TRIM54 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRIM54
Entrez Gene ID	57159
Full Name	tripartite motif containing 54
Synonyms	MURF,MURF-3,RNF30,muRF3
General protein information	Preferred Names tripartite motif containing 54 Names tripartite motif-containing protein 54 muscle-specific RING finger protein 3 ring finger protein 30
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p23.3
Summary	The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008].
Disorder MIM:	606474

mRNA and Protein(s)

mRNA	Protein	Name
NM_187841.2	NP_912730.2	tripartite motif-containing protein 54 isoform 2
NM_032546.4	NP_115935.3	tripartite motif-containing protein 54 isoform 1
XM_024453012.1	XP_024308780.1	tripartite motif-containing protein 54 isoform X3
XM_024453011.1	XP_024308779.1	tripartite motif-containing protein 54 isoform X2
NM_032546.3	NP_115935.3	tripartite motif-containing protein 54 isoform 1
NM_187841.3	NP_912730.2	tripartite motif-containing protein 54 isoform 2
XM_024453010.1	XP_024308778.1	tripartite motif-containing protein 54 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Trim54	NP_001013235.1
Bos taurus (cattle)	TRIM54	NP_001017951.1
Pan troglodytes (chimpanzee)	TRIM54	XP_001155913.1
Canis lupus familiaris (dog)	TRIM54	XP_005630313.1
Mus musculus (house mouse)	Trim54	NP_067422.1
Danio rerio (zebrafish)	trim54	NP_001038490.2
Homo sapiens (human)	TRIM54	NP_912730.2
	TRIM54	XP_001092313.1

Gene Ontology
Bibliography

Related articles in PubMed

New cardiac and skeletal protein aggregate myopathy associated with combined MuRF1 and MuRF3 mutations.
Oliv? M, Abdul-Hussein S, Oldfors A, Gonz?lez-Costello J, van der Ven PF, F?rst DO, Gonz?lez L, Moreno D, Torrej?n-Escribano B, Ali? J, Pou A, Ferrer I, Tajsharghi H
Human molecular genetics24(13)3638-50(2015 Jul)

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kov?cs IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barab?si AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain.
Centner T, Yano J, Kimura E, McElhinny AS, Pelin K, Witt CC, Bang ML, Trombitas K, Granzier H, Gregorio CC, Sorimachi H, Labeit S
Journal of molecular biology306(4)717-26(2001 Mar)

Regulation of microtubule dynamics and myogenic differentiation by MURF, a striated muscle RING-finger protein.
Spencer JA, Eliazer S, Ilaria RL Jr, Richardson JA, Olson EN
The Journal of cell biology150(4)771-84(2000 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel protein aggregate myopathies and cardiomyopathy resulting from combined homozygous MuRF1 null mutation and heterozygous MuRF3 missense mutation.
Title: New cardiac and skeletal protein aggregate myopathy associated with combined MuRF1 and MuRF3 mutations.

Functional analysis of the mouse counterpart.
Title: Regulation of microtubule dynamics and myogenic differentiation by MURF, a striated muscle RING-finger protein.

The following TRIM54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIM54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05043	NM_032546.4 Latest version!	Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$169.50-$237.30 $339.00
OHu55662	NM_187841.3 Latest version!	Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$149.50-$209.30 $299.00
OHu55662	XM_024453010.1 Latest version!	Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$149.50-$209.30 $299.00
OHu104924	XM_024453011.1 Latest version!	Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu104925	XM_024453012.1 Latest version!	Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu05043	NM_032546.3	Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$169.50-$237.30 $339.00
OHu55662	NM_187841.2	Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$149.50-$209.30 $299.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRIM54 ( NM_032546.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_115935.3 Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05043
Clone ID Related Accession (Same CDS sequence)	NM_032546.4 , NM_032546.3
Accession Version	NM_032546.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 54 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA897004.1, AJ291714.1 and BC141807.1. On Jun 2, 2019 this sequence version replaced NM_032546.3. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: DRR138527.57412.1, AJ291714.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTTCA CAGTGGGTTT CAAGCCGCTG CTAGGGGATG CACACAGCAT GGACAACCTG 
GAGAAGCAGC TCATCTGCCC CATCTGCCTG GAGATGTTCT CCAAACCAGT GGTGATCCTG 
CCCTGCCAAC ACAACCTGTG CCGCAAATGT GCCAACGACG TCTTCCAGGC CTCGAATCCT 
CTATGGCAGT CCCGGGGCTC CACCACTGTG TCTTCAGGAG GCCGTTTCCG CTGCCCATCG 
TGCAGGCATG AGGTTGTCCT GGACAGACAC GGTGTCTACG GCCTGCAGCG AAACCTGCTA 
GTGGAGAACA TTATCGACAT TTACAAGCAG GAGTCATCCA GGCCGCTGCA CTCCAAGGCT 
GAGCAGCACC TCATGTGCGA GGAGCATGAA GAAGAGAAGA TCAATATTTA CTGCCTGAGC 
TGTGAGGTGC CCACCTGCTC TCTCTGCAAG GTCTTCGGTG CCCACAAGGA CTGTGAGGTG 
GCCCCACTGC CCACCATTTA CAAACGCCAG AAGAAACAGG ATCTCACTCT GTTGCCCAGG 
CTGGAGTGCA GTGGCACAAA CACAACTTAC TGCAGCCTTG ATCTCCCGAG CTCAAGTGAT 
CCTCCCATCT TAGCCTCGCA GAACACTAAG ATTATAGATA GTGAGCTCAG CGATGGCATC 
GCGATGCTGG TGGCAGGCAA TGACCGCGTG CAAGCAGTGA TCACACAGAT GGAGGAGGTG 
TGCCAGACTA TCGAGGACAA TAGCCGGAGG CAGAAGCAGT TGTTAAACCA GAGGTTTGAG 
AGCCTGTGCG CAGTGCTGGA GGAGCGCAAG GGTGAGCTGC TGCAGGCGCT GGCCCGGGAG 
CAAGAGGAGA AGCTGCAGCG CGTCCGCGGC CTCATCCGTC AGTATGGCGA CCACCTGGAG 
GCCTCCTCTA AGCTGGTGGA GTCTGCCATC CAGTCCATGG AAGAGCCACA AATGGCGCTG 
TATCTCCAGC AGGCCAAGGA GCTGATCAAT AAGGTCGGGG CCATGTCGAA GGTGGAGCTG 
GCAGGGCGGC CGGAGCCAGG CTATGAGAGC ATGGAGCAAT TCACCGTAAG GGTGGAGCAC 
GTGGCCGAAA TGCTGCGGAC CATCGACTTC CAGCCAGGCG CTTCCGGGGA GGAAGAGGAG 
GTGGCCCCAG ACGGAGAGGA GGGCAGCGCG GGGCCGGAGG AAGAGCGGCC GGATGGGCCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115935.3
CDS	304..1506
Translation	MNFTVGFKPLLGDAHSMDNLEKQLICPICLEMFSKPVVILPCQHNLCRKCANDVFQASNPLWQSRGSTTVSSGGRFRCPSCRHEVVLDRHGVYGLQRNLLVENIIDIYKQESSRPLHSKAEQHLMCEEHEEEKINIYCLSCEVPTCSLCKVFGAHKDCEVAPLPTIYKRQKKQDLTLLPRLECSGTNTTYCSLDLPSSSDPPILASQNTKIIDSELSDGIAMLVAGNDRVQAVITQMEEVCQTIEDNSRRQKQLLNQRFESLCAVLEERKGELLQALAREQEEKLQRVRGLIRQYGDHLEASSKLVESAIQSMEEPQMALYLQQAKELINKVGAMSKVELAGRPEPGYESMEQFTVRVEHVAEMLRTIDFQPGASGEEEEVAPDGEEGSAGPEEERPDGP

Target ORF information:

RefSeq Version	NM_032546.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032546.4

ORF Insert Sequence:

ATGAACTTCA CAGTGGGTTT CAAGCCGCTG CTAGGGGATG CACACAGCAT GGACAACCTG 
GAGAAGCAGC TCATCTGCCC CATCTGCCTG GAGATGTTCT CCAAACCAGT GGTGATCCTG 
CCCTGCCAAC ACAACCTGTG CCGCAAATGT GCCAACGACG TCTTCCAGGC CTCGAATCCT 
CTATGGCAGT CCCGGGGCTC CACCACTGTG TCTTCAGGAG GCCGTTTCCG CTGCCCATCG 
TGCAGGCATG AGGTTGTCCT GGACAGACAC GGTGTCTACG GCCTGCAGCG AAACCTGCTA 
GTGGAGAACA TTATCGACAT TTACAAGCAG GAGTCATCCA GGCCGCTGCA CTCCAAGGCT 
GAGCAGCACC TCATGTGCGA GGAGCATGAA GAAGAGAAGA TCAATATTTA CTGCCTGAGC 
TGTGAGGTGC CCACCTGCTC TCTCTGCAAG GTCTTCGGTG CCCACAAGGA CTGTGAGGTG 
GCCCCACTGC CCACCATTTA CAAACGCCAG AAGAAACAGG ATCTCACTCT GTTGCCCAGG 
CTGGAGTGCA GTGGCACAAA CACAACTTAC TGCAGCCTTG ATCTCCCGAG CTCAAGTGAT 
CCTCCCATCT TAGCCTCGCA GAACACTAAG ATTATAGATA GTGAGCTCAG CGATGGCATC 
GCGATGCTGG TGGCAGGCAA TGACCGCGTG CAAGCAGTGA TCACACAGAT GGAGGAGGTG 
TGCCAGACTA TCGAGGACAA TAGCCGGAGG CAGAAGCAGT TGTTAAACCA GAGGTTTGAG 
AGCCTGTGCG CAGTGCTGGA GGAGCGCAAG GGTGAGCTGC TGCAGGCGCT GGCCCGGGAG 
CAAGAGGAGA AGCTGCAGCG CGTCCGCGGC CTCATCCGTC AGTATGGCGA CCACCTGGAG 
GCCTCCTCTA AGCTGGTGGA GTCTGCCATC CAGTCCATGG AAGAGCCACA AATGGCGCTG 
TATCTCCAGC AGGCCAAGGA GCTGATCAAT AAGGTCGGGG CCATGTCGAA GGTGGAGCTG 
GCAGGGCGGC CGGAGCCAGG CTATGAGAGC ATGGAGCAAT TCACCGTAAG GGTGGAGCAC 
GTGGCCGAAA TGCTGCGGAC CATCGACTTC CAGCCAGGCG CTTCCGGGGA GGAAGAGGAG 
GTGGCCCCAG ACGGAGAGGA GGGCAGCGCG GGGCCGGAGG AAGAGCGGCC GGATGGGCCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM54 ( NM_032546.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_115935.3 Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05043
Clone ID Related Accession (Same CDS sequence)	NM_032546.4 , NM_032546.3
Accession Version	NM_032546.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 54 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA897004.1, AJ291714.1, BC141807.1 and CA423735.1. On Jul 30, 2010 this sequence version replaced NM_032546.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AJ291714.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTTCA CAGTGGGTTT CAAGCCGCTG CTAGGGGATG CACACAGCAT GGACAACCTG 
GAGAAGCAGC TCATCTGCCC CATCTGCCTG GAGATGTTCT CCAAACCAGT GGTGATCCTG 
CCCTGCCAAC ACAACCTGTG CCGCAAATGT GCCAACGACG TCTTCCAGGC CTCGAATCCT 
CTATGGCAGT CCCGGGGCTC CACCACTGTG TCTTCAGGAG GCCGTTTCCG CTGCCCATCG 
TGCAGGCATG AGGTTGTCCT GGACAGACAC GGTGTCTACG GCCTGCAGCG AAACCTGCTA 
GTGGAGAACA TTATCGACAT TTACAAGCAG GAGTCATCCA GGCCGCTGCA CTCCAAGGCT 
GAGCAGCACC TCATGTGCGA GGAGCATGAA GAAGAGAAGA TCAATATTTA CTGCCTGAGC 
TGTGAGGTGC CCACCTGCTC TCTCTGCAAG GTCTTCGGTG CCCACAAGGA CTGTGAGGTG 
GCCCCACTGC CCACCATTTA CAAACGCCAG AAGAAACAGG ATCTCACTCT GTTGCCCAGG 
CTGGAGTGCA GTGGCACAAA CACAACTTAC TGCAGCCTTG ATCTCCCGAG CTCAAGTGAT 
CCTCCCATCT TAGCCTCGCA GAACACTAAG ATTATAGATA GTGAGCTCAG CGATGGCATC 
GCGATGCTGG TGGCAGGCAA TGACCGCGTG CAAGCAGTGA TCACACAGAT GGAGGAGGTG 
TGCCAGACTA TCGAGGACAA TAGCCGGAGG CAGAAGCAGT TGTTAAACCA GAGGTTTGAG 
AGCCTGTGCG CAGTGCTGGA GGAGCGCAAG GGTGAGCTGC TGCAGGCGCT GGCCCGGGAG 
CAAGAGGAGA AGCTGCAGCG CGTCCGCGGC CTCATCCGTC AGTATGGCGA CCACCTGGAG 
GCCTCCTCTA AGCTGGTGGA GTCTGCCATC CAGTCCATGG AAGAGCCACA AATGGCGCTG 
TATCTCCAGC AGGCCAAGGA GCTGATCAAT AAGGTCGGGG CCATGTCGAA GGTGGAGCTG 
GCAGGGCGGC CGGAGCCAGG CTATGAGAGC ATGGAGCAAT TCACCGTAAG GGTGGAGCAC 
GTGGCCGAAA TGCTGCGGAC CATCGACTTC CAGCCAGGCG CTTCCGGGGA GGAAGAGGAG 
GTGGCCCCAG ACGGAGAGGA GGGCAGCGCG GGGCCGGAGG AAGAGCGGCC GGATGGGCCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115935.3
CDS	304..1506
Translation	MNFTVGFKPLLGDAHSMDNLEKQLICPICLEMFSKPVVILPCQHNLCRKCANDVFQASNPLWQSRGSTTVSSGGRFRCPSCRHEVVLDRHGVYGLQRNLLVENIIDIYKQESSRPLHSKAEQHLMCEEHEEEKINIYCLSCEVPTCSLCKVFGAHKDCEVAPLPTIYKRQKKQDLTLLPRLECSGTNTTYCSLDLPSSSDPPILASQNTKIIDSELSDGIAMLVAGNDRVQAVITQMEEVCQTIEDNSRRQKQLLNQRFESLCAVLEERKGELLQALAREQEEKLQRVRGLIRQYGDHLEASSKLVESAIQSMEEPQMALYLQQAKELINKVGAMSKVELAGRPEPGYESMEQFTVRVEHVAEMLRTIDFQPGASGEEEEVAPDGEEGSAGPEEERPDGP

Target ORF information:

RefSeq Version	NM_032546.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032546.3

ORF Insert Sequence:

ATGAACTTCA CAGTGGGTTT CAAGCCGCTG CTAGGGGATG CACACAGCAT GGACAACCTG 
GAGAAGCAGC TCATCTGCCC CATCTGCCTG GAGATGTTCT CCAAACCAGT GGTGATCCTG 
CCCTGCCAAC ACAACCTGTG CCGCAAATGT GCCAACGACG TCTTCCAGGC CTCGAATCCT 
CTATGGCAGT CCCGGGGCTC CACCACTGTG TCTTCAGGAG GCCGTTTCCG CTGCCCATCG 
TGCAGGCATG AGGTTGTCCT GGACAGACAC GGTGTCTACG GCCTGCAGCG AAACCTGCTA 
GTGGAGAACA TTATCGACAT TTACAAGCAG GAGTCATCCA GGCCGCTGCA CTCCAAGGCT 
GAGCAGCACC TCATGTGCGA GGAGCATGAA GAAGAGAAGA TCAATATTTA CTGCCTGAGC 
TGTGAGGTGC CCACCTGCTC TCTCTGCAAG GTCTTCGGTG CCCACAAGGA CTGTGAGGTG 
GCCCCACTGC CCACCATTTA CAAACGCCAG AAGAAACAGG ATCTCACTCT GTTGCCCAGG 
CTGGAGTGCA GTGGCACAAA CACAACTTAC TGCAGCCTTG ATCTCCCGAG CTCAAGTGAT 
CCTCCCATCT TAGCCTCGCA GAACACTAAG ATTATAGATA GTGAGCTCAG CGATGGCATC 
GCGATGCTGG TGGCAGGCAA TGACCGCGTG CAAGCAGTGA TCACACAGAT GGAGGAGGTG 
TGCCAGACTA TCGAGGACAA TAGCCGGAGG CAGAAGCAGT TGTTAAACCA GAGGTTTGAG 
AGCCTGTGCG CAGTGCTGGA GGAGCGCAAG GGTGAGCTGC TGCAGGCGCT GGCCCGGGAG 
CAAGAGGAGA AGCTGCAGCG CGTCCGCGGC CTCATCCGTC AGTATGGCGA CCACCTGGAG 
GCCTCCTCTA AGCTGGTGGA GTCTGCCATC CAGTCCATGG AAGAGCCACA AATGGCGCTG 
TATCTCCAGC AGGCCAAGGA GCTGATCAAT AAGGTCGGGG CCATGTCGAA GGTGGAGCTG 
GCAGGGCGGC CGGAGCCAGG CTATGAGAGC ATGGAGCAAT TCACCGTAAG GGTGGAGCAC 
GTGGCCGAAA TGCTGCGGAC CATCGACTTC CAGCCAGGCG CTTCCGGGGA GGAAGAGGAG 
GTGGCCCCAG ACGGAGAGGA GGGCAGCGCG GGGCCGGAGG AAGAGCGGCC GGATGGGCCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM54 ( NM_187841.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_912730.2 Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55662
Clone ID Related Accession (Same CDS sequence)	NM_187841.2 , NM_187841.3 , XM_024453010.1
Accession Version	NM_187841.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 54 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA897004.1, BC141807.1 and CA423735.1. On Jul 30, 2010 this sequence version replaced NM_187841.1. Transcript Variant: This variant (2) lacks an in-frame segment within the coding region. The resulting shorter isoform (2) lacks an internal region, as compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: ERR279824.826.1, BC141807.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTTCA CAGTGGGTTT CAAGCCGCTG CTAGGGGATG CACACAGCAT GGACAACCTG 
GAGAAGCAGC TCATCTGCCC CATCTGCCTG GAGATGTTCT CCAAACCAGT GGTGATCCTG 
CCCTGCCAAC ACAACCTGTG CCGCAAATGT GCCAACGACG TCTTCCAGGC CTCGAATCCT 
CTATGGCAGT CCCGGGGCTC CACCACTGTG TCTTCAGGAG GCCGTTTCCG CTGCCCATCG 
TGCAGGCATG AGGTTGTCCT GGACAGACAC GGTGTCTACG GCCTGCAGCG AAACCTGCTA 
GTGGAGAACA TTATCGACAT TTACAAGCAG GAGTCATCCA GGCCGCTGCA CTCCAAGGCT 
GAGCAGCACC TCATGTGCGA GGAGCATGAA GAAGAGAAGA TCAATATTTA CTGCCTGAGC 
TGTGAGGTGC CCACCTGCTC TCTCTGCAAG GTCTTCGGTG CCCACAAGGA CTGTGAGGTG 
GCCCCACTGC CCACCATTTA CAAACGCCAG AAGAGTGAGC TCAGCGATGG CATCGCGATG 
CTGGTGGCAG GCAATGACCG CGTGCAAGCA GTGATCACAC AGATGGAGGA GGTGTGCCAG 
ACTATCGAGG ACAATAGCCG GAGGCAGAAG CAGTTGTTAA ACCAGAGGTT TGAGAGCCTG 
TGCGCAGTGC TGGAGGAGCG CAAGGGTGAG CTGCTGCAGG CGCTGGCCCG GGAGCAAGAG 
GAGAAGCTGC AGCGCGTCCG CGGCCTCATC CGTCAGTATG GCGACCACCT GGAGGCCTCC 
TCTAAGCTGG TGGAGTCTGC CATCCAGTCC ATGGAAGAGC CACAAATGGC GCTGTATCTC 
CAGCAGGCCA AGGAGCTGAT CAATAAGGTC GGGGCCATGT CGAAGGTGGA GCTGGCAGGG 
CGGCCGGAGC CAGGCTATGA GAGCATGGAG CAATTCACCG TAAGGGTGGA GCACGTGGCC 
GAAATGCTGC GGACCATCGA CTTCCAGCCA GGCGCTTCCG GGGAGGAAGA GGAGGTGGCC 
CCAGACGGAG AGGAGGGCAG CGCGGGGCCG GAGGAAGAGC GGCCGGATGG GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_912730.2
CDS	304..1380
Translation	MNFTVGFKPLLGDAHSMDNLEKQLICPICLEMFSKPVVILPCQHNLCRKCANDVFQASNPLWQSRGSTTVSSGGRFRCPSCRHEVVLDRHGVYGLQRNLLVENIIDIYKQESSRPLHSKAEQHLMCEEHEEEKINIYCLSCEVPTCSLCKVFGAHKDCEVAPLPTIYKRQKSELSDGIAMLVAGNDRVQAVITQMEEVCQTIEDNSRRQKQLLNQRFESLCAVLEERKGELLQALAREQEEKLQRVRGLIRQYGDHLEASSKLVESAIQSMEEPQMALYLQQAKELINKVGAMSKVELAGRPEPGYESMEQFTVRVEHVAEMLRTIDFQPGASGEEEEVAPDGEEGSAGPEEERPDGP

Target ORF information:

RefSeq Version	NM_187841.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_187841.2

ORF Insert Sequence:

ATGAACTTCA CAGTGGGTTT CAAGCCGCTG CTAGGGGATG CACACAGCAT GGACAACCTG 
GAGAAGCAGC TCATCTGCCC CATCTGCCTG GAGATGTTCT CCAAACCAGT GGTGATCCTG 
CCCTGCCAAC ACAACCTGTG CCGCAAATGT GCCAACGACG TCTTCCAGGC CTCGAATCCT 
CTATGGCAGT CCCGGGGCTC CACCACTGTG TCTTCAGGAG GCCGTTTCCG CTGCCCATCG 
TGCAGGCATG AGGTTGTCCT GGACAGACAC GGTGTCTACG GCCTGCAGCG AAACCTGCTA 
GTGGAGAACA TTATCGACAT TTACAAGCAG GAGTCATCCA GGCCGCTGCA CTCCAAGGCT 
GAGCAGCACC TCATGTGCGA GGAGCATGAA GAAGAGAAGA TCAATATTTA CTGCCTGAGC 
TGTGAGGTGC CCACCTGCTC TCTCTGCAAG GTCTTCGGTG CCCACAAGGA CTGTGAGGTG 
GCCCCACTGC CCACCATTTA CAAACGCCAG AAGAGTGAGC TCAGCGATGG CATCGCGATG 
CTGGTGGCAG GCAATGACCG CGTGCAAGCA GTGATCACAC AGATGGAGGA GGTGTGCCAG 
ACTATCGAGG ACAATAGCCG GAGGCAGAAG CAGTTGTTAA ACCAGAGGTT TGAGAGCCTG 
TGCGCAGTGC TGGAGGAGCG CAAGGGTGAG CTGCTGCAGG CGCTGGCCCG GGAGCAAGAG 
GAGAAGCTGC AGCGCGTCCG CGGCCTCATC CGTCAGTATG GCGACCACCT GGAGGCCTCC 
TCTAAGCTGG TGGAGTCTGC CATCCAGTCC ATGGAAGAGC CACAAATGGC GCTGTATCTC 
CAGCAGGCCA AGGAGCTGAT CAATAAGGTC GGGGCCATGT CGAAGGTGGA GCTGGCAGGG 
CGGCCGGAGC CAGGCTATGA GAGCATGGAG CAATTCACCG TAAGGGTGGA GCACGTGGCC 
GAAATGCTGC GGACCATCGA CTTCCAGCCA GGCGCTTCCG GGGAGGAAGA GGAGGTGGCC 
CCAGACGGAG AGGAGGGCAG CGCGGGGCCG GAGGAAGAGC GGCCGGATGG GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM54 ( NM_187841.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_912730.2 Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant 2, mRNA.

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CloneID	OHu55662
Clone ID Related Accession (Same CDS sequence)	NM_187841.2 , NM_187841.3 , XM_024453010.1
Accession Version	NM_187841.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 54 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA897004.1 and BC141807.1. On Jun 2, 2019 this sequence version replaced NM_187841.2. Transcript Variant: This variant (2) lacks an in-frame segment within the coding region. The resulting shorter isoform (2) lacks an internal region, as compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC141807.1, ERR279839.2774.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACTTCA CAGTGGGTTT CAAGCCGCTG CTAGGGGATG CACACAGCAT GGACAACCTG 
GAGAAGCAGC TCATCTGCCC CATCTGCCTG GAGATGTTCT CCAAACCAGT GGTGATCCTG 
CCCTGCCAAC ACAACCTGTG CCGCAAATGT GCCAACGACG TCTTCCAGGC CTCGAATCCT 
CTATGGCAGT CCCGGGGCTC CACCACTGTG TCTTCAGGAG GCCGTTTCCG CTGCCCATCG 
TGCAGGCATG AGGTTGTCCT GGACAGACAC GGTGTCTACG GCCTGCAGCG AAACCTGCTA 
GTGGAGAACA TTATCGACAT TTACAAGCAG GAGTCATCCA GGCCGCTGCA CTCCAAGGCT 
GAGCAGCACC TCATGTGCGA GGAGCATGAA GAAGAGAAGA TCAATATTTA CTGCCTGAGC 
TGTGAGGTGC CCACCTGCTC TCTCTGCAAG GTCTTCGGTG CCCACAAGGA CTGTGAGGTG 
GCCCCACTGC CCACCATTTA CAAACGCCAG AAGAGTGAGC TCAGCGATGG CATCGCGATG 
CTGGTGGCAG GCAATGACCG CGTGCAAGCA GTGATCACAC AGATGGAGGA GGTGTGCCAG 
ACTATCGAGG ACAATAGCCG GAGGCAGAAG CAGTTGTTAA ACCAGAGGTT TGAGAGCCTG 
TGCGCAGTGC TGGAGGAGCG CAAGGGTGAG CTGCTGCAGG CGCTGGCCCG GGAGCAAGAG 
GAGAAGCTGC AGCGCGTCCG CGGCCTCATC CGTCAGTATG GCGACCACCT GGAGGCCTCC 
TCTAAGCTGG TGGAGTCTGC CATCCAGTCC ATGGAAGAGC CACAAATGGC GCTGTATCTC 
CAGCAGGCCA AGGAGCTGAT CAATAAGGTC GGGGCCATGT CGAAGGTGGA GCTGGCAGGG 
CGGCCGGAGC CAGGCTATGA GAGCATGGAG CAATTCACCG TAAGGGTGGA GCACGTGGCC 
GAAATGCTGC GGACCATCGA CTTCCAGCCA GGCGCTTCCG GGGAGGAAGA GGAGGTGGCC 
CCAGACGGAG AGGAGGGCAG CGCGGGGCCG GAGGAAGAGC GGCCGGATGG GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_912730.2
CDS	304..1380
Translation	MNFTVGFKPLLGDAHSMDNLEKQLICPICLEMFSKPVVILPCQHNLCRKCANDVFQASNPLWQSRGSTTVSSGGRFRCPSCRHEVVLDRHGVYGLQRNLLVENIIDIYKQESSRPLHSKAEQHLMCEEHEEEKINIYCLSCEVPTCSLCKVFGAHKDCEVAPLPTIYKRQKSELSDGIAMLVAGNDRVQAVITQMEEVCQTIEDNSRRQKQLLNQRFESLCAVLEERKGELLQALAREQEEKLQRVRGLIRQYGDHLEASSKLVESAIQSMEEPQMALYLQQAKELINKVGAMSKVELAGRPEPGYESMEQFTVRVEHVAEMLRTIDFQPGASGEEEEVAPDGEEGSAGPEEERPDGP

Target ORF information:

RefSeq Version	NM_187841.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_187841.3

ORF Insert Sequence:

ATGAACTTCA CAGTGGGTTT CAAGCCGCTG CTAGGGGATG CACACAGCAT GGACAACCTG 
GAGAAGCAGC TCATCTGCCC CATCTGCCTG GAGATGTTCT CCAAACCAGT GGTGATCCTG 
CCCTGCCAAC ACAACCTGTG CCGCAAATGT GCCAACGACG TCTTCCAGGC CTCGAATCCT 
CTATGGCAGT CCCGGGGCTC CACCACTGTG TCTTCAGGAG GCCGTTTCCG CTGCCCATCG 
TGCAGGCATG AGGTTGTCCT GGACAGACAC GGTGTCTACG GCCTGCAGCG AAACCTGCTA 
GTGGAGAACA TTATCGACAT TTACAAGCAG GAGTCATCCA GGCCGCTGCA CTCCAAGGCT 
GAGCAGCACC TCATGTGCGA GGAGCATGAA GAAGAGAAGA TCAATATTTA CTGCCTGAGC 
TGTGAGGTGC CCACCTGCTC TCTCTGCAAG GTCTTCGGTG CCCACAAGGA CTGTGAGGTG 
GCCCCACTGC CCACCATTTA CAAACGCCAG AAGAGTGAGC TCAGCGATGG CATCGCGATG 
CTGGTGGCAG GCAATGACCG CGTGCAAGCA GTGATCACAC AGATGGAGGA GGTGTGCCAG 
ACTATCGAGG ACAATAGCCG GAGGCAGAAG CAGTTGTTAA ACCAGAGGTT TGAGAGCCTG 
TGCGCAGTGC TGGAGGAGCG CAAGGGTGAG CTGCTGCAGG CGCTGGCCCG GGAGCAAGAG 
GAGAAGCTGC AGCGCGTCCG CGGCCTCATC CGTCAGTATG GCGACCACCT GGAGGCCTCC 
TCTAAGCTGG TGGAGTCTGC CATCCAGTCC ATGGAAGAGC CACAAATGGC GCTGTATCTC 
CAGCAGGCCA AGGAGCTGAT CAATAAGGTC GGGGCCATGT CGAAGGTGGA GCTGGCAGGG 
CGGCCGGAGC CAGGCTATGA GAGCATGGAG CAATTCACCG TAAGGGTGGA GCACGTGGCC 
GAAATGCTGC GGACCATCGA CTTCCAGCCA GGCGCTTCCG GGGAGGAAGA GGAGGTGGCC 
CCAGACGGAG AGGAGGGCAG CGCGGGGCCG GAGGAAGAGC GGCCGGATGG GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM54 ( XM_024453010.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308778.1 Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant X1, mRNA.

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CloneID	OHu55662
Clone ID Related Accession (Same CDS sequence)	NM_187841.2 , NM_187841.3 , XM_024453010.1
Accession Version	XM_024453010.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 54 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTCA CAGTGGGTTT CAAGCCGCTG CTAGGGGATG CACACAGCAT GGACAACCTG 
GAGAAGCAGC TCATCTGCCC CATCTGCCTG GAGATGTTCT CCAAACCAGT GGTGATCCTG 
CCCTGCCAAC ACAACCTGTG CCGCAAATGT GCCAACGACG TCTTCCAGGC CTCGAATCCT 
CTATGGCAGT CCCGGGGCTC CACCACTGTG TCTTCAGGAG GCCGTTTCCG CTGCCCATCG 
TGCAGGCATG AGGTTGTCCT GGACAGACAC GGTGTCTACG GCCTGCAGCG AAACCTGCTA 
GTGGAGAACA TTATCGACAT TTACAAGCAG GAGTCATCCA GGCCGCTGCA CTCCAAGGCT 
GAGCAGCACC TCATGTGCGA GGAGCATGAA GAAGAGAAGA TCAATATTTA CTGCCTGAGC 
TGTGAGGTGC CCACCTGCTC TCTCTGCAAG GTCTTCGGTG CCCACAAGGA CTGTGAGGTG 
GCCCCACTGC CCACCATTTA CAAACGCCAG AAGAGTGAGC TCAGCGATGG CATCGCGATG 
CTGGTGGCAG GCAATGACCG CGTGCAAGCA GTGATCACAC AGATGGAGGA GGTGTGCCAG 
ACTATCGAGG ACAATAGCCG GAGGCAGAAG CAGTTGTTAA ACCAGAGGTT TGAGAGCCTG 
TGCGCAGTGC TGGAGGAGCG CAAGGGTGAG CTGCTGCAGG CGCTGGCCCG GGAGCAAGAG 
GAGAAGCTGC AGCGCGTCCG CGGCCTCATC CGTCAGTATG GCGACCACCT GGAGGCCTCC 
TCTAAGCTGG TGGAGTCTGC CATCCAGTCC ATGGAAGAGC CACAAATGGC GCTGTATCTC 
CAGCAGGCCA AGGAGCTGAT CAATAAGGTC GGGGCCATGT CGAAGGTGGA GCTGGCAGGG 
CGGCCGGAGC CAGGCTATGA GAGCATGGAG CAATTCACCG TAAGGGTGGA GCACGTGGCC 
GAAATGCTGC GGACCATCGA CTTCCAGCCA GGCGCTTCCG GGGAGGAAGA GGAGGTGGCC 
CCAGACGGAG AGGAGGGCAG CGCGGGGCCG GAGGAAGAGC GGCCGGATGG GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308778.1
CDS	304..1380
Translation	MNFTVGFKPLLGDAHSMDNLEKQLICPICLEMFSKPVVILPCQHNLCRKCANDVFQASNPLWQSRGSTTVSSGGRFRCPSCRHEVVLDRHGVYGLQRNLLVENIIDIYKQESSRPLHSKAEQHLMCEEHEEEKINIYCLSCEVPTCSLCKVFGAHKDCEVAPLPTIYKRQKSELSDGIAMLVAGNDRVQAVITQMEEVCQTIEDNSRRQKQLLNQRFESLCAVLEERKGELLQALAREQEEKLQRVRGLIRQYGDHLEASSKLVESAIQSMEEPQMALYLQQAKELINKVGAMSKVELAGRPEPGYESMEQFTVRVEHVAEMLRTIDFQPGASGEEEEVAPDGEEGSAGPEEERPDGP

Target ORF information:

RefSeq Version	XM_024453010.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453010.1

ORF Insert Sequence:

ATGAACTTCA CAGTGGGTTT CAAGCCGCTG CTAGGGGATG CACACAGCAT GGACAACCTG 
GAGAAGCAGC TCATCTGCCC CATCTGCCTG GAGATGTTCT CCAAACCAGT GGTGATCCTG 
CCCTGCCAAC ACAACCTGTG CCGCAAATGT GCCAACGACG TCTTCCAGGC CTCGAATCCT 
CTATGGCAGT CCCGGGGCTC CACCACTGTG TCTTCAGGAG GCCGTTTCCG CTGCCCATCG 
TGCAGGCATG AGGTTGTCCT GGACAGACAC GGTGTCTACG GCCTGCAGCG AAACCTGCTA 
GTGGAGAACA TTATCGACAT TTACAAGCAG GAGTCATCCA GGCCGCTGCA CTCCAAGGCT 
GAGCAGCACC TCATGTGCGA GGAGCATGAA GAAGAGAAGA TCAATATTTA CTGCCTGAGC 
TGTGAGGTGC CCACCTGCTC TCTCTGCAAG GTCTTCGGTG CCCACAAGGA CTGTGAGGTG 
GCCCCACTGC CCACCATTTA CAAACGCCAG AAGAGTGAGC TCAGCGATGG CATCGCGATG 
CTGGTGGCAG GCAATGACCG CGTGCAAGCA GTGATCACAC AGATGGAGGA GGTGTGCCAG 
ACTATCGAGG ACAATAGCCG GAGGCAGAAG CAGTTGTTAA ACCAGAGGTT TGAGAGCCTG 
TGCGCAGTGC TGGAGGAGCG CAAGGGTGAG CTGCTGCAGG CGCTGGCCCG GGAGCAAGAG 
GAGAAGCTGC AGCGCGTCCG CGGCCTCATC CGTCAGTATG GCGACCACCT GGAGGCCTCC 
TCTAAGCTGG TGGAGTCTGC CATCCAGTCC ATGGAAGAGC CACAAATGGC GCTGTATCTC 
CAGCAGGCCA AGGAGCTGAT CAATAAGGTC GGGGCCATGT CGAAGGTGGA GCTGGCAGGG 
CGGCCGGAGC CAGGCTATGA GAGCATGGAG CAATTCACCG TAAGGGTGGA GCACGTGGCC 
GAAATGCTGC GGACCATCGA CTTCCAGCCA GGCGCTTCCG GGGAGGAAGA GGAGGTGGCC 
CCAGACGGAG AGGAGGGCAG CGCGGGGCCG GAGGAAGAGC GGCCGGATGG GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM54 ( XM_024453011.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308779.1 Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu104924
Clone ID Related Accession (Same CDS sequence)	XM_024453011.1
Accession Version	XM_024453011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 54 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTCA CAGTGGGTTT CAAGCCGCTG CTAGGGGATG CACACAGCAT GGACAACCTG 
GAGAAGCAGC TCATCTGCCC CATCTGCCTG GAGATGTTCT CCAAACCAGT GGTGATCCTG 
CCCTGCCAAC ACAACCTGTG CCGCAAATGT GCCAACGACG TCTTCCAGGC CTCGAATCCT 
CTATGGCAGT CCCGGGGCTC CACCACTGTG TCTTCAGGAG GCCGTTTCCG CTGCCCATCG 
TGCAGGCATG AGGTTGTCCT GGACAGACAC GGTGTCTACG GCCTGCAGCG AAACCTGCTA 
GTGGAGAACA TTATCGACAT TTACAAGCAG GAGTCATCCA GGCCGCTGCA CTCCAAGGCT 
GAGCAGCACC TCATGTGCGA GGAGCATGAA GAAGAGAAGA TCAATATTTA CTGCCTGAGC 
TGTGAGGTGC CCACCTGCTC TCTCTGCAAG GTCTTCGGTG CCCACAAGGA CTGTGAGGTG 
GCCCCACTGC CCACCATTTA CAAACGCCAG AAGAGTGAGC TCAGCGATGG CATCGCGATG 
CTGGTGGCAG GCAATGACCG CGTGCAAGCA GTGATCACAC AGATGGAGGA GGTGTGCCAG 
ACTATCGAGG ACAATAGCCG GAGGCAGAAG CAGTTGTTAA ACCAGAGGTT TGAGAGCCTG 
TGCGCAGTGC TGGAGGAGCG CAAGGGTGAG CTGCTGCAGG CGCTGGCCCG GGAGCAAGAG 
GAGAAGCTGC AGCGCGTCCG CGGCCTCATC CGTCAGTATG GCGACCACCT GGAGGCCTCC 
TCTAAGCTGG TGGAGTCTGC CATCCAGTCC ATGGAAGAGC CACAAATGGC GCTGTATCTC 
CAGGCCAAGG AGCTGATCAA TAAGGTCGGG GCCATGTCGA AGGTGGAGCT GGCAGGGCGG 
CCGGAGCCAG GCTATGAGAG CATGGAGCAA TTCACCGTAA GGGTGGAGCA CGTGGCCGAA 
ATGCTGCGGA CCATCGACTT CCAGCCAGGC GCTTCCGGGG AGGAAGAGGA GGTGGCCCCA 
GACGGAGAGG AGGGCAGCGC GGGGCCGGAG GAAGAGCGGC CGGATGGGCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308779.1
CDS	304..1377
Translation	MNFTVGFKPLLGDAHSMDNLEKQLICPICLEMFSKPVVILPCQHNLCRKCANDVFQASNPLWQSRGSTTVSSGGRFRCPSCRHEVVLDRHGVYGLQRNLLVENIIDIYKQESSRPLHSKAEQHLMCEEHEEEKINIYCLSCEVPTCSLCKVFGAHKDCEVAPLPTIYKRQKSELSDGIAMLVAGNDRVQAVITQMEEVCQTIEDNSRRQKQLLNQRFESLCAVLEERKGELLQALAREQEEKLQRVRGLIRQYGDHLEASSKLVESAIQSMEEPQMALYLQAKELINKVGAMSKVELAGRPEPGYESMEQFTVRVEHVAEMLRTIDFQPGASGEEEEVAPDGEEGSAGPEEERPDGP

Target ORF information:

RefSeq Version	XM_024453011.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453011.1

ORF Insert Sequence:

ATGAACTTCA CAGTGGGTTT CAAGCCGCTG CTAGGGGATG CACACAGCAT GGACAACCTG 
GAGAAGCAGC TCATCTGCCC CATCTGCCTG GAGATGTTCT CCAAACCAGT GGTGATCCTG 
CCCTGCCAAC ACAACCTGTG CCGCAAATGT GCCAACGACG TCTTCCAGGC CTCGAATCCT 
CTATGGCAGT CCCGGGGCTC CACCACTGTG TCTTCAGGAG GCCGTTTCCG CTGCCCATCG 
TGCAGGCATG AGGTTGTCCT GGACAGACAC GGTGTCTACG GCCTGCAGCG AAACCTGCTA 
GTGGAGAACA TTATCGACAT TTACAAGCAG GAGTCATCCA GGCCGCTGCA CTCCAAGGCT 
GAGCAGCACC TCATGTGCGA GGAGCATGAA GAAGAGAAGA TCAATATTTA CTGCCTGAGC 
TGTGAGGTGC CCACCTGCTC TCTCTGCAAG GTCTTCGGTG CCCACAAGGA CTGTGAGGTG 
GCCCCACTGC CCACCATTTA CAAACGCCAG AAGAGTGAGC TCAGCGATGG CATCGCGATG 
CTGGTGGCAG GCAATGACCG CGTGCAAGCA GTGATCACAC AGATGGAGGA GGTGTGCCAG 
ACTATCGAGG ACAATAGCCG GAGGCAGAAG CAGTTGTTAA ACCAGAGGTT TGAGAGCCTG 
TGCGCAGTGC TGGAGGAGCG CAAGGGTGAG CTGCTGCAGG CGCTGGCCCG GGAGCAAGAG 
GAGAAGCTGC AGCGCGTCCG CGGCCTCATC CGTCAGTATG GCGACCACCT GGAGGCCTCC 
TCTAAGCTGG TGGAGTCTGC CATCCAGTCC ATGGAAGAGC CACAAATGGC GCTGTATCTC 
CAGGCCAAGG AGCTGATCAA TAAGGTCGGG GCCATGTCGA AGGTGGAGCT GGCAGGGCGG 
CCGGAGCCAG GCTATGAGAG CATGGAGCAA TTCACCGTAA GGGTGGAGCA CGTGGCCGAA 
ATGCTGCGGA CCATCGACTT CCAGCCAGGC GCTTCCGGGG AGGAAGAGGA GGTGGCCCCA 
GACGGAGAGG AGGGCAGCGC GGGGCCGGAG GAAGAGCGGC CGGATGGGCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM54 ( XM_024453012.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308780.1 Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu104925
Clone ID Related Accession (Same CDS sequence)	XM_024453012.1
Accession Version	XM_024453012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 54 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTTCA CAGTGGGTTT CAAGCCGCTG CTAGGGGATG CACACAGCAT GGACAACCTG 
GAGAAGCAGC TCATCTGCCC CATCTGCCTG GAGATGTTCT CCAAACCAGT GGTGATCCTG 
CCCTGCCAAC ACAACCTGTG CCGCAAATGT GCCAACGACG TCTTCCAGGC CTCGAATCCT 
CTATGGCAGT CCCGGGGCTC CACCACTGTG TCTTCAGGAG GCCGTTTCCG CTGCCCATCG 
TGCAGGCATG AGGTTGTCCT GGACAGACAC GGTGTCTACG GCCTGCAGCG AAACCTGCTA 
GTGGAGAACA TTATCGACAT TTACAAGCAG GAGTCATCCA GGCCGCTGCA CTCCAAGGCT 
GAGCAGCACC TCATGTGCGA GGAGCATGAA GAAGAGAAGA TCAATATTTA CTGCCTGAGC 
TGTGAGGTGC CCACCTGCTC TCTCTGCAAG GTCTTCGGTG CCCACAAGGA CTGTGAGGTG 
GCCCCACTGC CCACCATTTA CAAACGCCAG AAGGACAATA GCCGGAGGCA GAAGCAGTTG 
TTAAACCAGA GGTTTGAGAG CCTGTGCGCA GTGCTGGAGG AGCGCAAGGG TGAGCTGCTG 
CAGGCGCTGG CCCGGGAGCA AGAGGAGAAG CTGCAGCGCG TCCGCGGCCT CATCCGTCAG 
TATGGCGACC ACCTGGAGGC CTCCTCTAAG CTGGTGGAGT CTGCCATCCA GTCCATGGAA 
GAGCCACAAA TGGCGCTGTA TCTCCAGCAG GCCAAGGAGC TGATCAATAA GGTCGGGGCC 
ATGTCGAAGG TGGAGCTGGC AGGGCGGCCG GAGCCAGGCT ATGAGAGCAT GGAGCAATTC 
ACCGTAAGGG TGGAGCACGT GGCCGAAATG CTGCGGACCA TCGACTTCCA GCCAGGCGCT 
TCCGGGGAGG AAGAGGAGGT GGCCCCAGAC GGAGAGGAGG GCAGCGCGGG GCCGGAGGAA 
GAGCGGCCGG ATGGGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308780.1
CDS	304..1284
Translation	MNFTVGFKPLLGDAHSMDNLEKQLICPICLEMFSKPVVILPCQHNLCRKCANDVFQASNPLWQSRGSTTVSSGGRFRCPSCRHEVVLDRHGVYGLQRNLLVENIIDIYKQESSRPLHSKAEQHLMCEEHEEEKINIYCLSCEVPTCSLCKVFGAHKDCEVAPLPTIYKRQKDNSRRQKQLLNQRFESLCAVLEERKGELLQALAREQEEKLQRVRGLIRQYGDHLEASSKLVESAIQSMEEPQMALYLQQAKELINKVGAMSKVELAGRPEPGYESMEQFTVRVEHVAEMLRTIDFQPGASGEEEEVAPDGEEGSAGPEEERPDGP

Target ORF information:

RefSeq Version	XM_024453012.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 54 (TRIM54), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453012.1

ORF Insert Sequence:

ATGAACTTCA CAGTGGGTTT CAAGCCGCTG CTAGGGGATG CACACAGCAT GGACAACCTG 
GAGAAGCAGC TCATCTGCCC CATCTGCCTG GAGATGTTCT CCAAACCAGT GGTGATCCTG 
CCCTGCCAAC ACAACCTGTG CCGCAAATGT GCCAACGACG TCTTCCAGGC CTCGAATCCT 
CTATGGCAGT CCCGGGGCTC CACCACTGTG TCTTCAGGAG GCCGTTTCCG CTGCCCATCG 
TGCAGGCATG AGGTTGTCCT GGACAGACAC GGTGTCTACG GCCTGCAGCG AAACCTGCTA 
GTGGAGAACA TTATCGACAT TTACAAGCAG GAGTCATCCA GGCCGCTGCA CTCCAAGGCT 
GAGCAGCACC TCATGTGCGA GGAGCATGAA GAAGAGAAGA TCAATATTTA CTGCCTGAGC 
TGTGAGGTGC CCACCTGCTC TCTCTGCAAG GTCTTCGGTG CCCACAAGGA CTGTGAGGTG 
GCCCCACTGC CCACCATTTA CAAACGCCAG AAGGACAATA GCCGGAGGCA GAAGCAGTTG 
TTAAACCAGA GGTTTGAGAG CCTGTGCGCA GTGCTGGAGG AGCGCAAGGG TGAGCTGCTG 
CAGGCGCTGG CCCGGGAGCA AGAGGAGAAG CTGCAGCGCG TCCGCGGCCT CATCCGTCAG 
TATGGCGACC ACCTGGAGGC CTCCTCTAAG CTGGTGGAGT CTGCCATCCA GTCCATGGAA 
GAGCCACAAA TGGCGCTGTA TCTCCAGCAG GCCAAGGAGC TGATCAATAA GGTCGGGGCC 
ATGTCGAAGG TGGAGCTGGC AGGGCGGCCG GAGCCAGGCT ATGAGAGCAT GGAGCAATTC 
ACCGTAAGGG TGGAGCACGT GGCCGAAATG CTGCGGACCA TCGACTTCCA GCCAGGCGCT 
TCCGGGGAGG AAGAGGAGGT GGCCCCAGAC GGAGAGGAGG GCAGCGCGGG GCCGGAGGAA 
GAGCGGCCGG ATGGGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

New cardiac and skeletal protein aggregate myopathy associated with combined MuRF1 and MuRF3 mutations.
Human molecular genetics24(13)3638-50(2015 Jul)
Oliv? M,Abdul-Hussein S,Oldfors A,Gonz?lez-Costello J,van der Ven PF,F?rst DO,Gonz?lez L,Moreno D,Torrej?n-Escribano B,Ali? J,Pou A,Ferrer I,Tajsharghi H

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kov?cs IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barab?si AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M

Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain.
Journal of molecular biology306(4)717-26(2001 Mar)
Centner T,Yano J,Kimura E,McElhinny AS,Pelin K,Witt CC,Bang ML,Trombitas K,Granzier H,Gregorio CC,Sorimachi H,Labeit S

Regulation of microtubule dynamics and myogenic differentiation by MURF, a striated muscle RING-finger protein.
The Journal of cell biology150(4)771-84(2000 Aug)
Spencer JA,Eliazer S,Ilaria RL Jr,Richardson JA,Olson EN

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TRIM54 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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