NPFFR1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPFFR1
Entrez Gene ID	64106
Full Name	neuropeptide FF receptor 1
Synonyms	GPR147,NPFF1,NPFF1R1,OT7T022
General protein information	Preferred Names neuropeptide FF receptor 1 Names neuropeptide FF receptor 1 G-protein coupled receptor 147 RFamide-related peptide receptor OT7T022 neuropeptide FF 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q22.1
Disorder MIM:	607448

mRNA and Protein(s)

mRNA	Protein	Name
NM_022146.4	NP_071429.1	neuropeptide FF receptor 1
NM_022146.5	NP_071429.1	neuropeptide FF receptor 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NPFFR1	XP_002698951.1
Pan troglodytes (chimpanzee)	NPFFR1	XP_521501.3
	LOC716688	XP_001107552.2
Xenopus tropicalis (tropical clawed frog)	npffr1	XP_004915843.1
Canis lupus familiaris (dog)	NPFFR1	XP_851935.2
Homo sapiens (human)	NPFFR1	NP_071429.1
Mus musculus (house mouse)	Npffr1	NP_001170982.1
Rattus norvegicus (Norway rat)	Npffr1	NP_071627.2
Xenopus tropicalis (tropical clawed frog)	LOC100491949	XP_004920471.1
Gallus gallus (chicken)	NPFFR1	NP_989693.1
Danio rerio (zebrafish)	npffr1l3	NP_001082858.1
Danio rerio (zebrafish)	npffr1l2	NP_001165168.1

Gene Ontology
Bibliography

Related articles in PubMed

Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins.
Badgandi HB, Hwang SH, Shimada IS, Loriot E, Mukhopadhyay S
The Journal of cell biology216(3)743-760(2017 Mar)

Effects of systematic N-terminus deletions and benzoylations of endogenous RF-amide peptides on NPFF1R, NPFF2R, GPR10, GPR54 and GPR103.
Roum?as L, Humbert JP, Schneider S, Doebelin C, Bertin I, Schmitt M, Bourguignon JJ, Simonin F, Bihel F
Peptides71156-61(2015 Sep)

Mutational analysis of the genes encoding RFamide-related peptide-3, the human orthologue of gonadotrophin-inhibitory hormone, and its receptor (GPR147) in patients with gonadotrophin-releasing hormone-dependent pubertal disorders.
Lima CJ, Cardoso SC, Lemos EF, Zingler E, Capanema C, Menezes LD, Vogado G, Dos Santos BT, de Moraes OL, Duarte EF, de Brito VN, Latronico AC, Lofrano-Porto A
Journal of neuroendocrinology26(11)817-24(2014 Nov)

Genome-wide association study identifies germline polymorphisms associated with relapse of childhood acute lymphoblastic leukemia.
Yang JJ, Cheng C, Devidas M, Cao X, Campana D, Yang W, Fan Y, Neale G, Cox N, Scheet P, Borowitz MJ, Winick NJ, Martin PL, Bowman WP, Camitta B, Reaman GH, Carroll WL, Willman CL, Hunger SP, Evans WE, Pui CH, Loh M, Relling MV
Blood120(20)4197-204(2012 Nov)

The human gonadotropin-inhibitory hormone ortholog RFamide-related peptide-3 suppresses gonadotropin-induced progesterone production in human granulosa cells.
Oishi H, Klausen C, Bentley GE, Osugi T, Tsutsui K, Gilks CB, Yano T, Leung PC
Endocrinology153(7)3435-45(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Effects of systematic N-terminus deletions and benzoylations of endogenous RF-amide peptides on NPFF1R, NPFF2R, GPR10, GPR54 and GPR103.
Title: Effects of systematic N-terminus deletions and benzoylations of endogenous RF-amide peptides on NPFF1R, NPFF2R, GPR10, GPR54 and GPR103.

