CDKN2AIP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDKN2AIP
Entrez Gene ID	55602
Full Name	CDKN2A interacting protein
Synonyms	CARF
General protein information	Preferred Names CDKN2A interacting protein Names CDKN2A-interacting protein collaborates/cooperates with ARF (alternate reading frame) protein collaborator of ARF
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q35.1
Summary	The protein encoded by this gene regulates the DNA damage response through several different signaling pathways. One such pathway is the p53-HDM2-p21(WAF1) pathway, which is critical to the DNA damage response. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015].
Disorder MIM:	615914

mRNA and Protein(s)

mRNA	Protein	Name
NM_001317343.2	NP_001304272.1	CDKN2A-interacting protein isoform 2
NM_001317343.1	NP_001304272.1	CDKN2A-interacting protein isoform 2
NM_017632.4	NP_060102.1	CDKN2A-interacting protein isoform 1
NM_017632.3	NP_060102.1	CDKN2A-interacting protein isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CDKN2AIP	XP_849962.1
Bos taurus (cattle)	CDKN2AIP	NP_001039608.2
Homo sapiens (human)	CDKN2AIP	NP_060102.1
Mus musculus (house mouse)	Cdkn2aip	NP_765995.1
	CDKN2AIP	XP_001093046.1
Pan troglodytes (chimpanzee)	CDKN2AIP	XP_517548.2
Rattus norvegicus (Norway rat)	Cdkn2aip	NP_001014022.1
Danio rerio (zebrafish)	cdkn2aip	NP_001006085.1
Xenopus tropicalis (tropical clawed frog)	cdkn2aip	XP_002934305.1

Gene Ontology
Bibliography

Related articles in PubMed

CARF is a multi-module regulator of cell proliferation and a molecular bridge between cellular senescence and carcinogenesis.
Wadhwa R, Kalra RS, Kaul SC
Mechanisms of ageing and development16664-68(2017 Sep)

CARF activates beta-catenin/TCF signaling in the hepatocellular carcinoma.
Fan X, Ma X, Cui L, Dang S, Qu J, Zhang J, Wang X, Mao Z
Oncotarget7(49)80404-80414(2016 Dec)

Loss-of-function screening to identify miRNAs involved in senescence: tumor suppressor activity of miRNA-335 and its new target CARF.
Yu Y, Gao R, Kaul Z, Li L, Kato Y, Zhang Z, Groden J, Kaul SC, Wadhwa R
Scientific reports630185(2016 Jul)

Collaborator of alternative reading frame protein (CARF) regulates early processing of pre-ribosomal RNA by retaining XRN2 (5'-3' exoribonuclease) in the nucleoplasm.
Sato S, Ishikawa H, Yoshikawa H, Izumikawa K, Simpson RJ, Takahashi N
Nucleic acids research43(21)10397-410(2015 Dec)

CARF (Collaborator of ARF) overexpression in p53-deficient cells promotes carcinogenesis.
Kalra RS, Cheung CT, Chaudhary A, Prakash J, Kaul SC, Wadhwa R
Molecular oncology9(9)1877-89(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that CARF regulates early steps of pre-rRNA processing during ribosome biogenesis by controlling spatial distribution of XRN2 between the nucleoplasm and nucleolus.
Title: Collaborator of alternative reading frame protein (CARF) regulates early processing of pre-ribosomal RNA by retaining XRN2 (5'-3' exoribonuclease) in the nucleoplasm.

CARF promotes carcinogenesis in p53-deficient cells by repressing p21WAF1 and promoting cell cycle progression.
Title: CARF (Collaborator of ARF) overexpression in p53-deficient cells promotes carcinogenesis.

CARF regulates proliferative fate of human cells by dose-dependent regulation of DNA damage signaling.
Title: Collaborator of ARF (CARF) regulates proliferative fate of human cells by dose-dependent regulation of DNA damage signaling.

Analysis of indel variations in the human disease-associated genes CDKN2AIP, WDR66, USP20 and OR7C2 in a Korean population.
Title: Analysis of indel variations in the human disease-associated genes CDKN2AIP, WDR66, USP20 and OR7C2 in a Korean population.

