ACTB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACTB
Entrez Gene ID	60
Full Name	actin beta
Synonyms	BRWS1,PS1TP5BP1
General protein information	Preferred Names actin beta Names actin, cytoplasmic 1 PS1TP5-binding protein 1 beta cytoskeletal actin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p22.1
Summary	This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017].
Disorder MIM:	102630
Disorder Html:	?Dystonia, juvenile-onset, 607371 (3); Baraitser-Winter syndrome 1, 243310 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001101.4	NP_001092.1	actin, cytoplasmic 1
NM_001101.3	NP_001092.1	actin, cytoplasmic 1
NM_001101.5	NP_001092.1	actin, cytoplasmic 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACTB	NP_001092.1
Gallus gallus (chicken)	ACTB	NP_990849.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011516	XP_315269.4
Pan troglodytes (chimpanzee)	ACTB	NP_001009945.1
	LOC100428865	XP_002802770.1
Rattus norvegicus (Norway rat)	Actb	NP_112406.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005095	XP_313971.4
Oryza sativa (rice)	Os05g0438800	NP_001055661.1
Canis lupus familiaris (dog)	ACTB	NP_001182774.1
Drosophila melanogaster (fruit fly)	Act57B	NP_523800.1
Xenopus tropicalis (tropical clawed frog)	actb	NP_998884.1
	ACTB	NP_001028256.1
Mus musculus (house mouse)	Actb	NP_031419.1
Bos taurus (cattle)	ACTB	NP_776404.2
Drosophila melanogaster (fruit fly)	Act87E	NP_731812.1

Gene Ontology
Bibliography

Related articles in PubMed

A Distinct Malignant Epithelioid Neoplasm With GLI1 Gene Rearrangements, Frequent S100 Protein Expression, and Metastatic Potential: Expanding the Spectrum of Pathologic Entities With ACTB/MALAT1/PTCH1-GLI1 Fusions.
Antonescu CR, Agaram NP, Sung YS, Zhang L, Swanson D, Dickson BC
The American journal of surgical pathology(2018 Jan)

ROS and glutathionylation balance cytoskeletal dynamics in neutrophil extracellular trap formation.
Stojkov D, Amini P, Oberson K, Sokollik C, Duppenthaler A, Simon HU, Yousefi S
The Journal of cell biology216(12)4073-4090(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors describe heterozygous ACTB deletions and nonsense and frameshift mutations in 33 individuals with developmental delay, apparent intellectual disability, increased frequency of internal organ malformations (including those of the heart and the renal tract), growth retardation, and a recognizable facial gestalt.
Title: ACTB Loss-of-Function Mutations Result in a Pleiotropic Developmental Disorder.

Data indicate AIM1 (absent in melanoma 1) as an actin binding protein and show that it regulates cytoskeletal remodeling and cell migration in prostate epithelial cells.
Title: AIM1 is an actin-binding protein that suppresses cell migration and micrometastatic dissemination.

Data suggest that, in T-lymphocytes, nitric oxide generated by eNOS S-nitrosylates Cys374 on ACTB and thus regulates activation/recruitment of PRKCQ at immune synapse; S-nitrosylation of beta-actin impairs actin binding to PFN1 and regulates protein transport in lamellipodia. (eNOS = nitric oxide synthase 3; ACTB = beta-actin; PRKCQ = protein kinase C-theta; PFN1 = profilin-1)
Title: eNOS S-nitrosylates ?-actin on Cys374 and regulates PKC-? at the immune synapse by impairing actin binding to profilin-1.

Based on present and published dup7p22.1 patients we suggest that renal abnormalities might be an additional feature of the 7p22.1 microduplication syndrome.We also pinpoint the ACTB gene as the key gene affecting the 7p22.1 duplication syndrome phenotype.
Title: 7p22.1 microduplication syndrome: Refinement of the critical region.

