PPP3CA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP3CA
Entrez Gene ID	5530
Full Name	protein phosphatase 3 catalytic subunit alpha
Synonyms	CALN,CALNA,CALNA1,CCN1,CNA1,IECEE,PPP2B
General protein information	Preferred Names protein phosphatase 3 catalytic subunit alpha Names serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform CAM-PRP catalytic subunit calcineurin A alpha calmodulin-dependent calcineurin A subunit alpha isoform protein phosphatase 2B, catalytic subunit, alpha isoform protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform protein phosphatase 3 catalytic subunit alpha isozyme
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q24
Disorder MIM:	114105
Disorder Html:	Epileptic encephalopathy, infantile or early childhood, 617711 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000944.4	NP_000935.1	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 1
NM_001130692.1	NP_001124164.1	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 3
XM_024454127.1	XP_024309895.1	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform X2
XM_017008365.1	XP_016863854.1	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform X1
NM_001130691.2	NP_001124163.1	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 2
NM_001130691.1	NP_001124163.1	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 2
NM_000944.5	NP_000935.1	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 1
NM_001130692.2	NP_001124164.1	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPP3CA	NP_001184025.1
Rattus norvegicus (Norway rat)	Ppp3ca	NP_058737.1
Danio rerio (zebrafish)	ppp3cb	NP_001017856.1
Saccharomyces cerevisiae (baker's yeast)	CNA1	NP_013537.1
Neurospora crassa	NCU03804	XP_961193.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001279	XP_321872.5
Kluyveromyces lactis	KLLA0B05489g	XP_451780.1
Eremothecium gossypii	AGOS_AER118C	NP_984978.1
Magnaporthe oryzae (rice blast fungus)	MGG_07456	XP_003711354.1
Bos taurus (cattle)	PPP3CA	NP_777212.1
Danio rerio (zebrafish)	ppp3ca	NP_001185479.1
Schizosaccharomyces pombe (fission yeast)	ppb1	NP_596178.1
Xenopus tropicalis (tropical clawed frog)	ppp3ca	NP_001119980.1
Pan troglodytes (chimpanzee)	PPP3CA	XP_001168163.1
Mus musculus (house mouse)	Ppp3ca	NP_032939.1
Drosophila melanogaster (fruit fly)	CanA-14F	NP_001259622.1
Homo sapiens (human)	PPP3CA	NP_000935.1
	PPP3CA	XP_001108659.1
Gallus gallus (chicken)	PPP3CA	XP_420664.4
Drosophila melanogaster (fruit fly)	Pp2B-14D	NP_523373.2
Caenorhabditis elegans (roundworm)	tax-6	NP_001076658.2

Gene Ontology
Bibliography

Related articles in PubMed

Intrinsic disorder within AKAP79 fine-tunes anchored phosphatase activity toward substrates and drug sensitivity.
Nygren PJ, Mehta S, Schweppe DK, Langeberg LK, Whiting JL, Weisbrod CR, Bruce JE, Zhang J, Veesler D, Scott JD
eLife6(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identify a distinct C-terminal autoinhibitory four-residue sequence in CNAbeta1, (462)LAVP(465), which competitively inhibits substrate dephosphorylation. In vitro and cell-based assays revealed that the CNAbeta1-containing holoenzyme, CNbeta1, is autoinhibited at a single site by either of two inhibitory regions, CBD and LAVP, which block substrate access to the substrate-binding groove.
Title: The unique C terminus of the calcineurin isoform CNA?1 confers non-canonical regulation of enzyme activity by Ca

Mutation in PPP3CA gene is associated with Severe Neurodevelopmental Disease with Seizures.
Title: De Novo Mutations in PPP3CA Cause Severe Neurodevelopmental Disease with Seizures.

Studies indicate the importance of the AKAP79/PP2B/protein kinase A complex's role in synaptic long-term depression in the CA1 region of the hippocampus.
Title: Regulation of the phosphatase PP2B by protein-protein interactions.

Ca(2+)/calcineurin (CaN)/nuclear factor of activated T-cells (NFAT) c4 axis is required for neuritin-induced Kv4.2 transcriptional expression and potentiation of IA densities in cerebellum granule neurons.
Title: Neuritin Up-regulates Kv4.2 ?-Subunit of Potassium Channel Expression and Affects Neuronal Excitability by Regulating the Calcium-Calcineurin-NFATc4 Signaling Pathway.

CN-A and nephrin were clearly reduced in the kidneys of a 10-year-old boy who had relapsing steroid-resistant nephrotic syndrome.
Title: Role of calcineurin (CN) in kidney glomerular podocyte: CN inhibitor ameliorated proteinuria by inhibiting the redistribution of CN at the slit diaphragm.

The following PPP3CA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP3CA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13564	NM_000944.5 Latest version!	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$214.50-$300.30 $429.00
OHu13653	NM_001130691.2 Latest version!	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu13630	NM_001130692.2 Latest version!	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$279.30 $399.00
OHu105556	XM_024454127.1 Latest version!	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu95278	XM_017008365.1 Latest version!	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu13653	NM_001130691.1	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu13564	NM_000944.4	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$214.50-$300.30 $429.00
OHu13630	NM_001130692.1	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$279.30 $399.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP3CA ( NM_000944.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000935.1 Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13564
Clone ID Related Accession (Same CDS sequence)	NM_000944.4 , NM_000944.5
Accession Version	NM_000944.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-03
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EU192652.1, AL353950.1 and AW973358.1. On Jul 30, 2008 this sequence version replaced NM_000944.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL353950.1, SRR1660807.112431.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTTCCATTTC CTCCAAGTCA CCGGCTTACA GCAAAAGAAG TGTTTGATAA TGATGGAAAA 
CCTCGTGTGG ATATCTTAAA GGCGCATCTT ATGAAGGAGG GAAGGCTGGA AGAGAGTGTT 
GCATTGAGAA TAATAACAGA GGGTGCATCA ATTCTTCGAC AGGAAAAAAA TTTGCTGGAT 
ATTGATGCGC CAGTCACTGT TTGTGGGGAC ATTCATGGAC AATTCTTTGA TTTGATGAAG 
CTCTTTGAAG TCGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT TGAATGTGTG CTGTATTTGT GGGCCTTGAA AATTCTCTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGTATGGAT 
GCCTTTGACT GCCTTCCCCT GGCTGCCCTG ATGAACCAAC AGTTCCTGTG TGTGCATGGT 
GGTTTGTCTC CAGAGATTAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCAT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCCCTGGA AGATTTTGGA 
AATGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTACAG CACAATAACT TGTTATCTAT ACTCCGAGCC 
CACGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCT 
TCTCTAATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 
GTATTGAAGT ATGAGAACAA TGTTATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 
TACTGGCTTC CAAATTTCAT GGATGTTTTT ACTTGGTCCC TTCCATTTGT TGGGGAAAAA 
GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATCTGCTCAG ATGATGAACT AGGGTCAGAA 
GAAGATGGAT TTGATGGTGC AACAGCTGCA GCCCGGAAAG AGGTGATAAG GAACAAGATC 
CGAGCAATAG GCAAAATGGC CAGAGTGTTC TCAGTGCTCA GAGAAGAGAG TGAGAGTGTG 
CTGACGCTGA AAGGCTTGAC CCCAACTGGC ATGCTCCCCA GCGGAGTACT TTCTGGAGGG 
AAGCAAACCC TGCAAAGCGC TACTGTTGAG GCTATTGAGG CTGATGAAGC TATCAAAGGA 
TTTTCACCAC AACATAAGAT CACTAGCTTC GAGGAAGCCA AGGGCTTAGA CCGAATTAAT 
GAGAGGATGC CGCCTCGCAG AGATGCCATG CCCTCTGACG CCAACCTTAA CTCCATCAAC 
AAGGCTCTCA CCTCAGAGAC TAACGGCACG GACAGCAATG GCAGTAATAG CAGCAATATT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000935.1
CDS	676..2241
Translation	MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ

Target ORF information:

RefSeq Version	NM_000944.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000944.4

ORF Insert Sequence:

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTTCCATTTC CTCCAAGTCA CCGGCTTACA GCAAAAGAAG TGTTTGATAA TGATGGAAAA 
CCTCGTGTGG ATATCTTAAA GGCGCATCTT ATGAAGGAGG GAAGGCTGGA AGAGAGTGTT 
GCATTGAGAA TAATAACAGA GGGTGCATCA ATTCTTCGAC AGGAAAAAAA TTTGCTGGAT 
ATTGATGCGC CAGTCACTGT TTGTGGGGAC ATTCATGGAC AATTCTTTGA TTTGATGAAG 
CTCTTTGAAG TCGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT TGAATGTGTG CTGTATTTGT GGGCCTTGAA AATTCTCTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGTATGGAT 
GCCTTTGACT GCCTTCCCCT GGCTGCCCTG ATGAACCAAC AGTTCCTGTG TGTGCATGGT 
GGTTTGTCTC CAGAGATTAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCAT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCCCTGGA AGATTTTGGA 
AATGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTACAG CACAATAACT TGTTATCTAT ACTCCGAGCC 
CACGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCT 
TCTCTAATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 
GTATTGAAGT ATGAGAACAA TGTTATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 
TACTGGCTTC CAAATTTCAT GGATGTTTTT ACTTGGTCCC TTCCATTTGT TGGGGAAAAA 
GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATCTGCTCAG ATGATGAACT AGGGTCAGAA 
GAAGATGGAT TTGATGGTGC AACAGCTGCA GCCCGGAAAG AGGTGATAAG GAACAAGATC 
CGAGCAATAG GCAAAATGGC CAGAGTGTTC TCAGTGCTCA GAGAAGAGAG TGAGAGTGTG 
CTGACGCTGA AAGGCTTGAC CCCAACTGGC ATGCTCCCCA GCGGAGTACT TTCTGGAGGG 
AAGCAAACCC TGCAAAGCGC TACTGTTGAG GCTATTGAGG CTGATGAAGC TATCAAAGGA 
TTTTCACCAC AACATAAGAT CACTAGCTTC GAGGAAGCCA AGGGCTTAGA CCGAATTAAT 
GAGAGGATGC CGCCTCGCAG AGATGCCATG CCCTCTGACG CCAACCTTAA CTCCATCAAC 
AAGGCTCTCA CCTCAGAGAC TAACGGCACG GACAGCAATG GCAGTAATAG CAGCAATATT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP3CA ( NM_000944.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000935.1 Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13564
Clone ID Related Accession (Same CDS sequence)	NM_000944.4 , NM_000944.5
Accession Version	NM_000944.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP001816.2, EU192652.1, AL353950.1, AW973358.1 and AP001870.2. On Nov 22, 2018 this sequence version replaced NM_000944.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.112431.1, AL353950.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000394854.8/ ENSP00000378323.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTTCCATTTC CTCCAAGTCA CCGGCTTACA GCAAAAGAAG TGTTTGATAA TGATGGAAAA 
CCTCGTGTGG ATATCTTAAA GGCGCATCTT ATGAAGGAGG GAAGGCTGGA AGAGAGTGTT 
GCATTGAGAA TAATAACAGA GGGTGCATCA ATTCTTCGAC AGGAAAAAAA TTTGCTGGAT 
ATTGATGCGC CAGTCACTGT TTGTGGGGAC ATTCATGGAC AATTCTTTGA TTTGATGAAG 
CTCTTTGAAG TCGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT TGAATGTGTG CTGTATTTGT GGGCCTTGAA AATTCTCTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGTATGGAT 
GCCTTTGACT GCCTTCCCCT GGCTGCCCTG ATGAACCAAC AGTTCCTGTG TGTGCATGGT 
GGTTTGTCTC CAGAGATTAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCAT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCCCTGGA AGATTTTGGA 
AATGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTACAG CACAATAACT TGTTATCTAT ACTCCGAGCC 
CACGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCT 
TCTCTAATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 
GTATTGAAGT ATGAGAACAA TGTTATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 
TACTGGCTTC CAAATTTCAT GGATGTTTTT ACTTGGTCCC TTCCATTTGT TGGGGAAAAA 
GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATCTGCTCAG ATGATGAACT AGGGTCAGAA 
GAAGATGGAT TTGATGGTGC AACAGCTGCA GCCCGGAAAG AGGTGATAAG GAACAAGATC 
CGAGCAATAG GCAAAATGGC CAGAGTGTTC TCAGTGCTCA GAGAAGAGAG TGAGAGTGTG 
CTGACGCTGA AAGGCTTGAC CCCAACTGGC ATGCTCCCCA GCGGAGTACT TTCTGGAGGG 
AAGCAAACCC TGCAAAGCGC TACTGTTGAG GCTATTGAGG CTGATGAAGC TATCAAAGGA 
TTTTCACCAC AACATAAGAT CACTAGCTTC GAGGAAGCCA AGGGCTTAGA CCGAATTAAT 
GAGAGGATGC CGCCTCGCAG AGATGCCATG CCCTCTGACG CCAACCTTAA CTCCATCAAC 
AAGGCTCTCA CCTCAGAGAC TAACGGCACG GACAGCAATG GCAGTAATAG CAGCAATATT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000935.1
CDS	731..2296
Translation	MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ

Target ORF information:

RefSeq Version	NM_000944.5
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000944.5

ORF Insert Sequence:

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTTCCATTTC CTCCAAGTCA CCGGCTTACA GCAAAAGAAG TGTTTGATAA TGATGGAAAA 
CCTCGTGTGG ATATCTTAAA GGCGCATCTT ATGAAGGAGG GAAGGCTGGA AGAGAGTGTT 
GCATTGAGAA TAATAACAGA GGGTGCATCA ATTCTTCGAC AGGAAAAAAA TTTGCTGGAT 
ATTGATGCGC CAGTCACTGT TTGTGGGGAC ATTCATGGAC AATTCTTTGA TTTGATGAAG 
CTCTTTGAAG TCGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT TGAATGTGTG CTGTATTTGT GGGCCTTGAA AATTCTCTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGTATGGAT 
GCCTTTGACT GCCTTCCCCT GGCTGCCCTG ATGAACCAAC AGTTCCTGTG TGTGCATGGT 
GGTTTGTCTC CAGAGATTAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCAT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCCCTGGA AGATTTTGGA 
AATGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTACAG CACAATAACT TGTTATCTAT ACTCCGAGCC 
CACGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCT 
TCTCTAATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 
GTATTGAAGT ATGAGAACAA TGTTATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 
TACTGGCTTC CAAATTTCAT GGATGTTTTT ACTTGGTCCC TTCCATTTGT TGGGGAAAAA 
GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATCTGCTCAG ATGATGAACT AGGGTCAGAA 
GAAGATGGAT TTGATGGTGC AACAGCTGCA GCCCGGAAAG AGGTGATAAG GAACAAGATC 
CGAGCAATAG GCAAAATGGC CAGAGTGTTC TCAGTGCTCA GAGAAGAGAG TGAGAGTGTG 
CTGACGCTGA AAGGCTTGAC CCCAACTGGC ATGCTCCCCA GCGGAGTACT TTCTGGAGGG 
AAGCAAACCC TGCAAAGCGC TACTGTTGAG GCTATTGAGG CTGATGAAGC TATCAAAGGA 
TTTTCACCAC AACATAAGAT CACTAGCTTC GAGGAAGCCA AGGGCTTAGA CCGAATTAAT 
GAGAGGATGC CGCCTCGCAG AGATGCCATG CCCTCTGACG CCAACCTTAA CTCCATCAAC 
AAGGCTCTCA CCTCAGAGAC TAACGGCACG GACAGCAATG GCAGTAATAG CAGCAATATT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP3CA ( NM_001130691.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124163.1 Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13653
Clone ID Related Accession (Same CDS sequence)	NM_001130691.2 , NM_001130691.1
Accession Version	NM_001130691.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP001816.2, EU192652.1, BC025714.1, AW973358.1 and AP001870.2. On May 31, 2019 this sequence version replaced NM_001130691.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1, and encodes a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC025714.1, SRR1803614.248568.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTTCCATTTC CTCCAAGTCA CCGGCTTACA GCAAAAGAAG TGTTTGATAA TGATGGAAAA 
CCTCGTGTGG ATATCTTAAA GGCGCATCTT ATGAAGGAGG GAAGGCTGGA AGAGAGTGTT 
GCATTGAGAA TAATAACAGA GGGTGCATCA ATTCTTCGAC AGGAAAAAAA TTTGCTGGAT 
ATTGATGCGC CAGTCACTGT TTGTGGGGAC ATTCATGGAC AATTCTTTGA TTTGATGAAG 
CTCTTTGAAG TCGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT TGAATGTGTG CTGTATTTGT GGGCCTTGAA AATTCTCTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGTATGGAT 
GCCTTTGACT GCCTTCCCCT GGCTGCCCTG ATGAACCAAC AGTTCCTGTG TGTGCATGGT 
GGTTTGTCTC CAGAGATTAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCAT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCCCTGGA AGATTTTGGA 
AATGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTACAG CACAATAACT TGTTATCTAT ACTCCGAGCC 
CACGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCT 
TCTCTAATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 
GTATTGAAGT ATGAGAACAA TGTTATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 
TACTGGCTTC CAAATTTCAT GGATGTTTTT ACTTGGTCCC TTCCATTTGT TGGGGAAAAA 
GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATCTGCTCAG ATGATGAACT AGGGTCAGAA 
GAAGATGGAT TTGATGGTGC AACAGCTGCA GCCCGGAAAG AGGTGATAAG GAACAAGATC 
CGAGCAATAG GCAAAATGGC CAGAGTGTTC TCAGTGCTCA GAGAAGAGAG TGAGAGTGTG 
CTGACGCTGA AAGGCTTGAC CCCAACTGGC ATGCTCCCCA GCGGAGTACT TTCTGGAGGG 
AAGCAAACCC TGCAAAGCGC TATCAAAGGA TTTTCACCAC AACATAAGAT CACTAGCTTC 
GAGGAAGCCA AGGGCTTAGA CCGAATTAAT GAGAGGATGC CGCCTCGCAG AGATGCCATG 
CCCTCTGACG CCAACCTTAA CTCCATCAAC AAGGCTCTCA CCTCAGAGAC TAACGGCACG 
GACAGCAATG GCAGTAATAG CAGCAATATT CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124163.1
CDS	731..2266
Translation	MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ

Target ORF information:

RefSeq Version	NM_001130691.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130691.2

ORF Insert Sequence:

