APPL2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	APPL2
Entrez Gene ID	55198
Full Name	adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2
Synonyms	DIP13B
General protein information	Preferred Names adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 Names DCC-interacting protein 13-beta DIP13 beta adapter protein containing PH domain, PTB domain and leucine zipper motif 2 adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q23.3
Summary	The protein encoded by this gene is one of two effectors of the small GTPase RAB5A/Rab5, which are involved in a signal transduction pathway. Both effectors contain an N-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain, and a C-terminal phosphotyrosine binding (PTB) domain, and they bind the Rab5 through the BAR domain. They are associated with endosomal membranes and can be translocated to the nucleus in response to the EGF stimulus. They interact with the NuRD/MeCP1 complex (nucleosome remodeling and deacetylase /methyl-CpG-binding protein 1 complex) and are required for efficient cell proliferation. A chromosomal aberration t(12;22)(q24.1;q13.3) involving this gene and the PSAP2 gene results in 22q13.3 deletion syndrome, also known as Phelan-McDermid syndrome. [provided by RefSeq, Oct 2011].
Disorder MIM:	606231

mRNA and Protein(s)

mRNA	Protein	Name
XM_011538530.2	XP_011536832.1	DCC-interacting protein 13-beta isoform X1
XM_017019553.2	XP_016875042.1	DCC-interacting protein 13-beta isoform X5
NM_018171.4	NP_060641.2	DCC-interacting protein 13-beta isoform 1
XM_017019553.1	XP_016875042.1	DCC-interacting protein 13-beta isoform X5
NM_018171.3	NP_060641.2	DCC-interacting protein 13-beta isoform 1
NM_018171.5	NP_060641.2	DCC-interacting protein 13-beta isoform 1
XM_017019551.2	XP_016875040.1	DCC-interacting protein 13-beta isoform X2
XM_011538531.3	XP_011536833.1	DCC-interacting protein 13-beta isoform X3
XM_006719472.1	XP_006719535.1	DCC-interacting protein 13-beta isoform X4
XM_017019554.1	XP_016875043.1	DCC-interacting protein 13-beta isoform X6
XM_017019551.1	XP_016875040.1	DCC-interacting protein 13-beta isoform X2
XM_011538531.2	XP_011536833.1	DCC-interacting protein 13-beta isoform X3
XM_011538532.2	XP_011536834.1	DCC-interacting protein 13-beta isoform X3
XM_011538530.3	XP_011536832.1	DCC-interacting protein 13-beta isoform X1
XM_017019552.2	XP_016875041.1	DCC-interacting protein 13-beta isoform X5
NM_001251904.1	NP_001238833.1	DCC-interacting protein 13-beta isoform 2
XM_017019552.1	XP_016875041.1	DCC-interacting protein 13-beta isoform X5
XM_011538532.3	XP_011536834.1	DCC-interacting protein 13-beta isoform X3
NM_001251905.1	NP_001238834.1	DCC-interacting protein 13-beta isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	APPL2	NP_060641.2
Danio rerio (zebrafish)	appl2	NP_001121547.1
Rattus norvegicus (Norway rat)	Appl2	NP_001102211.1
Gallus gallus (chicken)	LOC100857286	XP_003640457.1
	LOC701612	XP_002798807.1
Bos taurus (cattle)	APPL2	NP_001039671.1
Xenopus tropicalis (tropical clawed frog)	appl2	NP_001106389.1
Mus musculus (house mouse)	Appl2	NP_660255.1
Gallus gallus (chicken)	APPL2	XP_416311.2
Pan troglodytes (chimpanzee)	APPL2	XP_001160746.1
Canis lupus familiaris (dog)	APPL2	XP_538418.2

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Alternative splicing of the OCC-1 gene generates three splice variants and a novel exonic microRNA, which regulate the Wnt signaling pathway.
Najafi H, Soltani BM, Dokanehiifard S, Nasiri S, Mowla SJ
RNA (New York, N.Y.)23(1)70-85(2017 01)

An inter-species protein-protein interaction network across vast evolutionary distance.
Zhong Q, Pevzner SJ, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Ta?an M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barab?si AL, Hill DE, Aloy P, Cusick ME, Xia Y, Roth FP, Vidal M
Molecular systems biology12(4)865(2016 Apr)

APPL proteins promote TGF?-induced nuclear transport of the TGF? type I receptor intracellular domain.
Song J, Mu Y, Li C, Bergh A, Miaczynska M, Heldin CH, Landstr?m M
Oncotarget7(1)279-92(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show the suppressive effect of OCC-1 RNA on transcription level of the APPL2 gene provides a putative colorectal neoplasm progression index.
Title: Alternative splicing of the OCC-1 gene generates three splice variants and a novel exonic microRNA, which regulate the Wnt signaling pathway.

Data show that signal transducing adaptor proteins APPL1 and APPL2 are required for TGFbeta-induced nuclear translocation of TGFbeta type I receptor (TbetaRI)-ICD and for cancer cell invasiveness of prostate and breast cancer cell lines.
Title: APPL proteins promote TGF?-induced nuclear transport of the TGF? type I receptor intracellular domain.

ATM is the central modulator of APPL-mediated effects on radiosensitivity and DNA repair.
Title: APPL proteins modulate DNA repair and radiation survival of pancreatic carcinoma cells by regulating ATM.

It concludes that APPL2(PH) binding to BAR domain and Reptin is mutually exclusive which regulates the nucleocytoplasmic shuttling of Reptin.
Title: Mutually exclusive binding of APPL(PH) to BAR domain and Reptin regulates ?-catenin dependent transcriptional events.

C-APPL1/A-APPL2 allele combination is associated with non-alcoholic fatty liver disease occurrence, with a more severe hepatic steatosis grade and with a reduced adiponectin cytoprotective effect on liver.
Title: Association of genetic variation in adaptor protein APPL1/APPL2 loci with non-alcoholic fatty liver disease.

The following APPL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APPL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15669	NM_001251905.1 Latest version!	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu15669	XM_017019552.2 Latest version!	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu15669	XM_017019553.2 Latest version!	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu46658	XM_006719472.1 Latest version!	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu69147	XM_011538530.3 Latest version!	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu69148	XM_011538531.3 Latest version!	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu69148	XM_011538532.3 Latest version!	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu15838	NM_001251904.1 Latest version!	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu17777	NM_018171.5 Latest version!	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu83929	XM_017019551.2 Latest version!	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu83930	XM_017019554.1 Latest version!	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu15669	XM_017019553.1	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu17777	NM_018171.3	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu17777	NM_018171.4	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu83929	XM_017019551.1	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu69148	XM_011538531.2	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu69147	XM_011538530.2	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu15669	XM_017019552.1	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu69148	XM_011538532.2	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APPL2 ( NM_001251905.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001238834.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 3, mRNA.

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CloneID	OHu15669
Clone ID Related Accession (Same CDS sequence)	XM_017019553.2 , XM_017019553.1 , XM_017019552.2 , XM_017019552.1 , NM_001251905.1
Accession Version	NM_001251905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN993818.1, AK296610.1 and BC028008.1. Transcript Variant: This variant (3) differs in the 5' exon, which results in a downstream AUG start codon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.192530.1, SRR1803616.12025.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGAGCA TGACCTCTCA 
ATGGCAAAAT ACAGCAGGCT GCCTAAGAAA AAGGAGAATG AGAAGGTGAA GACCGAAGTC 
GGAAAAGAGG TGGCCGCGGC CCGGCGGAAG CAGCACCTCT CCTCCCTTCA GTACTACTGT 
GCCCTCAACG CGCTGCAGTA CAGAAAGCAA ATGGCCATGA TGGAGCCCAT GATAGGCTTT 
GCCCATGGAC AGATTAACTT TTTTAAGAAG GGAGCAGAGA TGTTTTCCAA ACGTATGGAC 
AGCTTTTTAT CCTCCGTTGC AGACATGGTT CAAAGCATTC AGGTAGAACT GGAAGCCGAG 
GCGGAAAAGA TGCGGGTGTC CCAGCAAGAA TTACTTTCTG TTGATGAATC TGTTTACACT 
CCAGACTCTG ATGTGGCCGC ACCACAGATC AACAGGAACC TCATCCAGAA GGCTGGTTAC 
CTTAATCTTA GAAACAAAAC AGGGCTGGTC ACCACCACCT GGGAGAGGCT TTATTTCTTC 
ACCCAAGGCG GGAATCTCAT GTGTCAGCCC AGGGGAGCCG TGGCTGGAGG TTTGATCCAG 
GACCTGGACA ACTGCTCAGT GATGGCCGTG GATTGCGAAG ACCGGCGCTA CTGCTTCCAG 
ATCACCACGC CCAATGGAAA ATCGGGAATA ATCCTCCAGG CTGAGAGCAG AAAGGAAAAT 
GAAGAGTGGA TATGTGCAAT AAACAACATC TCCAGACAGA TCTACCTGAC CGACAACCCT 
GAGGCAGTCG CGATCAAGTT GAATCAGACC GCTCTGCAAG CAGTGACTCC CATTACAAGT 
TTTGGAAAAA AACAAGAAAG CTCATGCCCC AGCCAGAACC TGAAAAATTC AGAGATGGAA 
AATGAAAATG ACAAGATTGT TCCCAAAGCA ACAGCCAGTC TACCTGAAGC AGAGGAGCTG 
ATCGCGCCTG GAACGCCGAT TCAATTCGAT ATTGTGCTTC CTGCTACAGA ATTCCTTGAT 
CAGAACAGAG GGAGCAGGCG TACCAACCCT TTTGGTGAAA CTGAGGATGA ATCATTTCCA 
GAAGCAGAAG ATTCTCTTTT GCAGCAGATG TTTATAGTTC GGTTTTTGGG ATCAATGGCA 
GTTAAAACAG ACAGCACTAC TGAAGTGATT TATGAAGCGA TGAGACAAGT ATTGGCTGCT 
CGGGCTATTC ATAACATCTT CCGCATGACA GAATCCCATC TGATGGTCAC CAGTCAATCT 
TTGAGGTTGA TAGATCCACA GACTCAAGTA TCAAGGGCCA ATTTTGAACT TACCAGTGTC 
ACACAATTTG CTGCTCATCA AGAAAACAAG AGACTGGTTG GTTTTGTCAT CCGTGTTCCT 
GAATCCACTG GAGAAGAATC TCTGAGTACA TACATTTTTG AAAGCAACTC AGAAGGCGAA 
AAGATATGTT ATGCTATTAA TTTGGGAAAA GAAATTATTG AGGTTCAGAA GGATCCAGAA 
GCACTGGCTC AATTAATGCT GTCCATACCA CTAACCAATG ACGGAAAATA TGTACTGTTA 
AACGATCAAC CAGATGACGA TGATGGAAAT CCAAACGAAC ATAGAGGCGC AGAATCCGAA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238834.1
CDS	391..2256
Translation	MQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	NM_001251905.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001251905.1

ORF Insert Sequence:

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGAGCA TGACCTCTCA 
ATGGCAAAAT ACAGCAGGCT GCCTAAGAAA AAGGAGAATG AGAAGGTGAA GACCGAAGTC 
GGAAAAGAGG TGGCCGCGGC CCGGCGGAAG CAGCACCTCT CCTCCCTTCA GTACTACTGT 
GCCCTCAACG CGCTGCAGTA CAGAAAGCAA ATGGCCATGA TGGAGCCCAT GATAGGCTTT 
GCCCATGGAC AGATTAACTT TTTTAAGAAG GGAGCAGAGA TGTTTTCCAA ACGTATGGAC 
AGCTTTTTAT CCTCCGTTGC AGACATGGTT CAAAGCATTC AGGTAGAACT GGAAGCCGAG 
GCGGAAAAGA TGCGGGTGTC CCAGCAAGAA TTACTTTCTG TTGATGAATC TGTTTACACT 
CCAGACTCTG ATGTGGCCGC ACCACAGATC AACAGGAACC TCATCCAGAA GGCTGGTTAC 
CTTAATCTTA GAAACAAAAC AGGGCTGGTC ACCACCACCT GGGAGAGGCT TTATTTCTTC 
ACCCAAGGCG GGAATCTCAT GTGTCAGCCC AGGGGAGCCG TGGCTGGAGG TTTGATCCAG 
GACCTGGACA ACTGCTCAGT GATGGCCGTG GATTGCGAAG ACCGGCGCTA CTGCTTCCAG 
ATCACCACGC CCAATGGAAA ATCGGGAATA ATCCTCCAGG CTGAGAGCAG AAAGGAAAAT 
GAAGAGTGGA TATGTGCAAT AAACAACATC TCCAGACAGA TCTACCTGAC CGACAACCCT 
GAGGCAGTCG CGATCAAGTT GAATCAGACC GCTCTGCAAG CAGTGACTCC CATTACAAGT 
TTTGGAAAAA AACAAGAAAG CTCATGCCCC AGCCAGAACC TGAAAAATTC AGAGATGGAA 
AATGAAAATG ACAAGATTGT TCCCAAAGCA ACAGCCAGTC TACCTGAAGC AGAGGAGCTG 
ATCGCGCCTG GAACGCCGAT TCAATTCGAT ATTGTGCTTC CTGCTACAGA ATTCCTTGAT 
CAGAACAGAG GGAGCAGGCG TACCAACCCT TTTGGTGAAA CTGAGGATGA ATCATTTCCA 
GAAGCAGAAG ATTCTCTTTT GCAGCAGATG TTTATAGTTC GGTTTTTGGG ATCAATGGCA 
GTTAAAACAG ACAGCACTAC TGAAGTGATT TATGAAGCGA TGAGACAAGT ATTGGCTGCT 
CGGGCTATTC ATAACATCTT CCGCATGACA GAATCCCATC TGATGGTCAC CAGTCAATCT 
TTGAGGTTGA TAGATCCACA GACTCAAGTA TCAAGGGCCA ATTTTGAACT TACCAGTGTC 
ACACAATTTG CTGCTCATCA AGAAAACAAG AGACTGGTTG GTTTTGTCAT CCGTGTTCCT 
GAATCCACTG GAGAAGAATC TCTGAGTACA TACATTTTTG AAAGCAACTC AGAAGGCGAA 
AAGATATGTT ATGCTATTAA TTTGGGAAAA GAAATTATTG AGGTTCAGAA GGATCCAGAA 
GCACTGGCTC AATTAATGCT GTCCATACCA CTAACCAATG ACGGAAAATA TGTACTGTTA 
AACGATCAAC CAGATGACGA TGATGGAAAT CCAAACGAAC ATAGAGGCGC AGAATCCGAA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_017019553.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875042.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu15669
Clone ID Related Accession (Same CDS sequence)	XM_017019553.2 , XM_017019553.1 , XM_017019552.2 , XM_017019552.1 , NM_001251905.1
Accession Version	XM_017019553.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017019553.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGAGCA TGACCTCTCA 
ATGGCAAAAT ACAGCAGGCT GCCTAAGAAA AAGGAGAATG AGAAGGTGAA GACCGAAGTC 
GGAAAAGAGG TGGCCGCGGC CCGGCGGAAG CAGCACCTCT CCTCCCTTCA GTACTACTGT 
GCCCTCAACG CGCTGCAGTA CAGAAAGCAA ATGGCCATGA TGGAGCCCAT GATAGGCTTT 
GCCCATGGAC AGATTAACTT TTTTAAGAAG GGAGCAGAGA TGTTTTCCAA ACGTATGGAC 
AGCTTTTTAT CCTCCGTTGC AGACATGGTT CAAAGCATTC AGGTAGAACT GGAAGCCGAG 
GCGGAAAAGA TGCGGGTGTC CCAGCAAGAA TTACTTTCTG TTGATGAATC TGTTTACACT 
CCAGACTCTG ATGTGGCCGC ACCACAGATC AACAGGAACC TCATCCAGAA GGCTGGTTAC 
CTTAATCTTA GAAACAAAAC AGGGCTGGTC ACCACCACCT GGGAGAGGCT TTATTTCTTC 
ACCCAAGGCG GGAATCTCAT GTGTCAGCCC AGGGGAGCCG TGGCTGGAGG TTTGATCCAG 
GACCTGGACA ACTGCTCAGT GATGGCCGTG GATTGCGAAG ACCGGCGCTA CTGCTTCCAG 
ATCACCACGC CCAATGGAAA ATCGGGAATA ATCCTCCAGG CTGAGAGCAG AAAGGAAAAT 
GAAGAGTGGA TATGTGCAAT AAACAACATC TCCAGACAGA TCTACCTGAC CGACAACCCT 
GAGGCAGTCG CGATCAAGTT GAATCAGACC GCTCTGCAAG CAGTGACTCC CATTACAAGT 
TTTGGAAAAA AACAAGAAAG CTCATGCCCC AGCCAGAACC TGAAAAATTC AGAGATGGAA 
AATGAAAATG ACAAGATTGT TCCCAAAGCA ACAGCCAGTC TACCTGAAGC AGAGGAGCTG 
ATCGCGCCTG GAACGCCGAT TCAATTCGAT ATTGTGCTTC CTGCTACAGA ATTCCTTGAT 
CAGAACAGAG GGAGCAGGCG TACCAACCCT TTTGGTGAAA CTGAGGATGA ATCATTTCCA 
GAAGCAGAAG ATTCTCTTTT GCAGCAGATG TTTATAGTTC GGTTTTTGGG ATCAATGGCA 
GTTAAAACAG ACAGCACTAC TGAAGTGATT TATGAAGCGA TGAGACAAGT ATTGGCTGCT 
CGGGCTATTC ATAACATCTT CCGCATGACA GAATCCCATC TGATGGTCAC CAGTCAATCT 
TTGAGGTTGA TAGATCCACA GACTCAAGTA TCAAGGGCCA ATTTTGAACT TACCAGTGTC 
ACACAATTTG CTGCTCATCA AGAAAACAAG AGACTGGTTG GTTTTGTCAT CCGTGTTCCT 
GAATCCACTG GAGAAGAATC TCTGAGTACA TACATTTTTG AAAGCAACTC AGAAGGCGAA 
AAGATATGTT ATGCTATTAA TTTGGGAAAA GAAATTATTG AGGTTCAGAA GGATCCAGAA 
GCACTGGCTC AATTAATGCT GTCCATACCA CTAACCAATG ACGGAAAATA TGTACTGTTA 
AACGATCAAC CAGATGACGA TGATGGAAAT CCAAACGAAC ATAGAGGCGC AGAATCCGAA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875042.1
CDS	3061..4926
Translation	MQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	XM_017019553.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019553.2

ORF Insert Sequence:

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGAGCA TGACCTCTCA 
ATGGCAAAAT ACAGCAGGCT GCCTAAGAAA AAGGAGAATG AGAAGGTGAA GACCGAAGTC 
GGAAAAGAGG TGGCCGCGGC CCGGCGGAAG CAGCACCTCT CCTCCCTTCA GTACTACTGT 
GCCCTCAACG CGCTGCAGTA CAGAAAGCAA ATGGCCATGA TGGAGCCCAT GATAGGCTTT 
GCCCATGGAC AGATTAACTT TTTTAAGAAG GGAGCAGAGA TGTTTTCCAA ACGTATGGAC 
AGCTTTTTAT CCTCCGTTGC AGACATGGTT CAAAGCATTC AGGTAGAACT GGAAGCCGAG 
GCGGAAAAGA TGCGGGTGTC CCAGCAAGAA TTACTTTCTG TTGATGAATC TGTTTACACT 
CCAGACTCTG ATGTGGCCGC ACCACAGATC AACAGGAACC TCATCCAGAA GGCTGGTTAC 
CTTAATCTTA GAAACAAAAC AGGGCTGGTC ACCACCACCT GGGAGAGGCT TTATTTCTTC 
ACCCAAGGCG GGAATCTCAT GTGTCAGCCC AGGGGAGCCG TGGCTGGAGG TTTGATCCAG 
GACCTGGACA ACTGCTCAGT GATGGCCGTG GATTGCGAAG ACCGGCGCTA CTGCTTCCAG 
ATCACCACGC CCAATGGAAA ATCGGGAATA ATCCTCCAGG CTGAGAGCAG AAAGGAAAAT 
GAAGAGTGGA TATGTGCAAT AAACAACATC TCCAGACAGA TCTACCTGAC CGACAACCCT 
GAGGCAGTCG CGATCAAGTT GAATCAGACC GCTCTGCAAG CAGTGACTCC CATTACAAGT 
TTTGGAAAAA AACAAGAAAG CTCATGCCCC AGCCAGAACC TGAAAAATTC AGAGATGGAA 
AATGAAAATG ACAAGATTGT TCCCAAAGCA ACAGCCAGTC TACCTGAAGC AGAGGAGCTG 
ATCGCGCCTG GAACGCCGAT TCAATTCGAT ATTGTGCTTC CTGCTACAGA ATTCCTTGAT 
CAGAACAGAG GGAGCAGGCG TACCAACCCT TTTGGTGAAA CTGAGGATGA ATCATTTCCA 
GAAGCAGAAG ATTCTCTTTT GCAGCAGATG TTTATAGTTC GGTTTTTGGG ATCAATGGCA 
GTTAAAACAG ACAGCACTAC TGAAGTGATT TATGAAGCGA TGAGACAAGT ATTGGCTGCT 
CGGGCTATTC ATAACATCTT CCGCATGACA GAATCCCATC TGATGGTCAC CAGTCAATCT 
TTGAGGTTGA TAGATCCACA GACTCAAGTA TCAAGGGCCA ATTTTGAACT TACCAGTGTC 
ACACAATTTG CTGCTCATCA AGAAAACAAG AGACTGGTTG GTTTTGTCAT CCGTGTTCCT 
GAATCCACTG GAGAAGAATC TCTGAGTACA TACATTTTTG AAAGCAACTC AGAAGGCGAA 
AAGATATGTT ATGCTATTAA TTTGGGAAAA GAAATTATTG AGGTTCAGAA GGATCCAGAA 
GCACTGGCTC AATTAATGCT GTCCATACCA CTAACCAATG ACGGAAAATA TGTACTGTTA 
AACGATCAAC CAGATGACGA TGATGGAAAT CCAAACGAAC ATAGAGGCGC AGAATCCGAA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_017019553.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875042.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu15669
Clone ID Related Accession (Same CDS sequence)	XM_017019553.2 , XM_017019553.1 , XM_017019552.2 , XM_017019552.1 , NM_001251905.1
Accession Version	XM_017019553.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGAGCA TGACCTCTCA 
ATGGCAAAAT ACAGCAGGCT GCCTAAGAAA AAGGAGAATG AGAAGGTGAA GACCGAAGTC 
GGAAAAGAGG TGGCCGCGGC CCGGCGGAAG CAGCACCTCT CCTCCCTTCA GTACTACTGT 
GCCCTCAACG CGCTGCAGTA CAGAAAGCAA ATGGCCATGA TGGAGCCCAT GATAGGCTTT 
GCCCATGGAC AGATTAACTT TTTTAAGAAG GGAGCAGAGA TGTTTTCCAA ACGTATGGAC 
AGCTTTTTAT CCTCCGTTGC AGACATGGTT CAAAGCATTC AGGTAGAACT GGAAGCCGAG 
GCGGAAAAGA TGCGGGTGTC CCAGCAAGAA TTACTTTCTG TTGATGAATC TGTTTACACT 
CCAGACTCTG ATGTGGCCGC ACCACAGATC AACAGGAACC TCATCCAGAA GGCTGGTTAC 
CTTAATCTTA GAAACAAAAC AGGGCTGGTC ACCACCACCT GGGAGAGGCT TTATTTCTTC 
ACCCAAGGCG GGAATCTCAT GTGTCAGCCC AGGGGAGCCG TGGCTGGAGG TTTGATCCAG 
GACCTGGACA ACTGCTCAGT GATGGCCGTG GATTGCGAAG ACCGGCGCTA CTGCTTCCAG 
ATCACCACGC CCAATGGAAA ATCGGGAATA ATCCTCCAGG CTGAGAGCAG AAAGGAAAAT 
GAAGAGTGGA TATGTGCAAT AAACAACATC TCCAGACAGA TCTACCTGAC CGACAACCCT 
GAGGCAGTCG CGATCAAGTT GAATCAGACC GCTCTGCAAG CAGTGACTCC CATTACAAGT 
TTTGGAAAAA AACAAGAAAG CTCATGCCCC AGCCAGAACC TGAAAAATTC AGAGATGGAA 
AATGAAAATG ACAAGATTGT TCCCAAAGCA ACAGCCAGTC TACCTGAAGC AGAGGAGCTG 
ATCGCGCCTG GAACGCCGAT TCAATTCGAT ATTGTGCTTC CTGCTACAGA ATTCCTTGAT 
CAGAACAGAG GGAGCAGGCG TACCAACCCT TTTGGTGAAA CTGAGGATGA ATCATTTCCA 
GAAGCAGAAG ATTCTCTTTT GCAGCAGATG TTTATAGTTC GGTTTTTGGG ATCAATGGCA 
GTTAAAACAG ACAGCACTAC TGAAGTGATT TATGAAGCGA TGAGACAAGT ATTGGCTGCT 
CGGGCTATTC ATAACATCTT CCGCATGACA GAATCCCATC TGATGGTCAC CAGTCAATCT 
TTGAGGTTGA TAGATCCACA GACTCAAGTA TCAAGGGCCA ATTTTGAACT TACCAGTGTC 
ACACAATTTG CTGCTCATCA AGAAAACAAG AGACTGGTTG GTTTTGTCAT CCGTGTTCCT 
GAATCCACTG GAGAAGAATC TCTGAGTACA TACATTTTTG AAAGCAACTC AGAAGGCGAA 
AAGATATGTT ATGCTATTAA TTTGGGAAAA GAAATTATTG AGGTTCAGAA GGATCCAGAA 
GCACTGGCTC AATTAATGCT GTCCATACCA CTAACCAATG ACGGAAAATA TGTACTGTTA 
AACGATCAAC CAGATGACGA TGATGGAAAT CCAAACGAAC ATAGAGGCGC AGAATCCGAA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875042.1
CDS	3061..4926
Translation	MQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	XM_017019553.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019553.1

ORF Insert Sequence:

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGAGCA TGACCTCTCA 
ATGGCAAAAT ACAGCAGGCT GCCTAAGAAA AAGGAGAATG AGAAGGTGAA GACCGAAGTC 
GGAAAAGAGG TGGCCGCGGC CCGGCGGAAG CAGCACCTCT CCTCCCTTCA GTACTACTGT 
GCCCTCAACG CGCTGCAGTA CAGAAAGCAA ATGGCCATGA TGGAGCCCAT GATAGGCTTT 
GCCCATGGAC AGATTAACTT TTTTAAGAAG GGAGCAGAGA TGTTTTCCAA ACGTATGGAC 
AGCTTTTTAT CCTCCGTTGC AGACATGGTT CAAAGCATTC AGGTAGAACT GGAAGCCGAG 
GCGGAAAAGA TGCGGGTGTC CCAGCAAGAA TTACTTTCTG TTGATGAATC TGTTTACACT 
CCAGACTCTG ATGTGGCCGC ACCACAGATC AACAGGAACC TCATCCAGAA GGCTGGTTAC 
CTTAATCTTA GAAACAAAAC AGGGCTGGTC ACCACCACCT GGGAGAGGCT TTATTTCTTC 
ACCCAAGGCG GGAATCTCAT GTGTCAGCCC AGGGGAGCCG TGGCTGGAGG TTTGATCCAG 
GACCTGGACA ACTGCTCAGT GATGGCCGTG GATTGCGAAG ACCGGCGCTA CTGCTTCCAG 
ATCACCACGC CCAATGGAAA ATCGGGAATA ATCCTCCAGG CTGAGAGCAG AAAGGAAAAT 
GAAGAGTGGA TATGTGCAAT AAACAACATC TCCAGACAGA TCTACCTGAC CGACAACCCT 
GAGGCAGTCG CGATCAAGTT GAATCAGACC GCTCTGCAAG CAGTGACTCC CATTACAAGT 
TTTGGAAAAA AACAAGAAAG CTCATGCCCC AGCCAGAACC TGAAAAATTC AGAGATGGAA 
AATGAAAATG ACAAGATTGT TCCCAAAGCA ACAGCCAGTC TACCTGAAGC AGAGGAGCTG 
ATCGCGCCTG GAACGCCGAT TCAATTCGAT ATTGTGCTTC CTGCTACAGA ATTCCTTGAT 
CAGAACAGAG GGAGCAGGCG TACCAACCCT TTTGGTGAAA CTGAGGATGA ATCATTTCCA 
GAAGCAGAAG ATTCTCTTTT GCAGCAGATG TTTATAGTTC GGTTTTTGGG ATCAATGGCA 
GTTAAAACAG ACAGCACTAC TGAAGTGATT TATGAAGCGA TGAGACAAGT ATTGGCTGCT 
CGGGCTATTC ATAACATCTT CCGCATGACA GAATCCCATC TGATGGTCAC CAGTCAATCT 
TTGAGGTTGA TAGATCCACA GACTCAAGTA TCAAGGGCCA ATTTTGAACT TACCAGTGTC 
ACACAATTTG CTGCTCATCA AGAAAACAAG AGACTGGTTG GTTTTGTCAT CCGTGTTCCT 
GAATCCACTG GAGAAGAATC TCTGAGTACA TACATTTTTG AAAGCAACTC AGAAGGCGAA 
AAGATATGTT ATGCTATTAA TTTGGGAAAA GAAATTATTG AGGTTCAGAA GGATCCAGAA 
GCACTGGCTC AATTAATGCT GTCCATACCA CTAACCAATG ACGGAAAATA TGTACTGTTA 
AACGATCAAC CAGATGACGA TGATGGAAAT CCAAACGAAC ATAGAGGCGC AGAATCCGAA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_017019552.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875041.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu15669
Clone ID Related Accession (Same CDS sequence)	XM_017019553.2 , XM_017019553.1 , XM_017019552.2 , XM_017019552.1 , NM_001251905.1
Accession Version	XM_017019552.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017019552.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGAGCA TGACCTCTCA 
ATGGCAAAAT ACAGCAGGCT GCCTAAGAAA AAGGAGAATG AGAAGGTGAA GACCGAAGTC 
GGAAAAGAGG TGGCCGCGGC CCGGCGGAAG CAGCACCTCT CCTCCCTTCA GTACTACTGT 
GCCCTCAACG CGCTGCAGTA CAGAAAGCAA ATGGCCATGA TGGAGCCCAT GATAGGCTTT 
GCCCATGGAC AGATTAACTT TTTTAAGAAG GGAGCAGAGA TGTTTTCCAA ACGTATGGAC 
AGCTTTTTAT CCTCCGTTGC AGACATGGTT CAAAGCATTC AGGTAGAACT GGAAGCCGAG 
GCGGAAAAGA TGCGGGTGTC CCAGCAAGAA TTACTTTCTG TTGATGAATC TGTTTACACT 
CCAGACTCTG ATGTGGCCGC ACCACAGATC AACAGGAACC TCATCCAGAA GGCTGGTTAC 
CTTAATCTTA GAAACAAAAC AGGGCTGGTC ACCACCACCT GGGAGAGGCT TTATTTCTTC 
ACCCAAGGCG GGAATCTCAT GTGTCAGCCC AGGGGAGCCG TGGCTGGAGG TTTGATCCAG 
GACCTGGACA ACTGCTCAGT GATGGCCGTG GATTGCGAAG ACCGGCGCTA CTGCTTCCAG 
ATCACCACGC CCAATGGAAA ATCGGGAATA ATCCTCCAGG CTGAGAGCAG AAAGGAAAAT 
GAAGAGTGGA TATGTGCAAT AAACAACATC TCCAGACAGA TCTACCTGAC CGACAACCCT 
GAGGCAGTCG CGATCAAGTT GAATCAGACC GCTCTGCAAG CAGTGACTCC CATTACAAGT 
TTTGGAAAAA AACAAGAAAG CTCATGCCCC AGCCAGAACC TGAAAAATTC AGAGATGGAA 
AATGAAAATG ACAAGATTGT TCCCAAAGCA ACAGCCAGTC TACCTGAAGC AGAGGAGCTG 
ATCGCGCCTG GAACGCCGAT TCAATTCGAT ATTGTGCTTC CTGCTACAGA ATTCCTTGAT 
CAGAACAGAG GGAGCAGGCG TACCAACCCT TTTGGTGAAA CTGAGGATGA ATCATTTCCA 
GAAGCAGAAG ATTCTCTTTT GCAGCAGATG TTTATAGTTC GGTTTTTGGG ATCAATGGCA 
GTTAAAACAG ACAGCACTAC TGAAGTGATT TATGAAGCGA TGAGACAAGT ATTGGCTGCT 
CGGGCTATTC ATAACATCTT CCGCATGACA GAATCCCATC TGATGGTCAC CAGTCAATCT 
TTGAGGTTGA TAGATCCACA GACTCAAGTA TCAAGGGCCA ATTTTGAACT TACCAGTGTC 
ACACAATTTG CTGCTCATCA AGAAAACAAG AGACTGGTTG GTTTTGTCAT CCGTGTTCCT 
GAATCCACTG GAGAAGAATC TCTGAGTACA TACATTTTTG AAAGCAACTC AGAAGGCGAA 
AAGATATGTT ATGCTATTAA TTTGGGAAAA GAAATTATTG AGGTTCAGAA GGATCCAGAA 
GCACTGGCTC AATTAATGCT GTCCATACCA CTAACCAATG ACGGAAAATA TGTACTGTTA 
AACGATCAAC CAGATGACGA TGATGGAAAT CCAAACGAAC ATAGAGGCGC AGAATCCGAA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875041.1
CDS	1278..3143
Translation	MQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	XM_017019552.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019552.2

ORF Insert Sequence:

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGAGCA TGACCTCTCA 
ATGGCAAAAT ACAGCAGGCT GCCTAAGAAA AAGGAGAATG AGAAGGTGAA GACCGAAGTC 
GGAAAAGAGG TGGCCGCGGC CCGGCGGAAG CAGCACCTCT CCTCCCTTCA GTACTACTGT 
GCCCTCAACG CGCTGCAGTA CAGAAAGCAA ATGGCCATGA TGGAGCCCAT GATAGGCTTT 
GCCCATGGAC AGATTAACTT TTTTAAGAAG GGAGCAGAGA TGTTTTCCAA ACGTATGGAC 
AGCTTTTTAT CCTCCGTTGC AGACATGGTT CAAAGCATTC AGGTAGAACT GGAAGCCGAG 
GCGGAAAAGA TGCGGGTGTC CCAGCAAGAA TTACTTTCTG TTGATGAATC TGTTTACACT 
CCAGACTCTG ATGTGGCCGC ACCACAGATC AACAGGAACC TCATCCAGAA GGCTGGTTAC 
CTTAATCTTA GAAACAAAAC AGGGCTGGTC ACCACCACCT GGGAGAGGCT TTATTTCTTC 
ACCCAAGGCG GGAATCTCAT GTGTCAGCCC AGGGGAGCCG TGGCTGGAGG TTTGATCCAG 
GACCTGGACA ACTGCTCAGT GATGGCCGTG GATTGCGAAG ACCGGCGCTA CTGCTTCCAG 
ATCACCACGC CCAATGGAAA ATCGGGAATA ATCCTCCAGG CTGAGAGCAG AAAGGAAAAT 
GAAGAGTGGA TATGTGCAAT AAACAACATC TCCAGACAGA TCTACCTGAC CGACAACCCT 
GAGGCAGTCG CGATCAAGTT GAATCAGACC GCTCTGCAAG CAGTGACTCC CATTACAAGT 
TTTGGAAAAA AACAAGAAAG CTCATGCCCC AGCCAGAACC TGAAAAATTC AGAGATGGAA 
AATGAAAATG ACAAGATTGT TCCCAAAGCA ACAGCCAGTC TACCTGAAGC AGAGGAGCTG 
ATCGCGCCTG GAACGCCGAT TCAATTCGAT ATTGTGCTTC CTGCTACAGA ATTCCTTGAT 
CAGAACAGAG GGAGCAGGCG TACCAACCCT TTTGGTGAAA CTGAGGATGA ATCATTTCCA 
GAAGCAGAAG ATTCTCTTTT GCAGCAGATG TTTATAGTTC GGTTTTTGGG ATCAATGGCA 
GTTAAAACAG ACAGCACTAC TGAAGTGATT TATGAAGCGA TGAGACAAGT ATTGGCTGCT 
CGGGCTATTC ATAACATCTT CCGCATGACA GAATCCCATC TGATGGTCAC CAGTCAATCT 
TTGAGGTTGA TAGATCCACA GACTCAAGTA TCAAGGGCCA ATTTTGAACT TACCAGTGTC 
ACACAATTTG CTGCTCATCA AGAAAACAAG AGACTGGTTG GTTTTGTCAT CCGTGTTCCT 
GAATCCACTG GAGAAGAATC TCTGAGTACA TACATTTTTG AAAGCAACTC AGAAGGCGAA 
AAGATATGTT ATGCTATTAA TTTGGGAAAA GAAATTATTG AGGTTCAGAA GGATCCAGAA 
GCACTGGCTC AATTAATGCT GTCCATACCA CTAACCAATG ACGGAAAATA TGTACTGTTA 
AACGATCAAC CAGATGACGA TGATGGAAAT CCAAACGAAC ATAGAGGCGC AGAATCCGAA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_017019552.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875041.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu15669
Clone ID Related Accession (Same CDS sequence)	XM_017019553.2 , XM_017019553.1 , XM_017019552.2 , XM_017019552.1 , NM_001251905.1
Accession Version	XM_017019552.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGAGCA TGACCTCTCA 
ATGGCAAAAT ACAGCAGGCT GCCTAAGAAA AAGGAGAATG AGAAGGTGAA GACCGAAGTC 
GGAAAAGAGG TGGCCGCGGC CCGGCGGAAG CAGCACCTCT CCTCCCTTCA GTACTACTGT 
GCCCTCAACG CGCTGCAGTA CAGAAAGCAA ATGGCCATGA TGGAGCCCAT GATAGGCTTT 
GCCCATGGAC AGATTAACTT TTTTAAGAAG GGAGCAGAGA TGTTTTCCAA ACGTATGGAC 
AGCTTTTTAT CCTCCGTTGC AGACATGGTT CAAAGCATTC AGGTAGAACT GGAAGCCGAG 
GCGGAAAAGA TGCGGGTGTC CCAGCAAGAA TTACTTTCTG TTGATGAATC TGTTTACACT 
CCAGACTCTG ATGTGGCCGC ACCACAGATC AACAGGAACC TCATCCAGAA GGCTGGTTAC 
CTTAATCTTA GAAACAAAAC AGGGCTGGTC ACCACCACCT GGGAGAGGCT TTATTTCTTC 
ACCCAAGGCG GGAATCTCAT GTGTCAGCCC AGGGGAGCCG TGGCTGGAGG TTTGATCCAG 
GACCTGGACA ACTGCTCAGT GATGGCCGTG GATTGCGAAG ACCGGCGCTA CTGCTTCCAG 
ATCACCACGC CCAATGGAAA ATCGGGAATA ATCCTCCAGG CTGAGAGCAG AAAGGAAAAT 
GAAGAGTGGA TATGTGCAAT AAACAACATC TCCAGACAGA TCTACCTGAC CGACAACCCT 
GAGGCAGTCG CGATCAAGTT GAATCAGACC GCTCTGCAAG CAGTGACTCC CATTACAAGT 
TTTGGAAAAA AACAAGAAAG CTCATGCCCC AGCCAGAACC TGAAAAATTC AGAGATGGAA 
AATGAAAATG ACAAGATTGT TCCCAAAGCA ACAGCCAGTC TACCTGAAGC AGAGGAGCTG 
ATCGCGCCTG GAACGCCGAT TCAATTCGAT ATTGTGCTTC CTGCTACAGA ATTCCTTGAT 
CAGAACAGAG GGAGCAGGCG TACCAACCCT TTTGGTGAAA CTGAGGATGA ATCATTTCCA 
GAAGCAGAAG ATTCTCTTTT GCAGCAGATG TTTATAGTTC GGTTTTTGGG ATCAATGGCA 
GTTAAAACAG ACAGCACTAC TGAAGTGATT TATGAAGCGA TGAGACAAGT ATTGGCTGCT 
CGGGCTATTC ATAACATCTT CCGCATGACA GAATCCCATC TGATGGTCAC CAGTCAATCT 
TTGAGGTTGA TAGATCCACA GACTCAAGTA TCAAGGGCCA ATTTTGAACT TACCAGTGTC 
ACACAATTTG CTGCTCATCA AGAAAACAAG AGACTGGTTG GTTTTGTCAT CCGTGTTCCT 
GAATCCACTG GAGAAGAATC TCTGAGTACA TACATTTTTG AAAGCAACTC AGAAGGCGAA 
AAGATATGTT ATGCTATTAA TTTGGGAAAA GAAATTATTG AGGTTCAGAA GGATCCAGAA 
GCACTGGCTC AATTAATGCT GTCCATACCA CTAACCAATG ACGGAAAATA TGTACTGTTA 
AACGATCAAC CAGATGACGA TGATGGAAAT CCAAACGAAC ATAGAGGCGC AGAATCCGAA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875041.1
CDS	1463..3328
Translation	MQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	XM_017019552.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019552.1

ORF Insert Sequence:

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGAGCA TGACCTCTCA 
ATGGCAAAAT ACAGCAGGCT GCCTAAGAAA AAGGAGAATG AGAAGGTGAA GACCGAAGTC 
GGAAAAGAGG TGGCCGCGGC CCGGCGGAAG CAGCACCTCT CCTCCCTTCA GTACTACTGT 
GCCCTCAACG CGCTGCAGTA CAGAAAGCAA ATGGCCATGA TGGAGCCCAT GATAGGCTTT 
GCCCATGGAC AGATTAACTT TTTTAAGAAG GGAGCAGAGA TGTTTTCCAA ACGTATGGAC 
AGCTTTTTAT CCTCCGTTGC AGACATGGTT CAAAGCATTC AGGTAGAACT GGAAGCCGAG 
GCGGAAAAGA TGCGGGTGTC CCAGCAAGAA TTACTTTCTG TTGATGAATC TGTTTACACT 
CCAGACTCTG ATGTGGCCGC ACCACAGATC AACAGGAACC TCATCCAGAA GGCTGGTTAC 
CTTAATCTTA GAAACAAAAC AGGGCTGGTC ACCACCACCT GGGAGAGGCT TTATTTCTTC 
ACCCAAGGCG GGAATCTCAT GTGTCAGCCC AGGGGAGCCG TGGCTGGAGG TTTGATCCAG 
GACCTGGACA ACTGCTCAGT GATGGCCGTG GATTGCGAAG ACCGGCGCTA CTGCTTCCAG 
ATCACCACGC CCAATGGAAA ATCGGGAATA ATCCTCCAGG CTGAGAGCAG AAAGGAAAAT 
GAAGAGTGGA TATGTGCAAT AAACAACATC TCCAGACAGA TCTACCTGAC CGACAACCCT 
GAGGCAGTCG CGATCAAGTT GAATCAGACC GCTCTGCAAG CAGTGACTCC CATTACAAGT 
TTTGGAAAAA AACAAGAAAG CTCATGCCCC AGCCAGAACC TGAAAAATTC AGAGATGGAA 
AATGAAAATG ACAAGATTGT TCCCAAAGCA ACAGCCAGTC TACCTGAAGC AGAGGAGCTG 
ATCGCGCCTG GAACGCCGAT TCAATTCGAT ATTGTGCTTC CTGCTACAGA ATTCCTTGAT 
CAGAACAGAG GGAGCAGGCG TACCAACCCT TTTGGTGAAA CTGAGGATGA ATCATTTCCA 
GAAGCAGAAG ATTCTCTTTT GCAGCAGATG TTTATAGTTC GGTTTTTGGG ATCAATGGCA 
GTTAAAACAG ACAGCACTAC TGAAGTGATT TATGAAGCGA TGAGACAAGT ATTGGCTGCT 
CGGGCTATTC ATAACATCTT CCGCATGACA GAATCCCATC TGATGGTCAC CAGTCAATCT 
TTGAGGTTGA TAGATCCACA GACTCAAGTA TCAAGGGCCA ATTTTGAACT TACCAGTGTC 
ACACAATTTG CTGCTCATCA AGAAAACAAG AGACTGGTTG GTTTTGTCAT CCGTGTTCCT 
GAATCCACTG GAGAAGAATC TCTGAGTACA TACATTTTTG AAAGCAACTC AGAAGGCGAA 
AAGATATGTT ATGCTATTAA TTTGGGAAAA GAAATTATTG AGGTTCAGAA GGATCCAGAA 
GCACTGGCTC AATTAATGCT GTCCATACCA CTAACCAATG ACGGAAAATA TGTACTGTTA 
AACGATCAAC CAGATGACGA TGATGGAAAT CCAAACGAAC ATAGAGGCGC AGAATCCGAA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( NM_018171.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060641.2 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 1, mRNA.

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CloneID	OHu17777
Clone ID Related Accession (Same CDS sequence)	NM_018171.4 , NM_018171.3 , NM_018171.5
Accession Version	NM_018171.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY113704.1 and BC028008.1. On Nov 22, 2018 this sequence version replaced NM_018171.3. Transcript Variant: This variant (1) encodes the predominant isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033731.1, AY113704.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCGCCG TGGACAAGCT CCTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GCGTGTTTGA AGAAGATGCT GGCACCCTCA CAGACTATAC CAACCAGCTG 
CTCCAGGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAGA TGTGCCTGGC CACACAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAACTTTG CTCTTGGCAA AGGTGATGAA 
GAAGTAATTT CAACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TCTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CTATCATACA ATTCCGAGAA 
AAGGATCTCA CAGAAGTAAG CACTTTAAAG GATCTATTTG GACTCGCTAG CAATGAGCAT 
GACCTCTCAA TGGCAAAATA CAGCAGGCTG CCTAAGAAAA AGGAGAATGA GAAGGTGAAG 
ACCGAAGTCG GAAAAGAGGT GGCCGCGGCC CGGCGGAAGC AGCACCTCTC CTCCCTTCAG 
TACTACTGTG CCCTCAACGC GCTGCAGTAC AGAAAGCAAA TGGCCATGAT GGAGCCCATG 
ATAGGCTTTG CCCATGGACA GATTAACTTT TTTAAGAAGG GAGCAGAGAT GTTTTCCAAA 
CGTATGGACA GCTTTTTATC CTCCGTTGCA GACATGGTTC AAAGCATTCA GGTAGAACTG 
GAAGCCGAGG CGGAAAAGAT GCGGGTGTCC CAGCAAGAAT TACTTTCTGT TGATGAATCT 
GTTTACACTC CAGACTCTGA TGTGGCCGCA CCACAGATCA ACAGGAACCT CATCCAGAAG 
GCTGGTTACC TTAATCTTAG AAACAAAACA GGGCTGGTCA CCACCACCTG GGAGAGGCTT 
TATTTCTTCA CCCAAGGCGG GAATCTCATG TGTCAGCCCA GGGGAGCCGT GGCTGGAGGT 
TTGATCCAGG ACCTGGACAA CTGCTCAGTG ATGGCCGTGG ATTGCGAAGA CCGGCGCTAC 
TGCTTCCAGA TCACCACGCC CAATGGAAAA TCGGGAATAA TCCTCCAGGC TGAGAGCAGA 
AAGGAAAATG AAGAGTGGAT ATGTGCAATA AACAACATCT CCAGACAGAT CTACCTGACC 
GACAACCCTG AGGCAGTCGC GATCAAGTTG AATCAGACCG CTCTGCAAGC AGTGACTCCC 
ATTACAAGTT TTGGAAAAAA ACAAGAAAGC TCATGCCCCA GCCAGAACCT GAAAAATTCA 
GAGATGGAAA ATGAAAATGA CAAGATTGTT CCCAAAGCAA CAGCCAGTCT ACCTGAAGCA 
GAGGAGCTGA TCGCGCCTGG AACGCCGATT CAATTCGATA TTGTGCTTCC TGCTACAGAA 
TTCCTTGATC AGAACAGAGG GAGCAGGCGT ACCAACCCTT TTGGTGAAAC TGAGGATGAA 
TCATTTCCAG AAGCAGAAGA TTCTCTTTTG CAGCAGATGT TTATAGTTCG GTTTTTGGGA 
TCAATGGCAG TTAAAACAGA CAGCACTACT GAAGTGATTT ATGAAGCGAT GAGACAAGTA 
TTGGCTGCTC GGGCTATTCA TAACATCTTC CGCATGACAG AATCCCATCT GATGGTCACC 
AGTCAATCTT TGAGGTTGAT AGATCCACAG ACTCAAGTAT CAAGGGCCAA TTTTGAACTT 
ACCAGTGTCA CACAATTTGC TGCTCATCAA GAAAACAAGA GACTGGTTGG TTTTGTCATC 
CGTGTTCCTG AATCCACTGG AGAAGAATCT CTGAGTACAT ACATTTTTGA AAGCAACTCA 
GAAGGCGAAA AGATATGTTA TGCTATTAAT TTGGGAAAAG AAATTATTGA GGTTCAGAAG 
GATCCAGAAG CACTGGCTCA ATTAATGCTG TCCATACCAC TAACCAATGA CGGAAAATAT 
GTACTGTTAA ACGATCAACC AGATGACGAT GATGGAAATC CAAACGAACA TAGAGGCGCA 
GAATCCGAAG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060641.2
CDS	163..2157
Translation	MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	NM_018171.4
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018171.4

ORF Insert Sequence:

ATGCCCGCCG TGGACAAGCT CCTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GCGTGTTTGA AGAAGATGCT GGCACCCTCA CAGACTATAC CAACCAGCTG 
CTCCAGGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAGA TGTGCCTGGC CACACAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAACTTTG CTCTTGGCAA AGGTGATGAA 
GAAGTAATTT CAACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TCTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CTATCATACA ATTCCGAGAA 
AAGGATCTCA CAGAAGTAAG CACTTTAAAG GATCTATTTG GACTCGCTAG CAATGAGCAT 
GACCTCTCAA TGGCAAAATA CAGCAGGCTG CCTAAGAAAA AGGAGAATGA GAAGGTGAAG 
ACCGAAGTCG GAAAAGAGGT GGCCGCGGCC CGGCGGAAGC AGCACCTCTC CTCCCTTCAG 
TACTACTGTG CCCTCAACGC GCTGCAGTAC AGAAAGCAAA TGGCCATGAT GGAGCCCATG 
ATAGGCTTTG CCCATGGACA GATTAACTTT TTTAAGAAGG GAGCAGAGAT GTTTTCCAAA 
CGTATGGACA GCTTTTTATC CTCCGTTGCA GACATGGTTC AAAGCATTCA GGTAGAACTG 
GAAGCCGAGG CGGAAAAGAT GCGGGTGTCC CAGCAAGAAT TACTTTCTGT TGATGAATCT 
GTTTACACTC CAGACTCTGA TGTGGCCGCA CCACAGATCA ACAGGAACCT CATCCAGAAG 
GCTGGTTACC TTAATCTTAG AAACAAAACA GGGCTGGTCA CCACCACCTG GGAGAGGCTT 
TATTTCTTCA CCCAAGGCGG GAATCTCATG TGTCAGCCCA GGGGAGCCGT GGCTGGAGGT 
TTGATCCAGG ACCTGGACAA CTGCTCAGTG ATGGCCGTGG ATTGCGAAGA CCGGCGCTAC 
TGCTTCCAGA TCACCACGCC CAATGGAAAA TCGGGAATAA TCCTCCAGGC TGAGAGCAGA 
AAGGAAAATG AAGAGTGGAT ATGTGCAATA AACAACATCT CCAGACAGAT CTACCTGACC 
GACAACCCTG AGGCAGTCGC GATCAAGTTG AATCAGACCG CTCTGCAAGC AGTGACTCCC 
ATTACAAGTT TTGGAAAAAA ACAAGAAAGC TCATGCCCCA GCCAGAACCT GAAAAATTCA 
GAGATGGAAA ATGAAAATGA CAAGATTGTT CCCAAAGCAA CAGCCAGTCT ACCTGAAGCA 
GAGGAGCTGA TCGCGCCTGG AACGCCGATT CAATTCGATA TTGTGCTTCC TGCTACAGAA 
TTCCTTGATC AGAACAGAGG GAGCAGGCGT ACCAACCCTT TTGGTGAAAC TGAGGATGAA 
TCATTTCCAG AAGCAGAAGA TTCTCTTTTG CAGCAGATGT TTATAGTTCG GTTTTTGGGA 
TCAATGGCAG TTAAAACAGA CAGCACTACT GAAGTGATTT ATGAAGCGAT GAGACAAGTA 
TTGGCTGCTC GGGCTATTCA TAACATCTTC CGCATGACAG AATCCCATCT GATGGTCACC 
AGTCAATCTT TGAGGTTGAT AGATCCACAG ACTCAAGTAT CAAGGGCCAA TTTTGAACTT 
ACCAGTGTCA CACAATTTGC TGCTCATCAA GAAAACAAGA GACTGGTTGG TTTTGTCATC 
CGTGTTCCTG AATCCACTGG AGAAGAATCT CTGAGTACAT ACATTTTTGA AAGCAACTCA 
GAAGGCGAAA AGATATGTTA TGCTATTAAT TTGGGAAAAG AAATTATTGA GGTTCAGAAG 
GATCCAGAAG CACTGGCTCA ATTAATGCTG TCCATACCAC TAACCAATGA CGGAAAATAT 
GTACTGTTAA ACGATCAACC AGATGACGAT GATGGAAATC CAAACGAACA TAGAGGCGCA 
GAATCCGAAG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( NM_018171.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060641.2 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 1, mRNA.

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CloneID	OHu17777
Clone ID Related Accession (Same CDS sequence)	NM_018171.4 , NM_018171.3 , NM_018171.5
Accession Version	NM_018171.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-10
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY113704.1 and BC028008.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2005 this sequence version replaced NM_018171.2. Transcript Variant: This variant (1) encodes the predominant isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY113704.1, BC033731.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGCCG TGGACAAGCT CCTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GCGTGTTTGA AGAAGATGCT GGCACCCTCA CAGACTATAC CAACCAGCTG 
CTCCAGGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAGA TGTGCCTGGC CACACAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAACTTTG CTCTTGGCAA AGGTGATGAA 
GAAGTAATTT CAACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TCTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CTATCATACA ATTCCGAGAA 
AAGGATCTCA CAGAAGTAAG CACTTTAAAG GATCTATTTG GACTCGCTAG CAATGAGCAT 
GACCTCTCAA TGGCAAAATA CAGCAGGCTG CCTAAGAAAA AGGAGAATGA GAAGGTGAAG 
ACCGAAGTCG GAAAAGAGGT GGCCGCGGCC CGGCGGAAGC AGCACCTCTC CTCCCTTCAG 
TACTACTGTG CCCTCAACGC GCTGCAGTAC AGAAAGCAAA TGGCCATGAT GGAGCCCATG 
ATAGGCTTTG CCCATGGACA GATTAACTTT TTTAAGAAGG GAGCAGAGAT GTTTTCCAAA 
CGTATGGACA GCTTTTTATC CTCCGTTGCA GACATGGTTC AAAGCATTCA GGTAGAACTG 
GAAGCCGAGG CGGAAAAGAT GCGGGTGTCC CAGCAAGAAT TACTTTCTGT TGATGAATCT 
GTTTACACTC CAGACTCTGA TGTGGCCGCA CCACAGATCA ACAGGAACCT CATCCAGAAG 
GCTGGTTACC TTAATCTTAG AAACAAAACA GGGCTGGTCA CCACCACCTG GGAGAGGCTT 
TATTTCTTCA CCCAAGGCGG GAATCTCATG TGTCAGCCCA GGGGAGCCGT GGCTGGAGGT 
TTGATCCAGG ACCTGGACAA CTGCTCAGTG ATGGCCGTGG ATTGCGAAGA CCGGCGCTAC 
TGCTTCCAGA TCACCACGCC CAATGGAAAA TCGGGAATAA TCCTCCAGGC TGAGAGCAGA 
AAGGAAAATG AAGAGTGGAT ATGTGCAATA AACAACATCT CCAGACAGAT CTACCTGACC 
GACAACCCTG AGGCAGTCGC GATCAAGTTG AATCAGACCG CTCTGCAAGC AGTGACTCCC 
ATTACAAGTT TTGGAAAAAA ACAAGAAAGC TCATGCCCCA GCCAGAACCT GAAAAATTCA 
GAGATGGAAA ATGAAAATGA CAAGATTGTT CCCAAAGCAA CAGCCAGTCT ACCTGAAGCA 
GAGGAGCTGA TCGCGCCTGG AACGCCGATT CAATTCGATA TTGTGCTTCC TGCTACAGAA 
TTCCTTGATC AGAACAGAGG GAGCAGGCGT ACCAACCCTT TTGGTGAAAC TGAGGATGAA 
TCATTTCCAG AAGCAGAAGA TTCTCTTTTG CAGCAGATGT TTATAGTTCG GTTTTTGGGA 
TCAATGGCAG TTAAAACAGA CAGCACTACT GAAGTGATTT ATGAAGCGAT GAGACAAGTA 
TTGGCTGCTC GGGCTATTCA TAACATCTTC CGCATGACAG AATCCCATCT GATGGTCACC 
AGTCAATCTT TGAGGTTGAT AGATCCACAG ACTCAAGTAT CAAGGGCCAA TTTTGAACTT 
ACCAGTGTCA CACAATTTGC TGCTCATCAA GAAAACAAGA GACTGGTTGG TTTTGTCATC 
CGTGTTCCTG AATCCACTGG AGAAGAATCT CTGAGTACAT ACATTTTTGA AAGCAACTCA 
GAAGGCGAAA AGATATGTTA TGCTATTAAT TTGGGAAAAG AAATTATTGA GGTTCAGAAG 
GATCCAGAAG CACTGGCTCA ATTAATGCTG TCCATACCAC TAACCAATGA CGGAAAATAT 
GTACTGTTAA ACGATCAACC AGATGACGAT GATGGAAATC CAAACGAACA TAGAGGCGCA 
GAATCCGAAG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060641.2
CDS	219..2213
Translation	MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	NM_018171.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018171.3

ORF Insert Sequence:

ATGCCCGCCG TGGACAAGCT CCTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GCGTGTTTGA AGAAGATGCT GGCACCCTCA CAGACTATAC CAACCAGCTG 
CTCCAGGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAGA TGTGCCTGGC CACACAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAACTTTG CTCTTGGCAA AGGTGATGAA 
GAAGTAATTT CAACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TCTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CTATCATACA ATTCCGAGAA 
AAGGATCTCA CAGAAGTAAG CACTTTAAAG GATCTATTTG GACTCGCTAG CAATGAGCAT 
GACCTCTCAA TGGCAAAATA CAGCAGGCTG CCTAAGAAAA AGGAGAATGA GAAGGTGAAG 
ACCGAAGTCG GAAAAGAGGT GGCCGCGGCC CGGCGGAAGC AGCACCTCTC CTCCCTTCAG 
TACTACTGTG CCCTCAACGC GCTGCAGTAC AGAAAGCAAA TGGCCATGAT GGAGCCCATG 
ATAGGCTTTG CCCATGGACA GATTAACTTT TTTAAGAAGG GAGCAGAGAT GTTTTCCAAA 
CGTATGGACA GCTTTTTATC CTCCGTTGCA GACATGGTTC AAAGCATTCA GGTAGAACTG 
GAAGCCGAGG CGGAAAAGAT GCGGGTGTCC CAGCAAGAAT TACTTTCTGT TGATGAATCT 
GTTTACACTC CAGACTCTGA TGTGGCCGCA CCACAGATCA ACAGGAACCT CATCCAGAAG 
GCTGGTTACC TTAATCTTAG AAACAAAACA GGGCTGGTCA CCACCACCTG GGAGAGGCTT 
TATTTCTTCA CCCAAGGCGG GAATCTCATG TGTCAGCCCA GGGGAGCCGT GGCTGGAGGT 
TTGATCCAGG ACCTGGACAA CTGCTCAGTG ATGGCCGTGG ATTGCGAAGA CCGGCGCTAC 
TGCTTCCAGA TCACCACGCC CAATGGAAAA TCGGGAATAA TCCTCCAGGC TGAGAGCAGA 
AAGGAAAATG AAGAGTGGAT ATGTGCAATA AACAACATCT CCAGACAGAT CTACCTGACC 
GACAACCCTG AGGCAGTCGC GATCAAGTTG AATCAGACCG CTCTGCAAGC AGTGACTCCC 
ATTACAAGTT TTGGAAAAAA ACAAGAAAGC TCATGCCCCA GCCAGAACCT GAAAAATTCA 
GAGATGGAAA ATGAAAATGA CAAGATTGTT CCCAAAGCAA CAGCCAGTCT ACCTGAAGCA 
GAGGAGCTGA TCGCGCCTGG AACGCCGATT CAATTCGATA TTGTGCTTCC TGCTACAGAA 
TTCCTTGATC AGAACAGAGG GAGCAGGCGT ACCAACCCTT TTGGTGAAAC TGAGGATGAA 
TCATTTCCAG AAGCAGAAGA TTCTCTTTTG CAGCAGATGT TTATAGTTCG GTTTTTGGGA 
TCAATGGCAG TTAAAACAGA CAGCACTACT GAAGTGATTT ATGAAGCGAT GAGACAAGTA 
TTGGCTGCTC GGGCTATTCA TAACATCTTC CGCATGACAG AATCCCATCT GATGGTCACC 
AGTCAATCTT TGAGGTTGAT AGATCCACAG ACTCAAGTAT CAAGGGCCAA TTTTGAACTT 
ACCAGTGTCA CACAATTTGC TGCTCATCAA GAAAACAAGA GACTGGTTGG TTTTGTCATC 
CGTGTTCCTG AATCCACTGG AGAAGAATCT CTGAGTACAT ACATTTTTGA AAGCAACTCA 
GAAGGCGAAA AGATATGTTA TGCTATTAAT TTGGGAAAAG AAATTATTGA GGTTCAGAAG 
GATCCAGAAG CACTGGCTCA ATTAATGCTG TCCATACCAC TAACCAATGA CGGAAAATAT 
GTACTGTTAA ACGATCAACC AGATGACGAT GATGGAAATC CAAACGAACA TAGAGGCGCA 
GAATCCGAAG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_006719472.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719535.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu46658
Clone ID Related Accession (Same CDS sequence)	XM_006719472.1
Accession Version	XM_006719472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCCG TGGACAAGCT CCTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GCGTGTTTGA AGAAGATGCT GGCACCCTCA CAGACTATAC CAACCAGCTG 
CTCCAGGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAGA TGTGCCTGGC CACACAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAACTTTG CTCTTGGCAA AGGTGATGAA 
GAAGTAATTT CAACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TCTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CTATCATACA ATTCCGAGAA 
AAGGATCTCA CAGAAGTAAG CACTTTAAAG GATCTATTTG GACTCGCTAG CAATGATGTC 
TGTTTATTTT TAGAGCATGA CCTCTCAATG GCAAAATACA GCAGGCTGCC TAAGAAAAAG 
GAGAATGAGA AGGTGAAGAC CGAAGTCGGA AAAGAGGTGG CCGCGGCCCG GCGGAAGCAG 
CACCTCTCCT CCCTTCAGTA CTACTGTGCC CTCAACGCGC TGCAGTACAG AAAGCAAATG 
GCCATGATGG AGCCCATGAT AGGCTTTGCC CATGGACAGA TTAACTTTTT TAAGAAGGGA 
GCAGAGATGT TTTCCAAACG TATGGACAGC TTTTTATCCT CCGTTGCAGA CATGGTTCAA 
AGCATTCAGG TAGAACTGGA AGCCGAGGCG GAAAAGATGC GGGTGTCCCA GCAAGAATTA 
CTTTCTGTTG ATGAATCTGT TTACACTCCA GACTCTGATG TGGCCGCACC ACAGATCAAC 
AGGAACCTCA TCCAGAAGGC TGGTTACCTT AATCTTAGAA ACAAAACAGG GCTGGTCACC 
ACCACCTGGG AGAGGCTTTA TTTCTTCACC CAAGGCGGGA ATCTCATGTG TCAGCCCAGG 
GGAGCCGTGG CTGGAGGTTT GATCCAGGAC CTGGACAACT GCTCAGTGAT GGCCGTGGAT 
TGCGAAGACC GGCGCTACTG CTTCCAGATC ACCACGCCCA ATGGAAAATC GGGAATAATC 
CTCCAGGCTG AGAGCAGAAA GGAAAATGAA GAGTGGATAT GTGCAATAAA CAACATCTCC 
AGACAGATCT ACCTGACCGA CAACCCTGAG GCAGTCGCGA TCAAGTTGAA TCAGACCGCT 
CTGCAAGCAG TGACTCCCAT TACAAGTTTT GGAAAAAAAC AAGAAAGCTC ATGCCCCAGC 
CAGAACCTGA AAAATTCAGA GATGGAAAAT GAAAATGACA AGATTGTTCC CAAAGCAACA 
GCCAGTCTAC CTGAAGCAGA GGAGCTGATC GCGCCTGGAA CGCCGATTCA ATTCGATATT 
GTGCTTCCTG CTACAGAATT CCTTGATCAG AACAGAGGGA GCAGGCGTAC CAACCCTTTT 
GGTGAAACTG AGGATGAATC ATTTCCAGAA GCAGAAGATT CTCTTTTGCA GCAGATGTTT 
ATAGTTCGGT TTTTGGGATC AATGGCAGTT AAAACAGACA GCACTACTGA AGTGATTTAT 
GAAGCGATGA GACAAGTATT GGCTGCTCGG GCTATTCATA ACATCTTCCG CATGACAGAA 
TCCCATCTGA TGGTCACCAG TCAATCTTTG AGGTTGATAG ATCCACAGAC TCAAGTATCA 
AGGGCCAATA TATGTTATGC TATTAATTTG GGAAAAGAAA TTATTGAGGT TCAGAAGGAT 
CCAGAAGCAC TGGCTCAATT AATGCTGTCC ATACCACTAA CCAATGACGG AAAATATGTA 
CTGTTAAACG ATCAACCAGA TGACGATGAT GGAAATCCAA ACGAACATAG AGGCGCAGAA 
TCCGAAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719535.1
CDS	138..2009
Translation	MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNDVCLFLEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	XM_006719472.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719472.1

ORF Insert Sequence:

ATGCCCGCCG TGGACAAGCT CCTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GCGTGTTTGA AGAAGATGCT GGCACCCTCA CAGACTATAC CAACCAGCTG 
CTCCAGGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAGA TGTGCCTGGC CACACAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAACTTTG CTCTTGGCAA AGGTGATGAA 
GAAGTAATTT CAACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TCTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CTATCATACA ATTCCGAGAA 
AAGGATCTCA CAGAAGTAAG CACTTTAAAG GATCTATTTG GACTCGCTAG CAATGATGTC 
TGTTTATTTT TAGAGCATGA CCTCTCAATG GCAAAATACA GCAGGCTGCC TAAGAAAAAG 
GAGAATGAGA AGGTGAAGAC CGAAGTCGGA AAAGAGGTGG CCGCGGCCCG GCGGAAGCAG 
CACCTCTCCT CCCTTCAGTA CTACTGTGCC CTCAACGCGC TGCAGTACAG AAAGCAAATG 
GCCATGATGG AGCCCATGAT AGGCTTTGCC CATGGACAGA TTAACTTTTT TAAGAAGGGA 
GCAGAGATGT TTTCCAAACG TATGGACAGC TTTTTATCCT CCGTTGCAGA CATGGTTCAA 
AGCATTCAGG TAGAACTGGA AGCCGAGGCG GAAAAGATGC GGGTGTCCCA GCAAGAATTA 
CTTTCTGTTG ATGAATCTGT TTACACTCCA GACTCTGATG TGGCCGCACC ACAGATCAAC 
AGGAACCTCA TCCAGAAGGC TGGTTACCTT AATCTTAGAA ACAAAACAGG GCTGGTCACC 
ACCACCTGGG AGAGGCTTTA TTTCTTCACC CAAGGCGGGA ATCTCATGTG TCAGCCCAGG 
GGAGCCGTGG CTGGAGGTTT GATCCAGGAC CTGGACAACT GCTCAGTGAT GGCCGTGGAT 
TGCGAAGACC GGCGCTACTG CTTCCAGATC ACCACGCCCA ATGGAAAATC GGGAATAATC 
CTCCAGGCTG AGAGCAGAAA GGAAAATGAA GAGTGGATAT GTGCAATAAA CAACATCTCC 
AGACAGATCT ACCTGACCGA CAACCCTGAG GCAGTCGCGA TCAAGTTGAA TCAGACCGCT 
CTGCAAGCAG TGACTCCCAT TACAAGTTTT GGAAAAAAAC AAGAAAGCTC ATGCCCCAGC 
CAGAACCTGA AAAATTCAGA GATGGAAAAT GAAAATGACA AGATTGTTCC CAAAGCAACA 
GCCAGTCTAC CTGAAGCAGA GGAGCTGATC GCGCCTGGAA CGCCGATTCA ATTCGATATT 
GTGCTTCCTG CTACAGAATT CCTTGATCAG AACAGAGGGA GCAGGCGTAC CAACCCTTTT 
GGTGAAACTG AGGATGAATC ATTTCCAGAA GCAGAAGATT CTCTTTTGCA GCAGATGTTT 
ATAGTTCGGT TTTTGGGATC AATGGCAGTT AAAACAGACA GCACTACTGA AGTGATTTAT 
GAAGCGATGA GACAAGTATT GGCTGCTCGG GCTATTCATA ACATCTTCCG CATGACAGAA 
TCCCATCTGA TGGTCACCAG TCAATCTTTG AGGTTGATAG ATCCACAGAC TCAAGTATCA 
AGGGCCAATA TATGTTATGC TATTAATTTG GGAAAAGAAA TTATTGAGGT TCAGAAGGAT 
CCAGAAGCAC TGGCTCAATT AATGCTGTCC ATACCACTAA CCAATGACGG AAAATATGTA 
CTGTTAAACG ATCAACCAGA TGACGATGAT GGAAATCCAA ACGAACATAG AGGCGCAGAA 
TCCGAAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_011538530.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536832.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu69147
Clone ID Related Accession (Same CDS sequence)	XM_011538530.2 , XM_011538530.3
Accession Version	XM_011538530.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACAC CCCAGACTCG CTCTTTACTG AGCGTGTTTG AAGAAGATGC TGGCACCCTC 
ACAGACTATA CCAACCAGCT GCTCCAGGCA ATGCAGCGCG TCTATGGAGC CCAGAATGAG 
ATGTGCCTGG CCACACAACA GCTTTCTAAG CAACTGCTGG CATATGAAAA ACAGAACTTT 
GCTCTTGGCA AAGGTGATGA AGAAGTAATT TCAACACTCC ACTATTTTTC CAAAGTGGTG 
GATGAGCTTA ATCTTCTCCA TACAGAGCTG GCTAAACAGT TGGCAGACAC AATGGTTCTA 
CCTATCATAC AATTCCGAGA AAAGGATCTC ACAGAAGTAA GCACTTTAAA GGATCTATTT 
GGACTCGCTA GCAATGATGT CTGTTTATTT TTAGAGCATG ACCTCTCAAT GGCAAAATAC 
AGCAGGCTGC CTAAGAAAAA GGAGAATGAG AAGGTGAAGA CCGAAGTCGG AAAAGAGGTG 
GCCGCGGCCC GGCGGAAGCA GCACCTCTCC TCCCTTCAGT ACTACTGTGC CCTCAACGCG 
CTGCAGTACA GAAAGCAAAT GGCCATGATG GAGCCCATGA TAGGCTTTGC CCATGGACAG 
ATTAACTTTT TTAAGAAGGG AGCAGAGATG TTTTCCAAAC GTATGGACAG CTTTTTATCC 
TCCGTTGCAG ACATGGTTCA AAGCATTCAG GTAGAACTGG AAGCCGAGGC GGAAAAGATG 
CGGGTGTCCC AGCAAGAATT ACTTTCTGTT GATGAATCTG TTTACACTCC AGACTCTGAT 
GTGGCCGCAC CACAGATCAA CAGGAACCTC ATCCAGAAGG CTGGTTACCT TAATCTTAGA 
AACAAAACAG GGCTGGTCAC CACCACCTGG GAGAGGCTTT ATTTCTTCAC CCAAGGCGGG 
AATCTCATGT GTCAGCCCAG GGGAGCCGTG GCTGGAGGTT TGATCCAGGA CCTGGACAAC 
TGCTCAGTGA TGGCCGTGGA TTGCGAAGAC CGGCGCTACT GCTTCCAGAT CACCACGCCC 
AATGGAAAAT CGGGAATAAT CCTCCAGGCT GAGAGCAGAA AGGAAAATGA AGAGTGGATA 
TGTGCAATAA ACAACATCTC CAGACAGATC TACCTGACCG ACAACCCTGA GGCAGTCGCG 
ATCAAGTTGA ATCAGACCGC TCTGCAAGCA GTGACTCCCA TTACAAGTTT TGGAAAAAAA 
CAAGAAAGCT CATGCCCCAG CCAGAACCTG AAAAATTCAG AGATGGAAAA TGAAAATGAC 
AAGATTGTTC CCAAAGCAAC AGCCAGTCTA CCTGAAGCAG AGGAGCTGAT CGCGCCTGGA 
ACGCCGATTC AATTCGATAT TGTGCTTCCT GCTACAGAAT TCCTTGATCA GAACAGAGGG 
AGCAGGCGTA CCAACCCTTT TGGTGAAACT GAGGATGAAT CATTTCCAGA AGCAGAAGAT 
TCTCTTTTGC AGCAGATGTT TATAGTTCGG TTTTTGGGAT CAATGGCAGT TAAAACAGAC 
AGCACTACTG AAGTGATTTA TGAAGCGATG AGACAAGTAT TGGCTGCTCG GGCTATTCAT 
AACATCTTCC GCATGACAGA ATCCCATCTG ATGGTCACCA GTCAATCTTT GAGGTTGATA 
GATCCACAGA CTCAAGTATC AAGGGCCAAT TTTGAACTTA CCAGTGTCAC ACAATTTGCT 
GCTCATCAAG AAAACAAGAG ACTGGTTGGT TTTGTCATCC GTGTTCCTGA ATCCACTGGA 
GAAGAATCTC TGAGTACATA CATTTTTGAA AGCAACTCAG AAGGCGAAAA GATATGTTAT 
GCTATTAATT TGGGAAAAGA AATTATTGAG GTTCAGAAGG ATCCAGAAGC ACTGGCTCAA 
TTAATGCTGT CCATACCACT AACCAATGAC GGAAAATATG TACTGTTAAA CGATCAACCA 
GATGACGATG ATGGAAATCC AAACGAACAT AGAGGCGCAG AATCCGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536832.1
CDS	1710..3683
Translation	MTTPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNDVCLFLEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	XM_011538530.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538530.2

ORF Insert Sequence:

ATGACCACAC CCCAGACTCG CTCTTTACTG AGCGTGTTTG AAGAAGATGC TGGCACCCTC 
ACAGACTATA CCAACCAGCT GCTCCAGGCA ATGCAGCGCG TCTATGGAGC CCAGAATGAG 
ATGTGCCTGG CCACACAACA GCTTTCTAAG CAACTGCTGG CATATGAAAA ACAGAACTTT 
GCTCTTGGCA AAGGTGATGA AGAAGTAATT TCAACACTCC ACTATTTTTC CAAAGTGGTG 
GATGAGCTTA ATCTTCTCCA TACAGAGCTG GCTAAACAGT TGGCAGACAC AATGGTTCTA 
CCTATCATAC AATTCCGAGA AAAGGATCTC ACAGAAGTAA GCACTTTAAA GGATCTATTT 
GGACTCGCTA GCAATGATGT CTGTTTATTT TTAGAGCATG ACCTCTCAAT GGCAAAATAC 
AGCAGGCTGC CTAAGAAAAA GGAGAATGAG AAGGTGAAGA CCGAAGTCGG AAAAGAGGTG 
GCCGCGGCCC GGCGGAAGCA GCACCTCTCC TCCCTTCAGT ACTACTGTGC CCTCAACGCG 
CTGCAGTACA GAAAGCAAAT GGCCATGATG GAGCCCATGA TAGGCTTTGC CCATGGACAG 
ATTAACTTTT TTAAGAAGGG AGCAGAGATG TTTTCCAAAC GTATGGACAG CTTTTTATCC 
TCCGTTGCAG ACATGGTTCA AAGCATTCAG GTAGAACTGG AAGCCGAGGC GGAAAAGATG 
CGGGTGTCCC AGCAAGAATT ACTTTCTGTT GATGAATCTG TTTACACTCC AGACTCTGAT 
GTGGCCGCAC CACAGATCAA CAGGAACCTC ATCCAGAAGG CTGGTTACCT TAATCTTAGA 
AACAAAACAG GGCTGGTCAC CACCACCTGG GAGAGGCTTT ATTTCTTCAC CCAAGGCGGG 
AATCTCATGT GTCAGCCCAG GGGAGCCGTG GCTGGAGGTT TGATCCAGGA CCTGGACAAC 
TGCTCAGTGA TGGCCGTGGA TTGCGAAGAC CGGCGCTACT GCTTCCAGAT CACCACGCCC 
AATGGAAAAT CGGGAATAAT CCTCCAGGCT GAGAGCAGAA AGGAAAATGA AGAGTGGATA 
TGTGCAATAA ACAACATCTC CAGACAGATC TACCTGACCG ACAACCCTGA GGCAGTCGCG 
ATCAAGTTGA ATCAGACCGC TCTGCAAGCA GTGACTCCCA TTACAAGTTT TGGAAAAAAA 
CAAGAAAGCT CATGCCCCAG CCAGAACCTG AAAAATTCAG AGATGGAAAA TGAAAATGAC 
AAGATTGTTC CCAAAGCAAC AGCCAGTCTA CCTGAAGCAG AGGAGCTGAT CGCGCCTGGA 
ACGCCGATTC AATTCGATAT TGTGCTTCCT GCTACAGAAT TCCTTGATCA GAACAGAGGG 
AGCAGGCGTA CCAACCCTTT TGGTGAAACT GAGGATGAAT CATTTCCAGA AGCAGAAGAT 
TCTCTTTTGC AGCAGATGTT TATAGTTCGG TTTTTGGGAT CAATGGCAGT TAAAACAGAC 
AGCACTACTG AAGTGATTTA TGAAGCGATG AGACAAGTAT TGGCTGCTCG GGCTATTCAT 
AACATCTTCC GCATGACAGA ATCCCATCTG ATGGTCACCA GTCAATCTTT GAGGTTGATA 
GATCCACAGA CTCAAGTATC AAGGGCCAAT TTTGAACTTA CCAGTGTCAC ACAATTTGCT 
GCTCATCAAG AAAACAAGAG ACTGGTTGGT TTTGTCATCC GTGTTCCTGA ATCCACTGGA 
GAAGAATCTC TGAGTACATA CATTTTTGAA AGCAACTCAG AAGGCGAAAA GATATGTTAT 
GCTATTAATT TGGGAAAAGA AATTATTGAG GTTCAGAAGG ATCCAGAAGC ACTGGCTCAA 
TTAATGCTGT CCATACCACT AACCAATGAC GGAAAATATG TACTGTTAAA CGATCAACCA 
GATGACGATG ATGGAAATCC AAACGAACAT AGAGGCGCAG AATCCGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_011538530.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536832.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu69147
Clone ID Related Accession (Same CDS sequence)	XM_011538530.2 , XM_011538530.3
Accession Version	XM_011538530.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011538530.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACAC CCCAGACTCG CTCTTTACTG AGCGTGTTTG AAGAAGATGC TGGCACCCTC 
ACAGACTATA CCAACCAGCT GCTCCAGGCA ATGCAGCGCG TCTATGGAGC CCAGAATGAG 
ATGTGCCTGG CCACACAACA GCTTTCTAAG CAACTGCTGG CATATGAAAA ACAGAACTTT 
GCTCTTGGCA AAGGTGATGA AGAAGTAATT TCAACACTCC ACTATTTTTC CAAAGTGGTG 
GATGAGCTTA ATCTTCTCCA TACAGAGCTG GCTAAACAGT TGGCAGACAC AATGGTTCTA 
CCTATCATAC AATTCCGAGA AAAGGATCTC ACAGAAGTAA GCACTTTAAA GGATCTATTT 
GGACTCGCTA GCAATGATGT CTGTTTATTT TTAGAGCATG ACCTCTCAAT GGCAAAATAC 
AGCAGGCTGC CTAAGAAAAA GGAGAATGAG AAGGTGAAGA CCGAAGTCGG AAAAGAGGTG 
GCCGCGGCCC GGCGGAAGCA GCACCTCTCC TCCCTTCAGT ACTACTGTGC CCTCAACGCG 
CTGCAGTACA GAAAGCAAAT GGCCATGATG GAGCCCATGA TAGGCTTTGC CCATGGACAG 
ATTAACTTTT TTAAGAAGGG AGCAGAGATG TTTTCCAAAC GTATGGACAG CTTTTTATCC 
TCCGTTGCAG ACATGGTTCA AAGCATTCAG GTAGAACTGG AAGCCGAGGC GGAAAAGATG 
CGGGTGTCCC AGCAAGAATT ACTTTCTGTT GATGAATCTG TTTACACTCC AGACTCTGAT 
GTGGCCGCAC CACAGATCAA CAGGAACCTC ATCCAGAAGG CTGGTTACCT TAATCTTAGA 
AACAAAACAG GGCTGGTCAC CACCACCTGG GAGAGGCTTT ATTTCTTCAC CCAAGGCGGG 
AATCTCATGT GTCAGCCCAG GGGAGCCGTG GCTGGAGGTT TGATCCAGGA CCTGGACAAC 
TGCTCAGTGA TGGCCGTGGA TTGCGAAGAC CGGCGCTACT GCTTCCAGAT CACCACGCCC 
AATGGAAAAT CGGGAATAAT CCTCCAGGCT GAGAGCAGAA AGGAAAATGA AGAGTGGATA 
TGTGCAATAA ACAACATCTC CAGACAGATC TACCTGACCG ACAACCCTGA GGCAGTCGCG 
ATCAAGTTGA ATCAGACCGC TCTGCAAGCA GTGACTCCCA TTACAAGTTT TGGAAAAAAA 
CAAGAAAGCT CATGCCCCAG CCAGAACCTG AAAAATTCAG AGATGGAAAA TGAAAATGAC 
AAGATTGTTC CCAAAGCAAC AGCCAGTCTA CCTGAAGCAG AGGAGCTGAT CGCGCCTGGA 
ACGCCGATTC AATTCGATAT TGTGCTTCCT GCTACAGAAT TCCTTGATCA GAACAGAGGG 
AGCAGGCGTA CCAACCCTTT TGGTGAAACT GAGGATGAAT CATTTCCAGA AGCAGAAGAT 
TCTCTTTTGC AGCAGATGTT TATAGTTCGG TTTTTGGGAT CAATGGCAGT TAAAACAGAC 
AGCACTACTG AAGTGATTTA TGAAGCGATG AGACAAGTAT TGGCTGCTCG GGCTATTCAT 
AACATCTTCC GCATGACAGA ATCCCATCTG ATGGTCACCA GTCAATCTTT GAGGTTGATA 
GATCCACAGA CTCAAGTATC AAGGGCCAAT TTTGAACTTA CCAGTGTCAC ACAATTTGCT 
GCTCATCAAG AAAACAAGAG ACTGGTTGGT TTTGTCATCC GTGTTCCTGA ATCCACTGGA 
GAAGAATCTC TGAGTACATA CATTTTTGAA AGCAACTCAG AAGGCGAAAA GATATGTTAT 
GCTATTAATT TGGGAAAAGA AATTATTGAG GTTCAGAAGG ATCCAGAAGC ACTGGCTCAA 
TTAATGCTGT CCATACCACT AACCAATGAC GGAAAATATG TACTGTTAAA CGATCAACCA 
GATGACGATG ATGGAAATCC AAACGAACAT AGAGGCGCAG AATCCGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536832.1
CDS	1710..3683
Translation	MTTPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNDVCLFLEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	XM_011538530.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538530.3

ORF Insert Sequence:

ATGACCACAC CCCAGACTCG CTCTTTACTG AGCGTGTTTG AAGAAGATGC TGGCACCCTC 
ACAGACTATA CCAACCAGCT GCTCCAGGCA ATGCAGCGCG TCTATGGAGC CCAGAATGAG 
ATGTGCCTGG CCACACAACA GCTTTCTAAG CAACTGCTGG CATATGAAAA ACAGAACTTT 
GCTCTTGGCA AAGGTGATGA AGAAGTAATT TCAACACTCC ACTATTTTTC CAAAGTGGTG 
GATGAGCTTA ATCTTCTCCA TACAGAGCTG GCTAAACAGT TGGCAGACAC AATGGTTCTA 
CCTATCATAC AATTCCGAGA AAAGGATCTC ACAGAAGTAA GCACTTTAAA GGATCTATTT 
GGACTCGCTA GCAATGATGT CTGTTTATTT TTAGAGCATG ACCTCTCAAT GGCAAAATAC 
AGCAGGCTGC CTAAGAAAAA GGAGAATGAG AAGGTGAAGA CCGAAGTCGG AAAAGAGGTG 
GCCGCGGCCC GGCGGAAGCA GCACCTCTCC TCCCTTCAGT ACTACTGTGC CCTCAACGCG 
CTGCAGTACA GAAAGCAAAT GGCCATGATG GAGCCCATGA TAGGCTTTGC CCATGGACAG 
ATTAACTTTT TTAAGAAGGG AGCAGAGATG TTTTCCAAAC GTATGGACAG CTTTTTATCC 
TCCGTTGCAG ACATGGTTCA AAGCATTCAG GTAGAACTGG AAGCCGAGGC GGAAAAGATG 
CGGGTGTCCC AGCAAGAATT ACTTTCTGTT GATGAATCTG TTTACACTCC AGACTCTGAT 
GTGGCCGCAC CACAGATCAA CAGGAACCTC ATCCAGAAGG CTGGTTACCT TAATCTTAGA 
AACAAAACAG GGCTGGTCAC CACCACCTGG GAGAGGCTTT ATTTCTTCAC CCAAGGCGGG 
AATCTCATGT GTCAGCCCAG GGGAGCCGTG GCTGGAGGTT TGATCCAGGA CCTGGACAAC 
TGCTCAGTGA TGGCCGTGGA TTGCGAAGAC CGGCGCTACT GCTTCCAGAT CACCACGCCC 
AATGGAAAAT CGGGAATAAT CCTCCAGGCT GAGAGCAGAA AGGAAAATGA AGAGTGGATA 
TGTGCAATAA ACAACATCTC CAGACAGATC TACCTGACCG ACAACCCTGA GGCAGTCGCG 
ATCAAGTTGA ATCAGACCGC TCTGCAAGCA GTGACTCCCA TTACAAGTTT TGGAAAAAAA 
CAAGAAAGCT CATGCCCCAG CCAGAACCTG AAAAATTCAG AGATGGAAAA TGAAAATGAC 
AAGATTGTTC CCAAAGCAAC AGCCAGTCTA CCTGAAGCAG AGGAGCTGAT CGCGCCTGGA 
ACGCCGATTC AATTCGATAT TGTGCTTCCT GCTACAGAAT TCCTTGATCA GAACAGAGGG 
AGCAGGCGTA CCAACCCTTT TGGTGAAACT GAGGATGAAT CATTTCCAGA AGCAGAAGAT 
TCTCTTTTGC AGCAGATGTT TATAGTTCGG TTTTTGGGAT CAATGGCAGT TAAAACAGAC 
AGCACTACTG AAGTGATTTA TGAAGCGATG AGACAAGTAT TGGCTGCTCG GGCTATTCAT 
AACATCTTCC GCATGACAGA ATCCCATCTG ATGGTCACCA GTCAATCTTT GAGGTTGATA 
GATCCACAGA CTCAAGTATC AAGGGCCAAT TTTGAACTTA CCAGTGTCAC ACAATTTGCT 
GCTCATCAAG AAAACAAGAG ACTGGTTGGT TTTGTCATCC GTGTTCCTGA ATCCACTGGA 
GAAGAATCTC TGAGTACATA CATTTTTGAA AGCAACTCAG AAGGCGAAAA GATATGTTAT 
GCTATTAATT TGGGAAAAGA AATTATTGAG GTTCAGAAGG ATCCAGAAGC ACTGGCTCAA 
TTAATGCTGT CCATACCACT AACCAATGAC GGAAAATATG TACTGTTAAA CGATCAACCA 
GATGACGATG ATGGAAATCC AAACGAACAT AGAGGCGCAG AATCCGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_011538531.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536833.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu69148
Clone ID Related Accession (Same CDS sequence)	XM_011538531.3 , XM_011538531.2 , XM_011538532.2 , XM_011538532.3
Accession Version	XM_011538531.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011538531.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGATGT CTGTTTATTT 
TTAGAGCATG ACCTCTCAAT GGCAAAATAC AGCAGGCTGC CTAAGAAAAA GGAGAATGAG 
AAGGTGAAGA CCGAAGTCGG AAAAGAGGTG GCCGCGGCCC GGCGGAAGCA GCACCTCTCC 
TCCCTTCAGT ACTACTGTGC CCTCAACGCG CTGCAGTACA GAAAGCAAAT GGCCATGATG 
GAGCCCATGA TAGGCTTTGC CCATGGACAG ATTAACTTTT TTAAGAAGGG AGCAGAGATG 
TTTTCCAAAC GTATGGACAG CTTTTTATCC TCCGTTGCAG ACATGGTTCA AAGCATTCAG 
GTAGAACTGG AAGCCGAGGC GGAAAAGATG CGGGTGTCCC AGCAAGAATT ACTTTCTGTT 
GATGAATCTG TTTACACTCC AGACTCTGAT GTGGCCGCAC CACAGATCAA CAGGAACCTC 
ATCCAGAAGG CTGGTTACCT TAATCTTAGA AACAAAACAG GGCTGGTCAC CACCACCTGG 
GAGAGGCTTT ATTTCTTCAC CCAAGGCGGG AATCTCATGT GTCAGCCCAG GGGAGCCGTG 
GCTGGAGGTT TGATCCAGGA CCTGGACAAC TGCTCAGTGA TGGCCGTGGA TTGCGAAGAC 
CGGCGCTACT GCTTCCAGAT CACCACGCCC AATGGAAAAT CGGGAATAAT CCTCCAGGCT 
GAGAGCAGAA AGGAAAATGA AGAGTGGATA TGTGCAATAA ACAACATCTC CAGACAGATC 
TACCTGACCG ACAACCCTGA GGCAGTCGCG ATCAAGTTGA ATCAGACCGC TCTGCAAGCA 
GTGACTCCCA TTACAAGTTT TGGAAAAAAA CAAGAAAGCT CATGCCCCAG CCAGAACCTG 
AAAAATTCAG AGATGGAAAA TGAAAATGAC AAGATTGTTC CCAAAGCAAC AGCCAGTCTA 
CCTGAAGCAG AGGAGCTGAT CGCGCCTGGA ACGCCGATTC AATTCGATAT TGTGCTTCCT 
GCTACAGAAT TCCTTGATCA GAACAGAGGG AGCAGGCGTA CCAACCCTTT TGGTGAAACT 
GAGGATGAAT CATTTCCAGA AGCAGAAGAT TCTCTTTTGC AGCAGATGTT TATAGTTCGG 
TTTTTGGGAT CAATGGCAGT TAAAACAGAC AGCACTACTG AAGTGATTTA TGAAGCGATG 
AGACAAGTAT TGGCTGCTCG GGCTATTCAT AACATCTTCC GCATGACAGA ATCCCATCTG 
ATGGTCACCA GTCAATCTTT GAGGTTGATA GATCCACAGA CTCAAGTATC AAGGGCCAAT 
TTTGAACTTA CCAGTGTCAC ACAATTTGCT GCTCATCAAG AAAACAAGAG ACTGGTTGGT 
TTTGTCATCC GTGTTCCTGA ATCCACTGGA GAAGAATCTC TGAGTACATA CATTTTTGAA 
AGCAACTCAG AAGGCGAAAA GATATGTTAT GCTATTAATT TGGGAAAAGA AATTATTGAG 
GTTCAGAAGG ATCCAGAAGC ACTGGCTCAA TTAATGCTGT CCATACCACT AACCAATGAC 
GGAAAATATG TACTGTTAAA CGATCAACCA GATGACGATG ATGGAAATCC AAACGAACAT 
AGAGGCGCAG AATCCGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536833.1
CDS	1279..3162
Translation	MQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNDVCLFLEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	XM_011538531.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538531.3

ORF Insert Sequence:

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGATGT CTGTTTATTT 
TTAGAGCATG ACCTCTCAAT GGCAAAATAC AGCAGGCTGC CTAAGAAAAA GGAGAATGAG 
AAGGTGAAGA CCGAAGTCGG AAAAGAGGTG GCCGCGGCCC GGCGGAAGCA GCACCTCTCC 
TCCCTTCAGT ACTACTGTGC CCTCAACGCG CTGCAGTACA GAAAGCAAAT GGCCATGATG 
GAGCCCATGA TAGGCTTTGC CCATGGACAG ATTAACTTTT TTAAGAAGGG AGCAGAGATG 
TTTTCCAAAC GTATGGACAG CTTTTTATCC TCCGTTGCAG ACATGGTTCA AAGCATTCAG 
GTAGAACTGG AAGCCGAGGC GGAAAAGATG CGGGTGTCCC AGCAAGAATT ACTTTCTGTT 
GATGAATCTG TTTACACTCC AGACTCTGAT GTGGCCGCAC CACAGATCAA CAGGAACCTC 
ATCCAGAAGG CTGGTTACCT TAATCTTAGA AACAAAACAG GGCTGGTCAC CACCACCTGG 
GAGAGGCTTT ATTTCTTCAC CCAAGGCGGG AATCTCATGT GTCAGCCCAG GGGAGCCGTG 
GCTGGAGGTT TGATCCAGGA CCTGGACAAC TGCTCAGTGA TGGCCGTGGA TTGCGAAGAC 
CGGCGCTACT GCTTCCAGAT CACCACGCCC AATGGAAAAT CGGGAATAAT CCTCCAGGCT 
GAGAGCAGAA AGGAAAATGA AGAGTGGATA TGTGCAATAA ACAACATCTC CAGACAGATC 
TACCTGACCG ACAACCCTGA GGCAGTCGCG ATCAAGTTGA ATCAGACCGC TCTGCAAGCA 
GTGACTCCCA TTACAAGTTT TGGAAAAAAA CAAGAAAGCT CATGCCCCAG CCAGAACCTG 
AAAAATTCAG AGATGGAAAA TGAAAATGAC AAGATTGTTC CCAAAGCAAC AGCCAGTCTA 
CCTGAAGCAG AGGAGCTGAT CGCGCCTGGA ACGCCGATTC AATTCGATAT TGTGCTTCCT 
GCTACAGAAT TCCTTGATCA GAACAGAGGG AGCAGGCGTA CCAACCCTTT TGGTGAAACT 
GAGGATGAAT CATTTCCAGA AGCAGAAGAT TCTCTTTTGC AGCAGATGTT TATAGTTCGG 
TTTTTGGGAT CAATGGCAGT TAAAACAGAC AGCACTACTG AAGTGATTTA TGAAGCGATG 
AGACAAGTAT TGGCTGCTCG GGCTATTCAT AACATCTTCC GCATGACAGA ATCCCATCTG 
ATGGTCACCA GTCAATCTTT GAGGTTGATA GATCCACAGA CTCAAGTATC AAGGGCCAAT 
TTTGAACTTA CCAGTGTCAC ACAATTTGCT GCTCATCAAG AAAACAAGAG ACTGGTTGGT 
TTTGTCATCC GTGTTCCTGA ATCCACTGGA GAAGAATCTC TGAGTACATA CATTTTTGAA 
AGCAACTCAG AAGGCGAAAA GATATGTTAT GCTATTAATT TGGGAAAAGA AATTATTGAG 
GTTCAGAAGG ATCCAGAAGC ACTGGCTCAA TTAATGCTGT CCATACCACT AACCAATGAC 
GGAAAATATG TACTGTTAAA CGATCAACCA GATGACGATG ATGGAAATCC AAACGAACAT 
AGAGGCGCAG AATCCGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_011538531.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536833.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu69148
Clone ID Related Accession (Same CDS sequence)	XM_011538531.3 , XM_011538531.2 , XM_011538532.2 , XM_011538532.3
Accession Version	XM_011538531.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538531.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGATGT CTGTTTATTT 
TTAGAGCATG ACCTCTCAAT GGCAAAATAC AGCAGGCTGC CTAAGAAAAA GGAGAATGAG 
AAGGTGAAGA CCGAAGTCGG AAAAGAGGTG GCCGCGGCCC GGCGGAAGCA GCACCTCTCC 
TCCCTTCAGT ACTACTGTGC CCTCAACGCG CTGCAGTACA GAAAGCAAAT GGCCATGATG 
GAGCCCATGA TAGGCTTTGC CCATGGACAG ATTAACTTTT TTAAGAAGGG AGCAGAGATG 
TTTTCCAAAC GTATGGACAG CTTTTTATCC TCCGTTGCAG ACATGGTTCA AAGCATTCAG 
GTAGAACTGG AAGCCGAGGC GGAAAAGATG CGGGTGTCCC AGCAAGAATT ACTTTCTGTT 
GATGAATCTG TTTACACTCC AGACTCTGAT GTGGCCGCAC CACAGATCAA CAGGAACCTC 
ATCCAGAAGG CTGGTTACCT TAATCTTAGA AACAAAACAG GGCTGGTCAC CACCACCTGG 
GAGAGGCTTT ATTTCTTCAC CCAAGGCGGG AATCTCATGT GTCAGCCCAG GGGAGCCGTG 
GCTGGAGGTT TGATCCAGGA CCTGGACAAC TGCTCAGTGA TGGCCGTGGA TTGCGAAGAC 
CGGCGCTACT GCTTCCAGAT CACCACGCCC AATGGAAAAT CGGGAATAAT CCTCCAGGCT 
GAGAGCAGAA AGGAAAATGA AGAGTGGATA TGTGCAATAA ACAACATCTC CAGACAGATC 
TACCTGACCG ACAACCCTGA GGCAGTCGCG ATCAAGTTGA ATCAGACCGC TCTGCAAGCA 
GTGACTCCCA TTACAAGTTT TGGAAAAAAA CAAGAAAGCT CATGCCCCAG CCAGAACCTG 
AAAAATTCAG AGATGGAAAA TGAAAATGAC AAGATTGTTC CCAAAGCAAC AGCCAGTCTA 
CCTGAAGCAG AGGAGCTGAT CGCGCCTGGA ACGCCGATTC AATTCGATAT TGTGCTTCCT 
GCTACAGAAT TCCTTGATCA GAACAGAGGG AGCAGGCGTA CCAACCCTTT TGGTGAAACT 
GAGGATGAAT CATTTCCAGA AGCAGAAGAT TCTCTTTTGC AGCAGATGTT TATAGTTCGG 
TTTTTGGGAT CAATGGCAGT TAAAACAGAC AGCACTACTG AAGTGATTTA TGAAGCGATG 
AGACAAGTAT TGGCTGCTCG GGCTATTCAT AACATCTTCC GCATGACAGA ATCCCATCTG 
ATGGTCACCA GTCAATCTTT GAGGTTGATA GATCCACAGA CTCAAGTATC AAGGGCCAAT 
TTTGAACTTA CCAGTGTCAC ACAATTTGCT GCTCATCAAG AAAACAAGAG ACTGGTTGGT 
TTTGTCATCC GTGTTCCTGA ATCCACTGGA GAAGAATCTC TGAGTACATA CATTTTTGAA 
AGCAACTCAG AAGGCGAAAA GATATGTTAT GCTATTAATT TGGGAAAAGA AATTATTGAG 
GTTCAGAAGG ATCCAGAAGC ACTGGCTCAA TTAATGCTGT CCATACCACT AACCAATGAC 
GGAAAATATG TACTGTTAAA CGATCAACCA GATGACGATG ATGGAAATCC AAACGAACAT 
AGAGGCGCAG AATCCGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536833.1
CDS	1463..3346
Translation	MQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNDVCLFLEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	XM_011538531.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538531.2

ORF Insert Sequence:

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGATGT CTGTTTATTT 
TTAGAGCATG ACCTCTCAAT GGCAAAATAC AGCAGGCTGC CTAAGAAAAA GGAGAATGAG 
AAGGTGAAGA CCGAAGTCGG AAAAGAGGTG GCCGCGGCCC GGCGGAAGCA GCACCTCTCC 
TCCCTTCAGT ACTACTGTGC CCTCAACGCG CTGCAGTACA GAAAGCAAAT GGCCATGATG 
GAGCCCATGA TAGGCTTTGC CCATGGACAG ATTAACTTTT TTAAGAAGGG AGCAGAGATG 
TTTTCCAAAC GTATGGACAG CTTTTTATCC TCCGTTGCAG ACATGGTTCA AAGCATTCAG 
GTAGAACTGG AAGCCGAGGC GGAAAAGATG CGGGTGTCCC AGCAAGAATT ACTTTCTGTT 
GATGAATCTG TTTACACTCC AGACTCTGAT GTGGCCGCAC CACAGATCAA CAGGAACCTC 
ATCCAGAAGG CTGGTTACCT TAATCTTAGA AACAAAACAG GGCTGGTCAC CACCACCTGG 
GAGAGGCTTT ATTTCTTCAC CCAAGGCGGG AATCTCATGT GTCAGCCCAG GGGAGCCGTG 
GCTGGAGGTT TGATCCAGGA CCTGGACAAC TGCTCAGTGA TGGCCGTGGA TTGCGAAGAC 
CGGCGCTACT GCTTCCAGAT CACCACGCCC AATGGAAAAT CGGGAATAAT CCTCCAGGCT 
GAGAGCAGAA AGGAAAATGA AGAGTGGATA TGTGCAATAA ACAACATCTC CAGACAGATC 
TACCTGACCG ACAACCCTGA GGCAGTCGCG ATCAAGTTGA ATCAGACCGC TCTGCAAGCA 
GTGACTCCCA TTACAAGTTT TGGAAAAAAA CAAGAAAGCT CATGCCCCAG CCAGAACCTG 
AAAAATTCAG AGATGGAAAA TGAAAATGAC AAGATTGTTC CCAAAGCAAC AGCCAGTCTA 
CCTGAAGCAG AGGAGCTGAT CGCGCCTGGA ACGCCGATTC AATTCGATAT TGTGCTTCCT 
GCTACAGAAT TCCTTGATCA GAACAGAGGG AGCAGGCGTA CCAACCCTTT TGGTGAAACT 
GAGGATGAAT CATTTCCAGA AGCAGAAGAT TCTCTTTTGC AGCAGATGTT TATAGTTCGG 
TTTTTGGGAT CAATGGCAGT TAAAACAGAC AGCACTACTG AAGTGATTTA TGAAGCGATG 
AGACAAGTAT TGGCTGCTCG GGCTATTCAT AACATCTTCC GCATGACAGA ATCCCATCTG 
ATGGTCACCA GTCAATCTTT GAGGTTGATA GATCCACAGA CTCAAGTATC AAGGGCCAAT 
TTTGAACTTA CCAGTGTCAC ACAATTTGCT GCTCATCAAG AAAACAAGAG ACTGGTTGGT 
TTTGTCATCC GTGTTCCTGA ATCCACTGGA GAAGAATCTC TGAGTACATA CATTTTTGAA 
AGCAACTCAG AAGGCGAAAA GATATGTTAT GCTATTAATT TGGGAAAAGA AATTATTGAG 
GTTCAGAAGG ATCCAGAAGC ACTGGCTCAA TTAATGCTGT CCATACCACT AACCAATGAC 
GGAAAATATG TACTGTTAAA CGATCAACCA GATGACGATG ATGGAAATCC AAACGAACAT 
AGAGGCGCAG AATCCGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_011538532.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536834.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu69148
Clone ID Related Accession (Same CDS sequence)	XM_011538531.3 , XM_011538531.2 , XM_011538532.2 , XM_011538532.3
Accession Version	XM_011538532.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGATGT CTGTTTATTT 
TTAGAGCATG ACCTCTCAAT GGCAAAATAC AGCAGGCTGC CTAAGAAAAA GGAGAATGAG 
AAGGTGAAGA CCGAAGTCGG AAAAGAGGTG GCCGCGGCCC GGCGGAAGCA GCACCTCTCC 
TCCCTTCAGT ACTACTGTGC CCTCAACGCG CTGCAGTACA GAAAGCAAAT GGCCATGATG 
GAGCCCATGA TAGGCTTTGC CCATGGACAG ATTAACTTTT TTAAGAAGGG AGCAGAGATG 
TTTTCCAAAC GTATGGACAG CTTTTTATCC TCCGTTGCAG ACATGGTTCA AAGCATTCAG 
GTAGAACTGG AAGCCGAGGC GGAAAAGATG CGGGTGTCCC AGCAAGAATT ACTTTCTGTT 
GATGAATCTG TTTACACTCC AGACTCTGAT GTGGCCGCAC CACAGATCAA CAGGAACCTC 
ATCCAGAAGG CTGGTTACCT TAATCTTAGA AACAAAACAG GGCTGGTCAC CACCACCTGG 
GAGAGGCTTT ATTTCTTCAC CCAAGGCGGG AATCTCATGT GTCAGCCCAG GGGAGCCGTG 
GCTGGAGGTT TGATCCAGGA CCTGGACAAC TGCTCAGTGA TGGCCGTGGA TTGCGAAGAC 
CGGCGCTACT GCTTCCAGAT CACCACGCCC AATGGAAAAT CGGGAATAAT CCTCCAGGCT 
GAGAGCAGAA AGGAAAATGA AGAGTGGATA TGTGCAATAA ACAACATCTC CAGACAGATC 
TACCTGACCG ACAACCCTGA GGCAGTCGCG ATCAAGTTGA ATCAGACCGC TCTGCAAGCA 
GTGACTCCCA TTACAAGTTT TGGAAAAAAA CAAGAAAGCT CATGCCCCAG CCAGAACCTG 
AAAAATTCAG AGATGGAAAA TGAAAATGAC AAGATTGTTC CCAAAGCAAC AGCCAGTCTA 
CCTGAAGCAG AGGAGCTGAT CGCGCCTGGA ACGCCGATTC AATTCGATAT TGTGCTTCCT 
GCTACAGAAT TCCTTGATCA GAACAGAGGG AGCAGGCGTA CCAACCCTTT TGGTGAAACT 
GAGGATGAAT CATTTCCAGA AGCAGAAGAT TCTCTTTTGC AGCAGATGTT TATAGTTCGG 
TTTTTGGGAT CAATGGCAGT TAAAACAGAC AGCACTACTG AAGTGATTTA TGAAGCGATG 
AGACAAGTAT TGGCTGCTCG GGCTATTCAT AACATCTTCC GCATGACAGA ATCCCATCTG 
ATGGTCACCA GTCAATCTTT GAGGTTGATA GATCCACAGA CTCAAGTATC AAGGGCCAAT 
TTTGAACTTA CCAGTGTCAC ACAATTTGCT GCTCATCAAG AAAACAAGAG ACTGGTTGGT 
TTTGTCATCC GTGTTCCTGA ATCCACTGGA GAAGAATCTC TGAGTACATA CATTTTTGAA 
AGCAACTCAG AAGGCGAAAA GATATGTTAT GCTATTAATT TGGGAAAAGA AATTATTGAG 
GTTCAGAAGG ATCCAGAAGC ACTGGCTCAA TTAATGCTGT CCATACCACT AACCAATGAC 
GGAAAATATG TACTGTTAAA CGATCAACCA GATGACGATG ATGGAAATCC AAACGAACAT 
AGAGGCGCAG AATCCGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536834.1
CDS	2789..4672
Translation	MQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNDVCLFLEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	XM_011538532.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538532.2

ORF Insert Sequence:

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGATGT CTGTTTATTT 
TTAGAGCATG ACCTCTCAAT GGCAAAATAC AGCAGGCTGC CTAAGAAAAA GGAGAATGAG 
AAGGTGAAGA CCGAAGTCGG AAAAGAGGTG GCCGCGGCCC GGCGGAAGCA GCACCTCTCC 
TCCCTTCAGT ACTACTGTGC CCTCAACGCG CTGCAGTACA GAAAGCAAAT GGCCATGATG 
GAGCCCATGA TAGGCTTTGC CCATGGACAG ATTAACTTTT TTAAGAAGGG AGCAGAGATG 
TTTTCCAAAC GTATGGACAG CTTTTTATCC TCCGTTGCAG ACATGGTTCA AAGCATTCAG 
GTAGAACTGG AAGCCGAGGC GGAAAAGATG CGGGTGTCCC AGCAAGAATT ACTTTCTGTT 
GATGAATCTG TTTACACTCC AGACTCTGAT GTGGCCGCAC CACAGATCAA CAGGAACCTC 
ATCCAGAAGG CTGGTTACCT TAATCTTAGA AACAAAACAG GGCTGGTCAC CACCACCTGG 
GAGAGGCTTT ATTTCTTCAC CCAAGGCGGG AATCTCATGT GTCAGCCCAG GGGAGCCGTG 
GCTGGAGGTT TGATCCAGGA CCTGGACAAC TGCTCAGTGA TGGCCGTGGA TTGCGAAGAC 
CGGCGCTACT GCTTCCAGAT CACCACGCCC AATGGAAAAT CGGGAATAAT CCTCCAGGCT 
GAGAGCAGAA AGGAAAATGA AGAGTGGATA TGTGCAATAA ACAACATCTC CAGACAGATC 
TACCTGACCG ACAACCCTGA GGCAGTCGCG ATCAAGTTGA ATCAGACCGC TCTGCAAGCA 
GTGACTCCCA TTACAAGTTT TGGAAAAAAA CAAGAAAGCT CATGCCCCAG CCAGAACCTG 
AAAAATTCAG AGATGGAAAA TGAAAATGAC AAGATTGTTC CCAAAGCAAC AGCCAGTCTA 
CCTGAAGCAG AGGAGCTGAT CGCGCCTGGA ACGCCGATTC AATTCGATAT TGTGCTTCCT 
GCTACAGAAT TCCTTGATCA GAACAGAGGG AGCAGGCGTA CCAACCCTTT TGGTGAAACT 
GAGGATGAAT CATTTCCAGA AGCAGAAGAT TCTCTTTTGC AGCAGATGTT TATAGTTCGG 
TTTTTGGGAT CAATGGCAGT TAAAACAGAC AGCACTACTG AAGTGATTTA TGAAGCGATG 
AGACAAGTAT TGGCTGCTCG GGCTATTCAT AACATCTTCC GCATGACAGA ATCCCATCTG 
ATGGTCACCA GTCAATCTTT GAGGTTGATA GATCCACAGA CTCAAGTATC AAGGGCCAAT 
TTTGAACTTA CCAGTGTCAC ACAATTTGCT GCTCATCAAG AAAACAAGAG ACTGGTTGGT 
TTTGTCATCC GTGTTCCTGA ATCCACTGGA GAAGAATCTC TGAGTACATA CATTTTTGAA 
AGCAACTCAG AAGGCGAAAA GATATGTTAT GCTATTAATT TGGGAAAAGA AATTATTGAG 
GTTCAGAAGG ATCCAGAAGC ACTGGCTCAA TTAATGCTGT CCATACCACT AACCAATGAC 
GGAAAATATG TACTGTTAAA CGATCAACCA GATGACGATG ATGGAAATCC AAACGAACAT 
AGAGGCGCAG AATCCGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_011538532.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536834.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu69148
Clone ID Related Accession (Same CDS sequence)	XM_011538531.3 , XM_011538531.2 , XM_011538532.2 , XM_011538532.3
Accession Version	XM_011538532.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011538532.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGATGT CTGTTTATTT 
TTAGAGCATG ACCTCTCAAT GGCAAAATAC AGCAGGCTGC CTAAGAAAAA GGAGAATGAG 
AAGGTGAAGA CCGAAGTCGG AAAAGAGGTG GCCGCGGCCC GGCGGAAGCA GCACCTCTCC 
TCCCTTCAGT ACTACTGTGC CCTCAACGCG CTGCAGTACA GAAAGCAAAT GGCCATGATG 
GAGCCCATGA TAGGCTTTGC CCATGGACAG ATTAACTTTT TTAAGAAGGG AGCAGAGATG 
TTTTCCAAAC GTATGGACAG CTTTTTATCC TCCGTTGCAG ACATGGTTCA AAGCATTCAG 
GTAGAACTGG AAGCCGAGGC GGAAAAGATG CGGGTGTCCC AGCAAGAATT ACTTTCTGTT 
GATGAATCTG TTTACACTCC AGACTCTGAT GTGGCCGCAC CACAGATCAA CAGGAACCTC 
ATCCAGAAGG CTGGTTACCT TAATCTTAGA AACAAAACAG GGCTGGTCAC CACCACCTGG 
GAGAGGCTTT ATTTCTTCAC CCAAGGCGGG AATCTCATGT GTCAGCCCAG GGGAGCCGTG 
GCTGGAGGTT TGATCCAGGA CCTGGACAAC TGCTCAGTGA TGGCCGTGGA TTGCGAAGAC 
CGGCGCTACT GCTTCCAGAT CACCACGCCC AATGGAAAAT CGGGAATAAT CCTCCAGGCT 
GAGAGCAGAA AGGAAAATGA AGAGTGGATA TGTGCAATAA ACAACATCTC CAGACAGATC 
TACCTGACCG ACAACCCTGA GGCAGTCGCG ATCAAGTTGA ATCAGACCGC TCTGCAAGCA 
GTGACTCCCA TTACAAGTTT TGGAAAAAAA CAAGAAAGCT CATGCCCCAG CCAGAACCTG 
AAAAATTCAG AGATGGAAAA TGAAAATGAC AAGATTGTTC CCAAAGCAAC AGCCAGTCTA 
CCTGAAGCAG AGGAGCTGAT CGCGCCTGGA ACGCCGATTC AATTCGATAT TGTGCTTCCT 
GCTACAGAAT TCCTTGATCA GAACAGAGGG AGCAGGCGTA CCAACCCTTT TGGTGAAACT 
GAGGATGAAT CATTTCCAGA AGCAGAAGAT TCTCTTTTGC AGCAGATGTT TATAGTTCGG 
TTTTTGGGAT CAATGGCAGT TAAAACAGAC AGCACTACTG AAGTGATTTA TGAAGCGATG 
AGACAAGTAT TGGCTGCTCG GGCTATTCAT AACATCTTCC GCATGACAGA ATCCCATCTG 
ATGGTCACCA GTCAATCTTT GAGGTTGATA GATCCACAGA CTCAAGTATC AAGGGCCAAT 
TTTGAACTTA CCAGTGTCAC ACAATTTGCT GCTCATCAAG AAAACAAGAG ACTGGTTGGT 
TTTGTCATCC GTGTTCCTGA ATCCACTGGA GAAGAATCTC TGAGTACATA CATTTTTGAA 
AGCAACTCAG AAGGCGAAAA GATATGTTAT GCTATTAATT TGGGAAAAGA AATTATTGAG 
GTTCAGAAGG ATCCAGAAGC ACTGGCTCAA TTAATGCTGT CCATACCACT AACCAATGAC 
GGAAAATATG TACTGTTAAA CGATCAACCA GATGACGATG ATGGAAATCC AAACGAACAT 
AGAGGCGCAG AATCCGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536834.1
CDS	2789..4672
Translation	MQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNDVCLFLEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	XM_011538532.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538532.3

ORF Insert Sequence:

ATGCAGCGCG TCTATGGAGC CCAGAATGAG ATGTGCCTGG CCACACAACA GCTTTCTAAG 
CAACTGCTGG CATATGAAAA ACAGAACTTT GCTCTTGGCA AAGGTGATGA AGAAGTAATT 
TCAACACTCC ACTATTTTTC CAAAGTGGTG GATGAGCTTA ATCTTCTCCA TACAGAGCTG 
GCTAAACAGT TGGCAGACAC AATGGTTCTA CCTATCATAC AATTCCGAGA AAAGGATCTC 
ACAGAAGTAA GCACTTTAAA GGATCTATTT GGACTCGCTA GCAATGATGT CTGTTTATTT 
TTAGAGCATG ACCTCTCAAT GGCAAAATAC AGCAGGCTGC CTAAGAAAAA GGAGAATGAG 
AAGGTGAAGA CCGAAGTCGG AAAAGAGGTG GCCGCGGCCC GGCGGAAGCA GCACCTCTCC 
TCCCTTCAGT ACTACTGTGC CCTCAACGCG CTGCAGTACA GAAAGCAAAT GGCCATGATG 
GAGCCCATGA TAGGCTTTGC CCATGGACAG ATTAACTTTT TTAAGAAGGG AGCAGAGATG 
TTTTCCAAAC GTATGGACAG CTTTTTATCC TCCGTTGCAG ACATGGTTCA AAGCATTCAG 
GTAGAACTGG AAGCCGAGGC GGAAAAGATG CGGGTGTCCC AGCAAGAATT ACTTTCTGTT 
GATGAATCTG TTTACACTCC AGACTCTGAT GTGGCCGCAC CACAGATCAA CAGGAACCTC 
ATCCAGAAGG CTGGTTACCT TAATCTTAGA AACAAAACAG GGCTGGTCAC CACCACCTGG 
GAGAGGCTTT ATTTCTTCAC CCAAGGCGGG AATCTCATGT GTCAGCCCAG GGGAGCCGTG 
GCTGGAGGTT TGATCCAGGA CCTGGACAAC TGCTCAGTGA TGGCCGTGGA TTGCGAAGAC 
CGGCGCTACT GCTTCCAGAT CACCACGCCC AATGGAAAAT CGGGAATAAT CCTCCAGGCT 
GAGAGCAGAA AGGAAAATGA AGAGTGGATA TGTGCAATAA ACAACATCTC CAGACAGATC 
TACCTGACCG ACAACCCTGA GGCAGTCGCG ATCAAGTTGA ATCAGACCGC TCTGCAAGCA 
GTGACTCCCA TTACAAGTTT TGGAAAAAAA CAAGAAAGCT CATGCCCCAG CCAGAACCTG 
AAAAATTCAG AGATGGAAAA TGAAAATGAC AAGATTGTTC CCAAAGCAAC AGCCAGTCTA 
CCTGAAGCAG AGGAGCTGAT CGCGCCTGGA ACGCCGATTC AATTCGATAT TGTGCTTCCT 
GCTACAGAAT TCCTTGATCA GAACAGAGGG AGCAGGCGTA CCAACCCTTT TGGTGAAACT 
GAGGATGAAT CATTTCCAGA AGCAGAAGAT TCTCTTTTGC AGCAGATGTT TATAGTTCGG 
TTTTTGGGAT CAATGGCAGT TAAAACAGAC AGCACTACTG AAGTGATTTA TGAAGCGATG 
AGACAAGTAT TGGCTGCTCG GGCTATTCAT AACATCTTCC GCATGACAGA ATCCCATCTG 
ATGGTCACCA GTCAATCTTT GAGGTTGATA GATCCACAGA CTCAAGTATC AAGGGCCAAT 
TTTGAACTTA CCAGTGTCAC ACAATTTGCT GCTCATCAAG AAAACAAGAG ACTGGTTGGT 
TTTGTCATCC GTGTTCCTGA ATCCACTGGA GAAGAATCTC TGAGTACATA CATTTTTGAA 
AGCAACTCAG AAGGCGAAAA GATATGTTAT GCTATTAATT TGGGAAAAGA AATTATTGAG 
GTTCAGAAGG ATCCAGAAGC ACTGGCTCAA TTAATGCTGT CCATACCACT AACCAATGAC 
GGAAAATATG TACTGTTAAA CGATCAACCA GATGACGATG ATGGAAATCC AAACGAACAT 
AGAGGCGCAG AATCCGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( NM_001251904.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001238833.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15838
Clone ID Related Accession (Same CDS sequence)	NM_001251904.1
Accession Version	NM_001251904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY113704.1, AK297100.1 and BC028008.1. Transcript Variant: This variant (2) has an additional in-frame segment in the coding region, compared to variant 1. The resulting isoform 2 is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.1622.1, SRR1803613.31365.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGCCG TGGACAAGCT CCTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GCGTGTTTGA AGAAGATGCT GGCACCCTCA CAGACTATAC CAACCAGCTG 
CTCCAGGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAGA TGTGCCTGGC CACACAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAACTTTG CTCTTGGCAA AGGTGATGAA 
GAAGTAATTT CAACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TCTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CTATCATACA ATTCCGAGAA 
AAGGATCTCA CAGAAGTAAG CACTTTAAAG GATCTATTTG GACTCGCTAG CAATGATGTC 
TGTTTATTTT TAGAGCATGA CCTCTCAATG GCAAAATACA GCAGGCTGCC TAAGAAAAAG 
GAGAATGAGA AGGTGAAGAC CGAAGTCGGA AAAGAGGTGG CCGCGGCCCG GCGGAAGCAG 
CACCTCTCCT CCCTTCAGTA CTACTGTGCC CTCAACGCGC TGCAGTACAG AAAGCAAATG 
GCCATGATGG AGCCCATGAT AGGCTTTGCC CATGGACAGA TTAACTTTTT TAAGAAGGGA 
GCAGAGATGT TTTCCAAACG TATGGACAGC TTTTTATCCT CCGTTGCAGA CATGGTTCAA 
AGCATTCAGG TAGAACTGGA AGCCGAGGCG GAAAAGATGC GGGTGTCCCA GCAAGAATTA 
CTTTCTGTTG ATGAATCTGT TTACACTCCA GACTCTGATG TGGCCGCACC ACAGATCAAC 
AGGAACCTCA TCCAGAAGGC TGGTTACCTT AATCTTAGAA ACAAAACAGG GCTGGTCACC 
ACCACCTGGG AGAGGCTTTA TTTCTTCACC CAAGGCGGGA ATCTCATGTG TCAGCCCAGG 
GGAGCCGTGG CTGGAGGTTT GATCCAGGAC CTGGACAACT GCTCAGTGAT GGCCGTGGAT 
TGCGAAGACC GGCGCTACTG CTTCCAGATC ACCACGCCCA ATGGAAAATC GGGAATAATC 
CTCCAGGCTG AGAGCAGAAA GGAAAATGAA GAGTGGATAT GTGCAATAAA CAACATCTCC 
AGACAGATCT ACCTGACCGA CAACCCTGAG GCAGTCGCGA TCAAGTTGAA TCAGACCGCT 
CTGCAAGCAG TGACTCCCAT TACAAGTTTT GGAAAAAAAC AAGAAAGCTC ATGCCCCAGC 
CAGAACCTGA AAAATTCAGA GATGGAAAAT GAAAATGACA AGATTGTTCC CAAAGCAACA 
GCCAGTCTAC CTGAAGCAGA GGAGCTGATC GCGCCTGGAA CGCCGATTCA ATTCGATATT 
GTGCTTCCTG CTACAGAATT CCTTGATCAG AACAGAGGGA GCAGGCGTAC CAACCCTTTT 
GGTGAAACTG AGGATGAATC ATTTCCAGAA GCAGAAGATT CTCTTTTGCA GCAGATGTTT 
ATAGTTCGGT TTTTGGGATC AATGGCAGTT AAAACAGACA GCACTACTGA AGTGATTTAT 
GAAGCGATGA GACAAGTATT GGCTGCTCGG GCTATTCATA ACATCTTCCG CATGACAGAA 
TCCCATCTGA TGGTCACCAG TCAATCTTTG AGGTTGATAG ATCCACAGAC TCAAGTATCA 
AGGGCCAATT TTGAACTTAC CAGTGTCACA CAATTTGCTG CTCATCAAGA AAACAAGAGA 
CTGGTTGGTT TTGTCATCCG TGTTCCTGAA TCCACTGGAG AAGAATCTCT GAGTACATAC 
ATTTTTGAAA GCAACTCAGA AGGCGAAAAG ATATGTTATG CTATTAATTT GGGAAAAGAA 
ATTATTGAGG TTCAGAAGGA TCCAGAAGCA CTGGCTCAAT TAATGCTGTC CATACCACTA 
ACCAATGACG GAAAATATGT ACTGTTAAAC GATCAACCAG ATGACGATGA TGGAAATCCA 
AACGAACATA GAGGCGCAGA ATCCGAAGCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238833.1
CDS	219..2231
Translation	MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNDVCLFLEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	NM_001251904.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001251904.1

ORF Insert Sequence:

ATGCCCGCCG TGGACAAGCT CCTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GCGTGTTTGA AGAAGATGCT GGCACCCTCA CAGACTATAC CAACCAGCTG 
CTCCAGGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAGA TGTGCCTGGC CACACAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAACTTTG CTCTTGGCAA AGGTGATGAA 
GAAGTAATTT CAACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TCTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CTATCATACA ATTCCGAGAA 
AAGGATCTCA CAGAAGTAAG CACTTTAAAG GATCTATTTG GACTCGCTAG CAATGATGTC 
TGTTTATTTT TAGAGCATGA CCTCTCAATG GCAAAATACA GCAGGCTGCC TAAGAAAAAG 
GAGAATGAGA AGGTGAAGAC CGAAGTCGGA AAAGAGGTGG CCGCGGCCCG GCGGAAGCAG 
CACCTCTCCT CCCTTCAGTA CTACTGTGCC CTCAACGCGC TGCAGTACAG AAAGCAAATG 
GCCATGATGG AGCCCATGAT AGGCTTTGCC CATGGACAGA TTAACTTTTT TAAGAAGGGA 
GCAGAGATGT TTTCCAAACG TATGGACAGC TTTTTATCCT CCGTTGCAGA CATGGTTCAA 
AGCATTCAGG TAGAACTGGA AGCCGAGGCG GAAAAGATGC GGGTGTCCCA GCAAGAATTA 
CTTTCTGTTG ATGAATCTGT TTACACTCCA GACTCTGATG TGGCCGCACC ACAGATCAAC 
AGGAACCTCA TCCAGAAGGC TGGTTACCTT AATCTTAGAA ACAAAACAGG GCTGGTCACC 
ACCACCTGGG AGAGGCTTTA TTTCTTCACC CAAGGCGGGA ATCTCATGTG TCAGCCCAGG 
GGAGCCGTGG CTGGAGGTTT GATCCAGGAC CTGGACAACT GCTCAGTGAT GGCCGTGGAT 
TGCGAAGACC GGCGCTACTG CTTCCAGATC ACCACGCCCA ATGGAAAATC GGGAATAATC 
CTCCAGGCTG AGAGCAGAAA GGAAAATGAA GAGTGGATAT GTGCAATAAA CAACATCTCC 
AGACAGATCT ACCTGACCGA CAACCCTGAG GCAGTCGCGA TCAAGTTGAA TCAGACCGCT 
CTGCAAGCAG TGACTCCCAT TACAAGTTTT GGAAAAAAAC AAGAAAGCTC ATGCCCCAGC 
CAGAACCTGA AAAATTCAGA GATGGAAAAT GAAAATGACA AGATTGTTCC CAAAGCAACA 
GCCAGTCTAC CTGAAGCAGA GGAGCTGATC GCGCCTGGAA CGCCGATTCA ATTCGATATT 
GTGCTTCCTG CTACAGAATT CCTTGATCAG AACAGAGGGA GCAGGCGTAC CAACCCTTTT 
GGTGAAACTG AGGATGAATC ATTTCCAGAA GCAGAAGATT CTCTTTTGCA GCAGATGTTT 
ATAGTTCGGT TTTTGGGATC AATGGCAGTT AAAACAGACA GCACTACTGA AGTGATTTAT 
GAAGCGATGA GACAAGTATT GGCTGCTCGG GCTATTCATA ACATCTTCCG CATGACAGAA 
TCCCATCTGA TGGTCACCAG TCAATCTTTG AGGTTGATAG ATCCACAGAC TCAAGTATCA 
AGGGCCAATT TTGAACTTAC CAGTGTCACA CAATTTGCTG CTCATCAAGA AAACAAGAGA 
CTGGTTGGTT TTGTCATCCG TGTTCCTGAA TCCACTGGAG AAGAATCTCT GAGTACATAC 
ATTTTTGAAA GCAACTCAGA AGGCGAAAAG ATATGTTATG CTATTAATTT GGGAAAAGAA 
ATTATTGAGG TTCAGAAGGA TCCAGAAGCA CTGGCTCAAT TAATGCTGTC CATACCACTA 
ACCAATGACG GAAAATATGT ACTGTTAAAC GATCAACCAG ATGACGATGA TGGAAATCCA 
AACGAACATA GAGGCGCAGA ATCCGAAGCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( NM_018171.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060641.2 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 1, mRNA.

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CloneID	OHu17777
Clone ID Related Accession (Same CDS sequence)	NM_018171.4 , NM_018171.3 , NM_018171.5
Accession Version	NM_018171.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078874.13 and AC016257.22. On Aug 31, 2019 this sequence version replaced NM_018171.4. Transcript Variant: This variant (1) encodes the predominant isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033731.1, AY113704.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000258530.8/ ENSP00000258530.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCGCCG TGGACAAGCT CCTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GCGTGTTTGA AGAAGATGCT GGCACCCTCA CAGACTATAC CAACCAGCTG 
CTCCAGGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAGA TGTGCCTGGC CACACAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAACTTTG CTCTTGGCAA AGGTGATGAA 
GAAGTAATTT CAACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TCTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CTATCATACA ATTCCGAGAA 
AAGGATCTCA CAGAAGTAAG CACTTTAAAG GATCTATTTG GACTCGCTAG CAATGAGCAT 
GACCTCTCAA TGGCAAAATA CAGCAGGCTG CCTAAGAAAA AGGAGAATGA GAAGGTGAAG 
ACCGAAGTCG GAAAAGAGGT GGCCGCGGCC CGGCGGAAGC AGCACCTCTC CTCCCTTCAG 
TACTACTGTG CCCTCAACGC GCTGCAGTAC AGAAAGCAAA TGGCCATGAT GGAGCCCATG 
ATAGGCTTTG CCCATGGACA GATTAACTTT TTTAAGAAGG GAGCAGAGAT GTTTTCCAAA 
CGTATGGACA GCTTTTTATC CTCCGTTGCA GACATGGTTC AAAGCATTCA GGTAGAACTG 
GAAGCCGAGG CGGAAAAGAT GCGGGTGTCC CAGCAAGAAT TACTTTCTGT TGATGAATCT 
GTTTACACTC CAGACTCTGA TGTGGCCGCA CCACAGATCA ACAGGAACCT CATCCAGAAG 
GCTGGTTACC TTAATCTTAG AAACAAAACA GGGCTGGTCA CCACCACCTG GGAGAGGCTT 
TATTTCTTCA CCCAAGGCGG GAATCTCATG TGTCAGCCCA GGGGAGCCGT GGCTGGAGGT 
TTGATCCAGG ACCTGGACAA CTGCTCAGTG ATGGCCGTGG ATTGCGAAGA CCGGCGCTAC 
TGCTTCCAGA TCACCACGCC CAATGGAAAA TCGGGAATAA TCCTCCAGGC TGAGAGCAGA 
AAGGAAAATG AAGAGTGGAT ATGTGCAATA AACAACATCT CCAGACAGAT CTACCTGACC 
GACAACCCTG AGGCAGTCGC GATCAAGTTG AATCAGACCG CTCTGCAAGC AGTGACTCCC 
ATTACAAGTT TTGGAAAAAA ACAAGAAAGC TCATGCCCCA GCCAGAACCT GAAAAATTCA 
GAGATGGAAA ATGAAAATGA CAAGATTGTT CCCAAAGCAA CAGCCAGTCT ACCTGAAGCA 
GAGGAGCTGA TCGCGCCTGG AACGCCGATT CAATTCGATA TTGTGCTTCC TGCTACAGAA 
TTCCTTGATC AGAACAGAGG GAGCAGGCGT ACCAACCCTT TTGGTGAAAC TGAGGATGAA 
TCATTTCCAG AAGCAGAAGA TTCTCTTTTG CAGCAGATGT TTATAGTTCG GTTTTTGGGA 
TCAATGGCAG TTAAAACAGA CAGCACTACT GAAGTGATTT ATGAAGCGAT GAGACAAGTA 
TTGGCTGCTC GGGCTATTCA TAACATCTTC CGCATGACAG AATCCCATCT GATGGTCACC 
AGTCAATCTT TGAGGTTGAT AGATCCACAG ACTCAAGTAT CAAGGGCCAA TTTTGAACTT 
ACCAGTGTCA CACAATTTGC TGCTCATCAA GAAAACAAGA GACTGGTTGG TTTTGTCATC 
CGTGTTCCTG AATCCACTGG AGAAGAATCT CTGAGTACAT ACATTTTTGA AAGCAACTCA 
GAAGGCGAAA AGATATGTTA TGCTATTAAT TTGGGAAAAG AAATTATTGA GGTTCAGAAG 
GATCCAGAAG CACTGGCTCA ATTAATGCTG TCCATACCAC TAACCAATGA CGGAAAATAT 
GTACTGTTAA ACGATCAACC AGATGACGAT GATGGAAATC CAAACGAACA TAGAGGCGCA 
GAATCCGAAG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060641.2
CDS	163..2157
Translation	MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	NM_018171.5
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018171.5

ORF Insert Sequence:

ATGCCCGCCG TGGACAAGCT CCTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GCGTGTTTGA AGAAGATGCT GGCACCCTCA CAGACTATAC CAACCAGCTG 
CTCCAGGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAGA TGTGCCTGGC CACACAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAACTTTG CTCTTGGCAA AGGTGATGAA 
GAAGTAATTT CAACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TCTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CTATCATACA ATTCCGAGAA 
AAGGATCTCA CAGAAGTAAG CACTTTAAAG GATCTATTTG GACTCGCTAG CAATGAGCAT 
GACCTCTCAA TGGCAAAATA CAGCAGGCTG CCTAAGAAAA AGGAGAATGA GAAGGTGAAG 
ACCGAAGTCG GAAAAGAGGT GGCCGCGGCC CGGCGGAAGC AGCACCTCTC CTCCCTTCAG 
TACTACTGTG CCCTCAACGC GCTGCAGTAC AGAAAGCAAA TGGCCATGAT GGAGCCCATG 
ATAGGCTTTG CCCATGGACA GATTAACTTT TTTAAGAAGG GAGCAGAGAT GTTTTCCAAA 
CGTATGGACA GCTTTTTATC CTCCGTTGCA GACATGGTTC AAAGCATTCA GGTAGAACTG 
GAAGCCGAGG CGGAAAAGAT GCGGGTGTCC CAGCAAGAAT TACTTTCTGT TGATGAATCT 
GTTTACACTC CAGACTCTGA TGTGGCCGCA CCACAGATCA ACAGGAACCT CATCCAGAAG 
GCTGGTTACC TTAATCTTAG AAACAAAACA GGGCTGGTCA CCACCACCTG GGAGAGGCTT 
TATTTCTTCA CCCAAGGCGG GAATCTCATG TGTCAGCCCA GGGGAGCCGT GGCTGGAGGT 
TTGATCCAGG ACCTGGACAA CTGCTCAGTG ATGGCCGTGG ATTGCGAAGA CCGGCGCTAC 
TGCTTCCAGA TCACCACGCC CAATGGAAAA TCGGGAATAA TCCTCCAGGC TGAGAGCAGA 
AAGGAAAATG AAGAGTGGAT ATGTGCAATA AACAACATCT CCAGACAGAT CTACCTGACC 
GACAACCCTG AGGCAGTCGC GATCAAGTTG AATCAGACCG CTCTGCAAGC AGTGACTCCC 
ATTACAAGTT TTGGAAAAAA ACAAGAAAGC TCATGCCCCA GCCAGAACCT GAAAAATTCA 
GAGATGGAAA ATGAAAATGA CAAGATTGTT CCCAAAGCAA CAGCCAGTCT ACCTGAAGCA 
GAGGAGCTGA TCGCGCCTGG AACGCCGATT CAATTCGATA TTGTGCTTCC TGCTACAGAA 
TTCCTTGATC AGAACAGAGG GAGCAGGCGT ACCAACCCTT TTGGTGAAAC TGAGGATGAA 
TCATTTCCAG AAGCAGAAGA TTCTCTTTTG CAGCAGATGT TTATAGTTCG GTTTTTGGGA 
TCAATGGCAG TTAAAACAGA CAGCACTACT GAAGTGATTT ATGAAGCGAT GAGACAAGTA 
TTGGCTGCTC GGGCTATTCA TAACATCTTC CGCATGACAG AATCCCATCT GATGGTCACC 
AGTCAATCTT TGAGGTTGAT AGATCCACAG ACTCAAGTAT CAAGGGCCAA TTTTGAACTT 
ACCAGTGTCA CACAATTTGC TGCTCATCAA GAAAACAAGA GACTGGTTGG TTTTGTCATC 
CGTGTTCCTG AATCCACTGG AGAAGAATCT CTGAGTACAT ACATTTTTGA AAGCAACTCA 
GAAGGCGAAA AGATATGTTA TGCTATTAAT TTGGGAAAAG AAATTATTGA GGTTCAGAAG 
GATCCAGAAG CACTGGCTCA ATTAATGCTG TCCATACCAC TAACCAATGA CGGAAAATAT 
GTACTGTTAA ACGATCAACC AGATGACGAT GATGGAAATC CAAACGAACA TAGAGGCGCA 
GAATCCGAAG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_017019551.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875040.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu83929
Clone ID Related Accession (Same CDS sequence)	XM_017019551.2 , XM_017019551.1
Accession Version	XM_017019551.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017019551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACAC CCCAGACTCG CTCTTTACTG AGCGTGTTTG AAGAAGATGC TGGCACCCTC 
ACAGACTATA CCAACCAGCT GCTCCAGGCA ATGCAGCGCG TCTATGGAGC CCAGAATGAG 
ATGTGCCTGG CCACACAACA GCTTTCTAAG CAACTGCTGG CATATGAAAA ACAGAACTTT 
GCTCTTGGCA AAGGTGATGA AGAAGTAATT TCAACACTCC ACTATTTTTC CAAAGTGGTG 
GATGAGCTTA ATCTTCTCCA TACAGAGCTG GCTAAACAGT TGGCAGACAC AATGGTTCTA 
CCTATCATAC AATTCCGAGA AAAGGATCTC ACAGAAGTAA GCACTTTAAA GGATCTATTT 
GGACTCGCTA GCAATGAGCA TGACCTCTCA ATGGCAAAAT ACAGCAGGCT GCCTAAGAAA 
AAGGAGAATG AGAAGGTGAA GACCGAAGTC GGAAAAGAGG TGGCCGCGGC CCGGCGGAAG 
CAGCACCTCT CCTCCCTTCA GTACTACTGT GCCCTCAACG CGCTGCAGTA CAGAAAGCAA 
ATGGCCATGA TGGAGCCCAT GATAGGCTTT GCCCATGGAC AGATTAACTT TTTTAAGAAG 
GGAGCAGAGA TGTTTTCCAA ACGTATGGAC AGCTTTTTAT CCTCCGTTGC AGACATGGTT 
CAAAGCATTC AGGTAGAACT GGAAGCCGAG GCGGAAAAGA TGCGGGTGTC CCAGCAAGAA 
TTACTTTCTG TTGATGAATC TGTTTACACT CCAGACTCTG ATGTGGCCGC ACCACAGATC 
AACAGGAACC TCATCCAGAA GGCTGGTTAC CTTAATCTTA GAAACAAAAC AGGGCTGGTC 
ACCACCACCT GGGAGAGGCT TTATTTCTTC ACCCAAGGCG GGAATCTCAT GTGTCAGCCC 
AGGGGAGCCG TGGCTGGAGG TTTGATCCAG GACCTGGACA ACTGCTCAGT GATGGCCGTG 
GATTGCGAAG ACCGGCGCTA CTGCTTCCAG ATCACCACGC CCAATGGAAA ATCGGGAATA 
ATCCTCCAGG CTGAGAGCAG AAAGGAAAAT GAAGAGTGGA TATGTGCAAT AAACAACATC 
TCCAGACAGA TCTACCTGAC CGACAACCCT GAGGCAGTCG CGATCAAGTT GAATCAGACC 
GCTCTGCAAG CAGTGACTCC CATTACAAGT TTTGGAAAAA AACAAGAAAG CTCATGCCCC 
AGCCAGAACC TGAAAAATTC AGAGATGGAA AATGAAAATG ACAAGATTGT TCCCAAAGCA 
ACAGCCAGTC TACCTGAAGC AGAGGAGCTG ATCGCGCCTG GAACGCCGAT TCAATTCGAT 
ATTGTGCTTC CTGCTACAGA ATTCCTTGAT CAGAACAGAG GGAGCAGGCG TACCAACCCT 
TTTGGTGAAA CTGAGGATGA ATCATTTCCA GAAGCAGAAG ATTCTCTTTT GCAGCAGATG 
TTTATAGTTC GGTTTTTGGG ATCAATGGCA GTTAAAACAG ACAGCACTAC TGAAGTGATT 
TATGAAGCGA TGAGACAAGT ATTGGCTGCT CGGGCTATTC ATAACATCTT CCGCATGACA 
GAATCCCATC TGATGGTCAC CAGTCAATCT TTGAGGTTGA TAGATCCACA GACTCAAGTA 
TCAAGGGCCA ATTTTGAACT TACCAGTGTC ACACAATTTG CTGCTCATCA AGAAAACAAG 
AGACTGGTTG GTTTTGTCAT CCGTGTTCCT GAATCCACTG GAGAAGAATC TCTGAGTACA 
TACATTTTTG AAAGCAACTC AGAAGGCGAA AAGATATGTT ATGCTATTAA TTTGGGAAAA 
GAAATTATTG AGGTTCAGAA GGATCCAGAA GCACTGGCTC AATTAATGCT GTCCATACCA 
CTAACCAATG ACGGAAAATA TGTACTGTTA AACGATCAAC CAGATGACGA TGATGGAAAT 
CCAAACGAAC ATAGAGGCGC AGAATCCGAA GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875040.1
CDS	663..2618
Translation	MTTPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	XM_017019551.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019551.2

ORF Insert Sequence:

ATGACCACAC CCCAGACTCG CTCTTTACTG AGCGTGTTTG AAGAAGATGC TGGCACCCTC 
ACAGACTATA CCAACCAGCT GCTCCAGGCA ATGCAGCGCG TCTATGGAGC CCAGAATGAG 
ATGTGCCTGG CCACACAACA GCTTTCTAAG CAACTGCTGG CATATGAAAA ACAGAACTTT 
GCTCTTGGCA AAGGTGATGA AGAAGTAATT TCAACACTCC ACTATTTTTC CAAAGTGGTG 
GATGAGCTTA ATCTTCTCCA TACAGAGCTG GCTAAACAGT TGGCAGACAC AATGGTTCTA 
CCTATCATAC AATTCCGAGA AAAGGATCTC ACAGAAGTAA GCACTTTAAA GGATCTATTT 
GGACTCGCTA GCAATGAGCA TGACCTCTCA ATGGCAAAAT ACAGCAGGCT GCCTAAGAAA 
AAGGAGAATG AGAAGGTGAA GACCGAAGTC GGAAAAGAGG TGGCCGCGGC CCGGCGGAAG 
CAGCACCTCT CCTCCCTTCA GTACTACTGT GCCCTCAACG CGCTGCAGTA CAGAAAGCAA 
ATGGCCATGA TGGAGCCCAT GATAGGCTTT GCCCATGGAC AGATTAACTT TTTTAAGAAG 
GGAGCAGAGA TGTTTTCCAA ACGTATGGAC AGCTTTTTAT CCTCCGTTGC AGACATGGTT 
CAAAGCATTC AGGTAGAACT GGAAGCCGAG GCGGAAAAGA TGCGGGTGTC CCAGCAAGAA 
TTACTTTCTG TTGATGAATC TGTTTACACT CCAGACTCTG ATGTGGCCGC ACCACAGATC 
AACAGGAACC TCATCCAGAA GGCTGGTTAC CTTAATCTTA GAAACAAAAC AGGGCTGGTC 
ACCACCACCT GGGAGAGGCT TTATTTCTTC ACCCAAGGCG GGAATCTCAT GTGTCAGCCC 
AGGGGAGCCG TGGCTGGAGG TTTGATCCAG GACCTGGACA ACTGCTCAGT GATGGCCGTG 
GATTGCGAAG ACCGGCGCTA CTGCTTCCAG ATCACCACGC CCAATGGAAA ATCGGGAATA 
ATCCTCCAGG CTGAGAGCAG AAAGGAAAAT GAAGAGTGGA TATGTGCAAT AAACAACATC 
TCCAGACAGA TCTACCTGAC CGACAACCCT GAGGCAGTCG CGATCAAGTT GAATCAGACC 
GCTCTGCAAG CAGTGACTCC CATTACAAGT TTTGGAAAAA AACAAGAAAG CTCATGCCCC 
AGCCAGAACC TGAAAAATTC AGAGATGGAA AATGAAAATG ACAAGATTGT TCCCAAAGCA 
ACAGCCAGTC TACCTGAAGC AGAGGAGCTG ATCGCGCCTG GAACGCCGAT TCAATTCGAT 
ATTGTGCTTC CTGCTACAGA ATTCCTTGAT CAGAACAGAG GGAGCAGGCG TACCAACCCT 
TTTGGTGAAA CTGAGGATGA ATCATTTCCA GAAGCAGAAG ATTCTCTTTT GCAGCAGATG 
TTTATAGTTC GGTTTTTGGG ATCAATGGCA GTTAAAACAG ACAGCACTAC TGAAGTGATT 
TATGAAGCGA TGAGACAAGT ATTGGCTGCT CGGGCTATTC ATAACATCTT CCGCATGACA 
GAATCCCATC TGATGGTCAC CAGTCAATCT TTGAGGTTGA TAGATCCACA GACTCAAGTA 
TCAAGGGCCA ATTTTGAACT TACCAGTGTC ACACAATTTG CTGCTCATCA AGAAAACAAG 
AGACTGGTTG GTTTTGTCAT CCGTGTTCCT GAATCCACTG GAGAAGAATC TCTGAGTACA 
TACATTTTTG AAAGCAACTC AGAAGGCGAA AAGATATGTT ATGCTATTAA TTTGGGAAAA 
GAAATTATTG AGGTTCAGAA GGATCCAGAA GCACTGGCTC AATTAATGCT GTCCATACCA 
CTAACCAATG ACGGAAAATA TGTACTGTTA AACGATCAAC CAGATGACGA TGATGGAAAT 
CCAAACGAAC ATAGAGGCGC AGAATCCGAA GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_017019551.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875040.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu83929
Clone ID Related Accession (Same CDS sequence)	XM_017019551.2 , XM_017019551.1
Accession Version	XM_017019551.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACAC CCCAGACTCG CTCTTTACTG AGCGTGTTTG AAGAAGATGC TGGCACCCTC 
ACAGACTATA CCAACCAGCT GCTCCAGGCA ATGCAGCGCG TCTATGGAGC CCAGAATGAG 
ATGTGCCTGG CCACACAACA GCTTTCTAAG CAACTGCTGG CATATGAAAA ACAGAACTTT 
GCTCTTGGCA AAGGTGATGA AGAAGTAATT TCAACACTCC ACTATTTTTC CAAAGTGGTG 
GATGAGCTTA ATCTTCTCCA TACAGAGCTG GCTAAACAGT TGGCAGACAC AATGGTTCTA 
CCTATCATAC AATTCCGAGA AAAGGATCTC ACAGAAGTAA GCACTTTAAA GGATCTATTT 
GGACTCGCTA GCAATGAGCA TGACCTCTCA ATGGCAAAAT ACAGCAGGCT GCCTAAGAAA 
AAGGAGAATG AGAAGGTGAA GACCGAAGTC GGAAAAGAGG TGGCCGCGGC CCGGCGGAAG 
CAGCACCTCT CCTCCCTTCA GTACTACTGT GCCCTCAACG CGCTGCAGTA CAGAAAGCAA 
ATGGCCATGA TGGAGCCCAT GATAGGCTTT GCCCATGGAC AGATTAACTT TTTTAAGAAG 
GGAGCAGAGA TGTTTTCCAA ACGTATGGAC AGCTTTTTAT CCTCCGTTGC AGACATGGTT 
CAAAGCATTC AGGTAGAACT GGAAGCCGAG GCGGAAAAGA TGCGGGTGTC CCAGCAAGAA 
TTACTTTCTG TTGATGAATC TGTTTACACT CCAGACTCTG ATGTGGCCGC ACCACAGATC 
AACAGGAACC TCATCCAGAA GGCTGGTTAC CTTAATCTTA GAAACAAAAC AGGGCTGGTC 
ACCACCACCT GGGAGAGGCT TTATTTCTTC ACCCAAGGCG GGAATCTCAT GTGTCAGCCC 
AGGGGAGCCG TGGCTGGAGG TTTGATCCAG GACCTGGACA ACTGCTCAGT GATGGCCGTG 
GATTGCGAAG ACCGGCGCTA CTGCTTCCAG ATCACCACGC CCAATGGAAA ATCGGGAATA 
ATCCTCCAGG CTGAGAGCAG AAAGGAAAAT GAAGAGTGGA TATGTGCAAT AAACAACATC 
TCCAGACAGA TCTACCTGAC CGACAACCCT GAGGCAGTCG CGATCAAGTT GAATCAGACC 
GCTCTGCAAG CAGTGACTCC CATTACAAGT TTTGGAAAAA AACAAGAAAG CTCATGCCCC 
AGCCAGAACC TGAAAAATTC AGAGATGGAA AATGAAAATG ACAAGATTGT TCCCAAAGCA 
ACAGCCAGTC TACCTGAAGC AGAGGAGCTG ATCGCGCCTG GAACGCCGAT TCAATTCGAT 
ATTGTGCTTC CTGCTACAGA ATTCCTTGAT CAGAACAGAG GGAGCAGGCG TACCAACCCT 
TTTGGTGAAA CTGAGGATGA ATCATTTCCA GAAGCAGAAG ATTCTCTTTT GCAGCAGATG 
TTTATAGTTC GGTTTTTGGG ATCAATGGCA GTTAAAACAG ACAGCACTAC TGAAGTGATT 
TATGAAGCGA TGAGACAAGT ATTGGCTGCT CGGGCTATTC ATAACATCTT CCGCATGACA 
GAATCCCATC TGATGGTCAC CAGTCAATCT TTGAGGTTGA TAGATCCACA GACTCAAGTA 
TCAAGGGCCA ATTTTGAACT TACCAGTGTC ACACAATTTG CTGCTCATCA AGAAAACAAG 
AGACTGGTTG GTTTTGTCAT CCGTGTTCCT GAATCCACTG GAGAAGAATC TCTGAGTACA 
TACATTTTTG AAAGCAACTC AGAAGGCGAA AAGATATGTT ATGCTATTAA TTTGGGAAAA 
GAAATTATTG AGGTTCAGAA GGATCCAGAA GCACTGGCTC AATTAATGCT GTCCATACCA 
CTAACCAATG ACGGAAAATA TGTACTGTTA AACGATCAAC CAGATGACGA TGATGGAAAT 
CCAAACGAAC ATAGAGGCGC AGAATCCGAA GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875040.1
CDS	663..2618
Translation	MTTPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	XM_017019551.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019551.1

ORF Insert Sequence:

ATGACCACAC CCCAGACTCG CTCTTTACTG AGCGTGTTTG AAGAAGATGC TGGCACCCTC 
ACAGACTATA CCAACCAGCT GCTCCAGGCA ATGCAGCGCG TCTATGGAGC CCAGAATGAG 
ATGTGCCTGG CCACACAACA GCTTTCTAAG CAACTGCTGG CATATGAAAA ACAGAACTTT 
GCTCTTGGCA AAGGTGATGA AGAAGTAATT TCAACACTCC ACTATTTTTC CAAAGTGGTG 
GATGAGCTTA ATCTTCTCCA TACAGAGCTG GCTAAACAGT TGGCAGACAC AATGGTTCTA 
CCTATCATAC AATTCCGAGA AAAGGATCTC ACAGAAGTAA GCACTTTAAA GGATCTATTT 
GGACTCGCTA GCAATGAGCA TGACCTCTCA ATGGCAAAAT ACAGCAGGCT GCCTAAGAAA 
AAGGAGAATG AGAAGGTGAA GACCGAAGTC GGAAAAGAGG TGGCCGCGGC CCGGCGGAAG 
CAGCACCTCT CCTCCCTTCA GTACTACTGT GCCCTCAACG CGCTGCAGTA CAGAAAGCAA 
ATGGCCATGA TGGAGCCCAT GATAGGCTTT GCCCATGGAC AGATTAACTT TTTTAAGAAG 
GGAGCAGAGA TGTTTTCCAA ACGTATGGAC AGCTTTTTAT CCTCCGTTGC AGACATGGTT 
CAAAGCATTC AGGTAGAACT GGAAGCCGAG GCGGAAAAGA TGCGGGTGTC CCAGCAAGAA 
TTACTTTCTG TTGATGAATC TGTTTACACT CCAGACTCTG ATGTGGCCGC ACCACAGATC 
AACAGGAACC TCATCCAGAA GGCTGGTTAC CTTAATCTTA GAAACAAAAC AGGGCTGGTC 
ACCACCACCT GGGAGAGGCT TTATTTCTTC ACCCAAGGCG GGAATCTCAT GTGTCAGCCC 
AGGGGAGCCG TGGCTGGAGG TTTGATCCAG GACCTGGACA ACTGCTCAGT GATGGCCGTG 
GATTGCGAAG ACCGGCGCTA CTGCTTCCAG ATCACCACGC CCAATGGAAA ATCGGGAATA 
ATCCTCCAGG CTGAGAGCAG AAAGGAAAAT GAAGAGTGGA TATGTGCAAT AAACAACATC 
TCCAGACAGA TCTACCTGAC CGACAACCCT GAGGCAGTCG CGATCAAGTT GAATCAGACC 
GCTCTGCAAG CAGTGACTCC CATTACAAGT TTTGGAAAAA AACAAGAAAG CTCATGCCCC 
AGCCAGAACC TGAAAAATTC AGAGATGGAA AATGAAAATG ACAAGATTGT TCCCAAAGCA 
ACAGCCAGTC TACCTGAAGC AGAGGAGCTG ATCGCGCCTG GAACGCCGAT TCAATTCGAT 
ATTGTGCTTC CTGCTACAGA ATTCCTTGAT CAGAACAGAG GGAGCAGGCG TACCAACCCT 
TTTGGTGAAA CTGAGGATGA ATCATTTCCA GAAGCAGAAG ATTCTCTTTT GCAGCAGATG 
TTTATAGTTC GGTTTTTGGG ATCAATGGCA GTTAAAACAG ACAGCACTAC TGAAGTGATT 
TATGAAGCGA TGAGACAAGT ATTGGCTGCT CGGGCTATTC ATAACATCTT CCGCATGACA 
GAATCCCATC TGATGGTCAC CAGTCAATCT TTGAGGTTGA TAGATCCACA GACTCAAGTA 
TCAAGGGCCA ATTTTGAACT TACCAGTGTC ACACAATTTG CTGCTCATCA AGAAAACAAG 
AGACTGGTTG GTTTTGTCAT CCGTGTTCCT GAATCCACTG GAGAAGAATC TCTGAGTACA 
TACATTTTTG AAAGCAACTC AGAAGGCGAA AAGATATGTT ATGCTATTAA TTTGGGAAAA 
GAAATTATTG AGGTTCAGAA GGATCCAGAA GCACTGGCTC AATTAATGCT GTCCATACCA 
CTAACCAATG ACGGAAAATA TGTACTGTTA AACGATCAAC CAGATGACGA TGATGGAAAT 
CCAAACGAAC ATAGAGGCGC AGAATCCGAA GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_017019554.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875043.1 Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X8, mRNA.

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CloneID	OHu83930
Clone ID Related Accession (Same CDS sequence)	XM_017019554.1
Accession Version	XM_017019554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DCC-interacting protein 13-beta isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCCG TGGACAAGCT CCTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GCGTGTTTGA AGAAGATGCT GGCACCCTCA CAGACTATAC CAACCAGCTG 
CTCCAGGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAGA TGTGCCTGGC CACACAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAACTTTG CTCTTGGCAA AGGTGATGAA 
GAAGTAATTT CAACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TCTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CTATCATACA ATTCCGAGAA 
AAGGATCTCA CAGAAGTAAG CACTTTAAAG GATCTATTTG GACTCGCTAG CAATGAGCAT 
GACCTCTCAA TGGCAAAATA CAGCAGGCTG CCTAAGAAAA AGGAGAATGA GAAGGTGAAG 
ACCGAAGTCG GAAAAGAGGT GGCCGCGGCC CGGCGGAAGC AGCACCTCTC CTCCCTTCAG 
TACTACTGTG CCCTCAACGC GCTGCAGTAC AGAAAGCAAA TGGCCATGAT GGAGCCCATG 
ATAGGCTTTG CCCATGGACA GATTAACTTT TTTAAGAAGG GAGCAGAGAT GTTTTCCAAA 
CGTATGGACA GCTTTTTATC CTCCGTTGCA GACATGGTTC AAAGCATTCA GGTAGAACTG 
GAAGCCGAGG CGGAAAAGAT GCGGGTGTCC CAGCAAGAAT TACTTTCTGT TGATGAATCT 
GTTTACACTC CAGACTCTGA TGTGGCCGCA CCACAGATCA ACAGGAACCT CATCCAGAAG 
GCTGGTTACC TTAATCTTAG AAACAAAACA GGGCTGGTCA CCACCACCTG GGAGAGGCTT 
TATTTCTTCA CCCAAGGCGG GAATCTCATG TGTCAGCCCA GGGGAGCCGT GGCTGGAGGT 
TTGATCCAGG ACCTGGACAA CTGCTCAGTG ATGGCCGTGG ATTGCGAAGA CCGGCGCTAC 
TGCTTCCAGA TCACCACGCC CAATGGAAAA TCGGGAATAA TCCTCCAGGC TGAGAGCAGA 
AAGGAAAATG AAGAGTGGAT ATGTGCAATA AACAACATCT CCAGACAGAT CTACCTGACC 
GACAACCCTG AGGCAGTCGC GATCAAGTTG AATCAGACCG CTCTGCAAGC AGTGACTCCC 
ATTACAAGTT TTGGAAAAAA ACAAGAAAGC TCATGCCCCA GCCAGAACCT GAAAAATTCA 
GAGATGGAAA ATGAAAATGA CAAGATTGTT CCCAAAGCAA CAGCCAGTCT ACCTGAAGCA 
GAGGAGCTGA TCGCGCCTGG AACGCCGATT CAATTCGATA TTGTGCTTCC TGCTACAGAA 
TTCCTTGATC AGAACAGAGG GAGCAGGCGT ACCAACCCTT TTGGTGAAAC TGAGGATGAA 
TCATTTCCAG AAGCAGAAGA TTCTCTTTTG CAGCAGATGT TTATAGTTCG GTTTTTGGGA 
TCAATGGCAG TTAAAACAGA CAGCACTACT GAAGTGATTT ATGAAGCGAT GAGACAAGTA 
TTGGCTGCTC GGGCTATTCA TAACATCTTC CGCATGACAG AATCCCATCT GATGGTCACC 
AGTCAATCTT TGAGGTTGAT AGATCCACAG ACTCAAGTAT CAAGGGCCAA TATATGTTAT 
GCTATTAATT TGGGAAAAGA AATTATTGAG GTTCAGAAGG ATCCAGAAGC ACTGGCTCAA 
TTAATGCTGT CCATACCACT AACCAATGAC GGAAAATATG TACTGTTAAA CGATCAACCA 
GATGACGATG ATGGAAATCC AAACGAACAT AGAGGCGCAG AATCCGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875043.1
CDS	138..1991
Translation	MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

Target ORF information:

RefSeq Version	XM_017019554.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019554.1

ORF Insert Sequence:

ATGCCCGCCG TGGACAAGCT CCTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GCGTGTTTGA AGAAGATGCT GGCACCCTCA CAGACTATAC CAACCAGCTG 
CTCCAGGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAGA TGTGCCTGGC CACACAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAACTTTG CTCTTGGCAA AGGTGATGAA 
GAAGTAATTT CAACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TCTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CTATCATACA ATTCCGAGAA 
AAGGATCTCA CAGAAGTAAG CACTTTAAAG GATCTATTTG GACTCGCTAG CAATGAGCAT 
GACCTCTCAA TGGCAAAATA CAGCAGGCTG CCTAAGAAAA AGGAGAATGA GAAGGTGAAG 
ACCGAAGTCG GAAAAGAGGT GGCCGCGGCC CGGCGGAAGC AGCACCTCTC CTCCCTTCAG 
TACTACTGTG CCCTCAACGC GCTGCAGTAC AGAAAGCAAA TGGCCATGAT GGAGCCCATG 
ATAGGCTTTG CCCATGGACA GATTAACTTT TTTAAGAAGG GAGCAGAGAT GTTTTCCAAA 
CGTATGGACA GCTTTTTATC CTCCGTTGCA GACATGGTTC AAAGCATTCA GGTAGAACTG 
GAAGCCGAGG CGGAAAAGAT GCGGGTGTCC CAGCAAGAAT TACTTTCTGT TGATGAATCT 
GTTTACACTC CAGACTCTGA TGTGGCCGCA CCACAGATCA ACAGGAACCT CATCCAGAAG 
GCTGGTTACC TTAATCTTAG AAACAAAACA GGGCTGGTCA CCACCACCTG GGAGAGGCTT 
TATTTCTTCA CCCAAGGCGG GAATCTCATG TGTCAGCCCA GGGGAGCCGT GGCTGGAGGT 
TTGATCCAGG ACCTGGACAA CTGCTCAGTG ATGGCCGTGG ATTGCGAAGA CCGGCGCTAC 
TGCTTCCAGA TCACCACGCC CAATGGAAAA TCGGGAATAA TCCTCCAGGC TGAGAGCAGA 
AAGGAAAATG AAGAGTGGAT ATGTGCAATA AACAACATCT CCAGACAGAT CTACCTGACC 
GACAACCCTG AGGCAGTCGC GATCAAGTTG AATCAGACCG CTCTGCAAGC AGTGACTCCC 
ATTACAAGTT TTGGAAAAAA ACAAGAAAGC TCATGCCCCA GCCAGAACCT GAAAAATTCA 
GAGATGGAAA ATGAAAATGA CAAGATTGTT CCCAAAGCAA CAGCCAGTCT ACCTGAAGCA 
GAGGAGCTGA TCGCGCCTGG AACGCCGATT CAATTCGATA TTGTGCTTCC TGCTACAGAA 
TTCCTTGATC AGAACAGAGG GAGCAGGCGT ACCAACCCTT TTGGTGAAAC TGAGGATGAA 
TCATTTCCAG AAGCAGAAGA TTCTCTTTTG CAGCAGATGT TTATAGTTCG GTTTTTGGGA 
TCAATGGCAG TTAAAACAGA CAGCACTACT GAAGTGATTT ATGAAGCGAT GAGACAAGTA 
TTGGCTGCTC GGGCTATTCA TAACATCTTC CGCATGACAG AATCCCATCT GATGGTCACC 
AGTCAATCTT TGAGGTTGAT AGATCCACAG ACTCAAGTAT CAAGGGCCAA TATATGTTAT 
GCTATTAATT TGGGAAAAGA AATTATTGAG GTTCAGAAGG ATCCAGAAGC ACTGGCTCAA 
TTAATGCTGT CCATACCACT AACCAATGAC GGAAAATATG TACTGTTAAA CGATCAACCA 
GATGACGATG ATGGAAATCC AAACGAACAT AGAGGCGCAG AATCCGAAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Alternative splicing of the OCC-1 gene generates three splice variants and a novel exonic microRNA, which regulate the Wnt signaling pathway.
RNA (New York, N.Y.)23(1)70-85(2017 01)
Najafi H,Soltani BM,Dokanehiifard S,Nasiri S,Mowla SJ

An inter-species protein-protein interaction network across vast evolutionary distance.
Molecular systems biology12(4)865(2016 Apr)
Zhong Q,Pevzner SJ,Hao T,Wang Y,Mosca R,Menche J,Taipale M,Ta?an M,Fan C,Yang X,Haley P,Murray RR,Mer F,Gebreab F,Tam S,MacWilliams A,Dricot A,Reichert P,Santhanam B,Ghamsari L,Calderwood MA,Rolland T,Charloteaux B,Lindquist S,Barab?si AL,Hill DE,Aloy P,Cusick ME,Xia Y,Roth FP,Vidal M

APPL proteins promote TGF?-induced nuclear transport of the TGF? type I receptor intracellular domain.
Oncotarget7(1)279-92(2016 Jan)
Song J,Mu Y,Li C,Bergh A,Miaczynska M,Heldin CH,Landstr?m M

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APPL2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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見積依頼

Eメール

電話

FAX

オンラインフォーム