Data indicate that the IQGAP1 N-terminal fragment spanning residues 1-191 (CHDF) binds to both F-actin and Ca(2+)/calmodulin.
Title: The IQGAP1 N-Terminus Forms Dimers, and the Dimer Interface Is Required for Binding F-Actin and Calcium-Bound Calmodulin.

The following ACTB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19489	NM_001101.5 Latest version!	Homo sapiens actin beta (ACTB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu19489	NM_001101.3	Homo sapiens actin beta (ACTB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu19489	NM_001101.4	Homo sapiens actin beta (ACTB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACTB ( NM_001101.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092.1 Homo sapiens actin beta (ACTB), mRNA.

Price & Availability↑

CloneID	OHu19489
Clone ID Related Accession (Same CDS sequence)	NM_001101.4 , NM_001101.3 , NM_001101.5
Accession Version	NM_001101.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	actin, cytoplasmic 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC327656.1, AK130157.1, BC009636.1 and BU736098.1. On Sep 13, 2017 this sequence version replaced NM_001101.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.119918.1, AK025375.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGATG ATATCGCCGC GCTCGTCGTC GACAACGGCT CCGGCATGTG CAAGGCCGGC 
TTCGCGGGCG ACGATGCCCC CCGGGCCGTC TTCCCCTCCA TCGTGGGGCG CCCCAGGCAC 
CAGGGCGTGA TGGTGGGCAT GGGTCAGAAG GATTCCTATG TGGGCGACGA GGCCCAGAGC 
AAGAGAGGCA TCCTCACCCT GAAGTACCCC ATCGAGCACG GCATCGTCAC CAACTGGGAC 
GACATGGAGA AAATCTGGCA CCACACCTTC TACAATGAGC TGCGTGTGGC TCCCGAGGAG 
CACCCCGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACCGCGA GAAGATGACC 
CAGATCATGT TTGAGACCTT CAACACCCCA GCCATGTACG TTGCTATCCA GGCTGTGCTA 
TCCCTGTACG CCTCTGGCCG TACCACTGGC ATCGTGATGG ACTCCGGTGA CGGGGTCACC 
CACACTGTGC CCATCTACGA GGGGTATGCC CTCCCCCATG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACTGA CTACCTCATG AAGATCCTCA CCGAGCGCGG CTACAGCTTC 
ACCACCACGG CCGAGCGGGA AATCGTGCGT GACATTAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAAGAGAT GGCCACGGCT GCTTCCAGCT CCTCCCTGGA GAAGAGCTAC 
GAGCTGCCTG ACGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGCTG CCCTGAGGCA 
CTCTTCCAGC CTTCCTTCCT GGGCATGGAG TCCTGTGGCA TCCACGAAAC TACCTTCAAC 
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC AGTGCTGTCT 
GGCGGCACCA CCATGTACCC TGGCATTGCC GACAGGATGC AGAAGGAGAT CACTGCCCTG 
GCACCCAGCA CAATGAAGAT CAAGATCATT GCTCCTCCTG AGCGCAAGTA CTCCGTGTGG 
ATCGGCGGCT CCATCCTGGC CTCGCTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTATGACG AGTCCGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092.1
CDS	193..1320
Translation	MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001101.4
Organism	Homo sapiens(human)
Definition	Homo sapiens actin beta (ACTB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101.4

ORF Insert Sequence:

ATGGATGATG ATATCGCCGC GCTCGTCGTC GACAACGGCT CCGGCATGTG CAAGGCCGGC 
TTCGCGGGCG ACGATGCCCC CCGGGCCGTC TTCCCCTCCA TCGTGGGGCG CCCCAGGCAC 
CAGGGCGTGA TGGTGGGCAT GGGTCAGAAG GATTCCTATG TGGGCGACGA GGCCCAGAGC 
AAGAGAGGCA TCCTCACCCT GAAGTACCCC ATCGAGCACG GCATCGTCAC CAACTGGGAC 
GACATGGAGA AAATCTGGCA CCACACCTTC TACAATGAGC TGCGTGTGGC TCCCGAGGAG 
CACCCCGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACCGCGA GAAGATGACC 
CAGATCATGT TTGAGACCTT CAACACCCCA GCCATGTACG TTGCTATCCA GGCTGTGCTA 
TCCCTGTACG CCTCTGGCCG TACCACTGGC ATCGTGATGG ACTCCGGTGA CGGGGTCACC 
CACACTGTGC CCATCTACGA GGGGTATGCC CTCCCCCATG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACTGA CTACCTCATG AAGATCCTCA CCGAGCGCGG CTACAGCTTC 
ACCACCACGG CCGAGCGGGA AATCGTGCGT GACATTAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAAGAGAT GGCCACGGCT GCTTCCAGCT CCTCCCTGGA GAAGAGCTAC 
GAGCTGCCTG ACGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGCTG CCCTGAGGCA 
CTCTTCCAGC CTTCCTTCCT GGGCATGGAG TCCTGTGGCA TCCACGAAAC TACCTTCAAC 
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC AGTGCTGTCT 
GGCGGCACCA CCATGTACCC TGGCATTGCC GACAGGATGC AGAAGGAGAT CACTGCCCTG 
GCACCCAGCA CAATGAAGAT CAAGATCATT GCTCCTCCTG AGCGCAAGTA CTCCGTGTGG 
ATCGGCGGCT CCATCCTGGC CTCGCTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTATGACG AGTCCGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTB ( NM_001101.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092.1 Homo sapiens actin beta (ACTB), mRNA.

Price & Availability↑

CloneID	OHu19489
Clone ID Related Accession (Same CDS sequence)	NM_001101.4 , NM_001101.3 , NM_001101.5
Accession Version	NM_001101.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-05
Organism	Homo sapiens(Human)
Product	actin, cytoplasmic 1
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK130157.1 and BC009636.1. This sequence is a reference standard in the RefSeqGene project. On Feb 22, 2008 this sequence version replaced NM_001101.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222925.1, BC014861.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGATG ATATCGCCGC GCTCGTCGTC GACAACGGCT CCGGCATGTG CAAGGCCGGC 
TTCGCGGGCG ACGATGCCCC CCGGGCCGTC TTCCCCTCCA TCGTGGGGCG CCCCAGGCAC 
CAGGGCGTGA TGGTGGGCAT GGGTCAGAAG GATTCCTATG TGGGCGACGA GGCCCAGAGC 
AAGAGAGGCA TCCTCACCCT GAAGTACCCC ATCGAGCACG GCATCGTCAC CAACTGGGAC 
GACATGGAGA AAATCTGGCA CCACACCTTC TACAATGAGC TGCGTGTGGC TCCCGAGGAG 
CACCCCGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACCGCGA GAAGATGACC 
CAGATCATGT TTGAGACCTT CAACACCCCA GCCATGTACG TTGCTATCCA GGCTGTGCTA 
TCCCTGTACG CCTCTGGCCG TACCACTGGC ATCGTGATGG ACTCCGGTGA CGGGGTCACC 
CACACTGTGC CCATCTACGA GGGGTATGCC CTCCCCCATG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACTGA CTACCTCATG AAGATCCTCA CCGAGCGCGG CTACAGCTTC 
ACCACCACGG CCGAGCGGGA AATCGTGCGT GACATTAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAAGAGAT GGCCACGGCT GCTTCCAGCT CCTCCCTGGA GAAGAGCTAC 
GAGCTGCCTG ACGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGCTG CCCTGAGGCA 
CTCTTCCAGC CTTCCTTCCT GGGCATGGAG TCCTGTGGCA TCCACGAAAC TACCTTCAAC 
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC AGTGCTGTCT 
GGCGGCACCA CCATGTACCC TGGCATTGCC GACAGGATGC AGAAGGAGAT CACTGCCCTG 
GCACCCAGCA CAATGAAGAT CAAGATCATT GCTCCTCCTG AGCGCAAGTA CTCCGTGTGG 
ATCGGCGGCT CCATCCTGGC CTCGCTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTATGACG AGTCCGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092.1
CDS	85..1212
Translation	MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001101.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens actin beta (ACTB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101.3