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTTCCATTTC CTCCAAGTCA CCGGCTTACA GCAAAAGAAG TGTTTGATAA TGATGGAAAA 
CCTCGTGTGG ATATCTTAAA GGCGCATCTT ATGAAGGAGG GAAGGCTGGA AGAGAGTGTT 
GCATTGAGAA TAATAACAGA GGGTGCATCA ATTCTTCGAC AGGAAAAAAA TTTGCTGGAT 
ATTGATGCGC CAGTCACTGT TTGTGGGGAC ATTCATGGAC AATTCTTTGA TTTGATGAAG 
CTCTTTGAAG TCGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT TGAATGTGTG CTGTATTTGT GGGCCTTGAA AATTCTCTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGTATGGAT 
GCCTTTGACT GCCTTCCCCT GGCTGCCCTG ATGAACCAAC AGTTCCTGTG TGTGCATGGT 
GGTTTGTCTC CAGAGATTAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCAT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCCCTGGA AGATTTTGGA 
AATGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTACAG CACAATAACT TGTTATCTAT ACTCCGAGCC 
CACGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCT 
TCTCTAATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 
GTATTGAAGT ATGAGAACAA TGTTATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 
TACTGGCTTC CAAATTTCAT GGATGTTTTT ACTTGGTCCC TTCCATTTGT TGGGGAAAAA 
GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATCTGCTCAG ATGATGAACT AGGGTCAGAA 
GAAGATGGAT TTGATGGTGC AACAGCTGCA GCCCGGAAAG AGGTGATAAG GAACAAGATC 
CGAGCAATAG GCAAAATGGC CAGAGTGTTC TCAGTGCTCA GAGAAGAGAG TGAGAGTGTG 
CTGACGCTGA AAGGCTTGAC CCCAACTGGC ATGCTCCCCA GCGGAGTACT TTCTGGAGGG 
AAGCAAACCC TGCAAAGCGC TATCAAAGGA TTTTCACCAC AACATAAGAT CACTAGCTTC 
GAGGAAGCCA AGGGCTTAGA CCGAATTAAT GAGAGGATGC CGCCTCGCAG AGATGCCATG 
CCCTCTGACG CCAACCTTAA CTCCATCAAC AAGGCTCTCA CCTCAGAGAC TAACGGCACG 
GACAGCAATG GCAGTAATAG CAGCAATATT CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP3CA ( NM_001130691.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124163.1 Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13653
Clone ID Related Accession (Same CDS sequence)	NM_001130691.2 , NM_001130691.1
Accession Version	NM_001130691.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EU192652.1, BC025714.1 and AW973358.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1, and encodes a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC025714.1, SRR1163657.192219.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTTCCATTTC CTCCAAGTCA CCGGCTTACA GCAAAAGAAG TGTTTGATAA TGATGGAAAA 
CCTCGTGTGG ATATCTTAAA GGCGCATCTT ATGAAGGAGG GAAGGCTGGA AGAGAGTGTT 
GCATTGAGAA TAATAACAGA GGGTGCATCA ATTCTTCGAC AGGAAAAAAA TTTGCTGGAT 
ATTGATGCGC CAGTCACTGT TTGTGGGGAC ATTCATGGAC AATTCTTTGA TTTGATGAAG 
CTCTTTGAAG TCGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT TGAATGTGTG CTGTATTTGT GGGCCTTGAA AATTCTCTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGTATGGAT 
GCCTTTGACT GCCTTCCCCT GGCTGCCCTG ATGAACCAAC AGTTCCTGTG TGTGCATGGT 
GGTTTGTCTC CAGAGATTAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCAT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCCCTGGA AGATTTTGGA 
AATGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTACAG CACAATAACT TGTTATCTAT ACTCCGAGCC 
CACGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCT 
TCTCTAATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 
GTATTGAAGT ATGAGAACAA TGTTATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 
TACTGGCTTC CAAATTTCAT GGATGTTTTT ACTTGGTCCC TTCCATTTGT TGGGGAAAAA 
GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATCTGCTCAG ATGATGAACT AGGGTCAGAA 
GAAGATGGAT TTGATGGTGC AACAGCTGCA GCCCGGAAAG AGGTGATAAG GAACAAGATC 
CGAGCAATAG GCAAAATGGC CAGAGTGTTC TCAGTGCTCA GAGAAGAGAG TGAGAGTGTG 
CTGACGCTGA AAGGCTTGAC CCCAACTGGC ATGCTCCCCA GCGGAGTACT TTCTGGAGGG 
AAGCAAACCC TGCAAAGCGC TATCAAAGGA TTTTCACCAC AACATAAGAT CACTAGCTTC 
GAGGAAGCCA AGGGCTTAGA CCGAATTAAT GAGAGGATGC CGCCTCGCAG AGATGCCATG 
CCCTCTGACG CCAACCTTAA CTCCATCAAC AAGGCTCTCA CCTCAGAGAC TAACGGCACG 
GACAGCAATG GCAGTAATAG CAGCAATATT CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124163.1
CDS	676..2211
Translation	MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ

Target ORF information:

RefSeq Version	NM_001130691.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130691.1

ORF Insert Sequence:

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTTCCATTTC CTCCAAGTCA CCGGCTTACA GCAAAAGAAG TGTTTGATAA TGATGGAAAA 
CCTCGTGTGG ATATCTTAAA GGCGCATCTT ATGAAGGAGG GAAGGCTGGA AGAGAGTGTT 
GCATTGAGAA TAATAACAGA GGGTGCATCA ATTCTTCGAC AGGAAAAAAA TTTGCTGGAT 
ATTGATGCGC CAGTCACTGT TTGTGGGGAC ATTCATGGAC AATTCTTTGA TTTGATGAAG 
CTCTTTGAAG TCGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT TGAATGTGTG CTGTATTTGT GGGCCTTGAA AATTCTCTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGTATGGAT 
GCCTTTGACT GCCTTCCCCT GGCTGCCCTG ATGAACCAAC AGTTCCTGTG TGTGCATGGT 
GGTTTGTCTC CAGAGATTAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCAT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCCCTGGA AGATTTTGGA 
AATGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTACAG CACAATAACT TGTTATCTAT ACTCCGAGCC 
CACGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCT 
TCTCTAATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 
GTATTGAAGT ATGAGAACAA TGTTATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 
TACTGGCTTC CAAATTTCAT GGATGTTTTT ACTTGGTCCC TTCCATTTGT TGGGGAAAAA 
GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATCTGCTCAG ATGATGAACT AGGGTCAGAA 
GAAGATGGAT TTGATGGTGC AACAGCTGCA GCCCGGAAAG AGGTGATAAG GAACAAGATC 
CGAGCAATAG GCAAAATGGC CAGAGTGTTC TCAGTGCTCA GAGAAGAGAG TGAGAGTGTG 
CTGACGCTGA AAGGCTTGAC CCCAACTGGC ATGCTCCCCA GCGGAGTACT TTCTGGAGGG 
AAGCAAACCC TGCAAAGCGC TATCAAAGGA TTTTCACCAC AACATAAGAT CACTAGCTTC 
GAGGAAGCCA AGGGCTTAGA CCGAATTAAT GAGAGGATGC CGCCTCGCAG AGATGCCATG 
CCCTCTGACG CCAACCTTAA CTCCATCAAC AAGGCTCTCA CCTCAGAGAC TAACGGCACG 
GACAGCAATG GCAGTAATAG CAGCAATATT CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP3CA ( NM_001130692.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124164.1 Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu13630
Clone ID Related Accession (Same CDS sequence)	NM_001130692.1 , NM_001130692.2
Accession Version	NM_001130692.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EU192652.1 and AW973358.1. Transcript Variant: This variant (3) lacks multiple in-frame exons in the 3' coding region, compared to variant 1, and encodes a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU192652.1, CD014046.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTTCCATTTC CTCCAAGTCA CCGGCTTACA GCAAAAGAAG TGTTTGATAA TGATGGAAAA 
CCTCGTGTGG ATATCTTAAA GGCGCATCTT ATGAAGGAGG GAAGGCTGGA AGAGAGTGTT 
GCATTGAGAA TAATAACAGA GGGTGCATCA ATTCTTCGAC AGGAAAAAAA TTTGCTGGAT 
ATTGATGCGC CAGTCACTGT TTGTGGGGAC ATTCATGGAC AATTCTTTGA TTTGATGAAG 
CTCTTTGAAG TCGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT TGAATGTGTG CTGTATTTGT GGGCCTTGAA AATTCTCTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGTATGGAT 
GCCTTTGACT GCCTTCCCCT GGCTGCCCTG ATGAACCAAC AGTTCCTGTG TGTGCATGGT 
GGTTTGTCTC CAGAGATTAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCAT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCCCTGGA AGATTTTGGA 
AATGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTACAG CACAATAACT TGTTATCTAT ACTCCGAGCC 
CACGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCT 
TCTCTAATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGTGACT 
GAGATGCTGG TAAATGTCCT CAACATCTGC TCAGATGATG AACTAGGGTC AGAAGAAGAT 
GGATTTGATG GTGCAACAGC TGCAGCCCGG AAAGAGGTGA TAAGGAACAA GATCCGAGCA 
ATAGGCAAAA TGGCCAGAGT GTTCTCAGTG CTCAGAGAAG AGAGTGAGAG TGTGCTGACG 
CTGAAAGGCT TGACCCCAAC TGGCATGCTC CCCAGCGGAG TACTTTCTGG AGGGAAGCAA 
ACCCTGCAAA GCGCTATCAA AGGATTTTCA CCACAACATA AGATCACTAG CTTCGAGGAA 
GCCAAGGGCT TAGACCGAAT TAATGAGAGG ATGCCGCCTC GCAGAGATGC CATGCCCTCT 
GACGCCAACC TTAACTCCAT CAACAAGGCT CTCACCTCAG AGACTAACGG CACGGACAGC 
AATGGCAGTA ATAGCAGCAA TATTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124164.1
CDS	676..2085
Translation	MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ

Target ORF information:

RefSeq Version	NM_001130692.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130692.1

ORF Insert Sequence:

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTTCCATTTC CTCCAAGTCA CCGGCTTACA GCAAAAGAAG TGTTTGATAA TGATGGAAAA 
CCTCGTGTGG ATATCTTAAA GGCGCATCTT ATGAAGGAGG GAAGGCTGGA AGAGAGTGTT 
GCATTGAGAA TAATAACAGA GGGTGCATCA ATTCTTCGAC AGGAAAAAAA TTTGCTGGAT 
ATTGATGCGC CAGTCACTGT TTGTGGGGAC ATTCATGGAC AATTCTTTGA TTTGATGAAG 
CTCTTTGAAG TCGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT TGAATGTGTG CTGTATTTGT GGGCCTTGAA AATTCTCTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGTATGGAT 
GCCTTTGACT GCCTTCCCCT GGCTGCCCTG ATGAACCAAC AGTTCCTGTG TGTGCATGGT 
GGTTTGTCTC CAGAGATTAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCAT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCCCTGGA AGATTTTGGA 
AATGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTACAG CACAATAACT TGTTATCTAT ACTCCGAGCC 
CACGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCT 
TCTCTAATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGTGACT 
GAGATGCTGG TAAATGTCCT CAACATCTGC TCAGATGATG AACTAGGGTC AGAAGAAGAT 
GGATTTGATG GTGCAACAGC TGCAGCCCGG AAAGAGGTGA TAAGGAACAA GATCCGAGCA 
ATAGGCAAAA TGGCCAGAGT GTTCTCAGTG CTCAGAGAAG AGAGTGAGAG TGTGCTGACG 
CTGAAAGGCT TGACCCCAAC TGGCATGCTC CCCAGCGGAG TACTTTCTGG AGGGAAGCAA 
ACCCTGCAAA GCGCTATCAA AGGATTTTCA CCACAACATA AGATCACTAG CTTCGAGGAA 
GCCAAGGGCT TAGACCGAAT TAATGAGAGG ATGCCGCCTC GCAGAGATGC CATGCCCTCT 
GACGCCAACC TTAACTCCAT CAACAAGGCT CTCACCTCAG AGACTAACGG CACGGACAGC 
AATGGCAGTA ATAGCAGCAA TATTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP3CA ( NM_001130692.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124164.1 Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu13630
Clone ID Related Accession (Same CDS sequence)	NM_001130692.1 , NM_001130692.2
Accession Version	NM_001130692.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP001816.2, EU192652.1, AW973358.1 and AP001870.2. On May 31, 2019 this sequence version replaced NM_001130692.1. Transcript Variant: This variant (3) lacks multiple in-frame exons in the 3' coding region, compared to variant 1, and encodes a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU192652.1, CD014046.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTTCCATTTC CTCCAAGTCA CCGGCTTACA GCAAAAGAAG TGTTTGATAA TGATGGAAAA 
CCTCGTGTGG ATATCTTAAA GGCGCATCTT ATGAAGGAGG GAAGGCTGGA AGAGAGTGTT 
GCATTGAGAA TAATAACAGA GGGTGCATCA ATTCTTCGAC AGGAAAAAAA TTTGCTGGAT 
ATTGATGCGC CAGTCACTGT TTGTGGGGAC ATTCATGGAC AATTCTTTGA TTTGATGAAG 
CTCTTTGAAG TCGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT TGAATGTGTG CTGTATTTGT GGGCCTTGAA AATTCTCTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGTATGGAT 
GCCTTTGACT GCCTTCCCCT GGCTGCCCTG ATGAACCAAC AGTTCCTGTG TGTGCATGGT 
GGTTTGTCTC CAGAGATTAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCAT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCCCTGGA AGATTTTGGA 
AATGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTACAG CACAATAACT TGTTATCTAT ACTCCGAGCC 
CACGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCT 
TCTCTAATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGTGACT 
GAGATGCTGG TAAATGTCCT CAACATCTGC TCAGATGATG AACTAGGGTC AGAAGAAGAT 
GGATTTGATG GTGCAACAGC TGCAGCCCGG AAAGAGGTGA TAAGGAACAA GATCCGAGCA 
ATAGGCAAAA TGGCCAGAGT GTTCTCAGTG CTCAGAGAAG AGAGTGAGAG TGTGCTGACG 
CTGAAAGGCT TGACCCCAAC TGGCATGCTC CCCAGCGGAG TACTTTCTGG AGGGAAGCAA 
ACCCTGCAAA GCGCTATCAA AGGATTTTCA CCACAACATA AGATCACTAG CTTCGAGGAA 
GCCAAGGGCT TAGACCGAAT TAATGAGAGG ATGCCGCCTC GCAGAGATGC CATGCCCTCT 
GACGCCAACC TTAACTCCAT CAACAAGGCT CTCACCTCAG AGACTAACGG CACGGACAGC 
AATGGCAGTA ATAGCAGCAA TATTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124164.1
CDS	731..2140
Translation	MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ

Target ORF information:

RefSeq Version	NM_001130692.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130692.2

ORF Insert Sequence:

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTTCCATTTC CTCCAAGTCA CCGGCTTACA GCAAAAGAAG TGTTTGATAA TGATGGAAAA 
CCTCGTGTGG ATATCTTAAA GGCGCATCTT ATGAAGGAGG GAAGGCTGGA AGAGAGTGTT 
GCATTGAGAA TAATAACAGA GGGTGCATCA ATTCTTCGAC AGGAAAAAAA TTTGCTGGAT 
ATTGATGCGC CAGTCACTGT TTGTGGGGAC ATTCATGGAC AATTCTTTGA TTTGATGAAG 
CTCTTTGAAG TCGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT TGAATGTGTG CTGTATTTGT GGGCCTTGAA AATTCTCTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGTATGGAT 
GCCTTTGACT GCCTTCCCCT GGCTGCCCTG ATGAACCAAC AGTTCCTGTG TGTGCATGGT 
GGTTTGTCTC CAGAGATTAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCAT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCCCTGGA AGATTTTGGA 
AATGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTACAG CACAATAACT TGTTATCTAT ACTCCGAGCC 
CACGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCT 
TCTCTAATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGTGACT 
GAGATGCTGG TAAATGTCCT CAACATCTGC TCAGATGATG AACTAGGGTC AGAAGAAGAT 
GGATTTGATG GTGCAACAGC TGCAGCCCGG AAAGAGGTGA TAAGGAACAA GATCCGAGCA 
ATAGGCAAAA TGGCCAGAGT GTTCTCAGTG CTCAGAGAAG AGAGTGAGAG TGTGCTGACG 
CTGAAAGGCT TGACCCCAAC TGGCATGCTC CCCAGCGGAG TACTTTCTGG AGGGAAGCAA 
ACCCTGCAAA GCGCTATCAA AGGATTTTCA CCACAACATA AGATCACTAG CTTCGAGGAA 
GCCAAGGGCT TAGACCGAAT TAATGAGAGG ATGCCGCCTC GCAGAGATGC CATGCCCTCT 
GACGCCAACC TTAACTCCAT CAACAAGGCT CTCACCTCAG AGACTAACGG CACGGACAGC 
AATGGCAGTA ATAGCAGCAA TATTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP3CA ( XM_024454127.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309895.1 Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant X2, mRNA.

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CloneID	OHu105556
Clone ID Related Accession (Same CDS sequence)	XM_024454127.1
Accession Version	XM_024454127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGG GAAGGCTGGA AGAGAGTGTT GCATTGAGAA TAATAACAGA GGGTGCATCA 
ATTCTTCGAC AGGAAAAAAA TTTGCTGGAT ATTGATGCGC CAGTCACTGT TTGTGGGGAC 
ATTCATGGAC AATTCTTTGA TTTGATGAAG CTCTTTGAAG TCGGGGGATC TCCTGCCAAC 
ACTCGCTACC TCTTCTTAGG GGACTATGTT GACAGAGGGT ACTTCAGTAT TGAATGTGTG 
CTGTATTTGT GGGCCTTGAA AATTCTCTAC CCCAAAACAC TGTTTTTACT TCGTGGAAAT 
CATGAATGTA GACATCTAAC AGAGTATTTC ACATTTAAAC AAGAATGTAA AATAAAGTAT 
TCAGAACGCG TATATGATGC CTGTATGGAT GCCTTTGACT GCCTTCCCCT GGCTGCCCTG 
ATGAACCAAC AGTTCCTGTG TGTGCATGGT GGTTTGTCTC CAGAGATTAA CACTTTAGAT 
GATATCAGAA AATTAGACCG ATTCAAAGAA CCACCTGCAT ATGGACCTAT GTGTGATATC 
CTGTGGTCAG ACCCCCTGGA AGATTTTGGA AATGAGAAGA CTCAGGAACA TTTCACTCAC 
AACACAGTCA GGGGGTGTTC ATACTTCTAC AGTTACCCGG CTGTATGTGA ATTCTTACAG 
CACAATAACT TGTTATCTAT ACTCCGAGCC CACGAAGCCC AAGATGCAGG GTACCGCATG 
TACAGGAAAA GCCAAACAAC AGGCTTCCCT TCTCTAATTA CAATTTTTTC AGCACCAAAT 
TACTTAGATG TATACAATAA CAAAGCTGCA GTATTGAAGT ATGAGAACAA TGTTATGAAT 
ATCAGGCAAT TCAACTGTTC TCCTCATCCA TACTGGCTTC CAAATTTCAT GGATGTTTTT 
ACTTGGTCCC TTCCATTTGT TGGGGAAAAA GTGACTGAGA TGCTGGTAAA TGTCCTCAAC 
ATCTGCTCAG ATGATGAACT AGGGTCAGAA GAAGATGGAT TTGATGGTGC AACAGCTGCA 
GCCCGGAAAG AGGTGATAAG GAACAAGATC CGAGCAATAG GCAAAATGGC CAGAGTGTTC 
TCAGTGCTCA GAGAAGAGAG TGAGAGTGTG CTGACGCTGA AAGGCTTGAC CCCAACTGGC 
ATGCTCCCCA GCGGAGTACT TTCTGGAGGG AAGCAAACCC TGCAAAGCGC TACTGTTGAG 
GCTATTGAGG CTGATGAAGC TATCAAAGGA TTTTCACCAC AACATAAGAT CACTAGCTTC 
GAGGAAGCCA AGGGCTTAGA CCGAATTAAT GAGAGGATGC CGCCTCGCAG AGATGCCATG 
CCCTCTGACG CCAACCTTAA CTCCATCAAC AAGGCTCTCA CCTCAGAGAC TAACGGCACG 
GACAGCAATG GCAGTAATAG CAGCAATATT CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309895.1
CDS	156..1571
Translation	MKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ

Target ORF information:

RefSeq Version	XM_024454127.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454127.1

ORF Insert Sequence:

ATGAAGGAGG GAAGGCTGGA AGAGAGTGTT GCATTGAGAA TAATAACAGA GGGTGCATCA 
ATTCTTCGAC AGGAAAAAAA TTTGCTGGAT ATTGATGCGC CAGTCACTGT TTGTGGGGAC 
ATTCATGGAC AATTCTTTGA TTTGATGAAG CTCTTTGAAG TCGGGGGATC TCCTGCCAAC 
ACTCGCTACC TCTTCTTAGG GGACTATGTT GACAGAGGGT ACTTCAGTAT TGAATGTGTG 
CTGTATTTGT GGGCCTTGAA AATTCTCTAC CCCAAAACAC TGTTTTTACT TCGTGGAAAT 
CATGAATGTA GACATCTAAC AGAGTATTTC ACATTTAAAC AAGAATGTAA AATAAAGTAT 
TCAGAACGCG TATATGATGC CTGTATGGAT GCCTTTGACT GCCTTCCCCT GGCTGCCCTG 
ATGAACCAAC AGTTCCTGTG TGTGCATGGT GGTTTGTCTC CAGAGATTAA CACTTTAGAT 
GATATCAGAA AATTAGACCG ATTCAAAGAA CCACCTGCAT ATGGACCTAT GTGTGATATC 
CTGTGGTCAG ACCCCCTGGA AGATTTTGGA AATGAGAAGA CTCAGGAACA TTTCACTCAC 
AACACAGTCA GGGGGTGTTC ATACTTCTAC AGTTACCCGG CTGTATGTGA ATTCTTACAG 
CACAATAACT TGTTATCTAT ACTCCGAGCC CACGAAGCCC AAGATGCAGG GTACCGCATG 
TACAGGAAAA GCCAAACAAC AGGCTTCCCT TCTCTAATTA CAATTTTTTC AGCACCAAAT 
TACTTAGATG TATACAATAA CAAAGCTGCA GTATTGAAGT ATGAGAACAA TGTTATGAAT 
ATCAGGCAAT TCAACTGTTC TCCTCATCCA TACTGGCTTC CAAATTTCAT GGATGTTTTT 
ACTTGGTCCC TTCCATTTGT TGGGGAAAAA GTGACTGAGA TGCTGGTAAA TGTCCTCAAC 
ATCTGCTCAG ATGATGAACT AGGGTCAGAA GAAGATGGAT TTGATGGTGC AACAGCTGCA 
GCCCGGAAAG AGGTGATAAG GAACAAGATC CGAGCAATAG GCAAAATGGC CAGAGTGTTC 
TCAGTGCTCA GAGAAGAGAG TGAGAGTGTG CTGACGCTGA AAGGCTTGAC CCCAACTGGC 
ATGCTCCCCA GCGGAGTACT TTCTGGAGGG AAGCAAACCC TGCAAAGCGC TACTGTTGAG 
GCTATTGAGG CTGATGAAGC TATCAAAGGA TTTTCACCAC AACATAAGAT CACTAGCTTC 
GAGGAAGCCA AGGGCTTAGA CCGAATTAAT GAGAGGATGC CGCCTCGCAG AGATGCCATG 
CCCTCTGACG CCAACCTTAA CTCCATCAAC AAGGCTCTCA CCTCAGAGAC TAACGGCACG 
GACAGCAATG GCAGTAATAG CAGCAATATT CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP3CA ( XM_017008365.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863854.1 Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant X1, mRNA.

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CloneID	OHu95278
Clone ID Related Accession (Same CDS sequence)	XM_017008365.1
Accession Version	XM_017008365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGCAT CCATCTGTAC AGCTGTTCCA TTTCCTCCAA GTCACCGGCT TACAGCAAAA 
GAAGTGTTTG ATAATGATGG AAAACCTCGT GTGGATATCT TAAAGGCGCA TCTTATGAAG 
GAGGGAAGGC TGGAAGAGAG TGTTGCATTG AGAATAATAA CAGAGGGTGC ATCAATTCTT 
CGACAGGAAA AAAATTTGCT GGATATTGAT GCGCCAGTCA CTGTTTGTGG GGACATTCAT 
GGACAATTCT TTGATTTGAT GAAGCTCTTT GAAGTCGGGG GATCTCCTGC CAACACTCGC 
TACCTCTTCT TAGGGGACTA TGTTGACAGA GGGTACTTCA GTATTGAATG TGTGCTGTAT 
TTGTGGGCCT TGAAAATTCT CTACCCCAAA ACACTGTTTT TACTTCGTGG AAATCATGAA 
TGTAGACATC TAACAGAGTA TTTCACATTT AAACAAGAAT GTAAAATAAA GTATTCAGAA 
CGCGTATATG ATGCCTGTAT GGATGCCTTT GACTGCCTTC CCCTGGCTGC CCTGATGAAC 
CAACAGTTCC TGTGTGTGCA TGGTGGTTTG TCTCCAGAGA TTAACACTTT AGATGATATC 
AGAAAATTAG ACCGATTCAA AGAACCACCT GCATATGGAC CTATGTGTGA TATCCTGTGG 
TCAGACCCCC TGGAAGATTT TGGAAATGAG AAGACTCAGG AACATTTCAC TCACAACACA 
GTCAGGGGGT GTTCATACTT CTACAGTTAC CCGGCTGTAT GTGAATTCTT ACAGCACAAT 
AACTTGTTAT CTATACTCCG AGCCCACGAA GCCCAAGATG CAGGGTACCG CATGTACAGG 
AAAAGCCAAA CAACAGGCTT CCCTTCTCTA ATTACAATTT TTTCAGCACC AAATTACTTA 
GATGTATACA ATAACAAAGC TGCAGTATTG AAGTATGAGA ACAATGTTAT GAATATCAGG 
CAATTCAACT GTTCTCCTCA TCCATACTGG CTTCCAAATT TCATGGATGT TTTTACTTGG 
TCCCTTCCAT TTGTTGGGGA AAAAGTGACT GAGATGCTGG TAAATGTCCT CAACATCTGC 
TCAGATGATG AACTAGGGTC AGAAGAAGAT GGATTTGATG GTGCAACAGC TGCAGCCCGG 
AAAGAGGTGA TAAGGAACAA GATCCGAGCA ATAGGCAAAA TGGCCAGAGT GTTCTCAGTG 
CTCAGAGAAG AGAGTGAGAG TGTGCTGACG CTGAAAGGCT TGACCCCAAC TGGCATGCTC 
CCCAGCGGAG TACTTTCTGG AGGGAAGCAA ACCCTGCAAA GCGCTACTGT TGAGGCTATT 
GAGGCTGATG AAGCTATCAA AGGATTTTCA CCACAACATA AGATCACTAG CTTCGAGGAA 
GCCAAGGGCT TAGACCGAAT TAATGAGAGG ATGCCGCCTC GCAGAGATGC CATGCCCTCT 
GACGCCAACC TTAACTCCAT CAACAAGGCT CTCACCTCAG AGACTAACGG CACGGACAGC 
AATGGCAGTA ATAGCAGCAA TATTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863854.1
CDS	16..1545
Translation	MLASICTAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ

Target ORF information:

RefSeq Version	XM_017008365.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008365.1

ORF Insert Sequence:

ATGCTTGCAT CCATCTGTAC AGCTGTTCCA TTTCCTCCAA GTCACCGGCT TACAGCAAAA 
GAAGTGTTTG ATAATGATGG AAAACCTCGT GTGGATATCT TAAAGGCGCA TCTTATGAAG 
GAGGGAAGGC TGGAAGAGAG TGTTGCATTG AGAATAATAA CAGAGGGTGC ATCAATTCTT 
CGACAGGAAA AAAATTTGCT GGATATTGAT GCGCCAGTCA CTGTTTGTGG GGACATTCAT 
GGACAATTCT TTGATTTGAT GAAGCTCTTT GAAGTCGGGG GATCTCCTGC CAACACTCGC 
TACCTCTTCT TAGGGGACTA TGTTGACAGA GGGTACTTCA GTATTGAATG TGTGCTGTAT 
TTGTGGGCCT TGAAAATTCT CTACCCCAAA ACACTGTTTT TACTTCGTGG AAATCATGAA 
TGTAGACATC TAACAGAGTA TTTCACATTT AAACAAGAAT GTAAAATAAA GTATTCAGAA 
CGCGTATATG ATGCCTGTAT GGATGCCTTT GACTGCCTTC CCCTGGCTGC CCTGATGAAC 
CAACAGTTCC TGTGTGTGCA TGGTGGTTTG TCTCCAGAGA TTAACACTTT AGATGATATC 
AGAAAATTAG ACCGATTCAA AGAACCACCT GCATATGGAC CTATGTGTGA TATCCTGTGG 
TCAGACCCCC TGGAAGATTT TGGAAATGAG AAGACTCAGG AACATTTCAC TCACAACACA 
GTCAGGGGGT GTTCATACTT CTACAGTTAC CCGGCTGTAT GTGAATTCTT ACAGCACAAT 
AACTTGTTAT CTATACTCCG AGCCCACGAA GCCCAAGATG CAGGGTACCG CATGTACAGG 
AAAAGCCAAA CAACAGGCTT CCCTTCTCTA ATTACAATTT TTTCAGCACC AAATTACTTA 
GATGTATACA ATAACAAAGC TGCAGTATTG AAGTATGAGA ACAATGTTAT GAATATCAGG 
CAATTCAACT GTTCTCCTCA TCCATACTGG CTTCCAAATT TCATGGATGT TTTTACTTGG 
TCCCTTCCAT TTGTTGGGGA AAAAGTGACT GAGATGCTGG TAAATGTCCT CAACATCTGC 
TCAGATGATG AACTAGGGTC AGAAGAAGAT GGATTTGATG GTGCAACAGC TGCAGCCCGG 
AAAGAGGTGA TAAGGAACAA GATCCGAGCA ATAGGCAAAA TGGCCAGAGT GTTCTCAGTG 
CTCAGAGAAG AGAGTGAGAG TGTGCTGACG CTGAAAGGCT TGACCCCAAC TGGCATGCTC 
CCCAGCGGAG TACTTTCTGG AGGGAAGCAA ACCCTGCAAA GCGCTACTGT TGAGGCTATT 
GAGGCTGATG AAGCTATCAA AGGATTTTCA CCACAACATA AGATCACTAG CTTCGAGGAA 
GCCAAGGGCT TAGACCGAAT TAATGAGAGG ATGCCGCCTC GCAGAGATGC CATGCCCTCT 
GACGCCAACC TTAACTCCAT CAACAAGGCT CTCACCTCAG AGACTAACGG CACGGACAGC 
AATGGCAGTA ATAGCAGCAA TATTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Intrinsic disorder within AKAP79 fine-tunes anchored phosphatase activity toward substrates and drug sensitivity.
eLife6(2017 Oct)
Nygren PJ,Mehta S,Schweppe DK,Langeberg LK,Whiting JL,Weisbrod CR,Bruce JE,Zhang J,Veesler D,Scott JD

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PPP3CA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム