NSMCE3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSMCE3
Entrez Gene ID	56160
Full Name	NSE3 homolog, SMC5-SMC6 complex component
Synonyms	HCA4,LICS,MAGEG1,MAGEL3,NDNL2,NSE3
General protein information	Preferred Names NSE3 homolog, SMC5-SMC6 complex component Names non-structural maintenance of chromosomes element 3 homolog MAGE-G1 antigen hepatocellular carcinoma-associated protein 4 hepatocellular carcinoma-associated protein HCA4 melanoma antigen, family G, 1 melanoma-associated antigen G1 necdin-like 2, SMC5-SMC6 complex component necdin-like gene 2 necdin-like protein 2 non-SMC element 3 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q13.1
Summary	The protein encoded by this gene is part of the SMC5-6 chromatin reorganizing complex and is a member of the MAGE superfamily. This is an intronless gene. [provided by RefSeq, May 2011]. COMPLETENESS: complete on the 3' end.
Disorder MIM:	608243
Disorder Html:	Lung disease, immunodeficiency, and chromosome breakage syndrome, 617241 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_138704.3	NP_619649.1	non-structural maintenance of chromosomes element 3 homolog
NM_138704.4	NP_619649.1	non-structural maintenance of chromosomes element 3 homolog

Pathways from BioSystems

Homologies

Homo sapiens (human)	NDNL2	NP_619649.1
Pan troglodytes (chimpanzee)	NDNL2	XP_001163393.1
Bos taurus (cattle)	NDNL2	NP_001071548.1
Rattus norvegicus (Norway rat)	Ndnl2	NP_001166998.1
Xenopus tropicalis (tropical clawed frog)	ndnl2	NP_001016930.1
	NDNL2	XP_001109764.1
Canis lupus familiaris (dog)	NDNL2	XP_005618335.1
Gallus gallus (chicken)	NDNL2	NP_001098534.1
Mus musculus (house mouse)	Ndnl2	NP_075728.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Destabilized SMC5/6 complex leads to chromosome breakage syndrome with severe lung disease.
van der Crabben SN, Hennus MP, McGregor GA, Ritter DI, Nagamani SC, Wells OS, Harakalova M, Chinn IK, Alt A, Vondrova L, Hochstenbach R, van Montfrans JM, Terheggen-Lagro SW, van Lieshout S, van Roosmalen MJ, Renkens I, Duran K, Nijman IJ, Kloosterman WP, Hennekam E, Orange JS, van Hasselt PM, Wheeler DA, Palecek JJ, Lehmann AR, Oliver AW, Pearl LH, Plon SE, Murray JM, van Haaften G
The Journal of clinical investigation126(8)2881-92(2016 Aug)

Interactions between the Nse3 and Nse4 components of the SMC5-6 complex identify evolutionarily conserved interactions between MAGE and EID Families.
Hudson JJ, Bednarova K, Kozakova L, Liao C, Guerineau M, Colnaghi R, Vidot S, Marek J, Bathula SR, Lehmann AR, Palecek J
PloS one6(2)e17270(2011 Feb)

MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.
Doyle JM, Gao J, Wang J, Yang M, Potts PR
Molecular cell39(6)963-74(2010 Sep)

Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex.
Taylor EM, Copsey AC, Hudson JJ, Vidot S, Lehmann AR
Molecular and cellular biology28(4)1197-206(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NSMCE3 (also known as NDNL2) gene encodes a subunit of the SMC5/6 complex that is essential for DNA damage response and chromosome segregation
Title: Destabilized SMC5/6 complex leads to chromosome breakage syndrome with severe lung disease.

The four non-SMC components of the human complex were identified and characterized and it was demonstrated that the MAGEG1 protein is part of this complex.
Title: Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex.

gene expression, imprinting, and chromosome mapping of NDNL2
Title: A necdin/MAGE-like gene in the chromosome 15 autism susceptibility region: expression, imprinting, and mapping of the human and mouse orthologues.

The following NSMCE3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSMCE3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55633	NM_138704.4 Latest version!	Homo sapiens NSE3 homolog, SMC5-SMC6 complex component (NSMCE3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu55633	NM_138704.3	Homo sapiens NSE3 homolog, SMC5-SMC6 complex component (NSMCE3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSMCE3 ( NM_138704.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_619649.1 Homo sapiens NSE3 homolog, SMC5-SMC6 complex component (NSMCE3), mRNA.

Price & Availability↑

CloneID	OHu55633
Clone ID Related Accession (Same CDS sequence)	NM_138704.3 , NM_138704.4
Accession Version	NM_138704.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	non-structural maintenance of chromosomes element 3 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU310582.1 and BC053999.1. This sequence is a reference standard in the RefSeqGene project. On May 4, 2011 this sequence version replaced NM_138704.2. COMPLETENESS: complete on the 3' end.

ATGTTGCAAA AACCGAGGAA CCGGGGCCGC TCTGGCGGCC AGGCCGAGAG GGACAGAGAC 
TGGAGCCATA GCGGAAACCC CGGGGCTTCG CGGGCCGGGG AAGACGCCCG GGTTCTCAGA 
GACGGCTTTG CCGAGGAGGC CCCGAGCACG TCCCGCGGGC CGGGCGGCTC GCAGGGGTCG 
CAGGGCCCCT CGCCTCAGGG CGCCCGCCGG GCCCAGGCCG CCCCCGCCGT GGGGCCCAGG 
AGCCAGAAGC AGCTGGAGCT GAAAGTGTCC GAGCTGGTGC AGTTCTTGCT GATTAAAGAC 
CAGAAGAAGA TTCCGATCAA GCGGGCCGAC ATACTGAAGC ACGTCATCGG GGACTACAAG 
GACATCTTCC CCGACCTCTT CAAACGGGCC GCCGAGCGCC TCCAGTACGT CTTCGGGTAT 
AAGCTGGTGG AACTTGAACC CAAGAGCAAC ACTTACATCC TCATCAACAC CCTGGAGCCT 
GTGGAGGAGG ATGCCGAGAT GAGGGGTGAC CAAGGCACGC CCACTACGGG CCTCCTGATG 
ATCGTCTTAG GGCTCATCTT TATGAAGGGC AACACCATCA AGGAAACTGA AGCCTGGGAC 
TTTCTGCGGC GCTTAGGGGT CTACCCCACC AAGAAGCATT TAATTTTCGG AGATCCAAAG 
AAACTCATTA CTGAGGACTT TGTGCGACAG CGTTACCTGG AATACCGGCG GATACCCCAC 
ACCGACCCCG TCGACTACGA ATTCCAGTGG GGCCCGCGAA CCAACCTGGA AACCAGCAAG 
ATGAAAGTTC TTAAGTTTGT GGCCAAGGTC CATAATCAAG ACCCCAAGGA CTGGCCAGCG 
CAGTACTGTG AGGCTTTGGC AGATGAGGAG AACAGGGCCA GACCTCAGCC TAGTGGCCCA 
GCTCCATCCT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619649.1
CDS	112..1026
Translation	MLQKPRNRGRSGGQAERDRDWSHSGNPGASRAGEDARVLRDGFAEEAPSTSRGPGGSQGSQGPSPQGARRAQAAPAVGPRSQKQLELKVSELVQFLLIKDQKKIPIKRADILKHVIGDYKDIFPDLFKRAAERLQYVFGYKLVELEPKSNTYILINTLEPVEEDAEMRGDQGTPTTGLLMIVLGLIFMKGNTIKETEAWDFLRRLGVYPTKKHLIFGDPKKLITEDFVRQRYLEYRRIPHTDPVDYEFQWGPRTNLETSKMKVLKFVAKVHNQDPKDWPAQYCEALADEENRARPQPSGPAPSS

Target ORF information:

RefSeq Version	NM_138704.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NSE3 homolog, SMC5-SMC6 complex component (NSMCE3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138704.3

ORF Insert Sequence:

ATGTTGCAAA AACCGAGGAA CCGGGGCCGC TCTGGCGGCC AGGCCGAGAG GGACAGAGAC 
TGGAGCCATA GCGGAAACCC CGGGGCTTCG CGGGCCGGGG AAGACGCCCG GGTTCTCAGA 
GACGGCTTTG CCGAGGAGGC CCCGAGCACG TCCCGCGGGC CGGGCGGCTC GCAGGGGTCG 
CAGGGCCCCT CGCCTCAGGG CGCCCGCCGG GCCCAGGCCG CCCCCGCCGT GGGGCCCAGG 
AGCCAGAAGC AGCTGGAGCT GAAAGTGTCC GAGCTGGTGC AGTTCTTGCT GATTAAAGAC 
CAGAAGAAGA TTCCGATCAA GCGGGCCGAC ATACTGAAGC ACGTCATCGG GGACTACAAG 
GACATCTTCC CCGACCTCTT CAAACGGGCC GCCGAGCGCC TCCAGTACGT CTTCGGGTAT 
AAGCTGGTGG AACTTGAACC CAAGAGCAAC ACTTACATCC TCATCAACAC CCTGGAGCCT 
GTGGAGGAGG ATGCCGAGAT GAGGGGTGAC CAAGGCACGC CCACTACGGG CCTCCTGATG 
ATCGTCTTAG GGCTCATCTT TATGAAGGGC AACACCATCA AGGAAACTGA AGCCTGGGAC 
TTTCTGCGGC GCTTAGGGGT CTACCCCACC AAGAAGCATT TAATTTTCGG AGATCCAAAG 
AAACTCATTA CTGAGGACTT TGTGCGACAG CGTTACCTGG AATACCGGCG GATACCCCAC 
ACCGACCCCG TCGACTACGA ATTCCAGTGG GGCCCGCGAA CCAACCTGGA AACCAGCAAG 
ATGAAAGTTC TTAAGTTTGT GGCCAAGGTC CATAATCAAG ACCCCAAGGA CTGGCCAGCG 
CAGTACTGTG AGGCTTTGGC AGATGAGGAG AACAGGGCCA GACCTCAGCC TAGTGGCCCA 
GCTCCATCCT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSMCE3 ( NM_138704.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_619649.1 Homo sapiens NSE3 homolog, SMC5-SMC6 complex component (NSMCE3), mRNA.

Price & Availability↑

CloneID	OHu55633
Clone ID Related Accession (Same CDS sequence)	NM_138704.3 , NM_138704.4
Accession Version	NM_138704.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	non-structural maintenance of chromosomes element 3 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC061965.16, AU310582.1 and BC053999.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_138704.3. ##Evidence-Data-START## Transcript is intronless :: SRR1660805.354412.1, DRR138512.517665.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000332303.6/ ENSP00000330694.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTGCAAA AACCGAGGAA CCGGGGCCGC TCTGGCGGCC AGGCCGAGAG GGACAGAGAC 
TGGAGCCATA GCGGAAACCC CGGGGCTTCG CGGGCCGGGG AAGACGCCCG GGTTCTCAGA 
GACGGCTTTG CCGAGGAGGC CCCGAGCACG TCCCGCGGGC CGGGCGGCTC GCAGGGGTCG 
CAGGGCCCCT CGCCTCAGGG CGCCCGCCGG GCCCAGGCCG CCCCCGCCGT GGGGCCCAGG 
AGCCAGAAGC AGCTGGAGCT GAAAGTGTCC GAGCTGGTGC AGTTCTTGCT GATTAAAGAC 
CAGAAGAAGA TTCCGATCAA GCGGGCCGAC ATACTGAAGC ACGTCATCGG GGACTACAAG 
GACATCTTCC CCGACCTCTT CAAACGGGCC GCCGAGCGCC TCCAGTACGT CTTCGGGTAT 
AAGCTGGTGG AACTTGAACC CAAGAGCAAC ACTTACATCC TCATCAACAC CCTGGAGCCT 
GTGGAGGAGG ATGCCGAGAT GAGGGGTGAC CAAGGCACGC CCACTACGGG CCTCCTGATG 
ATCGTCTTAG GGCTCATCTT TATGAAGGGC AACACCATCA AGGAAACTGA AGCCTGGGAC 
TTTCTGCGGC GCTTAGGGGT CTACCCCACC AAGAAGCATT TAATTTTCGG AGATCCAAAG 
AAACTCATTA CTGAGGACTT TGTGCGACAG CGTTACCTGG AATACCGGCG GATACCCCAC 
ACCGACCCCG TCGACTACGA ATTCCAGTGG GGCCCGCGAA CCAACCTGGA AACCAGCAAG 
ATGAAAGTTC TTAAGTTTGT GGCCAAGGTC CATAATCAAG ACCCCAAGGA CTGGCCAGCG 
CAGTACTGTG AGGCTTTGGC AGATGAGGAG AACAGGGCCA GACCTCAGCC TAGTGGCCCA 
GCTCCATCCT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619649.1
CDS	118..1032
Translation	MLQKPRNRGRSGGQAERDRDWSHSGNPGASRAGEDARVLRDGFAEEAPSTSRGPGGSQGSQGPSPQGARRAQAAPAVGPRSQKQLELKVSELVQFLLIKDQKKIPIKRADILKHVIGDYKDIFPDLFKRAAERLQYVFGYKLVELEPKSNTYILINTLEPVEEDAEMRGDQGTPTTGLLMIVLGLIFMKGNTIKETEAWDFLRRLGVYPTKKHLIFGDPKKLITEDFVRQRYLEYRRIPHTDPVDYEFQWGPRTNLETSKMKVLKFVAKVHNQDPKDWPAQYCEALADEENRARPQPSGPAPSS

Target ORF information:

RefSeq Version	NM_138704.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NSE3 homolog, SMC5-SMC6 complex component (NSMCE3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138704.4

ORF Insert Sequence:

ATGTTGCAAA AACCGAGGAA CCGGGGCCGC TCTGGCGGCC AGGCCGAGAG GGACAGAGAC 
TGGAGCCATA GCGGAAACCC CGGGGCTTCG CGGGCCGGGG AAGACGCCCG GGTTCTCAGA 
GACGGCTTTG CCGAGGAGGC CCCGAGCACG TCCCGCGGGC CGGGCGGCTC GCAGGGGTCG 
CAGGGCCCCT CGCCTCAGGG CGCCCGCCGG GCCCAGGCCG CCCCCGCCGT GGGGCCCAGG 
AGCCAGAAGC AGCTGGAGCT GAAAGTGTCC GAGCTGGTGC AGTTCTTGCT GATTAAAGAC 
CAGAAGAAGA TTCCGATCAA GCGGGCCGAC ATACTGAAGC ACGTCATCGG GGACTACAAG 
GACATCTTCC CCGACCTCTT CAAACGGGCC GCCGAGCGCC TCCAGTACGT CTTCGGGTAT 
AAGCTGGTGG AACTTGAACC CAAGAGCAAC ACTTACATCC TCATCAACAC CCTGGAGCCT 
GTGGAGGAGG ATGCCGAGAT GAGGGGTGAC CAAGGCACGC CCACTACGGG CCTCCTGATG 
ATCGTCTTAG GGCTCATCTT TATGAAGGGC AACACCATCA AGGAAACTGA AGCCTGGGAC 
TTTCTGCGGC GCTTAGGGGT CTACCCCACC AAGAAGCATT TAATTTTCGG AGATCCAAAG 
AAACTCATTA CTGAGGACTT TGTGCGACAG CGTTACCTGG AATACCGGCG GATACCCCAC 
ACCGACCCCG TCGACTACGA ATTCCAGTGG GGCCCGCGAA CCAACCTGGA AACCAGCAAG 
ATGAAAGTTC TTAAGTTTGT GGCCAAGGTC CATAATCAAG ACCCCAAGGA CTGGCCAGCG 
CAGTACTGTG AGGCTTTGGC AGATGAGGAG AACAGGGCCA GACCTCAGCC TAGTGGCCCA 
GCTCCATCCT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Destabilized SMC5/6 complex leads to chromosome breakage syndrome with severe lung disease.
The Journal of clinical investigation126(8)2881-92(2016 Aug)
van der Crabben SN,Hennus MP,McGregor GA,Ritter DI,Nagamani SC,Wells OS,Harakalova M,Chinn IK,Alt A,Vondrova L,Hochstenbach R,van Montfrans JM,Terheggen-Lagro SW,van Lieshout S,van Roosmalen MJ,Renkens I,Duran K,Nijman IJ,Kloosterman WP,Hennekam E,Orange JS,van Hasselt PM,Wheeler DA,Palecek JJ,Lehmann AR,Oliver AW,Pearl LH,Plon SE,Murray JM,van Haaften G

Interactions between the Nse3 and Nse4 components of the SMC5-6 complex identify evolutionarily conserved interactions between MAGE and EID Families.
PloS one6(2)e17270(2011 Feb)
Hudson JJ,Bednarova K,Kozakova L,Liao C,Guerineau M,Colnaghi R,Vidot S,Marek J,Bathula SR,Lehmann AR,Palecek J

MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.
Molecular cell39(6)963-74(2010 Sep)
Doyle JM,Gao J,Wang J,Yang M,Potts PR

Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex.
Molecular and cellular biology28(4)1197-206(2008 Feb)
Taylor EM,Copsey AC,Hudson JJ,Vidot S,Lehmann AR

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NSMCE3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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