ORF Insert Sequence:

ATGGATGATG ATATCGCCGC GCTCGTCGTC GACAACGGCT CCGGCATGTG CAAGGCCGGC 
TTCGCGGGCG ACGATGCCCC CCGGGCCGTC TTCCCCTCCA TCGTGGGGCG CCCCAGGCAC 
CAGGGCGTGA TGGTGGGCAT GGGTCAGAAG GATTCCTATG TGGGCGACGA GGCCCAGAGC 
AAGAGAGGCA TCCTCACCCT GAAGTACCCC ATCGAGCACG GCATCGTCAC CAACTGGGAC 
GACATGGAGA AAATCTGGCA CCACACCTTC TACAATGAGC TGCGTGTGGC TCCCGAGGAG 
CACCCCGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACCGCGA GAAGATGACC 
CAGATCATGT TTGAGACCTT CAACACCCCA GCCATGTACG TTGCTATCCA GGCTGTGCTA 
TCCCTGTACG CCTCTGGCCG TACCACTGGC ATCGTGATGG ACTCCGGTGA CGGGGTCACC 
CACACTGTGC CCATCTACGA GGGGTATGCC CTCCCCCATG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACTGA CTACCTCATG AAGATCCTCA CCGAGCGCGG CTACAGCTTC 
ACCACCACGG CCGAGCGGGA AATCGTGCGT GACATTAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAAGAGAT GGCCACGGCT GCTTCCAGCT CCTCCCTGGA GAAGAGCTAC 
GAGCTGCCTG ACGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGCTG CCCTGAGGCA 
CTCTTCCAGC CTTCCTTCCT GGGCATGGAG TCCTGTGGCA TCCACGAAAC TACCTTCAAC 
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC AGTGCTGTCT 
GGCGGCACCA CCATGTACCC TGGCATTGCC GACAGGATGC AGAAGGAGAT CACTGCCCTG 
GCACCCAGCA CAATGAAGAT CAAGATCATT GCTCCTCCTG AGCGCAAGTA CTCCGTGTGG 
ATCGGCGGCT CCATCCTGGC CTCGCTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTATGACG AGTCCGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTB ( NM_001101.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092.1 Homo sapiens actin beta (ACTB), mRNA.

Price & Availability↑

CloneID	OHu19489
Clone ID Related Accession (Same CDS sequence)	NM_001101.4 , NM_001101.3 , NM_001101.5
Accession Version	NM_001101.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	actin, cytoplasmic 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK130157.1 and BC009636.1. On Nov 23, 2018 this sequence version replaced NM_001101.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222925.1, SRR3476690.1161360.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000646664.1/ ENSP00000494750.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATGATG ATATCGCCGC GCTCGTCGTC GACAACGGCT CCGGCATGTG CAAGGCCGGC 
TTCGCGGGCG ACGATGCCCC CCGGGCCGTC TTCCCCTCCA TCGTGGGGCG CCCCAGGCAC 
CAGGGCGTGA TGGTGGGCAT GGGTCAGAAG GATTCCTATG TGGGCGACGA GGCCCAGAGC 
AAGAGAGGCA TCCTCACCCT GAAGTACCCC ATCGAGCACG GCATCGTCAC CAACTGGGAC 
GACATGGAGA AAATCTGGCA CCACACCTTC TACAATGAGC TGCGTGTGGC TCCCGAGGAG 
CACCCCGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACCGCGA GAAGATGACC 
CAGATCATGT TTGAGACCTT CAACACCCCA GCCATGTACG TTGCTATCCA GGCTGTGCTA 
TCCCTGTACG CCTCTGGCCG TACCACTGGC ATCGTGATGG ACTCCGGTGA CGGGGTCACC 
CACACTGTGC CCATCTACGA GGGGTATGCC CTCCCCCATG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACTGA CTACCTCATG AAGATCCTCA CCGAGCGCGG CTACAGCTTC 
ACCACCACGG CCGAGCGGGA AATCGTGCGT GACATTAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAAGAGAT GGCCACGGCT GCTTCCAGCT CCTCCCTGGA GAAGAGCTAC 
GAGCTGCCTG ACGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGCTG CCCTGAGGCA 
CTCTTCCAGC CTTCCTTCCT GGGCATGGAG TCCTGTGGCA TCCACGAAAC TACCTTCAAC 
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC AGTGCTGTCT 
GGCGGCACCA CCATGTACCC TGGCATTGCC GACAGGATGC AGAAGGAGAT CACTGCCCTG 
GCACCCAGCA CAATGAAGAT CAAGATCATT GCTCCTCCTG AGCGCAAGTA CTCCGTGTGG 
ATCGGCGGCT CCATCCTGGC CTCGCTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTATGACG AGTCCGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092.1
CDS	85..1212
Translation	MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001101.5
Organism	Homo sapiens(human)
Definition	Homo sapiens actin beta (ACTB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101.5

ORF Insert Sequence:

ATGGATGATG ATATCGCCGC GCTCGTCGTC GACAACGGCT CCGGCATGTG CAAGGCCGGC 
TTCGCGGGCG ACGATGCCCC CCGGGCCGTC TTCCCCTCCA TCGTGGGGCG CCCCAGGCAC 
CAGGGCGTGA TGGTGGGCAT GGGTCAGAAG GATTCCTATG TGGGCGACGA GGCCCAGAGC 
AAGAGAGGCA TCCTCACCCT GAAGTACCCC ATCGAGCACG GCATCGTCAC CAACTGGGAC 
GACATGGAGA AAATCTGGCA CCACACCTTC TACAATGAGC TGCGTGTGGC TCCCGAGGAG 
CACCCCGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACCGCGA GAAGATGACC 
CAGATCATGT TTGAGACCTT CAACACCCCA GCCATGTACG TTGCTATCCA GGCTGTGCTA 
TCCCTGTACG CCTCTGGCCG TACCACTGGC ATCGTGATGG ACTCCGGTGA CGGGGTCACC 
CACACTGTGC CCATCTACGA GGGGTATGCC CTCCCCCATG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACTGA CTACCTCATG AAGATCCTCA CCGAGCGCGG CTACAGCTTC 
ACCACCACGG CCGAGCGGGA AATCGTGCGT GACATTAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAAGAGAT GGCCACGGCT GCTTCCAGCT CCTCCCTGGA GAAGAGCTAC 
GAGCTGCCTG ACGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGCTG CCCTGAGGCA 
CTCTTCCAGC CTTCCTTCCT GGGCATGGAG TCCTGTGGCA TCCACGAAAC TACCTTCAAC 
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC AGTGCTGTCT 
GGCGGCACCA CCATGTACCC TGGCATTGCC GACAGGATGC AGAAGGAGAT CACTGCCCTG 
GCACCCAGCA CAATGAAGAT CAAGATCATT GCTCCTCCTG AGCGCAAGTA CTCCGTGTGG 
ATCGGCGGCT CCATCCTGGC CTCGCTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTATGACG AGTCCGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Distinct Malignant Epithelioid Neoplasm With GLI1 Gene Rearrangements, Frequent S100 Protein Expression, and Metastatic Potential: Expanding the Spectrum of Pathologic Entities With ACTB/MALAT1/PTCH1-GLI1 Fusions.
The American journal of surgical pathology(2018 Jan)
Antonescu CR,Agaram NP,Sung YS,Zhang L,Swanson D,Dickson BC

ROS and glutathionylation balance cytoskeletal dynamics in neutrophil extracellular trap formation.
The Journal of cell biology216(12)4073-4090(2017 Dec)
Stojkov D,Amini P,Oberson K,Sokollik C,Duppenthaler A,Simon HU,Yousefi S

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ACTB cDNA ORF clone, Homo sapiens(human)

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