAAGA ATATGTCAGG CTCTATGTGA ACTGGAGATT TTTACGAGGC 
ATTGAGGCTC AATTCTTGGC TCTGCAGAAA GGATTTAATG AAGTAATTCC ACAACATCTG 
CTGAAGACAT TTGATGAGAA GGAGTTAGAG CTCATTATTT GTGGACTTGG AAAGATAGAT 
GTTAATGACT GGAAGGTAAA CACCCGGTTA AAACACTGTA CACCAGACAG CAACATTGTC 
AAATGGTTCT GGAAAGCTGT GGAGTTTTTT GATGAAGAGC GACGAGCAAG ATTGCTTCAG 
TTTGTGACAG GATCCTCTCG AGTGCCTCTG CAGGGCTTCA AAGCATTGCA AGGTGCTGCA 
GGCCCGAGAC TCTTTACCAT ACACCAGATT GATGCCTGCA CTAACAACCT GCCGAAAGCC 
CACACTTGCT TCAATCGAAT AGACATTCCA CCCTATGAAA GCTATGAAAA GCTATATGAA 
AAGCTGCTAA CAGCCATTGA AGAAACATGT GGATTTGCTG TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF2 ( XM_011525128.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523430.1 Homo sapiens SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu71431
Clone ID Related Accession (Same CDS sequence)	XM_011525128.1
Accession Version	XM_011525128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SMURF2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTGT CTAGTTGCAG GAAAACAAGC TCAGGGCTCC CACTGATTCT ACATTATGTA 
CTCTGTGCAA AAAACCTGGT GAAAAAGGAT TTTTTCCGAC TTCCTGATCC ATTTGCTAAG 
GTGGTGGTTG ATGGATCTGG GCAATGCCAT TCTACAGATA CTGTGAAGAA TACGCTTGAT 
CCAAAGTGGA ATCAGCATTA TGACCTGTAT ATTGGAAAGT CTGATTCAGT TACGATCAGT 
GTATGGAATC ACAAGAAGAT CCATAAGAAA CAAGGTGCTG GATTTCTCGG TTGTGTTCGT 
CTTCTTTCCA ATGCCATCAA CCGCCTCAAA GACACTGGTT ATCAGAGGTT GGATTTATGC 
AAACTCGGGC CAAATGACAA TGATACAGTT AGAGGACAGA TAGTAGTAAG TCTTCAGTCC 
AGAGACCGAA TAGGCACAGG AGGACAAGTT GTGGACTGCA GTCGTTTATT TGATAACGAT 
TTACCAGACG GCTGGGAAGA AAGGAGAACC GCCTCTGGAA GAATCCAGTA TCTAAACCAT 
ATAACAAGAA CTACGCAATG GGAGCGCCCA ACACGACCGG CATCCGAATA TTCTAGCCCT 
GGCAGACCTC TTAGCTGCTT TGTTGATGAG AACACTCCAA TTAGTGGAAC AAATGGTGCA 
ACATGTGGAC AGTCTTCAGA TCCCAGGCTG GCAGAGAGGA GAGTCAGGTC ACAACGACAT 
AGAAATTACA TGAGCAGAAC ACATTTACAT ACTCCTCCAG ACCTACCAGA AGGCTATGAA 
