ORF » Species Summary » Homo sapiens » ASAH2 cDNA ORF clone

ASAH2 cDNA ORF clone, Homo sapiens(human)

The following ASAH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASAH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu91542 XM_017016436.1
Latest version!		 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OHu19794 NM_019893.3
Latest version!		 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $629.30
$899.00
OHu19794 XM_011539970.2
Latest version!		 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $629.30
$899.00
OHu70070 XM_011539971.2
Latest version!		 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $391.30
$559.00
OHu70071 XM_011539972.3
Latest version!		 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $391.30
$559.00
OHu70072 XM_011539973.3
Latest version!		 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
OHu20060 NM_001143974.2
Latest version!		 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu91541 XM_017016435.1
Latest version!		 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu19794 NM_019893.2 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $629.30
$899.00
OHu70072 XM_011539973.2 Homo sapiens N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
OHu20060 NM_001143974.1 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu70071 XM_011539972.2 Homo sapiens N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $391.30
$559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    ASAH2 ( XM_017016436.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871925.1 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OHu91542
    Clone ID Related Accession (Same CDS sequence) XM_017016436.1
    Accession Version XM_017016436.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neutral ceramidase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAAGAATA CCAGGCTCAG CGATATGAGG CAGCATCGAC AATTTATGGA CCGCACACAT 
    TATCTGCTTA CATTCAGCTC TTCAGAAACC TTGCTAAGGC TATTGCTACG TATTGTGGAT 
    AGAGCACCAA AAGGCAGAAC TTTCGGGGAT GTCCTGCAGC CAGCAAAACC TGAATACAGA 
    GTGGGGGAAG TTGCTGAAGT TATATTTGTA GGTGCTAACC CGAAGAATTC AGTACAAAAC 
    CAGACCCATC AGACCTTCCT CACTGTGGAG AAATATGAGG CTACTTCAAC ATCGTGGCAG 
    ATAGTGTGTA ATGATGCCTC CTGGGAGACT CGTTTTTATT GGCACAAGGG ACTCCTGGGT 
    CTGAGTAATG CAACAGTGGA ATGGCATATT CCAGACACTG CCCAGCCTGG AATCTACAGA 
    ATAAGATATT TTGGACACAA TCGGAAGCAG GACATTCTGA AGCCTGCTGT CATACTTTCA 
    TTTGAAGGCA CTTCCCCGGC TTTTGAAGTT GTAACTATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016871925.1
    CDS81..602
    Translation

    Target ORF information:

    RefSeq Version XM_017016436.1
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017016436.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAAGAATA CCAGGCTCAG CGATATGAGG CAGCATCGAC AATTTATGGA CCGCACACAT 
    TATCTGCTTA CATTCAGCTC TTCAGAAACC TTGCTAAGGC TATTGCTACG TATTGTGGAT 
    AGAGCACCAA AAGGCAGAAC TTTCGGGGAT GTCCTGCAGC CAGCAAAACC TGAATACAGA 
    GTGGGGGAAG TTGCTGAAGT TATATTTGTA GGTGCTAACC CGAAGAATTC AGTACAAAAC 
    CAGACCCATC AGACCTTCCT CACTGTGGAG AAATATGAGG CTACTTCAAC ATCGTGGCAG 
    ATAGTGTGTA ATGATGCCTC CTGGGAGACT CGTTTTTATT GGCACAAGGG ACTCCTGGGT 
    CTGAGTAATG CAACAGTGGA ATGGCATATT CCAGACACTG CCCAGCCTGG AATCTACAGA 
    ATAAGATATT TTGGACACAA TCGGAAGCAG GACATTCTGA AGCCTGCTGT CATACTTTCA 
    TTTGAAGGCA CTTCCCCGGC TTTTGAAGTT GTAACTATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    ASAH2 ( NM_019893.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_063946.2 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu19794
    Clone ID Related Accession (Same CDS sequence) NM_019893.2 , XM_011539970.2 , NM_019893.3
    Accession Version NM_019893.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-10-05
    Organism Homo sapiens(Human)
    Product neutral ceramidase isoform a
    Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF449759.2, BC107105.2 and BG289059.1. On Jan 23, 2009 this sequence version replaced NM_019893.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF449759.2, AF250847.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGGAA CTCTATGCCT CTGCCTCCCA GGAGGTAACA 
    GGACCACTGG CTTCAGCACA CCAGTGGGTG GATATGACAG ATGTGACTGT CTGGCTCAAT 
    TCCACACATG CATCAAAAAC ATGTAAACCA GCATTGGGCT ACAGTTTTGC AGCTGGCACT 
    ATTGATGGAG TTGGAGGCCT CAATTTTACA CAGGGGAAAA CAGAAGGGGA TCCATTTTGG 
    GACACCATTC GGGACCAGAT CCTGGGAAAG CCATCTGAAG AAATTAAAGA ATGTCATAAA 
    CCAAAGCCCA TCCTTCTTCA CACCGGAGAA CTATCAAAAC CTCACCCCTG GCATCCAGAC 
    ATTGTTGATG TTCAGATTAT TACCCTTGGG TCCTTGGCCA TAACTGCCAT CCCCGGGGAG 
    TTTACGACCA TGTCTGGACG AAGACTTCGA GAGGCAGTTC AAGCAGAATT TGCATCTCAT 
    GGGATGCAGA ACATGACTGT TGTTATTTCA GGTCTATGCA ACGTCTATAC ACATTACATT 
    ACCACTTATG AAGAATACCA GGCTCAGCGA TATGAGGCAG CATCGACAAT TTATGGACCG 
    CACACATTAT CTGCTTACAT TCAGCTCTTC AGAAACCTTG CTAAGGCTAT TGCTACGGAC 
    ACGGTAGCCA ACCTGAGCAG AGGTCCAGAA CCTCCCTTTT TCAAACAATT AATAGTTCCA 
    TTAATTCCTA GTATTGTGGA TAGAGCACCA AAAGGCAGAA CTTTCGGGGA TGTCCTGCAG 
    CCAGCAAAAC CTGAATACAG AGTGGGGGAA GTTGCTGAAG TTATATTTGT AGGTGCTAAC 
    CCGAAGAATT CAGTACAAAA CCAGACCCAT CAGACCTTCC TCACTGTGGA GAAATATGAG 
    GCTACTTCAA CATCGTGGCA GATAGTGTGT AATGATGCCT CCTGGGAGAC TCGTTTTTAT 
    TGGCACAAGG GACTCCTGGG TCTGAGTAAT GCAACAGTGG AATGGCATAT TCCAGACACT 
    GCCCAGCCTG GAATCTACAG AATAAGATAT TTTGGACACA ATCGGAAGCA GGACATTCTG 
    AAGCCTGCTG TCATACTTTC ATTTGAAGGC ACTTCCCCGG CTTTTGAAGT TGTAACTATT 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_063946.2
    CDS1..2343
    Translation

    Target ORF information:

