THSD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	THSD1
Entrez Gene ID	55901
Full Name	thrombospondin type 1 domain containing 1
Synonyms	TMTSP,UNQ3010
General protein information	Preferred Names thrombospondin type 1 domain containing 1 Names thrombospondin type-1 domain-containing protein 1 4833423O18Rik thrombospondin, type I, domain containing 1 transmembrane molecule with thrombospondin module
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q14.3
Summary	The protein encoded by this gene contains a type 1 thrombospondin domain, which is found in a number of proteins involved in the complement pathway, as well as in extracellular matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Jan 2009].
Disorder MIM:	616821

mRNA and Protein(s)

mRNA	Protein	Name
NM_199263.2	NP_954872.1	thrombospondin type-1 domain-containing protein 1 isoform 2 precursor
NM_018676.3	NP_061146.1	thrombospondin type-1 domain-containing protein 1 isoform 1 precursor
NM_199263.3	NP_954872.1	thrombospondin type-1 domain-containing protein 1 isoform 2 precursor
NM_018676.4	NP_061146.1	thrombospondin type-1 domain-containing protein 1 isoform 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	THSD1	XP_509797.4
Canis lupus familiaris (dog)	THSD1	XP_534485.2
Homo sapiens (human)	THSD1	NP_061146.1
	THSD1	XP_002800815.1
Mus musculus (house mouse)	Thsd1	NP_062522.1
Xenopus tropicalis (tropical clawed frog)	thsd1	XP_004912028.1
Rattus norvegicus (Norway rat)	Thsd1	NP_001102348.1
Gallus gallus (chicken)	THSD1	XP_417078.1
Bos taurus (cattle)	THSD1	NP_001014967.1
Danio rerio (zebrafish)	LOC797520	XP_001337990.3

Gene Ontology
Bibliography

Related articles in PubMed

The Intracranial Aneurysm Gene THSD1 Connects Endosome Dynamics to Nascent Focal Adhesion Assembly.
Rui YN, Xu Z, Fang X, Menezes MR, Balzeau J, Niu A, Hagan JP, Kim DH
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology43(6)2200-2211(2017)

THSD1 (Thrombospondin Type 1 Domain Containing Protein 1) Mutation in the Pathogenesis of Intracranial Aneurysm and Subarachnoid Hemorrhage.
Santiago-Sim T, Fang X, Hennessy ML, Nalbach SV, DePalma SR, Lee MS, Greenway SC, McDonough B, Hergenroeder GW, Patek KJ, Colosimo SM, Qualmann KJ, Hagan JP, Milewicz DM, MacRae CA, Dymecki SM, Seidman CE, Seidman JG, Kim DH
Stroke47(12)3005-3013(2016 Dec)

THSD1 preserves vascular integrity and protects against intraplaque haemorrhaging in ApoE-/- mice.
Haasdijk RA, Den Dekker WK, Cheng C, Tempel D, Szulcek R, Bos FL, Hermkens DM, Chrifi I, Brandt MM, Van Dijk C, Xu YJ, Van De Kamp EH, Blonden LA, Van Bezu J, Sluimer JC, Biessen EA, Van Nieuw Amerongen GP, Duckers HJ
Cardiovascular research110(1)129-39(2016 May)

Genome-wide screening for methylation-silenced genes in colorectal cancer.
Khamas A, Ishikawa T, Mogushi K, Iida S, Ishiguro M, Tanaka H, Uetake H, Sugihara K
International journal of oncology41(2)490-6(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

THSD1 forms a multimeric protein complex with FAK/talin/vinculin, wherein THSD1 promotes talin binding to FAK but not to vinculin, a key step in nascent adhesion assembly.
Title: The Intracranial Aneurysm Gene THSD1 Connects Endosome Dynamics to Nascent Focal Adhesion Assembly.

THSD1 mutation associated with Intracranial Aneurysm and Subarachnoid Hemorrhage.
Title: THSD1 (Thrombospondin Type 1 Domain Containing Protein 1) Mutation in the Pathogenesis of Intracranial Aneurysm and Subarachnoid Hemorrhage.

thrombospondin type I domain 1 is a new regulator of endothelial barrier function during vascular development and protects intraplaque microvessels against haemorrhaging in advanced atherosclerotic lesions
Title: THSD1 preserves vascular integrity and protects against intraplaque haemorrhaging in ApoE-/- mice.

The THSD1 gene may play a role in the tumorigenesis of colorectal cancer
Title: Genome-wide screening for methylation-silenced genes in colorectal cancer.

These findings suggest that THSD1 is a good candidate tumor suppressor gene.
Title: Monochromosome transfer and microarray analysis identify a critical tumor-suppressive region mapping to chromosome 13q14 and THSD1 in esophageal carcinoma.

The following THSD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THSD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02790	NM_199263.3 Latest version!	Homo sapiens thrombospondin type 1 domain containing 1 (THSD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu08667	NM_018676.4 Latest version!	Homo sapiens thrombospondin type 1 domain containing 1 (THSD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
View Old Accession Versions >>
OHu02790	NM_199263.2	Homo sapiens thrombospondin type 1 domain containing 1 (THSD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu08667	NM_018676.3	Homo sapiens thrombospondin type 1 domain containing 1 (THSD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THSD1 ( NM_199263.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_954872.1 Homo sapiens thrombospondin type 1 domain containing 1 (THSD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02790
Clone ID Related Accession (Same CDS sequence)	NM_199263.2 , NM_199263.3
Accession Version	NM_199263.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	thrombospondin type-1 domain-containing protein 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096289.1 and BC063842.1. On Jan 24, 2009 this sequence version replaced NM_199263.1. Transcript Variant: This variant (2) is missing an in-frame coding exon compared to transcript variant 1, resulting in a shorter isoform (2) lacking an internal segment compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK096289.1, SRR1660803.98658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267763 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACCAA TGTTGAAAGA CTTTTCAAAT CTATTGTTGG TGGTACTCTG TGACTATGTT 
CTTGGAGAAG CTGAATATCT TCTCTTGAGA GAGCCAGGCC ATGTAGCACT AAGCAACGAC 
ACAGTGTATG TGGATTTCCA GTATTTTGAT GGTGCTAATG GGACACTGAG GAATGTATCT 
GTCCTGCTGT TGGAGGCCAA CACCAATCAG ACTGTAACTA CCAAGTACCT CCTGACCAAC 
CAGTCCCAGG GAACACTAAA GTTTGAGTGC TTCTATTTCA AGGAGGCTGG TGACTACTGG 
TTCACAATGA CTCCAGAAGC AACAGACAAC AGCACTCCAT TCCCCTGGTG GGAGAAAAGT 
GCCTTTCTGA AGGTGGAATG GCCTGTCTTT CACGTTGACT TGAATAGGAG TGCCAAGGCA 
GCAGAAGGCA CCTTCCAAGT GGGCCTATTT ACCAGTCAAC CACTGTGCCC GTTTCCTGTG 
GACAAGCCCA ACATCGTAGT GGATGTCATC TTCACCAACA GTCTTCCTGA GGCAAGAAGA 
AATTCAAGAC AGCCGCTGGA AATAAGAACC AGCAAAAGGA CAGAACTTGC TCAAGGTCAG 
TGGGTTGAGT TTGGCTGTGC ACCCTTGGGG CCAGAAGCCT ATGTCACCGT GGTGCTGAAG 
CTGCTTGGGC GAGACTCAGT CATTACCTCC ACAGGACCCA TTGACCTGGC CCAGAAATTT 
GGATACAAAC TGGTGATGGT GCCAGAACTC ACATGTGAGT CCGGGGTAGA GGTGACAGTG 
CTGCCTCCAC CATGCACCTT CGTCCAAGGA GTGGTCACTG TCTTCAAGGA GGCCCCCAGA 
TACCCTGGGA AGAGGACCAT TCACTTGGCT GAAAACAGCC TGCCCCTGGG AGAGAGGAGG 
ACAATTTTTA ACTGTACTTT GTTTGACATG GGGAAGAATA AGTACTGCTT TGACTTTGGC 
ATTTCAAGCA GAAGCCATTT TTCTGCAAAG GAGGAGTGCA TGCTAATTCA GAGAAATACA 
GCTTTCCAGC CATCCAGCCC ATCTCCTCTT CAGCCCCAGG GTCCAGTGAA GTCCAACAAC 
ATCGTGACTG TCACTGGTAT ATCCTTGTGC TTGTTCATCA TCATTGCCAC TGTGCTCATC 
ACGCTGTGGA GGAGGTTCGG CCGGCCAGCC AAGTGCAGCA CACCTGCTCG ACACAACTCC 
ATCCACTCCC CCAGCTTCCG GAAGAACTCG GACGAGGAGA ATATCTGCGA GCTGAGCGAG 
CAGCGCGGGA GCTTCTCGGA TGGGGGAGAC GGGCCCACGG GGAGTCCAGG GGACACAGGC 
ATCCCTCTGA CCTACAGGCG GAGCGGGCCG GTACCTCCCG AGGATGATGC CTCTGGCAGC 
GAGAGCTTCC AGTCCAACGC CCAGAAGATA ATCCCACCTC TGTTCAGCTA CCGCCTTGCC 
CAGCAGCAGT TAAAGGAGAT GAAAAAGAAA GGTCTGACGG AAACTACCAA AGTGTATCAC 
GTGTCTCAGA GTCCCCTGAC AGACACTGCC ATTGATGCGG CCCCCAGCGC TCCCTTAGAT 
TTGGAAAGCC CGGAAGAAGC TGCAGCAAAC AAGTTCCGGA TCAAATCCCC ATTTCCGGAG 
CAGCCCGCGG TCAGTGCCGG GGAAAGGCCT CCCTCCAGGC TGGATCTAAA TGTGACTCAG 
GCCAGTTGTG CCATAAGCCC CAGCCAGACT CTGATCCGCA AGTCACAGGC AAGGCACGTG 
GGCAGCAGAG GGGGCCCGTC CGAAAGGAGC CATGCCAGGA ACGCCCATTT CAGGAGGACA 
GCGAGTTTCC ATGAAGCCAG GCAGGCCCGG CCGTTCCGAG AGAGGAGCAT GTCCACTCTG 
ACTCCACGGC AGGCCCCTGC CTACAGCTCT AGGACGCGGA CCTGCGAGCA GGCAGAGGAC 
AGATTTAGGC CTCAGAGTCG AGGTGCCCAC CTGTTTCCTG AAAAACTGGA GCATTTCCAA 
GAGGCAAGTG GAACCCGTGG TCCATTAAAC CCTCTCCCTA AATCCTACAC TTTGGGGCAG 
CCCTTGAGGA AACCAGACCT TGGGGATCAC CAGGCAGGAT TAGTGGCCGG AATTGAGAGA 
ACAGAGCCCC ACAGAGCTCG TCGGGGACCG TCCCCCAGTC ACAAGAGTGT CTCAAGGAAG 
CAGTCTTCTC CCATATCCCC CAAAGATAAC TACCAGAGGG TCAGTTCTCT GAGCCCTTCT 
CAGTGTAGAA AAGACAAGTG TCAAAGCTTC CCCACTCACC CTGAGTTTGC CTTCTATGAC 
AATACGTCGT TTGGCCTCAC TGAGGCTGAG CAGAGGATGC TGGACCTCCC AGGATATTTT 
GGGTCAAATG AAGAGGATGA AACCACAAGT ACACTTAGCG TGGAGAAGCT GGTGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954872.1
CDS	546..2945
Translation	MKPMLKDFSNLLLVVLCDYVLGEAEYLLLREPGHVALSNDTVYVDFQYFDGANGTLRNVSVLLLEANTNQTVTTKYLLTNQSQGTLKFECFYFKEAGDYWFTMTPEATDNSTPFPWWEKSAFLKVEWPVFHVDLNRSAKAAEGTFQVGLFTSQPLCPFPVDKPNIVVDVIFTNSLPEARRNSRQPLEIRTSKRTELAQGQWVEFGCAPLGPEAYVTVVLKLLGRDSVITSTGPIDLAQKFGYKLVMVPELTCESGVEVTVLPPPCTFVQGVVTVFKEAPRYPGKRTIHLAENSLPLGERRTIFNCTLFDMGKNKYCFDFGISSRSHFSAKEECMLIQRNTAFQPSSPSPLQPQGPVKSNNIVTVTGISLCLFIIIATVLITLWRRFGRPAKCSTPARHNSIHSPSFRKNSDEENICELSEQRGSFSDGGDGPTGSPGDTGIPLTYRRSGPVPPEDDASGSESFQSNAQKIIPPLFSYRLAQQQLKEMKKKGLTETTKVYHVSQSPLTDTAIDAAPSAPLDLESPEEAAANKFRIKSPFPEQPAVSAGERPPSRLDLNVTQASCAISPSQTLIRKSQARHVGSRGGPSERSHARNAHFRRTASFHEARQARPFRERSMSTLTPRQAPAYSSRTRTCEQAEDRFRPQSRGAHLFPEKLEHFQEASGTRGPLNPLPKSYTLGQPLRKPDLGDHQAGLVAGIERTEPHRARRGPSPSHKSVSRKQSSPISPKDNYQRVSSLSPSQCRKDKCQSFPTHPEFAFYDNTSFGLTEAEQRMLDLPGYFGSNEEDETTSTLSVEKLVI

Target ORF information:

RefSeq Version	NM_199263.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type 1 domain containing 1 (THSD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199263.2

ORF Insert Sequence:

ATGAAACCAA TGTTGAAAGA CTTTTCAAAT CTATTGTTGG TGGTACTCTG TGACTATGTT 
CTTGGAGAAG CTGAATATCT TCTCTTGAGA GAGCCAGGCC ATGTAGCACT AAGCAACGAC 
ACAGTGTATG TGGATTTCCA GTATTTTGAT GGTGCTAATG GGACACTGAG GAATGTATCT 
GTCCTGCTGT TGGAGGCCAA CACCAATCAG ACTGTAACTA CCAAGTACCT CCTGACCAAC 
CAGTCCCAGG GAACACTAAA GTTTGAGTGC TTCTATTTCA AGGAGGCTGG TGACTACTGG 
TTCACAATGA CTCCAGAAGC AACAGACAAC AGCACTCCAT TCCCCTGGTG GGAGAAAAGT 
GCCTTTCTGA AGGTGGAATG GCCTGTCTTT CACGTTGACT TGAATAGGAG TGCCAAGGCA 
GCAGAAGGCA CCTTCCAAGT GGGCCTATTT ACCAGTCAAC CACTGTGCCC GTTTCCTGTG 
GACAAGCCCA ACATCGTAGT GGATGTCATC TTCACCAACA GTCTTCCTGA GGCAAGAAGA 
AATTCAAGAC AGCCGCTGGA AATAAGAACC AGCAAAAGGA CAGAACTTGC TCAAGGTCAG 
TGGGTTGAGT TTGGCTGTGC ACCCTTGGGG CCAGAAGCCT ATGTCACCGT GGTGCTGAAG 
CTGCTTGGGC GAGACTCAGT CATTACCTCC ACAGGACCCA TTGACCTGGC CCAGAAATTT 
GGATACAAAC TGGTGATGGT GCCAGAACTC ACATGTGAGT CCGGGGTAGA GGTGACAGTG 
CTGCCTCCAC CATGCACCTT CGTCCAAGGA GTGGTCACTG TCTTCAAGGA GGCCCCCAGA 
TACCCTGGGA AGAGGACCAT TCACTTGGCT GAAAACAGCC TGCCCCTGGG AGAGAGGAGG 
ACAATTTTTA ACTGTACTTT GTTTGACATG GGGAAGAATA AGTACTGCTT TGACTTTGGC 
ATTTCAAGCA GAAGCCATTT TTCTGCAAAG GAGGAGTGCA TGCTAATTCA GAGAAATACA 
GCTTTCCAGC CATCCAGCCC ATCTCCTCTT CAGCCCCAGG GTCCAGTGAA GTCCAACAAC 
ATCGTGACTG TCACTGGTAT ATCCTTGTGC TTGTTCATCA TCATTGCCAC TGTGCTCATC 
ACGCTGTGGA GGAGGTTCGG CCGGCCAGCC AAGTGCAGCA CACCTGCTCG ACACAACTCC 
ATCCACTCCC CCAGCTTCCG GAAGAACTCG GACGAGGAGA ATATCTGCGA GCTGAGCGAG 
CAGCGCGGGA GCTTCTCGGA TGGGGGAGAC GGGCCCACGG GGAGTCCAGG GGACACAGGC 
ATCCCTCTGA CCTACAGGCG GAGCGGGCCG GTACCTCCCG AGGATGATGC CTCTGGCAGC 
GAGAGCTTCC AGTCCAACGC CCAGAAGATA ATCCCACCTC TGTTCAGCTA CCGCCTTGCC 
CAGCAGCAGT TAAAGGAGAT GAAAAAGAAA GGTCTGACGG AAACTACCAA AGTGTATCAC 
GTGTCTCAGA GTCCCCTGAC AGACACTGCC ATTGATGCGG CCCCCAGCGC TCCCTTAGAT 
TTGGAAAGCC CGGAAGAAGC TGCAGCAAAC AAGTTCCGGA TCAAATCCCC ATTTCCGGAG 
CAGCCCGCGG TCAGTGCCGG GGAAAGGCCT CCCTCCAGGC TGGATCTAAA TGTGACTCAG 
GCCAGTTGTG CCATAAGCCC CAGCCAGACT CTGATCCGCA AGTCACAGGC AAGGCACGTG 
GGCAGCAGAG GGGGCCCGTC CGAAAGGAGC CATGCCAGGA ACGCCCATTT CAGGAGGACA 
GCGAGTTTCC ATGAAGCCAG GCAGGCCCGG CCGTTCCGAG AGAGGAGCAT GTCCACTCTG 
ACTCCACGGC AGGCCCCTGC CTACAGCTCT AGGACGCGGA CCTGCGAGCA GGCAGAGGAC 
AGATTTAGGC CTCAGAGTCG AGGTGCCCAC CTGTTTCCTG AAAAACTGGA GCATTTCCAA 
GAGGCAAGTG GAACCCGTGG TCCATTAAAC CCTCTCCCTA AATCCTACAC TTTGGGGCAG 
CCCTTGAGGA AACCAGACCT TGGGGATCAC CAGGCAGGAT TAGTGGCCGG AATTGAGAGA 
ACAGAGCCCC ACAGAGCTCG TCGGGGACCG TCCCCCAGTC ACAAGAGTGT CTCAAGGAAG 
CAGTCTTCTC CCATATCCCC CAAAGATAAC TACCAGAGGG TCAGTTCTCT GAGCCCTTCT 
CAGTGTAGAA AAGACAAGTG TCAAAGCTTC CCCACTCACC CTGAGTTTGC CTTCTATGAC 
AATACGTCGT TTGGCCTCAC TGAGGCTGAG CAGAGGATGC TGGACCTCCC AGGATATTTT 
GGGTCAAATG AAGAGGATGA AACCACAAGT ACACTTAGCG TGGAGAAGCT GGTGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THSD1 ( NM_199263.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_954872.1 Homo sapiens thrombospondin type 1 domain containing 1 (THSD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02790
Clone ID Related Accession (Same CDS sequence)	NM_199263.2 , NM_199263.3
Accession Version	NM_199263.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Homo sapiens(human)
Product	thrombospondin type-1 domain-containing protein 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096289.1 and BC063842.1. On May 31, 2019 this sequence version replaced NM_199263.2. Transcript Variant: This variant (2) is missing an in-frame coding exon compared to transcript variant 1, resulting in a shorter isoform (2) lacking an internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR6380200.153594.19, AK096289.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267763 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACCAA TGTTGAAAGA CTTTTCAAAT CTATTGTTGG TGGTACTCTG TGACTATGTT 
CTTGGAGAAG CTGAATATCT TCTCTTGAGA GAGCCAGGCC ATGTAGCACT AAGCAACGAC 
ACAGTGTATG TGGATTTCCA GTATTTTGAT GGTGCTAATG GGACACTGAG GAATGTATCT 
GTCCTGCTGT TGGAGGCCAA CACCAATCAG ACTGTAACTA CCAAGTACCT CCTGACCAAC 
CAGTCCCAGG GAACACTAAA GTTTGAGTGC TTCTATTTCA AGGAGGCTGG TGACTACTGG 
TTCACAATGA CTCCAGAAGC AACAGACAAC AGCACTCCAT TCCCCTGGTG GGAGAAAAGT 
GCCTTTCTGA AGGTGGAATG GCCTGTCTTT CACGTTGACT TGAATAGGAG TGCCAAGGCA 
GCAGAAGGCA CCTTCCAAGT GGGCCTATTT ACCAGTCAAC CACTGTGCCC GTTTCCTGTG 
GACAAGCCCA ACATCGTAGT GGATGTCATC TTCACCAACA GTCTTCCTGA GGCAAGAAGA 
AATTCAAGAC AGCCGCTGGA AATAAGAACC AGCAAAAGGA CAGAACTTGC TCAAGGTCAG 
TGGGTTGAGT TTGGCTGTGC ACCCTTGGGG CCAGAAGCCT ATGTCACCGT GGTGCTGAAG 
CTGCTTGGGC GAGACTCAGT CATTACCTCC ACAGGACCCA TTGACCTGGC CCAGAAATTT 
GGATACAAAC TGGTGATGGT GCCAGAACTC ACATGTGAGT CCGGGGTAGA GGTGACAGTG 
CTGCCTCCAC CATGCACCTT CGTCCAAGGA GTGGTCACTG TCTTCAAGGA GGCCCCCAGA 
TACCCTGGGA AGAGGACCAT TCACTTGGCT GAAAACAGCC TGCCCCTGGG AGAGAGGAGG 
ACAATTTTTA ACTGTACTTT GTTTGACATG GGGAAGAATA AGTACTGCTT TGACTTTGGC 
ATTTCAAGCA GAAGCCATTT TTCTGCAAAG GAGGAGTGCA TGCTAATTCA GAGAAATACA 
GCTTTCCAGC CATCCAGCCC ATCTCCTCTT CAGCCCCAGG GTCCAGTGAA GTCCAACAAC 
ATCGTGACTG TCACTGGTAT ATCCTTGTGC TTGTTCATCA TCATTGCCAC TGTGCTCATC 
ACGCTGTGGA GGAGGTTCGG CCGGCCAGCC AAGTGCAGCA CACCTGCTCG ACACAACTCC 
ATCCACTCCC CCAGCTTCCG GAAGAACTCG GACGAGGAGA ATATCTGCGA GCTGAGCGAG 
CAGCGCGGGA GCTTCTCGGA TGGGGGAGAC GGGCCCACGG GGAGTCCAGG GGACACAGGC 
ATCCCTCTGA CCTACAGGCG GAGCGGGCCG GTACCTCCCG AGGATGATGC CTCTGGCAGC 
GAGAGCTTCC AGTCCAACGC CCAGAAGATA ATCCCACCTC TGTTCAGCTA CCGCCTTGCC 
CAGCAGCAGT TAAAGGAGAT GAAAAAGAAA GGTCTGACGG AAACTACCAA AGTGTATCAC 
GTGTCTCAGA GTCCCCTGAC AGACACTGCC ATTGATGCGG CCCCCAGCGC TCCCTTAGAT 
TTGGAAAGCC CGGAAGAAGC TGCAGCAAAC AAGTTCCGGA TCAAATCCCC ATTTCCGGAG 
CAGCCCGCGG TCAGTGCCGG GGAAAGGCCT CCCTCCAGGC TGGATCTAAA TGTGACTCAG 
GCCAGTTGTG CCATAAGCCC CAGCCAGACT CTGATCCGCA AGTCACAGGC AAGGCACGTG 
GGCAGCAGAG GGGGCCCGTC CGAAAGGAGC CATGCCAGGA ACGCCCATTT CAGGAGGACA 
GCGAGTTTCC ATGAAGCCAG GCAGGCCCGG CCGTTCCGAG AGAGGAGCAT GTCCACTCTG 
ACTCCACGGC AGGCCCCTGC CTACAGCTCT AGGACGCGGA CCTGCGAGCA GGCAGAGGAC 
AGATTTAGGC CTCAGAGTCG AGGTGCCCAC CTGTTTCCTG AAAAACTGGA GCATTTCCAA 
GAGGCAAGTG GAACCCGTGG TCCATTAAAC CCTCTCCCTA AATCCTACAC TTTGGGGCAG 
CCCTTGAGGA AACCAGACCT TGGGGATCAC CAGGCAGGAT TAGTGGCCGG AATTGAGAGA 
ACAGAGCCCC ACAGAGCTCG TCGGGGACCG TCCCCCAGTC ACAAGAGTGT CTCAAGGAAG 
CAGTCTTCTC CCATATCCCC CAAAGATAAC TACCAGAGGG TCAGTTCTCT GAGCCCTTCT 
CAGTGTAGAA AAGACAAGTG TCAAAGCTTC CCCACTCACC CTGAGTTTGC CTTCTATGAC 
AATACGTCGT TTGGCCTCAC TGAGGCTGAG CAGAGGATGC TGGACCTCCC AGGATATTTT 
GGGTCAAATG AAGAGGATGA AACCACAAGT ACACTTAGCG TGGAGAAGCT GGTGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954872.1
CDS	224..2623
Translation	MKPMLKDFSNLLLVVLCDYVLGEAEYLLLREPGHVALSNDTVYVDFQYFDGANGTLRNVSVLLLEANTNQTVTTKYLLTNQSQGTLKFECFYFKEAGDYWFTMTPEATDNSTPFPWWEKSAFLKVEWPVFHVDLNRSAKAAEGTFQVGLFTSQPLCPFPVDKPNIVVDVIFTNSLPEARRNSRQPLEIRTSKRTELAQGQWVEFGCAPLGPEAYVTVVLKLLGRDSVITSTGPIDLAQKFGYKLVMVPELTCESGVEVTVLPPPCTFVQGVVTVFKEAPRYPGKRTIHLAENSLPLGERRTIFNCTLFDMGKNKYCFDFGISSRSHFSAKEECMLIQRNTAFQPSSPSPLQPQGPVKSNNIVTVTGISLCLFIIIATVLITLWRRFGRPAKCSTPARHNSIHSPSFRKNSDEENICELSEQRGSFSDGGDGPTGSPGDTGIPLTYRRSGPVPPEDDASGSESFQSNAQKIIPPLFSYRLAQQQLKEMKKKGLTETTKVYHVSQSPLTDTAIDAAPSAPLDLESPEEAAANKFRIKSPFPEQPAVSAGERPPSRLDLNVTQASCAISPSQTLIRKSQARHVGSRGGPSERSHARNAHFRRTASFHEARQARPFRERSMSTLTPRQAPAYSSRTRTCEQAEDRFRPQSRGAHLFPEKLEHFQEASGTRGPLNPLPKSYTLGQPLRKPDLGDHQAGLVAGIERTEPHRARRGPSPSHKSVSRKQSSPISPKDNYQRVSSLSPSQCRKDKCQSFPTHPEFAFYDNTSFGLTEAEQRMLDLPGYFGSNEEDETTSTLSVEKLVI

Target ORF information:

RefSeq Version	NM_199263.3
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type 1 domain containing 1 (THSD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199263.3

ORF Insert Sequence:

ATGAAACCAA TGTTGAAAGA CTTTTCAAAT CTATTGTTGG TGGTACTCTG TGACTATGTT 
CTTGGAGAAG CTGAATATCT TCTCTTGAGA GAGCCAGGCC ATGTAGCACT AAGCAACGAC 
ACAGTGTATG TGGATTTCCA GTATTTTGAT GGTGCTAATG GGACACTGAG GAATGTATCT 
GTCCTGCTGT TGGAGGCCAA CACCAATCAG ACTGTAACTA CCAAGTACCT CCTGACCAAC 
CAGTCCCAGG GAACACTAAA GTTTGAGTGC TTCTATTTCA AGGAGGCTGG TGACTACTGG 
TTCACAATGA CTCCAGAAGC AACAGACAAC AGCACTCCAT TCCCCTGGTG GGAGAAAAGT 
GCCTTTCTGA AGGTGGAATG GCCTGTCTTT CACGTTGACT TGAATAGGAG TGCCAAGGCA 
GCAGAAGGCA CCTTCCAAGT GGGCCTATTT ACCAGTCAAC CACTGTGCCC GTTTCCTGTG 
GACAAGCCCA ACATCGTAGT GGATGTCATC TTCACCAACA GTCTTCCTGA GGCAAGAAGA 
AATTCAAGAC AGCCGCTGGA AATAAGAACC AGCAAAAGGA CAGAACTTGC TCAAGGTCAG 
TGGGTTGAGT TTGGCTGTGC ACCCTTGGGG CCAGAAGCCT ATGTCACCGT GGTGCTGAAG 
CTGCTTGGGC GAGACTCAGT CATTACCTCC ACAGGACCCA TTGACCTGGC CCAGAAATTT 
GGATACAAAC TGGTGATGGT GCCAGAACTC ACATGTGAGT CCGGGGTAGA GGTGACAGTG 
CTGCCTCCAC CATGCACCTT CGTCCAAGGA GTGGTCACTG TCTTCAAGGA GGCCCCCAGA 
TACCCTGGGA AGAGGACCAT TCACTTGGCT GAAAACAGCC TGCCCCTGGG AGAGAGGAGG 
ACAATTTTTA ACTGTACTTT GTTTGACATG GGGAAGAATA AGTACTGCTT TGACTTTGGC 
ATTTCAAGCA GAAGCCATTT TTCTGCAAAG GAGGAGTGCA TGCTAATTCA GAGAAATACA 
GCTTTCCAGC CATCCAGCCC ATCTCCTCTT CAGCCCCAGG GTCCAGTGAA GTCCAACAAC 
ATCGTGACTG TCACTGGTAT ATCCTTGTGC TTGTTCATCA TCATTGCCAC TGTGCTCATC 
ACGCTGTGGA GGAGGTTCGG CCGGCCAGCC AAGTGCAGCA CACCTGCTCG ACACAACTCC 
ATCCACTCCC CCAGCTTCCG GAAGAACTCG GACGAGGAGA ATATCTGCGA GCTGAGCGAG 
CAGCGCGGGA GCTTCTCGGA TGGGGGAGAC GGGCCCACGG GGAGTCCAGG GGACACAGGC 
ATCCCTCTGA CCTACAGGCG GAGCGGGCCG GTACCTCCCG AGGATGATGC CTCTGGCAGC 
GAGAGCTTCC AGTCCAACGC CCAGAAGATA ATCCCACCTC TGTTCAGCTA CCGCCTTGCC 
CAGCAGCAGT TAAAGGAGAT GAAAAAGAAA GGTCTGACGG AAACTACCAA AGTGTATCAC 
GTGTCTCAGA GTCCCCTGAC AGACACTGCC ATTGATGCGG CCCCCAGCGC TCCCTTAGAT 
TTGGAAAGCC CGGAAGAAGC TGCAGCAAAC AAGTTCCGGA TCAAATCCCC ATTTCCGGAG 
CAGCCCGCGG TCAGTGCCGG GGAAAGGCCT CCCTCCAGGC TGGATCTAAA TGTGACTCAG 
GCCAGTTGTG CCATAAGCCC CAGCCAGACT CTGATCCGCA AGTCACAGGC AAGGCACGTG 
GGCAGCAGAG GGGGCCCGTC CGAAAGGAGC CATGCCAGGA ACGCCCATTT CAGGAGGACA 
GCGAGTTTCC ATGAAGCCAG GCAGGCCCGG CCGTTCCGAG AGAGGAGCAT GTCCACTCTG 
ACTCCACGGC AGGCCCCTGC CTACAGCTCT AGGACGCGGA CCTGCGAGCA GGCAGAGGAC 
AGATTTAGGC CTCAGAGTCG AGGTGCCCAC CTGTTTCCTG AAAAACTGGA GCATTTCCAA 
GAGGCAAGTG GAACCCGTGG TCCATTAAAC CCTCTCCCTA AATCCTACAC TTTGGGGCAG 
CCCTTGAGGA AACCAGACCT TGGGGATCAC CAGGCAGGAT TAGTGGCCGG AATTGAGAGA 
ACAGAGCCCC ACAGAGCTCG TCGGGGACCG TCCCCCAGTC ACAAGAGTGT CTCAAGGAAG 
CAGTCTTCTC CCATATCCCC CAAAGATAAC TACCAGAGGG TCAGTTCTCT GAGCCCTTCT 
CAGTGTAGAA AAGACAAGTG TCAAAGCTTC CCCACTCACC CTGAGTTTGC CTTCTATGAC 
AATACGTCGT TTGGCCTCAC TGAGGCTGAG CAGAGGATGC TGGACCTCCC AGGATATTTT 
GGGTCAAATG AAGAGGATGA AACCACAAGT ACACTTAGCG TGGAGAAGCT GGTGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THSD1 ( NM_018676.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_061146.1 Homo sapiens thrombospondin type 1 domain containing 1 (THSD1), transcript variant 1, mRNA.

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CloneID	OHu08667
Clone ID Related Accession (Same CDS sequence)	NM_018676.3 , NM_018676.4
Accession Version	NM_018676.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	thrombospondin type-1 domain-containing protein 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096289.1, AL359513.12 and BC063842.1. This sequence is a reference standard in the RefSeqGene project. On Jan 24, 2009 this sequence version replaced NM_018676.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803613.226789.1, SRR1803612.241247.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACCAA TGTTGAAAGA CTTTTCAAAT CTATTGTTGG TGGTACTCTG TGACTATGTT 
CTTGGAGAAG CTGAATATCT TCTCTTGAGA GAGCCAGGCC ATGTAGCACT AAGCAACGAC 
ACAGTGTATG TGGATTTCCA GTATTTTGAT GGTGCTAATG GGACACTGAG GAATGTATCT 
GTCCTGCTGT TGGAGGCCAA CACCAATCAG ACTGTAACTA CCAAGTACCT CCTGACCAAC 
CAGTCCCAGG GAACACTAAA GTTTGAGTGC TTCTATTTCA AGGAGGCTGG TGACTACTGG 
TTCACAATGA CTCCAGAAGC AACAGACAAC AGCACTCCAT TCCCCTGGTG GGAGAAAAGT 
GCCTTTCTGA AGGTGGAATG GCCTGTCTTT CACGTTGACT TGAATAGGAG TGCCAAGGCA 
GCAGAAGGCA CCTTCCAAGT GGGCCTATTT ACCAGTCAAC CACTGTGCCC GTTTCCTGTG 
GACAAGCCCA ACATCGTAGT GGATGTCATC TTCACCAACA GTCTTCCTGA GGCAAGAAGA 
AATTCAAGAC AGCCGCTGGA AATAAGAACC AGCAAAAGGA CAGAACTTGC TCAAGGTCAG 
TGGGTTGAGT TTGGCTGTGC ACCCTTGGGG CCAGAAGCCT ATGTCACCGT GGTGCTGAAG 
CTGCTTGGGC GAGACTCAGT CATTACCTCC ACAGGACCCA TTGACCTGGC CCAGAAATTT 
GGATACAAAC TGGTGATGGT GCCAGAACTC ACATGTGAGT CCGGGGTAGA GGTGACAGTG 
CTGCCTCCAC CATGCACCTT CGTCCAAGGA GTGGTCACTG TCTTCAAGGA GGCCCCCAGA 
TACCCTGGGA AGAGGACCAT TCACTTGGCT GAAAACAGCC TGCCCCTGGG AGAGAGGAGG 
ACAATTTTTA ACTGTACTTT GTTTGACATG GGGAAGAATA AGTACTGCTT TGACTTTGGC 
ATTTCAAGCA GAAGCCATTT TTCTGCAAAG GAGGAGTGCA TGCTAATTCA GAGAAATACA 
GAAACTTGGG GACTGTGGCA GCCATGGAGC CAGTGTAGTG CCACATGTGG GGATGGTGTC 
AGAGAGCGTC GCCGAGTGTG TCTCACTTCC TTCCCCTCCA GTCCTGTCTG CCCTGGAATG 
TCCTTGGAGG CCTCCCTGTG TTCCCTGGAG GAGTGTGCTG CTTTCCAGCC ATCCAGCCCA 
TCTCCTCTTC AGCCCCAGGG TCCAGTGAAG TCCAACAACA TCGTGACTGT CACTGGTATA 
TCCTTGTGCT TGTTCATCAT CATTGCCACT GTGCTCATCA CGCTGTGGAG GAGGTTCGGC 
CGGCCAGCCA AGTGCAGCAC ACCTGCTCGA CACAACTCCA TCCACTCCCC CAGCTTCCGG 
AAGAACTCGG ACGAGGAGAA TATCTGCGAG CTGAGCGAGC AGCGCGGGAG CTTCTCGGAT 
GGGGGAGACG GGCCCACGGG GAGTCCAGGG GACACAGGCA TCCCTCTGAC CTACAGGCGG 
AGCGGGCCGG TACCTCCCGA GGATGATGCC TCTGGCAGCG AGAGCTTCCA GTCCAACGCC 
CAGAAGATAA TCCCACCTCT GTTCAGCTAC CGCCTTGCCC AGCAGCAGTT AAAGGAGATG 
AAAAAGAAAG GTCTGACGGA AACTACCAAA GTGTATCACG TGTCTCAGAG TCCCCTGACA 
GACACTGCCA TTGATGCGGC CCCCAGCGCT CCCTTAGATT TGGAAAGCCC GGAAGAAGCT 
GCAGCAAACA AGTTCCGGAT CAAATCCCCA TTTCCGGAGC AGCCCGCGGT CAGTGCCGGG 
GAAAGGCCTC CCTCCAGGCT GGATCTAAAT GTGACTCAGG CCAGTTGTGC CATAAGCCCC 
AGCCAGACTC TGATCCGCAA GTCACAGGCA AGGCACGTGG GCAGCAGAGG GGGCCCGTCC 
GAAAGGAGCC ATGCCAGGAA CGCCCATTTC AGGAGGACAG CGAGTTTCCA TGAAGCCAGG 
CAGGCCCGGC CGTTCCGAGA GAGGAGCATG TCCACTCTGA CTCCACGGCA GGCCCCTGCC 
TACAGCTCTA GGACGCGGAC CTGCGAGCAG GCAGAGGACA GATTTAGGCC TCAGAGTCGA 
GGTGCCCACC TGTTTCCTGA AAAACTGGAG CATTTCCAAG AGGCAAGTGG AACCCGTGGT 
CCATTAAACC CTCTCCCTAA ATCCTACACT TTGGGGCAGC CCTTGAGGAA ACCAGACCTT 
GGGGATCACC AGGCAGGATT AGTGGCCGGA ATTGAGAGAA CAGAGCCCCA CAGAGCTCGT 
CGGGGACCGT CCCCCAGTCA CAAGAGTGTC TCAAGGAAGC AGTCTTCTCC CATATCCCCC 
AAAGATAACT ACCAGAGGGT CAGTTCTCTG AGCCCTTCTC AGTGTAGAAA AGACAAGTGT 
CAAAGCTTCC CCACTCACCC TGAGTTTGCC TTCTATGACA ATACGTCGTT TGGCCTCACT 
GAGGCTGAGC AGAGGATGCT GGACCTCCCA GGATATTTTG GGTCAAATGA AGAGGATGAA 
ACCACAAGTA CACTTAGCGT GGAGAAGCTG GTGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061146.1
CDS	546..3104
Translation	MKPMLKDFSNLLLVVLCDYVLGEAEYLLLREPGHVALSNDTVYVDFQYFDGANGTLRNVSVLLLEANTNQTVTTKYLLTNQSQGTLKFECFYFKEAGDYWFTMTPEATDNSTPFPWWEKSAFLKVEWPVFHVDLNRSAKAAEGTFQVGLFTSQPLCPFPVDKPNIVVDVIFTNSLPEARRNSRQPLEIRTSKRTELAQGQWVEFGCAPLGPEAYVTVVLKLLGRDSVITSTGPIDLAQKFGYKLVMVPELTCESGVEVTVLPPPCTFVQGVVTVFKEAPRYPGKRTIHLAENSLPLGERRTIFNCTLFDMGKNKYCFDFGISSRSHFSAKEECMLIQRNTETWGLWQPWSQCSATCGDGVRERRRVCLTSFPSSPVCPGMSLEASLCSLEECAAFQPSSPSPLQPQGPVKSNNIVTVTGISLCLFIIIATVLITLWRRFGRPAKCSTPARHNSIHSPSFRKNSDEENICELSEQRGSFSDGGDGPTGSPGDTGIPLTYRRSGPVPPEDDASGSESFQSNAQKIIPPLFSYRLAQQQLKEMKKKGLTETTKVYHVSQSPLTDTAIDAAPSAPLDLESPEEAAANKFRIKSPFPEQPAVSAGERPPSRLDLNVTQASCAISPSQTLIRKSQARHVGSRGGPSERSHARNAHFRRTASFHEARQARPFRERSMSTLTPRQAPAYSSRTRTCEQAEDRFRPQSRGAHLFPEKLEHFQEASGTRGPLNPLPKSYTLGQPLRKPDLGDHQAGLVAGIERTEPHRARRGPSPSHKSVSRKQSSPISPKDNYQRVSSLSPSQCRKDKCQSFPTHPEFAFYDNTSFGLTEAEQRMLDLPGYFGSNEEDETTSTLSVEKLVI

Target ORF information:

RefSeq Version	NM_018676.3
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type 1 domain containing 1 (THSD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018676.3

ORF Insert Sequence:

ATGAAACCAA TGTTGAAAGA CTTTTCAAAT CTATTGTTGG TGGTACTCTG TGACTATGTT 
CTTGGAGAAG CTGAATATCT TCTCTTGAGA GAGCCAGGCC ATGTAGCACT AAGCAACGAC 
ACAGTGTATG TGGATTTCCA GTATTTTGAT GGTGCTAATG GGACACTGAG GAATGTATCT 
GTCCTGCTGT TGGAGGCCAA CACCAATCAG ACTGTAACTA CCAAGTACCT CCTGACCAAC 
CAGTCCCAGG GAACACTAAA GTTTGAGTGC TTCTATTTCA AGGAGGCTGG TGACTACTGG 
TTCACAATGA CTCCAGAAGC AACAGACAAC AGCACTCCAT TCCCCTGGTG GGAGAAAAGT 
GCCTTTCTGA AGGTGGAATG GCCTGTCTTT CACGTTGACT TGAATAGGAG TGCCAAGGCA 
GCAGAAGGCA CCTTCCAAGT GGGCCTATTT ACCAGTCAAC CACTGTGCCC GTTTCCTGTG 
GACAAGCCCA ACATCGTAGT GGATGTCATC TTCACCAACA GTCTTCCTGA GGCAAGAAGA 
AATTCAAGAC AGCCGCTGGA AATAAGAACC AGCAAAAGGA CAGAACTTGC TCAAGGTCAG 
TGGGTTGAGT TTGGCTGTGC ACCCTTGGGG CCAGAAGCCT ATGTCACCGT GGTGCTGAAG 
CTGCTTGGGC GAGACTCAGT CATTACCTCC ACAGGACCCA TTGACCTGGC CCAGAAATTT 
GGATACAAAC TGGTGATGGT GCCAGAACTC ACATGTGAGT CCGGGGTAGA GGTGACAGTG 
CTGCCTCCAC CATGCACCTT CGTCCAAGGA GTGGTCACTG TCTTCAAGGA GGCCCCCAGA 
TACCCTGGGA AGAGGACCAT TCACTTGGCT GAAAACAGCC TGCCCCTGGG AGAGAGGAGG 
ACAATTTTTA ACTGTACTTT GTTTGACATG GGGAAGAATA AGTACTGCTT TGACTTTGGC 
ATTTCAAGCA GAAGCCATTT TTCTGCAAAG GAGGAGTGCA TGCTAATTCA GAGAAATACA 
GAAACTTGGG GACTGTGGCA GCCATGGAGC CAGTGTAGTG CCACATGTGG GGATGGTGTC 
AGAGAGCGTC GCCGAGTGTG TCTCACTTCC TTCCCCTCCA GTCCTGTCTG CCCTGGAATG 
TCCTTGGAGG CCTCCCTGTG TTCCCTGGAG GAGTGTGCTG CTTTCCAGCC ATCCAGCCCA 
TCTCCTCTTC AGCCCCAGGG TCCAGTGAAG TCCAACAACA TCGTGACTGT CACTGGTATA 
TCCTTGTGCT TGTTCATCAT CATTGCCACT GTGCTCATCA CGCTGTGGAG GAGGTTCGGC 
CGGCCAGCCA AGTGCAGCAC ACCTGCTCGA CACAACTCCA TCCACTCCCC CAGCTTCCGG 
AAGAACTCGG ACGAGGAGAA TATCTGCGAG CTGAGCGAGC AGCGCGGGAG CTTCTCGGAT 
GGGGGAGACG GGCCCACGGG GAGTCCAGGG GACACAGGCA TCCCTCTGAC CTACAGGCGG 
AGCGGGCCGG TACCTCCCGA GGATGATGCC TCTGGCAGCG AGAGCTTCCA GTCCAACGCC 
CAGAAGATAA TCCCACCTCT GTTCAGCTAC CGCCTTGCCC AGCAGCAGTT AAAGGAGATG 
AAAAAGAAAG GTCTGACGGA AACTACCAAA GTGTATCACG TGTCTCAGAG TCCCCTGACA 
GACACTGCCA TTGATGCGGC CCCCAGCGCT CCCTTAGATT TGGAAAGCCC GGAAGAAGCT 
GCAGCAAACA AGTTCCGGAT CAAATCCCCA TTTCCGGAGC AGCCCGCGGT CAGTGCCGGG 
GAAAGGCCTC CCTCCAGGCT GGATCTAAAT GTGACTCAGG CCAGTTGTGC CATAAGCCCC 
AGCCAGACTC TGATCCGCAA GTCACAGGCA AGGCACGTGG GCAGCAGAGG GGGCCCGTCC 
GAAAGGAGCC ATGCCAGGAA CGCCCATTTC AGGAGGACAG CGAGTTTCCA TGAAGCCAGG 
CAGGCCCGGC CGTTCCGAGA GAGGAGCATG TCCACTCTGA CTCCACGGCA GGCCCCTGCC 
TACAGCTCTA GGACGCGGAC CTGCGAGCAG GCAGAGGACA GATTTAGGCC TCAGAGTCGA 
GGTGCCCACC TGTTTCCTGA AAAACTGGAG CATTTCCAAG AGGCAAGTGG AACCCGTGGT 
CCATTAAACC CTCTCCCTAA ATCCTACACT TTGGGGCAGC CCTTGAGGAA ACCAGACCTT 
GGGGATCACC AGGCAGGATT AGTGGCCGGA ATTGAGAGAA CAGAGCCCCA CAGAGCTCGT 
CGGGGACCGT CCCCCAGTCA CAAGAGTGTC TCAAGGAAGC AGTCTTCTCC CATATCCCCC 
AAAGATAACT ACCAGAGGGT CAGTTCTCTG AGCCCTTCTC AGTGTAGAAA AGACAAGTGT 
CAAAGCTTCC CCACTCACCC TGAGTTTGCC TTCTATGACA ATACGTCGTT TGGCCTCACT 
GAGGCTGAGC AGAGGATGCT GGACCTCCCA GGATATTTTG GGTCAAATGA AGAGGATGAA 
ACCACAAGTA CACTTAGCGT GGAGAAGCTG GTGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THSD1 ( NM_018676.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_061146.1 Homo sapiens thrombospondin type 1 domain containing 1 (THSD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08667
Clone ID Related Accession (Same CDS sequence)	NM_018676.3 , NM_018676.4
Accession Version	NM_018676.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Homo sapiens(human)
Product	thrombospondin type-1 domain-containing protein 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096289.1, AL359513.12 and BC063842.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_018676.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.226789.1, SRR1803612.241247.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000258613.5/ ENSP00000258613.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAACCAA TGTTGAAAGA CTTTTCAAAT CTATTGTTGG TGGTACTCTG TGACTATGTT 
CTTGGAGAAG CTGAATATCT TCTCTTGAGA GAGCCAGGCC ATGTAGCACT AAGCAACGAC 
ACAGTGTATG TGGATTTCCA GTATTTTGAT GGTGCTAATG GGACACTGAG GAATGTATCT 
GTCCTGCTGT TGGAGGCCAA CACCAATCAG ACTGTAACTA CCAAGTACCT CCTGACCAAC 
CAGTCCCAGG GAACACTAAA GTTTGAGTGC TTCTATTTCA AGGAGGCTGG TGACTACTGG 
TTCACAATGA CTCCAGAAGC AACAGACAAC AGCACTCCAT TCCCCTGGTG GGAGAAAAGT 
GCCTTTCTGA AGGTGGAATG GCCTGTCTTT CACGTTGACT TGAATAGGAG TGCCAAGGCA 
GCAGAAGGCA CCTTCCAAGT GGGCCTATTT ACCAGTCAAC CACTGTGCCC GTTTCCTGTG 
GACAAGCCCA ACATCGTAGT GGATGTCATC TTCACCAACA GTCTTCCTGA GGCAAGAAGA 
AATTCAAGAC AGCCGCTGGA AATAAGAACC AGCAAAAGGA CAGAACTTGC TCAAGGTCAG 
TGGGTTGAGT TTGGCTGTGC ACCCTTGGGG CCAGAAGCCT ATGTCACCGT GGTGCTGAAG 
CTGCTTGGGC GAGACTCAGT CATTACCTCC ACAGGACCCA TTGACCTGGC CCAGAAATTT 
GGATACAAAC TGGTGATGGT GCCAGAACTC ACATGTGAGT CCGGGGTAGA GGTGACAGTG 
CTGCCTCCAC CATGCACCTT CGTCCAAGGA GTGGTCACTG TCTTCAAGGA GGCCCCCAGA 
TACCCTGGGA AGAGGACCAT TCACTTGGCT GAAAACAGCC TGCCCCTGGG AGAGAGGAGG 
ACAATTTTTA ACTGTACTTT GTTTGACATG GGGAAGAATA AGTACTGCTT TGACTTTGGC 
ATTTCAAGCA GAAGCCATTT TTCTGCAAAG GAGGAGTGCA TGCTAATTCA GAGAAATACA 
GAAACTTGGG GACTGTGGCA GCCATGGAGC CAGTGTAGTG CCACATGTGG GGATGGTGTC 
AGAGAGCGTC GCCGAGTGTG TCTCACTTCC TTCCCCTCCA GTCCTGTCTG CCCTGGAATG 
TCCTTGGAGG CCTCCCTGTG TTCCCTGGAG GAGTGTGCTG CTTTCCAGCC ATCCAGCCCA 
TCTCCTCTTC AGCCCCAGGG TCCAGTGAAG TCCAACAACA TCGTGACTGT CACTGGTATA 
TCCTTGTGCT TGTTCATCAT CATTGCCACT GTGCTCATCA CGCTGTGGAG GAGGTTCGGC 
CGGCCAGCCA AGTGCAGCAC ACCTGCTCGA CACAACTCCA TCCACTCCCC CAGCTTCCGG 
AAGAACTCGG ACGAGGAGAA TATCTGCGAG CTGAGCGAGC AGCGCGGGAG CTTCTCGGAT 
GGGGGAGACG GGCCCACGGG GAGTCCAGGG GACACAGGCA TCCCTCTGAC CTACAGGCGG 
AGCGGGCCGG TACCTCCCGA GGATGATGCC TCTGGCAGCG AGAGCTTCCA GTCCAACGCC 
CAGAAGATAA TCCCACCTCT GTTCAGCTAC CGCCTTGCCC AGCAGCAGTT AAAGGAGATG 
AAAAAGAAAG GTCTGACGGA AACTACCAAA GTGTATCACG TGTCTCAGAG TCCCCTGACA 
GACACTGCCA TTGATGCGGC CCCCAGCGCT CCCTTAGATT TGGAAAGCCC GGAAGAAGCT 
GCAGCAAACA AGTTCCGGAT CAAATCCCCA TTTCCGGAGC AGCCCGCGGT CAGTGCCGGG 
GAAAGGCCTC CCTCCAGGCT GGATCTAAAT GTGACTCAGG CCAGTTGTGC CATAAGCCCC 
AGCCAGACTC TGATCCGCAA GTCACAGGCA AGGCACGTGG GCAGCAGAGG GGGCCCGTCC 
GAAAGGAGCC ATGCCAGGAA CGCCCATTTC AGGAGGACAG CGAGTTTCCA TGAAGCCAGG 
CAGGCCCGGC CGTTCCGAGA GAGGAGCATG TCCACTCTGA CTCCACGGCA GGCCCCTGCC 
TACAGCTCTA GGACGCGGAC CTGCGAGCAG GCAGAGGACA GATTTAGGCC TCAGAGTCGA 
GGTGCCCACC TGTTTCCTGA AAAACTGGAG CATTTCCAAG AGGCAAGTGG AACCCGTGGT 
CCATTAAACC CTCTCCCTAA ATCCTACACT TTGGGGCAGC CCTTGAGGAA ACCAGACCTT 
GGGGATCACC AGGCAGGATT AGTGGCCGGA ATTGAGAGAA CAGAGCCCCA CAGAGCTCGT 
CGGGGACCGT CCCCCAGTCA CAAGAGTGTC TCAAGGAAGC AGTCTTCTCC CATATCCCCC 
AAAGATAACT ACCAGAGGGT CAGTTCTCTG AGCCCTTCTC AGTGTAGAAA AGACAAGTGT 
CAAAGCTTCC CCACTCACCC TGAGTTTGCC TTCTATGACA ATACGTCGTT TGGCCTCACT 
GAGGCTGAGC AGAGGATGCT GGACCTCCCA GGATATTTTG GGTCAAATGA AGAGGATGAA 
ACCACAAGTA CACTTAGCGT GGAGAAGCTG GTGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061146.1
CDS	224..2782
Translation	MKPMLKDFSNLLLVVLCDYVLGEAEYLLLREPGHVALSNDTVYVDFQYFDGANGTLRNVSVLLLEANTNQTVTTKYLLTNQSQGTLKFECFYFKEAGDYWFTMTPEATDNSTPFPWWEKSAFLKVEWPVFHVDLNRSAKAAEGTFQVGLFTSQPLCPFPVDKPNIVVDVIFTNSLPEARRNSRQPLEIRTSKRTELAQGQWVEFGCAPLGPEAYVTVVLKLLGRDSVITSTGPIDLAQKFGYKLVMVPELTCESGVEVTVLPPPCTFVQGVVTVFKEAPRYPGKRTIHLAENSLPLGERRTIFNCTLFDMGKNKYCFDFGISSRSHFSAKEECMLIQRNTETWGLWQPWSQCSATCGDGVRERRRVCLTSFPSSPVCPGMSLEASLCSLEECAAFQPSSPSPLQPQGPVKSNNIVTVTGISLCLFIIIATVLITLWRRFGRPAKCSTPARHNSIHSPSFRKNSDEENICELSEQRGSFSDGGDGPTGSPGDTGIPLTYRRSGPVPPEDDASGSESFQSNAQKIIPPLFSYRLAQQQLKEMKKKGLTETTKVYHVSQSPLTDTAIDAAPSAPLDLESPEEAAANKFRIKSPFPEQPAVSAGERPPSRLDLNVTQASCAISPSQTLIRKSQARHVGSRGGPSERSHARNAHFRRTASFHEARQARPFRERSMSTLTPRQAPAYSSRTRTCEQAEDRFRPQSRGAHLFPEKLEHFQEASGTRGPLNPLPKSYTLGQPLRKPDLGDHQAGLVAGIERTEPHRARRGPSPSHKSVSRKQSSPISPKDNYQRVSSLSPSQCRKDKCQSFPTHPEFAFYDNTSFGLTEAEQRMLDLPGYFGSNEEDETTSTLSVEKLVI

Target ORF information:

RefSeq Version	NM_018676.4
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type 1 domain containing 1 (THSD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018676.4

ORF Insert Sequence:

ATGAAACCAA TGTTGAAAGA CTTTTCAAAT CTATTGTTGG TGGTACTCTG TGACTATGTT 
CTTGGAGAAG CTGAATATCT TCTCTTGAGA GAGCCAGGCC ATGTAGCACT AAGCAACGAC 
ACAGTGTATG TGGATTTCCA GTATTTTGAT GGTGCTAATG GGACACTGAG GAATGTATCT 
GTCCTGCTGT TGGAGGCCAA CACCAATCAG ACTGTAACTA CCAAGTACCT CCTGACCAAC 
CAGTCCCAGG GAACACTAAA GTTTGAGTGC TTCTATTTCA AGGAGGCTGG TGACTACTGG 
TTCACAATGA CTCCAGAAGC AACAGACAAC AGCACTCCAT TCCCCTGGTG GGAGAAAAGT 
GCCTTTCTGA AGGTGGAATG GCCTGTCTTT CACGTTGACT TGAATAGGAG TGCCAAGGCA 
GCAGAAGGCA CCTTCCAAGT GGGCCTATTT ACCAGTCAAC CACTGTGCCC GTTTCCTGTG 
GACAAGCCCA ACATCGTAGT GGATGTCATC TTCACCAACA GTCTTCCTGA GGCAAGAAGA 
AATTCAAGAC AGCCGCTGGA AATAAGAACC AGCAAAAGGA CAGAACTTGC TCAAGGTCAG 
TGGGTTGAGT TTGGCTGTGC ACCCTTGGGG CCAGAAGCCT ATGTCACCGT GGTGCTGAAG 
CTGCTTGGGC GAGACTCAGT CATTACCTCC ACAGGACCCA TTGACCTGGC CCAGAAATTT 
GGATACAAAC TGGTGATGGT GCCAGAACTC ACATGTGAGT CCGGGGTAGA GGTGACAGTG 
CTGCCTCCAC CATGCACCTT CGTCCAAGGA GTGGTCACTG TCTTCAAGGA GGCCCCCAGA 
TACCCTGGGA AGAGGACCAT TCACTTGGCT GAAAACAGCC TGCCCCTGGG AGAGAGGAGG 
ACAATTTTTA ACTGTACTTT GTTTGACATG GGGAAGAATA AGTACTGCTT TGACTTTGGC 
ATTTCAAGCA GAAGCCATTT TTCTGCAAAG GAGGAGTGCA TGCTAATTCA GAGAAATACA 
GAAACTTGGG GACTGTGGCA GCCATGGAGC CAGTGTAGTG CCACATGTGG GGATGGTGTC 
AGAGAGCGTC GCCGAGTGTG TCTCACTTCC TTCCCCTCCA GTCCTGTCTG CCCTGGAATG 
TCCTTGGAGG CCTCCCTGTG TTCCCTGGAG GAGTGTGCTG CTTTCCAGCC ATCCAGCCCA 
TCTCCTCTTC AGCCCCAGGG TCCAGTGAAG TCCAACAACA TCGTGACTGT CACTGGTATA 
TCCTTGTGCT TGTTCATCAT CATTGCCACT GTGCTCATCA CGCTGTGGAG GAGGTTCGGC 
CGGCCAGCCA AGTGCAGCAC ACCTGCTCGA CACAACTCCA TCCACTCCCC CAGCTTCCGG 
AAGAACTCGG ACGAGGAGAA TATCTGCGAG CTGAGCGAGC AGCGCGGGAG CTTCTCGGAT 
GGGGGAGACG GGCCCACGGG GAGTCCAGGG GACACAGGCA TCCCTCTGAC CTACAGGCGG 
AGCGGGCCGG TACCTCCCGA GGATGATGCC TCTGGCAGCG AGAGCTTCCA GTCCAACGCC 
CAGAAGATAA TCCCACCTCT GTTCAGCTAC CGCCTTGCCC AGCAGCAGTT AAAGGAGATG 
AAAAAGAAAG GTCTGACGGA AACTACCAAA GTGTATCACG TGTCTCAGAG TCCCCTGACA 
GACACTGCCA TTGATGCGGC CCCCAGCGCT CCCTTAGATT TGGAAAGCCC GGAAGAAGCT 
GCAGCAAACA AGTTCCGGAT CAAATCCCCA TTTCCGGAGC AGCCCGCGGT CAGTGCCGGG 
GAAAGGCCTC CCTCCAGGCT GGATCTAAAT GTGACTCAGG CCAGTTGTGC CATAAGCCCC 
AGCCAGACTC TGATCCGCAA GTCACAGGCA AGGCACGTGG GCAGCAGAGG GGGCCCGTCC 
GAAAGGAGCC ATGCCAGGAA CGCCCATTTC AGGAGGACAG CGAGTTTCCA TGAAGCCAGG 
CAGGCCCGGC CGTTCCGAGA GAGGAGCATG TCCACTCTGA CTCCACGGCA GGCCCCTGCC 
TACAGCTCTA GGACGCGGAC CTGCGAGCAG GCAGAGGACA GATTTAGGCC TCAGAGTCGA 
GGTGCCCACC TGTTTCCTGA AAAACTGGAG CATTTCCAAG AGGCAAGTGG AACCCGTGGT 
CCATTAAACC CTCTCCCTAA ATCCTACACT TTGGGGCAGC CCTTGAGGAA ACCAGACCTT 
GGGGATCACC AGGCAGGATT AGTGGCCGGA ATTGAGAGAA CAGAGCCCCA CAGAGCTCGT 
CGGGGACCGT CCCCCAGTCA CAAGAGTGTC TCAAGGAAGC AGTCTTCTCC CATATCCCCC 
AAAGATAACT ACCAGAGGGT CAGTTCTCTG AGCCCTTCTC AGTGTAGAAA AGACAAGTGT 
CAAAGCTTCC CCACTCACCC TGAGTTTGCC TTCTATGACA ATACGTCGTT TGGCCTCACT 
GAGGCTGAGC AGAGGATGCT GGACCTCCCA GGATATTTTG GGTCAAATGA AGAGGATGAA 
ACCACAAGTA CACTTAGCGT GGAGAAGCTG GTGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Intracranial Aneurysm Gene THSD1 Connects Endosome Dynamics to Nascent Focal Adhesion Assembly.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology43(6)2200-2211(2017)
Rui YN,Xu Z,Fang X,Menezes MR,Balzeau J,Niu A,Hagan JP,Kim DH

THSD1 (Thrombospondin Type 1 Domain Containing Protein 1) Mutation in the Pathogenesis of Intracranial Aneurysm and Subarachnoid Hemorrhage.
Stroke47(12)3005-3013(2016 Dec)
Santiago-Sim T,Fang X,Hennessy ML,Nalbach SV,DePalma SR,Lee MS,Greenway SC,McDonough B,Hergenroeder GW,Patek KJ,Colosimo SM,Qualmann KJ,Hagan JP,Milewicz DM,MacRae CA,Dymecki SM,Seidman CE,Seidman JG,Kim DH

THSD1 preserves vascular integrity and protects against intraplaque haemorrhaging in ApoE-/- mice.
Cardiovascular research110(1)129-39(2016 May)
Haasdijk RA,Den Dekker WK,Cheng C,Tempel D,Szulcek R,Bos FL,Hermkens DM,Chrifi I,Brandt MM,Van Dijk C,Xu YJ,Van De Kamp EH,Blonden LA,Van Bezu J,Sluimer JC,Biessen EA,Van Nieuw Amerongen GP,Duckers HJ

Genome-wide screening for methylation-silenced genes in colorectal cancer.
International journal of oncology41(2)490-6(2012 Aug)
Khamas A,Ishikawa T,Mogushi K,Iida S,Ishiguro M,Tanaka H,Uetake H,Sugihara K

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THSD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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