HACE1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_020771.3	NP_065822.2	E3 ubiquitin-protein ligase HACE1 isoform a
NM_001350558.1	NP_001337487.1	E3 ubiquitin-protein ligase HACE1 isoform d
NM_001350559.1	NP_001337488.1	E3 ubiquitin-protein ligase HACE1 isoform h
NM_001350554.1	NP_001337483.1	E3 ubiquitin-protein ligase HACE1 isoform e
NM_001350560.2	NP_001337489.1	E3 ubiquitin-protein ligase HACE1 isoform i
NM_001350557.2	NP_001337486.1	E3 ubiquitin-protein ligase HACE1 isoform d
NM_001350556.2	NP_001337485.1	E3 ubiquitin-protein ligase HACE1 isoform g
NM_001321083.2	NP_001308012.1	E3 ubiquitin-protein ligase HACE1 isoform c
XM_017011114.2	XP_016866603.1	E3 ubiquitin-protein ligase HACE1 isoform X1
NM_001350555.1	NP_001337484.1	E3 ubiquitin-protein ligase HACE1 isoform f
XM_017011119.1	XP_016866608.1	E3 ubiquitin-protein ligase HACE1 isoform X3
XM_017011119.2	XP_016866608.1	E3 ubiquitin-protein ligase HACE1 isoform X2
NM_001321083.1	NP_001308012.1	E3 ubiquitin-protein ligase HACE1 isoform c
NM_001321080.1	NP_001308009.1	E3 ubiquitin-protein ligase HACE1 isoform b
NM_001350555.2	NP_001337484.1	E3 ubiquitin-protein ligase HACE1 isoform f
NM_020771.4	NP_065822.2	E3 ubiquitin-protein ligase HACE1 isoform a
NM_001350559.2	NP_001337488.1	E3 ubiquitin-protein ligase HACE1 isoform h
NM_001350558.2	NP_001337487.1	E3 ubiquitin-protein ligase HACE1 isoform d
XM_017011114.1	XP_016866603.1	E3 ubiquitin-protein ligase HACE1 isoform X1
NM_001321084.1	NP_001308013.1	E3 ubiquitin-protein ligase HACE1 isoform d
NM_001350556.1	NP_001337485.1	E3 ubiquitin-protein ligase HACE1 isoform g
NM_001321080.2	NP_001308009.1	E3 ubiquitin-protein ligase HACE1 isoform b
NM_001321084.2	NP_001308013.1	E3 ubiquitin-protein ligase HACE1 isoform d
NM_001350554.2	NP_001337483.1	E3 ubiquitin-protein ligase HACE1 isoform e
XM_017011122.1	XP_016866611.1	E3 ubiquitin-protein ligase HACE1 isoform X3
XM_017011118.1	XP_016866607.1	E3 ubiquitin-protein ligase HACE1 isoform X3
NM_001350560.1	NP_001337489.1	E3 ubiquitin-protein ligase HACE1 isoform i
NM_001350557.1	NP_001337486.1	E3 ubiquitin-protein ligase HACE1 isoform d

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17253	NM_020771.4 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu101566	NM_001350560.2 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu40894	NM_001321083.2 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu40895	NM_001321080.2 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu40896	NM_001350557.2 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu40896	NM_001321084.2 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu40896	NM_001350558.2 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu70565	NM_001350556.2 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu70566	NM_001350559.2 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu40896	XM_017011118.1 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu40896	XM_017011119.2 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu70566	XM_017011122.1 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu101567	NM_001350555.2 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu96713	NM_001350554.2 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu96713	XM_017011114.2 Latest version!	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu40896	XM_017011119.1	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu40894	NM_001321083.1	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu101567	NM_001350555.1	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu17253	NM_020771.3	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu70566	NM_001350559.1	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu40896	NM_001350558.1	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu96713	XM_017011114.1	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu70565	NM_001350556.1	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu40895	NM_001321080.1	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu40896	NM_001321084.1	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu96713	NM_001350554.1	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu101566	NM_001350560.1	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu40896	NM_001350557.1	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

HACE1 ( NM_020771.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065822.2 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17253
Clone ID Related Accession (Same CDS sequence)	NM_020771.3 , NM_020771.4
Accession Version	NM_020771.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK131207.1, BC034982.2, BC038119.1 and AJ420474.1. This sequence is a reference standard in the RefSeqGene project. On Oct 31, 2006 this sequence version replaced NM_020771.2. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034982.2, SRR1660807.129811.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC 
TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA 
CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT 
AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACATTG GCTGGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCATG TCAGTGATGT TGATGTTGAG 
GATGCCATGG GGCAGACAGC ACTGCATGTT GCCTGCCAGA ACGGTCACAA GACGACAGTG 
CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCAGG AGCAACTCCA 
TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GATACAGCAC AGATCCTACT ATTACGAGGA 
GCCAAATATC TGCCAGATAA AAATGGAGTA ACTCCTCTGG ATTTATGTGT ACAGGGTGGA 
TATGGAGAGA CTTGTGAAGT ATTAATTCAA TATCACCCGA GGCTTTTTCA GACTATTATT 
CAAATGACAC AGAATGAAGA CCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG 
TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCTACA 
ACAAATGGTC ATAAACTGCT TAGCCTCTCT AGCAATTATG ATGCTCAAAT GAAGAGCCTT 
TTAAGGATTG TGAGAATGTT TTGTCACGTC TTTCGAATTG GTCCATCCTC CCCCAGTAAT 
GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGAA GCCAGGTGTT CAAGCCTCTG 
GAATTGCTTT GGCACTCGTT AGATGAATGG CTAGTTTTAA TAGCCACAGA ATTGATGAAA 
AACAAAAGAG ACTCAACAGA GATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA 
GATGCTGCTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC 
ACTGGCACAA GGGAATCTAA ACCAGATGCT CTTGCAGGGA GACAGGAAGC CAGTGCAGAT 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCGGGA ATGACTTCAC CTCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAC 
CACTTTCACT TTCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA 
GCACAGCCTT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA 
GATTCAGATA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCACAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA 
GGGATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT GCGTGAGTGG 
TTTGATATTC TGTCCAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT 
GATGGAACAA CTTTTCAGCC TAATAGCAAC TCTTATGTAA ATCCTGATCA CTTGAACTAT 
TTTCGGTTTG CTGGGCAGAT CTTGGGATTA GCGTTGAACC ACAGGCAGCT GGTCAATATT 
TACTTCACAC GATCCTTCTA CAAGCACATT CTTGGTATTC CTGTAAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCGAAAAAT TTGCAATGGA TTTTAGATAA TGATATAAGT 
GATCTGGGTC TAGAACTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCTTTGA AACCTGGGGG TGGGAGTATT CTTGTGACAC AAAATAATAA AGCGGAGTAC 
GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCC TCCCTCATAC AGCTTTTTGA TGAATATGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTA 
GAAGACATTA CTCAAGAGGA GAGAGTTCTT CTCTTACAGT TTGTTACGGG CAGTTCCAGG 
GTCCCACATG GTGGGTTTGC TAATATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATC 
GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCAA GCACATGCAT CAACATGCTC 
AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCACTACAT 
TGTGGCAGCT ATGGTTACAC AATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065822.2
CDS	278..3007
Translation	MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_020771.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020771.3

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC 
TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA 
CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT 
AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACATTG GCTGGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCATG TCAGTGATGT TGATGTTGAG 
GATGCCATGG GGCAGACAGC ACTGCATGTT GCCTGCCAGA ACGGTCACAA GACGACAGTG 
CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCAGG AGCAACTCCA 
TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GATACAGCAC AGATCCTACT ATTACGAGGA 
GCCAAATATC TGCCAGATAA AAATGGAGTA ACTCCTCTGG ATTTATGTGT ACAGGGTGGA 
TATGGAGAGA CTTGTGAAGT ATTAATTCAA TATCACCCGA GGCTTTTTCA GACTATTATT 
CAAATGACAC AGAATGAAGA CCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG 
TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCTACA 
ACAAATGGTC ATAAACTGCT TAGCCTCTCT AGCAATTATG ATGCTCAAAT GAAGAGCCTT 
TTAAGGATTG TGAGAATGTT TTGTCACGTC TTTCGAATTG GTCCATCCTC CCCCAGTAAT 
GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGAA GCCAGGTGTT CAAGCCTCTG 
GAATTGCTTT GGCACTCGTT AGATGAATGG CTAGTTTTAA TAGCCACAGA ATTGATGAAA 
AACAAAAGAG ACTCAACAGA GATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA 
GATGCTGCTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC 
ACTGGCACAA GGGAATCTAA ACCAGATGCT CTTGCAGGGA GACAGGAAGC CAGTGCAGAT 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCGGGA ATGACTTCAC CTCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAC 
CACTTTCACT TTCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA 
GCACAGCCTT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA 
GATTCAGATA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCACAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA 
GGGATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT GCGTGAGTGG 
TTTGATATTC TGTCCAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT 
GATGGAACAA CTTTTCAGCC TAATAGCAAC TCTTATGTAA ATCCTGATCA CTTGAACTAT 
TTTCGGTTTG CTGGGCAGAT CTTGGGATTA GCGTTGAACC ACAGGCAGCT GGTCAATATT 
TACTTCACAC GATCCTTCTA CAAGCACATT CTTGGTATTC CTGTAAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCGAAAAAT TTGCAATGGA TTTTAGATAA TGATATAAGT 
GATCTGGGTC TAGAACTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCTTTGA AACCTGGGGG TGGGAGTATT CTTGTGACAC AAAATAATAA AGCGGAGTAC 
GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCC TCCCTCATAC AGCTTTTTGA TGAATATGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTA 
GAAGACATTA CTCAAGAGGA GAGAGTTCTT CTCTTACAGT TTGTTACGGG CAGTTCCAGG 
GTCCCACATG GTGGGTTTGC TAATATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATC 
GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCAA GCACATGCAT CAACATGCTC 
AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCACTACAT 
TGTGGCAGCT ATGGTTACAC AATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_020771.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065822.2 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17253
Clone ID Related Accession (Same CDS sequence)	NM_020771.3 , NM_020771.4
Accession Version	NM_020771.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK131207.1, BC034982.2, BC038119.1 and AJ420474.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_020771.3. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.248387.1, SRR1660807.129811.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262903.9/ ENSP00000262903.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC 
TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA 
CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT 
AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACATTG GCTGGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCATG TCAGTGATGT TGATGTTGAG 
GATGCCATGG GGCAGACAGC ACTGCATGTT GCCTGCCAGA ACGGTCACAA GACGACAGTG 
CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCAGG AGCAACTCCA 
TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GATACAGCAC AGATCCTACT ATTACGAGGA 
GCCAAATATC TGCCAGATAA AAATGGAGTA ACTCCTCTGG ATTTATGTGT ACAGGGTGGA 
TATGGAGAGA CTTGTGAAGT ATTAATTCAA TATCACCCGA GGCTTTTTCA GACTATTATT 
CAAATGACAC AGAATGAAGA CCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG 
TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCTACA 
ACAAATGGTC ATAAACTGCT TAGCCTCTCT AGCAATTATG ATGCTCAAAT GAAGAGCCTT 
TTAAGGATTG TGAGAATGTT TTGTCACGTC TTTCGAATTG GTCCATCCTC CCCCAGTAAT 
GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGAA GCCAGGTGTT CAAGCCTCTG 
GAATTGCTTT GGCACTCGTT AGATGAATGG CTAGTTTTAA TAGCCACAGA ATTGATGAAA 
AACAAAAGAG ACTCAACAGA GATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA 
GATGCTGCTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC 
ACTGGCACAA GGGAATCTAA ACCAGATGCT CTTGCAGGGA GACAGGAAGC CAGTGCAGAT 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCGGGA ATGACTTCAC CTCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAC 
CACTTTCACT TTCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA 
GCACAGCCTT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA 
GATTCAGATA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCACAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA 
GGGATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT GCGTGAGTGG 
TTTGATATTC TGTCCAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT 
GATGGAACAA CTTTTCAGCC TAATAGCAAC TCTTATGTAA ATCCTGATCA CTTGAACTAT 
TTTCGGTTTG CTGGGCAGAT CTTGGGATTA GCGTTGAACC ACAGGCAGCT GGTCAATATT 
TACTTCACAC GATCCTTCTA CAAGCACATT CTTGGTATTC CTGTAAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCGAAAAAT TTGCAATGGA TTTTAGATAA TGATATAAGT 
GATCTGGGTC TAGAACTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCTTTGA AACCTGGGGG TGGGAGTATT CTTGTGACAC AAAATAATAA AGCGGAGTAC 
GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCC TCCCTCATAC AGCTTTTTGA TGAATATGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTA 
GAAGACATTA CTCAAGAGGA GAGAGTTCTT CTCTTACAGT TTGTTACGGG CAGTTCCAGG 
GTCCCACATG GTGGGTTTGC TAATATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATC 
GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCAA GCACATGCAT CAACATGCTC 
AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCACTACAT 
TGTGGCAGCT ATGGTTACAC AATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065822.2
CDS	278..3007
Translation	MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_020771.4
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020771.4

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC 
TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA 
CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT 
AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACATTG GCTGGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCATG TCAGTGATGT TGATGTTGAG 
GATGCCATGG GGCAGACAGC ACTGCATGTT GCCTGCCAGA ACGGTCACAA GACGACAGTG 
CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCAGG AGCAACTCCA 
TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GATACAGCAC AGATCCTACT ATTACGAGGA 
GCCAAATATC TGCCAGATAA AAATGGAGTA ACTCCTCTGG ATTTATGTGT ACAGGGTGGA 
TATGGAGAGA CTTGTGAAGT ATTAATTCAA TATCACCCGA GGCTTTTTCA GACTATTATT 
CAAATGACAC AGAATGAAGA CCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAGCATTTG 
TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCTACA 
ACAAATGGTC ATAAACTGCT TAGCCTCTCT AGCAATTATG ATGCTCAAAT GAAGAGCCTT 
TTAAGGATTG TGAGAATGTT TTGTCACGTC TTTCGAATTG GTCCATCCTC CCCCAGTAAT 
GGAATTGATA TGGGCTACAA TGGGAATAAA ACTCCAAGAA GCCAGGTGTT CAAGCCTCTG 
GAATTGCTTT GGCACTCGTT AGATGAATGG CTAGTTTTAA TAGCCACAGA ATTGATGAAA 
AACAAAAGAG ACTCAACAGA GATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA 
GATGCTGCTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTGTCC 
ACTGGCACAA GGGAATCTAA ACCAGATGCT CTTGCAGGGA GACAGGAAGC CAGTGCAGAT 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCGGGA ATGACTTCAC CTCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAC 
CACTTTCACT TTCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA 
GCACAGCCTT TTAAAGATCG CTGTGAATGG TTCTATGAAC ATTTGCATTC AGGACAGCCA 
GATTCAGATA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTGGT TCACAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ATTGTGCAAA GCTAAAGCAA 
GGGATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGTC AAGGTGTTGT GCGTGAGTGG 
TTTGATATTC TGTCCAATGA GATAGTCAAT CCTGATTATG CATTGTTTAC CCAGTCAGCT 
GATGGAACAA CTTTTCAGCC TAATAGCAAC TCTTATGTAA ATCCTGATCA CTTGAACTAT 
TTTCGGTTTG CTGGGCAGAT CTTGGGATTA GCGTTGAACC ACAGGCAGCT GGTCAATATT 
TACTTCACAC GATCCTTCTA CAAGCACATT CTTGGTATTC CTGTAAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCGAAAAAT TTGCAATGGA TTTTAGATAA TGATATAAGT 
GATCTGGGTC TAGAACTAAC TTTTTCTGTT GAGACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCTTTGA AACCTGGGGG TGGGAGTATT CTTGTGACAC AAAATAATAA AGCGGAGTAC 
GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AGCCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCC TCCCTCATAC AGCTTTTTGA TGAATATGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GTGATTGGAT AAAAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTA 
GAAGACATTA CTCAAGAGGA GAGAGTTCTT CTCTTACAGT TTGTTACGGG CAGTTCCAGG 
GTCCCACATG GTGGGTTTGC TAATATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATC 
GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCAA GCACATGCAT CAACATGCTC 
AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCACTACAT 
TGTGGCAGCT ATGGTTACAC AATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001350560.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337489.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 12, mRNA.

Price & Availability↑

CloneID	OHu101566
Clone ID Related Accession (Same CDS sequence)	NM_001350560.2 , NM_001350560.1
Accession Version	NM_001350560.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On Jun 1, 2019 this sequence version replaced NM_001350560.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.273495.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACACAGA ATGAAGACCT CCGAGAAAAC ATGTTACGGC AAGTTCTGGA GCATTTGTCT 
CAGCAAAGTG AAAGCCAGTA CCTAAAGATT CTAACAAGCC TTGCTGAAGT TGCTACAACA 
AATGGTCATA AACTGCTTAG CCTCTCTAGC AATTATGATG CTCAAATGAA GAGCCTTTTA 
AGGATTGTGA GAATGTTTTG TCACGTCTTT CGAATTGGTC CATCCTCCCC CAGTAATGGA 
ATTGATATGG GCTACAATGG GAATAAAACT CCAAGAAGCC AGGTGTTCAA GCCTCTGGAA 
TTGCTTTGGC ACTCGTTAGA TGAATGGCTA GTTTTAATAG CCACAGAATT GATGAAAAAC 
AAAAGAGACT CAACAGAGAT CACTTCTATT TTACTGAAAC AAAAAGGCCA AGATCAAGAT 
GCTGCTTCCA TTCCTCCATT TGAACCTCCA GGACCTGGGA GCTATGAAAA TCTGTCCACT 
GGCACAAGGG AATCTAAACC AGATGCTCTT GCAGGGAGAC AGGAAGCCAG TGCAGATTGT 
CAGGATGTTA TTTCTATGAC AGCTAACCGG CTAAGTGCTG TCATTCAAGC TTTTTACATG 
TGCTGTTCTT GTCAGATGCC TCCGGGAATG ACTTCACCTC GTTTCATTGA ATTTGTCTGC 
AAACATGATG AAGTTTTAAA ATGCTTTGTT AATAGAAATC CCAAAATTAT ATTTGACCAC 
TTTCACTTTC TCCTTGAATG TCCTGAGTTG ATGTCAAGAT TCATGCATAT CATAAAAGCA 
CAGCCTTTTA AAGATCGCTG TGAATGGTTC TATGAACATT TGCATTCAGG ACAGCCAGAT 
TCAGATATGG TGCACAGGCC AGTGAATGAA AATGATATCC TGCTGGTTCA CAGAGATTCT 
ATTTTTAGGA GTAGCTGTGA AGTTGTGTCA AAAGCAAATT GTGCAAAGCT AAAGCAAGGG 
ATTGCTGTAC GGTTCCATGG AGAAGAAGGC ATGGGTCAAG GTGTTGTGCG TGAGTGGTTT 
GATATTCTGT CCAATGAGAT AGTCAATCCT GATTATGCAT TGTTTACCCA GTCAGCTGAT 
GGAACAACTT TTCAGCCTAA TAGCAACTCT TATGTAAATC CTGATCACTT GAACTATTTT 
CGGTTTGCTG GGCAGATCTT GGGATTAGCG TTGAACCACA GGCAGCTGGT CAATATTTAC 
TTCACACGAT CCTTCTACAA GCACATTCTT GGTATTCCTG TAAATTACCA AGATGTGGCA 
TCCATTGATC CAGAATATGC GAAAAATTTG CAATGGATTT TAGATAATGA TATAAGTGAT 
CTGGGTCTAG AACTAACTTT TTCTGTTGAG ACTGATGTGT TTGGAGCAAT GGAAGAGGTG 
CCTTTGAAAC CTGGGGGTGG GAGTATTCTT GTGACACAAA ATAATAAAGC GGAGTACGTC 
CAGCTTGTTA CTGAACTTCG AATGACAAGA GCCATTCAGC CTCAGATCAA TGCTTTTTTA 
CAGGGCTTTC ATATGTTCAT TCCACCCTCC CTCATACAGC TTTTTGATGA ATATGAATTG 
GAGCTACTGC TTTCTGGCAT GCCAGAAATT GATGTGAGTG ATTGGATAAA AAATACAGAA 
TACACAAGTG GCTATGAAAG AGAAGATCCA GTTATTCAGT GGTTCTGGGA AGTTGTAGAA 
GACATTACTC AAGAGGAGAG AGTTCTTCTC TTACAGTTTG TTACGGGCAG TTCCAGGGTC 
CCACATGGTG GGTTTGCTAA TATCATGGGT GGAAGTGGAT TGCAAAACTT TACAATCGCT 
GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCAAGCA CATGCATCAA CATGCTCAAG 
TTACCTGAAT ACCCAAGTAA AGAAATACTC AAGGACAGAC TTCTTGTGGC ACTACATTGT 
GGCAGCTATG GTTACACAAT GGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337489.1
CDS	853..2799
Translation	MTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001350560.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350560.2

ORF Insert Sequence:

ATGACACAGA ATGAAGACCT CCGAGAAAAC ATGTTACGGC AAGTTCTGGA GCATTTGTCT 
CAGCAAAGTG AAAGCCAGTA CCTAAAGATT CTAACAAGCC TTGCTGAAGT TGCTACAACA 
AATGGTCATA AACTGCTTAG CCTCTCTAGC AATTATGATG CTCAAATGAA GAGCCTTTTA 
AGGATTGTGA GAATGTTTTG TCACGTCTTT CGAATTGGTC CATCCTCCCC CAGTAATGGA 
ATTGATATGG GCTACAATGG GAATAAAACT CCAAGAAGCC AGGTGTTCAA GCCTCTGGAA 
TTGCTTTGGC ACTCGTTAGA TGAATGGCTA GTTTTAATAG CCACAGAATT GATGAAAAAC 
AAAAGAGACT CAACAGAGAT CACTTCTATT TTACTGAAAC AAAAAGGCCA AGATCAAGAT 
GCTGCTTCCA TTCCTCCATT TGAACCTCCA GGACCTGGGA GCTATGAAAA TCTGTCCACT 
GGCACAAGGG AATCTAAACC AGATGCTCTT GCAGGGAGAC AGGAAGCCAG TGCAGATTGT 
CAGGATGTTA TTTCTATGAC AGCTAACCGG CTAAGTGCTG TCATTCAAGC TTTTTACATG 
TGCTGTTCTT GTCAGATGCC TCCGGGAATG ACTTCACCTC GTTTCATTGA ATTTGTCTGC 
AAACATGATG AAGTTTTAAA ATGCTTTGTT AATAGAAATC CCAAAATTAT ATTTGACCAC 
TTTCACTTTC TCCTTGAATG TCCTGAGTTG ATGTCAAGAT TCATGCATAT CATAAAAGCA 
CAGCCTTTTA AAGATCGCTG TGAATGGTTC TATGAACATT TGCATTCAGG ACAGCCAGAT 
TCAGATATGG TGCACAGGCC AGTGAATGAA AATGATATCC TGCTGGTTCA CAGAGATTCT 
ATTTTTAGGA GTAGCTGTGA AGTTGTGTCA AAAGCAAATT GTGCAAAGCT AAAGCAAGGG 
ATTGCTGTAC GGTTCCATGG AGAAGAAGGC ATGGGTCAAG GTGTTGTGCG TGAGTGGTTT 
GATATTCTGT CCAATGAGAT AGTCAATCCT GATTATGCAT TGTTTACCCA GTCAGCTGAT 
GGAACAACTT TTCAGCCTAA TAGCAACTCT TATGTAAATC CTGATCACTT GAACTATTTT 
CGGTTTGCTG GGCAGATCTT GGGATTAGCG TTGAACCACA GGCAGCTGGT CAATATTTAC 
TTCACACGAT CCTTCTACAA GCACATTCTT GGTATTCCTG TAAATTACCA AGATGTGGCA 
TCCATTGATC CAGAATATGC GAAAAATTTG CAATGGATTT TAGATAATGA TATAAGTGAT 
CTGGGTCTAG AACTAACTTT TTCTGTTGAG ACTGATGTGT TTGGAGCAAT GGAAGAGGTG 
CCTTTGAAAC CTGGGGGTGG GAGTATTCTT GTGACACAAA ATAATAAAGC GGAGTACGTC 
CAGCTTGTTA CTGAACTTCG AATGACAAGA GCCATTCAGC CTCAGATCAA TGCTTTTTTA 
CAGGGCTTTC ATATGTTCAT TCCACCCTCC CTCATACAGC TTTTTGATGA ATATGAATTG 
GAGCTACTGC TTTCTGGCAT GCCAGAAATT GATGTGAGTG ATTGGATAAA AAATACAGAA 
TACACAAGTG GCTATGAAAG AGAAGATCCA GTTATTCAGT GGTTCTGGGA AGTTGTAGAA 
GACATTACTC AAGAGGAGAG AGTTCTTCTC TTACAGTTTG TTACGGGCAG TTCCAGGGTC 
CCACATGGTG GGTTTGCTAA TATCATGGGT GGAAGTGGAT TGCAAAACTT TACAATCGCT 
GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCAAGCA CATGCATCAA CATGCTCAAG 
TTACCTGAAT ACCCAAGTAA AGAAATACTC AAGGACAGAC TTCTTGTGGC ACTACATTGT 
GGCAGCTATG GTTACACAAT GGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001350560.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337489.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 12, mRNA.

Price & Availability↑

CloneID	OHu101566
Clone ID Related Accession (Same CDS sequence)	NM_001350560.2 , NM_001350560.1
Accession Version	NM_001350560.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.273495.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACACAGA ATGAAGACCT CCGAGAAAAC ATGTTACGGC AAGTTCTGGA GCATTTGTCT 
CAGCAAAGTG AAAGCCAGTA CCTAAAGATT CTAACAAGCC TTGCTGAAGT TGCTACAACA 
AATGGTCATA AACTGCTTAG CCTCTCTAGC AATTATGATG CTCAAATGAA GAGCCTTTTA 
AGGATTGTGA GAATGTTTTG TCACGTCTTT CGAATTGGTC CATCCTCCCC CAGTAATGGA 
ATTGATATGG GCTACAATGG GAATAAAACT CCAAGAAGCC AGGTGTTCAA GCCTCTGGAA 
TTGCTTTGGC ACTCGTTAGA TGAATGGCTA GTTTTAATAG CCACAGAATT GATGAAAAAC 
AAAAGAGACT CAACAGAGAT CACTTCTATT TTACTGAAAC AAAAAGGCCA AGATCAAGAT 
GCTGCTTCCA TTCCTCCATT TGAACCTCCA GGACCTGGGA GCTATGAAAA TCTGTCCACT 
GGCACAAGGG AATCTAAACC AGATGCTCTT GCAGGGAGAC AGGAAGCCAG TGCAGATTGT 
CAGGATGTTA TTTCTATGAC AGCTAACCGG CTAAGTGCTG TCATTCAAGC TTTTTACATG 
TGCTGTTCTT GTCAGATGCC TCCGGGAATG ACTTCACCTC GTTTCATTGA ATTTGTCTGC 
AAACATGATG AAGTTTTAAA ATGCTTTGTT AATAGAAATC CCAAAATTAT ATTTGACCAC 
TTTCACTTTC TCCTTGAATG TCCTGAGTTG ATGTCAAGAT TCATGCATAT CATAAAAGCA 
CAGCCTTTTA AAGATCGCTG TGAATGGTTC TATGAACATT TGCATTCAGG ACAGCCAGAT 
TCAGATATGG TGCACAGGCC AGTGAATGAA AATGATATCC TGCTGGTTCA CAGAGATTCT 
ATTTTTAGGA GTAGCTGTGA AGTTGTGTCA AAAGCAAATT GTGCAAAGCT AAAGCAAGGG 
ATTGCTGTAC GGTTCCATGG AGAAGAAGGC ATGGGTCAAG GTGTTGTGCG TGAGTGGTTT 
GATATTCTGT CCAATGAGAT AGTCAATCCT GATTATGCAT TGTTTACCCA GTCAGCTGAT 
GGAACAACTT TTCAGCCTAA TAGCAACTCT TATGTAAATC CTGATCACTT GAACTATTTT 
CGGTTTGCTG GGCAGATCTT GGGATTAGCG TTGAACCACA GGCAGCTGGT CAATATTTAC 
TTCACACGAT CCTTCTACAA GCACATTCTT GGTATTCCTG TAAATTACCA AGATGTGGCA 
TCCATTGATC CAGAATATGC GAAAAATTTG CAATGGATTT TAGATAATGA TATAAGTGAT 
CTGGGTCTAG AACTAACTTT TTCTGTTGAG ACTGATGTGT TTGGAGCAAT GGAAGAGGTG 
CCTTTGAAAC CTGGGGGTGG GAGTATTCTT GTGACACAAA ATAATAAAGC GGAGTACGTC 
CAGCTTGTTA CTGAACTTCG AATGACAAGA GCCATTCAGC CTCAGATCAA TGCTTTTTTA 
CAGGGCTTTC ATATGTTCAT TCCACCCTCC CTCATACAGC TTTTTGATGA ATATGAATTG 
GAGCTACTGC TTTCTGGCAT GCCAGAAATT GATGTGAGTG ATTGGATAAA AAATACAGAA 
TACACAAGTG GCTATGAAAG AGAAGATCCA GTTATTCAGT GGTTCTGGGA AGTTGTAGAA 
GACATTACTC AAGAGGAGAG AGTTCTTCTC TTACAGTTTG TTACGGGCAG TTCCAGGGTC 
CCACATGGTG GGTTTGCTAA TATCATGGGT GGAAGTGGAT TGCAAAACTT TACAATCGCT 
GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCAAGCA CATGCATCAA CATGCTCAAG 
TTACCTGAAT ACCCAAGTAA AGAAATACTC AAGGACAGAC TTCTTGTGGC ACTACATTGT 
GGCAGCTATG GTTACACAAT GGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337489.1
CDS	853..2799
Translation	MTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001350560.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350560.1

ORF Insert Sequence:

ATGACACAGA ATGAAGACCT CCGAGAAAAC ATGTTACGGC AAGTTCTGGA GCATTTGTCT 
CAGCAAAGTG AAAGCCAGTA CCTAAAGATT CTAACAAGCC TTGCTGAAGT TGCTACAACA 
AATGGTCATA AACTGCTTAG CCTCTCTAGC AATTATGATG CTCAAATGAA GAGCCTTTTA 
AGGATTGTGA GAATGTTTTG TCACGTCTTT CGAATTGGTC CATCCTCCCC CAGTAATGGA 
ATTGATATGG GCTACAATGG GAATAAAACT CCAAGAAGCC AGGTGTTCAA GCCTCTGGAA 
TTGCTTTGGC ACTCGTTAGA TGAATGGCTA GTTTTAATAG CCACAGAATT GATGAAAAAC 
AAAAGAGACT CAACAGAGAT CACTTCTATT TTACTGAAAC AAAAAGGCCA AGATCAAGAT 
GCTGCTTCCA TTCCTCCATT TGAACCTCCA GGACCTGGGA GCTATGAAAA TCTGTCCACT 
GGCACAAGGG AATCTAAACC AGATGCTCTT GCAGGGAGAC AGGAAGCCAG TGCAGATTGT 
CAGGATGTTA TTTCTATGAC AGCTAACCGG CTAAGTGCTG TCATTCAAGC TTTTTACATG 
TGCTGTTCTT GTCAGATGCC TCCGGGAATG ACTTCACCTC GTTTCATTGA ATTTGTCTGC 
AAACATGATG AAGTTTTAAA ATGCTTTGTT AATAGAAATC CCAAAATTAT ATTTGACCAC 
TTTCACTTTC TCCTTGAATG TCCTGAGTTG ATGTCAAGAT TCATGCATAT CATAAAAGCA 
CAGCCTTTTA AAGATCGCTG TGAATGGTTC TATGAACATT TGCATTCAGG ACAGCCAGAT 
TCAGATATGG TGCACAGGCC AGTGAATGAA AATGATATCC TGCTGGTTCA CAGAGATTCT 
ATTTTTAGGA GTAGCTGTGA AGTTGTGTCA AAAGCAAATT GTGCAAAGCT AAAGCAAGGG 
ATTGCTGTAC GGTTCCATGG AGAAGAAGGC ATGGGTCAAG GTGTTGTGCG TGAGTGGTTT 
GATATTCTGT CCAATGAGAT AGTCAATCCT GATTATGCAT TGTTTACCCA GTCAGCTGAT 
GGAACAACTT TTCAGCCTAA TAGCAACTCT TATGTAAATC CTGATCACTT GAACTATTTT 
CGGTTTGCTG GGCAGATCTT GGGATTAGCG TTGAACCACA GGCAGCTGGT CAATATTTAC 
TTCACACGAT CCTTCTACAA GCACATTCTT GGTATTCCTG TAAATTACCA AGATGTGGCA 
TCCATTGATC CAGAATATGC GAAAAATTTG CAATGGATTT TAGATAATGA TATAAGTGAT 
CTGGGTCTAG AACTAACTTT TTCTGTTGAG ACTGATGTGT TTGGAGCAAT GGAAGAGGTG 
CCTTTGAAAC CTGGGGGTGG GAGTATTCTT GTGACACAAA ATAATAAAGC GGAGTACGTC 
CAGCTTGTTA CTGAACTTCG AATGACAAGA GCCATTCAGC CTCAGATCAA TGCTTTTTTA 
CAGGGCTTTC ATATGTTCAT TCCACCCTCC CTCATACAGC TTTTTGATGA ATATGAATTG 
GAGCTACTGC TTTCTGGCAT GCCAGAAATT GATGTGAGTG ATTGGATAAA AAATACAGAA 
TACACAAGTG GCTATGAAAG AGAAGATCCA GTTATTCAGT GGTTCTGGGA AGTTGTAGAA 
GACATTACTC AAGAGGAGAG AGTTCTTCTC TTACAGTTTG TTACGGGCAG TTCCAGGGTC 
CCACATGGTG GGTTTGCTAA TATCATGGGT GGAAGTGGAT TGCAAAACTT TACAATCGCT 
GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCAAGCA CATGCATCAA CATGCTCAAG 
TTACCTGAAT ACCCAAGTAA AGAAATACTC AAGGACAGAC TTCTTGTGGC ACTACATTGT 
GGCAGCTATG GTTACACAAT GGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001321083.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308012.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu40894
Clone ID Related Accession (Same CDS sequence)	NM_001321083.2 , NM_001321083.1
Accession Version	NM_001321083.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB094058.1, CN288032.1, BC034982.2, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001321083.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.42112.1, SRR1803614.223452.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG 
AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA 
ATACATTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT GCAGCATGTC 
AGTGATGTTG ATGTTGAGGA TGCCATGGGG CAGACAGCAC TGCATGTTGC CTGCCAGAAC 
GGTCACAAGA CGACAGTGCA GTGCTTGCTA GACAGTGGTG CTGATATTAA CAGGCCAAAT 
GTATCAGGAG CAACTCCATT GTACTTTGCT TGCAGTCATG GTCAGAGAGA TACAGCACAG 
ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT 
TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG 
CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT 
GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT 
CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC 
CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA 
GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA 
CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA 
CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT 
CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA 
TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT 
TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT 
TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA 
GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA 
TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT 
CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC 
AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT 
GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT 
TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA 
AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG 
CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT 
GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT 
GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA 
TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC 
ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA 
CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308012.1
CDS	195..2822
Translation	MPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001321083.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321083.2

ORF Insert Sequence:

ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG 
AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA 
ATACATTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT GCAGCATGTC 
AGTGATGTTG ATGTTGAGGA TGCCATGGGG CAGACAGCAC TGCATGTTGC CTGCCAGAAC 
GGTCACAAGA CGACAGTGCA GTGCTTGCTA GACAGTGGTG CTGATATTAA CAGGCCAAAT 
GTATCAGGAG CAACTCCATT GTACTTTGCT TGCAGTCATG GTCAGAGAGA TACAGCACAG 
ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT 
TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG 
CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT 
GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT 
CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC 
CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA 
GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA 
CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA 
CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT 
CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA 
TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT 
TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT 
TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA 
GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA 
TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT 
CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC 
AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT 
GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT 
TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA 
AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG 
CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT 
GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT 
GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA 
TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC 
ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA 
CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001321083.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308012.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu40894
Clone ID Related Accession (Same CDS sequence)	NM_001321083.2 , NM_001321083.1
Accession Version	NM_001321083.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB094058.1, CN288032.1, BC034982.2, AL513472.9 and AL590402.10. On Mar 18, 2016 this sequence version replaced XM_006715528.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.42112.1, SRR1803614.223452.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG 
AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA 
ATACATTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT GCAGCATGTC 
AGTGATGTTG ATGTTGAGGA TGCCATGGGG CAGACAGCAC TGCATGTTGC CTGCCAGAAC 
GGTCACAAGA CGACAGTGCA GTGCTTGCTA GACAGTGGTG CTGATATTAA CAGGCCAAAT 
GTATCAGGAG CAACTCCATT GTACTTTGCT TGCAGTCATG GTCAGAGAGA TACAGCACAG 
ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT 
TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG 
CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT 
GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT 
CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC 
CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA 
GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA 
CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA 
CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT 
CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA 
TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT 
TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT 
TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA 
GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA 
TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT 
CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC 
AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT 
GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT 
TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA 
AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG 
CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT 
GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT 
GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA 
TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC 
ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA 
CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308012.1
CDS	195..2822
Translation	MPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001321083.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321083.1

ORF Insert Sequence:

ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG 
AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA 
ATACATTGGC TGGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT GCAGCATGTC 
AGTGATGTTG ATGTTGAGGA TGCCATGGGG CAGACAGCAC TGCATGTTGC CTGCCAGAAC 
GGTCACAAGA CGACAGTGCA GTGCTTGCTA GACAGTGGTG CTGATATTAA CAGGCCAAAT 
GTATCAGGAG CAACTCCATT GTACTTTGCT TGCAGTCATG GTCAGAGAGA TACAGCACAG 
ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT 
TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG 
CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT 
GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT 
CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC 
CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA 
GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA 
CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA 
CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT 
CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA 
TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT 
TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT 
TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA 
GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA 
TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT 
CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC 
AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT 
GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT 
TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA 
AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG 
CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT 
GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT 
GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA 
TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC 
ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA 
CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001321080.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308009.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu40895
Clone ID Related Accession (Same CDS sequence)	NM_001321080.1 , NM_001321080.2
Accession Version	NM_001321080.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK131207.1, BC034982.2, AL513472.9 and AL590402.10. On Mar 18, 2016 this sequence version replaced XM_006715529.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.183999.1, SRR1660807.22692.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC 
TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA 
CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT 
AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA 
GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCAGGAG CAACTCCATT GTACTTTGCT 
TGCAGTCATG GTCAGAGAGA TACAGCACAG ATCCTACTAT TACGAGGAGC CAAATATCTG 
CCAGATAAAA ATGGAGTAAC TCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACT 
TGTGAAGTAT TAATTCAATA TCACCCGAGG CTTTTTCAGA CTATTATTCA AATGACACAG 
AATGAAGACC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT 
GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCTACAAC AAATGGTCAT 
AAACTGCTTA GCCTCTCTAG CAATTATGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG 
AGAATGTTTT GTCACGTCTT TCGAATTGGT CCATCCTCCC CCAGTAATGG AATTGATATG 
GGCTACAATG GGAATAAAAC TCCAAGAAGC CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG 
CACTCGTTAG ATGAATGGCT AGTTTTAATA GCCACAGAAT TGATGAAAAA CAAAAGAGAC 
TCAACAGAGA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGCTGCTTCC 
ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAAGG 
GAATCTAAAC CAGATGCTCT TGCAGGGAGA CAGGAAGCCA GTGCAGATTG TCAGGATGTT 
ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT 
TGTCAGATGC CTCCGGGAAT GACTTCACCT CGTTTCATTG AATTTGTCTG CAAACATGAT 
GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGACCA CTTTCACTTT 
CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCTTTT 
AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGATATG 
GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ACAGAGATTC TATTTTTAGG 
AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG GATTGCTGTA 
CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG 
TCCAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT 
TTTCAGCCTA ATAGCAACTC TTATGTAAAT CCTGATCACT TGAACTATTT TCGGTTTGCT 
GGGCAGATCT TGGGATTAGC GTTGAACCAC AGGCAGCTGG TCAATATTTA CTTCACACGA 
TCCTTCTACA AGCACATTCT TGGTATTCCT GTAAATTACC AAGATGTGGC ATCCATTGAT 
CCAGAATATG CGAAAAATTT GCAATGGATT TTAGATAATG ATATAAGTGA TCTGGGTCTA 
GAACTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA 
CCTGGGGGTG GGAGTATTCT TGTGACACAA AATAATAAAG CGGAGTACGT CCAGCTTGTT 
ACTGAACTTC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT 
CATATGTTCA TTCCACCCTC CCTCATACAG CTTTTTGATG AATATGAATT GGAGCTACTG 
CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT 
GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTAGA AGACATTACT 
CAAGAGGAGA GAGTTCTTCT CTTACAGTTT GTTACGGGCA GTTCCAGGGT CCCACATGGT 
GGGTTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATCGC TGCTGTGCCA 
TATACTCCAA ATCTTTTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA 
TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CACTACATTG TGGCAGCTAT 
GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308009.1
CDS	278..2875
Translation	MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTATVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001321080.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321080.1