CAGAGGACAA CGCAACAAGG CCAGGTGTAT TTCTTACATA CACAGACTGG TGTGAGCACA 
TGGCATGATC CAAGAGTGCC CAGGGATCTT AGCAACATCA ATTGTGAAGA GCTTGGTCCA 
TTGCCTCCTG GATGGGAGAT CCGTAATACG GCAACAGGCA GAGTTTATTT CGTTGACCAT 
AACAACAGAA CAACACAATT TACAGATCCT CGGCTGTCTG CTAACTTGCA TTTAGTTTTA 
AATCGGCAGA ACCAATTGAA AGACCAACAG CAACAGCAAG TGGTATCGTT ATGTCCTGAT 
GACACAGAAT GCCTGACAGT CCCAAGGTAC AAGCGAGACC TGGTTCAGAA ACTAAAAATT 
TTGCGGCAAG AACTTTCCCA ACAACAGCCT CAGGCAGGTC ATTGCCGCAT TGAGGTTTCC 
AGGGAAGAGA TTTTTGAGGA ATCATATCGA CAGGTCATGA AAATGAGACC AAAAGATCTC 
TGGAAGCGAT TAATGATAAA ATTTCGTGGA GAAGAAGGCC TTGACTATGG AGGCGTTGCC 
AGGGAATGGT TGTATCTCTT GTCACATGAA ATGTTGAATC CATACTATGG CCTCTTCCAG 
TATTCAAGAG ATGATATTTA TACATTGCAG ATCAATCCTG ATTCTGCAGT TAATCCGGAA 
CATTTATCCT ATTTCCACTT TGTTGGACGA ATAATGGGAA TGGCTGTGTT TCATGGACAT 
TATATTGATG GTGGTTTCAC ATTGCCTTTT TATAAGCAAT TGCTTGGGAA GTCAATTACC 
TTGGATGACA TGGAGTTAGT AGATCCGGAT CTTCACAACA GTTTAGTGTG GATACTTGAG 
AATGATATTA CAGGTGTTTT GGACCATACC TTCTGTGTTG AACATAATGC ATATGGTGAA 
ATTATTCAGC ATGAACTTAA ACCAAATGGC AAAAGTATCC CTGTTAATGA AGAAAATAAA 
AAAGAATATG TCAGGCTCTA TGTGAACTGG AGATTTTTAC GAGGCATTGA GGCTCAATTC 
TTGGCTCTGC AGAAAGGATT TAATGAAGTA ATTCCACAAC ATCTGCTGAA GACATTTGAT 
GAGAAGGAGT TAGAGCTCAT TATTTGTGGA CTTGGAAAGA TAGATGTTAA TGACTGGAAG 
GTAAACACCC GGTTAAAACA CTGTACACCA GACAGCAACA TTGTCAAATG GTTCTGGAAA 
GCTGTGGAGT TTTTTGATGA AGAGCGACGA GCAAGATTGC TTCAGTTTGT GACAGGATCC 
TCTCGAGTGC CTCTGCAGGG CTTCAAAGCA TTGCAAGGTG CTGCAGGCCC GAGACTCTTT 
ACCATACACC AGATTGATGC CTGCACTAAC AACCTGCCGA AAGCCCACAC TTGCTTCAAT 
CGAATAGACA TTCCACCCTA TGAAAGCTAT GAAAAGCTAT ATGAAAAGCT GCTAACAGCC 
ATTGAAGAAA CATGTGGATT TGCTGTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523430.1
CDS	1..2253
Translation	MGLSSCRKTSSGLPLILHYVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

Target ORF information:

RefSeq Version	XM_011525128.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525128.1

ORF Insert Sequence:

ATGGGACTGT CTAGTTGCAG GAAAACAAGC TCAGGGCTCC CACTGATTCT ACATTATGTA 
CTCTGTGCAA AAAACCTGGT GAAAAAGGAT TTTTTCCGAC TTCCTGATCC ATTTGCTAAG 
GTGGTGGTTG ATGGATCTGG GCAATGCCAT TCTACAGATA CTGTGAAGAA TACGCTTGAT 
CCAAAGTGGA ATCAGCATTA TGACCTGTAT ATTGGAAAGT CTGATTCAGT TACGATCAGT 
GTATGGAATC ACAAGAAGAT CCATAAGAAA CAAGGTGCTG GATTTCTCGG TTGTGTTCGT 
CTTCTTTCCA ATGCCATCAA CCGCCTCAAA GACACTGGTT ATCAGAGGTT GGATTTATGC 
AAACTCGGGC CAAATGACAA TGATACAGTT AGAGGACAGA TAGTAGTAAG TCTTCAGTCC 
AGAGACCGAA TAGGCACAGG AGGACAAGTT GTGGACTGCA GTCGTTTATT TGATAACGAT 
TTACCAGACG GCTGGGAAGA AAGGAGAACC GCCTCTGGAA GAATCCAGTA TCTAAACCAT 
ATAACAAGAA CTACGCAATG GGAGCGCCCA ACACGACCGG CATCCGAATA TTCTAGCCCT 
GGCAGACCTC TTAGCTGCTT TGTTGATGAG AACACTCCAA TTAGTGGAAC AAATGGTGCA 
ACATGTGGAC AGTCTTCAGA TCCCAGGCTG GCAGAGAGGA GAGTCAGGTC ACAACGACAT 
AGAAATTACA TGAGCAGAAC ACATTTACAT ACTCCTCCAG ACCTACCAGA AGGCTATGAA 
CAGAGGACAA CGCAACAAGG CCAGGTGTAT TTCTTACATA CACAGACTGG TGTGAGCACA 
TGGCATGATC CAAGAGTGCC CAGGGATCTT AGCAACATCA ATTGTGAAGA GCTTGGTCCA 
TTGCCTCCTG GATGGGAGAT CCGTAATACG GCAACAGGCA GAGTTTATTT CGTTGACCAT 
AACAACAGAA CAACACAATT TACAGATCCT CGGCTGTCTG CTAACTTGCA TTTAGTTTTA 
AATCGGCAGA ACCAATTGAA AGACCAACAG CAACAGCAAG TGGTATCGTT ATGTCCTGAT 
GACACAGAAT GCCTGACAGT CCCAAGGTAC AAGCGAGACC TGGTTCAGAA ACTAAAAATT 
TTGCGGCAAG AACTTTCCCA ACAACAGCCT CAGGCAGGTC ATTGCCGCAT TGAGGTTTCC 
AGGGAAGAGA TTTTTGAGGA ATCATATCGA CAGGTCATGA AAATGAGACC AAAAGATCTC 
TGGAAGCGAT TAATGATAAA ATTTCGTGGA GAAGAAGGCC TTGACTATGG AGGCGTTGCC 
AGGGAATGGT TGTATCTCTT GTCACATGAA ATGTTGAATC CATACTATGG CCTCTTCCAG 
TATTCAAGAG ATGATATTTA TACATTGCAG ATCAATCCTG ATTCTGCAGT TAATCCGGAA 
CATTTATCCT ATTTCCACTT TGTTGGACGA ATAATGGGAA TGGCTGTGTT TCATGGACAT 
TATATTGATG GTGGTTTCAC ATTGCCTTTT TATAAGCAAT TGCTTGGGAA GTCAATTACC 
TTGGATGACA TGGAGTTAGT AGATCCGGAT CTTCACAACA GTTTAGTGTG GATACTTGAG 
AATGATATTA CAGGTGTTTT GGACCATACC TTCTGTGTTG AACATAATGC ATATGGTGAA 
ATTATTCAGC ATGAACTTAA ACCAAATGGC AAAAGTATCC CTGTTAATGA AGAAAATAAA 
AAAGAATATG TCAGGCTCTA TGTGAACTGG AGATTTTTAC GAGGCATTGA GGCTCAATTC 
TTGGCTCTGC AGAAAGGATT TAATGAAGTA ATTCCACAAC ATCTGCTGAA GACATTTGAT 
GAGAAGGAGT TAGAGCTCAT TATTTGTGGA CTTGGAAAGA TAGATGTTAA TGACTGGAAG 
GTAAACACCC GGTTAAAACA CTGTACACCA GACAGCAACA TTGTCAAATG GTTCTGGAAA 
GCTGTGGAGT TTTTTGATGA AGAGCGACGA GCAAGATTGC TTCAGTTTGT GACAGGATCC 
TCTCGAGTGC CTCTGCAGGG CTTCAAAGCA TTGCAAGGTG CTGCAGGCCC GAGACTCTTT 
ACCATACACC AGATTGATGC CTGCACTAAC AACCTGCCGA AAGCCCACAC TTGCTTCAAT 
CGAATAGACA TTCCACCCTA TGAAAGCTAT GAAAAGCTAT ATGAAAAGCT GCTAACAGCC 
ATTGAAGAAA CATGTGGATT TGCTGTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Smurf2, an E3 ubiquitin ligase, interacts with PDE4B and attenuates liver fibrosis through miR-132 mediated CTGF inhibition.
Biochimica et biophysica acta1865(2)297-308(2018 Feb)
Cai Y,Huang G,Ma L,Dong L,Chen S,Shen X,Zhang S,Xue R,Sun D,Zhang S

Smurf2-Mediated Stabilization of DNA Topoisomerase II? Controls Genomic Integrity.
Cancer research77(16)4217-4227(2017 Aug)
Emanuelli A,Borroni AP,Apel-Sarid L,Shah PA,Ayyathan DM,Koganti P,Levy-Cohen G,Blank M

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

The PIAS3-Smurf2 sumoylation pathway suppresses breast cancer organoid invasiveness.
Oncotarget8(13)21001-21014(2017 Mar)
Chandhoke AS,Chanda A,Karve K,Deng L,Bonni S

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SMURF2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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