    RefSeq Version NM_019893.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_019893.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGGAA CTCTATGCCT CTGCCTCCCA GGAGGTAACA 
    GGACCACTGG CTTCAGCACA CCAGTGGGTG GATATGACAG ATGTGACTGT CTGGCTCAAT 
    TCCACACATG CATCAAAAAC ATGTAAACCA GCATTGGGCT ACAGTTTTGC AGCTGGCACT 
    ATTGATGGAG TTGGAGGCCT CAATTTTACA CAGGGGAAAA CAGAAGGGGA TCCATTTTGG 
    GACACCATTC GGGACCAGAT CCTGGGAAAG CCATCTGAAG AAATTAAAGA ATGTCATAAA 
    CCAAAGCCCA TCCTTCTTCA CACCGGAGAA CTATCAAAAC CTCACCCCTG GCATCCAGAC 
    ATTGTTGATG TTCAGATTAT TACCCTTGGG TCCTTGGCCA TAACTGCCAT CCCCGGGGAG 
    TTTACGACCA TGTCTGGACG AAGACTTCGA GAGGCAGTTC AAGCAGAATT TGCATCTCAT 
    GGGATGCAGA ACATGACTGT TGTTATTTCA GGTCTATGCA ACGTCTATAC ACATTACATT 
    ACCACTTATG AAGAATACCA GGCTCAGCGA TATGAGGCAG CATCGACAAT TTATGGACCG 
    CACACATTAT CTGCTTACAT TCAGCTCTTC AGAAACCTTG CTAAGGCTAT TGCTACGGAC 
    ACGGTAGCCA ACCTGAGCAG AGGTCCAGAA CCTCCCTTTT TCAAACAATT AATAGTTCCA 
    TTAATTCCTA GTATTGTGGA TAGAGCACCA AAAGGCAGAA CTTTCGGGGA TGTCCTGCAG 
    CCAGCAAAAC CTGAATACAG AGTGGGGGAA GTTGCTGAAG TTATATTTGT AGGTGCTAAC 
    CCGAAGAATT CAGTACAAAA CCAGACCCAT CAGACCTTCC TCACTGTGGA GAAATATGAG 
    GCTACTTCAA CATCGTGGCA GATAGTGTGT AATGATGCCT CCTGGGAGAC TCGTTTTTAT 
    TGGCACAAGG GACTCCTGGG TCTGAGTAAT GCAACAGTGG AATGGCATAT TCCAGACACT 
    GCCCAGCCTG GAATCTACAG AATAAGATAT TTTGGACACA ATCGGAAGCA GGACATTCTG 
    AAGCCTGCTG TCATACTTTC ATTTGAAGGC ACTTCCCCGG CTTTTGAAGT TGTAACTATT 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    ASAH2 ( NM_019893.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_063946.2 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu19794
    Clone ID Related Accession (Same CDS sequence) NM_019893.2 , XM_011539970.2 , NM_019893.3
    Accession Version NM_019893.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Homo sapiens(human)
    Product neutral ceramidase isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL450382.7 and N94907.1. On Aug 8, 2017 this sequence version replaced NM_019893.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF449759.2, AF250847.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGGAA CTCTATGCCT CTGCCTCCCA GGAGGTAACA 
    GGACCACTGG CTTCAGCACA CCAGTGGGTG GATATGACAG ATGTGACTGT CTGGCTCAAT 
    TCCACACATG CATCAAAAAC ATGTAAACCA GCATTGGGCT ACAGTTTTGC AGCTGGCACT 
    ATTGATGGAG TTGGAGGCCT CAATTTTACA CAGGGGAAAA CAGAAGGGGA TCCATTTTGG 
    GACACCATTC GGGACCAGAT CCTGGGAAAG CCATCTGAAG AAATTAAAGA ATGTCATAAA 
    CCAAAGCCCA TCCTTCTTCA CACCGGAGAA CTATCAAAAC CTCACCCCTG GCATCCAGAC 
    ATTGTTGATG TTCAGATTAT TACCCTTGGG TCCTTGGCCA TAACTGCCAT CCCCGGGGAG 
    TTTACGACCA TGTCTGGACG AAGACTTCGA GAGGCAGTTC AAGCAGAATT TGCATCTCAT 
    GGGATGCAGA ACATGACTGT TGTTATTTCA GGTCTATGCA ACGTCTATAC ACATTACATT 
    ACCACTTATG AAGAATACCA GGCTCAGCGA TATGAGGCAG CATCGACAAT TTATGGACCG 
    CACACATTAT CTGCTTACAT TCAGCTCTTC AGAAACCTTG CTAAGGCTAT TGCTACGGAC 
    ACGGTAGCCA ACCTGAGCAG AGGTCCAGAA CCTCCCTTTT TCAAACAATT AATAGTTCCA 
    TTAATTCCTA GTATTGTGGA TAGAGCACCA AAAGGCAGAA CTTTCGGGGA TGTCCTGCAG 
    CCAGCAAAAC CTGAATACAG AGTGGGGGAA GTTGCTGAAG TTATATTTGT AGGTGCTAAC 
    CCGAAGAATT CAGTACAAAA CCAGACCCAT CAGACCTTCC TCACTGTGGA GAAATATGAG 
    GCTACTTCAA CATCGTGGCA GATAGTGTGT AATGATGCCT CCTGGGAGAC TCGTTTTTAT 
    TGGCACAAGG GACTCCTGGG TCTGAGTAAT GCAACAGTGG AATGGCATAT TCCAGACACT 
    GCCCAGCCTG GAATCTACAG AATAAGATAT TTTGGACACA ATCGGAAGCA GGACATTCTG 
    AAGCCTGCTG TCATACTTTC ATTTGAAGGC ACTTCCCCGG CTTTTGAAGT TGTAACTATT 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_063946.2
    CDS1..2343
    Translation

    Target ORF information:

    RefSeq Version NM_019893.3
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_019893.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGGAA CTCTATGCCT CTGCCTCCCA GGAGGTAACA 
    GGACCACTGG CTTCAGCACA CCAGTGGGTG GATATGACAG ATGTGACTGT CTGGCTCAAT 
    TCCACACATG CATCAAAAAC ATGTAAACCA GCATTGGGCT ACAGTTTTGC AGCTGGCACT 
    ATTGATGGAG TTGGAGGCCT CAATTTTACA CAGGGGAAAA CAGAAGGGGA TCCATTTTGG 
    GACACCATTC GGGACCAGAT CCTGGGAAAG CCATCTGAAG AAATTAAAGA ATGTCATAAA 
    CCAAAGCCCA TCCTTCTTCA CACCGGAGAA CTATCAAAAC CTCACCCCTG GCATCCAGAC 
    ATTGTTGATG TTCAGATTAT TACCCTTGGG TCCTTGGCCA TAACTGCCAT CCCCGGGGAG 
    TTTACGACCA TGTCTGGACG AAGACTTCGA GAGGCAGTTC AAGCAGAATT TGCATCTCAT 
    GGGATGCAGA ACATGACTGT TGTTATTTCA GGTCTATGCA ACGTCTATAC ACATTACATT 
    ACCACTTATG AAGAATACCA GGCTCAGCGA TATGAGGCAG CATCGACAAT TTATGGACCG 
    CACACATTAT CTGCTTACAT TCAGCTCTTC AGAAACCTTG CTAAGGCTAT TGCTACGGAC 
    ACGGTAGCCA ACCTGAGCAG AGGTCCAGAA CCTCCCTTTT TCAAACAATT AATAGTTCCA 
    TTAATTCCTA GTATTGTGGA TAGAGCACCA AAAGGCAGAA CTTTCGGGGA TGTCCTGCAG 
    CCAGCAAAAC CTGAATACAG AGTGGGGGAA GTTGCTGAAG TTATATTTGT AGGTGCTAAC 
    CCGAAGAATT CAGTACAAAA CCAGACCCAT CAGACCTTCC TCACTGTGGA GAAATATGAG 
    GCTACTTCAA CATCGTGGCA GATAGTGTGT AATGATGCCT CCTGGGAGAC TCGTTTTTAT 
    TGGCACAAGG GACTCCTGGG TCTGAGTAAT GCAACAGTGG AATGGCATAT TCCAGACACT 
    GCCCAGCCTG GAATCTACAG AATAAGATAT TTTGGACACA ATCGGAAGCA GGACATTCTG 
    AAGCCTGCTG TCATACTTTC ATTTGAAGGC ACTTCCCCGG CTTTTGAAGT TGTAACTATT 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    ASAH2 ( XM_011539970.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538272.1 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OHu19794
    Clone ID Related Accession (Same CDS sequence) NM_019893.2 , XM_011539970.2 , NM_019893.3
    Accession Version XM_011539970.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neutral ceramidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539970.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGGAA CTCTATGCCT CTGCCTCCCA GGAGGTAACA 
    GGACCACTGG CTTCAGCACA CCAGTGGGTG GATATGACAG ATGTGACTGT CTGGCTCAAT 
    TCCACACATG CATCAAAAAC ATGTAAACCA GCATTGGGCT ACAGTTTTGC AGCTGGCACT 
    ATTGATGGAG TTGGAGGCCT CAATTTTACA CAGGGGAAAA CAGAAGGGGA TCCATTTTGG 
    GACACCATTC GGGACCAGAT CCTGGGAAAG CCATCTGAAG AAATTAAAGA ATGTCATAAA 
    CCAAAGCCCA TCCTTCTTCA CACCGGAGAA CTATCAAAAC CTCACCCCTG GCATCCAGAC 
    ATTGTTGATG TTCAGATTAT TACCCTTGGG TCCTTGGCCA TAACTGCCAT CCCCGGGGAG 
    TTTACGACCA TGTCTGGACG AAGACTTCGA GAGGCAGTTC AAGCAGAATT TGCATCTCAT 
    GGGATGCAGA ACATGACTGT TGTTATTTCA GGTCTATGCA ACGTCTATAC ACATTACATT 
    ACCACTTATG AAGAATACCA GGCTCAGCGA TATGAGGCAG CATCGACAAT TTATGGACCG 
    CACACATTAT CTGCTTACAT TCAGCTCTTC AGAAACCTTG CTAAGGCTAT TGCTACGGAC 
    ACGGTAGCCA ACCTGAGCAG AGGTCCAGAA CCTCCCTTTT TCAAACAATT AATAGTTCCA 
    TTAATTCCTA GTATTGTGGA TAGAGCACCA AAAGGCAGAA CTTTCGGGGA TGTCCTGCAG 
    CCAGCAAAAC CTGAATACAG AGTGGGGGAA GTTGCTGAAG TTATATTTGT AGGTGCTAAC 
    CCGAAGAATT CAGTACAAAA CCAGACCCAT CAGACCTTCC TCACTGTGGA GAAATATGAG 
    GCTACTTCAA CATCGTGGCA GATAGTGTGT AATGATGCCT CCTGGGAGAC TCGTTTTTAT 
    TGGCACAAGG GACTCCTGGG TCTGAGTAAT GCAACAGTGG AATGGCATAT TCCAGACACT 
    GCCCAGCCTG GAATCTACAG AATAAGATAT TTTGGACACA ATCGGAAGCA GGACATTCTG 
    AAGCCTGCTG TCATACTTTC ATTTGAAGGC ACTTCCCCGG CTTTTGAAGT TGTAACTATT 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538272.1
    CDS197..2539
    Translation

    Target ORF information:

    RefSeq Version XM_011539970.2
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011539970.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGGAA CTCTATGCCT CTGCCTCCCA GGAGGTAACA 
    GGACCACTGG CTTCAGCACA CCAGTGGGTG GATATGACAG ATGTGACTGT CTGGCTCAAT 
    TCCACACATG CATCAAAAAC ATGTAAACCA GCATTGGGCT ACAGTTTTGC AGCTGGCACT 
    ATTGATGGAG TTGGAGGCCT CAATTTTACA CAGGGGAAAA CAGAAGGGGA TCCATTTTGG 
    GACACCATTC GGGACCAGAT CCTGGGAAAG CCATCTGAAG AAATTAAAGA ATGTCATAAA 
    CCAAAGCCCA TCCTTCTTCA CACCGGAGAA CTATCAAAAC CTCACCCCTG GCATCCAGAC 
    ATTGTTGATG TTCAGATTAT TACCCTTGGG TCCTTGGCCA TAACTGCCAT CCCCGGGGAG 
    TTTACGACCA TGTCTGGACG AAGACTTCGA GAGGCAGTTC AAGCAGAATT TGCATCTCAT 
    GGGATGCAGA ACATGACTGT TGTTATTTCA GGTCTATGCA ACGTCTATAC ACATTACATT 
    ACCACTTATG AAGAATACCA GGCTCAGCGA TATGAGGCAG CATCGACAAT TTATGGACCG 
    CACACATTAT CTGCTTACAT TCAGCTCTTC AGAAACCTTG CTAAGGCTAT TGCTACGGAC 
    ACGGTAGCCA ACCTGAGCAG AGGTCCAGAA CCTCCCTTTT TCAAACAATT AATAGTTCCA 
    TTAATTCCTA GTATTGTGGA TAGAGCACCA AAAGGCAGAA CTTTCGGGGA TGTCCTGCAG 
    CCAGCAAAAC CTGAATACAG AGTGGGGGAA GTTGCTGAAG TTATATTTGT AGGTGCTAAC 
    CCGAAGAATT CAGTACAAAA CCAGACCCAT CAGACCTTCC TCACTGTGGA GAAATATGAG 
    GCTACTTCAA CATCGTGGCA GATAGTGTGT AATGATGCCT CCTGGGAGAC TCGTTTTTAT 
    TGGCACAAGG GACTCCTGGG TCTGAGTAAT GCAACAGTGG AATGGCATAT TCCAGACACT 
    GCCCAGCCTG GAATCTACAG AATAAGATAT TTTGGACACA ATCGGAAGCA GGACATTCTG 
    AAGCCTGCTG TCATACTTTC ATTTGAAGGC ACTTCCCCGG CTTTTGAAGT TGTAACTATT 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    ASAH2 ( XM_011539971.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538273.1 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OHu70070
    Clone ID Related Accession (Same CDS sequence) XM_011539971.2
    Accession Version XM_011539971.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neutral ceramidase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGGAA CTCTATGCCT CTGCCTCCCA GGAGGTAACA 
    GGACCACTGG CTTCAGCACA CCAGTGGGTG GATATGACAG ATGTGACTGT CTGGCTCAAT 
    TCCACACATG CATCAAAAAC ATGTAAACCA GCATTGGGCT ACAGTTTTGC AGCTGGCACT 
    ATTGATGGAG TTGGAGGCCT CAATTTTACA CAGGGGAAAA CAGAAGGGGA TCCATTTTGG 
    GACACCATTC GGGACCAGAT CCTGGGAAAG CCATCTGAAG AAATTAAAGA ATGTCATAAA 
    CCAAAGCCCA TCCTTCTTCA CACCGGAGAA CTATCAAAAC CTCACCCCTG GCATCCAGAC 
    ATTGTTGATG TTCAGATTAT TACCCTTGGG TCCTTGGCCA TAACTGCCAT CCCCGGGGAG 
    TTTACGACCA TGTCTGGACG AAGACTTCGA GAGGCAGTTC AAGCAGAATT TGCATCTCAT 
    GGGATGCAGA ACATGACTGT TGTTATTTCA GGTCTATGCA ACGTCTATAC ACATTACATT 
    ACCACTTATG AAGAATACCA GGCTCAGCGA TATGAGGCAG CATCGACAAT TTATGGACCG 
    CACACATTAT CTGCTTACAT TCAGCTCTTC AGAAACCTTG CTAAGGCTAT TGCTACGGGG 
    GAAGTTGCTG AAGTTATATT TGTAGGTGCT AACCCGAAGA ATTCAGTACA AAACCAGACC 
    CATCAGACCT TCCTCACTGT GGAGAAATAT GAGGCTACTT CAACATCGTG GCAGATAGTG 
    TGTAATGATG CCTCCTGGGA GACTCGTTTT TATTGGCACA AGGGACTCCT GGGTCTGAGT 
    AATGCAACAG TGGAATGGCA TATTCCAGAC ACTGCCCAGC CTGGAATCTA CAGAATAAGA 
    TATTTTGGAC ACAATCGGAA GCAGGACATT CTGAAGCCTG CTGTCATACT TTCATTTGAA 
    GGCACTTCCC CGGCTTTTGA AGTTGTAACT ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538273.1
    CDS201..2396
    Translation

    Target ORF information:

    RefSeq Version XM_011539971.2
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011539971.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGGAA CTCTATGCCT CTGCCTCCCA GGAGGTAACA 
    GGACCACTGG CTTCAGCACA CCAGTGGGTG GATATGACAG ATGTGACTGT CTGGCTCAAT 
    TCCACACATG CATCAAAAAC ATGTAAACCA GCATTGGGCT ACAGTTTTGC AGCTGGCACT 
    ATTGATGGAG TTGGAGGCCT CAATTTTACA CAGGGGAAAA CAGAAGGGGA TCCATTTTGG 
    GACACCATTC GGGACCAGAT CCTGGGAAAG CCATCTGAAG AAATTAAAGA ATGTCATAAA 
    CCAAAGCCCA TCCTTCTTCA CACCGGAGAA CTATCAAAAC CTCACCCCTG GCATCCAGAC 
    ATTGTTGATG TTCAGATTAT TACCCTTGGG TCCTTGGCCA TAACTGCCAT CCCCGGGGAG 
    TTTACGACCA TGTCTGGACG AAGACTTCGA GAGGCAGTTC AAGCAGAATT TGCATCTCAT 
    GGGATGCAGA ACATGACTGT TGTTATTTCA GGTCTATGCA ACGTCTATAC ACATTACATT 
    ACCACTTATG AAGAATACCA GGCTCAGCGA TATGAGGCAG CATCGACAAT TTATGGACCG 
    CACACATTAT CTGCTTACAT TCAGCTCTTC AGAAACCTTG CTAAGGCTAT TGCTACGGGG 
    GAAGTTGCTG AAGTTATATT TGTAGGTGCT AACCCGAAGA ATTCAGTACA AAACCAGACC 
    CATCAGACCT TCCTCACTGT GGAGAAATAT GAGGCTACTT CAACATCGTG GCAGATAGTG 
    TGTAATGATG CCTCCTGGGA GACTCGTTTT TATTGGCACA AGGGACTCCT GGGTCTGAGT 
    AATGCAACAG TGGAATGGCA TATTCCAGAC ACTGCCCAGC CTGGAATCTA CAGAATAAGA 
    TATTTTGGAC ACAATCGGAA GCAGGACATT CTGAAGCCTG CTGTCATACT TTCATTTGAA 
    GGCACTTCCC CGGCTTTTGA AGTTGTAACT ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    ASAH2 ( XM_011539972.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538274.1 Homo sapiens N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OHu70071
    Clone ID Related Accession (Same CDS sequence) XM_011539972.2 , XM_011539972.3
    Accession Version XM_011539972.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neutral ceramidase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG AGCATTGACA TAGCACACAC AAATATGAAA 
    CCAGGCAAAA TCTTCATCAA TAAAGGAAAT GTGGATGGTG TGCAGATCAA CAGAAGTCCG 
    TATTCTTACC TTCAAAATCC GCAGTCAGAG AGAGCAAGGT ATTCTTCAAA TACAGACAAG 
    GAAATGATAG TTTTGAAAAT GGTAGATTTG AATGGAGATG ACTTGGGCCT TATCAGCTGG 
    TTTGCCATCC ACCCGGTCAG CATGAACAAC AGTAACCATC TTGTAAACAG TGACAATGTG 
    GGCTATGCAT CTTACCTGCT TGAGCAAGAG AAGAACAAAG GATATCTACC TGGACAGGGG 
    CCATTTGTAG CAGCCTTTGC TTCATCAAAC CTAGGAGATG TGTCCCCCAA CATTCTTGGA 
    CCACGTTGCA TCAACACAGG AGAGTCCTGT GATAACGCCA ATAGCACTTG TCCCATTGGT 
    GGGCCTAGCA TGTGCATTGC TAAGGGACCT GGACAGGATA TGTTTGACAG CACACAAATT 
    ATAGGACGGG CCATGTATCA GAGAGCAAAG GAACTCTATG CCTCTGCCTC CCAGGAGGTA 
    ACAGGACCAC TGGCTTCAGC ACACCAGTGG GTGGATATGA CAGATGTGAC TGTCTGGCTC 
    AATTCCACAC ATGCATCAAA AACATGTAAA CCAGCATTGG GCTACAGTTT TGCAGCTGGC 
    ACTATTGATG GAGTTGGAGG CCTCAATTTT ACACAGGGGA AAACAGAAGG GGATCCATTT 
    TGGGACACCA TTCGGGACCA GATCCTGGGA AAGCCATCTG AAGAAATTAA AGAATGTCAT 
    AAACCAAAGC CCATCCTTCT TCACACCGGA GAACTATCAA AACCTCACCC CTGGCATCCA 
    GACATTGTTG ATGTTCAGAT TATTACCCTT GGGTCCTTGG CCATAACTGC CATCCCCGGG 
    GAGTTTACGA CCATGTCTGG ACGAAGACTT CGAGAGGCAG TTCAAGCAGA ATTTGCATCT 
    CATGGGATGC AGAACATGAC TGTTGTTATT TCAGGTCTAT GCAACGTCTA TACACATTAC 
    ATTACCACTT ATGAAGAATA CCAGGCTCAG CGATATGAGG CAGCATCGAC AATTTATGGA 
    CCGCACACAT TATCTGCTTA CATTCAGCTC TTCAGAAACC TTGCTAAGGC TATTGCTACG 
    GACACGGTAG CCAACCTGAG CAGAGGTCCA GAACCTCCCT TTTTCAAACA ATTAATAGTT 
    CCATTAATTC CTAGTATTGT GGATAGAGCA CCAAAAGGCA GAACTTTCGG GGATGTCCTG 
    CAGCCAGCAA AACCTGAATA CAGAGTGGGG GAAGTTGCTG AAGTTATATT TGTAGGTGCT 
    AACCCGAAGA ATTCAGTACA AAACCAGACC CATCAGACCT TCCTCACTGT GGAGAAATAT 
    GAGGCTACTT CAACATCGTG GCAGATAGTG TGTAATGATG CCTCCTGGGA GACTCGTTTT 
    TATTGGCACA AGGGACTCCT GGGTCTGAGT AATGCAACAG TGGAATGGCA TATTCCAGAC 
    ACTGCCCAGC CTGGAATCTA CAGAATAAGA TATTTTGGAC ACAATCGGAA GCAGGACATT 
    CTGAAGCCTG CTGTCATACT TTCATTTGAA GGCACTTCCC CGGCTTTTGA AGTTGTAACT 
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538274.1
    CDS198..2363
    Translation

    Target ORF information:

    RefSeq Version XM_011539972.2
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011539972.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG AGCATTGACA TAGCACACAC AAATATGAAA 
    CCAGGCAAAA TCTTCATCAA TAAAGGAAAT GTGGATGGTG TGCAGATCAA CAGAAGTCCG 
    TATTCTTACC TTCAAAATCC GCAGTCAGAG AGAGCAAGGT ATTCTTCAAA TACAGACAAG 
    GAAATGATAG TTTTGAAAAT GGTAGATTTG AATGGAGATG ACTTGGGCCT TATCAGCTGG 
    TTTGCCATCC ACCCGGTCAG CATGAACAAC AGTAACCATC TTGTAAACAG TGACAATGTG 
    GGCTATGCAT CTTACCTGCT TGAGCAAGAG AAGAACAAAG GATATCTACC TGGACAGGGG 
    CCATTTGTAG CAGCCTTTGC TTCATCAAAC CTAGGAGATG TGTCCCCCAA CATTCTTGGA 
    CCACGTTGCA TCAACACAGG AGAGTCCTGT GATAACGCCA ATAGCACTTG TCCCATTGGT 
    GGGCCTAGCA TGTGCATTGC TAAGGGACCT GGACAGGATA TGTTTGACAG CACACAAATT 
    ATAGGACGGG CCATGTATCA GAGAGCAAAG GAACTCTATG CCTCTGCCTC CCAGGAGGTA 
    ACAGGACCAC TGGCTTCAGC ACACCAGTGG GTGGATATGA CAGATGTGAC TGTCTGGCTC 
    AATTCCACAC ATGCATCAAA AACATGTAAA CCAGCATTGG GCTACAGTTT TGCAGCTGGC 
    ACTATTGATG GAGTTGGAGG CCTCAATTTT ACACAGGGGA AAACAGAAGG GGATCCATTT 
    TGGGACACCA TTCGGGACCA GATCCTGGGA AAGCCATCTG AAGAAATTAA AGAATGTCAT 
    AAACCAAAGC CCATCCTTCT TCACACCGGA GAACTATCAA AACCTCACCC CTGGCATCCA 
    GACATTGTTG ATGTTCAGAT TATTACCCTT GGGTCCTTGG CCATAACTGC CATCCCCGGG 
    GAGTTTACGA CCATGTCTGG ACGAAGACTT CGAGAGGCAG TTCAAGCAGA ATTTGCATCT 
    CATGGGATGC AGAACATGAC TGTTGTTATT TCAGGTCTAT GCAACGTCTA TACACATTAC 
    ATTACCACTT ATGAAGAATA CCAGGCTCAG CGATATGAGG CAGCATCGAC AATTTATGGA 
    CCGCACACAT TATCTGCTTA CATTCAGCTC TTCAGAAACC TTGCTAAGGC TATTGCTACG 
    GACACGGTAG CCAACCTGAG CAGAGGTCCA GAACCTCCCT TTTTCAAACA ATTAATAGTT 
    CCATTAATTC CTAGTATTGT GGATAGAGCA CCAAAAGGCA GAACTTTCGG GGATGTCCTG 
    CAGCCAGCAA AACCTGAATA CAGAGTGGGG GAAGTTGCTG AAGTTATATT TGTAGGTGCT 
    AACCCGAAGA ATTCAGTACA AAACCAGACC CATCAGACCT TCCTCACTGT GGAGAAATAT 
    GAGGCTACTT CAACATCGTG GCAGATAGTG TGTAATGATG CCTCCTGGGA GACTCGTTTT 
    TATTGGCACA AGGGACTCCT GGGTCTGAGT AATGCAACAG TGGAATGGCA TATTCCAGAC 
    ACTGCCCAGC CTGGAATCTA CAGAATAAGA TATTTTGGAC ACAATCGGAA GCAGGACATT 
    CTGAAGCCTG CTGTCATACT TTCATTTGAA GGCACTTCCC CGGCTTTTGA AGTTGTAACT 
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    ASAH2 ( XM_011539972.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538274.1 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OHu70071
    Clone ID Related Accession (Same CDS sequence) XM_011539972.2 , XM_011539972.3
    Accession Version XM_011539972.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neutral ceramidase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011539972.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG AGCATTGACA TAGCACACAC AAATATGAAA 
    CCAGGCAAAA TCTTCATCAA TAAAGGAAAT GTGGATGGTG TGCAGATCAA CAGAAGTCCG 
    TATTCTTACC TTCAAAATCC GCAGTCAGAG AGAGCAAGGT ATTCTTCAAA TACAGACAAG 
    GAAATGATAG TTTTGAAAAT GGTAGATTTG AATGGAGATG ACTTGGGCCT TATCAGCTGG 
    TTTGCCATCC ACCCGGTCAG CATGAACAAC AGTAACCATC TTGTAAACAG TGACAATGTG 
    GGCTATGCAT CTTACCTGCT TGAGCAAGAG AAGAACAAAG GATATCTACC TGGACAGGGG 
    CCATTTGTAG CAGCCTTTGC TTCATCAAAC CTAGGAGATG TGTCCCCCAA CATTCTTGGA 
    CCACGTTGCA TCAACACAGG AGAGTCCTGT GATAACGCCA ATAGCACTTG TCCCATTGGT 
    GGGCCTAGCA TGTGCATTGC TAAGGGACCT GGACAGGATA TGTTTGACAG CACACAAATT 
    ATAGGACGGG CCATGTATCA GAGAGCAAAG GAACTCTATG CCTCTGCCTC CCAGGAGGTA 
    ACAGGACCAC TGGCTTCAGC ACACCAGTGG GTGGATATGA CAGATGTGAC TGTCTGGCTC 
    AATTCCACAC ATGCATCAAA AACATGTAAA CCAGCATTGG GCTACAGTTT TGCAGCTGGC 
    ACTATTGATG GAGTTGGAGG CCTCAATTTT ACACAGGGGA AAACAGAAGG GGATCCATTT 
    TGGGACACCA TTCGGGACCA GATCCTGGGA AAGCCATCTG AAGAAATTAA AGAATGTCAT 
    AAACCAAAGC CCATCCTTCT TCACACCGGA GAACTATCAA AACCTCACCC CTGGCATCCA 
    GACATTGTTG ATGTTCAGAT TATTACCCTT GGGTCCTTGG CCATAACTGC CATCCCCGGG 
    GAGTTTACGA CCATGTCTGG ACGAAGACTT CGAGAGGCAG TTCAAGCAGA ATTTGCATCT 
    CATGGGATGC AGAACATGAC TGTTGTTATT TCAGGTCTAT GCAACGTCTA TACACATTAC 
    ATTACCACTT ATGAAGAATA CCAGGCTCAG CGATATGAGG CAGCATCGAC AATTTATGGA 
    CCGCACACAT TATCTGCTTA CATTCAGCTC TTCAGAAACC TTGCTAAGGC TATTGCTACG 
    GACACGGTAG CCAACCTGAG CAGAGGTCCA GAACCTCCCT TTTTCAAACA ATTAATAGTT 
    CCATTAATTC CTAGTATTGT GGATAGAGCA CCAAAAGGCA GAACTTTCGG GGATGTCCTG 
    CAGCCAGCAA AACCTGAATA CAGAGTGGGG GAAGTTGCTG AAGTTATATT TGTAGGTGCT 
    AACCCGAAGA ATTCAGTACA AAACCAGACC CATCAGACCT TCCTCACTGT GGAGAAATAT 
    GAGGCTACTT CAACATCGTG GCAGATAGTG TGTAATGATG CCTCCTGGGA GACTCGTTTT 
    TATTGGCACA AGGGACTCCT GGGTCTGAGT AATGCAACAG TGGAATGGCA TATTCCAGAC 
    ACTGCCCAGC CTGGAATCTA CAGAATAAGA TATTTTGGAC ACAATCGGAA GCAGGACATT 
    CTGAAGCCTG CTGTCATACT TTCATTTGAA GGCACTTCCC CGGCTTTTGA AGTTGTAACT 
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538274.1
    CDS199..2364
    Translation

    Target ORF information:

    RefSeq Version XM_011539972.3
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011539972.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG AGCATTGACA TAGCACACAC AAATATGAAA 
    CCAGGCAAAA TCTTCATCAA TAAAGGAAAT GTGGATGGTG TGCAGATCAA CAGAAGTCCG 
    TATTCTTACC TTCAAAATCC GCAGTCAGAG AGAGCAAGGT ATTCTTCAAA TACAGACAAG 
    GAAATGATAG TTTTGAAAAT GGTAGATTTG AATGGAGATG ACTTGGGCCT TATCAGCTGG 
    TTTGCCATCC ACCCGGTCAG CATGAACAAC AGTAACCATC TTGTAAACAG TGACAATGTG 
    GGCTATGCAT CTTACCTGCT TGAGCAAGAG AAGAACAAAG GATATCTACC TGGACAGGGG 
    CCATTTGTAG CAGCCTTTGC TTCATCAAAC CTAGGAGATG TGTCCCCCAA CATTCTTGGA 
    CCACGTTGCA TCAACACAGG AGAGTCCTGT GATAACGCCA ATAGCACTTG TCCCATTGGT 
    GGGCCTAGCA TGTGCATTGC TAAGGGACCT GGACAGGATA TGTTTGACAG CACACAAATT 
    ATAGGACGGG CCATGTATCA GAGAGCAAAG GAACTCTATG CCTCTGCCTC CCAGGAGGTA 
    ACAGGACCAC TGGCTTCAGC ACACCAGTGG GTGGATATGA CAGATGTGAC TGTCTGGCTC 
    AATTCCACAC ATGCATCAAA AACATGTAAA CCAGCATTGG GCTACAGTTT TGCAGCTGGC 
    ACTATTGATG GAGTTGGAGG CCTCAATTTT ACACAGGGGA AAACAGAAGG GGATCCATTT 
    TGGGACACCA TTCGGGACCA GATCCTGGGA AAGCCATCTG AAGAAATTAA AGAATGTCAT 
    AAACCAAAGC CCATCCTTCT TCACACCGGA GAACTATCAA AACCTCACCC CTGGCATCCA 
    GACATTGTTG ATGTTCAGAT TATTACCCTT GGGTCCTTGG CCATAACTGC CATCCCCGGG 
    GAGTTTACGA CCATGTCTGG ACGAAGACTT CGAGAGGCAG TTCAAGCAGA ATTTGCATCT 
    CATGGGATGC AGAACATGAC TGTTGTTATT TCAGGTCTAT GCAACGTCTA TACACATTAC 
    ATTACCACTT ATGAAGAATA CCAGGCTCAG CGATATGAGG CAGCATCGAC AATTTATGGA 
    CCGCACACAT TATCTGCTTA CATTCAGCTC TTCAGAAACC TTGCTAAGGC TATTGCTACG 
    GACACGGTAG CCAACCTGAG CAGAGGTCCA GAACCTCCCT TTTTCAAACA ATTAATAGTT 
    CCATTAATTC CTAGTATTGT GGATAGAGCA CCAAAAGGCA GAACTTTCGG GGATGTCCTG 
    CAGCCAGCAA AACCTGAATA CAGAGTGGGG GAAGTTGCTG AAGTTATATT TGTAGGTGCT 
    AACCCGAAGA ATTCAGTACA AAACCAGACC CATCAGACCT TCCTCACTGT GGAGAAATAT 
    GAGGCTACTT CAACATCGTG GCAGATAGTG TGTAATGATG CCTCCTGGGA GACTCGTTTT 
    TATTGGCACA AGGGACTCCT GGGTCTGAGT AATGCAACAG TGGAATGGCA TATTCCAGAC 
    ACTGCCCAGC CTGGAATCTA CAGAATAAGA TATTTTGGAC ACAATCGGAA GCAGGACATT 
    CTGAAGCCTG CTGTCATACT TTCATTTGAA GGCACTTCCC CGGCTTTTGA AGTTGTAACT 
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    ASAH2 ( XM_011539973.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538275.1 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OHu70072
    Clone ID Related Accession (Same CDS sequence) XM_011539973.3 , XM_011539973.2
    Accession Version XM_011539973.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neutral ceramidase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011539973.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGGAA CTCTATGCCT CTGCCTCCCA GGAGGTAACA 
    GGACCACTGG CTTCAGCACA CCAGTGGGTG GATATGACAG ATGTGACTGT CTGGCTCAAT 
    TCCACACATG CATCAAAAAC ATGTAAACCA GCATTGGGCT ACAGTTTTGC AGCTGGCACT 
    ATTGATGGAG TTGGAGGCCT CAATTTTACA CAGGGGAAAA CAGAAGGGGA TCCATTTTGG 
    GACACCATTC GGGACCAGAT CCTGGGAAAG CCATCTGAAG AAATTAAAGA ATGTCATAAA 
    CCAAAGCCCA TCCTTCTTCA CACCGGAGAA CTATCAAAAC CTCACCCCTG GCATCCAGAC 
    ATTGTTGATG TTCAGATTAT TACCCTTGGG TCCTTGGCCA TAACTGCCAT CCCCGGGGAG 
    TTTACGACCA TGTCTGGACG AAGACTTCGA GAGGCAGTTC AAGCAGAATT TGCATCTCAT 
    GGGATGCAGA ACATGACTGT TGTTATTTCA GGTCTATGCA ACGTCTATAC ACATTACATT 
    ACCACTTATG AAGAATACCA GGCTCAGCGA TATGAGGCAG CATCGACAAT TTATGGACCG 
    CACACATTAT CTGCTTACAT TCAGCTCTTC AGAAACCTTG CTAAGGCTAT TGCTACGGAA 
    CTTCACTTGT GGAAATCTTC CCTTTGGATG CAGTTGTTTA TGAAGCTGCA ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538275.1
    CDS207..2123
    Translation

    Target ORF information:

    RefSeq Version XM_011539973.3
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011539973.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGGAA CTCTATGCCT CTGCCTCCCA GGAGGTAACA 
    GGACCACTGG CTTCAGCACA CCAGTGGGTG GATATGACAG ATGTGACTGT CTGGCTCAAT 
    TCCACACATG CATCAAAAAC ATGTAAACCA GCATTGGGCT ACAGTTTTGC AGCTGGCACT 
    ATTGATGGAG TTGGAGGCCT CAATTTTACA CAGGGGAAAA CAGAAGGGGA TCCATTTTGG 
    GACACCATTC GGGACCAGAT CCTGGGAAAG CCATCTGAAG AAATTAAAGA ATGTCATAAA 
    CCAAAGCCCA TCCTTCTTCA CACCGGAGAA CTATCAAAAC CTCACCCCTG GCATCCAGAC 
    ATTGTTGATG TTCAGATTAT TACCCTTGGG TCCTTGGCCA TAACTGCCAT CCCCGGGGAG 
    TTTACGACCA TGTCTGGACG AAGACTTCGA GAGGCAGTTC AAGCAGAATT TGCATCTCAT 
    GGGATGCAGA ACATGACTGT TGTTATTTCA GGTCTATGCA ACGTCTATAC ACATTACATT 
    ACCACTTATG AAGAATACCA GGCTCAGCGA TATGAGGCAG CATCGACAAT TTATGGACCG 
    CACACATTAT CTGCTTACAT TCAGCTCTTC AGAAACCTTG CTAAGGCTAT TGCTACGGAA 
    CTTCACTTGT GGAAATCTTC CCTTTGGATG CAGTTGTTTA TGAAGCTGCA ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    ASAH2 ( XM_011539973.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538275.1 Homo sapiens N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OHu70072
    Clone ID Related Accession (Same CDS sequence) XM_011539973.3 , XM_011539973.2
    Accession Version XM_011539973.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neutral ceramidase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539973.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGGAA CTCTATGCCT CTGCCTCCCA GGAGGTAACA 
    GGACCACTGG CTTCAGCACA CCAGTGGGTG GATATGACAG ATGTGACTGT CTGGCTCAAT 
    TCCACACATG CATCAAAAAC ATGTAAACCA GCATTGGGCT ACAGTTTTGC AGCTGGCACT 
    ATTGATGGAG TTGGAGGCCT CAATTTTACA CAGGGGAAAA CAGAAGGGGA TCCATTTTGG 
    GACACCATTC GGGACCAGAT CCTGGGAAAG CCATCTGAAG AAATTAAAGA ATGTCATAAA 
    CCAAAGCCCA TCCTTCTTCA CACCGGAGAA CTATCAAAAC CTCACCCCTG GCATCCAGAC 
    ATTGTTGATG TTCAGATTAT TACCCTTGGG TCCTTGGCCA TAACTGCCAT CCCCGGGGAG 
    TTTACGACCA TGTCTGGACG AAGACTTCGA GAGGCAGTTC AAGCAGAATT TGCATCTCAT 
    GGGATGCAGA ACATGACTGT TGTTATTTCA GGTCTATGCA ACGTCTATAC ACATTACATT 
    ACCACTTATG AAGAATACCA GGCTCAGCGA TATGAGGCAG CATCGACAAT TTATGGACCG 
    CACACATTAT CTGCTTACAT TCAGCTCTTC AGAAACCTTG CTAAGGCTAT TGCTACGGAA 
    CTTCACTTGT GGAAATCTTC CCTTTGGATG CAGTTGTTTA TGAAGCTGCA ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538275.1
    CDS207..2123
    Translation

    Target ORF information:

    RefSeq Version XM_011539973.2
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011539973.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGGAA CTCTATGCCT CTGCCTCCCA GGAGGTAACA 
    GGACCACTGG CTTCAGCACA CCAGTGGGTG GATATGACAG ATGTGACTGT CTGGCTCAAT 
    TCCACACATG CATCAAAAAC ATGTAAACCA GCATTGGGCT ACAGTTTTGC AGCTGGCACT 
    ATTGATGGAG TTGGAGGCCT CAATTTTACA CAGGGGAAAA CAGAAGGGGA TCCATTTTGG 
    GACACCATTC GGGACCAGAT CCTGGGAAAG CCATCTGAAG AAATTAAAGA ATGTCATAAA 
    CCAAAGCCCA TCCTTCTTCA CACCGGAGAA CTATCAAAAC CTCACCCCTG GCATCCAGAC 
    ATTGTTGATG TTCAGATTAT TACCCTTGGG TCCTTGGCCA TAACTGCCAT CCCCGGGGAG 
    TTTACGACCA TGTCTGGACG AAGACTTCGA GAGGCAGTTC AAGCAGAATT TGCATCTCAT 
    GGGATGCAGA ACATGACTGT TGTTATTTCA GGTCTATGCA ACGTCTATAC ACATTACATT 
    ACCACTTATG AAGAATACCA GGCTCAGCGA TATGAGGCAG CATCGACAAT TTATGGACCG 
    CACACATTAT CTGCTTACAT TCAGCTCTTC AGAAACCTTG CTAAGGCTAT TGCTACGGAA 
    CTTCACTTGT GGAAATCTTC CCTTTGGATG CAGTTGTTTA TGAAGCTGCA ACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    ASAH2 ( NM_001143974.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001137446.1 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu20060
    Clone ID Related Accession (Same CDS sequence) NM_001143974.1 , NM_001143974.2
    Accession Version NM_001143974.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-10-05
    Organism Homo sapiens(Human)
    Product neutral ceramidase isoform b
    Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF449759.2, BC107105.2 and BG289059.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the internal coding region, compared to variant 1. The resulting isoform (b) is shorter, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGTCA AAAACATGTA AACCAGCATT GGGCTACAGT 
    TTTGCAGCTG GCACTATTGA TGGAGTTGGA GGCCTCAATT TTACACAGGG GAAAACAGAA 
    GGGGATCCAT TTTGGGACAC CATTCGGGAC CAGATCCTGG GAAAGCCATC TGAAGAAATT 
    AAAGAATGTC ATAAACCAAA GCCCATCCTT CTTCACACCG GAGAACTATC AAAACCTCAC 
    CCCTGGCATC CAGACATTGT TGATGTTCAG ATTATTACCC TTGGGTCCTT GGCCATAACT 
    GCCATCCCCG GGGAGTTTAC GACCATGTCT GGACGAAGAC TTCGAGAGGC AGTTCAAGCA 
    GAATTTGCAT CTCATGGGAT GCAGAACATG ACTGTTGTTA TTTCAGGTCT ATGCAACGTC 
    TATACACATT ACATTACCAC TTATGAAGAA TACCAGGCTC AGCGATATGA GGCAGCATCG 
    ACAATTTATG GACCGCACAC ATTATCTGCT TACATTCAGC TCTTCAGAAA CCTTGCTAAG 
    GCTATTGCTA CGGACACGGT AGCCAACCTG AGCAGAGGTC CAGAACCTCC CTTTTTCAAA 
    CAATTAATAG TTCCATTAAT TCCTAGTATT GTGGATAGAG CACCAAAAGG CAGAACTTTC 
    GGGGATGTCC TGCAGCCAGC AAAACCTGAA TACAGAGTGG GGGAAGTTGC TGAAGTTATA 
    TTTGTAGGTG CTAACCCGAA GAATTCAGTA CAAAACCAGA CCCATCAGAC CTTCCTCACT 
    GTGGAGAAAT ATGAGGCTAC TTCAACATCG TGGCAGATAG TGTGTAATGA TGCCTCCTGG 
    GAGACTCGTT TTTATTGGCA CAAGGGACTC CTGGGTCTGA GTAATGCAAC AGTGGAATGG 
    CATATTCCAG ACACTGCCCA GCCTGGAATC TACAGAATAA GATATTTTGG ACACAATCGG 
    AAGCAGGACA TTCTGAAGCC TGCTGTCATA CTTTCATTTG AAGGCACTTC CCCGGCTTTT 
    GAAGTTGTAA CTATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137446.1
    CDS1..2238
    Translation

    Target ORF information:

    RefSeq Version NM_001143974.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001143974.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGTCA AAAACATGTA AACCAGCATT GGGCTACAGT 
    TTTGCAGCTG GCACTATTGA TGGAGTTGGA GGCCTCAATT TTACACAGGG GAAAACAGAA 
    GGGGATCCAT TTTGGGACAC CATTCGGGAC CAGATCCTGG GAAAGCCATC TGAAGAAATT 
    AAAGAATGTC ATAAACCAAA GCCCATCCTT CTTCACACCG GAGAACTATC AAAACCTCAC 
    CCCTGGCATC CAGACATTGT TGATGTTCAG ATTATTACCC TTGGGTCCTT GGCCATAACT 
    GCCATCCCCG GGGAGTTTAC GACCATGTCT GGACGAAGAC TTCGAGAGGC AGTTCAAGCA 
    GAATTTGCAT CTCATGGGAT GCAGAACATG ACTGTTGTTA TTTCAGGTCT ATGCAACGTC 
    TATACACATT ACATTACCAC TTATGAAGAA TACCAGGCTC AGCGATATGA GGCAGCATCG 
    ACAATTTATG GACCGCACAC ATTATCTGCT TACATTCAGC TCTTCAGAAA CCTTGCTAAG 
    GCTATTGCTA CGGACACGGT AGCCAACCTG AGCAGAGGTC CAGAACCTCC CTTTTTCAAA 
    CAATTAATAG TTCCATTAAT TCCTAGTATT GTGGATAGAG CACCAAAAGG CAGAACTTTC 
    GGGGATGTCC TGCAGCCAGC AAAACCTGAA TACAGAGTGG GGGAAGTTGC TGAAGTTATA 
    TTTGTAGGTG CTAACCCGAA GAATTCAGTA CAAAACCAGA CCCATCAGAC CTTCCTCACT 
    GTGGAGAAAT ATGAGGCTAC TTCAACATCG TGGCAGATAG TGTGTAATGA TGCCTCCTGG 
    GAGACTCGTT TTTATTGGCA CAAGGGACTC CTGGGTCTGA GTAATGCAAC AGTGGAATGG 
    CATATTCCAG ACACTGCCCA GCCTGGAATC TACAGAATAA GATATTTTGG ACACAATCGG 
    AAGCAGGACA TTCTGAAGCC TGCTGTCATA CTTTCATTTG AAGGCACTTC CCCGGCTTTT 
    GAAGTTGTAA CTATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    ASAH2 ( NM_001143974.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001137446.1 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu20060
    Clone ID Related Accession (Same CDS sequence) NM_001143974.1 , NM_001143974.2
    Accession Version NM_001143974.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Homo sapiens(human)
    Product neutral ceramidase isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL450382.7 and N94907.1. On Aug 8, 2017 this sequence version replaced NM_001143974.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the internal coding region, compared to variant 1. The resulting isoform (b) is shorter, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC107105.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGTCA AAAACATGTA AACCAGCATT GGGCTACAGT 
    TTTGCAGCTG GCACTATTGA TGGAGTTGGA GGCCTCAATT TTACACAGGG GAAAACAGAA 
    GGGGATCCAT TTTGGGACAC CATTCGGGAC CAGATCCTGG GAAAGCCATC TGAAGAAATT 
    AAAGAATGTC ATAAACCAAA GCCCATCCTT CTTCACACCG GAGAACTATC AAAACCTCAC 
    CCCTGGCATC CAGACATTGT TGATGTTCAG ATTATTACCC TTGGGTCCTT GGCCATAACT 
    GCCATCCCCG GGGAGTTTAC GACCATGTCT GGACGAAGAC TTCGAGAGGC AGTTCAAGCA 
    GAATTTGCAT CTCATGGGAT GCAGAACATG ACTGTTGTTA TTTCAGGTCT ATGCAACGTC 
    TATACACATT ACATTACCAC TTATGAAGAA TACCAGGCTC AGCGATATGA GGCAGCATCG 
    ACAATTTATG GACCGCACAC ATTATCTGCT TACATTCAGC TCTTCAGAAA CCTTGCTAAG 
    GCTATTGCTA CGGACACGGT AGCCAACCTG AGCAGAGGTC CAGAACCTCC CTTTTTCAAA 
    CAATTAATAG TTCCATTAAT TCCTAGTATT GTGGATAGAG CACCAAAAGG CAGAACTTTC 
    GGGGATGTCC TGCAGCCAGC AAAACCTGAA TACAGAGTGG GGGAAGTTGC TGAAGTTATA 
    TTTGTAGGTG CTAACCCGAA GAATTCAGTA CAAAACCAGA CCCATCAGAC CTTCCTCACT 
    GTGGAGAAAT ATGAGGCTAC TTCAACATCG TGGCAGATAG TGTGTAATGA TGCCTCCTGG 
    GAGACTCGTT TTTATTGGCA CAAGGGACTC CTGGGTCTGA GTAATGCAAC AGTGGAATGG 
    CATATTCCAG ACACTGCCCA GCCTGGAATC TACAGAATAA GATATTTTGG ACACAATCGG 
    AAGCAGGACA TTCTGAAGCC TGCTGTCATA CTTTCATTTG AAGGCACTTC CCCGGCTTTT 
    GAAGTTGTAA CTATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137446.1
    CDS1..2238
    Translation

    Target ORF information:

    RefSeq Version NM_001143974.2
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001143974.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGTCA AAAACATGTA AACCAGCATT GGGCTACAGT 
    TTTGCAGCTG GCACTATTGA TGGAGTTGGA GGCCTCAATT TTACACAGGG GAAAACAGAA 
    GGGGATCCAT TTTGGGACAC CATTCGGGAC CAGATCCTGG GAAAGCCATC TGAAGAAATT 
    AAAGAATGTC ATAAACCAAA GCCCATCCTT CTTCACACCG GAGAACTATC AAAACCTCAC 
    CCCTGGCATC CAGACATTGT TGATGTTCAG ATTATTACCC TTGGGTCCTT GGCCATAACT 
    GCCATCCCCG GGGAGTTTAC GACCATGTCT GGACGAAGAC TTCGAGAGGC AGTTCAAGCA 
    GAATTTGCAT CTCATGGGAT GCAGAACATG ACTGTTGTTA TTTCAGGTCT ATGCAACGTC 
    TATACACATT ACATTACCAC TTATGAAGAA TACCAGGCTC AGCGATATGA GGCAGCATCG 
    ACAATTTATG GACCGCACAC ATTATCTGCT TACATTCAGC TCTTCAGAAA CCTTGCTAAG 
    GCTATTGCTA CGGACACGGT AGCCAACCTG AGCAGAGGTC CAGAACCTCC CTTTTTCAAA 
    CAATTAATAG TTCCATTAAT TCCTAGTATT GTGGATAGAG CACCAAAAGG CAGAACTTTC 
    GGGGATGTCC TGCAGCCAGC AAAACCTGAA TACAGAGTGG GGGAAGTTGC TGAAGTTATA 
    TTTGTAGGTG CTAACCCGAA GAATTCAGTA CAAAACCAGA CCCATCAGAC CTTCCTCACT 
    GTGGAGAAAT ATGAGGCTAC TTCAACATCG TGGCAGATAG TGTGTAATGA TGCCTCCTGG 
    GAGACTCGTT TTTATTGGCA CAAGGGACTC CTGGGTCTGA GTAATGCAAC AGTGGAATGG 
    CATATTCCAG ACACTGCCCA GCCTGGAATC TACAGAATAA GATATTTTGG ACACAATCGG 
    AAGCAGGACA TTCTGAAGCC TGCTGTCATA CTTTCATTTG AAGGCACTTC CCCGGCTTTT 
    GAAGTTGTAA CTATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    ASAH2 ( XM_017016435.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871924.1 Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OHu91541
    Clone ID Related Accession (Same CDS sequence) XM_017016435.1
    Accession Version XM_017016435.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2310bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neutral ceramidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGGAA CTCTATGCCT CTGCCTCCCA GGAGGTAACA 
    GGACCACTGG CTTCAGCACA CCAGTGGGTG GATATGACAG ATGTGACTGT CTGGCTCAAT 
    TCCACACATG CATCAAAAAC ATGTAAACCA GCATTGGGCT ACAGTTTTGC AGCTGGCACT 
    ATTGATGGAG TTGGAGGCCT CAATTTTACA CAGGGGAAAA CAGAAGGGGA TCCATTTTGG 
    GACACCATTC GGGACCAGAT CCTGGGAAAG CCATCTGAAG AAATTAAAGA ATGTCATAAA 
    CCAAAGCCCA TCCTTCTTCA CACCGGAGAA CTATCAAAAC CTCACCCCTG GCATCCAGAC 
    ATTGTTGATG TTCAGATTAT TACCCTTGGG TCCTTGGCCA TAACTGCCAT CCCCGGGGAG 
    TTTACGACCA TGTCTGGACG AAGACTTCGA GAGGCAGTTC AAGCAGAATT TGCATCTCAT 
    GGGATGCAGA ACATGACTGT TGTTATTTCA GGTCTATGCA ACGTCTATAC ACATTACATT 
    ACCACTTATG AAGAATACCA GGCTCAGCGA TATGAGGCAG CATCGACAAT TTATGGACCG 
    CACACATTAT CTGCTTACAT TCAGCTCTTC AGAAACCTTG CTAAGGCTAT TGCTACGCAC 
    AGACAAGAAT CTAGGAAAAA ACTTGGTACT CACTCCAGTA TTGTGGATAG AGCACCAAAA 
    GGCAGAACTT TCGGGGATGT CCTGCAGCCA GCAAAACCTG AATACAGAGT GGGGGAAGTT 
    GCTGAAGTTA TATTTGTAGG TGCTAACCCG AAGAATTCAG TACAAAACCA GACCCATCAG 
    ACCTTCCTCA CTGTGGAGAA ATATGAGGCT ACTTCAACAT CGTGGCAGAT AGTGTGTAAT 
    GATGCCTCCT GGGAGACTCG TTTTTATTGG CACAAGGGAC TCCTGGGTCT GAGTAATGCA 
    ACAGTGGAAT GGCATATTCC AGACACTGCC CAGCCTGGAA TCTACAGAAT AAGATATTTT 
    GGACACAATC GGAAGCAGGA CATTCTGAAG CCTGCTGTCA TACTTTCATT TGAAGGCACT 
    TCCCCGGCTT TTGAAGTTGT AACTATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016871924.1
    CDS201..2510
    Translation

    Target ORF information:

    RefSeq Version XM_017016435.1
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017016435.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCCAAAC GCACCTTCTC TAACTTGGAG ACATTCCTGA TTTTCCTCCT TGTAATGATG 
    AGTGCCATCA CAGTGGCCCT TCTCAGCCTC TTGTTTATCA CCAGTGGGAC CATTGAAAAC 
    CACAAAGATT TAGGAGGCCA TTTTTTTTCA ACCACCCAAA GCCCTCCAGC CACCCAGGGC 
    TCCACAGCTG CCCAACGCTC CACAGCCACC CAGCATTCCA CAGCCACCCA GAGCTCCACA 
    GCCACTCAAA CTTCTCCAGT GCCTTTAACC CCAGAGTCTC CTCTATTTCA GAACTTCAGT 
    GGCTACCATA TTGGTGTTGG ACGAGCTGAC TGCACAGGAC AAGTAGCAGA TATCAATTTG 
    ATGGGCTATG GCAAATCCGG CCAGAATGCA CAGGGCATCC TCACCAGGCT ATACAGTCGT 
    GCCTTCATCA TGGCAGAACC TGATGGGTCC AATCGAACAG TGTTTGTCAG CATCGACATA 
    GGCATGGTAT CACAAAGGCT CAGGCTGGAG GTCCTGAACA GACTGCAGAG TAAATATGGC 
    TCCCTGTACA GAAGAGATAA TGTCATCCTG AGTGGCACTC ACACTCATTC AGGTCCTGCA 
    GGATATTTCC AGTATACCGT GTTTGTAATT GCCAGTGAAG GATTTAGCAA TCAAACTTTT 
    CAGCACATGG TCACTGGTAT CTTGAAGAGC ATTGACATAG CACACACAAA TATGAAACCA 
    GGCAAAATCT TCATCAATAA AGGAAATGTG GATGGTGTGC AGATCAACAG AAGTCCGTAT 
    TCTTACCTTC AAAATCCGCA GTCAGAGAGA GCAAGGTATT CTTCAAATAC AGACAAGGAA 
    ATGATAGTTT TGAAAATGGT AGATTTGAAT GGAGATGACT TGGGCCTTAT CAGCTGGTTT 
    GCCATCCACC CGGTCAGCAT GAACAACAGT AACCATCTTG TAAACAGTGA CAATGTGGGC 
    TATGCATCTT ACCTGCTTGA GCAAGAGAAG AACAAAGGAT ATCTACCTGG ACAGGGGCCA 
    TTTGTAGCAG CCTTTGCTTC ATCAAACCTA GGAGATGTGT CCCCCAACAT TCTTGGACCA 
    CGTTGCATCA ACACAGGAGA GTCCTGTGAT AACGCCAATA GCACTTGTCC CATTGGTGGG 
    CCTAGCATGT GCATTGCTAA GGGACCTGGA CAGGATATGT TTGACAGCAC ACAAATTATA 
    GGACGGGCCA TGTATCAGAG AGCAAAGGAA CTCTATGCCT CTGCCTCCCA GGAGGTAACA 
    GGACCACTGG CTTCAGCACA CCAGTGGGTG GATATGACAG ATGTGACTGT CTGGCTCAAT 
    TCCACACATG CATCAAAAAC ATGTAAACCA GCATTGGGCT ACAGTTTTGC AGCTGGCACT 
    ATTGATGGAG TTGGAGGCCT CAATTTTACA CAGGGGAAAA CAGAAGGGGA TCCATTTTGG 
    GACACCATTC GGGACCAGAT CCTGGGAAAG CCATCTGAAG AAATTAAAGA ATGTCATAAA 
    CCAAAGCCCA TCCTTCTTCA CACCGGAGAA CTATCAAAAC CTCACCCCTG GCATCCAGAC 
    ATTGTTGATG TTCAGATTAT TACCCTTGGG TCCTTGGCCA TAACTGCCAT CCCCGGGGAG 
    TTTACGACCA TGTCTGGACG AAGACTTCGA GAGGCAGTTC AAGCAGAATT TGCATCTCAT 
    GGGATGCAGA ACATGACTGT TGTTATTTCA GGTCTATGCA ACGTCTATAC ACATTACATT 
    ACCACTTATG AAGAATACCA GGCTCAGCGA TATGAGGCAG CATCGACAAT TTATGGACCG 
    CACACATTAT CTGCTTACAT TCAGCTCTTC AGAAACCTTG CTAAGGCTAT TGCTACGCAC 
    AGACAAGAAT CTAGGAAAAA ACTTGGTACT CACTCCAGTA TTGTGGATAG AGCACCAAAA 
    GGCAGAACTT TCGGGGATGT CCTGCAGCCA GCAAAACCTG AATACAGAGT GGGGGAAGTT 
    GCTGAAGTTA TATTTGTAGG TGCTAACCCG AAGAATTCAG TACAAAACCA GACCCATCAG 
    ACCTTCCTCA CTGTGGAGAA ATATGAGGCT ACTTCAACAT CGTGGCAGAT AGTGTGTAAT 
    GATGCCTCCT GGGAGACTCG TTTTTATTGG CACAAGGGAC TCCTGGGTCT GAGTAATGCA 
    ACAGTGGAAT GGCATATTCC AGACACTGCC CAGCCTGGAA TCTACAGAAT AAGATATTTT 
    GGACACAATC GGAAGCAGGA CATTCTGAAG CCTGCTGTCA TACTTTCATT TGAAGGCACT 
    TCCCCGGCTT TTGAAGTTGT AACTATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文