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC 
TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA 
CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT 
AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA 
GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCAGGAG CAACTCCATT GTACTTTGCT 
TGCAGTCATG GTCAGAGAGA TACAGCACAG ATCCTACTAT TACGAGGAGC CAAATATCTG 
CCAGATAAAA ATGGAGTAAC TCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACT 
TGTGAAGTAT TAATTCAATA TCACCCGAGG CTTTTTCAGA CTATTATTCA AATGACACAG 
AATGAAGACC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT 
GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCTACAAC AAATGGTCAT 
AAACTGCTTA GCCTCTCTAG CAATTATGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG 
AGAATGTTTT GTCACGTCTT TCGAATTGGT CCATCCTCCC CCAGTAATGG AATTGATATG 
GGCTACAATG GGAATAAAAC TCCAAGAAGC CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG 
CACTCGTTAG ATGAATGGCT AGTTTTAATA GCCACAGAAT TGATGAAAAA CAAAAGAGAC 
TCAACAGAGA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGCTGCTTCC 
ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAAGG 
GAATCTAAAC CAGATGCTCT TGCAGGGAGA CAGGAAGCCA GTGCAGATTG TCAGGATGTT 
ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT 
TGTCAGATGC CTCCGGGAAT GACTTCACCT CGTTTCATTG AATTTGTCTG CAAACATGAT 
GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGACCA CTTTCACTTT 
CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCTTTT 
AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGATATG 
GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ACAGAGATTC TATTTTTAGG 
AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG GATTGCTGTA 
CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG 
TCCAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT 
TTTCAGCCTA ATAGCAACTC TTATGTAAAT CCTGATCACT TGAACTATTT TCGGTTTGCT 
GGGCAGATCT TGGGATTAGC GTTGAACCAC AGGCAGCTGG TCAATATTTA CTTCACACGA 
TCCTTCTACA AGCACATTCT TGGTATTCCT GTAAATTACC AAGATGTGGC ATCCATTGAT 
CCAGAATATG CGAAAAATTT GCAATGGATT TTAGATAATG ATATAAGTGA TCTGGGTCTA 
GAACTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA 
CCTGGGGGTG GGAGTATTCT TGTGACACAA AATAATAAAG CGGAGTACGT CCAGCTTGTT 
ACTGAACTTC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT 
CATATGTTCA TTCCACCCTC CCTCATACAG CTTTTTGATG AATATGAATT GGAGCTACTG 
CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT 
GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTAGA AGACATTACT 
CAAGAGGAGA GAGTTCTTCT CTTACAGTTT GTTACGGGCA GTTCCAGGGT CCCACATGGT 
GGGTTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATCGC TGCTGTGCCA 
TATACTCCAA ATCTTTTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA 
TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CACTACATTG TGGCAGCTAT 
GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001321080.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308009.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu40895
Clone ID Related Accession (Same CDS sequence)	NM_001321080.1 , NM_001321080.2
Accession Version	NM_001321080.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK131207.1, BC034982.2, AL513472.9 and AL590402.10. On Jun 1, 2019 this sequence version replaced NM_001321080.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.22692.1, SRR1803617.253935.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC 
TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA 
CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT 
AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA 
GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCAGGAG CAACTCCATT GTACTTTGCT 
TGCAGTCATG GTCAGAGAGA TACAGCACAG ATCCTACTAT TACGAGGAGC CAAATATCTG 
CCAGATAAAA ATGGAGTAAC TCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACT 
TGTGAAGTAT TAATTCAATA TCACCCGAGG CTTTTTCAGA CTATTATTCA AATGACACAG 
AATGAAGACC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT 
GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCTACAAC AAATGGTCAT 
AAACTGCTTA GCCTCTCTAG CAATTATGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG 
AGAATGTTTT GTCACGTCTT TCGAATTGGT CCATCCTCCC CCAGTAATGG AATTGATATG 
GGCTACAATG GGAATAAAAC TCCAAGAAGC CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG 
CACTCGTTAG ATGAATGGCT AGTTTTAATA GCCACAGAAT TGATGAAAAA CAAAAGAGAC 
TCAACAGAGA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGCTGCTTCC 
ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAAGG 
GAATCTAAAC CAGATGCTCT TGCAGGGAGA CAGGAAGCCA GTGCAGATTG TCAGGATGTT 
ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT 
TGTCAGATGC CTCCGGGAAT GACTTCACCT CGTTTCATTG AATTTGTCTG CAAACATGAT 
GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGACCA CTTTCACTTT 
CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCTTTT 
AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGATATG 
GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ACAGAGATTC TATTTTTAGG 
AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG GATTGCTGTA 
CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG 
TCCAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT 
TTTCAGCCTA ATAGCAACTC TTATGTAAAT CCTGATCACT TGAACTATTT TCGGTTTGCT 
GGGCAGATCT TGGGATTAGC GTTGAACCAC AGGCAGCTGG TCAATATTTA CTTCACACGA 
TCCTTCTACA AGCACATTCT TGGTATTCCT GTAAATTACC AAGATGTGGC ATCCATTGAT 
CCAGAATATG CGAAAAATTT GCAATGGATT TTAGATAATG ATATAAGTGA TCTGGGTCTA 
GAACTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA 
CCTGGGGGTG GGAGTATTCT TGTGACACAA AATAATAAAG CGGAGTACGT CCAGCTTGTT 
ACTGAACTTC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT 
CATATGTTCA TTCCACCCTC CCTCATACAG CTTTTTGATG AATATGAATT GGAGCTACTG 
CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT 
GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTAGA AGACATTACT 
CAAGAGGAGA GAGTTCTTCT CTTACAGTTT GTTACGGGCA GTTCCAGGGT CCCACATGGT 
GGGTTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATCGC TGCTGTGCCA 
TATACTCCAA ATCTTTTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA 
TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CACTACATTG TGGCAGCTAT 
GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308009.1
CDS	278..2875
Translation	MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTATVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001321080.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321080.2

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC 
TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA 
CCCCTTCATT TGGCAGCAAG AAATGGGCAG AAGAAATGTA TGAGTAAATT ATTAGAATAT 
AGCGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA 
GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCAGGAG CAACTCCATT GTACTTTGCT 
TGCAGTCATG GTCAGAGAGA TACAGCACAG ATCCTACTAT TACGAGGAGC CAAATATCTG 
CCAGATAAAA ATGGAGTAAC TCCTCTGGAT TTATGTGTAC AGGGTGGATA TGGAGAGACT 
TGTGAAGTAT TAATTCAATA TCACCCGAGG CTTTTTCAGA CTATTATTCA AATGACACAG 
AATGAAGACC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AGCATTTGTC TCAGCAAAGT 
GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCTACAAC AAATGGTCAT 
AAACTGCTTA GCCTCTCTAG CAATTATGAT GCTCAAATGA AGAGCCTTTT AAGGATTGTG 
AGAATGTTTT GTCACGTCTT TCGAATTGGT CCATCCTCCC CCAGTAATGG AATTGATATG 
GGCTACAATG GGAATAAAAC TCCAAGAAGC CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG 
CACTCGTTAG ATGAATGGCT AGTTTTAATA GCCACAGAAT TGATGAAAAA CAAAAGAGAC 
TCAACAGAGA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGCTGCTTCC 
ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTGTCCAC TGGCACAAGG 
GAATCTAAAC CAGATGCTCT TGCAGGGAGA CAGGAAGCCA GTGCAGATTG TCAGGATGTT 
ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT 
TGTCAGATGC CTCCGGGAAT GACTTCACCT CGTTTCATTG AATTTGTCTG CAAACATGAT 
GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGACCA CTTTCACTTT 
CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCTTTT 
AAAGATCGCT GTGAATGGTT CTATGAACAT TTGCATTCAG GACAGCCAGA TTCAGATATG 
GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTGGTTC ACAGAGATTC TATTTTTAGG 
AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT TGTGCAAAGC TAAAGCAAGG GATTGCTGTA 
CGGTTCCATG GAGAAGAAGG CATGGGTCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG 
TCCAATGAGA TAGTCAATCC TGATTATGCA TTGTTTACCC AGTCAGCTGA TGGAACAACT 
TTTCAGCCTA ATAGCAACTC TTATGTAAAT CCTGATCACT TGAACTATTT TCGGTTTGCT 
GGGCAGATCT TGGGATTAGC GTTGAACCAC AGGCAGCTGG TCAATATTTA CTTCACACGA 
TCCTTCTACA AGCACATTCT TGGTATTCCT GTAAATTACC AAGATGTGGC ATCCATTGAT 
CCAGAATATG CGAAAAATTT GCAATGGATT TTAGATAATG ATATAAGTGA TCTGGGTCTA 
GAACTAACTT TTTCTGTTGA GACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA 
CCTGGGGGTG GGAGTATTCT TGTGACACAA AATAATAAAG CGGAGTACGT CCAGCTTGTT 
ACTGAACTTC GAATGACAAG AGCCATTCAG CCTCAGATCA ATGCTTTTTT ACAGGGCTTT 
CATATGTTCA TTCCACCCTC CCTCATACAG CTTTTTGATG AATATGAATT GGAGCTACTG 
CTTTCTGGCA TGCCAGAAAT TGATGTGAGT GATTGGATAA AAAATACAGA ATACACAAGT 
GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTAGA AGACATTACT 
CAAGAGGAGA GAGTTCTTCT CTTACAGTTT GTTACGGGCA GTTCCAGGGT CCCACATGGT 
GGGTTTGCTA ATATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATCGC TGCTGTGCCA 
TATACTCCAA ATCTTTTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA 
TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CACTACATTG TGGCAGCTAT 
GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001350558.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337487.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu40896
Clone ID Related Accession (Same CDS sequence)	NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
Accession Version	NM_001350558.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On Apr 20, 2017 this sequence version replaced XM_017011116.1. Transcript Variant: This variant (10), as well as variants 5 and 9, encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.191812.1, SRR1803612.263449.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337487.1
CDS	699..2924
Translation	MGGQNYSMTLCSMSVMLMLRMPWGRQHCMLPARTVTRRHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001350558.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350558.1

ORF Insert Sequence:

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001350557.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337486.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu40896
Clone ID Related Accession (Same CDS sequence)	NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
Accession Version	NM_001350557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001350557.1. Transcript Variant: This variant (9), as well as variants 5 and 10, encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.1296.1, SRR1660807.101354.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337486.1
CDS	623..2848
Translation	MGGQNYSMTLCSMSVMLMLRMPWGRQHCMLPARTVTRRHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001350557.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350557.2

ORF Insert Sequence:

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001350558.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337487.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu40896
Clone ID Related Accession (Same CDS sequence)	NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
Accession Version	NM_001350558.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001350558.1. Transcript Variant: This variant (10), as well as variants 5 and 9, encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.191812.1, SRR1803612.263449.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337487.1
CDS	699..2924
Translation	MGGQNYSMTLCSMSVMLMLRMPWGRQHCMLPARTVTRRHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001350558.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350558.2

ORF Insert Sequence:

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001321084.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308013.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu40896
Clone ID Related Accession (Same CDS sequence)	NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
Accession Version	NM_001321084.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB094058.1, BC034982.2, AL513472.9 and AL590402.10. On Mar 18, 2016 this sequence version replaced XM_006715530.2. Transcript Variant: This variant (5), as well as variants 9 and 10, encodes isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.167363.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308013.1
CDS	514..2739
Translation	MGGQNYSMTLCSMSVMLMLRMPWGRQHCMLPARTVTRRHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001321084.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321084.1

ORF Insert Sequence:

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001321084.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308013.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu40896
Clone ID Related Accession (Same CDS sequence)	NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
Accession Version	NM_001321084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB094058.1, BC034982.2, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001321084.1. Transcript Variant: This variant (5), as well as variants 9 and 10, encodes isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.167363.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308013.1
CDS	514..2739
Translation	MGGQNYSMTLCSMSVMLMLRMPWGRQHCMLPARTVTRRHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001321084.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321084.2

ORF Insert Sequence:

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001350557.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337486.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu40896
Clone ID Related Accession (Same CDS sequence)	NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
Accession Version	NM_001350557.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On Apr 20, 2017 this sequence version replaced XM_017011120.1. Transcript Variant: This variant (9), as well as variants 5 and 10, encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.1296.1, SRR1660807.101354.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337486.1
CDS	623..2848
Translation	MGGQNYSMTLCSMSVMLMLRMPWGRQHCMLPARTVTRRHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001350557.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350557.1

ORF Insert Sequence:

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001350556.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337485.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu70565
Clone ID Related Accession (Same CDS sequence)	NM_001350556.2 , NM_001350556.1
Accession Version	NM_001350556.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001350556.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.112650.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGA CAGCACTGCA TGTTGCCTGC CAGAACGGTC ACAAGACGAC AGTGCAGTGC 
TTGCTAGACA GTGGTGCTGA TATTAACAGG CCAAATGTAT CAGGAGCAAC TCCATTGTAC 
TTTGCTTGCA GTCATGGTCA GAGAGATACA GCACAGATCC TACTATTACG AGGAGCCAAA 
TATCTGCCAG ATAAAAATGG AGTAACTCCT CTGGATTTAT GTGTACAGGG TGGATATGGA 
GAGACTTGTG AAGTATTAAT TCAATATCAC CCGAGGCTTT TTCAGACTAT TATTCAAATG 
ACACAGAATG AAGACCTCCG AGAAAACATG TTACGGCAAG TTCTGGAGCA TTTGTCTCAG 
CAAAGTGAAA GCCAGTACCT AAAGATTCTA ACAAGCCTTG CTGAAGTTGC TACAACAAAT 
GGTCATAAAC TGCTTAGCCT CTCTAGCAAT TATGATGCTC AAATGAAGAG CCTTTTAAGG 
ATTGTGAGAA TGTTTTGTCA CGTCTTTCGA ATTGGTCCAT CCTCCCCCAG TAATGGAATT 
GATATGGGCT ACAATGGGAA TAAAACTCCA AGAAGCCAGG TGTTCAAGCC TCTGGAATTG 
CTTTGGCACT CGTTAGATGA ATGGCTAGTT TTAATAGCCA CAGAATTGAT GAAAAACAAA 
AGAGACTCAA CAGAGATCAC TTCTATTTTA CTGAAACAAA AAGGCCAAGA TCAAGATGCT 
GCTTCCATTC CTCCATTTGA ACCTCCAGGA CCTGGGAGCT ATGAAAATCT GTCCACTGGC 
ACAAGGGAAT CTAAACCAGA TGCTCTTGCA GGGAGACAGG AAGCCAGTGC AGATTGTCAG 
GATGTTATTT CTATGACAGC TAACCGGCTA AGTGCTGTCA TTCAAGCTTT TTACATGTGC 
TGTTCTTGTC AGATGCCTCC GGGAATGACT TCACCTCGTT TCATTGAATT TGTCTGCAAA 
CATGATGAAG TTTTAAAATG CTTTGTTAAT AGAAATCCCA AAATTATATT TGACCACTTT 
CACTTTCTCC TTGAATGTCC TGAGTTGATG TCAAGATTCA TGCATATCAT AAAAGCACAG 
CCTTTTAAAG ATCGCTGTGA ATGGTTCTAT GAACATTTGC ATTCAGGACA GCCAGATTCA 
GATATGGTGC ACAGGCCAGT GAATGAAAAT GATATCCTGC TGGTTCACAG AGATTCTATT 
TTTAGGAGTA GCTGTGAAGT TGTGTCAAAA GCAAATTGTG CAAAGCTAAA GCAAGGGATT 
GCTGTACGGT TCCATGGAGA AGAAGGCATG GGTCAAGGTG TTGTGCGTGA GTGGTTTGAT 
ATTCTGTCCA ATGAGATAGT CAATCCTGAT TATGCATTGT TTACCCAGTC AGCTGATGGA 
ACAACTTTTC AGCCTAATAG CAACTCTTAT GTAAATCCTG ATCACTTGAA CTATTTTCGG 
TTTGCTGGGC AGATCTTGGG ATTAGCGTTG AACCACAGGC AGCTGGTCAA TATTTACTTC 
ACACGATCCT TCTACAAGCA CATTCTTGGT ATTCCTGTAA ATTACCAAGA TGTGGCATCC 
ATTGATCCAG AATATGCGAA AAATTTGCAA TGGATTTTAG ATAATGATAT AAGTGATCTG 
GGTCTAGAAC TAACTTTTTC TGTTGAGACT GATGTGTTTG GAGCAATGGA AGAGGTGCCT 
TTGAAACCTG GGGGTGGGAG TATTCTTGTG ACACAAAATA ATAAAGCGGA GTACGTCCAG 
CTTGTTACTG AACTTCGAAT GACAAGAGCC ATTCAGCCTC AGATCAATGC TTTTTTACAG 
GGCTTTCATA TGTTCATTCC ACCCTCCCTC ATACAGCTTT TTGATGAATA TGAATTGGAG 
CTACTGCTTT CTGGCATGCC AGAAATTGAT GTGAGTGATT GGATAAAAAA TACAGAATAC 
ACAAGTGGCT ATGAAAGAGA AGATCCAGTT ATTCAGTGGT TCTGGGAAGT TGTAGAAGAC 
ATTACTCAAG AGGAGAGAGT TCTTCTCTTA CAGTTTGTTA CGGGCAGTTC CAGGGTCCCA 
CATGGTGGGT TTGCTAATAT CATGGGTGGA AGTGGATTGC AAAACTTTAC AATCGCTGCT 
GTGCCATATA CTCCAAATCT TTTACCAACT TCAAGCACAT GCATCAACAT GCTCAAGTTA 
CCTGAATACC CAAGTAAAGA AATACTCAAG GACAGACTTC TTGTGGCACT ACATTGTGGC 
AGCTATGGTT ACACAATGGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337485.1
CDS	688..2931
Translation	MGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001350556.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350556.2