RFRP-3/GPR147 may play secondary, modulatory roles on the regulation of pubertal development; a restraining modulatory effect of the NPVF p.I71_K72 variant on the activation of the gonadotrophic axis cannot be ruled out
Title: Mutational analysis of the genes encoding RFamide-related peptide-3, the human orthologue of gonadotrophin-inhibitory hormone, and its receptor (GPR147) in patients with gonadotrophin-releasing hormone-dependent pubertal disorders.

ovarian RFRP-3/GPR147 signaling could contribute to normal ovarian function
Title: The human gonadotropin-inhibitory hormone ortholog RFamide-related peptide-3 suppresses gonadotropin-induced progesterone production in human granulosa cells.

In male rats, mRNA expressions of both RFRP and GPR147 increases from postnatal days 12 and 16; mRNA expression of GPR147 in female rats increases from postnatal day 16, peaking at postnatal day 28 and decreasing from postnatal day 35.
Title: Developmental changes in the mammalian gonadotropin-inhibitory hormone (GnIH) ortholog RFamide-related peptide (RFRP) and its cognate receptor GPR147 in the rat hypothalamus.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following NPFFR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPFFR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01562	NM_022146.5 Latest version!	Homo sapiens neuropeptide FF receptor 1 (NPFFR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$179.50-$251.30 $359.00
View Old Accession Versions >>
OHu01562	NM_022146.4	Homo sapiens neuropeptide FF receptor 1 (NPFFR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$179.50-$251.30 $359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPFFR1 ( NM_022146.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_071429.1 Homo sapiens neuropeptide FF receptor 1 (NPFFR1), mRNA.

Price & Availability↑

CloneID	OHu01562
Clone ID Related Accession (Same CDS sequence)	NM_022146.4 , NM_022146.5
Accession Version	NM_022146.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	neuropeptide FF receptor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355138.19 and EU432128.1. On Jul 17, 2010 this sequence version replaced NM_022146.3. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.262003.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGGGG AGCCCTCCCA GCCTCCCAAC AGCAGTTGGC CCCTAAGTCA GAATGGGACT 
AACACTGAGG CCACCCCGGC TACAAACCTC ACCTTCTCCT CCTACTATCA GCACACCTCC 
CCTGTGGCGG CCATGTTCAT TGTGGCCTAT GCGCTCATCT TCCTGCTCTG CATGGTGGGC 
AACACCCTGG TCTGTTTCAT CGTGCTCAAG AACCGGCACA TGCATACTGT CACCAACATG 
TTCATCCTCA ACCTGGCTGT CAGTGACCTG CTGGTGGGCA TCTTCTGCAT GCCCACCACC 
CTTGTGGACA ACCTCATCAC TGGGTGGCCC TTCGACAATG CCACATGCAA GATGAGCGGC 
TTGGTGCAGG GCATGTCTGT GTCGGCTTCC GTTTTCACAC TGGTGGCCAT TGCTGTGGAA 
AGGTTCCGCT GCATCGTGCA CCCTTTCCGC GAGAAGCTGA CCCTGCGGAA GGCGCTCGTC 
ACCATCGCCG TCATCTGGGC CCTGGCGCTG CTCATCATGT GTCCCTCGGC CGTCACGCTG 
ACCGTCACCC GTGAGGAGCA CCACTTCATG GTGGACGCCC GCAACCGCTC CTACCCGCTC 
TACTCCTGCT GGGAGGCCTG GCCCGAGAAG GGCATGCGCA GGGTCTACAC CACTGTGCTC 
TTCTCGCACA TCTACCTGGC GCCGCTGGCG CTCATCGTGG TCATGTACGC CCGCATCGCG 
CGCAAGCTCT GCCAGGCCCC GGGCCCGGCC CCCGGGGGCG AGGAGGCTGC GGACCCGCGA 
GCATCGCGGC GCAGAGCGCG CGTGGTGCAC ATGCTGGTCA TGGTGGCGCT GTTCTTCACG 
CTGTCCTGGC TGCCGCTCTG GGCGCTGCTG CTGCTCATCG ACTACGGGCA GCTCAGCGCG 
CCGCAGCTGC ACCTGGTCAC CGTCTACGCC TTCCCCTTCG CGCACTGGCT GGCCTTCTTC 
AACAGCAGCG CCAACCCCAT CATCTACGGC TACTTCAACG AGAACTTCCG CCGCGGCTTC 
CAGGCCGCCT TCCGCGCCCG CCTCTGCCCG CGCCCGTCGG GGAGCCACAA GGAGGCCTAC 
TCCGAGCGGC CCGGCGGGCT TCTGCACAGG CGGGTCTTCG TGGTGGTGCG GCCCAGCGAC 
TCCGGGCTGC CCTCTGAGTC GGGCCCTAGC AGTGGGGCCC CCAGGCCCGG CCGCCTCCCG 
CTGCGGAATG GGCGGGTGGC TCACCACGGC TTGCCCAGGG AAGGGCCTGG CTGCTCCCAC 
CTGCCCCTCA CCATTCCAGC CTGGGATATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071429.1
CDS	19..1311
Translation	MEGEPSQPPNSSWPLSQNGTNTEATPATNLTFSSYYQHTSPVAAMFIVAYALIFLLCMVGNTLVCFIVLKNRHMHTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITGWPFDNATCKMSGLVQGMSVSASVFTLVAIAVERFRCIVHPFREKLTLRKALVTIAVIWALALLIMCPSAVTLTVTREEHHFMVDARNRSYPLYSCWEAWPEKGMRRVYTTVLFSHIYLAPLALIVVMYARIARKLCQAPGPAPGGEEAADPRASRRRARVVHMLVMVALFFTLSWLPLWALLLLIDYGQLSAPQLHLVTVYAFPFAHWLAFFNSSANPIIYGYFNENFRRGFQAAFRARLCPRPSGSHKEAYSERPGGLLHRRVFVVVRPSDSGLPSESGPSSGAPRPGRLPLRNGRVAHHGLPREGPGCSHLPLTIPAWDI

Target ORF information:

RefSeq Version	NM_022146.4
Organism	Homo sapiens(human)
Definition	Homo sapiens neuropeptide FF receptor 1 (NPFFR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022146.4

ORF Insert Sequence:

ATGGAGGGGG AGCCCTCCCA GCCTCCCAAC AGCAGTTGGC CCCTAAGTCA GAATGGGACT 
AACACTGAGG CCACCCCGGC TACAAACCTC ACCTTCTCCT CCTACTATCA GCACACCTCC 
CCTGTGGCGG CCATGTTCAT TGTGGCCTAT GCGCTCATCT TCCTGCTCTG CATGGTGGGC 
AACACCCTGG TCTGTTTCAT CGTGCTCAAG AACCGGCACA TGCATACTGT CACCAACATG 
TTCATCCTCA ACCTGGCTGT CAGTGACCTG CTGGTGGGCA TCTTCTGCAT GCCCACCACC 
CTTGTGGACA ACCTCATCAC TGGGTGGCCC TTCGACAATG CCACATGCAA GATGAGCGGC 
TTGGTGCAGG GCATGTCTGT GTCGGCTTCC GTTTTCACAC TGGTGGCCAT TGCTGTGGAA 
AGGTTCCGCT GCATCGTGCA CCCTTTCCGC GAGAAGCTGA CCCTGCGGAA GGCGCTCGTC 
ACCATCGCCG TCATCTGGGC CCTGGCGCTG CTCATCATGT GTCCCTCGGC CGTCACGCTG 
ACCGTCACCC GTGAGGAGCA CCACTTCATG GTGGACGCCC GCAACCGCTC CTACCCGCTC 
TACTCCTGCT GGGAGGCCTG GCCCGAGAAG GGCATGCGCA GGGTCTACAC CACTGTGCTC 
TTCTCGCACA TCTACCTGGC GCCGCTGGCG CTCATCGTGG TCATGTACGC CCGCATCGCG 
CGCAAGCTCT GCCAGGCCCC GGGCCCGGCC CCCGGGGGCG AGGAGGCTGC GGACCCGCGA 
GCATCGCGGC GCAGAGCGCG CGTGGTGCAC ATGCTGGTCA TGGTGGCGCT GTTCTTCACG 
CTGTCCTGGC TGCCGCTCTG GGCGCTGCTG CTGCTCATCG ACTACGGGCA GCTCAGCGCG 
CCGCAGCTGC ACCTGGTCAC CGTCTACGCC TTCCCCTTCG CGCACTGGCT GGCCTTCTTC 
AACAGCAGCG CCAACCCCAT CATCTACGGC TACTTCAACG AGAACTTCCG CCGCGGCTTC 
CAGGCCGCCT TCCGCGCCCG CCTCTGCCCG CGCCCGTCGG GGAGCCACAA GGAGGCCTAC 
TCCGAGCGGC CCGGCGGGCT TCTGCACAGG CGGGTCTTCG TGGTGGTGCG GCCCAGCGAC 
TCCGGGCTGC CCTCTGAGTC GGGCCCTAGC AGTGGGGCCC CCAGGCCCGG CCGCCTCCCG 
CTGCGGAATG GGCGGGTGGC TCACCACGGC TTGCCCAGGG AAGGGCCTGG CTGCTCCCAC 
CTGCCCCTCA CCATTCCAGC CTGGGATATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPFFR1 ( NM_022146.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_071429.1 Homo sapiens neuropeptide FF receptor 1 (NPFFR1), mRNA.

Price & Availability↑

CloneID	OHu01562
Clone ID Related Accession (Same CDS sequence)	NM_022146.4 , NM_022146.5
Accession Version	NM_022146.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	neuropeptide FF receptor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355138.19 and EU432128.1. On May 17, 2019 this sequence version replaced NM_022146.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.262003.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000277942.7/ ENSP00000277942.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGAGGGGG AGCCCTCCCA GCCTCCCAAC AGCAGTTGGC CCCTAAGTCA GAATGGGACT 
AACACTGAGG CCACCCCGGC TACAAACCTC ACCTTCTCCT CCTACTATCA GCACACCTCC 
CCTGTGGCGG CCATGTTCAT TGTGGCCTAT GCGCTCATCT TCCTGCTCTG CATGGTGGGC 
AACACCCTGG TCTGTTTCAT CGTGCTCAAG AACCGGCACA TGCATACTGT CACCAACATG 
TTCATCCTCA ACCTGGCTGT CAGTGACCTG CTGGTGGGCA TCTTCTGCAT GCCCACCACC 
CTTGTGGACA ACCTCATCAC TGGGTGGCCC TTCGACAATG CCACATGCAA GATGAGCGGC 
TTGGTGCAGG GCATGTCTGT GTCGGCTTCC GTTTTCACAC TGGTGGCCAT TGCTGTGGAA 
AGGTTCCGCT GCATCGTGCA CCCTTTCCGC GAGAAGCTGA CCCTGCGGAA GGCGCTCGTC 
ACCATCGCCG TCATCTGGGC CCTGGCGCTG CTCATCATGT GTCCCTCGGC CGTCACGCTG 
ACCGTCACCC GTGAGGAGCA CCACTTCATG GTGGACGCCC GCAACCGCTC CTACCCGCTC 
TACTCCTGCT GGGAGGCCTG GCCCGAGAAG GGCATGCGCA GGGTCTACAC CACTGTGCTC 
TTCTCGCACA TCTACCTGGC GCCGCTGGCG CTCATCGTGG TCATGTACGC CCGCATCGCG 
CGCAAGCTCT GCCAGGCCCC GGGCCCGGCC CCCGGGGGCG AGGAGGCTGC GGACCCGCGA 
GCATCGCGGC GCAGAGCGCG CGTGGTGCAC ATGCTGGTCA TGGTGGCGCT GTTCTTCACG 
CTGTCCTGGC TGCCGCTCTG GGCGCTGCTG CTGCTCATCG ACTACGGGCA GCTCAGCGCG 
CCGCAGCTGC ACCTGGTCAC CGTCTACGCC TTCCCCTTCG CGCACTGGCT GGCCTTCTTC 
AACAGCAGCG CCAACCCCAT CATCTACGGC TACTTCAACG AGAACTTCCG CCGCGGCTTC 
CAGGCCGCCT TCCGCGCCCG CCTCTGCCCG CGCCCGTCGG GGAGCCACAA GGAGGCCTAC 
TCCGAGCGGC CCGGCGGGCT TCTGCACAGG CGGGTCTTCG TGGTGGTGCG GCCCAGCGAC 
TCCGGGCTGC CCTCTGAGTC GGGCCCTAGC AGTGGGGCCC CCAGGCCCGG CCGCCTCCCG 
CTGCGGAATG GGCGGGTGGC TCACCACGGC TTGCCCAGGG AAGGGCCTGG CTGCTCCCAC 
CTGCCCCTCA CCATTCCAGC CTGGGATATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071429.1
CDS	329..1621
Translation	MEGEPSQPPNSSWPLSQNGTNTEATPATNLTFSSYYQHTSPVAAMFIVAYALIFLLCMVGNTLVCFIVLKNRHMHTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITGWPFDNATCKMSGLVQGMSVSASVFTLVAIAVERFRCIVHPFREKLTLRKALVTIAVIWALALLIMCPSAVTLTVTREEHHFMVDARNRSYPLYSCWEAWPEKGMRRVYTTVLFSHIYLAPLALIVVMYARIARKLCQAPGPAPGGEEAADPRASRRRARVVHMLVMVALFFTLSWLPLWALLLLIDYGQLSAPQLHLVTVYAFPFAHWLAFFNSSANPIIYGYFNENFRRGFQAAFRARLCPRPSGSHKEAYSERPGGLLHRRVFVVVRPSDSGLPSESGPSSGAPRPGRLPLRNGRVAHHGLPREGPGCSHLPLTIPAWDI

Target ORF information:

RefSeq Version	NM_022146.5
Organism	Homo sapiens(human)
Definition	Homo sapiens neuropeptide FF receptor 1 (NPFFR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022146.5

ORF Insert Sequence:

ATGGAGGGGG AGCCCTCCCA GCCTCCCAAC AGCAGTTGGC CCCTAAGTCA GAATGGGACT 
AACACTGAGG CCACCCCGGC TACAAACCTC ACCTTCTCCT CCTACTATCA GCACACCTCC 
CCTGTGGCGG CCATGTTCAT TGTGGCCTAT GCGCTCATCT TCCTGCTCTG CATGGTGGGC 
AACACCCTGG TCTGTTTCAT CGTGCTCAAG AACCGGCACA TGCATACTGT CACCAACATG 
TTCATCCTCA ACCTGGCTGT CAGTGACCTG CTGGTGGGCA TCTTCTGCAT GCCCACCACC 
CTTGTGGACA ACCTCATCAC TGGGTGGCCC TTCGACAATG CCACATGCAA GATGAGCGGC 
TTGGTGCAGG GCATGTCTGT GTCGGCTTCC GTTTTCACAC TGGTGGCCAT TGCTGTGGAA 
AGGTTCCGCT GCATCGTGCA CCCTTTCCGC GAGAAGCTGA CCCTGCGGAA GGCGCTCGTC 
ACCATCGCCG TCATCTGGGC CCTGGCGCTG CTCATCATGT GTCCCTCGGC CGTCACGCTG 
ACCGTCACCC GTGAGGAGCA CCACTTCATG GTGGACGCCC GCAACCGCTC CTACCCGCTC 
TACTCCTGCT GGGAGGCCTG GCCCGAGAAG GGCATGCGCA GGGTCTACAC CACTGTGCTC 
TTCTCGCACA TCTACCTGGC GCCGCTGGCG CTCATCGTGG TCATGTACGC CCGCATCGCG 
CGCAAGCTCT GCCAGGCCCC GGGCCCGGCC CCCGGGGGCG AGGAGGCTGC GGACCCGCGA 
GCATCGCGGC GCAGAGCGCG CGTGGTGCAC ATGCTGGTCA TGGTGGCGCT GTTCTTCACG 
CTGTCCTGGC TGCCGCTCTG GGCGCTGCTG CTGCTCATCG ACTACGGGCA GCTCAGCGCG 
CCGCAGCTGC ACCTGGTCAC CGTCTACGCC TTCCCCTTCG CGCACTGGCT GGCCTTCTTC 
AACAGCAGCG CCAACCCCAT CATCTACGGC TACTTCAACG AGAACTTCCG CCGCGGCTTC 
CAGGCCGCCT TCCGCGCCCG CCTCTGCCCG CGCCCGTCGG GGAGCCACAA GGAGGCCTAC 
TCCGAGCGGC CCGGCGGGCT TCTGCACAGG CGGGTCTTCG TGGTGGTGCG GCCCAGCGAC 
TCCGGGCTGC CCTCTGAGTC GGGCCCTAGC AGTGGGGCCC CCAGGCCCGG CCGCCTCCCG 
CTGCGGAATG GGCGGGTGGC TCACCACGGC TTGCCCAGGG AAGGGCCTGG CTGCTCCCAC 
CTGCCCCTCA CCATTCCAGC CTGGGATATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins.
The Journal of cell biology216(3)743-760(2017 Mar)
Badgandi HB,Hwang SH,Shimada IS,Loriot E,Mukhopadhyay S

Effects of systematic N-terminus deletions and benzoylations of endogenous RF-amide peptides on NPFF1R, NPFF2R, GPR10, GPR54 and GPR103.
Peptides71156-61(2015 Sep)
Roum?as L,Humbert JP,Schneider S,Doebelin C,Bertin I,Schmitt M,Bourguignon JJ,Simonin F,Bihel F

Mutational analysis of the genes encoding RFamide-related peptide-3, the human orthologue of gonadotrophin-inhibitory hormone, and its receptor (GPR147) in patients with gonadotrophin-releasing hormone-dependent pubertal disorders.
Journal of neuroendocrinology26(11)817-24(2014 Nov)
Lima CJ,Cardoso SC,Lemos EF,Zingler E,Capanema C,Menezes LD,Vogado G,Dos Santos BT,de Moraes OL,Duarte EF,de Brito VN,Latronico AC,Lofrano-Porto A

Genome-wide association study identifies germline polymorphisms associated with relapse of childhood acute lymphoblastic leukemia.
Blood120(20)4197-204(2012 Nov)
Yang JJ,Cheng C,Devidas M,Cao X,Campana D,Yang W,Fan Y,Neale G,Cox N,Scheet P,Borowitz MJ,Winick NJ,Martin PL,Bowman WP,Camitta B,Reaman GH,Carroll WL,Willman CL,Hunger SP,Evans WE,Pui CH,Loh M,Relling MV

The human gonadotropin-inhibitory hormone ortholog RFamide-related peptide-3 suppresses gonadotropin-induced progesterone production in human granulosa cells.
Endocrinology153(7)3435-45(2012 Jul)
Oishi H,Klausen C,Bentley GE,Osugi T,Tsutsui K,Gilks CB,Yano T,Leung PC