CARF knockdown elicited DNA damage response as evidenced by increased levels of phosphorylated ATM and gammaH2AX, leading to induction of mitotic arrest and eventual apoptosis.
Title: Molecular characterization of apoptosis induced by CARF silencing in human cancer cells.

The following CDKN2AIP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDKN2AIP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02877	NM_017632.4 Latest version!	Homo sapiens CDKN2A interacting protein (CDKN2AIP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu101496	NM_001317343.2 Latest version!	Homo sapiens CDKN2A interacting protein (CDKN2AIP), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu101496	NM_001317343.1	Homo sapiens CDKN2A interacting protein (CDKN2AIP), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu02877	NM_017632.3	Homo sapiens CDKN2A interacting protein (CDKN2AIP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDKN2AIP ( NM_017632.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060102.1 Homo sapiens CDKN2A interacting protein (CDKN2AIP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02877
Clone ID Related Accession (Same CDS sequence)	NM_017632.4 , NM_017632.3
Accession Version	NM_017632.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	CDKN2A-interacting protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK000043.1, AF246705.1, AC112698.4 and AA622329.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_017632.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.42038.1, SRR1803614.156064.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000504169.2/ ENSP00000427108.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGCAGG AGGTGTCGGA GTACCTGAGC CAGAACCCGC GGGTGGCAGC CTGGGTGGAG 
GCGCTGCGCT GCGACGGCGA GACTGACAAA CACTGGCGCC ACCGCCGGGA TTTTTTGCTT 
CGCAACGCCG GGGACCTGGC CCCCGCTGGC GGCGCTGCCT CCGCTAGCAC GGATGAAGCT 
GCCGACGCCG AGAGCGGGAC CCGAAACCGG CAGCTGCAGC AGCTCATCTC CTTTTCCATG 
GCCTGGGCGA ACCACGTCTT CCTCGGGTGC CGATACCCTC AAAAAGTTAT GGATAAAATA 
CTTAGTATGG CTGAAGGCAT CAAAGTGACA GATGCTCCAA CCTATACAAC AAGAGATGAA 
CTGGTTGCCA AGGTGAAGAA AAGAGGGATA TCGAGTAGCA ATGAAGGGGT AGAAGAGCCA 
TCCAAAAAAC GAGTTATAGA AGGAAAAAAC AGTTCTGCAG TTGAGCAAGA TCACGCAAAA 
ACCTCTGCCA AGACAGAACG TGCATCAGCT CAGCAGGAAA ACAGTTCAAC GTGTATAGGG 
TCGGCCATCA AATCAGAGAG TGGGAACTCA GCTCGGAGCT CTGGCATCTC CAGTCAGAAT 
AGCTCTACAA GTGATGGAGA TCGATCTGTT TCCAGCCAAA GCAGCAGCAG CGTTTCCTCT 
CAGGTAACAA CGGCAGGATC TGGGAAAGCT TCTGAAGCAG AAGCTCCAGA TAAACACGGT 
TCTGCATCAT TTGTTTCCTT GCTGAAATCC AGTGTGAATA GTCACATGAC CCAATCCACT 
GATTCTAGAC AACAAAGTGG ATCACCTAAA AAGAGTGCTT TGGAAGGCTC TTCAGCCTCA 
GCTTCTCAAA GCAGCTCAGA GATCGAGGTG CCCTTGTTGG GCTCCTCAGG AAGCTCAGAG 
GTAGAATTGC CACTATTGTC TTCCAAACCT AGTTCAGAGA CAGCTTCAAG TGGGTTAACT 
TCCAAAACTA GTTCAGAGGC AAGTGTTTCA TCATCAGTTG CTAAAAACAG TTCCTCATCA 
GGCACATCCT TACTGACTCC CAAGAGCAGC TCTTCAACAA ATACATCGCT GCTAACTTCC 
AAGAGCACTT CCCAGGTAGC TGCATCACTA CTAGCTTCCA AGAGCAGCTC CCAGACCAGT 
GGATCTCTGG TTTCCAAAAG CACTTCCTTA GCAAGTGTGT CCCAGTTGGC TTCTAAGAGT 
AGTTCTCAGA CTAGCACCTC ACAGTTGCCT TCTAAAAGTA CTTCACAGTC AAGTGAGAGT 
TCTGTCAAAT TCTCTTGCAA GTTAACCAAT GAAGATGTGA AACAGAAGCA ACCTTTTTTC 
AATAGACTAT ATAAAACGGT GGCATGGAAG TTGGTAGCTG TTGGTGGCTT TAGTCCCAAT 
GTGAATCATG GAGAGCTCCT AAATGCAGCT ATTGAGGCTC TGAAAGCAAC ACTGGATGTA 
TTTTTTGTCC CACTAAAAGA ATTGGCAGAT CTGCCTCAAA ATAAGAGCTC TCAAGAAAGT 
ATTGTTTGTG AATTGAGGTG CAAGTCTGTG TATTTGGGCA CTGGCTGTGG AAAAAGCAAA 
GAAAATGCAA AAGCAGTTGC ATCAAGAGAA GCATTGAAGT TATTTCTCAA GAAAAAGGTG 
GTGGTAAAAA TATGTAAAAG GAAATACAGA GGCAGTGAAA TAGAAGATCT AGTACTCCTT 
GATGAAGAAT CGAGGCCTGT AAACTTACCT CCAGCACTAA AACATCCTCA AGAATTACTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060102.1
CDS	163..1905
Translation	MAQEVSEYLSQNPRVAAWVEALRCDGETDKHWRHRRDFLLRNAGDLAPAGGAASASTDEAADAESGTRNRQLQQLISFSMAWANHVFLGCRYPQKVMDKILSMAEGIKVTDAPTYTTRDELVAKVKKRGISSSNEGVEEPSKKRVIEGKNSSAVEQDHAKTSAKTERASAQQENSSTCIGSAIKSESGNSARSSGISSQNSSTSDGDRSVSSQSSSSVSSQVTTAGSGKASEAEAPDKHGSASFVSLLKSSVNSHMTQSTDSRQQSGSPKKSALEGSSASASQSSSEIEVPLLGSSGSSEVELPLLSSKPSSETASSGLTSKTSSEASVSSSVAKNSSSSGTSLLTPKSSSSTNTSLLTSKSTSQVAASLLASKSSSQTSGSLVSKSTSLASVSQLASKSSSQTSTSQLPSKSTSQSSESSVKFSCKLTNEDVKQKQPFFNRLYKTVAWKLVAVGGFSPNVNHGELLNAAIEALKATLDVFFVPLKELADLPQNKSSQESIVCELRCKSVYLGTGCGKSKENAKAVASREALKLFLKKKVVVKICKRKYRGSEIEDLVLLDEESRPVNLPPALKHPQELL

Target ORF information:

RefSeq Version	NM_017632.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CDKN2A interacting protein (CDKN2AIP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017632.4

ORF Insert Sequence:

ATGGCGCAGG AGGTGTCGGA GTACCTGAGC CAGAACCCGC GGGTGGCAGC CTGGGTGGAG 
GCGCTGCGCT GCGACGGCGA GACTGACAAA CACTGGCGCC ACCGCCGGGA TTTTTTGCTT 
CGCAACGCCG GGGACCTGGC CCCCGCTGGC GGCGCTGCCT CCGCTAGCAC GGATGAAGCT 
GCCGACGCCG AGAGCGGGAC CCGAAACCGG CAGCTGCAGC AGCTCATCTC CTTTTCCATG 
GCCTGGGCGA ACCACGTCTT CCTCGGGTGC CGATACCCTC AAAAAGTTAT GGATAAAATA 
CTTAGTATGG CTGAAGGCAT CAAAGTGACA GATGCTCCAA CCTATACAAC AAGAGATGAA 
CTGGTTGCCA AGGTGAAGAA AAGAGGGATA TCGAGTAGCA ATGAAGGGGT AGAAGAGCCA 
TCCAAAAAAC GAGTTATAGA AGGAAAAAAC AGTTCTGCAG TTGAGCAAGA TCACGCAAAA 
ACCTCTGCCA AGACAGAACG TGCATCAGCT CAGCAGGAAA ACAGTTCAAC GTGTATAGGG 
TCGGCCATCA AATCAGAGAG TGGGAACTCA GCTCGGAGCT CTGGCATCTC CAGTCAGAAT 
AGCTCTACAA GTGATGGAGA TCGATCTGTT TCCAGCCAAA GCAGCAGCAG CGTTTCCTCT 
CAGGTAACAA CGGCAGGATC TGGGAAAGCT TCTGAAGCAG AAGCTCCAGA TAAACACGGT 
TCTGCATCAT TTGTTTCCTT GCTGAAATCC AGTGTGAATA GTCACATGAC CCAATCCACT 
GATTCTAGAC AACAAAGTGG ATCACCTAAA AAGAGTGCTT TGGAAGGCTC TTCAGCCTCA 
GCTTCTCAAA GCAGCTCAGA GATCGAGGTG CCCTTGTTGG GCTCCTCAGG AAGCTCAGAG 
GTAGAATTGC CACTATTGTC TTCCAAACCT AGTTCAGAGA CAGCTTCAAG TGGGTTAACT 
TCCAAAACTA GTTCAGAGGC AAGTGTTTCA TCATCAGTTG CTAAAAACAG TTCCTCATCA 
GGCACATCCT TACTGACTCC CAAGAGCAGC TCTTCAACAA ATACATCGCT GCTAACTTCC 
AAGAGCACTT CCCAGGTAGC TGCATCACTA CTAGCTTCCA AGAGCAGCTC CCAGACCAGT 
GGATCTCTGG TTTCCAAAAG CACTTCCTTA GCAAGTGTGT CCCAGTTGGC TTCTAAGAGT 
AGTTCTCAGA CTAGCACCTC ACAGTTGCCT TCTAAAAGTA CTTCACAGTC AAGTGAGAGT 
TCTGTCAAAT TCTCTTGCAA GTTAACCAAT GAAGATGTGA AACAGAAGCA ACCTTTTTTC 
AATAGACTAT ATAAAACGGT GGCATGGAAG TTGGTAGCTG TTGGTGGCTT TAGTCCCAAT 
GTGAATCATG GAGAGCTCCT AAATGCAGCT ATTGAGGCTC TGAAAGCAAC ACTGGATGTA 
TTTTTTGTCC CACTAAAAGA ATTGGCAGAT CTGCCTCAAA ATAAGAGCTC TCAAGAAAGT 
ATTGTTTGTG AATTGAGGTG CAAGTCTGTG TATTTGGGCA CTGGCTGTGG AAAAAGCAAA 
GAAAATGCAA AAGCAGTTGC ATCAAGAGAA GCATTGAAGT TATTTCTCAA GAAAAAGGTG 
GTGGTAAAAA TATGTAAAAG GAAATACAGA GGCAGTGAAA TAGAAGATCT AGTACTCCTT 
GATGAAGAAT CGAGGCCTGT AAACTTACCT CCAGCACTAA AACATCCTCA AGAATTACTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDKN2AIP ( NM_017632.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060102.1 Homo sapiens CDKN2A interacting protein (CDKN2AIP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02877
Clone ID Related Accession (Same CDS sequence)	NM_017632.4 , NM_017632.3
Accession Version	NM_017632.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	CDKN2A-interacting protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN427056.1, AK000043.1, AF246705.1, AC112698.4 and AA622329.1. This sequence is a reference standard in the RefSeqGene project. On Nov 26, 2015 this sequence version replaced NM_017632.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CN427056.1, SRR1803614.156064.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCAGG AGGTGTCGGA GTACCTGAGC CAGAACCCGC GGGTGGCAGC CTGGGTGGAG 
GCGCTGCGCT GCGACGGCGA GACTGACAAA CACTGGCGCC ACCGCCGGGA TTTTTTGCTT 
CGCAACGCCG GGGACCTGGC CCCCGCTGGC GGCGCTGCCT CCGCTAGCAC GGATGAAGCT 
GCCGACGCCG AGAGCGGGAC CCGAAACCGG CAGCTGCAGC AGCTCATCTC CTTTTCCATG 
GCCTGGGCGA ACCACGTCTT CCTCGGGTGC CGATACCCTC AAAAAGTTAT GGATAAAATA 
CTTAGTATGG CTGAAGGCAT CAAAGTGACA GATGCTCCAA CCTATACAAC AAGAGATGAA 
CTGGTTGCCA AGGTGAAGAA AAGAGGGATA TCGAGTAGCA ATGAAGGGGT AGAAGAGCCA 
TCCAAAAAAC GAGTTATAGA AGGAAAAAAC AGTTCTGCAG TTGAGCAAGA TCACGCAAAA 
ACCTCTGCCA AGACAGAACG TGCATCAGCT CAGCAGGAAA ACAGTTCAAC GTGTATAGGG 
TCGGCCATCA AATCAGAGAG TGGGAACTCA GCTCGGAGCT CTGGCATCTC CAGTCAGAAT 
AGCTCTACAA GTGATGGAGA TCGATCTGTT TCCAGCCAAA GCAGCAGCAG CGTTTCCTCT 
CAGGTAACAA CGGCAGGATC TGGGAAAGCT TCTGAAGCAG AAGCTCCAGA TAAACACGGT 
TCTGCATCAT TTGTTTCCTT GCTGAAATCC AGTGTGAATA GTCACATGAC CCAATCCACT 
GATTCTAGAC AACAAAGTGG ATCACCTAAA AAGAGTGCTT TGGAAGGCTC TTCAGCCTCA 
GCTTCTCAAA GCAGCTCAGA GATCGAGGTG CCCTTGTTGG GCTCCTCAGG AAGCTCAGAG 
GTAGAATTGC CACTATTGTC TTCCAAACCT AGTTCAGAGA CAGCTTCAAG TGGGTTAACT 
TCCAAAACTA GTTCAGAGGC AAGTGTTTCA TCATCAGTTG CTAAAAACAG TTCCTCATCA 
GGCACATCCT TACTGACTCC CAAGAGCAGC TCTTCAACAA ATACATCGCT GCTAACTTCC 
AAGAGCACTT CCCAGGTAGC TGCATCACTA CTAGCTTCCA AGAGCAGCTC CCAGACCAGT 
GGATCTCTGG TTTCCAAAAG CACTTCCTTA GCAAGTGTGT CCCAGTTGGC TTCTAAGAGT 
AGTTCTCAGA CTAGCACCTC ACAGTTGCCT TCTAAAAGTA CTTCACAGTC AAGTGAGAGT 
TCTGTCAAAT TCTCTTGCAA GTTAACCAAT GAAGATGTGA AACAGAAGCA ACCTTTTTTC 
AATAGACTAT ATAAAACGGT GGCATGGAAG TTGGTAGCTG TTGGTGGCTT TAGTCCCAAT 
GTGAATCATG GAGAGCTCCT AAATGCAGCT ATTGAGGCTC TGAAAGCAAC ACTGGATGTA 
TTTTTTGTCC CACTAAAAGA ATTGGCAGAT CTGCCTCAAA ATAAGAGCTC TCAAGAAAGT 
ATTGTTTGTG AATTGAGGTG CAAGTCTGTG TATTTGGGCA CTGGCTGTGG AAAAAGCAAA 
GAAAATGCAA AAGCAGTTGC ATCAAGAGAA GCATTGAAGT TATTTCTCAA GAAAAAGGTG 
GTGGTAAAAA TATGTAAAAG GAAATACAGA GGCAGTGAAA TAGAAGATCT AGTACTCCTT 
GATGAAGAAT CGAGGCCTGT AAACTTACCT CCAGCACTAA AACATCCTCA AGAATTACTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060102.1
CDS	208..1950
Translation	MAQEVSEYLSQNPRVAAWVEALRCDGETDKHWRHRRDFLLRNAGDLAPAGGAASASTDEAADAESGTRNRQLQQLISFSMAWANHVFLGCRYPQKVMDKILSMAEGIKVTDAPTYTTRDELVAKVKKRGISSSNEGVEEPSKKRVIEGKNSSAVEQDHAKTSAKTERASAQQENSSTCIGSAIKSESGNSARSSGISSQNSSTSDGDRSVSSQSSSSVSSQVTTAGSGKASEAEAPDKHGSASFVSLLKSSVNSHMTQSTDSRQQSGSPKKSALEGSSASASQSSSEIEVPLLGSSGSSEVELPLLSSKPSSETASSGLTSKTSSEASVSSSVAKNSSSSGTSLLTPKSSSSTNTSLLTSKSTSQVAASLLASKSSSQTSGSLVSKSTSLASVSQLASKSSSQTSTSQLPSKSTSQSSESSVKFSCKLTNEDVKQKQPFFNRLYKTVAWKLVAVGGFSPNVNHGELLNAAIEALKATLDVFFVPLKELADLPQNKSSQESIVCELRCKSVYLGTGCGKSKENAKAVASREALKLFLKKKVVVKICKRKYRGSEIEDLVLLDEESRPVNLPPALKHPQELL

Target ORF information:

RefSeq Version	NM_017632.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CDKN2A interacting protein (CDKN2AIP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017632.3

ORF Insert Sequence:

ATGGCGCAGG AGGTGTCGGA GTACCTGAGC CAGAACCCGC GGGTGGCAGC CTGGGTGGAG 
GCGCTGCGCT GCGACGGCGA GACTGACAAA CACTGGCGCC ACCGCCGGGA TTTTTTGCTT 
CGCAACGCCG GGGACCTGGC CCCCGCTGGC GGCGCTGCCT CCGCTAGCAC GGATGAAGCT 
GCCGACGCCG AGAGCGGGAC CCGAAACCGG CAGCTGCAGC AGCTCATCTC CTTTTCCATG 
GCCTGGGCGA ACCACGTCTT CCTCGGGTGC CGATACCCTC AAAAAGTTAT GGATAAAATA 
CTTAGTATGG CTGAAGGCAT CAAAGTGACA GATGCTCCAA CCTATACAAC AAGAGATGAA 
CTGGTTGCCA AGGTGAAGAA AAGAGGGATA TCGAGTAGCA ATGAAGGGGT AGAAGAGCCA 
TCCAAAAAAC GAGTTATAGA AGGAAAAAAC AGTTCTGCAG TTGAGCAAGA TCACGCAAAA 
ACCTCTGCCA AGACAGAACG TGCATCAGCT CAGCAGGAAA ACAGTTCAAC GTGTATAGGG 
TCGGCCATCA AATCAGAGAG TGGGAACTCA GCTCGGAGCT CTGGCATCTC CAGTCAGAAT 
AGCTCTACAA GTGATGGAGA TCGATCTGTT TCCAGCCAAA GCAGCAGCAG CGTTTCCTCT 
CAGGTAACAA CGGCAGGATC TGGGAAAGCT TCTGAAGCAG AAGCTCCAGA TAAACACGGT 
TCTGCATCAT TTGTTTCCTT GCTGAAATCC AGTGTGAATA GTCACATGAC CCAATCCACT 
GATTCTAGAC AACAAAGTGG ATCACCTAAA AAGAGTGCTT TGGAAGGCTC TTCAGCCTCA 
GCTTCTCAAA GCAGCTCAGA GATCGAGGTG CCCTTGTTGG GCTCCTCAGG AAGCTCAGAG 
GTAGAATTGC CACTATTGTC TTCCAAACCT AGTTCAGAGA CAGCTTCAAG TGGGTTAACT 
TCCAAAACTA GTTCAGAGGC AAGTGTTTCA TCATCAGTTG CTAAAAACAG TTCCTCATCA 
GGCACATCCT TACTGACTCC CAAGAGCAGC TCTTCAACAA ATACATCGCT GCTAACTTCC 
AAGAGCACTT CCCAGGTAGC TGCATCACTA CTAGCTTCCA AGAGCAGCTC CCAGACCAGT 
GGATCTCTGG TTTCCAAAAG CACTTCCTTA GCAAGTGTGT CCCAGTTGGC TTCTAAGAGT 
AGTTCTCAGA CTAGCACCTC ACAGTTGCCT TCTAAAAGTA CTTCACAGTC AAGTGAGAGT 
TCTGTCAAAT TCTCTTGCAA GTTAACCAAT GAAGATGTGA AACAGAAGCA ACCTTTTTTC 
AATAGACTAT ATAAAACGGT GGCATGGAAG TTGGTAGCTG TTGGTGGCTT TAGTCCCAAT 
GTGAATCATG GAGAGCTCCT AAATGCAGCT ATTGAGGCTC TGAAAGCAAC ACTGGATGTA 
TTTTTTGTCC CACTAAAAGA ATTGGCAGAT CTGCCTCAAA ATAAGAGCTC TCAAGAAAGT 
ATTGTTTGTG AATTGAGGTG CAAGTCTGTG TATTTGGGCA CTGGCTGTGG AAAAAGCAAA 
GAAAATGCAA AAGCAGTTGC ATCAAGAGAA GCATTGAAGT TATTTCTCAA GAAAAAGGTG 
GTGGTAAAAA TATGTAAAAG GAAATACAGA GGCAGTGAAA TAGAAGATCT AGTACTCCTT 
GATGAAGAAT CGAGGCCTGT AAACTTACCT CCAGCACTAA AACATCCTCA AGAATTACTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDKN2AIP ( NM_001317343.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304272.1 Homo sapiens CDKN2A interacting protein (CDKN2AIP), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101496
Clone ID Related Accession (Same CDS sequence)	NM_001317343.2 , NM_001317343.1
Accession Version	NM_001317343.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	CDKN2A-interacting protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN427056.1, AK096180.1, AK000043.1, AF246705.1, AC112698.4 and AA622329.1. On May 31, 2019 this sequence version replaced NM_001317343.1. Transcript Variant: This variant (2) uses an alternate splice junction at the 3' end of an exon compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.184460.1, SRR1803612.95360.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCAGG AGGTGTCGGA GTACCTGAGC CAGAACCCGC GGGTGGCAGC CTGGGTGGAG 
GCGCTGCGCT GCGACGGCGA GACTGACAAA CACTGGCGCC ACCGCCGGGA TTTTTTGCTT 
CGCAACGCCG GGGACCTGGC CCCCGCTGGC GGCGCTGCCT CCGCTAGCAC GGATGAAGCT 
GCCGACGCCG AGAGCGGGAC CCGAAACCGG CAGCTGCAGC AGCTCATCTC CTTTTCCATG 
GCCTGGGCGA ACCACGTCTT CCTCGGGTGC CGATACCCTC AAAAAGTTAT GGATAAAATA 
CTTAGTATGG CTGAAGGCAT CAAAGTGACA GATGCTCCAA CCTATACAAC AAGAGATGAA 
CTGGTTGCCA AGAAGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304272.1
CDS	163..543
Translation	MAQEVSEYLSQNPRVAAWVEALRCDGETDKHWRHRRDFLLRNAGDLAPAGGAASASTDEAADAESGTRNRQLQQLISFSMAWANHVFLGCRYPQKVMDKILSMAEGIKVTDAPTYTTRDELVAKKG

Target ORF information:

RefSeq Version	NM_001317343.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CDKN2A interacting protein (CDKN2AIP), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317343.2

ORF Insert Sequence:

ATGGCGCAGG AGGTGTCGGA GTACCTGAGC CAGAACCCGC GGGTGGCAGC CTGGGTGGAG 
GCGCTGCGCT GCGACGGCGA GACTGACAAA CACTGGCGCC ACCGCCGGGA TTTTTTGCTT 
CGCAACGCCG GGGACCTGGC CCCCGCTGGC GGCGCTGCCT CCGCTAGCAC GGATGAAGCT 
GCCGACGCCG AGAGCGGGAC CCGAAACCGG CAGCTGCAGC AGCTCATCTC CTTTTCCATG 
GCCTGGGCGA ACCACGTCTT CCTCGGGTGC CGATACCCTC AAAAAGTTAT GGATAAAATA 
CTTAGTATGG CTGAAGGCAT CAAAGTGACA GATGCTCCAA CCTATACAAC AAGAGATGAA 
CTGGTTGCCA AGAAGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDKN2AIP ( NM_001317343.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304272.1 Homo sapiens CDKN2A interacting protein (CDKN2AIP), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101496
Clone ID Related Accession (Same CDS sequence)	NM_001317343.2 , NM_001317343.1
Accession Version	NM_001317343.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	CDKN2A-interacting protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN427056.1, AK096180.1, AK000043.1, AF246705.1, AC112698.4 and AA622329.1. Transcript Variant: This variant (2) uses an alternate splice junction at the 3' end of an exon compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.261577.1, SRR1803617.202206.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCAGG AGGTGTCGGA GTACCTGAGC CAGAACCCGC GGGTGGCAGC CTGGGTGGAG 
GCGCTGCGCT GCGACGGCGA GACTGACAAA CACTGGCGCC ACCGCCGGGA TTTTTTGCTT 
CGCAACGCCG GGGACCTGGC CCCCGCTGGC GGCGCTGCCT CCGCTAGCAC GGATGAAGCT 
GCCGACGCCG AGAGCGGGAC CCGAAACCGG CAGCTGCAGC AGCTCATCTC CTTTTCCATG 
GCCTGGGCGA ACCACGTCTT CCTCGGGTGC CGATACCCTC AAAAAGTTAT GGATAAAATA 
CTTAGTATGG CTGAAGGCAT CAAAGTGACA GATGCTCCAA CCTATACAAC AAGAGATGAA 
CTGGTTGCCA AGAAGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304272.1
CDS	208..588
Translation	MAQEVSEYLSQNPRVAAWVEALRCDGETDKHWRHRRDFLLRNAGDLAPAGGAASASTDEAADAESGTRNRQLQQLISFSMAWANHVFLGCRYPQKVMDKILSMAEGIKVTDAPTYTTRDELVAKKG

Target ORF information:

RefSeq Version	NM_001317343.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CDKN2A interacting protein (CDKN2AIP), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317343.1

ORF Insert Sequence:

ATGGCGCAGG AGGTGTCGGA GTACCTGAGC CAGAACCCGC GGGTGGCAGC CTGGGTGGAG 
GCGCTGCGCT GCGACGGCGA GACTGACAAA CACTGGCGCC ACCGCCGGGA TTTTTTGCTT 
CGCAACGCCG GGGACCTGGC CCCCGCTGGC GGCGCTGCCT CCGCTAGCAC GGATGAAGCT 
GCCGACGCCG AGAGCGGGAC CCGAAACCGG CAGCTGCAGC AGCTCATCTC CTTTTCCATG 
GCCTGGGCGA ACCACGTCTT CCTCGGGTGC CGATACCCTC AAAAAGTTAT GGATAAAATA 
CTTAGTATGG CTGAAGGCAT CAAAGTGACA GATGCTCCAA CCTATACAAC AAGAGATGAA 
CTGGTTGCCA AGAAGGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CARF is a multi-module regulator of cell proliferation and a molecular bridge between cellular senescence and carcinogenesis.
Mechanisms of ageing and development16664-68(2017 Sep)
Wadhwa R,Kalra RS,Kaul SC

CARF activates beta-catenin/TCF signaling in the hepatocellular carcinoma.
Oncotarget7(49)80404-80414(2016 Dec)
Fan X,Ma X,Cui L,Dang S,Qu J,Zhang J,Wang X,Mao Z

Loss-of-function screening to identify miRNAs involved in senescence: tumor suppressor activity of miRNA-335 and its new target CARF.
Scientific reports630185(2016 Jul)
Yu Y,Gao R,Kaul Z,Li L,Kato Y,Zhang Z,Groden J,Kaul SC,Wadhwa R

Collaborator of alternative reading frame protein (CARF) regulates early processing of pre-ribosomal RNA by retaining XRN2 (5'-3' exoribonuclease) in the nucleoplasm.
Nucleic acids research43(21)10397-410(2015 Dec)
Sato S,Ishikawa H,Yoshikawa H,Izumikawa K,Simpson RJ,Takahashi N

CARF (Collaborator of ARF) overexpression in p53-deficient cells promotes carcinogenesis.
Molecular oncology9(9)1877-89(2015 Nov)
Kalra RS,Cheung CT,Chaudhary A,Prakash J,Kaul SC,Wadhwa R