ORF Insert Sequence:

ATGGGGCAGA CAGCACTGCA TGTTGCCTGC CAGAACGGTC ACAAGACGAC AGTGCAGTGC 
TTGCTAGACA GTGGTGCTGA TATTAACAGG CCAAATGTAT CAGGAGCAAC TCCATTGTAC 
TTTGCTTGCA GTCATGGTCA GAGAGATACA GCACAGATCC TACTATTACG AGGAGCCAAA 
TATCTGCCAG ATAAAAATGG AGTAACTCCT CTGGATTTAT GTGTACAGGG TGGATATGGA 
GAGACTTGTG AAGTATTAAT TCAATATCAC CCGAGGCTTT TTCAGACTAT TATTCAAATG 
ACACAGAATG AAGACCTCCG AGAAAACATG TTACGGCAAG TTCTGGAGCA TTTGTCTCAG 
CAAAGTGAAA GCCAGTACCT AAAGATTCTA ACAAGCCTTG CTGAAGTTGC TACAACAAAT 
GGTCATAAAC TGCTTAGCCT CTCTAGCAAT TATGATGCTC AAATGAAGAG CCTTTTAAGG 
ATTGTGAGAA TGTTTTGTCA CGTCTTTCGA ATTGGTCCAT CCTCCCCCAG TAATGGAATT 
GATATGGGCT ACAATGGGAA TAAAACTCCA AGAAGCCAGG TGTTCAAGCC TCTGGAATTG 
CTTTGGCACT CGTTAGATGA ATGGCTAGTT TTAATAGCCA CAGAATTGAT GAAAAACAAA 
AGAGACTCAA CAGAGATCAC TTCTATTTTA CTGAAACAAA AAGGCCAAGA TCAAGATGCT 
GCTTCCATTC CTCCATTTGA ACCTCCAGGA CCTGGGAGCT ATGAAAATCT GTCCACTGGC 
ACAAGGGAAT CTAAACCAGA TGCTCTTGCA GGGAGACAGG AAGCCAGTGC AGATTGTCAG 
GATGTTATTT CTATGACAGC TAACCGGCTA AGTGCTGTCA TTCAAGCTTT TTACATGTGC 
TGTTCTTGTC AGATGCCTCC GGGAATGACT TCACCTCGTT TCATTGAATT TGTCTGCAAA 
CATGATGAAG TTTTAAAATG CTTTGTTAAT AGAAATCCCA AAATTATATT TGACCACTTT 
CACTTTCTCC TTGAATGTCC TGAGTTGATG TCAAGATTCA TGCATATCAT AAAAGCACAG 
CCTTTTAAAG ATCGCTGTGA ATGGTTCTAT GAACATTTGC ATTCAGGACA GCCAGATTCA 
GATATGGTGC ACAGGCCAGT GAATGAAAAT GATATCCTGC TGGTTCACAG AGATTCTATT 
TTTAGGAGTA GCTGTGAAGT TGTGTCAAAA GCAAATTGTG CAAAGCTAAA GCAAGGGATT 
GCTGTACGGT TCCATGGAGA AGAAGGCATG GGTCAAGGTG TTGTGCGTGA GTGGTTTGAT 
ATTCTGTCCA ATGAGATAGT CAATCCTGAT TATGCATTGT TTACCCAGTC AGCTGATGGA 
ACAACTTTTC AGCCTAATAG CAACTCTTAT GTAAATCCTG ATCACTTGAA CTATTTTCGG 
TTTGCTGGGC AGATCTTGGG ATTAGCGTTG AACCACAGGC AGCTGGTCAA TATTTACTTC 
ACACGATCCT TCTACAAGCA CATTCTTGGT ATTCCTGTAA ATTACCAAGA TGTGGCATCC 
ATTGATCCAG AATATGCGAA AAATTTGCAA TGGATTTTAG ATAATGATAT AAGTGATCTG 
GGTCTAGAAC TAACTTTTTC TGTTGAGACT GATGTGTTTG GAGCAATGGA AGAGGTGCCT 
TTGAAACCTG GGGGTGGGAG TATTCTTGTG ACACAAAATA ATAAAGCGGA GTACGTCCAG 
CTTGTTACTG AACTTCGAAT GACAAGAGCC ATTCAGCCTC AGATCAATGC TTTTTTACAG 
GGCTTTCATA TGTTCATTCC ACCCTCCCTC ATACAGCTTT TTGATGAATA TGAATTGGAG 
CTACTGCTTT CTGGCATGCC AGAAATTGAT GTGAGTGATT GGATAAAAAA TACAGAATAC 
ACAAGTGGCT ATGAAAGAGA AGATCCAGTT ATTCAGTGGT TCTGGGAAGT TGTAGAAGAC 
ATTACTCAAG AGGAGAGAGT TCTTCTCTTA CAGTTTGTTA CGGGCAGTTC CAGGGTCCCA 
CATGGTGGGT TTGCTAATAT CATGGGTGGA AGTGGATTGC AAAACTTTAC AATCGCTGCT 
GTGCCATATA CTCCAAATCT TTTACCAACT TCAAGCACAT GCATCAACAT GCTCAAGTTA 
CCTGAATACC CAAGTAAAGA AATACTCAAG GACAGACTTC TTGTGGCACT ACATTGTGGC 
AGCTATGGTT ACACAATGGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001350556.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337485.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu70565
Clone ID Related Accession (Same CDS sequence)	NM_001350556.2 , NM_001350556.1
Accession Version	NM_001350556.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On Apr 20, 2017 this sequence version replaced XM_017011115.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.112650.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGA CAGCACTGCA TGTTGCCTGC CAGAACGGTC ACAAGACGAC AGTGCAGTGC 
TTGCTAGACA GTGGTGCTGA TATTAACAGG CCAAATGTAT CAGGAGCAAC TCCATTGTAC 
TTTGCTTGCA GTCATGGTCA GAGAGATACA GCACAGATCC TACTATTACG AGGAGCCAAA 
TATCTGCCAG ATAAAAATGG AGTAACTCCT CTGGATTTAT GTGTACAGGG TGGATATGGA 
GAGACTTGTG AAGTATTAAT TCAATATCAC CCGAGGCTTT TTCAGACTAT TATTCAAATG 
ACACAGAATG AAGACCTCCG AGAAAACATG TTACGGCAAG TTCTGGAGCA TTTGTCTCAG 
CAAAGTGAAA GCCAGTACCT AAAGATTCTA ACAAGCCTTG CTGAAGTTGC TACAACAAAT 
GGTCATAAAC TGCTTAGCCT CTCTAGCAAT TATGATGCTC AAATGAAGAG CCTTTTAAGG 
ATTGTGAGAA TGTTTTGTCA CGTCTTTCGA ATTGGTCCAT CCTCCCCCAG TAATGGAATT 
GATATGGGCT ACAATGGGAA TAAAACTCCA AGAAGCCAGG TGTTCAAGCC TCTGGAATTG 
CTTTGGCACT CGTTAGATGA ATGGCTAGTT TTAATAGCCA CAGAATTGAT GAAAAACAAA 
AGAGACTCAA CAGAGATCAC TTCTATTTTA CTGAAACAAA AAGGCCAAGA TCAAGATGCT 
GCTTCCATTC CTCCATTTGA ACCTCCAGGA CCTGGGAGCT ATGAAAATCT GTCCACTGGC 
ACAAGGGAAT CTAAACCAGA TGCTCTTGCA GGGAGACAGG AAGCCAGTGC AGATTGTCAG 
GATGTTATTT CTATGACAGC TAACCGGCTA AGTGCTGTCA TTCAAGCTTT TTACATGTGC 
TGTTCTTGTC AGATGCCTCC GGGAATGACT TCACCTCGTT TCATTGAATT TGTCTGCAAA 
CATGATGAAG TTTTAAAATG CTTTGTTAAT AGAAATCCCA AAATTATATT TGACCACTTT 
CACTTTCTCC TTGAATGTCC TGAGTTGATG TCAAGATTCA TGCATATCAT AAAAGCACAG 
CCTTTTAAAG ATCGCTGTGA ATGGTTCTAT GAACATTTGC ATTCAGGACA GCCAGATTCA 
GATATGGTGC ACAGGCCAGT GAATGAAAAT GATATCCTGC TGGTTCACAG AGATTCTATT 
TTTAGGAGTA GCTGTGAAGT TGTGTCAAAA GCAAATTGTG CAAAGCTAAA GCAAGGGATT 
GCTGTACGGT TCCATGGAGA AGAAGGCATG GGTCAAGGTG TTGTGCGTGA GTGGTTTGAT 
ATTCTGTCCA ATGAGATAGT CAATCCTGAT TATGCATTGT TTACCCAGTC AGCTGATGGA 
ACAACTTTTC AGCCTAATAG CAACTCTTAT GTAAATCCTG ATCACTTGAA CTATTTTCGG 
TTTGCTGGGC AGATCTTGGG ATTAGCGTTG AACCACAGGC AGCTGGTCAA TATTTACTTC 
ACACGATCCT TCTACAAGCA CATTCTTGGT ATTCCTGTAA ATTACCAAGA TGTGGCATCC 
ATTGATCCAG AATATGCGAA AAATTTGCAA TGGATTTTAG ATAATGATAT AAGTGATCTG 
GGTCTAGAAC TAACTTTTTC TGTTGAGACT GATGTGTTTG GAGCAATGGA AGAGGTGCCT 
TTGAAACCTG GGGGTGGGAG TATTCTTGTG ACACAAAATA ATAAAGCGGA GTACGTCCAG 
CTTGTTACTG AACTTCGAAT GACAAGAGCC ATTCAGCCTC AGATCAATGC TTTTTTACAG 
GGCTTTCATA TGTTCATTCC ACCCTCCCTC ATACAGCTTT TTGATGAATA TGAATTGGAG 
CTACTGCTTT CTGGCATGCC AGAAATTGAT GTGAGTGATT GGATAAAAAA TACAGAATAC 
ACAAGTGGCT ATGAAAGAGA AGATCCAGTT ATTCAGTGGT TCTGGGAAGT TGTAGAAGAC 
ATTACTCAAG AGGAGAGAGT TCTTCTCTTA CAGTTTGTTA CGGGCAGTTC CAGGGTCCCA 
CATGGTGGGT TTGCTAATAT CATGGGTGGA AGTGGATTGC AAAACTTTAC AATCGCTGCT 
GTGCCATATA CTCCAAATCT TTTACCAACT TCAAGCACAT GCATCAACAT GCTCAAGTTA 
CCTGAATACC CAAGTAAAGA AATACTCAAG GACAGACTTC TTGTGGCACT ACATTGTGGC 
AGCTATGGTT ACACAATGGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337485.1
CDS	688..2931
Translation	MGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001350556.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350556.1

ORF Insert Sequence:

ATGGGGCAGA CAGCACTGCA TGTTGCCTGC CAGAACGGTC ACAAGACGAC AGTGCAGTGC 
TTGCTAGACA GTGGTGCTGA TATTAACAGG CCAAATGTAT CAGGAGCAAC TCCATTGTAC 
TTTGCTTGCA GTCATGGTCA GAGAGATACA GCACAGATCC TACTATTACG AGGAGCCAAA 
TATCTGCCAG ATAAAAATGG AGTAACTCCT CTGGATTTAT GTGTACAGGG TGGATATGGA 
GAGACTTGTG AAGTATTAAT TCAATATCAC CCGAGGCTTT TTCAGACTAT TATTCAAATG 
ACACAGAATG AAGACCTCCG AGAAAACATG TTACGGCAAG TTCTGGAGCA TTTGTCTCAG 
CAAAGTGAAA GCCAGTACCT AAAGATTCTA ACAAGCCTTG CTGAAGTTGC TACAACAAAT 
GGTCATAAAC TGCTTAGCCT CTCTAGCAAT TATGATGCTC AAATGAAGAG CCTTTTAAGG 
ATTGTGAGAA TGTTTTGTCA CGTCTTTCGA ATTGGTCCAT CCTCCCCCAG TAATGGAATT 
GATATGGGCT ACAATGGGAA TAAAACTCCA AGAAGCCAGG TGTTCAAGCC TCTGGAATTG 
CTTTGGCACT CGTTAGATGA ATGGCTAGTT TTAATAGCCA CAGAATTGAT GAAAAACAAA 
AGAGACTCAA CAGAGATCAC TTCTATTTTA CTGAAACAAA AAGGCCAAGA TCAAGATGCT 
GCTTCCATTC CTCCATTTGA ACCTCCAGGA CCTGGGAGCT ATGAAAATCT GTCCACTGGC 
ACAAGGGAAT CTAAACCAGA TGCTCTTGCA GGGAGACAGG AAGCCAGTGC AGATTGTCAG 
GATGTTATTT CTATGACAGC TAACCGGCTA AGTGCTGTCA TTCAAGCTTT TTACATGTGC 
TGTTCTTGTC AGATGCCTCC GGGAATGACT TCACCTCGTT TCATTGAATT TGTCTGCAAA 
CATGATGAAG TTTTAAAATG CTTTGTTAAT AGAAATCCCA AAATTATATT TGACCACTTT 
CACTTTCTCC TTGAATGTCC TGAGTTGATG TCAAGATTCA TGCATATCAT AAAAGCACAG 
CCTTTTAAAG ATCGCTGTGA ATGGTTCTAT GAACATTTGC ATTCAGGACA GCCAGATTCA 
GATATGGTGC ACAGGCCAGT GAATGAAAAT GATATCCTGC TGGTTCACAG AGATTCTATT 
TTTAGGAGTA GCTGTGAAGT TGTGTCAAAA GCAAATTGTG CAAAGCTAAA GCAAGGGATT 
GCTGTACGGT TCCATGGAGA AGAAGGCATG GGTCAAGGTG TTGTGCGTGA GTGGTTTGAT 
ATTCTGTCCA ATGAGATAGT CAATCCTGAT TATGCATTGT TTACCCAGTC AGCTGATGGA 
ACAACTTTTC AGCCTAATAG CAACTCTTAT GTAAATCCTG ATCACTTGAA CTATTTTCGG 
TTTGCTGGGC AGATCTTGGG ATTAGCGTTG AACCACAGGC AGCTGGTCAA TATTTACTTC 
ACACGATCCT TCTACAAGCA CATTCTTGGT ATTCCTGTAA ATTACCAAGA TGTGGCATCC 
ATTGATCCAG AATATGCGAA AAATTTGCAA TGGATTTTAG ATAATGATAT AAGTGATCTG 
GGTCTAGAAC TAACTTTTTC TGTTGAGACT GATGTGTTTG GAGCAATGGA AGAGGTGCCT 
TTGAAACCTG GGGGTGGGAG TATTCTTGTG ACACAAAATA ATAAAGCGGA GTACGTCCAG 
CTTGTTACTG AACTTCGAAT GACAAGAGCC ATTCAGCCTC AGATCAATGC TTTTTTACAG 
GGCTTTCATA TGTTCATTCC ACCCTCCCTC ATACAGCTTT TTGATGAATA TGAATTGGAG 
CTACTGCTTT CTGGCATGCC AGAAATTGAT GTGAGTGATT GGATAAAAAA TACAGAATAC 
ACAAGTGGCT ATGAAAGAGA AGATCCAGTT ATTCAGTGGT TCTGGGAAGT TGTAGAAGAC 
ATTACTCAAG AGGAGAGAGT TCTTCTCTTA CAGTTTGTTA CGGGCAGTTC CAGGGTCCCA 
CATGGTGGGT TTGCTAATAT CATGGGTGGA AGTGGATTGC AAAACTTTAC AATCGCTGCT 
GTGCCATATA CTCCAAATCT TTTACCAACT TCAAGCACAT GCATCAACAT GCTCAAGTTA 
CCTGAATACC CAAGTAAAGA AATACTCAAG GACAGACTTC TTGTGGCACT ACATTGTGGC 
AGCTATGGTT ACACAATGGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001350559.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337488.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OHu70566
Clone ID Related Accession (Same CDS sequence)	NM_001350559.1 , NM_001350559.2 , XM_017011122.1
Accession Version	NM_001350559.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On Apr 20, 2017 this sequence version replaced XM_017011121.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.97975.1, SRR1803617.143672.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAACAT TTGTAATAAT GAAGGCCTTA CAGCATCATG GTCAGAGAGA TACAGCACAG 
ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT 
TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG 
CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT 
GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT 
CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC 
CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA 
GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA 
CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA 
CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT 
CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA 
TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT 
TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT 
TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA 
GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA 
TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT 
CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC 
AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT 
GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT 
TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA 
AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG 
CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT 
GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT 
GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA 
TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC 
ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA 
CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337488.1
CDS	645..2792
Translation	MSTFVIMKALQHHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001350559.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350559.1

ORF Insert Sequence:

ATGTCAACAT TTGTAATAAT GAAGGCCTTA CAGCATCATG GTCAGAGAGA TACAGCACAG 
ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT 
TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG 
CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT 
GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT 
CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC 
CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA 
GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA 
CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA 
CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT 
CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA 
TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT 
TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT 
TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA 
GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA 
TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT 
CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC 
AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT 
GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT 
TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA 
AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG 
CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT 
GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT 
GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA 
TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC 
ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA 
CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001350559.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337488.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OHu70566
Clone ID Related Accession (Same CDS sequence)	NM_001350559.1 , NM_001350559.2 , XM_017011122.1
Accession Version	NM_001350559.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001350559.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.97975.1, SRR1803617.143672.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAACAT TTGTAATAAT GAAGGCCTTA CAGCATCATG GTCAGAGAGA TACAGCACAG 
ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT 
TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG 
CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT 
GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT 
CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC 
CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA 
GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA 
CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA 
CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT 
CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA 
TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT 
TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT 
TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA 
GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA 
TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT 
CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC 
AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT 
GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT 
TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA 
AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG 
CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT 
GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT 
GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA 
TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC 
ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA 
CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337488.1
CDS	645..2792
Translation	MSTFVIMKALQHHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001350559.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350559.2

ORF Insert Sequence:

ATGTCAACAT TTGTAATAAT GAAGGCCTTA CAGCATCATG GTCAGAGAGA TACAGCACAG 
ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT 
TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG 
CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT 
GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT 
CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC 
CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA 
GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA 
CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA 
CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT 
CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA 
TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT 
TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT 
TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA 
GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA 
TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT 
CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC 
AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT 
GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT 
TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA 
AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG 
CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT 
GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT 
GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA 
TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC 
ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA 
CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_017011119.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866608.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu40896
Clone ID Related Accession (Same CDS sequence)	NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
Accession Version	XM_017011119.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866608.1
CDS	485..2710
Translation	MGGQNYSMTLCSMSVMLMLRMPWGRQHCMLPARTVTRRHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_017011119.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011119.1

ORF Insert Sequence:

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_017011119.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866608.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu40896
Clone ID Related Accession (Same CDS sequence)	NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
Accession Version	XM_017011119.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866608.1
CDS	788..3013
Translation	MGGQNYSMTLCSMSVMLMLRMPWGRQHCMLPARTVTRRHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_017011119.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011119.2

ORF Insert Sequence:

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_017011118.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866607.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu40896
Clone ID Related Accession (Same CDS sequence)	NM_001350558.1 , NM_001350557.2 , XM_017011119.1 , XM_017011119.2 , NM_001350558.2 , NM_001321084.1 , NM_001321084.2 , XM_017011118.1 , NM_001350557.1
Accession Version	XM_017011118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866607.1
CDS	202..2427
Translation	MGGQNYSMTLCSMSVMLMLRMPWGRQHCMLPARTVTRRHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_017011118.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011118.1

ORF Insert Sequence:

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCATGT CAGTGATGTT GATGTTGAGG 
ATGCCATGGG GCAGACAGCA CTGCATGTTG CCTGCCAGAA CGGTCACAAG ACGTCATGGT 
CAGAGAGATA CAGCACAGAT CCTACTATTA CGAGGAGCCA AATATCTGCC AGATAAAAAT 
GGAGTAACTC CTCTGGATTT ATGTGTACAG GGTGGATATG GAGAGACTTG TGAAGTATTA 
ATTCAATATC ACCCGAGGCT TTTTCAGACT ATTATTCAAA TGACACAGAA TGAAGACCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCTACAACAA ATGGTCATAA ACTGCTTAGC 
CTCTCTAGCA ATTATGATGC TCAAATGAAG AGCCTTTTAA GGATTGTGAG AATGTTTTGT 
CACGTCTTTC GAATTGGTCC ATCCTCCCCC AGTAATGGAA TTGATATGGG CTACAATGGG 
AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT 
GAATGGCTAG TTTTAATAGC CACAGAATTG ATGAAAAACA AAAGAGACTC AACAGAGATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG CTGCTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTGTCCACTG GCACAAGGGA ATCTAAACCA 
GATGCTCTTG CAGGGAGACA GGAAGCCAGT GCAGATTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCGGGAATGA CTTCACCTCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGACCACT TTCACTTTCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCTTTTAA AGATCGCTGT 
GAATGGTTCT ATGAACATTT GCATTCAGGA CAGCCAGATT CAGATATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAATTG TGCAAAGCTA AAGCAAGGGA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC CAATGAGATA 
GTCAATCCTG ATTATGCATT GTTTACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGCAACTCTT ATGTAAATCC TGATCACTTG AACTATTTTC GGTTTGCTGG GCAGATCTTG 
GGATTAGCGT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC CTTCTACAAG 
CACATTCTTG GTATTCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCG 
AAAAATTTGC AATGGATTTT AGATAATGAT ATAAGTGATC TGGGTCTAGA ACTAACTTTT 
TCTGTTGAGA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGG 
AGTATTCTTG TGACACAAAA TAATAAAGCG GAGTACGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAGCC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCCTCCC TCATACAGCT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGTGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTAGAAG ACATTACTCA AGAGGAGAGA 
GTTCTTCTCT TACAGTTTGT TACGGGCAGT TCCAGGGTCC CACATGGTGG GTTTGCTAAT 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATCGCTG CTGTGCCATA TACTCCAAAT 
CTTTTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCA CTACATTGTG GCAGCTATGG TTACACAATG 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_017011122.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866611.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu70566
Clone ID Related Accession (Same CDS sequence)	NM_001350559.1 , NM_001350559.2 , XM_017011122.1
Accession Version	XM_017011122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAT TTGTAATAAT GAAGGCCTTA CAGCATCATG GTCAGAGAGA TACAGCACAG 
ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT 
TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG 
CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT 
GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT 
CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC 
CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA 
GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA 
CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA 
CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT 
CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA 
TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT 
TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT 
TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA 
GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA 
TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT 
CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC 
AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT 
GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT 
TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA 
AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG 
CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT 
GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT 
GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA 
TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC 
ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA 
CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866611.1
CDS	397..2544
Translation	MSTFVIMKALQHHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_017011122.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011122.1

ORF Insert Sequence:

ATGTCAACAT TTGTAATAAT GAAGGCCTTA CAGCATCATG GTCAGAGAGA TACAGCACAG 
ATCCTACTAT TACGAGGAGC CAAATATCTG CCAGATAAAA ATGGAGTAAC TCCTCTGGAT 
TTATGTGTAC AGGGTGGATA TGGAGAGACT TGTGAAGTAT TAATTCAATA TCACCCGAGG 
CTTTTTCAGA CTATTATTCA AATGACACAG AATGAAGACC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCTACAAC AAATGGTCAT AAACTGCTTA GCCTCTCTAG CAATTATGAT 
GCTCAAATGA AGAGCCTTTT AAGGATTGTG AGAATGTTTT GTCACGTCTT TCGAATTGGT 
CCATCCTCCC CCAGTAATGG AATTGATATG GGCTACAATG GGAATAAAAC TCCAAGAAGC 
CAGGTGTTCA AGCCTCTGGA ATTGCTTTGG CACTCGTTAG ATGAATGGCT AGTTTTAATA 
GCCACAGAAT TGATGAAAAA CAAAAGAGAC TCAACAGAGA TCACTTCTAT TTTACTGAAA 
CAAAAAGGCC AAGATCAAGA TGCTGCTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTGTCCAC TGGCACAAGG GAATCTAAAC CAGATGCTCT TGCAGGGAGA 
CAGGAAGCCA GTGCAGATTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCGGGAAT GACTTCACCT 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT 
CCCAAAATTA TATTTGACCA CTTTCACTTT CTCCTTGAAT GTCCTGAGTT GATGTCAAGA 
TTCATGCATA TCATAAAAGC ACAGCCTTTT AAAGATCGCT GTGAATGGTT CTATGAACAT 
TTGCATTCAG GACAGCCAGA TTCAGATATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTGGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAT 
TGTGCAAAGC TAAAGCAAGG GATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGTCAA 
GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCCAATGAGA TAGTCAATCC TGATTATGCA 
TTGTTTACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGCAACTC TTATGTAAAT 
CCTGATCACT TGAACTATTT TCGGTTTGCT GGGCAGATCT TGGGATTAGC GTTGAACCAC 
AGGCAGCTGG TCAATATTTA CTTCACACGA TCCTTCTACA AGCACATTCT TGGTATTCCT 
GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CGAAAAATTT GCAATGGATT 
TTAGATAATG ATATAAGTGA TCTGGGTCTA GAACTAACTT TTTCTGTTGA GACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GGAGTATTCT TGTGACACAA 
AATAATAAAG CGGAGTACGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAG 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCCTC CCTCATACAG 
CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGT 
GATTGGATAA AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTAGA AGACATTACT CAAGAGGAGA GAGTTCTTCT CTTACAGTTT 
GTTACGGGCA GTTCCAGGGT CCCACATGGT GGGTTTGCTA ATATCATGGG TGGAAGTGGA 
TTGCAAAACT TTACAATCGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCAAGC 
ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA 
CTTCTTGTGG CACTACATTG TGGCAGCTAT GGTTACACAA TGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001350555.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337484.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu101567
Clone ID Related Accession (Same CDS sequence)	NM_001350555.1 , NM_001350555.2
Accession Version	NM_001350555.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.14745.1, SRR1803613.279335.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC 
TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA 
CCCCTTCATT TGGCAGCAAG AAATGGTCAT GGTCAGAGAG ATACAGCACA GATCCTACTA 
TTACGAGGAG CCAAATATCT GCCAGATAAA AATGGAGTAA CTCCTCTGGA TTTATGTGTA 
CAGGGTGGAT ATGGAGAGAC TTGTGAAGTA TTAATTCAAT ATCACCCGAG GCTTTTTCAG 
ACTATTATTC AAATGACACA GAATGAAGAC CTCCGAGAAA ACATGTTACG GCAAGTTCTG 
GAGCATTTGT CTCAGCAAAG TGAAAGCCAG TACCTAAAGA TTCTAACAAG CCTTGCTGAA 
GTTGCTACAA CAAATGGTCA TAAACTGCTT AGCCTCTCTA GCAATTATGA TGCTCAAATG 
AAGAGCCTTT TAAGGATTGT GAGAATGTTT TGTCACGTCT TTCGAATTGG TCCATCCTCC 
CCCAGTAATG GAATTGATAT GGGCTACAAT GGGAATAAAA CTCCAAGAAG CCAGGTGTTC 
AAGCCTCTGG AATTGCTTTG GCACTCGTTA GATGAATGGC TAGTTTTAAT AGCCACAGAA 
TTGATGAAAA ACAAAAGAGA CTCAACAGAG ATCACTTCTA TTTTACTGAA ACAAAAAGGC 
CAAGATCAAG ATGCTGCTTC CATTCCTCCA TTTGAACCTC CAGGACCTGG GAGCTATGAA 
AATCTGTCCA CTGGCACAAG GGAATCTAAA CCAGATGCTC TTGCAGGGAG ACAGGAAGCC 
AGTGCAGATT GTCAGGATGT TATTTCTATG ACAGCTAACC GGCTAAGTGC TGTCATTCAA 
GCTTTTTACA TGTGCTGTTC TTGTCAGATG CCTCCGGGAA TGACTTCACC TCGTTTCATT 
GAATTTGTCT GCAAACATGA TGAAGTTTTA AAATGCTTTG TTAATAGAAA TCCCAAAATT 
ATATTTGACC ACTTTCACTT TCTCCTTGAA TGTCCTGAGT TGATGTCAAG ATTCATGCAT 
ATCATAAAAG CACAGCCTTT TAAAGATCGC TGTGAATGGT TCTATGAACA TTTGCATTCA 
GGACAGCCAG ATTCAGATAT GGTGCACAGG CCAGTGAATG AAAATGATAT CCTGCTGGTT 
CACAGAGATT CTATTTTTAG GAGTAGCTGT GAAGTTGTGT CAAAAGCAAA TTGTGCAAAG 
CTAAAGCAAG GGATTGCTGT ACGGTTCCAT GGAGAAGAAG GCATGGGTCA AGGTGTTGTG 
CGTGAGTGGT TTGATATTCT GTCCAATGAG ATAGTCAATC CTGATTATGC ATTGTTTACC 
CAGTCAGCTG ATGGAACAAC TTTTCAGCCT AATAGCAACT CTTATGTAAA TCCTGATCAC 
TTGAACTATT TTCGGTTTGC TGGGCAGATC TTGGGATTAG CGTTGAACCA CAGGCAGCTG 
GTCAATATTT ACTTCACACG ATCCTTCTAC AAGCACATTC TTGGTATTCC TGTAAATTAC 
CAAGATGTGG CATCCATTGA TCCAGAATAT GCGAAAAATT TGCAATGGAT TTTAGATAAT 
GATATAAGTG ATCTGGGTCT AGAACTAACT TTTTCTGTTG AGACTGATGT GTTTGGAGCA 
ATGGAAGAGG TGCCTTTGAA ACCTGGGGGT GGGAGTATTC TTGTGACACA AAATAATAAA 
GCGGAGTACG TCCAGCTTGT TACTGAACTT CGAATGACAA GAGCCATTCA GCCTCAGATC 
AATGCTTTTT TACAGGGCTT TCATATGTTC ATTCCACCCT CCCTCATACA GCTTTTTGAT 
GAATATGAAT TGGAGCTACT GCTTTCTGGC ATGCCAGAAA TTGATGTGAG TGATTGGATA 
AAAAATACAG AATACACAAG TGGCTATGAA AGAGAAGATC CAGTTATTCA GTGGTTCTGG 
GAAGTTGTAG AAGACATTAC TCAAGAGGAG AGAGTTCTTC TCTTACAGTT TGTTACGGGC 
AGTTCCAGGG TCCCACATGG TGGGTTTGCT AATATCATGG GTGGAAGTGG ATTGCAAAAC 
TTTACAATCG CTGCTGTGCC ATATACTCCA AATCTTTTAC CAACTTCAAG CACATGCATC 
AACATGCTCA AGTTACCTGA ATACCCAAGT AAAGAAATAC TCAAGGACAG ACTTCTTGTG 
GCACTACATT GTGGCAGCTA TGGTTACACA ATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337484.1
CDS	278..2716
Translation	MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001350555.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350555.1

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC 
TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA 
CCCCTTCATT TGGCAGCAAG AAATGGTCAT GGTCAGAGAG ATACAGCACA GATCCTACTA 
TTACGAGGAG CCAAATATCT GCCAGATAAA AATGGAGTAA CTCCTCTGGA TTTATGTGTA 
CAGGGTGGAT ATGGAGAGAC TTGTGAAGTA TTAATTCAAT ATCACCCGAG GCTTTTTCAG 
ACTATTATTC AAATGACACA GAATGAAGAC CTCCGAGAAA ACATGTTACG GCAAGTTCTG 
GAGCATTTGT CTCAGCAAAG TGAAAGCCAG TACCTAAAGA TTCTAACAAG CCTTGCTGAA 
GTTGCTACAA CAAATGGTCA TAAACTGCTT AGCCTCTCTA GCAATTATGA TGCTCAAATG 
AAGAGCCTTT TAAGGATTGT GAGAATGTTT TGTCACGTCT TTCGAATTGG TCCATCCTCC 
CCCAGTAATG GAATTGATAT GGGCTACAAT GGGAATAAAA CTCCAAGAAG CCAGGTGTTC 
AAGCCTCTGG AATTGCTTTG GCACTCGTTA GATGAATGGC TAGTTTTAAT AGCCACAGAA 
TTGATGAAAA ACAAAAGAGA CTCAACAGAG ATCACTTCTA TTTTACTGAA ACAAAAAGGC 
CAAGATCAAG ATGCTGCTTC CATTCCTCCA TTTGAACCTC CAGGACCTGG GAGCTATGAA 
AATCTGTCCA CTGGCACAAG GGAATCTAAA CCAGATGCTC TTGCAGGGAG ACAGGAAGCC 
AGTGCAGATT GTCAGGATGT TATTTCTATG ACAGCTAACC GGCTAAGTGC TGTCATTCAA 
GCTTTTTACA TGTGCTGTTC TTGTCAGATG CCTCCGGGAA TGACTTCACC TCGTTTCATT 
GAATTTGTCT GCAAACATGA TGAAGTTTTA AAATGCTTTG TTAATAGAAA TCCCAAAATT 
ATATTTGACC ACTTTCACTT TCTCCTTGAA TGTCCTGAGT TGATGTCAAG ATTCATGCAT 
ATCATAAAAG CACAGCCTTT TAAAGATCGC TGTGAATGGT TCTATGAACA TTTGCATTCA 
GGACAGCCAG ATTCAGATAT GGTGCACAGG CCAGTGAATG AAAATGATAT CCTGCTGGTT 
CACAGAGATT CTATTTTTAG GAGTAGCTGT GAAGTTGTGT CAAAAGCAAA TTGTGCAAAG 
CTAAAGCAAG GGATTGCTGT ACGGTTCCAT GGAGAAGAAG GCATGGGTCA AGGTGTTGTG 
CGTGAGTGGT TTGATATTCT GTCCAATGAG ATAGTCAATC CTGATTATGC ATTGTTTACC 
CAGTCAGCTG ATGGAACAAC TTTTCAGCCT AATAGCAACT CTTATGTAAA TCCTGATCAC 
TTGAACTATT TTCGGTTTGC TGGGCAGATC TTGGGATTAG CGTTGAACCA CAGGCAGCTG 
GTCAATATTT ACTTCACACG ATCCTTCTAC AAGCACATTC TTGGTATTCC TGTAAATTAC 
CAAGATGTGG CATCCATTGA TCCAGAATAT GCGAAAAATT TGCAATGGAT TTTAGATAAT 
GATATAAGTG ATCTGGGTCT AGAACTAACT TTTTCTGTTG AGACTGATGT GTTTGGAGCA 
ATGGAAGAGG TGCCTTTGAA ACCTGGGGGT GGGAGTATTC TTGTGACACA AAATAATAAA 
GCGGAGTACG TCCAGCTTGT TACTGAACTT CGAATGACAA GAGCCATTCA GCCTCAGATC 
AATGCTTTTT TACAGGGCTT TCATATGTTC ATTCCACCCT CCCTCATACA GCTTTTTGAT 
GAATATGAAT TGGAGCTACT GCTTTCTGGC ATGCCAGAAA TTGATGTGAG TGATTGGATA 
AAAAATACAG AATACACAAG TGGCTATGAA AGAGAAGATC CAGTTATTCA GTGGTTCTGG 
GAAGTTGTAG AAGACATTAC TCAAGAGGAG AGAGTTCTTC TCTTACAGTT TGTTACGGGC 
AGTTCCAGGG TCCCACATGG TGGGTTTGCT AATATCATGG GTGGAAGTGG ATTGCAAAAC 
TTTACAATCG CTGCTGTGCC ATATACTCCA AATCTTTTAC CAACTTCAAG CACATGCATC 
AACATGCTCA AGTTACCTGA ATACCCAAGT AAAGAAATAC TCAAGGACAG ACTTCTTGTG 
GCACTACATT GTGGCAGCTA TGGTTACACA ATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001350555.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337484.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu101567
Clone ID Related Accession (Same CDS sequence)	NM_001350555.1 , NM_001350555.2
Accession Version	NM_001350555.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001350555.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.14745.1, SRR1803613.279335.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC 
TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA 
CCCCTTCATT TGGCAGCAAG AAATGGTCAT GGTCAGAGAG ATACAGCACA GATCCTACTA 
TTACGAGGAG CCAAATATCT GCCAGATAAA AATGGAGTAA CTCCTCTGGA TTTATGTGTA 
CAGGGTGGAT ATGGAGAGAC TTGTGAAGTA TTAATTCAAT ATCACCCGAG GCTTTTTCAG 
ACTATTATTC AAATGACACA GAATGAAGAC CTCCGAGAAA ACATGTTACG GCAAGTTCTG 
GAGCATTTGT CTCAGCAAAG TGAAAGCCAG TACCTAAAGA TTCTAACAAG CCTTGCTGAA 
GTTGCTACAA CAAATGGTCA TAAACTGCTT AGCCTCTCTA GCAATTATGA TGCTCAAATG 
AAGAGCCTTT TAAGGATTGT GAGAATGTTT TGTCACGTCT TTCGAATTGG TCCATCCTCC 
CCCAGTAATG GAATTGATAT GGGCTACAAT GGGAATAAAA CTCCAAGAAG CCAGGTGTTC 
AAGCCTCTGG AATTGCTTTG GCACTCGTTA GATGAATGGC TAGTTTTAAT AGCCACAGAA 
TTGATGAAAA ACAAAAGAGA CTCAACAGAG ATCACTTCTA TTTTACTGAA ACAAAAAGGC 
CAAGATCAAG ATGCTGCTTC CATTCCTCCA TTTGAACCTC CAGGACCTGG GAGCTATGAA 
AATCTGTCCA CTGGCACAAG GGAATCTAAA CCAGATGCTC TTGCAGGGAG ACAGGAAGCC 
AGTGCAGATT GTCAGGATGT TATTTCTATG ACAGCTAACC GGCTAAGTGC TGTCATTCAA 
GCTTTTTACA TGTGCTGTTC TTGTCAGATG CCTCCGGGAA TGACTTCACC TCGTTTCATT 
GAATTTGTCT GCAAACATGA TGAAGTTTTA AAATGCTTTG TTAATAGAAA TCCCAAAATT 
ATATTTGACC ACTTTCACTT TCTCCTTGAA TGTCCTGAGT TGATGTCAAG ATTCATGCAT 
ATCATAAAAG CACAGCCTTT TAAAGATCGC TGTGAATGGT TCTATGAACA TTTGCATTCA 
GGACAGCCAG ATTCAGATAT GGTGCACAGG CCAGTGAATG AAAATGATAT CCTGCTGGTT 
CACAGAGATT CTATTTTTAG GAGTAGCTGT GAAGTTGTGT CAAAAGCAAA TTGTGCAAAG 
CTAAAGCAAG GGATTGCTGT ACGGTTCCAT GGAGAAGAAG GCATGGGTCA AGGTGTTGTG 
CGTGAGTGGT TTGATATTCT GTCCAATGAG ATAGTCAATC CTGATTATGC ATTGTTTACC 
CAGTCAGCTG ATGGAACAAC TTTTCAGCCT AATAGCAACT CTTATGTAAA TCCTGATCAC 
TTGAACTATT TTCGGTTTGC TGGGCAGATC TTGGGATTAG CGTTGAACCA CAGGCAGCTG 
GTCAATATTT ACTTCACACG ATCCTTCTAC AAGCACATTC TTGGTATTCC TGTAAATTAC 
CAAGATGTGG CATCCATTGA TCCAGAATAT GCGAAAAATT TGCAATGGAT TTTAGATAAT 
GATATAAGTG ATCTGGGTCT AGAACTAACT TTTTCTGTTG AGACTGATGT GTTTGGAGCA 
ATGGAAGAGG TGCCTTTGAA ACCTGGGGGT GGGAGTATTC TTGTGACACA AAATAATAAA 
GCGGAGTACG TCCAGCTTGT TACTGAACTT CGAATGACAA GAGCCATTCA GCCTCAGATC 
AATGCTTTTT TACAGGGCTT TCATATGTTC ATTCCACCCT CCCTCATACA GCTTTTTGAT 
GAATATGAAT TGGAGCTACT GCTTTCTGGC ATGCCAGAAA TTGATGTGAG TGATTGGATA 
AAAAATACAG AATACACAAG TGGCTATGAA AGAGAAGATC CAGTTATTCA GTGGTTCTGG 
GAAGTTGTAG AAGACATTAC TCAAGAGGAG AGAGTTCTTC TCTTACAGTT TGTTACGGGC 
AGTTCCAGGG TCCCACATGG TGGGTTTGCT AATATCATGG GTGGAAGTGG ATTGCAAAAC 
TTTACAATCG CTGCTGTGCC ATATACTCCA AATCTTTTAC CAACTTCAAG CACATGCATC 
AACATGCTCA AGTTACCTGA ATACCCAAGT AAAGAAATAC TCAAGGACAG ACTTCTTGTG 
GCACTACATT GTGGCAGCTA TGGTTACACA ATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337484.1
CDS	278..2716
Translation	MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001350555.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350555.2

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA ACTCAACCGC CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGTTGC CCGAGGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAACTACTA TCAAATTCAA AATTTGATGT CAATTATGCA 
TTCGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC GGTGGAATGC 
TTGGTTTTGC TGTTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGCTGTACA 
CCCCTTCATT TGGCAGCAAG AAATGGTCAT GGTCAGAGAG ATACAGCACA GATCCTACTA 
TTACGAGGAG CCAAATATCT GCCAGATAAA AATGGAGTAA CTCCTCTGGA TTTATGTGTA 
CAGGGTGGAT ATGGAGAGAC TTGTGAAGTA TTAATTCAAT ATCACCCGAG GCTTTTTCAG 
ACTATTATTC AAATGACACA GAATGAAGAC CTCCGAGAAA ACATGTTACG GCAAGTTCTG 
GAGCATTTGT CTCAGCAAAG TGAAAGCCAG TACCTAAAGA TTCTAACAAG CCTTGCTGAA 
GTTGCTACAA CAAATGGTCA TAAACTGCTT AGCCTCTCTA GCAATTATGA TGCTCAAATG 
AAGAGCCTTT TAAGGATTGT GAGAATGTTT TGTCACGTCT TTCGAATTGG TCCATCCTCC 
CCCAGTAATG GAATTGATAT GGGCTACAAT GGGAATAAAA CTCCAAGAAG CCAGGTGTTC 
AAGCCTCTGG AATTGCTTTG GCACTCGTTA GATGAATGGC TAGTTTTAAT AGCCACAGAA 
TTGATGAAAA ACAAAAGAGA CTCAACAGAG ATCACTTCTA TTTTACTGAA ACAAAAAGGC 
CAAGATCAAG ATGCTGCTTC CATTCCTCCA TTTGAACCTC CAGGACCTGG GAGCTATGAA 
AATCTGTCCA CTGGCACAAG GGAATCTAAA CCAGATGCTC TTGCAGGGAG ACAGGAAGCC 
AGTGCAGATT GTCAGGATGT TATTTCTATG ACAGCTAACC GGCTAAGTGC TGTCATTCAA 
GCTTTTTACA TGTGCTGTTC TTGTCAGATG CCTCCGGGAA TGACTTCACC TCGTTTCATT 
GAATTTGTCT GCAAACATGA TGAAGTTTTA AAATGCTTTG TTAATAGAAA TCCCAAAATT 
ATATTTGACC ACTTTCACTT TCTCCTTGAA TGTCCTGAGT TGATGTCAAG ATTCATGCAT 
ATCATAAAAG CACAGCCTTT TAAAGATCGC TGTGAATGGT TCTATGAACA TTTGCATTCA 
GGACAGCCAG ATTCAGATAT GGTGCACAGG CCAGTGAATG AAAATGATAT CCTGCTGGTT 
CACAGAGATT CTATTTTTAG GAGTAGCTGT GAAGTTGTGT CAAAAGCAAA TTGTGCAAAG 
CTAAAGCAAG GGATTGCTGT ACGGTTCCAT GGAGAAGAAG GCATGGGTCA AGGTGTTGTG 
CGTGAGTGGT TTGATATTCT GTCCAATGAG ATAGTCAATC CTGATTATGC ATTGTTTACC 
CAGTCAGCTG ATGGAACAAC TTTTCAGCCT AATAGCAACT CTTATGTAAA TCCTGATCAC 
TTGAACTATT TTCGGTTTGC TGGGCAGATC TTGGGATTAG CGTTGAACCA CAGGCAGCTG 
GTCAATATTT ACTTCACACG ATCCTTCTAC AAGCACATTC TTGGTATTCC TGTAAATTAC 
CAAGATGTGG CATCCATTGA TCCAGAATAT GCGAAAAATT TGCAATGGAT TTTAGATAAT 
GATATAAGTG ATCTGGGTCT AGAACTAACT TTTTCTGTTG AGACTGATGT GTTTGGAGCA 
ATGGAAGAGG TGCCTTTGAA ACCTGGGGGT GGGAGTATTC TTGTGACACA AAATAATAAA 
GCGGAGTACG TCCAGCTTGT TACTGAACTT CGAATGACAA GAGCCATTCA GCCTCAGATC 
AATGCTTTTT TACAGGGCTT TCATATGTTC ATTCCACCCT CCCTCATACA GCTTTTTGAT 
GAATATGAAT TGGAGCTACT GCTTTCTGGC ATGCCAGAAA TTGATGTGAG TGATTGGATA 
AAAAATACAG AATACACAAG TGGCTATGAA AGAGAAGATC CAGTTATTCA GTGGTTCTGG 
GAAGTTGTAG AAGACATTAC TCAAGAGGAG AGAGTTCTTC TCTTACAGTT TGTTACGGGC 
AGTTCCAGGG TCCCACATGG TGGGTTTGCT AATATCATGG GTGGAAGTGG ATTGCAAAAC 
TTTACAATCG CTGCTGTGCC ATATACTCCA AATCTTTTAC CAACTTCAAG CACATGCATC 
AACATGCTCA AGTTACCTGA ATACCCAAGT AAAGAAATAC TCAAGGACAG ACTTCTTGTG 
GCACTACATT GTGGCAGCTA TGGTTACACA ATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001350554.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337483.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu96713
Clone ID Related Accession (Same CDS sequence)	NM_001350554.1 , XM_017011114.2 , XM_017011114.1 , NM_001350554.2
Accession Version	NM_001350554.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On Apr 20, 2017 this sequence version replaced XM_017011113.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.96954.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG 
AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA 
ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA 
ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA 
CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG 
GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT 
ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG 
CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT 
GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG 
AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC 
AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG 
CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG 
ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA 
GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT 
CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT 
GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT 
TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA 
TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA 
TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC 
ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA 
CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC 
AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA 
AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT 
GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG 
TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG 
AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC 
AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA 
GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT 
ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG 
GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG 
GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT 
GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA 
TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA 
AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA 
GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT 
TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT 
ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC 
ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA 
CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337483.1
CDS	438..2933
Translation	MPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTATVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001350554.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350554.1

ORF Insert Sequence:

ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG 
AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA 
ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA 
ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA 
CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG 
GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT 
ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG 
CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT 
GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG 
AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC 
AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG 
CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG 
ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA 
GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT 
CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT 
GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT 
TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA 
TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA 
TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC 
ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA 
CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC 
AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA 
AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT 
GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG 
TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG 
AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC 
AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA 
GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT 
ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG 
GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG 
GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT 
GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA 
TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA 
AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA 
GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT 
TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT 
ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC 
ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA 
CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( NM_001350554.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337483.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu96713
Clone ID Related Accession (Same CDS sequence)	NM_001350554.1 , XM_017011114.2 , XM_017011114.1 , NM_001350554.2
Accession Version	NM_001350554.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357315.14, AL513472.9 and AL590402.10. On May 31, 2019 this sequence version replaced NM_001350554.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.96954.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG 
AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA 
ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA 
ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA 
CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG 
GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT 
ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG 
CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT 
GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG 
AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC 
AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG 
CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG 
ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA 
GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT 
CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT 
GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT 
TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA 
TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA 
TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC 
ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA 
CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC 
AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA 
AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT 
GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG 
TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG 
AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC 
AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA 
GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT 
ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG 
GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG 
GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT 
GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA 
TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA 
AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA 
GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT 
TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT 
ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC 
ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA 
CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337483.1
CDS	438..2933
Translation	MPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTATVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	NM_001350554.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350554.2

ORF Insert Sequence:

ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG 
AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA 
ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA 
ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA 
CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG 
GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT 
ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG 
CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT 
GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG 
AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC 
AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG 
CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG 
ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA 
GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT 
CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT 
GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT 
TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA 
TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA 
TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC 
ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA 
CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC 
AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA 
AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT 
GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG 
TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG 
AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC 
AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA 
GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT 
ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG 
GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG 
GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT 
GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA 
TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA 
AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA 
GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT 
TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT 
ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC 
ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA 
CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_017011114.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866603.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu96713
Clone ID Related Accession (Same CDS sequence)	NM_001350554.1 , XM_017011114.2 , XM_017011114.1 , NM_001350554.2
Accession Version	XM_017011114.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011114.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG 
AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA 
ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA 
ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA 
CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG 
GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT 
ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG 
CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT 
GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG 
AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC 
AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG 
CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG 
ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA 
GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT 
CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT 
GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT 
TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA 
TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA 
TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC 
ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA 
CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC 
AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA 
AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT 
GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG 
TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG 
AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC 
AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA 
GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT 
ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG 
GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG 
GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT 
GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA 
TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA 
AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA 
GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT 
TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT 
ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC 
ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA 
CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866603.1
CDS	529..3024
Translation	MPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTATVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_017011114.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011114.2

ORF Insert Sequence:

ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG 
AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA 
ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA 
ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA 
CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG 
GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT 
ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG 
CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT 
GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG 
AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC 
AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG 
CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG 
ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA 
GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT 
CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT 
GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT 
TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA 
TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA 
TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC 
ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA 
CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC 
AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA 
AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT 
GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG 
TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG 
AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC 
AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA 
GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT 
ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG 
GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG 
GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT 
GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA 
TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA 
AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA 
GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT 
TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT 
ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC 
ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA 
CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_017011114.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866603.1 Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu96713
Clone ID Related Accession (Same CDS sequence)	NM_001350554.1 , XM_017011114.2 , XM_017011114.1 , NM_001350554.2
Accession Version	XM_017011114.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HACE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG 
AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA 
ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA 
ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA 
CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG 
GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT 
ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG 
CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT 
GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG 
AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC 
AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG 
CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG 
ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA 
GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT 
CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT 
GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT 
TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA 
TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA 
TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC 
ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA 
CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC 
AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA 
AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT 
GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG 
TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG 
AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC 
AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA 
GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT 
ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG 
GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG 
GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT 
GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA 
TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA 
AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA 
GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT 
TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT 
ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC 
ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA 
CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866603.1
CDS	198..2693
Translation	MPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTATVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_017011114.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011114.1

ORF Insert Sequence:

ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AACTACTATC AAATTCAAAA 
TTTGATGTCA ATTATGCATT CGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCGG TGGAATGCTT GGTTTTGCTG TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCAG GCTGTACACC CCTTCATTTG GCAGCAAGAA ATGGGCAGAA GAAATGTATG 
AGTAAATTAT TAGAATATAG CGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA 
ACAGTGCAGT GCTTGCTAGA CAGTGGTGCT GATATTAACA GGCCAAATGT ATCAGGAGCA 
ACTCCATTGT ACTTTGCTTG CAGTCATGGT CAGAGAGATA CAGCACAGAT CCTACTATTA 
CGAGGAGCCA AATATCTGCC AGATAAAAAT GGAGTAACTC CTCTGGATTT ATGTGTACAG 
GGTGGATATG GAGAGACTTG TGAAGTATTA ATTCAATATC ACCCGAGGCT TTTTCAGACT 
ATTATTCAAA TGACACAGAA TGAAGACCTC CGAGAAAACA TGTTACGGCA AGTTCTGGAG 
CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATTC TAACAAGCCT TGCTGAAGTT 
GCTACAACAA ATGGTCATAA ACTGCTTAGC CTCTCTAGCA ATTATGATGC TCAAATGAAG 
AGCCTTTTAA GGATTGTGAG AATGTTTTGT CACGTCTTTC GAATTGGTCC ATCCTCCCCC 
AGTAATGGAA TTGATATGGG CTACAATGGG AATAAAACTC CAAGAAGCCA GGTGTTCAAG 
CCTCTGGAAT TGCTTTGGCA CTCGTTAGAT GAATGGCTAG TTTTAATAGC CACAGAATTG 
ATGAAAAACA AAAGAGACTC AACAGAGATC ACTTCTATTT TACTGAAACA AAAAGGCCAA 
GATCAAGATG CTGCTTCCAT TCCTCCATTT GAACCTCCAG GACCTGGGAG CTATGAAAAT 
CTGTCCACTG GCACAAGGGA ATCTAAACCA GATGCTCTTG CAGGGAGACA GGAAGCCAGT 
GCAGATTGTC AGGATGTTAT TTCTATGACA GCTAACCGGC TAAGTGCTGT CATTCAAGCT 
TTTTACATGT GCTGTTCTTG TCAGATGCCT CCGGGAATGA CTTCACCTCG TTTCATTGAA 
TTTGTCTGCA AACATGATGA AGTTTTAAAA TGCTTTGTTA ATAGAAATCC CAAAATTATA 
TTTGACCACT TTCACTTTCT CCTTGAATGT CCTGAGTTGA TGTCAAGATT CATGCATATC 
ATAAAAGCAC AGCCTTTTAA AGATCGCTGT GAATGGTTCT ATGAACATTT GCATTCAGGA 
CAGCCAGATT CAGATATGGT GCACAGGCCA GTGAATGAAA ATGATATCCT GCTGGTTCAC 
AGAGATTCTA TTTTTAGGAG TAGCTGTGAA GTTGTGTCAA AAGCAAATTG TGCAAAGCTA 
AAGCAAGGGA TTGCTGTACG GTTCCATGGA GAAGAAGGCA TGGGTCAAGG TGTTGTGCGT 
GAGTGGTTTG ATATTCTGTC CAATGAGATA GTCAATCCTG ATTATGCATT GTTTACCCAG 
TCAGCTGATG GAACAACTTT TCAGCCTAAT AGCAACTCTT ATGTAAATCC TGATCACTTG 
AACTATTTTC GGTTTGCTGG GCAGATCTTG GGATTAGCGT TGAACCACAG GCAGCTGGTC 
AATATTTACT TCACACGATC CTTCTACAAG CACATTCTTG GTATTCCTGT AAATTACCAA 
GATGTGGCAT CCATTGATCC AGAATATGCG AAAAATTTGC AATGGATTTT AGATAATGAT 
ATAAGTGATC TGGGTCTAGA ACTAACTTTT TCTGTTGAGA CTGATGTGTT TGGAGCAATG 
GAAGAGGTGC CTTTGAAACC TGGGGGTGGG AGTATTCTTG TGACACAAAA TAATAAAGCG 
GAGTACGTCC AGCTTGTTAC TGAACTTCGA ATGACAAGAG CCATTCAGCC TCAGATCAAT 
GCTTTTTTAC AGGGCTTTCA TATGTTCATT CCACCCTCCC TCATACAGCT TTTTGATGAA 
TATGAATTGG AGCTACTGCT TTCTGGCATG CCAGAAATTG ATGTGAGTGA TTGGATAAAA 
AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA 
GTTGTAGAAG ACATTACTCA AGAGGAGAGA GTTCTTCTCT TACAGTTTGT TACGGGCAGT 
TCCAGGGTCC CACATGGTGG GTTTGCTAAT ATCATGGGTG GAAGTGGATT GCAAAACTTT 
ACAATCGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCAAGCAC ATGCATCAAC 
ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT TCTTGTGGCA 
CTACATTGTG GCAGCTATGG TTACACAATG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HACE1
Entrez Gene ID	57531
Full Name	HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1
Synonyms	SPPRS
General protein information	Preferred Names HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase HACE1 HECT-type E3 ubiquitin transferase HACE1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q16.3
Summary	This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor. [provided by RefSeq, Mar 2016].
Disorder MIM:	610876
Disorder Html:	Spastic paraplegia and psychomotor retardation with or without seizures, 616756 (3)

Homo sapiens (human)	HACE1	NP_065822.2
Canis lupus familiaris (dog)	HACE1	XP_854232.1
Pan troglodytes (chimpanzee)	HACE1	XP_518654.2
Rattus norvegicus (Norway rat)	Hace1	NP_001102009.2
Gallus gallus (chicken)	HACE1	NP_001265084.1
Xenopus tropicalis (tropical clawed frog)	hace1	NP_001039269.1
Bos taurus (cattle)	HACE1	NP_001179574.2
Mus musculus (house mouse)	Hace1	NP_766061.2
Danio rerio (zebrafish)	hace1	XP_701235.5

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HACE1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: